Re: [spctools-discuss] Variable mod query in COMET

2023-07-31 Thread Debojyoti Pal
I meant that they would come up with something to get it done with only variable modifications. By updating the code. Not by the static + variable workaround we are doing. Thanks Debojyoti On Tue, Aug 1, 2023 at 10:14 AM 'Luis Mendoza' via spctools-discuss < spctools-discuss@googlegroups.com>

Re: [spctools-discuss] Variable mod query in COMET

2023-07-31 Thread 'Luis Mendoza' via spctools-discuss
This should already be possible with, e.g. variable_mod02 = 40.000 C 0 3 -1 0 0 0.0 add_C_cysteine = 50.000 (Of course using whatever masses are appropriate.) Or does this not work? On Mon, Jul 31, 2023 at 9:31 PM Debojyoti Pal wrote: > Thanks Luis. I have asked this on the COMET group as

Re: [spctools-discuss] Variable mod query in COMET

2023-07-31 Thread Debojyoti Pal
Thanks Luis. I have asked this on the COMET group as well. They will probably come up with an update or something to enable it. On Tuesday, August 1, 2023 at 8:47:46 AM UTC+5:30 Luis Mendoza wrote: > Hi Debojyoti, > Yes, I would say this is the most straightforward way of conducting this >

Re: [spctools-discuss] Variable mod query in COMET

2023-07-31 Thread 'Luis Mendoza' via spctools-discuss
Hi Debojyoti, Yes, I would say this is the most straightforward way of conducting this search. Note that you may need to make further adjustments to the variable mod string settings if, say, a given peptide must have *all* of its Cysteines in one state (+50) or the other (+90). The default is to

[spctools-discuss] Variable mod query in COMET

2023-07-31 Thread Debojyoti Pal
Hello everyone I have a specific scenario where I want to search for peptide in which cysteine must either have +50 or +90 mod. Basically: 1) Cys can only have one of +50 or +90 2) Cys much have one of +50 or +90 (ie It cannot be unmodified) Only way I can achieve this is to put a static mod of