Hi Daniel, You can also refer to HATODAS, http://hatodas.harima.riken.go.jp/, where you can find suitable heavy atom compounds to use based not only on the sequence of your protein but also the crystallization conditions, as is your case. This is based on the following paper: Heavy-atom Database System: a tool for the preparation of heavy-atom derivatives of protein crystals based on amino-acid sequence and crystallization conditions. Acta D, Volume 61 Part 9 , 1302-1305 Sept. 2005 Regards, Debanu.
________________________________ From: CCP4 bulletin board on behalf of Daniel Gutmann Sent: Tue 4/17/2007 10:03 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Hey there, I have crystallised a protein in 30% MPD and it it gives nice native data. However, it seems impossible to get heavy metal derivatives and I read that MPD does chelate heavy metals. Did any of you make similar experiences with xtals in MPD conditions? If so did you find a good solution (introduction of selmet makes the protein ustable) to make heavy metal derivatives? Best Regards Daniel
