At the risk of dragging this discussion even further afield from
crystallography:
How can you get realistic numbers for concentrated solutions using
the Nanodrop? I understand that the instrument reduces absorbance by
using a very short path length. However, I thought that in order for
the Beer-Lambert formalism to be applicable, the solution needs to be
sufficiently dilute so that the chance of molecules "shadowing" one
another is negligible. Isn't this condition violated for concentrated
solutions (even with short path lengths)?
Pat
On 4 Dec 2008, at 1:27 PM, Michael Giffin wrote:
We also like the Nanodrop...
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Patrick J. Loll, Ph. D.
Professor of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA
(215) 762-7706
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