Re: [Histonet] Paraffin Block triming
Hi Scott, We have one from Thermo Fisher (Shandon)and love it. It saves time and fingers in cleaning cassettes. I also use it to trim and shape large blocks for sectioning. No shavings no razor blades. Pam Marcum - Original Message - From: Scott lsc...@sfcn.org To: Histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 12:19:45 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Automating muscle and neuro stains
I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc. Would your lab use this technology? Would you do this work in house instead of sending it out if it were automated? How many tests does your lab do per year in this category? Would you prefer this was a feature on your automated special stainer? I will compile the answers. Thanks in advance for your participation. Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC Technical Director Massachusetts General Hospital _ Express your personality in color! Preview and select themes for Hotmail®. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX_WL_HM_express_032009#colortheme___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Block trimming
I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:akbitt...@geisinger.edu N:Bitting;Angela END:VCARD ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Block trimming
You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block René J. --- On Thu, 3/19/09, Angela Bitting akbitt...@geisinger.edu wrote: From: Angela Bitting akbitt...@geisinger.edu Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, Jennifer MacDonald jmacdon...@mtsac.edu, Scott lsc...@sfcn.org Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Block triming
Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 19, 2009 6:31 AM To: 'Angela Bitting'; histonet-boun...@lists.utsouthwestern.edu; 'Jennifer MacDonald'; 'Scott' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this message including all attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
In my experience the paraffin block trimmer is quicker and easier on your hands than the old manual scraping method. Loralee McMahon, HTL (ASCP) ICC Supervisor University of Rochester Department of Pathology (585) 275-7210 From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Thu 3/19/2009 9:25 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; Angela Bitting Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block René J. --- On Thu, 3/19/09, Angela Bitting akbitt...@geisinger.edu wrote: From: Angela Bitting akbitt...@geisinger.edu Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, Jennifer MacDonald jmacdon...@mtsac.edu, Scott lsc...@sfcn.org Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin Block triming
I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Block trimming
My techs use our Para Trimmer from Thermo Scientific Shandon. It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 --- On Thu, 3/19/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, Jennifer MacDonald jmacdon...@mtsac.edu, Scott lsc...@sfcn.org, Angela Bitting akbitt...@geisinger.edu Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:25 AM You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block René J. --- On Thu, 3/19/09, Angela Bitting akbitt...@geisinger.edu wrote: From: Angela Bitting akbitt...@geisinger.edu Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, Jennifer MacDonald jmacdon...@mtsac.edu, Scott lsc...@sfcn.org Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Block trimming
I agree that this is quicker than manually---you can do 4?or 5 blocks at a time. -Original Message- From: Karin Groeger kgroe...@uslabs.net To: Pamela Marcum pmar...@vet.upenn.edu; Angela Bitting akbitt...@geisinger.edu; histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald jmacdon...@mtsac.edu; Scott lsc...@sfcn.org Cc: Histonet@lists.utsouthwestern.edu Sent: Thu, 19 Mar 2009 9:37 am Subject: RE: [Histonet] Paraffin Block trimming They work great for us and saves the hand. We use the Shandon Para Trimmer. Our techs love them. Karin Groeger Histology Supervisor US LABS, Irvine,CA 949-450-0145 ext. 649 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, March 19, 2009 6:31 AM To: 'Angela Bitting'; histonet-boun...@lists.utsouthwestern.edu; 'Jennifer MacDonald'; 'Scott' Cc: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Actually the warm up time is about 3 minutes and after that I can do blocks much faster and cleaner than scraping. I can't imagine it being slower to be honest. We love it as it save a lot of time. Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, March 19, 2009 9:14 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anyb ody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This message, including any attachments, may contain CONFIDENTIAL AND/OR LEGALLY PRIVILEGED information. The information is intended for use by the individual named above and may not be disseminated to any other party without US LABS' written permission. If you are not the intended recipient, or the employee or agent responsible for delivering this information to the intended recipient, you are hereby notified that any dissemination, disclosure, distribution, copying or taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this information in error, please notify US LABS immediately at 1-888-450-0145 attn: Compliance Department to arrange for return of this me ssage including all attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
We have one timmer. I don't use it, I hand trim simply because I am quicker with it. The other techs use the timmer to save their hands. Tom Podawiltz, HT (ASCP) Histology Section Head/Laboratory Safety Officer LRGHealthcare 603-524-3211 ext: 3220 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd [kdboydhi...@yahoo.com] Sent: Thursday, March 19, 2009 10:03 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott; Angela Bitting; rjbu...@yahoo.com Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming My techs use our Para Trimmer from Thermo Scientific Shandon. It will save your hands, but it takes a lot more time than the old fashion hand scraping. I prefer to hand scrape. I can scrape at least twice as many by hand than with the para trimmer. Kelly D. Boyd, BS, HTL (ASCP) Lab Manager Harris Histology Services 2025 Eastgate Dr. Ste. F Greenville, NC 27858 www.harrishisto.com Tele (252)-830-6866 Cell (252)-943-9527 Fax (252)-830-0032 --- On Thu, 3/19/09, Rene J Buesa rjbu...@yahoo.com wrote: From: Rene J Buesa rjbu...@yahoo.com Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, Jennifer MacDonald jmacdon...@mtsac.edu, Scott lsc...@sfcn.org, Angela Bitting akbitt...@geisinger.edu Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:25 AM You will have to time worself as I did to find out that waiting fo the paraffin to melt in order to eliminate it takes 2.5 times more time than doing it manually with a pocket knife (as I used). Manually = 32 secs/block vs. 80 secks./block René J. --- On Thu, 3/19/09, Angela Bitting akbitt...@geisinger.edu wrote: From: Angela Bitting akbitt...@geisinger.edu Subject: Re: [Histonet] Paraffin Block trimming To: histonet-boun...@lists.utsouthwestern.edu, Jennifer MacDonald jmacdon...@mtsac.edu, Scott lsc...@sfcn.org Cc: Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 9:13 AM I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily
RE: [Histonet] Paraffin Block trimming
No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
FW: [Histonet] RE: Automating muscle and neuro stains
Have I died and gone to histologists heaven? Automate an ATPase, now there would be something special. No more crying and wailing over failed or partially successful acid reversal. I'll have to retreat to a darkened room, I'm traumatised. Ian. Dr. Ian Montgomery, Histotechnology, I.B.L.S. Support Unit, Thomson Building, University of Glasgow, Glasgow, G12 8QQ. Subject: RE: [Histonet] RE: Automating muscle and neuro stains I was hoping to determine the level of interest in having muscle and neuro stains automated? I am speaking with a vendor about the desire to automate many of my stains: ATP, NADH, SDH, COX, Acetyl cholinesterase, Luxol Fast Blue, etc. Would your lab use this technology? Would you do this work in house instead of sending it out if it were automated? How many tests does your lab do per year in this category? Would you prefer this was a feature on your automated special stainer? I will compile the answers. Thanks in advance for your participation. Denise Bland-Piontek, CTBS(AATB)HTL(ASCP)QIHC Technical Director Massachusetts General Hospital _ Express your personality in color! Preview and select themes for HotmailR. http://www.windowslive-hotmail.com/LearnMore/personalize.aspx?ocid=TXT_MSGTX _WL_HM_express_032009#colortheme ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 Martin, Gary gmar...@marshallmedical.org wrote: No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
Don't know what to say.I fill mine up and as long as I don't get to rough with it (bump it or something where it sloshes) I don't have any excess paraffin around the edges. I use metal base molds. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Merced Leiker [mailto:lei...@buffalo.edu] Sent: Thursday, March 19, 2009 10:56 AM To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 Martin, Gary gmar...@marshallmedical.org wrote: No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Sanderson's RBS Image
Does anyone have a picture of what a slide stained w/Sanderson's Rapid Bone should look like? No, but Google does. About a half a second of searching found these... http://www.surgipath.com/products/itm_photos/p111d26-large.jpg http://archotol.ama-assn.org/cgi/reprint/129/10/1125.pdf Suzanne Bruce wrote: Hi, Does anyone have a picture of what a slide stained w/Sanderson's Rapid Bone should look like? Thanks in advance, Suzanne ___ Suzanne Bruce, R.V.T. Histologist Necropsy Coordinator https://69.61.197.115/exchweb/bin/redir.asp?URL=http://www.vetpathservicesinc.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block triming
I am also one of that -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Block trimming
The lab I work at part time uses the new paraffin trimmer by Surgipath, it is alot bigger than the one I have seen before in other labs. You can do approx. 8 blocks at a time. But I also agree, if you clean embedd, you do not have all that extra paraffin to clean up. Kathy Hicks H.T. (ASCP) DPNS Surgical Center 400 SKokie Blvd. Suite 450 Northbrook, Illinois 60062 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin block - cassette cleaner
Hello, We have the Thermo Fisher model as well and love it. It does an excellent job on the blocks.in much less time than traditional hand scraping. Karen J Kay, MLT Pathology Supervisor,Chinook Health Laboratory Chinook Regional Hospital,Lethbridge, Alberta, CANADA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: March 19, 2009 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. equipment purchasing (Patty Dunlop) 2. glycerol buffered antifade media (Neil M. Fournier) 3. Double-stain for immune cell markers (Margaryan, Naira) 4. Paraffin Block triming (Scott) 5. Re: Paraffin Block trimming (Jennifer MacDonald) 6. Re: Paraffin Block triming (Pamela Marcum) 7. RE: RE: Automating muscle and neuro stains (Denise Piontek) 8. Re: Paraffin Block trimming (Angela Bitting) 9. Re: Paraffin Block trimming (Rene J Buesa) 10. RE: Paraffin Block trimming (Pamela Marcum) 11. Re: Paraffin Block triming (Peter Carroll) 12. RE: Paraffin Block trimming (Karin Groeger) 13. RE: Paraffin Block trimming (McMahon, Loralee A) 14. Paraffin Block triming (Paula Pierce) 15. RE: Paraffin Block triming (Bartlett, Jeanine (CDC/CCID/NCZVED)) 16. Re: Paraffin Block trimming (Kelly Boyd) 17. Re: Paraffin Block trimming (godsgal...@aol.com) 18. RE: Paraffin Block trimming (Podawiltz, Thomas) 19. RE: Paraffin Block trimming (Martin, Gary) 20. FW: [Histonet] RE: Automating muscle and neuro stains (Ian Montgomery) 21. Sanderson's RBS Image (Suzanne Bruce) 22. RE: Paraffin Block trimming (Merced Leiker) -- Message: 1 Date: Wed, 18 Mar 2009 10:09:40 -0700 From: Patty Dunlop pdunlop...@gmail.com Subject: [Histonet] equipment purchasing To: histonet@lists.utsouthwestern.edu Message-ID: 80ab7bc60903181009o6e178432m42613f7e5cc0e...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hello, Our histo lab is currently looking into buying one or all of the following: cassette labeler, automated stainer (that can also accommodate a few special stains), and automated coverslipper (that uses minimal xylene or no xylene). Can anyone recommend good brands and models that they like as well as what vendors might have good prices? We are also looking into getting the stainer and coverslipper either used or refurbished to cut down on costs. Our lab is small and we do only about 40-50 slides per day on average. We would prefer to have the equipment to be as small as possible. Any suggestions are welcome! Thanks, Patty -- Message: 2 Date: Wed, 18 Mar 2009 13:18:23 -0600 From: Neil M. Fournier nfourn...@sasktel.net Subject: [Histonet] glycerol buffered antifade media To: histonet@lists.utsouthwestern.edu Message-ID: e742d69a2175489792ebe1cff973c...@neil45eaf11e9e Content-Type: text/plain; charset=us-ascii Hi Everyone, I have to make up antifade media. The recipe I was given is: 5 ml 0.1 M phosphate buffer, 50 mg p-phenylenediamine, 45 ml glycerol (pH to ~ 10.0 with NaOH) However, I notice that solution became quite a dark yellow when dissolving. Is this normal? It seems to occur every time I try to make it up. Thanks in advance Neil E-mail message checked by Spyware Doctor (6.0.0.386) Database version: 5.11990 http://www.pctools.com/en/spyware-doctor-antivirus/ -- Message: 3 Date: Wed, 18 Mar 2009 14:20:34 -0500 From: Margaryan, Naira nmargar...@childrensmemorial.org Subject: [Histonet] Double-stain for immune cell markers To: histonet@lists.utsouthwestern.edu Message-ID: 6a2230bac92e3b4084dae06869b89fb601e6c...@cmhexc01evs.childrensmemorial.org Content-Type: text/plain; charset=us-ascii Hi Histofriends, I was requested to make the double-staining for immune cell markers together with another genes (IgG goat) that our lab researching on human tumors xenografted in mouse. Which companies kits you will suggest to use for this experiment (the double-staining) and, what Abs (not mouse and not goat) are the best to represent the immune cell markers. Respectfully, Naira -- Message: 4 Date: Wed, 18 Mar 2009 22:19:45 -0600 From: Scott
RE: [Histonet] Paraffin Block trimming
hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) --On Thursday, March 19, 2009 10:57 AM -0400 Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov wrote: Don't know what to say.I fill mine up and as long as I don't get to rough with it (bump it or something where it sloshes) I don't have any excess paraffin around the edges. I use metal base molds. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Merced Leiker [mailto:lei...@buffalo.edu] Sent: Thursday, March 19, 2009 10:56 AM To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 Martin, Gary gmar...@marshallmedical.org wrote: No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin Block trimming
I don't normally embed or cut my own sections anymore, but when I was doing it a few years ago on a larger scale (but still in a research setting), clean embedding generally did the trick. But when I did need to remove the excess wax on the sides of the cassette, I would melt it using a hotplate. In fact I nearly always did this during the embedding process. Once the blocks had solidified I would go back and melt the wax on the sides using the hot plate of the embedding station. Only if needed, on an individual block basis, would I scrape with a straight edge blade at the time of sectioning. -Original Message- From: Merced Leiker lei...@buffalo.edu Date: Thu, 19 Mar 2009 10:55:36 To: Martin, Garygmar...@marshallmedical.org; Bartlett, Jeanine (CDC/CCID/NCZVED)j...@cdc.gov; Paula Piercecont...@excaliburpathology.com; Histonethistonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paraffin Block trimming Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 Martin, Gary gmar...@marshallmedical.org wrote: No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Conversion of histo lab to Lean Sigma
Hi histo-netters, Our lab is trying to go Lean and I was wondering if anyone out there has done the conversion yet? Do you have any pointers or suggestions on how to make the transition easier. Our Department is in operation from 7am to 6:30pm. Thanks and have a great day This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] tissue processors/UK
Best low volume tissue processor, microwave or conventional, mainly lung biopsies in the short term, any ideas chaps??, thanks. Cheers Richard Edwards Leicester University U.K. P.S. happy to hear from vendors. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] dako autostainers
We are looking into buying the Dako Autostainer Plus. I asked our rep if they still have any older models around to look into. He made it sound like there are older models but, the stainer itself has stayed the same, just the software has changed/improved. He said they don't even like selling the older models anymore b/c they are worried that the software would be out of date. I looked into this on Dako.com and found that there is the older Dado Autostainer. After reading through its description it looks like it just lacks a few of the bells and whistles (like a slide labeler for example) that the Autostainer Plus has but, otherwise looks like it gets the job done just the same. Does anyone have any feedback on either one of these machines and whether or not you think I should even bother looking into the older model? Thank you so much. Jen C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block triming
Of course Shakunyou trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Shakun Aswani [mailto:shakun.asw...@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
We have used a variety of cassettes and paraffin over the years...same result. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Merced Leiker [mailto:lei...@buffalo.edu] Sent: Thursday, March 19, 2009 11:16 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) --On Thursday, March 19, 2009 10:57 AM -0400 Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov wrote: Don't know what to say.I fill mine up and as long as I don't get to rough with it (bump it or something where it sloshes) I don't have any excess paraffin around the edges. I use metal base molds. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Merced Leiker [mailto:lei...@buffalo.edu] Sent: Thursday, March 19, 2009 10:56 AM To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 Martin, Gary gmar...@marshallmedical.org wrote: No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] processing v-e-r-y tiny samples
Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block triming
Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -Original Message- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:j...@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakunyou trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Shakun Aswani [mailto:shakun.asw...@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
I guess one has to go to histology school to learn how to do it, then (sent under separate cover from another histonetter...) I 'm too researchy. ;-) --On Thursday, March 19, 2009 11:45 AM -0400 Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov wrote: We have used a variety of cassettes and paraffin over the years...same result. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Merced Leiker [mailto:lei...@buffalo.edu] Sent: Thursday, March 19, 2009 11:16 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) --On Thursday, March 19, 2009 10:57 AM -0400 Bartlett, Jeanine (CDC/CCID/NCZVED) j...@cdc.gov wrote: Don't know what to say.I fill mine up and as long as I don't get to rough with it (bump it or something where it sloshes) I don't have any excess paraffin around the edges. I use metal base molds. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Merced Leiker [mailto:lei...@buffalo.edu] Sent: Thursday, March 19, 2009 10:56 AM To: Martin, Gary; Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming Now I'm interested in how you embed so as to not have to scrape...if i don't add enough wax (enough to end up rising around the edges of the cassette, hence the scraping later), i don't get a secure hold of the block to the cassette... ML --On Thursday, March 19, 2009 7:37 AM -0700 Martin, Gary gmar...@marshallmedical.org wrote: No you're not the only one ... I was wondering the same thing ... why all the scraping. It seems to me that clean embedding does the trick with a few exceptions. G -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this
[Histonet] PSLIM slide printer
I think mine was probably the last lab to purchase a PSLIM from AccuPlace before they sold off the printer to Fisher. Just in time! For what it is worth, I do like the printer. It is great for a small lab and is saving what is left of my wrist. However, we are on the second machine. The first one wasted about as many slides as I accepted. We called tech support and tried their suggestions but nothing worked. After we sent them a picture of what the slides looked like they called with an RMA number and sent out a replacement that is working perfectly. I'm happy! We had an option of purchasing a service/replacement agreement with the printer and I'm glad we did. I don't know if Fisher is offering this option but if you are considering the printer it might be a good idea. Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block triming
I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds cold at room temperature and don't have much leakage whereas techs who keep the molds hot have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -Original Message- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:j...@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakunyou trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Shakun Aswani [mailto:shakun.asw...@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Sandersons rapid bone stain photo
Maybe the NSH hard tissue committee has a picture. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of gayle callis Sent: Thursday, March 19, 2009 11:39 AM To: 'Histonet' Subject: [Histonet] RE: Sandersons rapid bone stain photo On looking at the Surgipath photo, the bone is very pale although the osteocytes, osteoid and soft tissues look good. I am not sure what counterstain (two are touted in the stain data sheet) was used as seen in this photo. One is a basic fuchsin that turns the bone red. Be careful of overstaining with fuchsin counterstain. The other is light or fast green and since we never used that, I can't comment on the color contrast with bone and soft tissue components. This may be the stain used in photograph??? It always is a good idea to look at the stain while performing the actual staining method. After prescribed or desired time in RBS, simply rinse quickly with running hot tap water, blot and examine under the microscope to see depth of stain for soft tissue/bone components. To view a dry section, simply place a coverglass on top of section for higher power examination. If components stained by RBS not dark enough, you can return to the hot RBS. Rinse then blot, and counterstain after that. It does not hurt the bone section to dry. In fact we preferred a dry section when going back into either stain just to prevent dilution of the stain solution, particularly when doing hand ground thick section surface staining. Gayle Callis HTL(ASCP)HT,MT Bozeman MT 59715 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block triming
Exactly right... And you can melt your blocks on the embedding center. The Thermo Paratrimmer is great tho. J Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 678-843-7376 - Phone 678-843-7831 - Fax -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Doug Showers Sent: Thursday, March 19, 2009 12:37 PM To: Histonet Subject: RE: [Histonet] Paraffin Block triming I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds cold at room temperature and don't have much leakage whereas techs who keep the molds hot have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -Original Message- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:j...@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakunyou trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Shakun Aswani [mailto:shakun.asw...@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message.
RE: [Histonet] Paraffin Block triming
I disagree. I take my molds right out of the holding chamber and use warm and have hardly any leakage and don't have to hardly scrape. However, the other techs do have leakage and have to scrape alot off. I think it is just in the embedders technique. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Doug Showers Sent: Thursday, March 19, 2009 11:37 AM To: Histonet Subject: RE: [Histonet] Paraffin Block triming I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds cold at room temperature and don't have much leakage whereas techs who keep the molds hot have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -Original Message- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:j...@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakunyou trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Shakun Aswani [mailto:shakun.asw...@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the
RE: [Histonet] Paraffin Block triming
I also use mine warm Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab Sent: Thursday, March 19, 2009 1:03 PM To: 'Doug Showers'; Histonet Subject: RE: [Histonet] Paraffin Block triming I disagree. I take my molds right out of the holding chamber and use warm and have hardly any leakage and don't have to hardly scrape. However, the other techs do have leakage and have to scrape alot off. I think it is just in the embedders technique. Dawn -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Doug Showers Sent: Thursday, March 19, 2009 11:37 AM To: Histonet Subject: RE: [Histonet] Paraffin Block triming I think the amount of excess paraffin depends on the temperature of the mold when you are embedding. I like my molds cold at room temperature and don't have much leakage whereas techs who keep the molds hot have a higher amount of leakage around the edges. Doug Showers, MS, HT Histology Manager ProPath 8267 Elmbrook Dr. Suite 100 Dallas, TX 75247 To learn more about ProPath, please visit http://www.ProPathLab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shakun Aswani Sent: Thursday, March 19, 2009 11:03 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Jeanine, We had the best Chief instructor Billie Swisher. I am so very thankful that I got the training from that school. I do miss you guys Shakun -Original Message- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:j...@cdc.gov] Sent: Thursday, March 19, 2009 8:45 AM To: Shakun Aswani; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming Of course Shakunyou trained me! Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: Shakun Aswani [mailto:shakun.asw...@acologix.com] Sent: Thursday, March 19, 2009 11:09 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I am also one of that -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/CCID/NCZVED) Sent: Thursday, March 19, 2009 6:58 AM To: Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block triming I was wondering if I was the only one out there that rarely has to scrape a block. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce Sent: Thursday, March 19, 2009 9:57 AM To: Histonet Subject: [Histonet] Paraffin Block triming I try to embed so as to have a minimal amount of paraffin to scrape from the blocks. ;) But, I do scrape using the handle end of the same forceps I use to pick up the ribbon and tease the sections. No sharp edge. No electricity. PKP From: Peter Carroll carro...@umdnj.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 8:30:34 AM Subject: Re: [Histonet] Paraffin Block triming Does anybody use a paraffin block dewaxer ? Yep, it's called my own two hands and a metal spatula, ha ha :) I find that it's not only very quick, but quite accurate... Scott wrote: Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged
Re: [Histonet] PSLIM slide printer
Andi, How are you utilizing the printer? We are trying to incorporate the printer with real time printing as the tech scans the bar coded cassette. We have had one tech working with the PSLIM and they have printed about 200 slides over a couple of days, but it has been getting progressively worse in performance.If there is more than one slide needed for the block then the PSLIM can hang. It's as if it doesn't have enough memory. We are on our second or third unit and are about to give up and go to labels. The PSLIM was questionable at $5K, but out of the question at Fisher's $10+K pricing. The print quality is so much better than the Leica. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 vic...@pathology.washington.edu 206-598-2792 206-598-7659 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. Andrea Grantham wrote: I think mine was probably the last lab to purchase a PSLIM from AccuPlace before they sold off the printer to Fisher. Just in time! For what it is worth, I do like the printer. It is great for a small lab and is saving what is left of my wrist. However, we are on the second machine. The first one wasted about as many slides as I accepted. We called tech support and tried their suggestions but nothing worked. After we sent them a picture of what the slides looked like they called with an RMA number and sent out a replacement that is working perfectly. I'm happy! We had an option of purchasing a service/replacement agreement with the printer and I'm glad we did. I don't know if Fisher is offering this option but if you are considering the printer it might be a good idea. Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mosquito GI Tract
And here I thought I had an unusual project today, finding out as I did that my boss is thinking of examining up to 1000 cattle for TB (at 10 lymph nodes/beast) using AFB and asking me to cost it for him. I think the Mosquito GI Tract Processing and Embedding question has my project beat! How would you even KNOW you had the darned GI tract to begin with? Are these Texas Mosquitos? I'm just sayin'... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Paraffin Block triming
I think, it depends on the amount of paraffin you fill in the mold, before you put the cassette on it. If it is a little too much, you press the paraffin out. Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
I've noticed that if I put a little extra pressure on the cassette on top of the mold when putting in the liquid paraffin it doesn't seem to seep out the bottom sides and therefore cleaner blocks. The fill level in the cassette matters too. I usually fill mine about 3/4 to the top of the cassette to compensate for shrinkage during chilling and still have the block and cassette firmly mounted together. We use regular fisherbrand cassettes and metal molds. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Merced Leiker Sent: Thu 3/19/2009 10:15 AM To: Bartlett, Jeanine (CDC/CCID/NCZVED); Martin, Gary; Paula Pierce; Histonet Subject: RE: [Histonet] Paraffin Block trimming hmmm...I do too...Fisher 15-182-505B HistoPrep Stainless-Steel Base Molds...maybe it's the cassette design or type of wax used? (VWR 15147-839 POLYFIN, VWR 87002-362 HISTOSETTE Cassettes, Simport Plastics Biopsy Cassettes) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:cassette labeler
Our techs and pathologists love our Leica cassette and slide printers. We've had them for over 2 years now, with few problems. The cassette labeler holds up to 6 different colors, and has an additional manual feed for anything special. The slide holds up to 3 different slide types, and also has a manual feed. Its my understanding that Sakura uses the exact same hardware. They are great time savers and very easy to use. Be sure to always order the optional unload station. It helps keep order. We are looking forward to interfacing the slide printer with CoPath next year. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3874 fax - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block triming
I use the minimum amount in the base mold then top it off after I add the cassette top. Jeanine Bartlett Infectious Diseases Pathology Branch (404) 639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Thursday, March 19, 2009 2:40 PM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Paraffin Block triming I think, it depends on the amount of paraffin you fill in the mold, before you put the cassette on it. If it is a little too much, you press the paraffin out. Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 64, Issue 33
Hi Patty and all Histoneters, I am an independent histology equipment repair technician, based in the Atlanta, GA. Sometimes I acquire used equipment for resale and right now I have: 1) Leica Autostainer XL 1) Leica CV 5000 cover slipper Others can give testament to the quality and reliability of Leica instruments. (with the possible exception of their tissue processors). I invite you to respond either on or off of Histonet. References available upon request. Best regards, Paul Firnschild QA Support Services Inc. 404.291.3715 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 10:56 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 64, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] dehydration
In an emergency situation (like not enough hrs in a day) - how long can tissue remain in the final ETOH before going into the paraffin embedding stage? I do all my work manually - no automation here!! Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mosquito GI Tract
lol...I wanted to say the same thing...and how in the world can you even see it when you section it? How do you stain it without losing it from the slide? Is there really enough surface area for it to adhere and withstand washing? ...and it's ALMOST Friday...! --On Thursday, March 19, 2009 12:09 PM -0600 Breeden, Sara sbree...@nmda.nmsu.edu wrote: And here I thought I had an unusual project today, finding out as I did that my boss is thinking of examining up to 1000 cattle for TB (at 10 lymph nodes/beast) using AFB and asking me to cost it for him. I think the Mosquito GI Tract Processing and Embedding question has my project beat! How would you even KNOW you had the darned GI tract to begin with? Are these Texas Mosquitos? I'm just sayin'... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mosquito GI Tract
More interesting - why is someone even looking at the mosquito GI tract? What happens in there that we need to know about? I guess for all the diseases those filthy beasts carry - they must harbor viruses and bacteria somewhere. Did you know that mosquitos did not exist in Hawaii until a little more than 100 years ago? Durn Westerners brought them in the barrels of drinking water they carried on their ships. Welcome to paradise. Oh - they also brought measles. Thanks a lot. Merced Leiker lei...@buffalo.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 03/19/2009 02:11 PM To Breeden, Sara sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Mosquito GI Tract lol...I wanted to say the same thing...and how in the world can you even see it when you section it? How do you stain it without losing it from the slide? Is there really enough surface area for it to adhere and withstand washing? ...and it's ALMOST Friday...! --On Thursday, March 19, 2009 12:09 PM -0600 Breeden, Sara sbree...@nmda.nmsu.edu wrote: And here I thought I had an unusual project today, finding out as I did that my boss is thinking of examining up to 1000 cattle for TB (at 10 lymph nodes/beast) using AFB and asking me to cost it for him. I think the Mosquito GI Tract Processing and Embedding question has my project beat! How would you even KNOW you had the darned GI tract to begin with? Are these Texas Mosquitos? I'm just sayin'... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fluoro Jade Query
What's the latest and greatest on Fluoro Jade for degenerating neurons? I've found a few references, but they are old - - so am I - just celebrated my birthday - - - - anyway - -who's got the best answer? Winner gets a Hershey Bar! Jackie ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] dehydration
It seems that you are missing the clearing step or antemedium in the sequence you describe but, the tissue should not stay too long in neither alcohol (unless it is 2-propanol) or in the antemedium (unless it is mineral oil) in which cases the time in them is not an issue. René J. --- On Thu, 3/19/09, Dianne Holmes dhol...@anatomy.umsmed.edu wrote: From: Dianne Holmes dhol...@anatomy.umsmed.edu Subject: [Histonet] dehydration To: Histonet Histonet@lists.utsouthwestern.edu Date: Thursday, March 19, 2009, 2:54 PM In an emergency situation (like not enough hrs in a day) - how long can tissue remain in the final ETOH before going into the paraffin embedding stage? I do all my work manually - no automation here!! Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Mosquito GI Tract
Actually I had a project to find bacteria in the ovaries of drosophila. The bacteria looked a lot like mitochondria and about the same size. That was a lot of fun, but at least I got to use a TEM for the imaging. You could barely see the sample in the dissecting microscope and it was only after I had post fixed it with osmium tetroxide and they turned black. And they wanted longitudinal sections too! That was fun ;-| On 3/19/09 3:27 PM, Jackie M O'Connor Jackie.O'con...@abbott.com wrote: More interesting - why is someone even looking at the mosquito GI tract? What happens in there that we need to know about? I guess for all the diseases those filthy beasts carry - they must harbor viruses and bacteria somewhere. Did you know that mosquitos did not exist in Hawaii until a little more than 100 years ago? Durn Westerners brought them in the barrels of drinking water they carried on their ships. Welcome to paradise. Oh - they also brought measles. Thanks a lot. Merced Leiker lei...@buffalo.edu Sent by: histonet-boun...@lists.utsouthwestern.edu 03/19/2009 02:11 PM To Breeden, Sara sbree...@nmda.nmsu.edu, histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Mosquito GI Tract lol...I wanted to say the same thing...and how in the world can you even see it when you section it? How do you stain it without losing it from the slide? Is there really enough surface area for it to adhere and withstand washing? ...and it's ALMOST Friday...! --On Thursday, March 19, 2009 12:09 PM -0600 Breeden, Sara sbree...@nmda.nmsu.edu wrote: And here I thought I had an unusual project today, finding out as I did that my boss is thinking of examining up to 1000 cattle for TB (at 10 lymph nodes/beast) using AFB and asking me to cost it for him. I think the Mosquito GI Tract Processing and Embedding question has my project beat! How would you even KNOW you had the darned GI tract to begin with? Are these Texas Mosquitos? I'm just sayin'... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Merced M Leiker Research Technician II 354 Biomedical Research Building School of Medicine and Biomedical Sciences State University of New York at Buffalo 3435 Main St, Buffalo, NY 14214 Ph: (716) 829-6033 Fx: (716) 829-2725 No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sanderson's Rapid Bone Stain
Dear Fellow Histonetters, There are 3 counterstains that can be used with the Sanderson's Rapid Bone Stain (RBS). We primarily used the acidified acid fuchsin, however, we had clients that only used a van Gieson counterstain. Light green can also be used. We normally stained for 1 minute ground sections in the RBS, rinsed in water and then counterstained. As Gayle mentioned, intensity of the RBS or the counterstain can be increased with addtional time. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Double-stain for immune cell markers
Hi Naira, Try Biocare. They make fantastic reagents for research as well as some great double-stain kits. As for the antibodies, it looks like you might have to create these either as cocktails (a lot of front-end work) or just make your stain longer (a lot of overall work) by staining seprately. Abcam has some esoteric reagents that you might be able to use for some non-mainstream species like horse or rat or guinea pig. Also I did not see which markers you are planning to stain for (there are quite a few immune cell markers). I might be able to help you find good reagents if I had that information. Good luck. Ashley Troutman BS, HT(ASCP)QIHC Histopathology Laboratory Department of Pathology Vanderbilt University Medical Center Nashville, TN http://www.vanderbilthealth.com/main/ http://www.vanderbilt.edu/ http://www.mc.vanderbilt.edu/ http://www.vanderbilthealth.com/main/ Message: 3 Date: Wed, 18 Mar 2009 14:20:34 -0500 From: Margaryan, Naira nmargar...@childrensmemorial.org Subject: [Histonet] Double-stain for immune cell markers To: histonet@lists.utsouthwestern.edu Message-ID: 6a2230bac92e3b4084dae06869b89fb601e6c...@cmhexc01evs.childrensmemorial.org Content-Type: text/plain; charset=us-ascii Hi Histofriends, I was requested to make the double-staining for immune cell markers together with another genes (IgG goat) that our lab researching on human tumors xenografted in mouse. Which companies kits you will suggest to use for this experiment (the double-staining) and, what Abs (not mouse and not goat) are the best to represent the immune cell markers. Respectfully, Naira ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE:Sanderson's RBS image
Thanks Gayle, I also looked at the Surgipath image, my slide was much more brilliant then that one. I did use the acid fuchsin counter stain. I just wasn't sure if mine would be considered 'over stained.' I'll send you an image that Jack Ratliff sent me that compares to what I was seeing today. I'm not using GMA, but Technovit 7200 ~100 micron thick ground sections. Suzanne Date: Thu, 19 Mar 2009 10:38:51 -0600 From: gayle callis gayle.cal...@bresnan.net Subject: [Histonet] RE: Sandersons rapid bone stain photo To: 'Histonet' histonet@lists.utsouthwestern.edu Message-ID: 000401c9a8b1$30e43630$92aca2...@callis@bresnan.net Content-Type: text/plain; charset=us-ascii On looking at the Surgipath photo, the bone is very pale although the osteocytes, osteoid and soft tissues look good. I am not sure what counterstain (two are touted in the stain data sheet) was used as seen in this photo. One is a basic fuchsin that turns the bone red. Be careful of overstaining with fuchsin counterstain. The other is light or fast green and since we never used that, I can't comment on the color contrast with bone and soft tissue components. This may be the stain used in photograph??? It always is a good idea to look at the stain while performing the actual staining method. After prescribed or desired time in RBS, simply rinse quickly with running hot tap water, blot and examine under the microscope to see depth of stain for soft tissue/bone components. To view a dry section, simply place a coverglass on top of section for higher power examination. If components stained by RBS not dark enough, you can return to the hot RBS. Rinse then blot, and counterstain after that. It does not hurt the bone section to dry. In fact we preferred a dry section when going back into either stain just to prevent dilution of the stain solution, particularly when doing hand ground thick section surface staining. Gayle Callis HTL(ASCP)HT,MT Bozeman MT 59715 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] processing v-e-r-y tiny samples
Andi only gets the fun stuff!!! Pamela A Marcum University of Pennsylvania School of Veterinary Medicine Comparative Orthopedic Laboratory (CORL) 382 W Street Rd Kennett Square PA 19438 610-925-6278 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bryan Watson Sent: Thursday, March 19, 2009 4:39 PM To: histonet@lists.utsouthwestern.edu; Andrea Grantham Subject: Re: [Histonet] processing v-e-r-y tiny samples I may never complain about tiny GI or bronch biopsies ever again! Andrea Grantham algra...@u.arizona.edu 3/19/2009 11:51 AM Good Morning! In keeping with the weirdness of the projects I get in this lab today my question is about processing mosquito GI tracts. I have a processing schedule - that is not the problem. I'm wondering if anybody out in histoland has a suggestion for what kind of cassette to use. I was thinking of the histoscreen cassette because these GI tracts are so thin (I think thinner than a hair)and I don't want to wrap them or use sponges because I'm afraid that I'll loose them or crush them. Any ideas? Andi . : Andrea Grantham, HT(ASCP) Dept. of Cell Biology Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415)Tucson, AZ 85724-5044USA : : (FAX: 520-626-2097) (email: algra...@u.arizona.edu) : :...: http://www.cba.arizona.edu/histology-lab.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: processing v-e-r-y tiny samples
Andi, We process E7.0 mouse embryos and have problems sometimes because they are so very tiny and fragile. We've wrapped them and sometimes (but not all the time) had them break and flatten. Usually we use the histoscreen cassettes. They still will sometimes break apart using these but we have our best luck using them. You might find, though, that even with the mesh, the diameter of the holes may be big enough to let the sample pass through if the gut samples are as small as you say. We tried the cell saver mesh inserts and they were a disaster. Lost the sample in them. I won't use them for our embryo work. As for the histogel or agar, we've had difficulty using either in our paraffin processing. Recent histonet emails reveal other folks having a problem with the stuff randomly getting hard and brittle, like plastic and ruining the sample for sectioning. We may have 4 or 5 blocks, all go through the processor together, and one might have the problem of turning brittle. According to the other emails, Richard Allan folks hadn't any clue as to what was happening or how to fix it. And I'm clueless as well. Wish I knew how to consistently process things without this happening. Pre-embedding in a matrix like this would be so very helpful to allowing us to manipulate and properly orient tiny, fragile samples. Good luck, and let us know what you come up with! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Free- Floating Frozen Brain Sections
Hi: I am new to this Forum and also to Immunohistochemistry of frozen sections. I want to use Floating Frozen brain sections to do some of immunohisto and immunofluorescence study. I will be grateful to you if you could provide me detail protocol for free floating brain sections. I meant fixation, cryopreservation, if want to store the tissue which cryoprotectant to be used and how to proceed further for staining and treating with antibodies. At least for now I want to use Hypoxyprobe, a marker for Hypoxia. and Vascular marker CD31 along with Neuronal and astrocyte marker. I Guess its going to be Multilabeling. Also i would like to have information on multilabeling. your suggestion on doing 4 markers at a time in one tissue. thanks -- Shah. Kaushik K ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Re: processing v-e-r-y tiny samples
Our cytolab produces cytoblocks with plasma, that they bring to coagulation. The coagel is a smooth droplet in the size of 5-10 mm diameter. I could imagine, that the GI can be caught in such a coagel, and then be placed in a mesh-cassette. Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] free Tissue Tek II vacuum infiltrator
I have an old Tissue Tek II vacuum infiltrator free to a good home. Only cost will be shipping via ground..either FedEx or UPS. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] list of special stains
Does anyone have or know where to obtain a list of special stains that briefly describes which each one is for? Thanks, Patricia Zerfas National Institutes of Health Building 28A, Room 112 28 Library Drive Bethesda, MD 20892 ph: (301) 496-4464 fax: (301) 402-1068 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Sanderson's Rapid Bone Stain
One other counterstain can be used also. Alizarin Red S for the calcium as long as acid etching is not performed on the surface of thick sections. I would presume von Kossa could be used also if Alizarin Red S has been done in past. Gayle Callis -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cathy Mayton Sent: Thursday, March 19, 2009 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sanderson's Rapid Bone Stain Dear Fellow Histonetters, There are 3 counterstains that can be used with the Sanderson's Rapid Bone Stain (RBS). We primarily used the acidified acid fuchsin, however, we had clients that only used a van Gieson counterstain. Light green can also be used. We normally stained for 1 minute ground sections in the RBS, rinsed in water and then counterstained. As Gayle mentioned, intensity of the RBS or the counterstain can be increased with addtional time. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block triming
We also have a paratrimmer and agree with Pam Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: Thursday, 19 March 2009 9:35 PM To: Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block triming Hi Scott, We have one from Thermo Fisher (Shandon)and love it. It saves time and fingers in cleaning cassettes. I also use it to trim and shape large blocks for sectioning. No shavings no razor blades. Pam Marcum - Original Message - From: Scott lsc...@sfcn.org To: Histonet@lists.utsouthwestern.edu Sent: Thursday, March 19, 2009 12:19:45 AM GMT -05:00 US/Canada Eastern Subject: [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Paraffin Block trimming
No, We find them faster and we believe safer Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, 20 March 2009 12:14 AM To: histonet-boun...@lists.utsouthwestern.edu; Jennifer MacDonald; Scott Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Paraffin Block trimming I've been told that they are slower than scraping by hand. What's the consensus? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! Jennifer MacDonald jmacdon...@mtsac.edu 3/19/2009 12:26 AM We have two in our student lab. It is safer, quicker, and there are a lot less paraffin shavings to clean up. Jennifer MacDonald Scott lsc...@sfcn.org Sent by: histonet-boun...@lists.utsouthwestern.edu 03/18/2009 09:24 PM To Histonet@lists.utsouthwestern.edu cc Subject [Histonet] Paraffin Block triming Hi, Does anybody use a paraffin block dewaxer ? If so does it save any time, how well does it work? Thanks, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] van Giesons for Sanderson's Rapid Bone Stain can be Unna's modified VG
Unna's modified van Gieson's stain for the Rapid Bone Stain is reported to give deeper colors than the VG recipe/solution commonly found in histotechnology textbooks. It contains a higher concentration of dyes but also contains nitric acid which is going to acid etch the surface of the bone a bit. The resulting counterstain will have deeper colors. Be aware that commercial solutions of van Giesons might give weaker staining. Gayle Callis HTL(ASCP)HT,MT Bozeman MT 59715 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cathy Mayton Sent: Thursday, March 19, 2009 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sanderson's Rapid Bone Stain Dear Fellow Histonetters, There are 3 counterstains that can be used with the Sanderson's Rapid Bone Stain (RBS). We primarily used the acidified acid fuchsin, however, we had clients that only used a van Gieson counterstain. Light green can also be used. We normally stained for 1 minute ground sections in the RBS, rinsed in water and then counterstained. As Gayle mentioned, intensity of the RBS or the counterstain can be increased with addtional time. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraffin Block triming Thanks for the info
Well thanks for all of the input. We have always scraped the blocks with an empty cold basemold, and it works fine. I had recieved ane-mail advertisment from one of our suppliers, it had a picture of the TBS brand Shur Trim block trimmer. I was just thinking it might save a little time, and mess. We use the Simport cassettes with a metal lid. I have found that if I dont have enough paraffin to scrape a little off the sides, it usually doesn't have enough to securly hold the specimen. Sometimes that causes sectioning issues. So I over do it a little to save myself the trouble of re-embedding. Just one of my quirks I guess. Thanks again, Scott Hendricksen HT (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
