We use a Beckman Coulter DU730. It has a small footprint if lab space is an issue. It will do single-wavelength, multi-wavelength, or take an entire spectrum in 0.5 nm steps if you desire. It comes with the standard 1 ml cuvette holder, but we also purchased the microcuvette accessory for volumes in the 100 uL range. We've been using the instrument for over a year now with no problems. Results for determining protein concentrations using the method of Pace et al * *Protein Sci.<javascript:AL_get(this,%20'jour',%20'Protein%20Sci.');>1995 Nov;4(11):2411-23 are very reproducible using this spec.
On Thu, Dec 4, 2008 at 10:37 AM, James M. Vergis <[EMAIL PROTECTED]>wrote: > I would also recommend the nanodrop. It takes a whole spectra every > measurement and there is no need to dilute your sample. You can demo it > for > a week and try it out. > > > ==================================================== > James M. Vergis, Ph.D. > University of Virginia Molecular Physiology and Biological Physics > MKWEINR 360A Snyder Building > 480 Ray C. Hunt Drive > PO Box 800886 > Charlottesville, VA 22908-0886 > phone: 434-243-2730 FAX: 434-243-8271 > [EMAIL PROTECTED] > ==================================================== > > > -----Original Message----- > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Tim > Gruene > Sent: Thursday, December 04, 2008 10:16 AM > To: CCP4BB@JISCMAIL.AC.UK > Subject: [ccp4bb] suggestions for UV spectrometer > > Dear all, > > we would like to purchase a UV spectrometer for measuring protein > concentrations (280nm), and I would like to here your comments and > especially recommendations. > > We don't need anything fancy, a small, fast device would be sufficient. > > Tim > > > -- > Tim Gruene > Institut fuer anorganische Chemie > Tammannstr. 4 > D-37077 Goettingen > > GPG Key ID = A46BEE1A > -- Jim Fairman Graduate Research Assistant Department of Biochemistry, Cellular, and Molecular Biology (BCMB) University of Tennessee -- Knoxville 216-368-3337 [EMAIL PROTECTED] [EMAIL PROTECTED]
