[Histonet] Histonet] Freezing spray in cryostats

2019-09-26 Thread White, Lisa M. via Histonet
We did use freeze spray in the cryostat years ago. Then one fateful day we all had to go on protocol for exposure to TB because a resident sprayed a lung with the freeze spray and there you go. There is a turn around time for frozen sections we are all working with, HOWEVER it is not worth

Re: [Histonet] Freezing spray in cryostats

2019-09-25 Thread Akemi via Histonet
I make my tech’s wear PPE’s, goggles/masks, gloves and N95 masks for potential TB and regular masks for normal tissue. Akemi Allison Sent from my iPhone > On Sep 25, 2019, at 1:00 PM, Charles Riley via Histonet > wrote: > > I have to agree with Terri. I also believe that CAP is going to be

Re: [Histonet] Freezing spray in cryostats

2019-09-25 Thread Charles Riley via Histonet
I have to agree with Terri. I also believe that CAP is going to be putting in regulations forbidding the use of freeze sprays in cryostats soon (at least I think I heard a rumor about this). Anyway another useful item is liquid nitrogen if you really need a quick freeze. Dip a brush into the

Re: [Histonet] Freezing spray in cryostats

2019-09-25 Thread Terri Braud via Histonet
My question is why would you ever want to use an aerosol in the cryostat, taking a chance on aerosolizing some nasty bug? Is the 30 seconds you save in using freezing spray worth the exposure to TB or goodness-knows-what? We use a simple metal heat extractor (comes standard in our Leicas) and

[Histonet] freezing spray

2013-10-24 Thread Horn, Hazel V
Our hospital has not allowed freezing spray to be used in the frozen section lab for many years. We now have a new group of doctors who want to use the spray. The docs think the frozen sections take too long to freeze. Yet, they meet the frozen section TAT for more than 98% of our cases. I

Re: [Histonet] freezing spray

2013-10-24 Thread Jennifer MacDonald
spattering of droplets of infectious material.” From: Horn, Hazel V hor...@archildrens.org To: histonet (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Date: 10/24/2013 11:47 AM Subject:[Histonet] freezing spray Sent by:histonet-boun

[Histonet] Freezing spray artifact

2010-05-19 Thread Martin, Erin
Has anyone run into a problem or artifact from freezing spray? I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like. Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248

Re: [Histonet] Freezing spray artifact

2010-05-19 Thread Rena Fail
] Freezing spray artifact Has anyone run into a problem or artifact from freezing spray?  I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like. Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415

RE: [Histonet] Freezing spray artifact

2010-05-19 Thread Sebree Linda A
. Madison, WI 53792 (608)265-6596 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martin, Erin Sent: Wednesday, May 19, 2010 11:09 AM To: histonet Subject: [Histonet] Freezing spray artifact Has anyone run

RE: [Histonet] Freezing spray artifact

2010-05-19 Thread sgoebel
-51= 07 Original Message Subject: [Histonet] Freezing spray artifact From: Martin, Erin erin.mar...@ucsf.edu Date: Wed, May 19, 2010 9:09 am To: histonet histonet@lists.utsouthwestern.edu Has anyone run into a problem or artifact from freezing spray? I think we

Re: [Histonet] Freezing spray artifact

2010-05-19 Thread James L Burchette
. They are also good for soaking the face of a block and are better than a wad of tissue when wiping slides. JB Martin, Erin erin.mar...@ucsf.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 05/19/2010 12:41 PM To histonet histonet@lists.utsouthwestern.edu cc Subject [Histonet] Freezing spray

RE: [Histonet] Freezing spray artifact

2010-05-19 Thread Jennifer MacDonald
RE: [Histonet] Freezing spray artifact Erin, One can certainly get cracking of the tissue and surrounding paraffin from a too intense application of freezing spray. This is usually grossly visible, at least in the paraffin. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH