Hello Histonet!
Thank you all for your time and wonderful suggestions. This is the very
first time, I have to work with paraffin embedded brain tissues.
Based on the suggestions from Histonet colleagues, I have decided to
obtain 200 um thick brain sections for processing and embedding. Since I
ha
I believe amyloid does fade with age on cut slides. Frieda sited Bancroft and
Cook that intensity decreases with age and "long-standing" deposits stain less
intensely than smaller "Newly formed" deposits. She also lists a Crystal Violet
method in the 3rd edition, but that it is more of a rapid s
I like to use a chart recorder on paraffin dispensers in case the thing goes
whacko over a weekend and boils the paraffin, then goes back to normal before
anyone notices.
Sent from my iPhone
> On Jan 8, 2015, at 6:10 PM, Elizabeth Chlipala wrote:
>
> All of our logs are use logs so we record
All of our logs are use logs so we record when we use any piece of equipment
and any QC or maintenance if required, we do not record or document anything if
we do not use it, other than lab temperature and humidity which we record daily.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Labo
Brain, we write in NIU for Not in Use if it is not used on a particular day.
However, you may want to do checks a couple times per week just to be sure it
is working so you don't find solidified paraffin the day you want to use it!
Since it takes just as much time to write NIU as it does to writ
, August 13, 2014 7:18 PM
> To: Walter Benton; Algeo, Lacie A; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: paraffin
>
> The nice thing about those is that "sticky mats" will also collect the dust
> from shoes; I found that these stop a lot of dust coming in
, Lacie A; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: paraffin
The nice thing about those is that "sticky mats" will also collect the dust
from shoes; I found that these stop a lot of dust coming in at the outside
door entrances, as well as having them to collect paraffin
western.edu
on behalf of Walter Benton
Sent: Wednesday, August 13, 2014 9:05 PM
To: Algeo, Lacie A; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: paraffin
You may want to purchase the large tack mats for staff to walk over when going
in and out of your embedding area. These come
You may want to purchase the large tack mats for staff to walk over when going
in and out of your embedding area. These come in blue, white, or gray:
http://www.fishersci.com/ecomm/servlet/itemdetail?storeId=10652&langId=-1&catalogId=29104&productId=5264212&distype=0&highlightProductsItemsFlag=Y&f
I am still trying to figure out what I need to do for validation for CAP. Do I
need to revalidate IHC and/or special stains if I change the paraffin on the
processor? The paraffin I have in mind is supposed to work faster than the one
we currently use for infiltration, so we may need to adjust
We use Richard Allen Type 6 paraffin for both infiltration and embedding (16
years) with no adverse effects. To validate you can get the pathologist to
give you samples for the same specimens you are running. IE; gallbladder 1
for patient dx and 1 for testing, uterus, appendix, any large spec
Phil
Are the slides prepared from the different paraffins being testing run on the
same H&E stain run or on different runs?
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premi
Sorry about the lack of Subject... I knew I was forgetting
something! ;)
Brendal Finlay, HT (ASCP)
-Original message-
From: Rene J Buesa rjbu...@yahoo.com
Date: Wed, 13 Feb 2013 12:09:08 -0600
To: Brendal Finlay brendal.fin...@medicalcenterclinic.com,
"histonet@lists.utsouthwestern.e
You could contact Simport. A few years ago I got some deeper disposable molds
from them and they work great with tissues that are too high for the regular
molds (like the Tissue-tek molds). The only problem is that they only came in
the large width and you waste a lot of paraffin. Another though
ssage-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liang, Frank
Sent: Thursday, January 19, 2012 8:40 AM
To: 'Pam Barker'; 'Histonet'
Subject: [Histonet] RE: paraffin embedding mold for rat eye
Hi, everyone,
Hi, everyone,
Any suggestions for the right size of mold to embed rat eyes in paraffin? Where
we can get them? The molds we currently use are good for mouse eyes.
Thanks,
Frank
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwes
Also, using 100% alcohol on the cold plate induces cracking. Freeze spray does
too, although some people will never admit to using it. Both of these things
induce the rapid cooling effect.
Toysha N. Mayer, MBA, HT (ASCP)
Instructor
Program in Histotechnology
School of Health Professions
MD Ander
Thanks Gayle for the tip, wouldn't hurt to improve. I will test it and post the
results.
THANK YOU,
PATTI RUBEN-NELSON H.T.(ASCP)
PNP LABORATORY CONSULTANTS
SUPERVISOR/DGC
P.O. BOX 412
CABAZON, CA. 92230
cell (909) 841-9761
nelsonr...@verizon.net
CONFIDENTIALITY NOTICE:
This message and
Hello, For DNA, we use a new blande and we wipe down microtome, handles, front
of blade holder with 95% ethanol on a gauze sponge. For RNA, use a new blade
and wipe down all with RNAzap including the water bath and rinse with DEPC
water. Use clean water in bath, distilled will work or DEPC wat
Hi Bret-
I've dealt with chondrocyte pellets a few times. It's a pretty half-assed
technique, but works pretty well if the pellet is relatively solid...if it's
not, maybe someone else can offer a useful suggestion :)
I was taught to use a piece of lens paper; pre-fold it in half several times,
another piece of fixed tissue , process it with standard paraffin
processing protocol.
Dr. Amita Dubey
PCSED,TRC
From:
John Kiernan
To:
"Thomas, Nancy"
Cc:
"histonet@lists.utsouthwestern.edu" ,
'An Eerdekens'
Date:
02/02/11 12:28 PM
Subject:
Re: [Histonet] RE:
You can't go from 70% alcohol into paraffin without passing through 100%
alcohol and then a clearing agent (liquid miscible with 100% alcohol and with
melted wax). Xylene is a commonly used clearing agent. Your times in 50% and
70% alcohol are much longer than necessary. Even for a whale's hypot
Hi An,
You did not mention a clearing step (xylene, or xylene substitute) between your
alcohol and paraffin steps. Maybe you just forgot to write it, but if you
actually missed this step, your samples would be improperly processed leading
to the problems you have.
Nancy Thomas
Stowers Institut
Hello to everyone,
Thank you for all the advice. I will reduce my processing time as well as
try to rehyrdate my paraffin blocks before cutting. I greatly appreciate the
help.
Thanks,
Michael
___
Histonet mailing list
Histonet@lists.utsouthwe
Hi Jeanine,
At the NSH vendors I got a sample of a neat clear plastic holder from Source
Medical Products, www.sourcemp.com, 1866-735-9965, holds 4 maybe 8 blocks for
mailing, depending upon the thickness.
Shirley
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Stephen
Kum Jew
Gesendet: Mittwoch, 03. März 2010 21:43
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Re: paraffin processing times for brain
Hi all
I was wondering if anyone can send
tonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: paraffin processing times for brain
Hi all
I was wondering if anyone can send me please the optimal paraffin
processing times for brain autopsy tissue.
Currently the blocks have cut and stained well but 4-5mm thick tissue
has expanded (bulged out) foll
Hi all
I was wondering if anyone can send me please the optimal paraffin
processing times for brain autopsy tissue.
Currently the blocks have cut and stained well but 4-5mm thick tissue
has expanded (bulged out) following cutting as if they had absorbed water.
Solutions been all chan
Allison we use tissue prep paraffin and it comes in cardboard containers, we
save the empty containers to pour the waste paraffin in. Once it solidifies we
dump it in the biohazardous container to be disposed of.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailt
We use a paraffin block dewaxer in our lab. It works great. I find it saves
time and doesn't create as much of a mess (with wax shavings). Some people in
our lab still prefer to trim the excess wax with a blade or forceps. I guess
it's all personal preference.
We coveted the ParaTrimmer for years, but could not afford to buy one. We
simply use a smaller size laboratory metal hot plate, propped it into a
metal pan so the hot plate is at an angle suitable to allow the melted
paraffin to run onto paper towels in the pan. This is one hot plate we
donate t
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Thursday, February 05, 2009 5:13 PM
To: Robert Richmond; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Paraffin Blocks
I've been prea
e of my 2
cents!!! j
From: Bonner, Janet [mailto:janet.bon...@flhosp.org]
Sent: Friday, February 06, 2009 8:50 AM
To: Weems, Joyce; Robert Richmond; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Paraffin Blocks
We are a very large facility and kept the majority of our bl
: [Histonet] Re: Paraffin Blocks
I've been preaching this sermon for several years!!! We must do
something!!! j
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Robert
Richmond
Sent: Thursday, Februa
tonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Paraffin Blocks
How long to retain paraffin blocks (human tissue) is an issue that's
going to need re-thinking.
Presently most pathology services in the USA retain them for no more
than ten years. If the laboratory closes, slides and blocks ar
How long to retain paraffin blocks (human tissue) is an issue that's
going to need re-thinking.
Presently most pathology services in the USA retain them for no more
than ten years. If the laboratory closes, slides and blocks are
disposed of immediately.
It seems to me that in the coming age of mo
: [Histonet] Re: Paraffin Blocks
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, February 4, 2009, 2:39 PM
I know this subject has probably been addressed before, but I need to poll the
net.
We are a Pathology Core in a research center. We have paraffin blocks (mostly
non-human) collected since we
@lists.utsouthwestern.edu
Subject: [Histonet] Re: Paraffin Blocks
I know this subject has probably been addressed before, but I need to poll
the
net.
We are a Pathology Core in a research center. We have paraffin blocks
(mostly
non-human) collected since we were established in 1986. Our core will soon
be
undergoing
I know this subject has probably been addressed before, but I need to poll the
net.
We are a Pathology Core in a research center. We have paraffin blocks (mostly
non-human) collected since we were established in 1986. Our core will soon be
undergoing renovation and we need to either store or di
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