Hi Histopeeps,
How are you? I hope you are having a great week!
I have been engaged by several clients who are looking for the best of the
best in Immunohistochemistry.
These positions are all growth positions with leading edge state of the art
companies.
Do you have a passion for Immunohistochemis
For technologists, pathologists, residents, scientists or anyone else
interested in immunohistochemistry.
On Friday, October 25, 2019, the cIQc will be holding our 2019 Technical
Workshop: The Finer Details of IHC Readout. Click here to
[REGISTER](https://pathology-payment.med.ubc.ca/2019-
stant Director of Laboratory - Pathology
FH Moore Regional Hospital
910-715-5286 office
910715-1944 fax
-Original Message-
From: Robyn L. Vazquez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, February 09, 2017 7:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [His
L (ASCP)cm
Histology Manager
(509)892-2744
mgio...@incdx.com
-Original Message-
From: Robyn L. Vazquez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, February 09, 2017 4:42 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Immunohistochemistry ghosting
Hello,
We have been experiencing problems with ghosting (inconsistent and/or no
staining) mainly affecting our control area which is located at the bottom of
the slide. Random antibodies markers are being affected. We were using Fisher
Scientific then TruBond slides. We are using Ventana Ultras
Hi Everyone:
What is the designation of someone who is certified (registered) in
immunohistochemistry?
Thanks,
Tim Wheelock
Harvard Brain Bank
Mclean Hospital
Belmont, MA
The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sen
The best source for such a beginner (and for some "seasoned" as well) is the
DAKO manual.
René J.
From: Candice Smoots
To: Histonet
Sent: Monday, November 18, 2013 11:42 AM
Subject: [Histonet] Immunohistochemistry for beginners
Hi fellow histo
gt; Pam Marcum
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots
> Sent: Monday, November 18, 2013 10:42 AM
> To: Histonet
> Subject: [Histonet] Immunohistochemistry
A
Sent: Monday, November 18, 2013 11:49 AM
To: 'Candice Smoots'; Histonet
Subject: RE: [Histonet] Immunohistochemistry for beginners
Dako has some excellent training information for beginners and I think Leica
may also have something. If you talk to your Dako rep or contact them by
Subject: [Histonet] Immunohistochemistry for beginners
Hi fellow histonetters
I have a colleague that is completely new to immunohistochemistry. I have gone
over what I could with him, however, I think that some literature would help
him more. Do you guys know of any books or articles that could help
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Candice Smoots
Sent: Monday, November 18, 2013 10:42 AM
To: Histonet
Subject: [Histonet] Immunohistochemistry for beginners
Hi fellow histonetters
I have a colleague that is completely new to
Hi fellow histonetters
I have a colleague that is completely new to immunohistochemistry. I have gone
over what I could with him, however, I think that some literature would help
him more. Do you guys know of any books or articles that could help a beginner
like him. I do not have any on hand b
Skip the buffer and go in the hematox straight from water. See if that
works.
JB
On Aug 1, 2013 3:20 PM, "Tarantelli, Rebecca Anne" wrote:
> Hi all,
>
> I am new to this forum, but a friend recommended I send my issue out to
> you for help. Four weeks ago I completed a new protocol for
> Immunohi
...@gmx.at]
Sent: Friday, August 02, 2013 7:40 AM
To: Tarantelli, Rebecca Anne
Cc: histonet@lists.utsouthwestern.edu
Subject: AW: [Histonet] immunohistochemistry help please
Are all slides from the same tissue sample with the same pretreatment?
I can't believe, that you don't have any hematoxyli
t 2013 21:20
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] immunohistochemistry help please
Hi all,
I am new to this forum, but a friend recommended I send my issue out to you
for help. Four weeks ago I completed a new protocol for Immunohistochemistry
(IHC) using protinase K epitope
ursday, August 1, 2013 3:20 PM
Subject: [Histonet] immunohistochemistry help please
Hi all,
I am new to this forum, but a friend recommended I send my issue out to you for
help. Four weeks ago I completed a new protocol for Immunohistochemistry (IHC)
using protinase K epitope retrieval, a
Hi all,
I am new to this forum, but a friend recommended I send my issue out to you for
help. Four weeks ago I completed a new protocol for Immunohistochemistry (IHC)
using protinase K epitope retrieval, and monoclonal antibody 3F6. It worked
perfectly. Three days later, after 13 beautiful stai
Juli 2013 23:36
An: 'histonet@lists.utsouthwestern.edu'
Betreff: [Histonet] Immunohistochemistry TAT
Histonetters,
I am looking for some help in getting IHC slides out in an 8 hour period
of time. Here is some information.
* We have a busy Immunohistochemistry lab and the volume of
requests
Histonetters,
I am looking for some help in getting IHC slides out in an 8 hour period of
time. Here is some information.
* We have a busy Immunohistochemistry lab and the volume of requests
keep increasing. We offer approximately 150 different IHC stains ( including
ISH and Dua
Hello All
I am a clinical researcher so my questions may be somewhat naive, but I would
be extremely grateful for any help.
I want to look at adipose tissue macrophage polarity by immunohistochemistry
for activation markers. I have the following questions:
1. How much tissue sample is requi
Hello everyone,
Melissa here with ASP. Just an update to let you all know that I have an
Immunohistochemistry Supervisor position in New York. Message me for more
information. Thank you as always for letting us post our job openings on
here!
To view a complete list of Allied Search Partners curre
To everyone in histoland that is familiar with IHC,
I am a new histologist, unfamiliar with many techniques in IHC. Here in my
lab, we section devices that contain cells, membrane and scaffolding.
Unfortunately, due to the scaffolding, the devices do not hold up well when
embedded in paraffin. Lik
Allied Search Partners has been retained in the search for a qualified IHC
Technician/Technologist for a position in East Texas. The candidate should
be a highly professional, client/patient-focused individual for a
challenging and rewarding full-time career. Work in an environment where
leading-
Hello,
I'm new to the histo listserv. I'm having problems with stain TLR4 on paraffin
embedded mouse tissue. Does anyone have experience with this particular immuno
stain? Please provide the company name and catalog number.
Thanks,
Bella Shmaltsuyev
__
treet
> Suite 100
> Austin, Texas 78744
> (512)901-0900 ext. 6912
>
>
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos
> Brooks
> Sent: Tuesday, September 13, 2011 3
-boun...@lists.utsouthwestern.edu] On Behalf Of
sdys...@mirnarx.com
Sent: Tuesday, September 13, 2011 1:36 PM
To: amosbro...@gmail.com; histonet@lists.utsouthwestern.edu;
wim.vandenbro...@ugent.be
Subject: RE: [Histonet] immunohistochemistry: manual staining
I tried ordering this system a few months
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos
Brooks
Sent: Tuesday, September 13, 2011 3:34 PM
To: histonet@lists.utsouthwestern.edu; wim.vandenbro...@ugent.be
Subject: [Histonet] immunohistochemistry: manual staining
Hi,
Shandon Sequenza staining racks and slide clips
laces the reagents below.
It is great for manual IHC. Drop me an email if you want some more info.
Amos
On Tue, Sep 13, 2011 at 10:06 AM, wrote:
> Message: 12
> Date: Tue, 13 Sep 2011 14:08:53 +0200
> From: "Prof. dr. Wim Van den Broeck"
> Subject: [Histonet] immunohisto
, OK 73139
405-759-3953 Lab
405-759-7513 Fax
www.excaliburpathology.com
From: John Shelley
To: "wim.vandenbro...@ugent.be" ;
"histonet@lists.utsouthwestern.edu"
Sent: Tue, September 13, 2011 8:05:45 AM
Subject: RE: [Histonet] immunohistochemis
Sent: Tuesday, September 13, 2011 8:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] immunohistochemistry: manual staining
Dear Colleagues,
We do a lot of immunohistochemical stainings (research, no diagnostics), but
unfortunately, we cannot afford an automated stainer. At the moment
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Prof. dr. Wim
Van den Broeck
Sent: Tuesday, September 13, 2011 7:09 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] immunohistochemistry: manual staining
Dear Colleagues,
We do a lot of
Dear Colleagues,
We do a lot of immunohistochemical stainings (research, no diagnostics), but
unfortunately, we cannot afford an automated stainer. At the moment, we do
everything manually (washing, incubating, ) but I would like to make
things run more smoothly. So my question is, is there an
Jennell,
The 2007 article from Applied immunohistochemistry & molecular
morphology "Recommendations for Improved Standardization of
Immunohistochemistry" by Goldstein et.al. is the article we used to
setup our validation protocols. It is a very good idea to get a head
start on a thorough validatio
Morning,
We have ours in the oven at 65 for a minimum of 30mins. We are also using the
DAKO PTlink system. It works great for us
Matt Lunetta HT (ASCP)
Longmont United Hospital
Message: 2
Date: Mon, 31 Jan 2011 06:30:55 -0500
From: "Kuhnla, Melissa"
Subject: RE: [Histonet] Immunohist
...@aol.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Immunohistochemistry validation
I just received a copy of HISTOLOGIC, December 2010, which is provided by
Sakura. The cover article is titled "Antibody Optimization and Validation".
-Original Message-
From
Behalf Of
jcarpenter...@aol.com
Sent: Tuesday, February 01, 2011 11:06 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Immunohistochemistry validation
Does anyone have any good advice or good articles I can read about validating
immuno stains?
thank
Does anyone have any good advice or good articles I can read about validating
immuno stains?
thanks. Jennell
=
___
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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Hi Milton,
We've begun baking ALL IHC slides for 2 hours at 60 degrees C. This also
applies to unstained extra slides that may potentially used for IHC, including
extra sections on breast cores, lymph node cores, cytology cell blocks etc. No
specific room temperature drying time before the o
30, 2011 9:01 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Immunohistochemistry slides drying and baking
protocol
Hello Histonetters,
How and for how long and at what temperature is everyone air drying and
baking slides for IHC staining?; specially for breast and cytol
Hello Histonetters,
How and for how long and at what temperature is everyone air drying and baking
slides for IHC staining?; specially for breast and cytology slides.
Thank you very much in advance,
MG
This email message, including
B Inc
--
Message: 4
Date: Fri, 21 Jan 2011 09:41:39 -0500
From: Jan Berry
Subject: [Histonet] Immunohistochemistry in plastic sections
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset=us-ascii
I'm wondering if anyone has exper
I'm wondering if anyone has experience with immunohistochemistry using plastic
sections. I am currently working with 4um sections, and have tried doing
staining with and without Dako antigen retrieval, but I am not getting very
good results. Tissues (mostly mouse bones, but some soft tissues a
de= Dr. Bldg 4 Suite 100
Austin, Texas 78744
(= 512)386-5107
Original Message
Subject: RE: [Histonet] Immunohistochemistry images
From: "Blazek, Linda" <[1]lbla...@digestivespecialists.com>
Date: Thu, October 14, 2010 9:04 am
To: 'Mic
@lists.utsouthwestern.edu
Subject: [Histonet] Immunohistochemistry images
Hi everyone, I was wondering if anyone knew of a website that I can view and
save the IHC images. We are putting together a new procedure manual and the
pathogists want images of the antibodies we use to be included in the manual.
Thanks in
Try IHCWORLD Image Gallery.
http://www.ihcworld.com/imagegallery/
Jan Shivers
UMN VDL
- Original Message -
From: "Michele Carr"
To:
Sent: Thursday, October 14, 2010 10:40 AM
Subject: [Histonet] Immunohistochemistry images
Hi everyone, I was wondering if anyone knew of
Hi everyone, I was wondering if anyone knew of a website that I can view and
save the IHC images. We are putting together a new procedure manual and the
pathogists want images of the antibodies we use to be included in the manual.
Thanks in advance for your responses.
Michele Carr HTL ASCP
Medi
Has anyone has any experience using immunohistochemistry with the
following fixation?
Polyethylene glycol (PEG) solution: 25% glycol, 10% ethanol (95%), 10%
formaldehyde (37%), and 55% distilled water.
Specifically if there are any enzymatic reactions that I should
avoid/block for/etc.
Thanks for
We have an opening in our IHC Lab - contact HR or myself with questions.
ProPath, a progressive, CAP accredited, high-volume pathology lab in Dallas
has a full-time opportunity for an Immunohistochemistry Technician.
Responsibilities include: slide preparation (paraffin and frozen sections)
--
Date: Thu, 4 Jun 2009 20:01:50 -0700 (PDT)
From: Hatem Salim
Subject: [Histonet] immunohistochemistry
To: histonet@lists.utsouthwestern.edu
HI
I am Research assistant at Physiology department of Michigan state
university. I am using the (ß-Catenin Antibody (Carboxy-terminal Antig
gayle callis
Sent: Friday, June 05, 2009 3:28 PM
To: 'Troutman, Kenneth A'; 'Histonet'
Subject: RE: [Histonet] immunohistochemistry
Good advice since actively boiling buffer puts mechanical stresses on
section, with bone more easily loosened by this action. It may help to
prehe
I second Mr. Troutman's recommendation using silane slides, = but air
dry overnight. Once the slides have been baked and paraffin re= moved,
place the slides in citrate buffer and heat them in a closed plastic
coplin jar for 12 hours at 75 degrees C. Some epitopes can be
Subject: Re: [Histonet] immunohistochemistry
I would recommend the following:
Citrate for 35 min at 95 deg. Cool down for 10 min. (This is our
protocol.)
Be sure you are using charged slides and I would let them air dry for at
least 1 hour before heating and deparaffinizing. That might help
com/main/> <http://www.vanderbilt.edu/>
<http://www.mc.vanderbilt.edu/>
Date: Thu, 4 Jun 2009 20:01:50 -0700 (PDT)
From: Hatem Salim
Subject: [Histonet] immunohistochemistry
To: histonet@lists.utsouthwestern.edu
Message-ID: <782588.78944...@web46103.mail.sp1.yahoo.com>
Content-
Good luck!
-Original Message-
From: Hatem Salim
Date: Thu, 04 Jun 2009 20:01:50
To:
Subject: [Histonet] immunohistochemistry
HI
I am Research assistant at Physiology department of Michigan state university.
I am using the (ß-Catenin Antibody (Carboxy-terminal Antigen) #9587 for IH
HI
I am Research assistant at Physiology department of Michigan state university.
I am using the (ß-Catenin Antibody (Carboxy-terminal Antigen) #9587 for IHC on
mice femur sections. I have used this antigen unmasking method: For Citrate:
Bring slides to a boil in 10 mM sodium citrate buffer pH
I am working in immuno Lab doing hgh volume and i need resource where i can
have some continuing education.Thanks if any one can help.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/
2009 09:51:39
To:
Subject: [Histonet] immunohistochemistry with CD markers
Hello!
Can anyone help us with CD markers? We want to use some CD markers on mouse
testis, and the antibodies data sheets explain that only freeze samples,
cryosectioned and post-fixed with acetone can be used. We t
Hello!
Can anyone help us with CD markers? We want to use some CD markers on mouse
testis, and the antibodies data sheets explain that only freeze samples,
cryosectioned and post-fixed with acetone can be used. We tried to do this
protocols, but without any result. We frozen the sample with
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