] EDTA decal
Hi Colleen,
I would say it's unusual, but not completely impossible that EDTA has
interfered with your IHC. We had that problem with demonstrating
B-galactosidase in mouse bones. If we decalcified it in EDTA after whole
mount staining in X-gal, the blue staining was removed. But if we
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Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] EDTA decal
Hi Colleen,
I would say it's unusual, but not completely impossible that EDTA has
interfered with your IHC. We had that problem with demonstrating
B-galactosidase in mouse bones. If we decalcified it in EDTA after whole
Hi Colleen,
I would say it's unusual, but not completely impossible that EDTA has
interfered with your IHC. We had that problem with demonstrating
B-galactosidase in mouse bones. If we decalcified it in EDTA after whole mount
staining in X-gal, the blue staining was removed. But if we
Hi Patti,
Years ago, I used a EDTA Decal Solution which was recommended by Dr. Jaffee, a
Hematopathologist at NIH. This formula was particularly great for BM Core
BX's. It is much gentler on the tissue, and is less likely to harm the
cellular integrity. Dr. Todd Barry also prefers the use of
