from:"Dorothy Hu"

[Histonet] Tissue Adherence Issue

2019-07-18 Thread Dorothy Hu via Histonet

>
>  2. Tissue Adherence Issue (Knutson, Deanne)
>

Hello,

I think your testing tissue might be over or under fixed in formaldehyde
fixative?
You didn't tell what kind of tissue though. We deal with bone tissue which
needs extensively bake (at 37, 46 degree over night and 58 one hour) and
carefully handled. Superfrost Plus slides are used. Especially gentle heat
for heat induced antigen retrieval. Try to use enzyme antigen retrieval as
possible.
Best luck.

Dorothy Hu



> Message: 2
> Date: Wed, 17 Jul 2019 09:21:49 -0500
> From: "Knutson, Deanne" 
> To: "'histonet@lists.utsouthwestern.edu'"
> 
> Subject: [Histonet] Tissue Adherence Issue
> Message-ID:
> <
> 1e0e2b14c709174b8ac2be0ae7f768330159b5756...@exchange2k7.staprimecare.org>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Fellow Histonetters -
>
> I would appreciate your feedback on an intermittent issue that has shown
> up in our lab.
> All of a sudden, we are having difficulty with tissue specimens falling
> off of our slides on the IHC stains and special stains sporadically.
> About a year ago, we switched instruments on the IHC bench from the Leica
> BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer.
> No adherence issues with all of the validation slides that were run.
> We have tried various types of slides recently as well, and the issue
> still prevails.
>
> Would any of you mind telling me your slide workflow - type of slide used,
> gelatin or not used in flotation bath, how long slides are cooked, etc
> for your IHC slides, for your special stains slides, and even for your H
> slides.
> I would welcome your suggestions and feedback.
>
> Thank you so much!!!
>
> Deanne Knutson
> Supervisor
> Anatomic Pathology
>
>  [X]
>  "Let All Be Received as Christ."
>
>
>
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Re: [Histonet] [EXTERNAL] Deplastifying MMA

2018-02-16 Thread Dorothy Hu via Histonet

Hi Lori,

Would you please tell me which company and catalog # of  tetrahydrofuran
you used before?
For how many minutes you did for deplastifying in tetrahydrofuran?
Thanks in advance.

Dorothy

Message: 3
Date: Wed, 14 Feb 2018 20:05:16 +
From: "Garcia, Lori, M.Sc." 
To: "reuel.corne...@tsrh.org" 
Cc: "histonet@lists.utsouthwestern.edu"

Subject:
Message-ID:


Content-Type: text/plain; charset="us-ascii"

Hi Reuel,

One thing you could try is THF (tetrahydrofuran). We have used it in the
past to rapidly dissolve the MMA on slides.

Good luck,
Lori
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[Histonet] Deplastifying MMA

2018-02-14 Thread Dorothy Hu via Histonet

>
> Hi  Reuel
>

We had the same thing as you. It was caused by bad MMA, I tested. Write
down the lot # and ask refund. Test new lot # 1st of MMA in the future. It
was very frustratingI know.
Best luck.

Dorothy





> Message: 2
> Date: Mon, 12 Feb 2018 21:07:15 +
> From: Reuel Cornelia 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] Deplastifying MMA
> Message-ID:
>  namprd17.prod.outlook.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello histonetters,
>
> I have a problem with my MMA plastic section on my slide. I could not
> remove the plastic MMA even if I leave the section slides in Xylene for
> several days with heat incubation at 60 degrees. This is the first time
> that happened to my section for several years of doing the same thing over.
> I was thinking that it was my Xylene lot number so I tried to change it but
> still does not remove the plastic, and then Itried different solvent
> Acetone, and Ethylene glycol Monoethyl ether but still it does not work.
> Can I ask for help if anyone knows what was going on and what would be the
> best way to remove this plastic from my section?
>
> Just to give you my embedding solution was MMA -94% , dibutly phthalate-
> 5% and perkadox 16- 0.5%. This have been my solution for years and I do not
> have any problem with the removal of plastic section until now. I was
> thinking that my MMA (M55909) different lot number from Sigma Aldrich may
> have cause this because even I tried to use the MMA to dissolve my plastic
> it does not work.
>
>
> Thank you for and any opinions or protocols are greatly appreciated.
>
>
> Reuel Cornelia
>
> TSRH
>
> 214-559-7766
>
>
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[Histonet] EDTA decalcification tissue issues

2017-11-13 Thread Dorothy Hu via Histonet

We didn't compare EDTA with formic acid. But heard that formic acid also
can do many IHC and ISH, if not all the antibodies and all probes. I always
have problem of bone falling off, no matter what kind of slides. Thinking
both PFA and EDTA affect on this issue. So trying frozen tape unfixed,
undecalcified bone now. If anyone has research paper regarding this, please
share.
Thanks.

Dorothy Hu
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[Histonet] Cut FFPE Slides for ISH

2017-08-28 Thread Dorothy Hu via Histonet

Hi Jennifer,

My ISH slides (FFPE) were stored in -20oC for several months or more than
one year and the targets were less abundant. I didn't use RNAscope though,
which is more sensitive. I don't know what kind of RNA your researcher try
to locate and need to do ISH within 24 hours after cutting?  Fresh cut
section will yield more signal for sure. I think as long as you have a few
copies of genes in your tissue, RNAscope should be able to detect.

Dorothy Hu
HSDM







> Message: 2
> Date: Mon, 28 Aug 2017 15:30:08 +
> From: "Jennifer  Phinney" <jh...@vet.k-state.edu>
> To: "Histonet@lists.utsouthwestern.edu"
> <Histonet@lists.utsouthwestern.edu>
> Subject: [Histonet] Cut FFPE Slides for ISH
> Message-ID:
> <BN3PR0501MB134800650CC628A35D07DD36B39E0@BN3PR0501MB1348.
> namprd05.prod.outlook.com>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Histonet,
> I am hoping someone can point me in the right direction to find some
> references for how long FFPE slides intended for ISH can be kept before
> staining.  I have a researcher who swears their slides have to be stained
> within 24 hours of being cut, which does not sound correct to me.  I've
> used ACD's RNAscope before and their protocol lists the timeframe as 3
> months (so this is a bit of a difference).
>
> There's a lot of information about fixation times, but I've come up empty
> handed for cut slides.
>
> Thanks everyone!
>
> Jennifer Phinney QIHCCM
> Project Coordinator
> Kansas State University
> Veterinary Diagnostic Lab
>
>
>
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[Histonet] KOH softener to "decalcify" bone?

2017-05-08 Thread Dorothy Hu via Histonet

I know someone used KOH with EDTA together to decalcify bone.
It is worthwhile to try KOH only to soft bone and kept dynamic labeling.
If anyone had tried successfully, please let us know.
Thanks.


>
> Today's Topics:
>
>1. Fabric softener to "decalcify" bone? (Angela Lamberth)
>2. Re: Fabric softener to "decalcify" bone? - Downy has  formic
>   acid?? (Angela Lamberth)
>
>
>
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Re: [Histonet] Histonet Digest, Vol 157, Issue 13 Kawamoto's film method (Jamie McGinness)

2016-12-18 Thread Dorothy Hu via Histonet

Please check this web for the answer of your question.
http://section-lab.jp/English/Referece%20E.htm

Dorothy Hu
>
> 
> I am looking into trying the Kawamoto's film method. ?I have the order sheet 
> for the cryofilm sheets and other supplies. ?I did have a few questions.
> 1. ?I was wondering if anyone knows when you order a sheet how many slides 
> can be produced from that sheet? ?2. ?Does anyone know what the transfer film 
> is??3. ?What are the advantages to using their SCCM mounting medium ?and the 
> difference between the four types offered G1, R1, R2, R3?4. ?What are the 
> advantages of using heir SCEM embedding medium and the difference between the 
> SCEM and SCEM L1? ?5. ?Does anyone know the differences of the cryofilm 
> 2C(9), 2C(10), and 3C(16UF)?
> Thanks so much for any information you .
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Re: [Histonet] cryosectioning undecalcified samples (Caroline Miller)

2016-03-04 Thread Dorothy Hu via Histonet

Hi,

I compared cryojane with Kawamoto's method. I prefer the later. Please see
the following paper.


Arch Histol Cytol.  2003
May;66(2):123-43.
Use of a new adhesive film for the preparation of multi-purpose
fresh-frozen sections from hard tissues, whole-animals, insects and plants.
Kawamoto T

1.

Good luck.

Dorothy
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Re: [Histonet] Histonet Digest, Vol 147, Issue 21"............Double stain IHC question

2016-02-20 Thread Dorothy Hu via Histonet

I wonder if streptavidin Alexa (495 or 488) can be use to bind to biotinlyted 
primary. So no worry about species issue? May need to do biotin blocking first 
to get ride of endogenic biotin I guess. 

Dorothy Hu

Sent from my iPad

> On Feb 20, 2016, at 1:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote:
> 
> Double stain IHC question

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[Histonet] Decalcification with formic acid sodium citrate

2015-07-26 Thread Dorothy Hu via Histonet

There was a paper 
http://www.genedetect.com/Merchant2/ExampleRefs/Decalcifying_protocols.pdf
Talking about formic acid (Morse solution) can get as good result as EDTA in 
ISH. 
FYI.

Dorothy Hu


 Today's Topics:
 
   1. Re.  Decalcification with formic acid sodium citrate
  (Gayle Callis)
   2. Re: Re. Decalcification with formic acid 
 
 Merissa and Tim,  
 
 
 
 This formic acid decalcifying solution is basically the classic Evans and
 Krajian fluid (Sheehan and Hrapchak,   Theory and Practice of
 Histotechnology, 2nd edition, P.92).  Shandon has added other ingredients
 for some reason, and has kept those concentrations proprietary.   You really
 don't need to add a surfactant or PVP emulsifier when making up this
 decalcifying agent.   Simply use the classic recipe for successful
 decalcification.   This is also referred to as buffered formic acid and in
 some publications an acidic buffer.  It is excellent if IHC is needed and
 less damaging, obviously, than a strong mineral HCL acid decalcifiers.  
 
 
 
 Sodium citrate crystals (a buffering salt) 10 g 
 
 90% formic acid stock25 ml  
 
 Distilled water75 ml   
 
 
 
 One can calculate the concentration of formic acid i.e. approx. 4.5% since
 is it made from 90% formic acid stock.  
 
 
 
 Don't bother with the surfactants or PVP.  
 
 
 
 Enjoy an excellent in house formic acid decalcifying solution.  I also
 suggest you read Sheehan and Hrapchak textbook chapter on bone as a way to
 familiarize yourself with decalcifiying solutions that manufacturers now
 supply with some modifications.  Some manufacturers will refer to these
 methods but probably prefer not to do this since they want you to buy their
 commercial product that is obviously a time saver with elimination of having
 to store stock acid solutions.   The classic methods made in house are
 excellent if you have time to make them up.   Formic acid with sodium
 formate is another popular buffered formic acid.   I suggest you look for
 another source/manufacturer of the your favorite decalcifier in question as
 more than one company will make it.  Decal Corp, recently sold to Stat Lab,
 could also be the source as Shandon isn't the only game in town.   Others
 are Newcomer Supply, Poly Scientific.  Not having to make it up may remain
 your preference. 
 
 
 
 Gayle M. Callis 
 
 HTL/HT/MT(ASCP) 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 Written by Tim and Merissa:   
 
 
 
 Merissa,
 
 
 
 Water77-80   solvent
 
Formic acid  21-23   active ingredient
 
 Fluorad  1   surfactant  - a
 wetting agent to make the solution wet the bone more easily
 
Sodium citrate   1   emulsifier , buffer
 
 Polyvinyl pyrrolidone1   emulsifier 
 
 
 
 They say less than one percent of the last three, but you really have no
 idea whether that is 1%, .1% or .01%. It could be any of those.
 
 
 
 But all those surfactants and emulsifiers are meant to keep the solution
 viable for long periods on the shelf. When you make it fresh you don't
 really need them.
 
 
 
 You can either buy a different decalcifier, or make your own. Making your
 own with just the water and acid will work just fine. 
 
 
 
 
 
 Tim Morken
 
 Pathology Site Manager, Parnassus 
 
 Supervisor, Electron Microscopy/Neuromuscular Special Studies
 
 Department of Pathology
 
 UC San Francisco Medical Center
 
 
 
 -Original Message-
 
 From: M.O. via Histonet [mailto:
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet histonet at
 lists.utsouthwestern.edu] 
 
 Sent: Wednesday, July 22, 2015 1:24 PM
 
 To:  http://lists.utsouthwestern.edu/mailman/listinfo/histonet histonet at
 lists.utsouthwestern.edu
 
 Subject: [Histonet] understanding reagents in decalcifier; making it
 in-house
 
 
 
 Hello Histonet
 
 
 
 The supplier for our decalcifier, TBD-2 from Shandon, is having issues with
 getting the product out and we will not be receiving it for at least another
 month.  Our samples are piling up and I don't know what I should do, but
 maybe I can make the decalcifier in-house.  I am wondering if I can make my
 own based on the reagents they listed and their percentages and if certain
 reagents are not actually necessary.
 
 
 
 The samples we typically decalcify are mouse knees (decal time = 2 days),
 mouse spines (3 days), human bone slabs about 7mm in thickness (7-12 days).
 Fixation is in zinc buffered formalin, then decalcification, then 70% EtOH.
 Our choice to use TBD-2 is due to the gentle decalcification for IHC and we
 get GREAT results.
 
 
 
 Composition of Shandon TBD-2 Decalcifier:
 
 ComponentWeight %
 
 Water  77-80
 
 Formic acid 21-23
 
Fluorad   1
 
 Sodium citrate   1
 
 Polyvinyl pyrrolidone

[Histonet] TRAP Stain Help

2015-05-12 Thread Dorothy Hu

If you include a positive slide to your TRAP? It is a must for each run. It
could be many things if you didn't have positive slide, will be hard to
troubleshot. Also if you see any pink color befor
counterstain/coversliping? If do, there may be because of decolored by
ethanol/xylene and mounting media. You have to use water based mounting
media.
I don't use Sigma kit (tried once), but my protocol is more sensitive. We
do trap on both paraffin and MMA sections. If you need I can forward you
the paper.

Dorothy Hu
HSDM


Message: 2
Date: Mon, 11 May 2015 20:26:14 +
From: Wait, Trevor Jordan wa...@livemail.uthscsa.edu
To: Histonet@Lists. Edu histonet@lists.utsouthwestern.edu
Subject: [Histonet] TRAP Stain Help
Message-ID: 1431375976414.44...@livemail.uthscsa.edu
Content-Type: text/plain; charset=iso-8859-1

Hey guys, recently I've used the Sigma Aldrich TRAP Stain Kit in order to
stain for Osteoclasts in Formalin Fixed Paraffin Embedded bone tissues that
are EDTA decalcified.  Unfortunately there was no TRAP stain to be found
whatsoever on all slides that I stained.  I was hoping that someone with
some experience with TRAP stain could really help me out!  Here are a
couple of reasons that I have wondered as to why the TRAP stain might not
be visible

1. Staining too long with Hematoxylin counterstain?  I have noticed in
several trial runs that sometimes if the Hematoxylin counterstain is too
long then this can effect the amount of TRAP stain that shows up with the
osteoclasts.  Perhaps I'm just over analyzing

2. Perhaps no osteoclasts were present to even staindoes anyone know
what the usual ratio of Osteoclasts to Osteocytes are?  In the past stains
that I have usedwhenever there is an osteoclasts that shows up...it is
usually pretty spotty and many times they are in groups together...is this
how it normally is?

3.  Perhaps the TRAP stain is being washed away through the rinsing with dI
or dehydration with ethanol/clearing just before mounting.


A protocol that I used before the Sigma Aldrich Kit incorporates incubating
the slides in 37 celsius water for 1 hour just prior to allowing the slides
to sit in the TRAP stain solution (this is also 37 celsius in the same
water bath) and letting it sit for 20 minutes.  With this stain...there
seems to be a consistent showing of osteoclasts but I'm just not sure if
all of the osteoclasts are showing up correctly...that is why I moved to
the Sigma Aldrich kit to make sure and the results didn't show ANY
osteoclasts there!  Anyways...I'm just curious if there are any reasons
that the TRAP stain is not showing up...I would appreciate any input!




Trevor Jordan Wait
University of Texas Health Science Center, San Antonio
Class of 2017 MD Candidate
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[Histonet] Bubble in MMA blocks

2015-05-01 Thread Dorothy Hu

Hi histonetter and specifically Gayle Callis,

I try to explain why we need to leave MMA embedded glass vials in water
bath during embedding to a research lab. But I need experts to have a
convencing explaination. The lab use vacuum desiccator (which has sealed
ring to keep vacuum) to leave the vials from 4 degree overnight, remove it
to room temperature for 3 hours, then move to 32 degree oven with the vials
in the vacuum dessiccator. They often found there are bubbles in the vial
after polymerization down. I knew they have change the way to do embedding
by remove the vial from dessiccator to close the cap and leave the vial in
water bath and space out to vent the heat generated during polymerization.
But how can I say the vacuum dessiccator.
Thanks everyone to explain.

Dorothy Hu
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[Histonet] Decalcification using EDTA

2015-04-24 Thread Dorothy Hu

It is optional to decal in 4oC, but it is much better for preserve
antigenicity and get good result from enzymatic staining.
Similar way as you do fixation, you can choose RT or 4oC.
Dorothy Hu


Hello Histonetters

Is it imperative OR optional to maintain samples at below 4 degrees celcius
when using EDTA to decalcify bone samples

Thank You
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[Histonet] mice tibia histology

2015-04-21 Thread Dorothy Hu

Your decal time should be according to your mice age. Four weeks old mouse
normally need four changes of EDTA in two weeks time.
I think your process time is a bit too long. If your four weeks old mouse
tibia, 13 hours from 70% ethanol to last infiltration paraffin should be
fine. Your shrinkage is because 1. temperature too high?; 2. too long in
xylene?
hope this help.
Dorothy Hu


Hi does any one doing mice tibia histology, we use to fix in formalin for
24 hours and transfer in 2and half weeks in 0.5M EDTA three change and
process for 24 hours long protocol in automatic processor.
But I am facing problem with bone marrow shrinkage. If any one have idea
for decalcification timing and solution can resolved this problem and keep
bone marrow intact with bone.
Thank you in advance.

Regards,
Shruti
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[Histonet] formic acid treatment and alpha-synuclein staining (Tyrone Genade)

2015-04-13 Thread Dorothy Hu

I used 88% formic acid in Dwater to pretreatment of mouse tissue slides
before beta-Amyloid and Anti-Glial Fibrillary Acidic Protein (GFAP)
antibodies IHC. 10 minutes in formic acid with gental shaking before
blocking step.

You may also check this paper.

Acta Neuropathol.
http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=C-terminal+alpha-synuclein+immunoreactivity+in+structures+other+than+Lewy+bodies+in+neurodegenerative+disorders.#
2000 Mar;99(3):296-304.
C-terminal alpha-synuclein immunoreactivity in structures other than Lewy
bodies in neurodegenerativedisorders.
Takeda A
http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Takeda%20A%5BAuthor%5Dcauthor=truecauthor_uid=10663973
1, Hashimoto M
http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Hashimoto%20M%5BAuthor%5Dcauthor=truecauthor_uid=10663973
, Mallory M
http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Mallory%20M%5BAuthor%5Dcauthor=truecauthor_uid=10663973
, Sundsumo M
http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Sundsumo%20M%5BAuthor%5Dcauthor=truecauthor_uid=10663973
, Hansen L
http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Hansen%20L%5BAuthor%5Dcauthor=truecauthor_uid=10663973
, Masliah E
http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Masliah%20E%5BAuthor%5Dcauthor=truecauthor_uid=10663973
.

Hope this wil help.

Dorothy Hu
HSDM

Message: 3
Date: Fri, 10 Apr 2015 15:00:55 -0500
From: Tyrone Genade tgen...@gmail.com
Subject: [Histonet] formic acid treatment and alpha-synuclein staining
To: histonet histonet@lists.utsouthwestern.edu
Message-ID:

caeyee3ma4xhp0_6brsqzqpujsohi15vdnzn+vkbrbb2pfws...@mail.gmail.com
Content-Type: text/plain; charset=ISO-8859-1

Hello,

I'm do some research on alpha-synucleinopathies and how to stain them. I am
reading, from the Braak et al papers, that the slides need be treated for
10 minutes with formic acid. The method is largely absent. I'm a biochemist
so I understand the formic acid is denaturing and unfolding the aggregates
and exposing the epitopes. Would this effect the ability to label other
epitopes such as plaques and tangles, tyrosine-hydroxylase, FOX2A etc..?
Does anyone have any experience in double-labeling sections for Lewy Bodies
and other proteins?

In the same paper an alternative method of a 48 hr incubation on the slide
is suggested. Details of the staining buffer are absent but I'm hunting
them down and expect to find something like Triton X-100 in it which will
again unfold the aggregate (a little).

Does anyone have a reliable protocol to share? I'm now several references
deep* from my original article and am still trying to find the paper that
describes the method.

Thanks
--
Tyrone Genade

*Back in my Biochemistry days we played a game: Hunting for Bradford.
Essential, select a paper at random, see if it uses the Bradford Assay and
find out how many papers you had to read before arriving at the original
paper by Bradford. My then Prof held the record at some 30+ papers...

Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org

Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.

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[Histonet] Kawamoto methods

2015-02-11 Thread Dorothy Hu

Try this http://www.section-lab.jp/.  Just email them and ask how to order.

Dorothy
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[Histonet] cutting MMA cross section of mouse femur

2014-12-10 Thread Dorothy Hu

Dear Histonetters,

I normally use 85/15 ratio of MMA/dibutyl phthalate (DP) in my mouse tibia
and femur longitudinal sections. But for cross section of mouse femur. Do
you change the ratio of MMA/DP to make block more soft or hard? It is
difficulty to get good cross sections. Any expert especially from hard
tissue committee, please help. I'm very much appreciated.

Dorothy Hu
MGH endocrine
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[Histonet] Re: Histonet Digest, Vol 131, Issue 26

2014-10-24 Thread Dorothy Hu

*Regarding processors*

*I also need to help on determine which processor for our core. Our
majority tissue is bone. *
*Right now, we like VIP serials because of vacuum/pressure plus agitation.
Please help me*
*on this VIP processor, I never used this kind one. We are very small
volume*
*core, also do frozen and plastic undecal bone. We have a Shandon Excelsior
now and feel the *
*vacuum volume for this 10 years old processor is not enough for bone. *
*Any input for this will be greatly appreciated. *

*Dorothy*
*MGH endocrine histocore*




9. RE: Processors (Joelle Weaver)

   11. Re: Processors (Jay Lundgren)



 Message: 9
 Date: Thu, 23 Oct 2014 19:05:23 +
 From: Joelle Weaver joellewea...@hotmail.com
 Subject: RE: [Histonet] Processors
 To: Jamal j.rowa...@alborglaboratories.com, 'GMail'
 nguy0...@gmail.com,   histonet@lists.utsouthwestern.edu
 histonet@lists.utsouthwestern.edu
 Message-ID: snt149-w792d852db98a977b194067d8...@phx.gbl
 Content-Type: text/plain; charset=iso-8859-1

 Agree


 Joelle Weaver MAOM, HTL (ASCP) QIHC





  From: j.rowa...@alborglaboratories.com
  To: nguy0...@gmail.com; histonet@lists.utsouthwestern.edu
  Date: Thu, 23 Oct 2014 11:25:23 +0300
  Subject: RE: [Histonet] Processors
  CC:
 
  hi colleague
  if your daily processed specimens less than hundred, go ahead for VIP5.
 
 
 
  Best Regards,
 
 
  Jamal M. Al Rowaihi   Anatomic Pathology Supervisor   | Al Borg
  Medical Laboratories |  Mobile +966 503629832|
  j.rowa...@alborglaboratories.com
  Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
  Phone: +966 12 670 0099 | Fax: +966 12 676 4984 |
  www.alborglaboratories.com
 
 
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu
  [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of GMail
  Sent: Thursday, October 23, 2014 12:33 AM
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] Processors
 
  Please help me decide on a processor, I am currently inquiring about
  refurbished processors for a small derm path lab with very low volumes. I
  have quotes for  VIP5 for $24-27k, VIP2000 for $8k, VIP E150 for $14k
 and a
  Leica TP1020 type 4 for $17. Which one would you recommend? What's
 durable
  and won't break down often? Should I go for the vip2000 compared to vip5
 to
  save money?


Sincerely?
   Jay A. Lundgren,
 M.S., HTL (ASCP)




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[Histonet] Protocol of MMA Plastic section TRAP staining

2014-10-22 Thread Dorothy Hu

Dear Histoneters,

Can someone email me your TRAP protocol for MMA section? I have one, but
staining is not consistent. No matter I stain for how long, ~ several hours
sometimes. There are always variations. I sincerely hope I can get
different protocol form you and make change. I remember saw other TRAP
protocol which is much longer than mine.

The protocol I used was:

1. Dissolve 12mg Naphthol AS-MX phosphate (Sigma, N-5000) in 0.5ml N,N
methylformamide (Sigma,D8654).
2. Mix above into 50 ml Sodium tartrate with 0.1M sodium acetate buffer at
pH5.0.
3. Mix 30mg Fast Red Violet LB salt (Sigma, F3381) with above solution and
filter.

Stain slides @37oC for at least one hour. The solution can be use for one
month when stored in 4oC.
Continue to do counterstain and mount slides.
Thank you.

Dorothy Hu
MGH Endocrine histocore
Email: d...@partners.org
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[Histonet] Re: Histonet Digest, Vol 130, Issue 16

2014-09-18 Thread Dorothy Hu

Hi Dear Histoneters,
Can you tell me which kind of Brady labeler you are using? I want to get
one. Do you have catalog number? Many thanks.
Dorothy
MGH
Endocrine histocore



 I use the Brady system and I like it a lot.  I work in a small animal
 diagnostic lab and will have 60-80 blocks on most days.  It saves me time
 and even better I don't have to read my writing in the morning.  I use it
 for the cassettes and slides mainly but I label reagents I mix with it to.
 I find it very versatile.
 Roberta Horner HT/HTL
 Animal Diagnostic Lab
 Penn State University


 Subject: [Histonet] Slide and cassette printers?

 What are all of you using these days?  What would be good for a small
 histo lab that has the potential to grow?  (I am already aware of what
 Leica and Thermo have from the archives here, but I am still interested in
 your opinions, of course.)  Has anyone tried the printers, labels and
 attachment machine from Brady?

 Thank you all so much,
 Kathleen


 Principal Lab Technician
 Histopathology Lab
 Office of Translational Sciences
 Rutgers, the State University of NJ
 41 B Gordon Road
 Piscataway, NJ 08854
 (848) 445-1443
 FAX (732) 445-6905



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[Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone?

2014-01-31 Thread Dorothy Hu

which company do you ordered MMA?
Sigma has back order, so I try to find reliable resource here.

Thanks,

 Dorothy
abt...@gmail.com
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[Histonet] Help on get PDF file paper from J. Histotechnology

2014-01-17 Thread Dorothy Hu

Hi Histonetters,

Would you please let me know why I can not get PDF file from J
Histotechnology any more?

I renewed my membership after new year and changed my password since i
don't remember  my old one. I can get in Maney Online for only Abstract,
but not whole article.

The paper I tried to get is:
Parallel experience of two different lab with initiator perkadox 16 for
polymerization of MMA.
Vol 17, No 4, pp.343-348.

Thanks in advance.

Dorothy
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Re: [Histonet] Help on get PDF file paper from J. Histotechnology