[Histonet] GMS Failures on Ventana BMSS

Happy Solstice, Happy Friday, We are having a lot of GMS staining run failures on our BenchMark Special Stainers. The problem is not predictable. It's not slide position related because one day position 2 (example) works, the next run it doesn't. This is only happening with the GMS stains. Ha

Re: [Histonet] GMS Positive Fungal Tissue Needed

Hi Kelly We have blocks that you could have. We are currently looking for gram negative, and AFB positive controls if you have any please let us know. Please let us know if you have any of those and we can work something out. Thanks, Leonard Ringo Macneal Hospital Anatomic Pathology 323

[Histonet] GMS Positive Fungal Tissue Needed

Good Afternoon, We are in need of some GMS positive fungus tissue for positive controls. Would anyone out there happen to have some blocks we could have? We would be willing to swap if we had something you needed. We would also cover the cost of shipping. Thanks, Kelly Kelly Pairan, HT (AS

Re: [Histonet] GMS

Maybe a fixation issue? Is this happening on all your slides, or just one set. Also, do you have a little acetic acid in the light green, I find that helps. I also find it washes out quickly and a fast dehydration is necessary. Very funny that you are having issues with the LG and not the silver,

[Histonet] GMS

I am having difficulties with a stain I've done many times ...GMS. The light green is only staining the outer edges of the specimen. Any Ideas? Thanks Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/ma

Re: [Histonet] GMS silver stain on frozen tissue

ntrol. We also run a tissue Gram stain on these samples and report the results in about two hours. From: Pedro Louro via Histonet [histonet@lists.utsouthwestern.edu] Sent: Wednesday, November 11, 2015 11:31 AM To: histonet@lists.utsouthwestern.edu Subject:

[Histonet] GMS silver stain on frozen tissue

Hi everyone, Has anyone experimented with performing a Grocott Methenamine Silver stain on frozen tissues? I have a PI that is requesting this but I cannot find any literature on frozen tissue. Any help or ideas is appreciated. Thanks in advance, Pedro _

[Histonet] GMS and Gram stains

Hi All Netters, I need to get a better GMS and Gram stain. If anyone could share their protocols where they get the solutions that would be fantastic. Thanks, Mehndi Helgren Dominion Pathology Labs Norfolk, VA (AKA Hurricane Central) :) ___ Histo

[Histonet] GMS Control

We are in need of a GMS control for fungus. We have an excellent AFB control to trade. Please contact me if you are able to help. Thanks. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com www.c

Re: [Histonet] GMS question

Gayle Callis's discourse on GMS staining is must reading for anyone who does the GMS stain. Freida Carson in 1999 showed the importance of using chromic acid - I was distressed that she didn't cite this paper in the 4th edition of her book. The most rigorous test of the GMS stain is probably the d

Re: [Histonet] GMS Question

om: Goins, Tresa [tgo...@mt.gov] Sent: Saturday, 2 May 2015 3:28 AM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GMS Question To get a positive PAS or GMS fungal stain, one must oxidize the carbohydrate in the fungal cell wall. Chromic acid is a stronger oxidizer than

Re: [Histonet] GMS Question

AUSTRALIA From: Paula Lucas [plu...@biopath.org] Sent: Saturday, 2 May 2015 12:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS Question Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I

Re: [Histonet] GMS Question

n.net] Sent: Friday, May 01, 2015 1:17 PM To: Goins, Tresa; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GMS Question Hi Tresa, What staining parameters do you suggest for seeing mature and/or immature fungal cell walls? I don't think you will know what level of maturity is pres

Re: [Histonet] GMS Question

ain either mature or immature fungal and/or both cell wall structure. Gayle Callis HTL/HT/MT (ASCP) -Original Message- From: Goins, Tresa [mailto:tgo...@mt.gov] Sent: Friday, May 01, 2015 11:29 AM To: Paula Lucas; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] GMS Question

Re: [Histonet] GMS Question

get a false negative because the carbohydrate structure no longer resembles a fungal cell wall. Tresa -Original Message- From: Paula Lucas [mailto:plu...@biopath.org] Sent: Friday, May 01, 2015 8:17 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS Question Hello, I

[Histonet] GMS Question

Hello, I think I already know the answer but I'm not sure why so if someone can help me understand the theory behind it, I would greatly appreciate it. Currently, we use the Richard Allen kit for the GMS stain and it uses Periodic Acid as the 1st step. We use a control tissue from a case

[Histonet] GMS control

Thank you to all who volunteered ideas and control blocks. Of course, the control must be of human origin, so they are digging through their files to find a nice fungusy patient tissue for me. Darn those CAP regulations, they take all the fun out of it. I still want to try an orange or Slim Jim

RE: [EXTERNAL] [Histonet] GMS controls?

58 AM To: Paula Sicurello; HistoNet Subject: RE: [EXTERNAL] [Histonet] GMS controls? I have experimented on some things and found Blue Cheese to be excellent source of not only fungus but yeast and some bacteria. I did make a couple of smears with the blue cheese but seemed to do much better

RE: [EXTERNAL] [Histonet] GMS controls?

. Sincerely, Sherrian McAnn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, March 17, 2015 5:51 PM To: HistoNet Subject: [EXTERNAL] [Histonet] GMS controls? Good Afternoon Netters

[Histonet] GMS controls?

Good Afternoon Netters, I have a project to validate a special stainer and will be starting with GMS. What can I use as a good source of fungus? Was it last week there was mention of orange peels and onions? I need to get the OK of the attending pathologist, so I probably can't use anything too

[Histonet] GMS better than PAS for Fungi

Thought that the subject title should be changed. (References available on request) No, Using Periodic acid instead of chromic acid just gives you a PASM. Pseudo-fungi are PAS Positive but classic GMS (using chromic acid) negative. The literature is quite confusing on pseudo-fungi. Some say that

[Histonet] GMS fungus controls

Jim, We get our Fungus, GMS Aspergillus controls (slides) and many of our hard to find control slides from Newcomer Supply 1-800-383-7799 or 608-831-7888 Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholi

[Histonet] GMS fungus control

Second question of the day: what is everyone using for their fungus control for GMS? We have always used pneumocystis when looking for pneumo, and Aspergillus when looking for fungus, but wondering if anyone uses any type of fungus just to see that stain worked properly. We are having difficu

[Histonet] GMS stain on blood smear

I have an aspirate from a mass on an animal. It was very bloody, I have spun down a portion of the sample. Does it need to be fixed with alcohol before I make slide smears? I need to do a GMS on a direct smear. What are the staining steps I need to do for these smears? Any info would be greatl

RE: [Histonet] GMS on toenails

f Of Santiago, Albert Sent: Monday, April 23, 2012 8:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on toenails From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 P

[Histonet] GMS on toenails

From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail

RE: [Histonet] GMS on Toenail

, April 19, 2012 3:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GMS on Toenail Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the

[Histonet] GMS on Toenail

Hello to all in histoland. We have a stubborn toenail that keeps coming off when we try to do a GMS stain on the ventana machine. Any suggestions on how to keep the section on the slide during the staining procedure. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONF

Re: [Histonet] GMS stain

Hi Margaret, What problem are you experiencing ? Are you over impregnated or under impregnated ? At what thickness do you cut your Section ? Ideally 3 to 4 microns How do you make up you Hexamine solution ? if you get contamination of Hexamine solution make sure that glass ware is clean. Try this

[Histonet] GMS stain

We sometimes have problems with the stain if we use positive slides. Margaret ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] GMS on decal tissue

Has anyone seen or heard of problems with Grocott Methenamine Silver staining for fungi on decal tissues? Specifically, background or false positives? I can't find anything in any books that gives any indications to not use decaled tissue. Thanks! Tim Morken Supervisor, Histology / IPOX UCSF M

[Histonet] GMS Fungus control

I wondering if anybody out there would be willing to trade GMS fungus controls for AFB controls. Please contact me if interested. Jason McGough HT(ASCP) Account Representative - Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 Ex

RE: [Histonet] GMS-Fungus on nails

/24/2009 10:30 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formali

Re: [Histonet] GMS-Fungus on nails

lot more gentler on the tissue that the GMS. We are the toenail lab for the U.S. Air Force , Joe "The Toe" - Original Message - From: "jstaruk" To: "'Clare Thornton'" ; Sent: Friday, July 24, 2009 10:41 AM Subject: RE: [Histonet] GMS-Fung

[Histonet] GMS-Fungus on nails

Under separate cover I am sinding you my procedure. René J. --- On Fri, 7/24/09, Clare Thornton wrote: From: Clare Thornton Subject: [Histonet] GMS-Fungus on nails To: "'histonet@lists.utsouthwestern.edu'" Date: Friday, July 24, 2009, 11:30 AM We do a GMS for fungus on

RE: [Histonet] GMS-Fungus on nails

Friday, July 24, 2009 11:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formali

RE: [Histonet] GMS-Fungus on nails

...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 9:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the sli

RE: [Histonet] GMS-Fungus on nails

24, 2009 9:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, usin

RE: [Histonet] GMS-Fungus on nails

www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Friday, July 24, 2009 11:31 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] GMS-Fungus on nails We do

[Histonet] GMS-Fungus on nails

We do a GMS for fungus on all nails we receive. We often have a difficult time keeping the nail tissue on the slide. We've tried baking for long periods, pre-treating in formalin, using silane slides, with no luck. Even when the nails cut relatively easily we still lose it, and end up running

[Histonet] GMS Control

Anyone have any non-Aspergillus control block you would share with us? We surely would appreciate it! Thanks, j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax Confidentiality Notice: This

[Histonet] GMS Contamination

Dear Histonet, We are currently using Ventana Nexes Special Stain modules for our GMS stains. Our pathologists have been seeing bacteria all over the stains. We decontaminate our machines monthly (which is more than Ventana recommends) and make our solutions up fresh everyday. Yet the contamina