Well - you must have 3-4 molecules in the asymmetric unit. Does the wild type form a dimer? trimer? etc And what is the wild type cell and spacegroup? MR should work I think.. Eleanor
Jenny wrote:
*** For details on how to be removed from this list visit the *** *** CCP4 home page http://www.ccp4.ac.uk *** Hi, All I got a set of data of my crystal recently(2.3 A) and tried to do MR because it's only several residues different from the wild type. The problem is that after we indexed with HK:2000, it shows the primitive rhombohedral space group, with the cell size 137.200 137.200 86.682 90.000 90.000 120.000. The protein is only 90 residues ( 10kDa), so looks to me the cell size is a little big.But anyway, we tried to use the wt structure and do MR, didn't find the solution for the space group R32.We also tried C centered monoclinic space group and primitive triclinic space group, didn't get any luck either.Since the crystal is grown from the preticipate, so I started to worry that maybe the crystal isn't my protein.After I checked with SDS gel of the crystal, it shows very similar MW as the target protein.Now I'm waiting for the sequencing result. I heard that it's quite tricky for R32 and I wondered what's that.If the sequence comes back to be correct, what should I do the next step?If MR doesn't succeed, does this mean, the new mutant protein is very different from the wild type? Any suggestions would be appreciated. Thanks. Jenny
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