Re: [Histonet] New York Licensure
They will need to take the HT and have the results sent to NYS for their license. At this time NYS does not recognize the HTL exam for licensure eligibility. Jen Campbell Sent from my iPhone On Jun 18, 2013, at 10:46 AM, Mayer,Toysha N tnma...@mdanderson.org wrote: Good morning, This question is for those in the New York state area: What is needed for an HTL to become licensed to work in New York state. I have two students who are moving to the area after graduation (August), and will be eligible to sit for the ASCP HTL. The problem is the license requirements are listing HT as the qualifying certification. One student has contacted a recruiter and the state licensure agency and still is not sure what to do. The state society said that since there are no HTL programs, a HT exam would have to be passed. Huh??? The students are moving from Texas to New York state, and will hold a BS HTL, eligibility date for BOC of Aug 15. Any help would be appreciated. Toysha N. Mayer, MBA, HT (ASCP) Instructor, Education Coordinator Program in Histotechnology School of Health Professions MD Anderson Cancer Center (713) 563-3481 tnma...@mdanderson.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
We use India ink and then use 3% acetic acid to affix it to the specimen. Works great on ellipse excisions as well as small slices. Jen Campbell On Wed, Mar 6, 2013 at 10:49 AM, Dermpath Lab d...@att.net wrote: Hi! I was just wondering what different facilities use for red ink/dye. Some of the new ones we have tried gets too light or washes off during processing. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, BS, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] troubleshooting help
We have seen that if our paraffins were not properly rotated. On Mon, Feb 11, 2013 at 10:09 AM, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: I am having trouble with this scenario that I have walked into today. Our Sakura VIP 5 tissue processor had about 150 cassettes on it and they are all overprocessed, very hard. I have checked with a hydrometer all of the alcohols and all seem fine and in the proper order. There were no error codes, nothing stopped in any given step, they were done on time. The only thing that is unusual is that when the tech opened up the processor, she noticed the top of the lid had a large amount of paraffin hanging from it, which we do not normally see. I checked the carbon filter, it is fine. Anybody out there have any ideas as to what could be going on ? Much thanks for whatever advice I am given. I have never in all of my years come across a scenario such as this without there having been alcohols incorrectly placed. Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, BS, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Metal molds
We clean ours in the VIP every Friday. We recycle our xylene substitute from the VIP but you cannot recycle the cleaning alcohol. They are all bright and shiny for Monday morning! Jen Campbell On Wed, Oct 10, 2012 at 9:46 AM, Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov wrote: Does anyone that uses the VIP to clean their base molds also recycle the alcohols/xylene? We were told that you should not clean the molds in the processor if you were recycling. Thanks! Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harris, Diana Sent: Wednesday, October 10, 2012 9:42 AM To: 'susan.wal...@hcahealthcare.com'; joellewea...@hotmail.com; valerie.han...@parrishmed.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Metal molds At our lab we clean the molds in the VIP cleaning cycle then dip in mold release also. Works well. The only precaution is to drain the molds well after dipping otherwise embedding can be effected. Diana Harris QC Method Development Technologist Dept. Of Laboratory Medicine Anatomical Pathology Royal Jubilee Hospital Victoria, BC Canada -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of susan.wal...@hcahealthcare.com Sent: Wednesday, October 10, 2012 12:01 AM To: joellewea...@hotmail.com; valerie.han...@parrishmed.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Metal molds We put our molds in the VIP before running the cleaning cycle daily. Then we dip them in alcohol containing mold release..air dry and store. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, October 09, 2012 3:27 PM To: valerie.han...@parrishmed.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Metal molds I always cleaned them daily, either the very hot water, soapy water method, with water running over them in the sink with them on their sides so it passes over them, not upright so the water sits in them- then a rinse in alcohol and completely air dry. Or you can always do the clean cycle with the racks, running them through xylene, etc. They come out very clean this way- used an old processor that was a backup for this most of the time. But I always did them daily, but also wiped each one out with gauze if I used them twice in an embedding session ( for more than one specimen in that large batch). Also I like metal, I hate those plastic ones. If you keep the block face surface of the mold warm-hot, and flatten before it turns completely white the specimen is at the surface and you are able to see the edges easily without a lot of facing. I think this saves time cutting through paraffin, and saves blades. Plus if the specimen is not flat enough, you see it right away and know if you must re-embed to get a complete, representative section, rather than after you have cut some superficial parts of some edges away and not others, only to have to re-embed anyhow. The other problems I see are when people are afraid of big molds- please if you are only taking one section, use one large enough to leave a perimeter. Don't try to squeeze it into a medium mold, you are unlikely to need multiple sections on one slide and it is much easier to get flat and get a good section. Also please put enough paraffin on top, so that when it is cool the layer over the grooves in the cassette is not so thin that you can clearly see the depressions. That little bit of paraffin is much cheaper than tech time in re-embedding and fussing with a block longer than you should. Not so much a big issue for many specimens, but anything hard/ dense, such as bone, cervix, uterus, leeps, ( you get the idea) it is not anchored enough without a good dose of paraffin, causing more chatter when you section, and maybe chipping out more frequently, or even the whole bottom surface to lift off the cassette. I guess I have some pet peeves with this topic, so thanks for letting me get that out! Joelle Weaver MAOM, HTL (ASCP) QIHC From: valerie.han...@parrishmed.com To: billodonn...@catholichealth.net; histonet@lists.utsouthwestern.edu Date: Tue, 9 Oct 2012 10:51:01 -0400 CC: Subject: [Histonet] RE: Metal molds We clean our molds once a week. Soak them in Xylene to remove paraffin, soak in 100% alcohol to remove xylene, rinse in running water, dry and spray with mold release solution. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506
Re: [Histonet] Sakura glas coverslipper and mounting media types
ClearMount from American MasterTech. Speed and volume set to 3. We put our slides overnight in 60 degree oven prior to filing. On Thu, Sep 13, 2012 at 12:58 PM, Cathy Crumpton cathy.crump...@tuality.org wrote: For those with the Sakura Glas coverslippers, what type of mounting media are you using in these? Sakura recommends only their brand of course, but I want something that dries faster. We do not have excess mounting media on the outside of our slides, but after being in the oven for three days we had several slides sticking. It looked like the mounting media slipped down the slide to pool at the bottom and stick. I am thinking their media is too viscous. If I turn the amount down on the machine any further we end up with not enough media on the slides and get large air pockets after just a few days of storage. BTW the oven is at 40 degrees and isn't boiling hot to dry the slides. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Eosin
What clearing agent are you using? If it is a xylene substitute then you might want to rotate or change the alcohols after eosin after a few racks of slides. We use ProPar on our stainer and rotate our alcohols after eosin after every 3 racks that have gone through. On Thu, Aug 9, 2012 at 11:23 AM, Hannen, Valerie valerie.han...@parrishmed.com wrote: Hi Folks...I am hoping you all give me a little help. Our Pathologists are complaining about our Eosin on the HE's being weak. The funny thing is, is that it can go one day to the next...one day it looks great...the next it is weak!! I have already done some experimenting with...1) time tissue spends in Eosin 2) making sure that the alcohols after Eosin are the proper concentrations3) reducing the time that the tissues spends in the alcohols atfer Eosin... I have even gone as far as 4) increasing the rinse time in water after the decolorizing and bluing steps. 5) I have checked the pH of the water as well. Any help and suggestions would greatly appreciated!! Thanks Gang!! Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com * This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Teabags
We use actual teabags that we purchase in bulk. We filter the contents of our specimen bottles but instead of filtering into the teabag we make a cone-shape and filter onto the teabag and then neatly fold it to fit in a cassette. We are a derm lab so some of the shave biopsies we receive are curled. Once the pieces are cut at grossing we place them on a wet teabag and again neatly fold the teabag and place it in cassette. At embedding we open them on the warm area of the embedding center and don't have issues. The key for us is we put everything on the teabag not in it. Hope this helps! Jen Campbell On Fri, Aug 3, 2012 at 1:39 PM, Contact HistoCare cont...@histocare.comwrote: Hi all, Just a curiosity of mine, having contracted for many places I've seen many different processes, some efficient and some inefficient. I find a lot of labs do what they've always done just because they've always done something a certain way for so long whether it's useful or not and generally are not interested in change. One of these things I'm referring to is using teabags. I know some of you LOVE them, but there are few things I loathe more than trying to dig out a tiny biopsy sample from a teabag along with trying to open it while being stuck together by the wax. Why in the world would anyone ever use teabags when there are microcassettes and even biopsy cassettes? Please let me hear it. www.HistoCare.com Histology Staffing for your Lab ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding beads
The identifier(whatever is using to distinguish who did the task) is not embedded with the specimen. It is put in the top portion of the cassette. At least that is what we have always done here. On Mon, Jul 2, 2012 at 1:17 PM, Amber McKenzie amber.mcken...@gastrodocs.net wrote: How do you not cut thru the bead when sectioning? I'm intrigued by this process b/c I've never heard of this before. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kelly Boyd Sent: Tuesday, June 26, 2012 3:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Embedding beads I started using the embedding beads several months ago as a quick way to track who embedded what cassette. Each tech is assigned a number according to the number on their microtome. I get the beads from Cancer Diagnostics. You can probably find something similar at a craft store. These come in small round plastic containers that fit easily any where on the embedding center. They place the bead in a bottom corner of the cassette when topping it off with paraffin. They have actually saved us time. Once the techs get used to it, it might add a few seconds to the embedding. Before, the techs had to write down which blocks they embedded. (Very time consuming and often not complete). If I needed to see who embedded a certain block, I had to go check that log book. Now I can see who embedded it just by looking at the block. Kelly ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Embedding beads
We use squares of colored construction paper. Each tech is assigned a color and they use those or their initials for all of the different jobs in the lab. There has to be accountability for every job. Jen Campbell On Mon, Jun 25, 2012 at 9:43 AM, Amber McKenzie amber.mcken...@gastrodocs.net wrote: Does this slow the embedder down any having to pick a bead up each time to embed along with the tissue? I've never heard of this procedure before. The labs I've worked in have an embedding log and we initial each case we embed. What do you keep the beads in and are they set in a particular spot on the embedding station so they're easily assessable? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alan Taylor Sent: Friday, June 22, 2012 5:15 PM To: Willis, Donna G.; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Embedding beads Hi All We use Hamma beads to identify individual embedders on a particular day. Hamma beads will be well known to parents and child carers as tiny tube like beads that can be made into many patterns and then ironed (by adults) to make colourful place mats etc for children. Hamma beads come in a very wide range of colours, in bulk bags from any good toy or handicraft store. They are very cheap and they are clearly visible when placed in the corner of an embedding cassette when filling with wax. We have a small staff in our lab so the colours we have chosen are readily identifiable to an individual who has embedded a particular block. I can strongly recommend them, we have used them for a number of years as a traceable guide to a particular block. Hope this is helpful to all of you who are embedding lots of blocks each day. Best wishes to you all Alan Taylor BSc(Hons), FRMS Microtechnical Services 71 Sweetbrier Lane Heavitree Exeter. Devon. EX1 3AJ.UK - Original Message - From: Willis, Donna G. donna.wil...@baylorhealth.edu To: histonet@lists.utsouthwestern.edu Sent: Friday, June 22, 2012 3:58 PM Subject: [Histonet] Embedding beads Does anyone out in Histoland know of a vendor other that Mar Med to get small beads to put in cassettes to designate the person that embeds. Thanks, Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 office ph. 214-725-6184 mobile donna.wil...@baylorhealth.edu ** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] BLock alignment tool
American MasterTech carries it and I believe it was ~$200. Newcomer Supply had it too but I think the price was higher from them. On Thu, May 31, 2012 at 2:48 PM, Vickroy, Jim vickroy@mhsil.com wrote: Awhile back we got an advertisement for a block alignment tool that would be helpful in recut cases. Can anyone share with me companies that have this device and approximate costs? James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] paraffin melting in VIP
I agree Rene! I also believe Sakura recommends not putting paraffin flakes directly in the containers. On Fri, May 11, 2012 at 2:27 PM, Rene J Buesa rjbu...@yahoo.com wrote: Regardless of the time it takes or of how many people do it, melting the paraffin directly in the VIP should not be done because it causes the heating elements to work extra reducing their useful life. They are quite expensive to replace!. Melt the paraffin outside the VIP and use the VIP only to keep the melted paraffin at the temperature you desire. René J. --- On Fri, 5/11/12, Gudrun Lang gu.l...@gmx.at wrote: From: Gudrun Lang gu.l...@gmx.at Subject: [Histonet] paraffin melting in VIP To: histonet@lists.utsouthwestern.edu Date: Friday, May 11, 2012, 1:58 PM HI! A question for those, who melt the paraffin directly in the VIP. How long does it take to melt the pellets in the VIP-oven? Thanks Gudrun ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Question about slides
Ward's Natural Science. On Mon, Mar 5, 2012 at 7:43 PM, Komal Gada kjg...@gmail.com wrote: Hello Histo-netters, I'm looking for a source which sells slides for a Tissue Identification class. Does anyone have any leads on where to go for something like this? Thanks, Komal ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Pro-Par
We do rotate our alcohols after eosin to minimize this problem. We recycle our alcohol from there so there is a cost savings. Every lab has their setup that works best for them while pleasing the pathologist(s) too. I love this forum because you can get informed opinions! On Tue, Feb 7, 2012 at 4:32 PM, Victor A. Tobias vtob...@uw.edu wrote: In a previous job we used Pro-Par and recycled it too for many years. I don't know if I would label it a drawback, but I felt the dehydrating steps after eosin required changing more often then if using xylene. There were days when we would get the dreaded bleeding eosin. Keep your reagents fresh and you shouldn't have any problems. We were hand coverslipping back then. Victor Victor Tobias HT(ASCP) Clinical Applications Analyst Harborview Medical Center Dept of Pathology Room NJB244 Seattle, WA 98104 vtob...@u.washington.edu 206-744-2735 206-744-8240 Fax = Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Thomas Jasper Sent: Tuesday, February 07, 2012 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pro-Par Hi Folks, Just curious...anyone using Pro-Par care to share an opinion? Have considered it off and on over the years and now wondering what folks think. Also, anyone using it with tape coverslippers? Maybe this has been asked and I wasn't keeping up with the thread. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pro-Par
We have been using ProPar for 15 yrs now. We were so glad to find something that allowed us to become virtually xylene free. We still need xylene for some things but not nearly the quantity that we used to keep around. The folks at Anatech were so helpful in helping us get started. We also recycle our ProPar which has saved us quite a bit of money over the years. I would recommend it! On Tue, Feb 7, 2012 at 1:29 PM, Thomas Jasper tjas...@copc.net wrote: Hi Folks, Just curious...anyone using Pro-Par care to share an opinion? Have considered it off and on over the years and now wondering what folks think. Also, anyone using it with tape coverslippers? Maybe this has been asked and I wasn't keeping up with the thread. Thanks, Tom Jasper Thomas Jasper HT (ASCP) BAS Histology Supervisor Central Oregon Regional Pathology Services Bend, Oregon 97701 541/693-2677 tjas...@copc.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 IMPORTANT NOTICE: This e-mail and any attachments may contain confidential or sensitive information which is, or may be, legally privileged or otherwise protected by law from further disclosure. It is intended only for the addressee. If you received this in error or from someone who was not authorized to send it to you, please do not distribute, copy or use it or any attachments. Please notify the sender immediately by reply e-mail and delete this from your system. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE
We use ProPar on our stainer. We rotate our 4 100% alcohols that are after eosin after every 3rd rack and we have stopped the leaching of eosin in our last ProPars. Not a big fan of exposing my staff to xylene unless I absolutely have to. Jen Campbell On Thu, Sep 15, 2011 at 9:44 AM, sdys...@mirnarx.com wrote: This happened to me several months ago. Our lab always has high humidity. If you are going to continue to use clear rite with humidity in the air, I would change it everyday. Also, change out your 100% because it's inevitably getting water in it too. I just switched my whole lab back to xylene...at least you can see the water in it =) Good Luck Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anuradha shrivastava Sent: Wednesday, September 14, 2011 9:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HE Hello everybody, Can anyone help me, In Leica autostainer we have after eosin 3 100% series, clear-rite/100% and then 3 clear rites. I observed that during humid days eosin was leaching . I replaced 2 clear rite after 50 :50 Abs/cler. with xylene. The slides were fine after wards. My question is , is it ok to keep the last clear rite or I should change that too to xylene. We had never used xylene before in the stainer. The coverslipper’s bath is filled with the clear rite.( xylene substitute), Please give your expert advice. Thanks . ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] harrasment for humble histotechs
I kicked ass on the HT practical! No way I was going to go through that hassle again. I am still puzzled as to why the powers that be got rid of it. It definitely tested how well you could follow directions too. On Sat, Aug 27, 2011 at 4:50 PM, histot...@imagesbyhopper.com histot...@imagesbyhopper.com wrote: :o). I'm not even sure I could find my results after all these years and 9 moves! I *do* remember how picture perfect those slides had to be though. Tiny air bubble=graded down. Folds=forget it. Knife marks=bad. I did both the HT and the HTL practicals and now new students don't even have to submit them. I didn't cheat, but over the years I saw students attempting to cheat. If I saw them, I would remind them that they were to work independently, but who knows what they did when I wasn't looking? Integrity is what you do when no one is looking. Sent from my iPhone On Aug 27, 2011, at 3:25 PM, mad...@verizon.net wrote: Gentle harrassment. I encourage you to look at you scores for your practical and written exam. Between gigs I am getting my important documents together and found my old scores for the HTL, no thte HT, however, both parts of the exam were taken by yours truly. Never got into the talk of practical vs no practical. I do think it si a good idea to keep it. Honest techs will be proud to do a practical. Looking at scores made me feel good that the next great job is right around the corner. Nick(Rocky) Madary, HT/HTL(ASCP)QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Slide Bright Question
We use ProPar and have found a way to survive the issue. Our stainer racks cause a lot of solution carryover so we rotate the alcohols after eosin after every third run. Once there is water in your final clearing containers they must be changed. Jen Campbell, HT(ASCP) On Fri, Jun 3, 2011 at 2:28 PM, sgoe...@mirnarx.com wrote: Ahhh!!! I had this same problem when I came to this lab too!! Plus if you put that xylene substitute crap (mine was clear rite) it screws up cell blocks that have been embedded into agar!! 5 experiments had to be repeated because of this stuff!! The eosin bleed is because you got water on the slides after the eosin. With xylene you can see the water pool at the bottom, but with these substitutes it just kind of becomes part of the solution. I have finally gone back to xylene! We had to buy hoods, but it's the cost of better histology =) Fixing the eosin bleed...uncoverslip, run through xylene (or let sit in the substitute for 15 or 20 minutes, then alcohols, then in running water for 10 minutes or so. Then change all your alcohols to fresh solutions (I would change the substitutes too), then from water to alcohol, eosin, etc. etc. and they should be fine. Good luck!! I wanted to pull my hair out a couple months ago because of that stuff...run away from it!!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christopher Conlisk Sent: Friday, June 03, 2011 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slide Bright Question Hello Everyone, I have worked in labs that use Xylene my entire career and I just started at a Lab That only uses a Xylene Substitute Slide-Bright. I am having problems with the HE. After staining and coverslipping (The slides look fine innitially), Then about 5-10 minutes after coverslipping the Eosin starts bleeding out all around the tissue. I have asked several of my Histotech Friends that are old timers and they say that Xylene Substitutes are awful at deparrifinization and awful at clearing. They told me that the alcohol isnt getting thoroughly cleared in the Slide Brite and then it is eventually leeching out after coverslipping??? Is this true and does anyone have any guidance for this issue? We also run MOHS slides on the same stainer and I keep all the reagents clean as a whistle. I really hate Xylene Substitute's Thanks C.S. Conlisk HT(ASCP), PBT(ASCP) Kansas City Skin and Cancer Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Recycled Xylene
Very true because if you notice the label on a purchased bottle of xylene it says Xylenes. Your recycled product should be pure xylene and thus a higher purity than what you started with. Our lab has been recycling since the mid '90's. We no longer process with xylene but we still have it in the lab for various things. Hope this helps. On Wed, May 25, 2011 at 11:11 AM, Rene J Buesa rjbu...@yahoo.com wrote: If you are using a good cracking recycling instrument the recycled xylene = 100% xylene and there cannot be any differences in behavior against pure-unused-mew xylene. That is what I always found for more than 15 years. René J. From: Marshall, Kimberly K kkmarsh...@anthc.org To: histonet@lists.utsouthwestern.edu Sent: Wednesday, May 25, 2011 9:20 AM Subject: [Histonet] Recycled Xylene Hello in Histo land. I know it is a subject brought up over and over again but I need to get the opinion of my fellow Histo techs on processing tissue with recycled Xylene. Yes I know it saves money and is better for the earth, but is the quality of the tissue the same??? Coverslipping and clearing slides with it I can see being ok, but processing with it??? It is not 100% after recycling. I could use any thought on the subject. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC run print outs
We had our in-house IT person create a print-out for us. We are a private lab so we are not inspected by CAP. That being said NYSDOH requires much of the same QC paperwork so we make do with what we have. On Wed, May 11, 2011 at 11:09 AM, WILLIAM DESALVO wdesalvo@hotmail.comwrote: To meet CAP requirements, we print un reports and send send to the pathologists with slides, they review, sign off and we keep for 2 yrs. All QC paperwork is kept for 2 yrs. William DeSalvo, B.S., HTL(ASCP) Date: Wed, 11 May 2011 10:01:03 -0500 From: amber.mcken...@gastrodocs.net To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC run print outs Does everyone print out IHC run reports after each run? If so, how long do you keep them and are the pathologists supposed to sign off on them with their slides? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell, HT(ASCP) Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] patient melanin pigment
We do a Melanin Bleach stain. On Wed, Apr 6, 2011 at 2:16 PM, Michele Carr michelecar...@yahoo.comwrote: Hi, I was wondering if there were a way to remove a patients melanin pigment on a slide. Any helpful advice would be appreciated. Thanks, Michele Carr Medical Laboratory Services ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory 61 Monroe Avenue, Ste B Pittsford NY 14534 P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] coverglass
I am looking for vendors for coverglass for an automated coverglasser. I want one where the coverglasses don't stick together. We are currently using Mercedes as our vendor. Any feedback out there for ThermoFisher? -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] job opening
Histonetters, Private dermpath lab in central/western NY is looking for a fully licensed (in NYS) histotech for a full time position. Please contact me directly by email or phone. -- Jen Campbell Supervisor of Technical Services Muhlbauer Dermatopathology Laboratory P: 585.586.5166 F: 585.586.3137 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help with Biocare polymer detection kit!!
I have been trying to get the Mach 1 polymer kit from Biocare to work on my K9 and feline tissue and I am getting terrible nonspecific staining in my positive and negative controls. It appears to be sticking to something in the glands of the intestinal epithelia. I am also getting nonspecific staining in my LN tissue. I decided to switch over to polymer detection kit because I had been dealing with the issue of endogenous biotin when using the streptavidin biotin-based detection system, and now I am getting this other nonspecific staining! I have tried doing runs eliminating my protein block and eliminating the negative control and primary antibody step altogether (to see if maybe the protein block or the diluent used in my negative control and antibody dilutions are causing the problem). I have also reduced the incubation time of both the probe and polymer from 15 min and 30 min, respectively, to 10 min each. I have tried cutting down my antigen retrieval time substantially as well. I'm not sure what else could be causing this. Any suggestions?? Thanks in advance, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Difference between Protease and Proteinase-K?
Hi All, I would like to try enzyme digestion on my CD31 (for K9 and feline tissue) which I haven't been able to get to work using citrate buffer pH 6.0 or pH 9.0. I have heard recommendations for both Protease and Proteinase-K but, do not know which one I should try out. Any feedback on the differences between the two? Thanks, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Has anyone used a biotin block in their antibody diluent?
Hi All, Has anyone ever used a Biotin block in their primary anitbody diluent? I have been having problems with nonspecific staining, which I suspect is due to endogenous biotin. I plan on decreasing my antigen retrieval time, as someone has told me that an antigen retrieval that is too vigorous may cause the unmasking of biotin, and its subsequent staining. I would like to know if anyone has had any luck using a biotin block in their diluent because I may try that as well. Thanks, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NY licensing
I'm looking into requirements for getting licensed as a histotech in NY and I am a little unsure of whether my educational experience will be considered a substantial equivalent to their requirements. They require education from a Histology program or something equivalent. I graduated from a 4 year university (UC Davis) with a B.S. in Animal Science (includes all science courses--biology, chem, organic chemistry, physics, anatomy etc) but I never specifically took a class on histology and its techniques used in the lab. Do you still think they will consider me eligible or would they require me to complete a histology program? I have yet to find the chance to call them since they close at 4:45 ET and I am on the west coast. Thanks in advance, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] recommendation for C-kit antibody
I'm looking for a C-kit antibody for IHC on FFPE K9 and Fel tissue. I've only found a couple of vendors that meet those requirements. Can anyone recommend one to me? Thanks, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Moving across the country...
Hi All, My husband and I will be moving from California to the east coast this late summer/early fall for his work.exciting but, a little scary! I have started browsing possible histotech jobs available in either NY, CT or NJ but, its a rather daunting task when you're not familiar with the area or the labs out there! And to add to the stress I am also currently studying for my HT certification which I will be taking in augustwhich I will hopefully pass so that I may have a little more luck job searching! Does anyone have any advice for me as to where I could look into or know of any possibilities out there? Thanks for any advice you may have. Jennifer Campbell VDx Veterinary Diagnostics Davis, CA phone: (530) 753-4285 fax: (530) 753-4286 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] nonspecific staining in liver
Hi All, I am having some problems with marked nonspecific staining in liver when running my CD3 and CD79a. I am using an avidin and biotin block, as well as a protein block. Any other suggestions as to what I could do to eliminate this? Thanks in advance, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fluoro Jade B (or C) protocol
Hi All, Does anyone have an IHC protocol for Fluoro Jade B (or C)? I have read it can be adapted from FITC to IHC but I can't find a protocol. Thanks, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] reagent alcohol in 50-55 gal drums
Hi All, Does anyone out there purchase their reagent alcohol in 50-55 gal drums? How much does this cost you, including the shipping handling and any other costs? We are trying to determine whether this would be a good idea or not. Thank you, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Thank you everyone!
Thank you for all of those who were so helpful in suggesting reading material my HT exam! I appreciate all of your input and kind words of encouragement. Wish me luck! Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] studying for ASCP certification exam
I am in the process of studying for my HT certification exam and was wondering if anyone had any recommendations on text books or study guides they found to be helpful. I am currently studying Histotechnology: A Self-Instructional Text, by Carson and just recieved the BOR study Guide for Histotechnology. Are there any other sources you would recommend? I have taken a look at the suggested reading list on the ASCP website but, there are quite a few books listed. Thanks in advance, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] special stains
I couldn't agree with you more, Liz! I am from a GLP lab as well and we have to undergo validation for every single new piece of equipment we acquire otherwise we are not considered GLP-compliant. Who is to say that your new stainer is going to stain slides in the same manner as your old stainer?--which is exactly why you have to validate your equipment and ensure that it is performing what the user has programmed it to perform and giving you accurate and consistent results. And we re-validate any time a change is made as well, whether we switch equipment or make a change to a staining protocol. We have special forms to document staining results and access to runtime logs on our stainer which allows us to ensure that the stainer carried out the correct protocol steps. Jen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] any info on Glutathione S-Transferase Pi (GST Pi)?
Hi All, Has anyone ever used this antibody in FFPE mouse or rat tissue? How easy of an antibody is it to work with? Other comments? We may be doing this for an upcoming project and I just wanted to know what I could be getting myself into. Thanks! Jen Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] leaving IHC slides in wash buffer
Hi Everyone, I have yet another IHC-related question. So after antigen retrieval, and then allowing my slides too cool, I place them in wash buffer before loading them on to the autostainer. Would leaving the slides in the wash buffer in the fridge for an extended period of time (say, 1 week) before continuing with immunostaining affect staining quality in any way? Thank you, Jen Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fite control slides
I am looking for a reputable vendor for Fite control slides. My pathologist would prefer they be leprosy containing skin slides. I also need the vendor to provide a positvely stained slide with the procedure as well. Thank you in advance Jen Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Granzyme B and F4/80 immunostaining
Hi All, I'm currently trying to work up Granzyme B and F4/80 on FFPE mouse tissue. I seemed to get a bit of non-specific staining for both and was wondering if there is any way I can eliminate some of this. For Granzyme B I am using a rabbit polyclonal from Abcam. My protocol goes like this: H202 block, citrate buffer HIER, Powerblock (casein-based, non-serum protein block), primary antibody, secondary antibody (rabbit-on rodent HRP polymer, from biocare), DAB, counterstain. My F4/80 is a rat anti-mouse primary from Serotec and the protocol is as follows: H202 block, citrate buffer HIER, Powerblock, avidin/biotin blocks, primary antibody, secondary antibody (Goat anti-rat, biotinylated, mouse-absorbed, from Biocare), HRP Label, DAB, counterstain. Is it true that if I switched to a serum block from the species in which the secondary was raised, instead of my non-serum protein block, it may help? Thank you in advance! Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rabbit-on-Rodent HRP polymer
Hi All, I recently purchased a rabbit-on-rodent HRP polymer from Biocare. I will be using this on mouse tissue, using a rabbit polyclonal CD3 as my primary. I just wanted to clarify a couple things about the protocol before I try it out tomorrow and was wondering if anyone could offer some assistance. First of all, I should aske if anyone has a protocol for this? What I really would like to make sure is that I will not have to use a label after the rabbit-on-rodent secondary ab. I believe that I will just have to apply my blocking agent, avidin/biotin blocks, primary antibody, secondary anitbody, followed by chromogen, right? My blocking agent is made up of casein and some other proprietary agents in a phosphate buffer (it is non-serum) so I was told that should work fine. Does this sound correct? Thanks in advance! I haven't been able to get through to tech support all day, so hopefully you will have some answers for me! Jen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD3 on mouse tissue
Hi Histonetters, I'm trying to work up CD3 on mouse tissue. I knew I would have to get a mouse-on-mouse kit since CD3 is a rabbit polyclonal but do you use some sort of rodent kit to eliminate background staining? My secondary antibody is part of a multilink kit from Biogenex and is capable of recognizing both mouse and rabbit primary antibodies. So, I'm afraid that it is recognizing endogenous antibodies in my mouse tissue. Any recommendations? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Is anyone familiar with Granzyme B antibody?
I'm trying to find out some information on Granzyme B antibody for immunostaining of FFPE mouse tissue. Does anyone have any vendors they could recommend and/or protocols? Thanks, Jennifer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] GSTP antibody
Hi All, Is anyone out there familiar with GSTP antibody? Do you have any information such as, pretreatment, incubation time, vendors etc? Thanks, Jen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] destaining cyto slides for immuno
Hi All, How would you go about destaining a cyto slide and running a CKAE1/AE3? I was told that acid alcohol to remove the stain beforehand may not be necessary as heat retrieval may remove it adequately enough. Does anyone have any recommendations? Thanks, Jen ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] decal solution
Does anyone know what the optimal pH for a nitric acid decal solution should be? Right now our's is reading below 1.0 when tested and we are having problems with our tissue being overdecalcified. Thank you in advance. Jennifer C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Question about DAB waste
We just purchased a Dako Autostainer and are in the process of figuring out how to get rid of the haz waste that we will be generating. The machine separates the haz from non-haz, thankfully. I've been communicating with our waste facility and have been having some difficulty. They want to know a lot of specifics like, how much waste we will be brining to them at a time and how often, what sort of containers we will be bringing it in etc. Do you let the haz waste carboy get to a certain level before emptying it? Is it ok to pour this waste into plastic containers of some sort (we were going to use empty distilled water bottles) to transport them to the facility? I would appreciate any insight. Thank you. Jennifer C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Toulidine blue stain
Hi everyone, I'm trying to find a protocol for a toulidine blue stain for paraffin sections. I've looked in different text books and online and can't seem to find anything helpful. Does anyone have a good protocol for this? I need to order the stain rather soon but, first I want to know how much I'm going to need to run the stain. Thanks in advance, Jen C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] pressure cookers for IHC
Hi everyone, Does anyone use a pressure cooker for heat induced epitope retrieval? I know there are a lot of fancy antigen retrieval units out there that you can buy specifically for IHC but, I know a lot of people just use a pressure cooker with the same success. Any specifics as to what type of pressure cooker to use? Protocol used as far as the temp and for how much time? Any info would be appreciated. Thanks, Jen C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Opinions on the Labvision 360 autostainer?
Hi everyone, Has anyone used the Labvision 360 autostainer? Any good or bad things about it? Is it suitable for IHC on animal tissues? Trying to sort out which stainer will work best for us and which is the most economical of course. I appreciate any feedback. Please no inquiries from sales reps. Thanks Jen C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] dako autostainers
We are looking into buying the Dako Autostainer Plus. I asked our rep if they still have any older models around to look into. He made it sound like there are older models but, the stainer itself has stayed the same, just the software has changed/improved. He said they don't even like selling the older models anymore b/c they are worried that the software would be out of date. I looked into this on Dako.com and found that there is the older Dado Autostainer. After reading through its description it looks like it just lacks a few of the bells and whistles (like a slide labeler for example) that the Autostainer Plus has but, otherwise looks like it gets the job done just the same. Does anyone have any feedback on either one of these machines and whether or not you think I should even bother looking into the older model? Thank you so much. Jen C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] In regards to a previous email I sent out..
Hi everyone, I sent out an email titled, Any Opinions on these microtomes? the other day asking for some opinions and feedback on a few microtomes I was interested in. I wanted to thank those of you who answered my email and gave me some helpful feedback. I have recieved a number of phone calls at work today from sales reps trying to sell me other models that I had not asked about in my email. I have already contacted our own sales rep and are therefore not looking for someone to sell us a new microtome but, rather someone to give us some feedback on the ones we are considering. If phone calls of this matter could be avoided, I would greatly appreciate it. Thank you, Jennifer Campbell ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Any opinions on these microtomes?
Hello everyone, Has anyone had any experience with the HM 340 E (Mikrom) or the Rotary Microtome RMTs 40, 50 or 60? We are looking to get a new microtome for paraffin sectioning but, would like one that is suitable for plastics in case we move that way in the future. These were recommended to me by a sales associate but, I would like to hear if anyone has some firsthand experience with any of these and what his or her thoughts are. Thanks a bunch, Jennifer Campbell Vdx Veterinary Diagnostics Davis, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
