[Histonet] 5 Yr old Leica ST5020 is down
Hello Histology friends, We are a research lab with a 5 year old Leica ST5020 autostainer that looks like new. We run less than 5000 slides a year on it. We noticed that (of course just after the warranty ended) that one of the water rinse stations stayed full of water all the time, even when the instrument was off. It may have been like this from the beginning and we just did not notice it. Leica quoted a $6000 repair but said we could run it as is. So, we skipped the repair and went on using it. Now the instrument is completely down and the repair is a new "slave controller board" for $17,600, stepper motor for arm, etc. with total quote for over $23,800. Has anyone else had major issues with this instrument over time. We are trying to decide whether to fix it or go for a new stainer. I'm kinda wondering if we got a lemon. Thank you for your time, Cathy Cathy M. Mathis Lab Technician IV Dept. of Pathology \ Comparative Medicine Wake Forest School of Medicine p 336.716.1538 \ f 336.716.1515 cmmat...@wakehealth.edu<mailto:cmmat...@wakehealth.edu> www.wakehealth.edu<http://www.wakehealth.edu> ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TUNEL on Leica Bond
Hello Histo-friends, Is anyone doing a TUNEL stain on any of the Leica Bond instruments? I will be attempting to set this up and thought I would reach out first. Thank you, Cathy Cathy M. Mathis Laboratory Technician IV -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Friday, October 19, 2018 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 179, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg=HlJCIs0uAvNmmGWdd-pEFIDvdEBtEL2uYCMe-3yHqVk=19Ow0ZV3sn97Uww-nCnsPHdxQtOFWY-zJR4M1RFnuE8= or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. toluene substitute (Michelle Jamison) 2. RUO antibody (Erin McCarthy) 3. Re: Restaining old Heamatology smears (Eddie Martin) 4. Re: BMP-2 protocol for bone (Eddie Martin) -- Message: 1 Date: Thu, 18 Oct 2018 14:48:50 -0600 From: Michelle Jamison To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] toluene substitute Message-ID: Content-Type: text/plain; charset=utf-8; format=flowed Hi All, I am from industry. We are interested in developing an instrument that stains then prepares microscope slides to be coverslipped. If you do coverslip, what substitute do you use instead of toluene to go to mounting media? Thank you! Michelle -- All the best to you, Michelle Jamison /Bio Research Associate / /ELITechGroup Inc. / ???370 W. 1700 S.?? ???Logan Utah ??? USA m.jami...@elitechgroup.com <mailto:m.jami...@elitechgroup.com> ? _https://urldefense.proofpoint.com/v2/url?u=http-3A__www.elitechgroup.com=DwICAg=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg=HlJCIs0uAvNmmGWdd-pEFIDvdEBtEL2uYCMe-3yHqVk=zEZUJnDPDmAli_gIL21jiQLLLktKgspCpja5mtUKhpg= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.elitechgroup.com_=DwICAg=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg=HlJCIs0uAvNmmGWdd-pEFIDvdEBtEL2uYCMe-3yHqVk=cner0ipmokPcpr_NQBGSwwWLZ80at5dbC8SJjotXLMI=> _ Tel : +1.435.752.6011 Ext: 1474 Logo -- Message: 2 Date: Thu, 18 Oct 2018 16:30:14 -0500 From: Erin McCarthy To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RUO antibody Message-ID: Content-Type: text/plain; charset="UTF-8" Hi All, I am looking to see if anyone has had any experience with the antibody AXL. We are trying to work it up on our Roche Discovery Ultra and it does not seem like it stains both nuclear and cytoplasic cell features. We only seem to see cytoplasmic staining, and in general most tumors are showing negative results. I currently am using the Ultraview kit for optimization, our pathologist likes CC2 for 44 min, Ab. Inc for 40 min using a 1:100 dilution. Anyone have any suggestions that might help? If you want more detailed info I can provide as well! -- Erin McCarthy, HT (ASCP) Pathology Lab Supervisor Tempus Labs 600 W. Chicago Ave. Chicago IL 60654 Ph:(312) 638-6344 Ext.3835 -- This email and any attachments may contain privileged and confidential information and/or protected health information (PHI) that is protected by federal and state privacy laws.? It is intended solely for the use of Tempus Labs and the recipient(s) named above.? Nothing contained in this communication and any attachments thereto is intended to waive any privileges or rights of confidentiality.? If you are not the recipient, or the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any review, dissemination, distribution, printing or copying of this email message and/or any attachments is strictly prohibited.?* If you have received this transmission in error, please notify us immediately at?**(855)-442-8305**? and permanently delete this email and any attachments*. -- Message: 3 Date: Thu, 18 Oct 2018 18:20:53 -0400 From: Eddie Martin To: "Reilly, Laurie" Cc: "Histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] Restaining old Heamatology smears Message-ID: <0e7f124e-9821-4a32-a438-63f45ad45...@gmail.com> Content-Type: text/plain; charset=utf-8 Hi Laurie, Blood is considered a tissue so it
Re: [Histonet] Leica Bond Max artifact
Hello Dr. Rosen and Histonetters, We have used a Leica Bond RX for over 2 years now, cleaning and caring for it just as you do. My work is mostly animal which is why we mainly choose the red chromogen alkaline phosphatase detection kit. We see the same artifact sporadically. Sometimes we will see it for several days and then maybe a week goes by and we don't see any of it. It may only be a small amount and I have seen it basically all over the slide. I know it is not related to our tissues or slides. Our Leica rep came out and increased washes but it really doesn't help. If anyone has a solution to the problem, I would also be interested. Best to all, Cathy Cathy M. Mathis Laboratory Technician IV Department of Pathology Section on Comparative Medicine - Clarkson Campus Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.1538 \ f 336.716.1515 cmmat...@wakehealth.eduwakehealth.edu -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Wednesday, October 10, 2018 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 179, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet=DwICAg=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw=65VTIbTt5Cez3DHVF4vIBCoqH0MsYay3VSIywTmpZfw= or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: Leica Bond Max artifact (Linda Margraf) 2. Re: FW: Leica Bond Max artifact (Rene J Buesa) 3. H for H. pylori? (P Sicurello) 4. Re: H for H. pylori? (Rene J Buesa) 5. Re: H for H. pylori? (Tony Henwood (SCHN)) 6. Job Opening in Massachusetts: IHC Lab Supervisor (Erin Sarricks) 7. Low Oxygen Sensor/Alarms (Cooper, Brian) -- Message: 1 Date: Tue, 9 Oct 2018 17:06:27 + From: Linda Margraf To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] FW: Leica Bond Max artifact Message-ID: Content-Type: text/plain; charset="utf-8" Here is a message I am posting for Dr. Rosen: Hello, I am a longtime admirer of this list, and I am sure someone on here can help us out We have an artifact on our immunohistochemistry (IHC) slides. I will attempt to attach pictures using the upload tool. In case that doesn?t work, I am attaching a google drive link to some jpgs. The artifact is usually golden brown to red/pink in color, is clustered, appears both on top of the tissue and around it. If we do an aggressive rundown, it appears less red/brown and bluer. We are using a Leica Bond MAX IHC system which is about a year old. We usually do two runs a day and 95% of the time we only use a red detection kit. We only see this on our red detection kit on our Leica bond. The brown is unaffected. The corresponding H slides are fine. We started having this artifact after a month or two of production. 85% of the time we have some sort of artifact. Sometimes its perfect, sometimes it makes the tissue unreadable. We haven?t figured out any precipitating factors. We have tried a number of things, but we still can?t get control of this. Here is a brief overview of our protocols: - Processor: Leica ASP300s tissue processor. -Parrafin = Mercedes HWWX (dual purpose embedding and infiltration parrafin) melting point 57c ? we tried switching, and it didn?t help. - We only use distilled water in our baths - Mercedes Starfrost charged slides - Oven for 30 min at 62c - We always use a cold red detection kit -Sometimes our runs are delayed but not more than 4 hours - Rundown is done in our stainer (standardized), and slides are coverslipped with tape. - The bulk fluid containers in the bond are cleaned weekly and refrigerated between runs. - The probe is cleaned every 300 slides. - Mixing wells are cleaned out every two weeks and replaced often. -Covertiles are well tended to. We have tried to correct this problem, but we can't seem to fix it. Any ideas? https://urldefense.proofpoint.com/v2/url?u=https-3A__drive.google.com_open-3Fid-3D1wUttTweP7SYLRM5pkGOBr0MBzCMrtJSA=DwICAg=yzGiX0CSJAqkDTmENO9LmP6KfPQitNABR9M66gsTb5w=ubybF7_hjNk08A-Q08Mf7WNmad5a9znkJq3XozlNVlg=UD2XLalsIAuK_lUZgavLUGnMMJaE8-g4Q28xJLvJLAw=kyqBaPGz-SvAoNDooiBtkSemt9dk4eOKo8l1XYvtmIs=<https://urldefense.proofpoint.com/v2/url?u=https-3A__drive.google.com_open-3Fid-3D1wUttTweP7SYLRM5pkGOBr0MBzCMrtJSA
[Histonet] Questions concerning Leica Bond RX
Hello Histo-netters! I have a Bond RX and before I try to recreate the wheel, I thought I would ask some experts. Has anyone used a goat primary on this instrument and if so how? Also, has anyone had success with TUNEL on this instrument and if so, could you share your knowledge? Thank you all for your time, Cathy Cathy M. Mathis Laboratory Technician IV Department of Pathology - Section on Comparative Medicine Clarkson Campus Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.1538 cmmat...@wakehealth.edu -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Wednesday, September 28, 2016 12:33 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 154, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Water for H Stainers? (Hannen, Valerie) 2. Position announcement (Leslie, Mary) 3. Sandersons Rapid Bone stain chemistry and history (Gayle Callis) 4. Re: water for stainers (Terri Braud) 5. H.pylori question (Gareth Davis) 6. buffered zn-formalin recipe needed (Preiszner, Johanna) 7. Re: GMS (Caroline Miller) 8. Re: H.pylori question (Kienitz, Kari) 9. ATRX Antibody (Marcum, Pamela A) 10. Re: Water for H Stainers? (cfitz) 11. Re: Water for H Stainers? (WILLIAM DESALVO) 12. Re: Histonet Digest, Vol 154, Issue 22 (Nasir Abbas) 13. HT grossing competencies (Farah Nawaz) 14. looking for a cryostat service tech. in the Houston area. (Hebert, Pamela) 15. EXAKT band saw blade (Garcia, Lori, M.Sc.) -- Message: 1 Date: Tue, 27 Sep 2016 13:10:05 -0400 From: "Hannen, Valerie" <valerie.han...@parrishmed.com> To: 'P Sicurello' <pat...@gmail.com> Cc: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] Water for H Stainers? Message-ID: <450B7A81EDA0C54E97C53D60F00776C323E455E63B@isexstore03> Content-Type: text/plain; charset="us-ascii" Ours is hooked up to DI water. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com www.parrishmed.com -Original Message- From: P Sicurello via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, September 27, 2016 12:28 PM To: HistoNet Subject: [Histonet] Water for H Stainers? Good Morning Everyone, What type of water do you use with your automated H stainers? House or deionized/distilled? We cannot buy one of the waterless, fancy schmancy H stainers at this time. L Thank you in advance. Sincerely, Paula Paula Sicurello, HTL (ASCP)CM Histotechnology Specialist UC San Diego Health 200 Arbor Drive San Diego, CA 92103 (P): 619-543-2872 *Confidentiality Notice*: The information transmitted in this e-mail is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this e-mail in error, please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet == "This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you" == -- Message: 2 Date: Tue, 27 Sep 2016 17:13:54 + From: "Leslie, Mary" <mary.les...@tricore.org> To: "histonet@lists.utsouthwestern.edu&qu
Re: [Histonet] CD34 for primate tissue
This is a great website for NHP info. Thank you all for all your wonderful help!! Hope everyone has a great 4th of July weekend Cathy -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Friday, July 01, 2016 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 152, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: How to get your stains more vibrant when cutting at 2?m? (Angela Lamberth) 2. Re: CD34 for primate tissue (Croix, Denise) 3. Just some before the long weekend Friday Fun-lab related blog (Lester Raff MD) 4. Certification (Adesupo, Adesuyi (Banjo)) -- Message: 1 Date: Thu, 30 Jun 2016 10:41:38 -0700 From: Angela Lamberth <alambe...@lji.org> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] How to get your stains more vibrant when cutting at 2?m? Message-ID: <canunjwni6rswkgqvp5u7-wn3r29gk4mov_gr-ykwa41vtjt...@mail.gmail.com> Content-Type: text/plain; charset=UTF-8 Thank you all very much for your suggestions. I'm going to play around with a progressive H when I return from vacation next month. I do have safranin on hand but will need to order some phloxine to experiment with. I will probably need to order some additional supplies to make the hematoxylin. I'm not sure yet if I'll start with Mayer's or Gill's or maybe even Erlich's. I should note that cutting at 2 isn't required, but it is desired once they saw that I can. And since I can, I aim to please! :-) In addition, I'm looking forward to trying the oven dry method before coverslipping. A rapid dehydration isn't really possible since I'm working with a xylene substitute (Pro-Par) and have been battling eosin carryover but that is a whole different animal for another thread. Thanks again! I'll report back in a month or 2. On Wed, Jun 29, 2016 at 10:27 AM, Rene J Buesa <rjbu...@yahoo.com> wrote: > > Angela: > "Pale" results are the trade-off for great quality very thin "2 ?m" > sections but you can always improve intensity somewhat . > 1- your "regressive" stain, if it is "modern Harris" has the inherent > problem of lacking mercury chloride and it is little you can do about. > Perhaps if you use "progressive Mayer" you will get better results. > You will not have to differentiate (with the intrinsic "danger" of > leaving the section too pale) and if used fresh Mayer's can be a good > approach. > 2- as to the counterstain perhaps you should add safranine to the > eosin (20% safranine + 80% eosin) and will get a darker red. > 3- try to dehydrate as quickly as possible or even better, wash the > sections in distilled water and place them in an oven at 60?C for 10 > minutes and coverslip as usual. You will eliminate any "color wash" > due to the alcohols. > If you've not enough "trust" on dry/oven dehydration, try with some > sections as a test. You will like the method. > Ren? > > > On Tuesday, June 28, 2016 5:53 PM, Angela Lamberth via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > When I cut at 2?m my H and special stains look pale. How can I get > my stains to pop or am I stuck with pale looking stains when > sectioning that thin? > > I run manual specials and a manual regressive H For H I've tried > increasing my time in hematoxylin (beyond the manufacturer > recommendation), diluting my acid alcohol differentiation, and > increased time in eosin but the slides still lack the vibrancy that many of > the postdocs desire. > > I use Shandon instant hematoxylin and alcoholic eosin by Thermo. > Everything else I prepare in house from scratch. Any recommendations? > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- Angela Lamberth Histology Technician II Histology Core Lab La Jolla Institute for Allergy & Immunology 9420 Athena Circle La Jolla, CA 92037 -- Message: 2 Date: Thu, 30 Jun 2016 16:57:53 -0500
Re: [Histonet] CD34 for primate tissue
Rhesus is a species of old world monkey. There are a couple of other companies that have this same clone and on their datasheets they say it cross reacts with rhesus monkey. The CD34s (2) that I have do not state on their data sheets that they cross react with anything except human and yet are the same clone as the companies that say it does. I guess I will just break down and purchase a different clone that states it works in rhesus monkey. Cathy -Original Message- From: Eddie Martin [mailto:edmarti...@gmail.com] Sent: Wednesday, June 29, 2016 8:22 PM To: Cathy M. Mathis/Comparative Medicine Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CD34 for primate tissue What species is the kidney you are trying to stain with IHC. Because you mentioned that you used normal human kidney and it worked , but Rhesus kidney didn't stain. I'm wondering if the Rhesus kidney is human or another animal species? The Novocastra CD34 (QBEND10) is intended for human tissue. Best, Eddie Martin, HTL,QIHC Sent from my iPhone > On Jun 29, 2016, at 4:43 PM, Cathy M. Mathis/Comparative Medicine > <cmmat...@wakehealth.edu> wrote: > > 2 more bits of information about my dilemma; I did try staining > without any retrieval - no signal I am using rhesus kidney as a > control (getting no signal), but I also ran some human kidney and got > beautiful staining with a 20 minute HIER using a pH 8 EDTA buffer. > So I know the antibodies and my protocols work, just not on my rhesus. Does > anyone know of a CD34 that will work in this species? > More suggestions please? Thank you all for your time. > Cathy > > -Original Message- > From: Eddie Martin [mailto:edmarti...@gmail.com] > Sent: Wednesday, June 29, 2016 5:34 PM > To: Cathy M. Mathis/Comparative Medicine > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CD34 for primate tissue > > Hi Cathy, > > EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And > all that is necessary. Please contact me if you need additional help. > > Best, > Eddie Martin, HTL, QIHC > edmarti...@gmail.com > > Sent from my iPhone > >> On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine >> <cmmat...@wakehealth.edu> wrote: >> >> Good morning my fellow Histo-netters, Does anyone have experience >> with any CD34 antibody that would work in FFPE rhesus tissues? A few >> companies have one with clone QEBend 10 and say that it works but I have had >> no luck. I am using the Bond RX polymer system and I've tried all the >> epitope retrievals from Leica. Now I am try epitope retrieval offline; >> pronase, trypsin, pressure cooker with high and low pH solutions. Still no >> signal. Any help would be greatly appreciated. >> Cathy > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] CD34 for primate tissue
2 more bits of information about my dilemma; I did try staining without any retrieval - no signal I am using rhesus kidney as a control (getting no signal), but I also ran some human kidney and got beautiful staining with a 20 minute HIER using a pH 8 EDTA buffer. So I know the antibodies and my protocols work, just not on my rhesus. Does anyone know of a CD34 that will work in this species? More suggestions please? Thank you all for your time. Cathy -Original Message- From: Eddie Martin [mailto:edmarti...@gmail.com] Sent: Wednesday, June 29, 2016 5:34 PM To: Cathy M. Mathis/Comparative Medicine Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CD34 for primate tissue Hi Cathy, EDTA (Retrieval #2) for 20 minutes on the Bond-Rx should be sufficient. And all that is necessary. Please contact me if you need additional help. Best, Eddie Martin, HTL, QIHC edmarti...@gmail.com Sent from my iPhone > On Jun 29, 2016, at 8:16 AM, Cathy M. Mathis/Comparative Medicine > <cmmat...@wakehealth.edu> wrote: > > Good morning my fellow Histo-netters, > Does anyone have experience with any CD34 antibody that would work in FFPE > rhesus tissues? A few companies have one with clone QEBend 10 and say that > it works but I have had no luck. I am using the Bond RX polymer system and > I've tried all the epitope retrievals from Leica. Now I am try epitope > retrieval offline; pronase, trypsin, pressure cooker with high and low pH > solutions. Still no signal. Any help would be greatly appreciated. > Cathy > > > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD34 for primate tissue
Good morning my fellow Histo-netters, Does anyone have experience with any CD34 antibody that would work in FFPE rhesus tissues? A few companies have one with clone QEBend 10 and say that it works but I have had no luck. I am using the Bond RX polymer system and I've tried all the epitope retrievals from Leica. Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions. Still no signal. Any help would be greatly appreciated. Cathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] working Dako Autostainer, OR/WA area make offer
We have upgraded to a Dako Link 48 machine and have a working Dako Autostainer system to get rid of. The machine was purchased new in 2000 and has been well maintained. The working computer and printer are included, as well as jugs, manual, and software backups. Our biomed department doesn't want to crate it up, but if there is someone in the Portland Oregon area who wants to come, package it, and take it off of our hands feel free to make us an offer. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 133, Issue 13
Are you filtering your trichrome stain just before using it? Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, December 11, 2014 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 133, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Gomori Trichrome (Joanna) 2. Lab power failures (Martin, Erin) 3. IHC CONTROL LOG (sidonie.a...@hcahealthcare.com) 4. RE: Gomori Trichrome (Morken, Timothy) -- Message: 1 Date: Wed, 10 Dec 2014 21:18:41 -0800 From: Joanna jobalu...@gmail.com Subject: [Histonet] Gomori Trichrome To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 66d86405-5915-4d8b-93cc-d8125b122...@gmail.com Content-Type: text/plain; charset=us-ascii Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada -- Message: 2 Date: Thu, 11 Dec 2014 10:37:37 + From: Martin, Erin erin.mar...@ucsf.edu Subject: [Histonet] Lab power failures To: histonet histonet@lists.utsouthwestern.edu Message-ID: 24b7b291cc88d04ab663958e77a1f59d1d0...@ex09.net.ucsf.edu Content-Type: text/plain; charset=iso-8859-1 Good morning all! Would anyone mind sharing their plans for power failure? We have backup generator power on red plugs but my pathologists want a plan for what we would do if the power went out AND the backup generator failed. Thanks for your help! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. -- Message: 3 Date: Thu, 11 Dec 2014 16:15:00 + From: sidonie.a...@hcahealthcare.com Subject: [Histonet] IHC CONTROL LOG To: histonet@lists.utsouthwestern.edu Message-ID: 8e35b71d4c94c14ba935dac7b93b4670a...@xrdcwpmsghcmd2c.hca.corpad.net Content-Type: text/plain; charset=us-ascii BEEN DOING IHC FOR TWO YEARS. WOULD LIKE TO KNOW HOW OTHERS ARE LOGGING THEIR CONTROL CASES. THANKS, SIDONIE -- Message: 4 Date: Thu, 11 Dec 2014 16:18:39 + From: Morken, Timothy timothy.mor...@ucsf.edu Subject: RE: [Histonet] Gomori Trichrome To: 'Joanna' jobalu...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 761e2b5697f795489c8710bcc72141ff367bf...@ex07.net.ucsf.edu Content-Type: text/plain; charset=us-ascii Joanna, If it looks like it is under the section, then I would suspect trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanna Sent: Wednesday, December 10, 2014 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome Hi, I was hoping you guys can help us with our problem with modified
[Histonet] RE: Gomori Trichrome
Have you tried filtering the stain just before using it? Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, December 11, 2014 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 133, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Gomori Trichrome (Joanna) 2. Lab power failures (Martin, Erin) 3. IHC CONTROL LOG (sidonie.a...@hcahealthcare.com) 4. RE: Gomori Trichrome (Morken, Timothy) -- Message: 1 Date: Wed, 10 Dec 2014 21:18:41 -0800 From: Joanna jobalu...@gmail.com Subject: [Histonet] Gomori Trichrome To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 66d86405-5915-4d8b-93cc-d8125b122...@gmail.com Content-Type: text/plain; charset=us-ascii Hi, I was hoping you guys can help us with our problem with modified gomori trichrome stain for frozen muscle biopsy. We've been seeing a lot of red blotches on our slides. We've tried 3 different manufactures of chromatrope 2 R dyes, including changing our recipe. So far no success. Hopefully you can give us some suggestions. Thanks. Neuropathology lab Vancouver, Canada -- Message: 2 Date: Thu, 11 Dec 2014 10:37:37 + From: Martin, Erin erin.mar...@ucsf.edu Subject: [Histonet] Lab power failures To: histonet histonet@lists.utsouthwestern.edu Message-ID: 24b7b291cc88d04ab663958e77a1f59d1d0...@ex09.net.ucsf.edu Content-Type: text/plain; charset=iso-8859-1 Good morning all! Would anyone mind sharing their plans for power failure? We have backup generator power on red plugs but my pathologists want a plan for what we would do if the power went out AND the backup generator failed. Thanks for your help! Erin Erin Martin, Histology Supervisor UCSF Dermatopathology Service 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or priviledged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. -- Message: 3 Date: Thu, 11 Dec 2014 16:15:00 + From: sidonie.a...@hcahealthcare.com Subject: [Histonet] IHC CONTROL LOG To: histonet@lists.utsouthwestern.edu Message-ID: 8e35b71d4c94c14ba935dac7b93b4670a...@xrdcwpmsghcmd2c.hca.corpad.net Content-Type: text/plain; charset=us-ascii BEEN DOING IHC FOR TWO YEARS. WOULD LIKE TO KNOW HOW OTHERS ARE LOGGING THEIR CONTROL CASES. THANKS, SIDONIE -- Message: 4 Date: Thu, 11 Dec 2014 16:18:39 + From: Morken, Timothy timothy.mor...@ucsf.edu Subject: RE: [Histonet] Gomori Trichrome To: 'Joanna' jobalu...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 761e2b5697f795489c8710bcc72141ff367bf...@ex07.net.ucsf.edu Content-Type: text/plain; charset=us-ascii Joanna, If it looks like it is under the section, then I would suspect trapped reagent in air bubbles, folds, etc. If it is on top of the section then maybe more rinsing is needed. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanna Sent: Wednesday, December 10, 2014 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Gomori Trichrome Hi, I was hoping you guys can help us with our problem with modified gomori
[Histonet] Part time histotech needed in OR
We just posted a part time HT position at Tuality Community Hospital in Hillsboro, Oregon. Go to Tuality.org under careers to apply. Tuality has a great pathology lab that is only open M-F. Position is for 24 hours per week with benefits. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Setting up ASR antibody to meet CAP requirements
For the first time my pathologist would like to bring an ASR antibody in house. It is made by Biocare, and our validation slides look good compared to the other clone we want to get rid of. However, I see in question 12425 of my checklist that I have to establish or verify the performance characteristics... in accordance with the Method Performance Specifications section of the All Common Checklist. Is there anyone who would be willing to share their procedures on verifying and validating ASR's for IHC purposes? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] lower glands in colon not staining with IHC
On our AE1/AE3 and CK 20 slides we are noticing a deminished staining in the lower glands of the colon sections. My first thought was inadequate fixation, however the BerEP4 stained evenly. This has been happening on different tissues, including small biopsies. Does anyone have any ideas as to something else that could be causing a lack of staining on the internal colon portions? We use Dako reagents and polymer kit and the skin and other tissues in the sausage block stain beautifully. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] bone marrow aspirations
We are using a similar method at our site. The aspirate is placed into EDTA and then into formalin to fix. We pour the aspirate through a biopsy bag, open the bag and scrape the spicules together, transfer them to another biopsy bag and process as usual. I have received several compliments on the quality of the slides. Cathy Kelowna, B.C. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shea's Sent: Saturday, June 1, 2013 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow aspirations I am also interested in how others are processing bone marrow aspirates. Currently, we let the aspirate clot on a watch glass for about 20 minutes, gentle slide it onto filter paper, roll it around to get rid of the excess fluid, place the clot between sponges, and process as usual. However, I am interested in knowing if any one out there is using a technique that we use to use at another hospital 33 years ago. If I remember correctly, the pathologist or oncologist would collect the aspirate using a heparin syringe. Some how, the aspirate smears for Hematology were made on 22x22 cover slips instead of slides. Then the EDTA tube was then given to Histology. I can't remember if we added formalin or B5 fixative to it at that time, but it was filtered into an obex tea bag type filter paper (The blood never clotted and ran through the filter paper, leaving only bone marrow spicules in the paper). We would fold the paper, place it in the cassette, and process as usual. The pathologist preferred this method because he wouldn't have to look at levels of clot to find spicules. Is there anyone out there using this method? Jan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] AutoTech Embedder( Sakura)
I am also interested in information on the Sakura AutoTech Embedder. My manager has put on the capital equipment list along with the Sakura Express processor. Thanks, Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiranjit Grewal Sent: Thursday, April 18, 2013 10:13 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: [Histonet] AutoTech Embedder( Sakura) Hello, I really need some feedback on this before we invest in this technology. Thanks. Kiran Sent from my iPhone Begin forwarded message: From: Kiranjit Grewal kira...@sbcglobal.net Date: April 17, 2013, 2:25:36 PM PDT To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AutoTech Embedder( Sakura) Hello Dear Colleagues, We are considering Sakura's AutoTech Embedder for embedding some of the tissues especially Large tissue. Any input or feedback is much appericiated. Anyone currently using it and would like to share their experience is great. Thank you, -Kiran ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PIN4 staining
We use Bond slides and have noticed that occasionally they seem to have an extra thick coating on them that will pick up DAB and turn slides brown. If the glass itself is turning brown this might be your issue. Have you tried different slide lots or brands? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sakura glas coverslipper and mounting media types
For those with the Sakura Glas coverslippers, what type of mounting media are you using in these? Sakura recommends only their brand of course, but I want something that dries faster. We do not have excess mounting media on the outside of our slides, but after being in the oven for three days we had several slides sticking. It looked like the mounting media slipped down the slide to pool at the bottom and stick. I am thinking their media is too viscous. If I turn the amount down on the machine any further we end up with not enough media on the slides and get large air pockets after just a few days of storage. BTW the oven is at 40 degrees and isn't boiling hot to dry the slides. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IVD P63
I was just told by my Biocare rep that today was the last day they are selling P63 antibody. I would like to purchase IVD P63 from another vendor, who else sells it? I have having a few problems finding good info just using Google. What is your lab using for diagnostic purposes? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IVD P63
Yes, it is true. I just talked with Biogenex Laboratories and they will be carrying both the 4A4 and 4B1E12 clones in the foreseeable future. Other places I have talked to are depleting their stock and not carrying P63 anymore. Ventana wants $800+ dollars for 50 tests worth and do not carry concentrates. What a racket! Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Patient requests for tissues
I am wondering how other hospital connected histology labs handle requests from patients to get their tissue back. I am especially interested in the placentas. Our pathologists and managers are struggling to come up with a policy that is sensitive to various religions and cultures but keeps everyone safe. Do you allow patients to get back their specimens after the case is signed out or not??? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Interview Questions
The last two times we interviewed a person we gave them a written skills test before they sat down for the face to face interview. It had simple questions on it, like how to mix a percent solution, clean a formalin spill, which chemical was more hazardous than another, how to handle certain types of tissues, how to work computer applications, etc. The test has 15 questions and has a section that includes them writing on a cassette and following written instructions on how to prep a non-gyn cytology. This gave us a lot of skills information about the person and helped us weed out who was qualified. You could tell quite a bit about their knowledge just from the written portion and people seemed less nervous when there wasn't a person hovering over them. Our hospital sees this skills test as the equivalent of a typing test for transcriptionists. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Population Served compentency requirements
I was wondering if anyone else was having to come up with a histology department competency with a focus on population served. From what I understand it is a Joint Commission requirement for our hospital annual competencies. We are having a hard time coming up with something because we do not work with the public, just their specimens. Any ideas??? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Histology lab assistant in CA
Hello everyone, I have a wonderful histo lab assistant who is moving from Oregon to the Lathrop, CA area. We absolutely hate to see her go, but must. If there is anyone with an assistant position open in that area would you please let me know so I can pass the info on to her. She would be a real asset to your team. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] repetitive wrist injury
Has anyone experienced problems with the histotechnologists or Pathologist's Assistants developing wrist injuries from opening the small specimen containers all day on the grossing bench? If so, how did you address this issue? We have had occupational health assess the process; there haven't been any suggestions yet. Cathy Kelowna, B.C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Part time postition for histology lab assistant
We have a part time with benefits histology lab assistant positio= n open in Hillsboro, OR. Go to [1]www.Tuality.org for more information if interested. W= e are looking for someone to gross with the pathologists and handle the acc= essioning and closing of our department. The hours are M-F 12-5 pm. Cathy Crumpton HT(ASCP), Histology Lead Tu= ality Community Hospital Hillsboro, OR 97123 (503)681-1292 = /FONT References 1. 3Dhttp://www.Tuality.org/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] eosin
We add 1.0 mls of 10% aqueous Eosin to the first 100% Ethanol; we will then have Eosin in all of the Ethanol containers after they have been rotated. This removed the time consuming task of dotting each tissue at grossing and allows for easy visualization at embedding and sectioning. I have not had a complaint about the eosin interfering with FISH from our referral lab. Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital _ From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot...@imagesbyhopper.com Sent: Friday, October 22, 2010 8:18 AM To: Tench, Bill Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] eosin It sounds like I am in the minority in pitting the eosin in the *first* 100% alcohol! I agree with Bill, it really makes a HUGE difference in small, minute tissues. We did run into one issue though, we were using orange biopsy cassettes and the red-orange tissue was difficult to spot in the orange cassettes! We have since switched to an aqua color and no more hiding specimens! ;o) Michelle On Oct 22, 2010, at 10:51 AM, Tench, Bill bill.te...@pph.org wrote: I think the use in a processor becomes a local culture. I think everyone in my community does it (someone spread the word), so now we all do. I believe that we are all putting it in the last alcohol. It really makes facing a block on minute pieces a whole lot easier. We found marking the tissue directly tedious, and it didn't persist as well in the processing. Because there is often so little visible material in our FNA cell blocks, we mark the histogel pellet with Davidson ink, and when the tech has just faced off the ink, he/she knows its time to start collecting sections. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations - Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1136 / Virus Database: 422/3212 - Release Date: 10/22/10 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Need opinions on HE stainers please!
We are using the Sakura Prisma with attached coverslipper; it is a workhorse and allows for the use of what ever reagents/stains that you prefer. We are running our HE and H Pylori stains simultaneously. We experienced a small problem with the leveling of the instrument at the initial set up but once that was corrected it has been working well since then. The only draw back that I can see with the attached coverslipper is that the slides take longer to dry than with the older model. The slides are kept horizontal rather than vertical so the xylene doesn't run off. Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital _ From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kendall Neely Sent: Friday, October 22, 2010 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need opinions on HE stainers please! Our laboratory will be purchasing new HE stain equipment in the near future. We would like to hear the pros and cons out there for the Thermo-Shandon Gemini, Sakura Prisma, Leica Autostainer XL and the Ventana Symphony. Thanks in advance for your input. Kendall A. Neely Histology Technical Specialist Shands Rocky Point Laboratories (352) 265-0111, x72113 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ No virus found in this message. Checked by AVG - www.avg.com Version: 10.0.1136 / Virus Database: 422/3212 - Release Date: 10/22/10 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CAP question ANP.22970
How is everyone handling this new question about Annual Result Co mparison for ER and PR? The question states ...the laboratory at lea= st annually compares its patient results with published benchmarks, and eva= luates interobserver variability among the pathologists in the laboratory..= . Do you have a separate computer program or do this by hand? = DIV Cathy Crumpton HT(ASCP), Histology Lead Tuality= Community Hospital Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 83, Issue 19
We are not open on weekends and worked out a deal with our core l= ab that is open 24/7. When we have a breast on the processer we willrun a Sat. program that ends at 21:00. They added a task on their dai= ly checklist to come up and take the tissues off at that time. They j= ust sit them on the counter and they cool - just like they were being embed= ded. When we come in on Monday we just sit them in the embedding cent= er for 15 minutes and they warm up enough to start embedding. No harm= to tissues and no one has to come in. Cathy= Crumpton HT(ASCP), Histology Lead Tuality Community Hospital Hillsbo= ro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] problems with staining IHC
We and several other sites (in British Columbia and Alberta) that I have contacted have been experiencing IHC staining problems. Ours started in August while others have been seeing this since May. We have had conference calls with Ventana about this and they have said that it is a problem with the SuperFrost Plus slides; the slides seem to be hydrophobic. The staining that we are seeing is negative controls/positive patient, positive control/negative patient as well as variable counterstain. We have control tissue on every slide. From what we have learned from Fisher and Ventana they are working together with Erie Scientific (manufacturer of the Plus slides) to find out what is causing the problem. The slides appear to be vary from box to box and within each box. Some lot numbers are worse than others; our most recent lot number was 062510-9. I would suggest that you contact your sale person as well as the platform vendor (if your staining is automated) so they are aware of how large the problem may be. We have not found another slide that is any better than the Plus. Ventana is working on an official communication for their clients. We are very frustrated with the lack of progress in solving the problem. As anyone noticed that the Plus slides don't seem to have the same adhesive properties that they used to? Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital Kelowna, B.C. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Thursday, October 07, 2010 7:32 AM To: histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Skills testing for a histo lab assistant
We are going to be posting a histology lab assistant position soo= n for the first time. I would like to give them a skills assessment t est to see how well they can handle a computer, numbers, problem solving, a= nd what their handwriting looks like. Does anyone out there do this f= or their new hires? What and how do you do to test? nb= sp; Cathy Crumpton HT(ASCP), Histology Lead Tuality Community = Hospital Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Technical Assistant or plain Lab Assistant?
I need help from my fellow histotechs. We are creating a po= sition for a histology lab assistant. We need someone to assist the p athologist when grossing (not grossing themselves), clean machines, file, d= ata entry and accessioning, etc. We are trying to decide if this posi= tion should be a Technical Assistant job code or a plain Lab Assistant code= . I wanted to know if you have any such assistants in your area and w= hat their category was. If it is a technical assistant, what exactly = makes the position worth the higher pay grade? This is difficult beca= use there in not a certification for a histology lab assistant and they do = not run analyzers or testing machinery. Cath= y Crumpton HT(ASCP), Histology Lead Tuality Community Hospital Hillsb= oro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Attention new equipment distributors
Can you give me a ball park figure for a new paraffin embedding center? I have a friend setting up a new lab and are working on their budget. They need a cost of a unit equivalent to the Shandon Histocentre that has the electric forceps. About 7 years ago I paid about $7000 for a unit such as this but of course time and costs have changed things. Please respond off line to camay...@gmail.com Thanks in advance, -- Cathy Mayton ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] xylene in an unventilated area
Hello all. Cytology was told that the xylene substitute the= y were using for their Surepath paps was causing staining/artifact issues. nbsp; They want to switch to using xylene in their area, but it is unventil= ated. Because of various reasons, their process can not be moved.nbs= p; They are asking me for advice about what to do for fume control. I= was wondering if a good downdraft unit would be enough to handle the xylen= e fumes. They will have maybe 400 mL of xylene uncovered while covers= lipping their paps. Does anyone else have a similar set up? The= y share their area with micro and core lab and others are complaining about= fumes even though they have borrowed a mini-hood from us. Badge resu= lts are still pending. Cathy Crumpton HT(ASC= P), Histology Lead Tuality Community Hospital Hillsboro, OR 97123 = (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] attention equipment resellers/refurbishers
I have a 6 foot external exhaust fume hood with blower that I need to find a home for. It has a fairly large sink in the left side of the countertop and 1/2 of the base cabinet is for flammable storage. If interested please contact me personally at camay...@gmail.com. I can send pictures to interested parties. -- Cathy Mayton ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Biocare P63 with Dako Flex detection
Hi all, I have ran into a troubleshooting dilemma and was wonderi= ng if anyone else could help. Is there anyone out there that uses the Biocare Medical P63 concentrated antibody with the Dako TRS and Flex detec= tion kit? I am curious what dilution you use and if you have to do an= ything special for the P63 (extra long retrieval, DAB enhancer, etc). = I am having problems getting a strong signal for the basal cells in prosta= te. I am running it at 1:50 with 40 minutes in pH 9 retrieval (which = seems too long to me but does help). A dilution with the same protoco= l at 1:100 is way too light. Thanks, = /DIV Cathy Crumpton HT(ASCP), Histology Lead Tuality Community Hosp= ital Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Can anyone use some reagent?
Hello histonet. I have some Dako reagets that we do not nee= d anymore. We have switched from the LSAB2 system to the Flex polymer and have 2 unopened containers of dilutent (S0809) and one unopened contai= ner of TRS (S1699) that we can not use. If anyone wants to pay for sh= ipping you can have them. I would rather see them used than thro= wn away. I also have a barely used container of Halt waterbath ad= ditive that someone can have if they want it. Our lab did not like th= e slight chemical odor given off by the additive. Please email me off= list. Cathy Crumpton HT(ASCP), Histology LeadTuality Community Hospital Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Ventana Ultra
We are in the process of upgrading our immuno stainers to the Ventana Ultra. I am interested in how people validate a new instrument and how the Ultra was integrated or changed lab workflow. Cathy Kelowna, B.C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] liquidating lab equipment
I have a few pieces of lab equipment left over from when I closed and liquidated the lab. I have the following: 1) Shandon paraffin embeddding center with heated forceps 2) VIP 2000 tissue processor 3) 6 foot fume hood (1/2 of base cabinet is flammable storage) 4) 2 Well Diamond wire saws (model 4240) 5) Exakt grinder all of the equipment was being used right up to the last week we closed the lab -- Cathy Mayton ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Superfrost Plus Slides
Hi Claire, We experienced problems with our IHC staining the fall of 2008 and it was traced back to a problem with particular lot numbers of the Superfrost slides. It was difficult to track down due to the inconsistency of the problem and the variation in the problem. We finally spoke with our Ventana technical specialist and she was able to tie our problem to problems that other facilities were experiencing. You could try contacting your Ventana technical resource person; they may be able to help. One thing we found out was that the Superfrost slides are manufactured at one site and distributed to vendors under their own name. Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fahy, Claire Sent: Tuesday, March 16, 2010 4:05 AM To: Lynn Lee Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Superfrost Plus Slides Hi Lynn Yes, our instrument is decontaminated and cleaned as per manual. There was some old threads on Histonet where some people had got varying staining between runs so I was wondering if anybody had found a solution to this problem.Maybe it is simply the slides? -Original Message- From: Lynn Lee [mailto:lynnlee2...@live.com] Sent: 16 March 2010 10:43 To: Fahy, Claire Subject: RE: [Histonet] Superfrost Plus Slides You could have contamination in the instrument. Has it been decontaminated quarterly per the instruction manual? I have never heard of uneven staining from plus slides. Date: Tue, 16 Mar 2010 10:16:38 + From: claire.f...@agriculture.gov.ie To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Superfrost Plus Slides Hi I know this is in reply to an old threat but we are experiencing varying and uneven staining of our sections on the Ventana Benchmark.It is not confined to a perticular anitbody and doesn't happen all the time either. We have been told that it may be the slides.We currently use Thermo Scientific (Menzel-Glaser) superfrost plus slides.Does anyone use the Ventana IHC slides? Has anyone has experienced similar problems?If so,what have you done to resolve them. Thanks Claire fahy Lab analyst, Histopatholgy Lab Department of Agriculture, Fisheries and Food The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). An Roinn Talmhaíochta, Iascaigh agus Bia Tá an t-eolais san ríomhphost seo, agus in aon ceangláin leis, faoi phribhléid agus faoi rún agus le h-aghaigh an seolaí amháin. Dfhéadfadh ábhar an seoladh seo bheith faoi phribhléid profisiúnta nó dlíthiúil. Mura tusa an seolaí a bhí beartaithe leis an ríomhphost seo a fháil, tá cosc air, nó aon chuid de, a úsáid, a chóipeál, nó a scaoileadh. Má tháinig sé chugat de bharr dearmad, téigh i dteagmháil leis an seoltóir agus scrios an t-ábhar ó do ríomhaire le do thoil. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Hotmail has tools for the New Busy. Search, chat and e-mail from your inbox. Learn More.http://www.windowslive.com/campaign/thenewbusy?ocid=PID27925::T:WLMTAG L:ON:WL:en-US:WM_HMP:032010_1 Department of Agriculture, Fisheries and Food The information contained in this email and in any attachments is confidential and is designated solely for the attention and use of the intended recipient(s). This information may be subject to legal and professional privilege. If you are not an intended recipient of this email, you must not use, disclose, copy, distribute or retain this message or any part of it. If you have received this email in error, please notify the sender immediately and delete all copies of this email from your computer system(s). An Roinn Talmhaíochta, Iascaigh agus Bia Tá an t-eolais san ríomhphost seo, agus in aon ceangláin leis, faoi phribhléid agus faoi rún agus le h-aghaigh an seolaí amháin. Dfhéadfadh ábhar an seoladh seo bheith faoi phribhléid profisiúnta nó dlíthiúil. Mura tusa an seolaí a bhí beartaithe leis an ríomhphost seo a fháil, tá cosc air, nó aon chuid de, a úsáid, a chóipeál, nó a scaoileadh. Má tháinig sé chugat de bharr dearmad, téigh i dteagmháil leis an seoltóir agus scrios an t-ábhar ó do ríomhaire le do thoil. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Downtime Procedure
It would be good to hear any responses to this question. Last October our entire facility's computer system was down for 2 1/2 days. We do have downtime log sheets that we implemented but no formal procedure. Management is now looking at how the crash was handled and for ways to improve things. The downtime log sheets were very useful but I'm sure there is much more that we could be doing. Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Tuesday, January 19, 2010 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Downtime Procedure Does anyone have specific downtime procedures for when the Anatomic Pathology area computer goes down? The AP area in my facility does not have a downtime procedure and we ran into an issue yesterday when the computer system went down. We processed, on the fly, as it were, but I now recognize our deficiency in not having a formal procedure. Can anyone provide any examples for me to work off of, so that I don't have to recreate the wheel? Thanks for any help! _ Hotmail: Trusted email with Microsoft's powerful SPAM protection. http://clk.atdmt.com/GBL/go/196390706/direct/01/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] maximum block cutting
Does this average include trimming into the block or just the actual cutting of the section? Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM To: histonet@lists.utsouthwestern.edu; histol...@medsurgpath.com Subject: Re: [Histonet] maximum block cutting The productivity of a histotech is completely different with the number of slides a cytotech is allowed to screen daily. In the first case the final product is not affected by an increase of the productivity but in the second it is. A cytotech has to exert judgment while screening and could get easily tired compromising his/her diagnostic ability. Having said that, the cutting range for histotechs is from 5 to 70 blocks/hour with an average of 24 (sample size = 245 histolabs). René J. --- On Wed, 12/9/09, histol...@medsurgpath.com histol...@medsurgpath.com wrote: From: histol...@medsurgpath.com histol...@medsurgpath.com Subject: [Histonet] maximum block cutting To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 9, 2009, 5:07 PM Hi Histonet, I know we have discussed the average blocks histotechs cut per hour, but does anyone know if there is a maximum blocks that can be cut per hour? Or per day? We have regulations for cytotechs who are reading slides for maximum slides they can read per hour and are wondering if there is any similar regulation for histotechs. Thank you all for you help, Katelin Katelin Lester, HTL (ASCP) MedSurg Pathology Associates, Inc. (503) 443-2157 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Automated HE stainers
Have you had any problems with the Coverslipper? We purchased the Prisma with linked Coverslipper last March and have had on going problems with the Coverslipper jamming with error code -Motor ejector error E15. We have contacted Somagen and were told that this is normal. It is happening at least once a day and sometimes more. We love the stainer and if the Coverslipper wasn't jamming we would love it just as much. What do you do with the coverslipped slides? We find that they don't dry as quickly as they did on the older film Coverslipper. We don't have a vented sign out area so they are staying on the carousel until they are mostly dry. Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Monday, October 12, 2009 5:42 AM To: Connolly, Brett M; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automated HE stainers Brett, We purchased the Tissue Tek PRISMA with the attached film coverslipper a year ago and LOVE IT!! Was a little nervous switching from the glass coverslips to the film, but the docs love the slides and we have had no problems. Very easy to use and we like the fact that we can file the slides the same day without having to wait for them to dry. Karen Karen L. Bauer HTL (ASCP) Histology Section Chief Department of Pathology - Luther Hospital Luther Midelfort - Mayo Health System 715-838-3205 bauer.ka...@mayo.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Friday, October 09, 2009 8:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated HE stainers Which one do you like?...procons? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 tel. 215-652-2501 fax. 215-993-6803 brett_conno...@merck.com Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] double immunostaining with antigen retrieval and no antigen retrieval
Dear All, Is it possible to do double immunostaining for one antibody which doesn't require antigen retrieval, and the other one which doesn't need it. Can you do the one without retrieval first, then retrieve and do the other one, or does the retrieval damage the first staining? We know that doing antigen retrieval for antibody that doesn't require it gives us non specific staining. I would appreciate some advice. Thank you very much. Cathy Malcontenti-Wilson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] immuno staining
We were having a similar issue with our IHC staining on the Ventana Benchmark Classic. We were able to track the problem down to the slides. We never found out what was wrong with the slides but as soon as we changed lot numbers the staining issue cleared up. We were able to track down the problem with the assistance of our Ventana technical rep. Through her we were able to find out that several institutions across Canada were experiencing similar staining problems. We were all using the same lot number of slides. She also informed me that there was one manufacturer of the plus slides and they were sold under multiple vendors. Good luck with tracking down your problem. Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital Kelowna, British Columbia Canada -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jacobs, Genie Sent: Thursday, July 16, 2009 5:18 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] immuno staining We use Premiere Charged Slides purchased from Cardinal and/or Mercedes for our immuno stains. We recently purchased the Ventana Ultra and really like it. We dual mount the control and patient. We have recently had several cases where the control was positive but the patient was not. We repeated the stain and the patient was positive the second time. We have had issues with washing but have increased the time in the oven Has anyone else had this problem We don't have a lot of variables that are different Thanks for any input The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Tek II- last one
Almost have the lab boxed up but I have 1Tissue Tek II staining tray with 12 staining dishes along with 14 more other staining dishes left. Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue Tek II gone
Thanks for all the inquiries but the Tissue Tek II staining tray and all the extra dishes are gone. Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] hand held pH meter for sale
I have a Fisher Scientific Accumet hand held pH meter for sale. Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] wood slide boxes for sale
I have 5 nice wood slide boxes that hold 100 slides for sale Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HI Downdraft fume extractor for sale
I have a HI downdraft fume extractor for $50 and we will provide carbon to refill the drawer. Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] breaking glass jar/vials for MMA embedded specimens
Yes, the scintillation vials are notorious for shattering in the freezer. However, if you put them in a plastic container with a lid on, the shattered glass will be contained and not all over your freezer. When breaking larger glass containers, again place them in the freezer, remove from the freezer after 20 minutes or so, take the lid off, wrap jar in paper towels and hold the ends closed. Wear glovesand eye protection in case shards of glass escape. Strike the jar with a hammer and unroll the paper towel over a garbage can. Rinse the block in tap water to remove any small shards of glass. This is a great way to vent frustration!! Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] small equipment for sale
2 600 gram balances for sale, both were certified last November, also have certified weight set 1 slide dryer with cover, we used at one time for paraffin sectioning 2 Premiere microscopes, used for QC of GMA sections at the time of sectioning, has 4x, 10x, 20x and 30x and 40x 1 Leica DME dual head microscope has 4x, 10x, 20x and 30x and 40x, used for QC of staining and final QC of slides Cathy Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 5 gal MMA for sale
I have a sealed 5 gallon can of Aldrich MMA for sale for $50 plus shipping. We also have 12 or 13 gallons of Stat Lab Reagent Alcohol. Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] light box for sale
I have a light box for $50 plus shipping and a nice Shandon paraffin embedding center. Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] glassware + Tissue Tek II staining trays
I have glass dishes, glass staining racks, beakers, flasks and 3 Tissue Tek II staining trays. I also have lots of the slide holders for the Tissue Tek II staining trays. Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 www.wasatchhisto.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] paraffin slides for sale
Fisher Scientific slides @ 2 $2/box 20 boxes green 5 boxes yellow Premiere brand 20 boxes green 8 boxes white Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 www.wasatchhisto.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] plastic slides for sale
For those who prepare specimens for ground histology we have plastic slides available. Since I am closing my lab we will no longer be selling custom plastic slides. We are closing our stock at half price. 2 x 3 x .030 clear800 slides 2 x 3 x .060 clear64 slides 2 x 3 x .060 white769 slides 2 x 2 x .060 clear88 slides 2 x 2 x .060 white 112 slides higher quality plastic slides 2 x 3 x .030 clear346 slides Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] more plastic slides
found some more plastic slides 2 x 4 x 060 clear164 slides These we used to true the head on the Exakt grinder. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Isocut cutting fluid
Free to good home 6 bottles of 950 ml Buehler Isocut cutting fluid This was sent to us accidently by Buehler and we never used Just pay shipping and it is all yours Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] cassette storage/cardboard slide holder for sale
For Sale 2 set of paraffin block drawers $25 20 or better cardboard slide holders (holds 20 slides) $1 each Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] For Sale small lab equipment
Dear Fellow Histonetters, I have 2 balances for sale 600 gm capacity, top load, each pass and are recertified every year balance weights for standarizing 3 Tissue Tek II staining trays and extra staining dishes and lots of Tissue Tek II staining racks 1 large glass dessicator 1 medium glass dessicator lots of consumables Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Sanderson's Rapid Bone Stain
Dear Fellow Histonetters, There are 3 counterstains that can be used with the Sanderson's Rapid Bone Stain (RBS). We primarily used the acidified acid fuchsin, however, we had clients that only used a van Gieson counterstain. Light green can also be used. We normally stained for 1 minute ground sections in the RBS, rinsed in water and then counterstained. As Gayle mentioned, intensity of the RBS or the counterstain can be increased with addtional time. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] free Tissue Tek II vacuum infiltrator
I have an old Tissue Tek II vacuum infiltrator free to a good home. Only cost will be shipping via ground..either FedEx or UPS. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cathy Crumpton is out of the office.
I will be out of the office starting Mon 03/02/2009 and will not return until Thu 08/27/2009. If you have any histology concerns please route them to Connie Basinski during my absence. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] uncertified histotechs
wow! I've been sitting here reading these opinions and had to get my 35 years worth. I was a histotech long before I was a certified histotech. After 25 years of doing histology, I decided to get my certification for my own gratification, however in doing so it also made quit a difference in my pay scale but did not make me a better histotech as I feel my experience in the field was worth more than that piece of paper. I will put my slides up against anyone else, the ones before my certification and after. I have in the last 2 years had a cytotech who I have crosstrained in histology and she can put out a pretty good slide also. I do not know if she will take the certification or not, but she can work in my lab anytime. I have also in the last 2 years started doing IHC's. I hope to take my certification in IHC sometime in the next 25 years, however, my pathologists are quite pleased with my work. Cathy Boyd HT(ASCP) Beaufort County Medical Center Washington, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] using patient blocks for positive IHC controls
Does anyone know if there are any regulations on how much tissue you can cut from a patient block? We currently use known positive patient slides for our IHC positive control slides? One of our pathologist questioned if we could use an entire patient block ,cutting through the block for controls as long as the slide was still positive. Or is this an individual institution decision? She was especially concerned about CAP. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
