CCP4 bulletin board <CCP4BB@JISCMAIL.AC.UK> wrote on 12/04/2008 10:16:02 AM:
> Dear all, > > we would like to purchase a UV spectrometer for measuring protein > concentrations (280nm), and I would like to here your comments and > especially recommendations. > > We don't need anything fancy, a small, fast device would be sufficient. > > Tim > > Hi Tim - I'm going to add my voice to the chorus for the Nanodrop. It's small - the size of a Kleenex box (plus a computer to run it). It's fast - you'll have a reading within two minutes of walking up to the bench, and that includes cleaning, initializing, and blanking the instrument. You can probably process 2-3 samples a minute if you're efficient since the actual measurement takes only a few seconds. There are no quartz cuvettes to wash, indeed no cuvettes at all - you just pipet 2 ul of your sample onto the measurement pedestal. Cleanup is a breeze - just wipe with a damp Kimwipe and you're done. The light source (xenon flash lamp) doesn't need to warm up and I've never had to change the bulb. The software is efficient and simple - you can do a table of measurements at a single wavelength, or full spectra in whatever wavelength range you want (from 190-720 nm). It measures 1 mm and 0.1 mm path lengths then combines the spectra, so you can use a broad range of sample concentrations without dilution - very useful for 50 mg/ml crystallization stocks! It can also do more sophisticated analysis, such as analyzing dye labeling efficiency by deconvoluting the dye absorbance spectrum from the protein absorbance spectrum. If you get one, you'll never want to go back to the old way. (And no, they didn't pay me to say this!) - Matt -- Matthew Franklin , Ph.D. Senior Scientist, ImClone Systems 180 Varick Street, 6th floor New York, NY 10014 phone:(917)606-4116 fax:(212)645-2054 Confidentiality Note: This e-mail, and any attachment to it, contains privileged and confidential information intended only for the use of the individual(s) or entity named on the e-mail. If the reader of this e-mail is not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that reading it is strictly prohibited. If you have received this e-mail in error, please immediately return it to the sender and delete it from your system. Thank you.
