Re: [Histonet] Alizaren Red and von kossa staining

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Dear Charles,

Can you provide more details about your needs? We might have gentler 
decalcifying solutions than the usual ones. Do you need histology services for 
your mouse skulls? We can handle a wide range of routine and special 
histological needs. Could you tell us where on the skull you plan to use 
Alizarin Red and Kossa Staining?

Best,
Michael



> Am 07.09.2023 um 22:34 schrieb Charles Riley via Histonet 
> :
> 
> Does anyone have any methods to soften bone without decalifier solutions to
> perform microtomy for these tests on E 18.5, P7, and 930 mouse skulls
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Re: [Histonet] Asking for software

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello all IPS/IPS users,

we used both printer, IPS and IPC just with the original printer driver and a 
database-application which offers us much more options than the application 
that comes with the printers (NiceLabel - as far as I know). So I think it 
should be possible to reinstall the printer driver from the CD ROM that comes 
with the printers and then to use the printers via Excel, Word, or any other 
software, best case from a database.

Kind regards
Michael


> Am 06.08.2022 um 21:43 schrieb Colleen Forster via Histonet 
> :
> 
> Donna,
> 
> I know first hand, if you have any computer issues that take out your
> software, ( power surge for example) Leica does NOT reinstall the program.
> You are left to find a different program. It took us a long time to get a
> program so we could use both the IPC/IPS again. The new software isn't even
> close to the program it came  with but it beats handwriting them.
> 
> This person may have had a similar problem so, like us, has the units and
> no software to run them. ☹️☹️
> 
> On Sat, Aug 6, 2022, 2:29 PM Willis, Donna G via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> Software comes with the unit when you purchase it from Leica.
>> 
>> Donna Willis
>> Anatomic Pathology Manager
>> Baylor University Medical Center
>> Baylor Scott Health
>> 214-820-2465 office
>> 
>> From: Carlos Torres Vega via Histonet 
>> Sent: Saturday, August 6, 2022 1:36:55 PM
>> To: histonet@lists.utsouthwestern.edu 
>> Subject: {EXTERNAL} [Histonet] Asking for software
>> 
>> 
>> CAUTION:  This email originated outside of BSWH. The actual sender is  (
>> histonet-boun...@lists.utsouthwestern.edu) which may be different from
>> the display address in the From: field. Avoid action unless you know the
>> content is safe. Report suspicious emails using the PhishAlarm button
>> located in your Outlook ribbon.
>> 
>> Terry Braud
>> You have used a Leica ISP  slide printer as stand alone unit, what
>> software did you use. ?
>> Because I have one but no software
>> 
>> 
>> Enviado desde mi iPhone
>> ___
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>> 
>> **
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Schumannstrasse 144
63069 Offenbach am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
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Re: [Histonet] human shoulder joint fixation

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello Merissa,

You can use a diamond band saw, as they are produced by EXAKT. This special 
technique will cut soft tissue quite  smooth without destroying the tissues. So 
you can cut slices with a few centimeter thickness which can be fixed in 
formaline after sectioning.

What kind of embedding and sectioning / staining you are planing? You can embed 
the tissues some special resins which are suitable for larger specimens.

If you need more information about the resin techniques and pathology saws, 
please contact me or Jerrod Roberts from EXAKT USA.

Kind regards
Michael



> Am 21.08.2020 um 17:37 schrieb M.O. via Histonet 
> :
> 
> Happy Friday, Histonetters!
> 
> I am doing some planning for a new project and wanted to get opinions on
> fixation of large pieces of tissue. We will have human shoulders, where we
> want to preserve the rotator cuff/joint. Cutting the tissue with a saw will
> damage the soft tissue, so we were thinking that post-fixation would be
> best for cutting slabs.
> 
> Does anyone have experience with fixing such large pieces of tissue? We
> typically use zinc buffered formalin for fixation. Would a vacuum work or a
> vacuum sealer?
> 
> Thank you for any input you might have
> Sincerely,
> Merissa
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
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Re: [Histonet] (no subject)

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Dear Kate,

very interesting question.

The both substances you mention are a bit difficult in this respect. In a 
general crystal structure phosphortungstic acid has 44 water molecules and 
phosphormolybdic acid 28 molecules of crystal water. This would mean, that for 
a an exact 5% solution you would need around 72,62 g of phosphortungstic acids 
and 72,73 of phosphormolybdic acid on 1.000 ml water. The standard for a 5 % 
solution of a solid substance without crystal water would be 52,63 g per 1.000 
ml.

However, in this case, the substances do not exactly have this number of water 
molecules in the crystal structure because they loose water and they are 
hygroscopic. This means that they take more water molecules from the air and 
that they partly melt in the bottle, if leave it open to long. 
 „• x H20“ - does not mean, that the manufacturers don’t want to give this 
information, its because it cannot be known exactly.

For these substances histological practice is to ignore the crystal water and 
to use the 52,63 g substance for 1.000 ml of water to get a 5% solution (or to 
add 5 g substance to 95 g water).

This does not have an crucial effect, because the mordanting process for which 
these acids are used has to be controlled by microscopy anyway. So if you want 
to make it faster you can add more substance. The important point is only, to 
make it always the same way.

Kind regards
Michael




> Am 24.01.2020 um 16:51 schrieb Kate Davoli via Histonet 
> :
> 
> I'm all set to DYI my own phosphomolybdic/phosphotungstic acid solution for
> running a Masson Trichrome, but I see that the former reagent was
> purchased as "phosphomolybdic acid hydrate".
> 
> All the recipes I have seen call for just "phosphomolybdic acid" but that
> is not a reagent that appears to exist without the water molecules coming
> along for the ride, unless you want to invest in chromatography grade
> stuff, which I think histology folks probably don't routinely do.
> 
> The recipes all call for equal gram amounts of each of these crystals, so
> here's my question:
> 
> Do I calculate how much weight the water is taking up and add more
> phosphomolybdic acid crystals (to account for its tagalong water molecules)
> than called for in the recipe?  Or are these recipes already assuming that
> phosphomolybdic acid HYDRATE is the reagent you have on hand, and I should
> stick with equal amounts?
> 
> This question is somewhat complicated by the fact that the molecular
> formula on the bottle is listed as H3Mo12O40P.XH2O ... which I think means
> the manufacturer won't bet on exactly how many water molecules are involved.
> 
> Any advice appreciated!
> 
> Katherine Davoli, BA, HTL(ASCP)CM
> Supervisor & Lab Manager, Tissue Culture & Histology Core Module
> Ophthalmic and Visual Sciences Research Center
> Department of Ophthalmology, University of Pittsburgh
> Mail Stop Code: EEI010901
> 930 Eye & Ear Institute, 203 Lothrop Street
> Pittsburgh, PA 15213
> (412) 647-8256davol...@upmc.edu and kdav...@gmail.com
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60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
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Re: [Histonet] Need a Slide Scanner

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Dear Kimberly,

we have two systems here in our lab, both with microscopes from ZEISS. The 
first one is a TissueFaxs-System which is for scanning of maximum 8 Slides and 
special optical requirements (polarization, phase contrast, etc.). This system 
is quite good and flexible and you can use almost all optical parameters of the 
microscope, including fluorescense. However, compared to slide scanners its 
relatively slow. Our system is about 5 or 6 years old, but I know that 
TissueGnostics developed larger and faster systems working with a complete 
microscope and a loading system for 100 or 200 slides. If you have larger 
slides, TissueGnostics also provides slide holder for very larger slides (as 
far as I remember 15 x 24 cm).

Our second system for larger amounts is the ZEISS Axioscan 
(https://www.morphisto.de/en/service/digital-pathology/), which is in our 
opinion the best scanning system on the market, since it also works with a 
complete microscope and a very good Software (ZEN). ZEN looks a bit difficult 
at the first sight, but it offers many many features to get very very good 
scanning results. 
For scanning of slides speed is not the most important parameter, but correct 
optical representation. Many scanners are just fast, because they close the 
aperture diaphragm and light-field diaphragm, to generate high contrast, but as 
everybody knows who is familiar with working on a manual microscope, your 
produce virtual (fake) structures.

With the ZEISS Axioscan you can make Z-stacks, which means that the same 
slide/region will be scanned several times at different focus layers, so that 
you can finally go through this stack on the computer. The ZEISS Axioscan 
starts with a focus map, to save focussing time, and its possible to optimize 
this focus map for speed or for quality.
ZEISS also offers a free ZEN-light version, which can be used on every computer 
to open and analyse the scanned slides.

The ZEISS Axioscan is also able to handle larger slides (50 x 76 mm) and it can 
be upgraded for fluorescence and polarisation.

If you like to see some sample, please contact me, I think we can also make a 
contact to ZEISS, if you are interested.

Kind regards
Michael

 



> Am 09.01.2020 um 19:39 schrieb Glover, Kimberly via Histonet 
> :
> 
> Hi,
> 
> I am interested in purchasing a slide scanner for my lab so we will have the 
> ability to share slides between sites. Can someone suggest a good scanner, 
> make/model?
> 
> Kimberly Glover
> Product Manager, Histology and Hematology
> Warrington, PA
> 
> 
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

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Re: [Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background)

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

The sirus-red stain is a stain to differentiate collagene I against collagene 
III in polarized llight, so in bright field all fibres will be red, just if you 
change to polarised light, you can differentiate both kinds of fibres by their 
birefrigence which is orange to yellow for collagene 1 and green for the 
collagene 3.

Cheers
Michael


> Am 10.08.2019 um 06:29 schrieb John Kiernan via Histonet 
> :
> 
> I've never seen the kind of staining you describe, abi jag, with "the 
> complete section become stained as red" but I've never used the method on 
> sections of liver.
> 
> You should get red collagen and yellow hepatocytes with blue nuclei. The 
> strongly acidic picrosirius stain, applied for an hour, always greatly 
> weakens (differentiates) a prior nuclear stain with Weigert's or Lillie's 
> iron-haematoxylin.  It's also quite easy to lose some or all the yellow in 
> the washing and dehydration of the stained sections. Most users of this 
> method are interested only in collagen fibres and do not mind if the nuclear 
> and cytoplasmic colours get lost. The iron-haematoxylin nuclear stain is 
> often omitted.
> 
> 
> It is necessary to have the right dye. It must be sirius red F3B (= CI 35780 
> = Direct red 80). This is still used as a textile dye, with several suppliers 
> and trade-names. See 
> http://www.worlddyevariety.com/direct-dyes/direct-red-80.html#respond
> 
> Direct Red 80 - 
> worlddyevariety.com
> www.worlddyevariety.com
> List of Suppliers: Direct Red F3,Direct Fast Red BA,Direct Fast Red F3B. 
> Tianjin Yadong Group . ACDI Red 8 BLN(Aakash Chemicals & Dyestuffs 
> Inc)Alacodirect Red 2BL(Classic Dyestuffs Inc)AmbidirectRed 3BL( Thai Ambica 
> Chemicals Co Ltd) Anadurm Red D-BA( Albion Colours Ltd) Arid Red 8 BLN ( 
> Aashiana Dyestuffs Inc) Best Direct Supra Red F3B( Oriental Giant Dyes and 
> Chemical Ind Corp)
> Other dyes with "sirius red" in their trivial names probably are not suitable 
> if they are not the product recognized in the Colour Index as  CI 35780,  
> Direct red 80. An example of a different dye is sirius red 4B (= CI 28160 = 
> Direct red 81), which has been prescribed for use in some staining techniques 
> as a dye with properties similar to those of eosin Y and acid fuchsine.
> 
> 
> The Biological Stain Commission has standards for sirius red F3B as a 
> collagen stain. Dye from a batch that meets their standards will be OK for 
> your method.
> 
> 
> My Histonet post from which you are using this method must date from the 
> 1990s, when I knew that picro-sirius red solutions were good for 5-6 years. 
> (I would have written 3 years to be cautious.)  With more experience with 
> stored and newly made solutions, I feel confident in saying they keep for 
> more than 20 years.
> 
> 
> It might get contaminated from too much iron-haematoxylin extracted from 
> previously stained  slides. I don't know what this would do.
> 
> 
> The most obvious cause of red cytoplasmic staining by picrosirus is not 
> enough picric acid (yellow powder in the bottom of the bottle) in the 
> staining solution.
> 
> 
> It's unfortunate that items found with HistoSearch are undated. It doesn't 
> matter in this case, but many Histonet items become outdated after only a 
> year or two; antibodies and automated staining are examples of fields in 
> which you need to know the age.
> 
> Keep in touch about your sirius red problem.
> 
> John Kiernan
> John A. Kiernan MB, ChB, PhD, DSc
> Professor Emeritus, Anatomy & Cell Biology
> University of Western Ontario, London, Canada
>https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html
> Also  Secretary,  Biological Stain Commission, Inc.
>https://biologicalstaincommission.org
> = = =
> 
> From: abi jag via Histonet 
> Sent: 09 August 2019 11:29
> To: histonet@lists.utsouthwestern.edu 
> Subject: [Histonet] Recent issues with picro sirius red staining (entire 
> liver section become red, no yellow background)
> 
> Hello Histonetters,I am writing this to seek your help regarding a very 
> recent problem that I am currently facing with Picro Sirius red staining of 
> lab animal (mouse and rat) liver samples. I follow the procedure that was 
> provided by John Kiernan in the histonet archives (please see below), which 
> was working very well. Quite recently, the complete section become stained as 
> red. Usually, collagen in the sections get stained as red with a yellow back 
> ground. Please note that there was no change in the procedure/reagents etc, 
> It will be of great help if you help me in troubleshooting this issue.With my 
> best regards,Abijag
> Sirius red collagen procedure
> 
> |
> |
> |  |
> Sirius red collagen procedure
> 
> 
> |
> 
> |
> 
> |
> 
> 
> 
> 
> Solution A. Picro-sirius red
> 
>  Sirius red F3B (C.I. 35782): 0.5 g
>  Saturated aqueous solution
>of picric acid:500 

Re: [Histonet] Victoria blue for lung tissue

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

There is also a Kit and the ready to use solution available from MORPHISTO 
Germany.

Just contact the sales office: i...@morphisto.de

Cheers
Michael



> Am 27.07.2019 um 23:53 schrieb Tony Henwood (SCHN) via Histonet 
> :
> 
> Methods found here:
> 
> https://www.ihcworld.com/_protocols/special_stains/miller's_elastic_ellis.htm
> 
> http://www.histosearch.com/histonet/Dec98/Millersstainforelasticfib.html
> 
> https://www.sakuraus.com/getattachment/774d89be-4b32-4d69-bb02-8dea75c52c0c/858
> 
> ?
> 
> 
> 
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Principal Scientist, the Children's Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
> 
> From: Bob Richmond 
> Sent: Saturday, July 27, 2019 11:27:54 AM
> To: Tony Henwood (SCHN)
> Subject: Re: [Histonet] Victoria blue for lung tissue
> 
> Is this method published anywhere that "Amy" would likely have access to? 
> This looked to me like one of those cases of the blind leading the blind that 
> are all to common in research involving histochemistry.
> 
> On Fri, Jul 26, 2019 at 8:44 PM Tony Henwood (SCHN) 
> mailto:tony.henw...@health.nsw.gov.au>> wrote:
> Victoria Blue is the dye used in Miller's Stain for Elastic Tissue.
> It is also used in the Roche Ventana Benchmark Stainer to stain elastic 
> Tissue:
> 
> Miller PJ (1971) An elastin stain. Med Lab Technol 28, 148-149
> 
> Karen Percival & Zaher Radi (2017) Comparison of five elastin histochemical 
> stains to identify pulmonary small vasculature, Journal of Histotechnology, 
> 40:3, 73-78
> 
> Yufeng Yu & Clifford M. Chapman (2000) "Elastic Tissue Staining in Human 
> Skin" Histologic 32(1): 12
> 
> Roten SV, Bhat S, Bhawan J. Elastic fibers in scar tissue. J Cutan Pathol 
> 1996: 23: 37-42.
> 
> 
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Principal Scientist, the Children's Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
> 
> 
> From: Bob Richmond via Histonet 
> mailto:histonet@lists.utsouthwestern.edu>>
> Sent: Saturday, July 27, 2019 4:10 AM
> To: 
> Histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Victoria blue for lung tissue
> 
> Amy (where?) asks: >>I was asked to do Victoria Blue stain on rodent FFPE
> lung tissue to exam thickness of artery. Could anybody recommend a good
> vendor of this reagent kit?<<
> 
> You can get Victoria Blue R (Colour Index 44040) from Sigma-Aldrich and
> several others. I couldn't find anyone who offers a kit, only the dry dye.
> There are other dyes called Victoria Blue, reported to give the same
> results.
> 
> You'd have to find a method for preparing it as an elastic stain, and I
> couldn't find such a method either with Google or in my old books. The
> requester needs to supply you with a method. I suspect the requester is
> reading an old article. There are stains for elastic tissue (which is I
> suppose what you want to "exam thickness of artery") that are a lot easier
> to get.
> 
> Bob Richmond
> Samurai Pathologist
> Maryville TN
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MORPHISTO GmbH
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Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 

Re: [Histonet] Elephant Tissues

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello Jennifer,

I do not have experiences with elephant tissues, but with other tissues that 
have thick connective fibres. Sodium hydroxide may help, however, I would 
recommend resin embedding, too. For this purpose Technovit 7100 would be a good 
and easy to use resin. Its easy to infiltrate and can be cut with rotary 
microtomes with some special knifes, and there are many stains that work on 
Technovit 7100 embedded specimens. Please let me know, if you need further 
information. We have a local dealer in US.

Kind regards
Michael

> Am 06.09.2018 um 16:41 schrieb Jennifer Phinney via Histonet 
> :
> 
> Hi Paula,
> One of my pathologists thinks it could be the collagen in the tissues making 
> them difficult to cut.  We have tried nair, fabric softener, and even 
> decaling the tissues and nothing helps.  The tissues shred immediately when 
> trying to section making it impossible in some cases to actually get a slide. 
> When I melted a particularly difficult block down to separate out the 
> different tissues, it turned out to be the lymph node that was the worst.
> 
> We are going to try some of the cream you use to soften scar tissues to see 
> if that has any effect.
> 
> As a veterinary histology lab we routinely work on a variety of different 
> species, and so far the elephant tissue is the only one giving us problems.
> 
> Thanks for everyone’s help,
> Jennifer
> 
> From: P Sicurello 
> Sent: Wednesday, September 5, 2018 4:46 PM
> To: Jennifer Phinney 
> Cc: HistoNet 
> Subject: Re: [Histonet] Elephant Tissues
> 
> Jennifer,
> 
> I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale, 
> giraffe, and even human mummy tissues.  With the exception of the mummy 
> tissue being a bit dry, they all embedded and cut like human tissue.
> 
> What is it that is making them hard to cut?
> 
> Sincerely,
> 
> Paula Sicurello, HTL (ASCP)CM
> 
> Histotechnology Specialist
> 
> UC San Diego Health
> 
> 200 Arbor Drive
> 
> San Diego, CA 92103
> 
> (P): 619-543-2872
> 
> 
> 
> Confidentiality Notice: The information transmitted in this e-mail is 
> intended only for the person or entity to which it is addressed and may 
> contain confidential and/or privileged material.  Any review, retransmission, 
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> prohibited.  If you received this e-mail in error, please contact the sender 
> and delete the material from any computer.
> 
> 
> On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet 
> mailto:histonet@lists.utsouthwestern.edu>> 
> wrote:
> Hello Histonetters,
> Does anyone have experience processing and cutting elephant tissues?  Any 
> tips, tricks, or advice?  My lab has had some elephant cases recently and the 
> tissues are unexpectedly (to us) difficult to cut.
> 
> Thanks for any help,
> Jennifer Phinney QIHC
> Kansas State University
> Veterinary Diagnostic Lab
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PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
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[Histonet] Specimen cooling during sectioning

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello Histonetters,

since the summer in Germany is recently quite warm I want to ask about your 
experiences with specimen cooling during and before sectioning via microtome? 
Does anyone use Leica CoolClamp or similar cooling units? What do you think 
about this? Our experience is, that cooling is quite good for sectioning, but 
the recent tools like CoolClamp or CoolCut do not perform significant cooling, 
so we tried to construct something more efficient. Now our question is, if this 
would be of broader interest?

Kind regards
Michael
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Re: [Histonet] New Bone Stain

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello Jessica,
Hello Natalia,

we have discussed the question of bone staining methods in our lab and we would 
recommend the following stainings, which will work on resin embedded material:

(1) For an overview and differentiation of various types of bone the 
traditional MASSON-GOLDNER or AZAN staining is quite fine, and should be the 
first try because these stains are more or less easy and fast and the staining 
solutions are not too expensive.

(2) MOVAT-Pentachrome in the original and in the modification after VERHOEFF 
also gives quite good differentiations of resin embedded bones and shows many 
details even in different types of bone. We use these staining here in many 
research projects we do for pharma companies that develop bone replacement 
materials.

(3) van KOSSA is also a very good silver impregnation to show mineralized bone, 
AND there is a possible combination with the MOVAT Pentachrom-Staining.

(4) Alizarinred can also be used to show different gradients of mineralisation; 
the staining kit contains solutions with several pH-values.

(5) Picro-Siriusred and other combinations of picric acid and other stains 
(picro fuchsin, picro polychrom, picro indigocarmin, and 
orcein-picroindicocramin can show good differentiations on tissues and 
cartilage and bone structures.
Remark: Sometimes its a bit difficult to establish picric acid based stainings 
on resin embedded tissues, if the resin has not been completely removed (which 
is possible with technovit 9100 (and some other MMA’s), but not with technovit 
7200).

(6) LEVAI-LACZKO's stain provides a good differentiation between mineralized 
bone matrix, Osteoid and synthetic bone substitutes.

(7) A special „Bone Stain“, published by Villanueva in 2009 
(https://www.tandfonline.com/doi/abs/10.3109/10520297409116928 
) works best on 
fresh material. We have not yet tested it completely on resin embedded tissues, 
but some of our customers used it successfully.
For this stain, a „staining powder" has to be synthesized from various single 
stains (basic fuchsin, orange G, fastgreen and azure II) and finally mixed into 
a ready to use staining solution; we produce the stain powder and ready to use 
solution here in our lab.

(8) Last but not least HEROVICI’s stain might help beacuse it differentiates 
between new and old collagen structures. Though it was originally developed for 
diagnostics in wound repair, some of our customers use it to differentiate 
between mineralized bone, new bone (Osteoid) and synthetic bone substitutes.

For all staining procedures on resin embedded material its necessary to edge 
the surface with citric acid or hydrogen peroxide, depending on the kind of 
resin you are using. If it is technovit 7200 citric acid should work fine, for 
technovit 9100 you can also try to remove the resin with methoxyethylacetate 
and acetone. Please note, that there can be a significant difference in the 
quality of the stain between Technovit 9100 and Technovit 7200, depending on 
what you want to see. In general, the stain on Technovit 9100 sections will be 
more powerful and brilliant than that on Technovit 7200 sections. 

As mentioned in my previous mail, we have a local dealer in Oklahoma, and if 
you or somebody else is interested in our ready-to-use stainings kits, just let 
me know, I will then make the direct contact.

Kind regards
Michael



> Am 10.07.2018 um 22:15 schrieb Jessica Riggleman via Histonet 
> :
> 
> Hey Jennifer,
> Thanks for your help. I have two questions:
> 
> 1. What is the point of decalcifying if they are in plastic?
> 2. Did you see the counterstain? Right now only the bone is staining. How 
> long did you leave these in the counterstain for?
> Thanks!
> Jessica
> 
> From: Mac Donald, Jennifer [mailto:jmacdon...@mtsac.edu]
> Sent: Tuesday, July 10, 2018 4:07 PM
> To: Jessica Riggleman ; 
> histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] New Bone Stain
> 
> I used to do it on plastic sections of in-de-calcified bone biopsies. It’s a 
> silver reaction.
> 
> Get Outlook for iOS
> 
> 
> 
> _
> 
> Jessica Riggleman | Research Associate
> 
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> 
> Confidentiality Note:  This email is confidential and intended solely for the 
> use of the individual to whom it is addressed. If you are not the intended 
> recipient, be advised that you have received this email in error and that any 
> use, dissemination, forwarding, printing, or copying of this email is 
> strictly prohibited. If you have received this email in error please contact 
> the sender. Any views or opinions presented are solely those of the author 
> and do not necessarily represent those of Globus Medical, Inc. Although this 
> email and any attachments are believed 

Re: [Histonet] New Bone Stain

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello Jessica,

we have a special „bone stain“ staining solution in our portfolio which is 
exactly for the purpose you described. Osteoid will be stained green or red to 
dark red, incomplete mineralized bone bone light red or orange yellow and the 
demarcation zone light green. Its also possible to differentiate lacunae, 
canaliculae, feathered bone, etc. This special stain was developed for 
diagnosis of bone diseases and I think it should be suitable for your purpose.

If you are interested in this staining kit, please let me know, we have a local 
dealer in Oklahoma and so it would be easy to deliver the ready-to-use staining 
solution to you.

Kind regards
Michael



> Am 09.07.2018 um 22:16 schrieb Jessica Riggleman via Histonet 
> :
> 
> Hello,
> I am trying to assess new bone vs. old bone in an animal model. Any 
> suggestions on thick section stains? I have heard Van Gieson's?
> 
> Thank You,
> Jessica
> 
> 
> _
> 
> Jessica Riggleman | Research Associate
> 
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> 
> Confidentiality Note:  This email is confidential and intended solely for the 
> use of the individual to whom it is addressed. If you are not the intended 
> recipient, be advised that you have received this email in error and that any 
> use, dissemination, forwarding, printing, or copying of this email is 
> strictly prohibited. If you have received this email in error please contact 
> the sender. Any views or opinions presented are solely those of the author 
> and do not necessarily represent those of Globus Medical, Inc. Although this 
> email and any attachments are believed to be free of any virus or other 
> defects which might affect any computer or IT system into which they are 
> received, no responsibility is accepted by Globus Medical, Inc. for any loss 
> or damage arising in any way from the receipt or use thereof.
> 
> 
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

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Re: [Histonet] MMA or Epoxy Embedding

[Michael Gudo (Morphisto GmbH) via Histonet]

Dear Jessica,

the best way to embed screws or any other implant material or bones or teeth or 
any compounds is to Technovit 7200. It a single component Acrylate that hardens 
in blue and white light within a few hours.

We are the exclusive distributor for all Histology Technovits from Kulzer, so 
please contact me personally for further details regarding price, shipping and 
detailed instructions. We can ship worldwide and have some 

Kind regards
Michael

P.S. Here is a short description:
https://www.morphisto.de/en/resin-histology/technovit-resins/technovit-7200/



> Am 21.06.2018 um 20:59 schrieb Jessica Riggleman via Histonet 
> :
> 
> Hi Everyone,
> I am trying to embed screws in plastic (in the hopes to see breakage in the 
> screw, etc). Right now I use specifically a mixture of methyl methacrylate, 
> poly methyl methacrylate, and benzoyl peroxide. However this usually takes a 
> few weeks to embed/dry. I am looking for something a little faster (perhaps a 
> week max?).
> 
> Thank You,
> Jessica
> 
> 
> _
> 
> Jessica Riggleman | Research Associate
> 
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> 
> Confidentiality Note:  This email is confidential and intended solely for the 
> use of the individual to whom it is addressed. If you are not the intended 
> recipient, be advised that you have received this email in error and that any 
> use, dissemination, forwarding, printing, or copying of this email is 
> strictly prohibited. If you have received this email in error please contact 
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> and do not necessarily represent those of Globus Medical, Inc. Although this 
> email and any attachments are believed to be free of any virus or other 
> defects which might affect any computer or IT system into which they are 
> received, no responsibility is accepted by Globus Medical, Inc. for any loss 
> or damage arising in any way from the receipt or use thereof.
> 
> 
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60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
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Re: [Histonet] Goldner's Trichrome Thick Sections

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Dear Jessica,

what kind of MMA have you used? If it is Technovit 9100 we have an established 
procedure here in our lab. How many slides do you have? We can make you an 
offer to stain them here in our lab for you. There are several trichrome or 
polychrome stains possible. 

Best regards
Michael


> Am 08.06.2018 um 20:04 schrieb Jessica Riggleman via Histonet 
> :
> 
> Hello,
> Looking for a protocol for staining thick plastic methyl methacrylate 
> sections (100-150 um). Goldner’s or any trichrome stain would work.
> 
> Thank You!
> Jessica
> 
> 
> 
> _
> 
> Jessica Riggleman | Research Associate
> 
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> 
> Confidentiality Note:  This email is confidential and intended solely for the 
> use of the individual to whom it is addressed. If you are not the intended 
> recipient, be advised that you have received this email in error and that any 
> use, dissemination, forwarding, printing, or copying of this email is 
> strictly prohibited. If you have received this email in error please contact 
> the sender. Any views or opinions presented are solely those of the author 
> and do not necessarily represent those of Globus Medical, Inc. Although this 
> email and any attachments are believed to be free of any virus or other 
> defects which might affect any computer or IT system into which they are 
> received, no responsibility is accepted by Globus Medical, Inc. for any loss 
> or damage arising in any way from the receipt or use thereof.
> 
> 
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PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
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Re: [Histonet] Masson's Trichrome Troubleshooting

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Dear Tasha,

well, I think you should modify your protocol:

(1) don’t use microwave
(2) wash in ethanol after Bouin and not in water (70 - 80 %)
(3) tap water as long as Weigert working solution
(4) Bieberich Scarlet is not the correct stain for Masson, you should use Acid 
fuchsine + Ponceau 2R + Azophloxine
(5) use a higher concentration of phosphortungstic acid, or / and least leave 
the sections much longer, up to 30 minutes in this step
(6) do not „air dry“, but remove water with ethanol and ethanol with xylene, 
then you should get very nice results.

Well, and finally, if you allow I would like to offer you the Masson Kit / 
Masson Goldner Kit our company offers for quite brilliant stainings: 

https://webshop.morphisto.de/catalogsearch/result/?q=Kit+MASSON+trichrom 


Please do not hesitate to contact us, if you have any further questions.

With best regards
Michael


> Am 30.05.2018 um 14:15 schrieb Campbell, Tasha M. via Histonet 
> :
> 
> Hello all,
> 
> I am having issues with my trichrome stain and I am about to lose my mind!  
> We just started doing it in house (although at my previous job I had done 
> trichrome by hand for years so I am not a stranger to it. And I never had 
> issues with it).  When I brought it in house at my current lab, I ordered the 
> same kit that I was familiar with.  Its PolySci.  I did the stain about 5 or 
> 6 times and then all the sudden it quit working.  There was red where it 
> should be blue.  And there was blue staining but it was all in the crypts.   
> I tried tweaking the stain a few times and nothing worked so I got a new kit. 
>  The first 2 times I used the new kit, it worked perfect!  But after that it 
> is back to doing the same thing again!  The collagen is not staining blue.  
> It is staining red.  Can anyone please tell me why this is happening?  I 
> never had this issue before!  Thanks in advance!! See my protocol below.
> 
> 1. Mordant in Bouin's solution, microwave 5 minutes, allow to stand 15 
> minutes.
> 2. Wash in running tap water to remove the picric acid, 5 minutes.
> 3. Weigert's Working Hematoxylin, 10 minutes.
> 4. Blue in running tap water for 5 minutes, rinse in distilled water.
> 5. Biebrich scarlet for 5 minutes.
> 6. Rinse in distilled water.
> 7. Phosphotungstic/phosphomolybdic acid for 10 minutes.
> 8. Transfer directly into Aniline blue for 5 minutes.
> 9. Rinse in distilled water.
> 10. 1% Acetic acid for 1 minute.
> 11. Quick rinse and air dry.
> 12. Coverslip
> 
> 
> 
> 
> Tasha Campbell, B.S.,HTL(ASCP)
> Frederick Gastroenterology Associates
> 310 W. 9th St.
> Frederick, MD 21701
> 301-695-6800 ext. 144
> 
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder 
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Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit 
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Re: [Histonet] Umbilical Cord Embedding

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Dear Jessica,

fixation should be at least 48 hours, however it is not problematic if the 
tissues stay longer in these solutions. Important point is, to remove the 
picric acid, which is a component of the Bouin solution, with several steps of 
70 - 80 % of ethanol, so after fixation go directly into 70 - 80 % ethanol, and 
change the ethanol several times, until the ethanols stay clear. To improve 
this process, you can add a few drops of ammoniak.

Best regards
Michael

 

> Am 24.05.2018 um 23:49 schrieb Jessica Riggleman 
> :
> 
> Michael,
> Thank you so much. How long do you live it in the fixation 
> solution/alcohol/etc for typically?
>  
> Thank You,
> Jessica
>  
>   Jessica Riggleman | 
> Research Associate
> 
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax: 
> An ISO 13485 Registered Company
>  
> website  | map 
> 
>  | email  
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> Confidentiality Note:  This email is confidential and intended solely for the 
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> From: Michael Gudo (Morphisto GmbH) [mailto:michael.g...@morphisto.de 
> ] 
> Sent: Thursday, May 24, 2018 3:59 PM
> To: Jessica Riggleman  >
> Cc: histonet@lists.utsouthwestern.edu 
> 
> Subject: Re: [Histonet] Umbilical Cord Embedding
>  
> Dear Jessica,
>  
> we have done embedding in paraffine and the results are quite good. The 
> critical point is fixation. Due to the high proportion of water in the 
> tissue, the best fixation is possible with Bouin-solution or - if you want to 
> do IHC stainings, Zamboni-fixation (which is a variant of Bouin with a 
> buffered pH value).
>  
> With trichromatic or polychromatic stainings you will get very colorful 
> results.
>  
> Best regards
> Michael
>  
> 
> 
> Am 24.05.2018 um 21:01 schrieb Jessica Riggleman via Histonet 
>  >:
>  
> Hello,
> Does anyone have any experience embedding umbilical cord tissue? Preferably 
> paraffin but we may try plastic as well. Any help/protocols/etc. would be 
> appreciated.
> 
> Thank You,
> Jessica
> 
> 
> _
> 
> Jessica Riggleman | Research Associate
> 
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> 
> Confidentiality Note:  This email is confidential and intended solely for the 
> use of the individual to whom it is addressed. If you are not the intended 
> recipient, be advised that you have received this email in error and that any 
> use, dissemination, forwarding, printing, or copying of this email is 
> strictly prohibited. If you have received this email in error please contact 
> the sender. Any views or opinions presented are solely those of the author 
> and do not necessarily represent those of Globus Medical, Inc. Although this 
> email and any attachments are believed to be free of any virus or other 
> defects which might affect any computer or IT system into which they are 
> received, no responsibility is accepted by Globus Medical, Inc. for any loss 
> or damage arising in any way from the receipt or use thereof.
> 
> 
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> 
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> Weismüllerstr. 45
> 60314 Frankfurt am Main
> Telefon:   069 / 400 3019 - 62
> 

Re: [Histonet] Umbilical Cord Embedding

[Michael Gudo (Morphisto GmbH) via Histonet]

Dear Jessica,

we have done embedding in paraffine and the results are quite good. The 
critical point is fixation. Due to the high proportion of water in the tissue, 
the best fixation is possible with Bouin-solution or - if you want to do IHC 
stainings, Zamboni-fixation (which is a variant of Bouin with a buffered pH 
value).

With trichromatic or polychromatic stainings you will get very colorful results.

Best regards
Michael


> Am 24.05.2018 um 21:01 schrieb Jessica Riggleman via Histonet 
> :
> 
> Hello,
> Does anyone have any experience embedding umbilical cord tissue? Preferably 
> paraffin but we may try plastic as well. Any help/protocols/etc. would be 
> appreciated.
> 
> Thank You,
> Jessica
> 
> 
> _
> 
> Jessica Riggleman | Research Associate
> 
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
> 
> Confidentiality Note:  This email is confidential and intended solely for the 
> use of the individual to whom it is addressed. If you are not the intended 
> recipient, be advised that you have received this email in error and that any 
> use, dissemination, forwarding, printing, or copying of this email is 
> strictly prohibited. If you have received this email in error please contact 
> the sender. Any views or opinions presented are solely those of the author 
> and do not necessarily represent those of Globus Medical, Inc. Although this 
> email and any attachments are believed to be free of any virus or other 
> defects which might affect any computer or IT system into which they are 
> received, no responsibility is accepted by Globus Medical, Inc. for any loss 
> or damage arising in any way from the receipt or use thereof.
> 
> 
> ___
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PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

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Re: [Histonet] Ethanol in tissue processing

[Michael Gudo (Morphisto GmbH) via Histonet]

Well, in Germany, and as far as I know in Europe, most labs use ethanol at 96 
and 99 percent as the last two steps in tissue processing. These ethanols are 
are denatured with MEK (= Butanon, Methylethylketon). Methanol or isopropyl is 
not allowed as denaturation agent in Europe, otherwise you have to pay the full 
alcohol tax.

In our lab we we use 96 % ethanol and as last step and then,  before going to 
xylene we use isopropanol which has a better dehydration effect than ethanol. 
99 % ethanol takes water from the air and quite fast becomes a 96 or 95 % 
ethanol and you will have some water left in the specimens when you to to 
xylene.
So our experience are that the best quality of dehydration can be reached with 
ethanol as high as possible and finally isopropanol before the xylene (2 
steps). 

With best regards
Michael


> Am 12.05.2018 um 22:48 schrieb Jay Lundgren via Histonet 
> :
> 
> Most places use "reagent alcohol" which, from most suppliers, is a mongrel
> blend of whatever is cheapest, and not even listed on the label. It's
> usually made up of a blend of ethanol, methanol, and isopropyl.  Good luck
> finding what the percentages are.  I've called one vendor before, and even
> they couldn't tell me.
> 
> 
> Virus-free.
> www.avg.com
> 
> <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>
> 
> On Sat, May 12, 2018 at 2:48 PM, Mac Donald, Jennifer via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> We use reagent alcohol on the processor and for staining.
>> 
>> Get Outlook for iOS
>> 
>> From: Jamie Watson via Histonet 
>> Sent: Saturday, May 12, 2018 9:06:01 AM
>> To: Histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] Ethanol in tissue processing
>> 
>> Hi All,
>> 
>> Does anyone know what percentage of labs use ethanol vs. denatured alcohol
>> or isopropyl alcohol on the tissue processor?
>> 
>> Jamie
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
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Re: [Histonet] Pineapple sponge sections

[Michael Gudo (Morphisto GmbH) via Histonet]

Hello Nancy,

we have done some histology on sponges and tried paraffin and resin embedding. 
Due to the spicules and also biofilms and sand etc. the best results could be 
reached with resin embedding in technovit 7100 or better 9100 or 7200 with 
cutting grinding techniques. Sectioning of 9100 or 7100 is possible, but the 
spicules can cause damages in the sections, so best results can be reached with 
cutting grinding.

There are several trichrome and PAS stainings which give nice results, but we 
have learned that individual testings have to be done, because the sponge 
tissues are very different in their preservation and stainings abilities.

For fixation you should use formaline, but other fixative are also fine, 
depending on what you want to see. Ethanol does not give good results and 
picric acid might cause problems during polymerization.

So I hope this helps you a little bit.

With best regards
Michael





> Am 27.04.2018 um 17:08 schrieb Thomas, Nancy via Histonet 
> :
> 
> I would like to hear from anyone with experience in working with the 
> pineapple sponge and other similar types.  A future project will include 
> processing, sectioning and staining of the samples in order to identify them 
> by viewing the arrangement of the spicules.  I am wondering if cryo, paraffin 
> or plastic would be the best method?  How much fixation is necessary?  If 
> anyone has working protocols they would share, I would be so thankful.
> 
> 
> Nancy Thomas
> 
> Senior Lab Manager, Histology Core
> 
> Stowers Institute for Medical Research
> 
> 1000 E. 50th Street
> 
> Kansas City, MO  64110
> 
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

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Re: [Histonet] Autopsy Saws

[Michael Gudo (Morphisto GmbH) via Histonet]

Hello Sandra,

if you need very high quality pathology saws we recommend the machines from 
EXAKT. We have three of them in our lab and use them for pathology sectioning 
as well as for cutting grinding techniques. They work with a diamond band and 
there are several kinds of diamond bands available.

The website of EXAKT you can find here:
http://exaktusa.com/pathology-equipment/

You can contact Linda Durbin (linda.dur...@exaktusa.com), I think she can give 
you a local contact.

With best regards
Michael

> Am 17.04.2018 um 21:54 schrieb Sandra Cheasty via Histonet 
> :
> 
> Hello all,
>We need to replace two of our Mopec saws. We perform 
> necropsies on many large species, (bovine, draft horses, zoo animals), and 
> are looking for a vendor who can give us a deal on the purchase of two sturdy 
> autopsy saws that will stand up to heavy use. We are a veterinary teaching 
> hospital, and our budget is limited.
> Cheers!
> Sandy
> 
> Sandra J. Cheasty, HT (ASCP)
> Histology & Necropsy Supervisor
> UW-Madison, School of Veterinary Medicine
> 
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder 
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Re: [Histonet] Deplastifying MMA

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hi Dorothy,
Hi Reuel,

MMA embedding and deplastination is of course a very difficult process and many 
protocols are discussed, but only a few work well. So in general: real 
deplastination cannot be performed by xylene or acetone alone, these solutions 
are just a pretreatment. The best way for remove MMA from section is a 
procedure starting with xylene, then going into acetone (2 times) and then into 
MEA (Methoxyethylacetate) for at least 2-3 times for a minimum of 10-20 
minutes. You can also directly go into MEA, however, pretreatment with xylene 
or acetone helps to reduce the steps and volume of MEA you need.

Which kind of MMA do you use?

In our lab we do resin embedding in Technovit 9100, which is very well 
established MMA that provides good results for thin sections between around a 
few microns, but and also for thicker sections and even for 
cutting-grinding-techniques. 

We can also provide you with several protocols for infiltration, deplastination 
and staining of Technovit-sections.
You find some further information and also some hands-on-descriptions for all 
histology-technovits in this brochure:

http://www.morphisto.de/fileadmin/pdf/Brochure_Technovit_Histology_EN.pdf 


If you have any further questions, just contact me personally.
With best regards
Michael

P.S. Further information about the technovits you can also find here:
http://www.morphisto.de/en/resin-histology/technovit-resins/ 



> Am 14.02.2018 um 20:45 schrieb Dorothy Hu via Histonet 
>  >:
> 
>> 
>> Hi  Reuel
>> 
> 
> We had the same thing as you. It was caused by bad MMA, I tested. Write
> down the lot # and ask refund. Test new lot # 1st of MMA in the future. It
> was very frustratingI know.
> Best luck.
> 
> Dorothy
> 
> 
> 
> 
> 
>> Message: 2
>> Date: Mon, 12 Feb 2018 21:07:15 +
>> From: Reuel Cornelia > >
>> To: "histonet@lists.utsouthwestern.edu 
>> "
>>> >
>> Subject: [Histonet] Deplastifying MMA
>> Message-ID:
>>> namprd17.prod.outlook.com >
>> 
>> Content-Type: text/plain; charset="iso-8859-1"
>> 
>> Hello histonetters,
>> 
>> I have a problem with my MMA plastic section on my slide. I could not
>> remove the plastic MMA even if I leave the section slides in Xylene for
>> several days with heat incubation at 60 degrees. This is the first time
>> that happened to my section for several years of doing the same thing over.
>> I was thinking that it was my Xylene lot number so I tried to change it but
>> still does not remove the plastic, and then Itried different solvent
>> Acetone, and Ethylene glycol Monoethyl ether but still it does not work.
>> Can I ask for help if anyone knows what was going on and what would be the
>> best way to remove this plastic from my section?
>> 
>> Just to give you my embedding solution was MMA -94% , dibutly phthalate-
>> 5% and perkadox 16- 0.5%. This have been my solution for years and I do not
>> have any problem with the removal of plastic section until now. I was
>> thinking that my MMA (M55909) different lot number from Sigma Aldrich may
>> have cause this because even I tried to use the MMA to dissolve my plastic
>> it does not work.
>> 
>> 
>> Thank you for and any opinions or protocols are greatly appreciated.
>> 
>> 
>> Reuel Cornelia
>> 
>> TSRH
>> 
>> 214-559-7766
>> 
>> 
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

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Re: [Histonet] Elastic stain

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Dear Mama,

there are several elastic stains which are as brilliant as Verhoeff.
For a very quick and easy stain you can try:

Van Gieson with Resorcinfuchsin or
Resorcinfuchsin with Thiazin-picric-acid

Also very interesting results can be generated with:

Aldehydfuchsin-Tripple Stain
and
Mollier-Staining with Orcein

You should find the protocols in standard literature like Bancroft or Romeis, 
but please allow that I insert some links to our webshop where you can buy 
these staining-kits ready-to-use:

https://webshop.morphisto.de/faerbekit-elastica-nach-van-gieson.html
https://webshop.morphisto.de/faerbekit-resorcinfuchsin-mit-thiazinrot-pikrinsaeure.html
https://webshop.morphisto.de/faerbekit-aldehydfuchsin-dreifachfaerbung.html 

https://webshop.morphisto.de/faerbekit-vierfachfaerbung-nach-mollier.html

If you have any further questions, please just contact us via email.
With best regards
Michael



> Am 06.11.2017 um 17:12 schrieb Nirmala Srishan via Histonet 
> :
> 
> Histonetters,
> 
> Is there someone who can recommend a Elastic stain other than Verhoeff?
> 
> Thanks
> Mala
> 
> 
> 
> 
> 
> 
> 
> 
> 
> Holy Name Medical Center is ranked among the top hospitals in the nation 
> for patient care, clinical performance and workplace excellence.
> Click here to learn more.
> 
>  Warning: The information contained in this message is privileged and 
> CONFIDENTIAL and is intended only for the use of the addressee above. If 
> you are not the intended recipient, you are hereby notified that any 
> disclosure, copying, distribution, or taking of any action in reliance on 
> the content of this message is strictly prohibited. If you have received 
> this communication in error, please notify the sender by replying to this 
> message, and then delete it from your system.
> 
> 
> 
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60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
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[Histonet] Unused TissueFaxs Analysis-System (TissueQuest & HistQuest)

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello Histonetters,

I am not sure if this question reaches the right persons here, but I try to 
place my question here:

We have purchased a complete TissueFaxs-Akquisition and Analysis-System from 
TissueGnostics a few years ago for a research project.  However, the project is 
finished for about 1 or 2 years, and now the Analysis System (TissueQuest and 
HistoQuest) will be not used here any more. I think it is not a very profitable 
to have this system here standing around, so I am wondering if there is anybody 
or any lab who might be interested in purchasing this system.

Here are the information about the two Applications:
http://www.tissuegnostics.com/en/products/analysing-software/histoquest 

http://www.tissuegnostics.com/en/products/analysing-software/tissuequest

It is installed on a HP Computer with a hardware dongle and we would like sell 
the complete system for a fair price for the PC and the licences of both 
applications.

So, if there is anybody here in this community who is interested, please 
contact me personally. I can offer you more information about the PC and the 
software version, etc. and we can also discuss a possible price.

With best regards
Michael



MORPHISTO Evolutionsforschung und Anwendung GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
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[Histonet] Unused TissueFaxs Analysis-System (TissueQuest & HistQuest)

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello Histonetters,

I am not sure if this question reaches the right persons here, but I try to 
place my question here:

We have purchased a complete TissueFaxs-Akquisition and Analysis-System from 
TissueGnostics a few years ago for a research project.  However, the project is 
finished for about 1 or 2 years, and now the Analysis System (TissueQuest and 
HistoQuest) will be not used here any more. I think it is not a very profitable 
to have this system here standing around, so I am wondering if there is anybody 
or any lab who might be interested in purchasing this system.

It is installed on a HP Computer with a hardware dongle and we would like sell 
the complete system for a fair price for the PC and the licences of both 
applications.

So, if there is anybody here in this community who is interested, please 
contact me personally. I can offer you more information about the PC and the 
software version, etc. and we can also discuss a possible price.

With best regards
Michael



MORPHISTO Evolutionsforschung und Anwendung GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

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Re: [Histonet] PIG processing tissue help

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Hello,

we process heart and other biopsies of muscles in Bouin oder Bouin-Hollande 
fixative which both also work fine with IHC and give very brilliant results 
with any kind of trichrome stainings.

With best regards
Michael





> Am 06.09.2017 um 18:22 schrieb Blanca Lopez via Histonet 
> :
> 
> Hello Histonettes,
> I am looking for a better information on what is the best way to treat, fix 
> and process pig heart. This is a very new thing for me because I usually work 
> with mouse and human tissue. Is anybody there that can help me with this 
> please? I will appreciate any feedback event to do perform special stains and 
> IHC for this project,
> Thanks
> 
> 
> 
> UT Southwestern
> 
> 
> Medical Center
> 
> 
> 
> The future of medicine, today.
> 
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MORPHISTO Evolutionsforschung und Anwendung GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder 
dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der 
unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe 
des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene 
Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit 
dem Absender der Email in Verbindung zu setzen und anschliessend diese Email 
und saemtliche Anhaenge zu loeschen.

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