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Subject: [Histonet] Xylene substitutes for clearing agents
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Hello histonetters
From: Hagon, Christopher (Health) via Histonet
Sent: Tuesday, August 27, 2019 10:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes for clearing agents
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Hello histonetters!
I realise that this has been asked a lot, but cannot find a good link for the
comparisons of each. I am charged with looking into converting our lab to go
xylene free. We don't want to go down the limonene path, so that leaves the
isopropyl alcohol method, or
You wrote:
Hello Histo Land,
Does anyone use xylene substitutes in their stain line only? I am especially
interested in d-limonene options. Thanks in advance!
*
We
Hello Histo Land,
Does anyone use xylene substitutes in their stain line only? I am
especially interested in d-limonene options. Thanks in advance!
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We used NOTHING in our staining line. We dewaxed with 2% dishwasher soap
followed by the normal staining (no xylene → no ethanol).After staining we
dried the stained section in an oven at 60ºC for 15 minutes → coverslip (no
ethanol → no xylene).To process we used iso-propanol → mineral oil →
I know this question has been asked before but what's the best and safest
Xylene Substitute?
Ruth Yaskovich
N.I.H. N.I.D.C.R.
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that produce
optimal quality slides.
Try it!
René J.
From: Yaskovich, Ruth A (NIH/NIDCR) [E] ryaskov...@dir.nidcr.nih.gov
To: 'Histonet@lists.utsouthwestern.edu' Histonet@lists.utsouthwestern.edu
Sent: Wednesday, August 21, 2013 11:18 AM
Subject: [Histonet] Xylene
at= tachment, is
prohibited.
Original M= essage
Subject: Re: [Histonet] Xylene Substitutes
From= : Rene J Buesa [2]rjbu...@yahoo.com
Date: Wed, August 21, 2013 12:06 pm
To:Yaskovich,Ruth=A(NIH/NIDCR)[E][3]rya
skov
...@dir.nidcr.nih.gov; 'Histonet@lists.utsouthwestern.edu'
Histonet@lists.utsouthwestern.edu
Sent: Wednesday, August 21, 2013 12:20 PM
Subject: RE: [Histonet] Xylene Substitutes
Rene,
How do you recommend cover slipping with out xylene? We use a tape cover slip
instrument that requires xylene. If you
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene substitutes for tissue processor clean cycle
I use a excelsior and was told to always use Xylene in the cleaning cycle.
Since sub is not as pure as xylene ( obviously) you could be at risk of
developing paraffin clogs
Hello all,
The technician who does preventive maintenance on our LX-120 tissue
processor recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue in the clean cycle and would
appreciate any recommendations.
Thanks very much,
Carla Conway
21, 2013 11:25 AM
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle
Hello all,
The technician who does preventive maintenance on our LX-120 tissue
processor recommended that we use xylene as the solvent in our clean cycle.
We are considering using a xylene substitue
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Tuesday, May 21, 2013 9:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle
11:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle
Hello all,
The technician who does preventive maintenance on our LX-120 tissue processor
recommended that we use xylene as the solvent in our clean cycle.
We are considering using
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Tuesday, May 21, 2013 11:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes for tissue processor clean cycle
Hello all,
The technician who does preventive maintenance on our LX-120 tissue
.
V.Avalos
ADS, INC
Fax:602-277-2134
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Conway, Carla
Sent: Tuesday, May 21, 2013 8:26 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene substitutes
...@lmhealth.org
www.LMHealth.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes
ext. 5197
janine.simmsco...@jmmc.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes
Hello all,
My lab is looking
...@lists.utsouthwestern.edu] On Behalf Of Adrienne
Anderson
Sent: Wednesday, February 06, 2013 6:30 AM
To: Tom McNemar
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Xylene Substitutes
Thanks so much, everyone, for the feedback.
I have a colleague who is convinced paint thinner is exactly
: RE: [Histonet] Xylene Substitutes
Not familiar with SubX but we have used Americlear for many years with good
results. You just can't beat Xylene for some things though and still keep a
little around.
Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Wednesday, February 6, 2013 8:30 AM
Subject: Re: [Histonet] Xylene Substitutes
Thanks so much, everyone, for the feedback.
I have a colleague who is convinced paint thinner is exactly the same as the
SubX we use. Does anyone
Adrienne,
If you'd like information on Formula 83 please contact me off list at
b...@cbgbiotech.com
Thanks,
Beth Sell
CBG
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-Original Message-
From: Adrienne Anderson [mailto:rennie1...@yahoo.com]
Sent: Tuesday, February 05, 2013 4:58 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Xylene Substitutes
Hello all,
My lab is looking
Of Dessoye,
Michael J
Sent: Wednesday, February 06, 2013 11:47 AM
To: Adrienne Anderson; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Xylene Substitutes
We used SubX but have switched to Clear-Rite 3 from Thermo Fisher with
excellent results as well as cost savings.
Michael J. Dessoye
;
histonet@lists.utsouthwestern.eduhistonet@lists.utsouthwestern.edu
Sent: Wednesday, February 6, 2013 5:36 AM
Subject: RE: [Histonet] Xylene Substitutes
Not familiar with SubX but we have used Americlear for many years with good
results. You just can't beat Xylene for some things though and still keep
Hello all,
My lab is looking into xylene substitutes, and I'd love some feedback on what
other labs are using. We currently use SubX, but are there other items out
there more economical?
Thanks,
Adrienne
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Liz,
You are correct. If you bring in an FDA-approved test with NO
modifications, all that is required is verification; you simply have to
verify that the test works in your lab when you follow the recommended
procedure. Validation, which is required for laboratory-developed tests
as well as
Vanessa,
I work with a lot of great histology folks and what I know about it is that
eosin bleeding and blue-haze problems usually occur as a consequence of
either not fully removing all of the water during the final absolute alcohol
rinse step, and/or not fully removing all of the alcohol during
What are labs using for xylene substitute. I will be using it in a hand
staining setup for Mohs. Feed back on Histo-Clear, Clear solve, S-3
and formular 83.
Thanks in advance.
Dawn Oakes HT
-
Confidentiality Notice:
We use Formula 83 and are very happy with it. We use it exclusively in the
VIP processor and to deparaffinize before staining. We also use as clearing
agent after staining, prior to coverslipping, but have found that we still
need the last container before coverslipping to be xylene for best
We use formula 83 for everything and do not have a problem with a film. We
have found that we must use isopropal alcohol instead of reagent alcohol in the
final dehydration steps.
Margaret Perry
SDSU
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My hand staining setup contained some commercial naphta mix, which is
comparable to any other petroleum benzine solvant, for dewaxing.
For dehydrating/coverslipping, I used n-Butyl acetate a.k.a. butyl
ethanoate. Coverslipping can be done with any xylene soluble glue.
What are labs using for
We use Formula 83 ... we have had no problems in all phases.
Gary
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
njoydo...@aol.com
Sent: Wednesday, May 13, 2009 7:25 AM
To:
; Histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] xylene substitutes
We use Formula 83 ... we have had no problems in all phases.
Gary
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
njoydo...@aol.com
Sent
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