[Histonet] Farewell
Good morning Histonetters, Well, the time has come to say farewell to Histonet and all the people it represents. I've appreciated the invaluable information, humor and encouragement that has been available here. I hope I've been able to help out people now and then as well. I've never regretted entering the field of histology 40 years ago. I started out in clinical work, followed by two stints in research and finished by helping start the immunohistochemistry/in situ hybridization lab that I work in today. I've always been able to keep the patients we serve in mind as I've gone about my daily work. That's what's made this work so satisfying. I leave you all with the wish that you continue to find purpose and satisfaction in the critical work you do. Take care, Linda A. Sebree, HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Reference lab offering ARID-1a on FFPE tissue?
Sorry, I meant ARID-1a. From: Sebree Linda A Sent: Friday, October 23, 2015 9:17 AM To: Histonet (Histonet@lists.utsouthwestern.edu) Cc: Weisman Paul Subject: Reference lab offering ARIDIA on FFPE tissue? Hello Histonetters, Anyone know of a reference lab offering this antibody? Thanks, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reference lab offering BRAF by IHC on FFPE specimens
We're looking for a reference lab that performs the above immunohistochemical stain. Thanks, Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reference lab offering ARIDIA on FFPE tissue?
Hello Histonetters, Anyone know of a reference lab offering this antibody? Thanks, Linda Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana antibody - H pylori
We have excellent and very clean results with the VMS H. Pylori on our Ultras. I can shoot you our protocol if you're interested. Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: Histology Technician [mailto:histology81...@att.net] Sent: Thursday, July 09, 2015 3:51 PM To: Histonet; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] Ventana antibody - H pylori Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved. Any thoughts? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana antibody - H pylori
Rabbit monoclonal, Richard. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: Cartun, Richard [mailto:richard.car...@hhchealth.org] Sent: Thursday, July 09, 2015 4:06 PM To: Histology Technician; Histonet; histonet-requ...@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventana antibody - H pylori Is the Ventana antibody polyclonal or monoclonal? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: Histology Technician [mailto:histology81...@att.net] Sent: Thursday, July 09, 2015 4:51 PM To: Histonet; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] Ventana antibody - H pylori Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain? My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved. Any thoughts? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Ventana LCS (Liquid Cover Slip)
You certainly can air/oven dry your slides instead of running them down and coverslipping; we do that with the slides we take off in the morning when we get in. As to your problem with water/oil on your slides, something is not right in your dawn water rinsing, dehydration and/or clearing steps...but I'm sure I'm not telling you anything you don't know already. I would change out all the graded alcohols and xylenes so you know the right reagents are in your containers. Next, I would make sure that whomever is rinsing the slides, that they are using warm or even hot Dawn water and that they are rinsing long enough for your DAB slides. If you're also running APR you may want to do the air/oven drying to xylene method to maintain the chromogen binding. Other than these steps, I don't know what else to suggest. A long, long time ago when I worked in an old lab with water chilled pipes for air conditioning, I ran into a lot of humidity in the summer and actually had to add molecular sieve to my 100% alcohol in my dehydration set-up or my xylenes would get contaminated with water but we're talking the mid 70's so I'm hoping you're not running into something similar. Hope this helps, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: Burnett, Brandy [mailto:bburn...@capecodhealth.org] Sent: Thursday, July 02, 2015 12:07 PM To: Sebree Linda A Subject: RE: Ventana LCS (Liquid Cover Slip) For IHC..We are rinsing them with dawn before running them down. Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburn...@capecodhealth.org Expert physicians. Quality hospitals. Superior care. -Original Message- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Thursday, July 02, 2015 10:17 AM To: Burnett, Brandy Subject: RE: Ventana LCS (Liquid Cover Slip) Are you talking about IHC/ISH or Specials Brandy? Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: Burnett, Brandy [mailto:bburn...@capecodhealth.org] Sent: Thursday, July 02, 2015 7:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana LCS (Liquid Cover Slip) Anyone out there having any issues with the Ventana LCS? When you are cover slipping are you seeing any water/residual LCS on the slides? I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated! Brandy Burnett Histotechnologist, QIHC(ASCP) CCH Pathology/Histology bburn...@capecodhealth.orgmailto:bburn...@capecodhealth.org ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC turnaround time
We have 2 FTEs in IHC; myself as one of two people that started the lab 20+ years ago and 2 histotechs that rotate through on a weekly basis. Our monthly totals currently run between 2400 and 3300. Our TAT policy is in by noon, out by 5pm except for dual and triple stains (need to be on an instrument by 11:00 am) and EBER (needs to be on by ~ 9:00 am) and HER2 dual ISH (only run overnight). We have 4 Roche (Ventana) Ultras that hold 30 slides each and are continuous access. All instruments are used every day and at least one (usually 2 or 3) in use overnight. Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: Cartun, Richard [mailto:richard.car...@hhchealth.org] Sent: Friday, June 19, 2015 10:21 AM To: Olszewski, Dawn; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC turnaround time I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs who spend most of their time doing IHC, but they also help Histology embed and cut paraffin blocks when needed (which is most days). For 2015 we are averaging 4,022 IHC patient test slides and 1,650 IHC control slides per month. Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload). We have 5 Leica Bond Max platforms; however, we still do some IHCs on the bench. We usually do 2-3 runs per day and an overnight run (except on Fridays) as well. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax -Original Message- From: Olszewski, Dawn [mailto:dawn.olszew...@sgmc.org] Sent: Friday, June 19, 2015 10:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC turnaround time Hi Histonetters, 6-19-15 We are wondering what the average turnaround times for IHC are for other labs. We use the Biocare Intellipath instrumentation. It holds up to 50 slides ( 10 slides per 5 racks) with continuous feed per open rack. All IHC orders placed by 12pm are usually out the same day by 5pm. We average 434 IHC slides per month and have a staff of 3 FTE's. A pathologist has voiced concerns over our IHC output. We are trying to determine best practices for IHC turnaround time as measured from time order placed to time of slide delivery. If you could respond with your IHC TAT including number of techs and average of IHC slides monthly, we would appreciate any input you may be able to provide. Thank you in advance, Dawn Olszewski, HTL(ASCP)QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: IHC and Oven Temperatures
That's the reference I have always gone by since I first started doing IHC back in 19.. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of richardb...@charter.net [richardb...@charter.net] Sent: Sunday, April 26, 2015 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: IHC and Oven Temperatures The specific pages in the Dako Education Guide: Immunohistochemistry Staining Methods, Fifth Edition are: Discussion on page 32 and references on page 33. It's in the Fixation and Processing Chapter and says no part of the process should have temperatures above 60C. Rick Boen, BS, HTL (ASCP) Histology Lab St. Luke's Hospital Duluth, Mn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC/SS QA/QC Sheets:
Hi Craig, As technologists, we of course QC all slides before they get to the pathologist(s) but that is not documented unless we put a repeat order in the case. Then we add a note saying why a test needs repeating, i.e. positive control did not stain, patient tissue loss, etc. But this is all done electronically, so no paper trail. For general QC, we have gone to having a statement automatically populate to any case reports that include IHC/ISH staining but I'm assuming this could also work for HEs, Special Stains, etc.; I'm only familiar with the IHC/ISH portion of QCing. Our statement says something along the lines of the controls staining appropriately and as expected. The pathologist that signs out the case is attesting to that statement. This statement has eliminated HUGE amounts of paper and filing. Hope this helps, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Tuesday, April 21, 2015 3:16 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC/SS QA/QC Sheets: Can someone help guide me on the right direction regarding how to organize daily QC sheets. Currently we have one per case (this is time consuming and I'm on overload of papers). Does anyone have a good solution and are you willing to share? Thank you for your help, Craig Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] C3d?
Cleveland Clinic does it by IHC and I think also by IF. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Wednesday, April 01, 2015 6:14 PM To: HistoNet Subject: [Histonet] C3d? Hello Again My Dear Netters, One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain? If so what type: IHC, IF? Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HNF-1 beta
Good morning, We have a pathologist that has a case he wants sent out for HMF-1β (hepatocyte necrosing factor-1β) . Are there any reference labs out there that offer this? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16 Antibody
We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HER2 IHC controls
Yes, 3 different antibody expressions per block. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Tuesday, January 06, 2015 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 IHC controls Are any of you making your own HER2 IHC control slides from patient tissue? ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] 2 Sucinyl Cystine stain (AKA 2SC)
Good morning, Does anyone know of a reference lab offering this IHC stain for human FFPE tissue? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC Validation
Karla, We try to run 1/2 of the required cases with known negative cases and the other 1/2 with known positive cases. In addition, all cases are run with a negative reagent control in place of the antibody using the optimized protocol for the antibody. These are your negative reagent controls. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A Sent: Tuesday, November 04, 2014 10:27 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] IHC Validation I am validating IHC's. What is the CAP requirements regarding negative slides. I've asked CAP but have not received a response. Are 10 negative patient tissues ran for each antibody using the negative protocol or are they ran using each antibody but also a known Negative control ran using the negative protocol? Thanks! Karla Arrington, HT (ASCP) HIT (AHIMA) Supervisor of Pathology ANMC Pathology kaarring...@anthc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fibronectin
Does anyone know of a reference lab doing this antibody on human FFPE slides? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Salary exempt/nonexempt status
I fought this very same battle having been salaried for many years and gradually working more and more OT. I worked solely with hourly employees and was not a manager or supervisor. Everyone I worked with accrued OT pay but I did not and I was usually working the most OT. I contacted our state office of labor and workforce development and learned about the exempt/nonexempt statuses. Upon bringing this information to both my institution's personnel department and eventually my manager, they reviewed my position description along with several other employees. They resolved the issue by making me and some other people hourly with no cut in pay so now I still work some overtime but accrue OT pay. I also received back pay from 2 years of overtime. Maybe CA has a labor department that could help you as well. Good luck, Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of GMail [nguy0...@gmail.com] Sent: Saturday, October 25, 2014 3:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Salary exempt/nonexempt status Can someone please help me, I've been losing sleep over this ever since moving to CA and working here. If you are paid a salary but NOT a supervisor or manager are you considered exempt or nonexempt? I was told by someone that if you are salary but not in a managerial position you are considered nonexempt and are entitled to earn OT. I have tried searching the laws in CA and the only thing I could find is: 1. If you earn twice the wage of minimum wage you are exempt. 2. If you are a type of professional (doctors and lawyers etc) or in a managerial position you are exempt With that being said, I believe a lot of people in the U.S with other professions make twice the wage of #1, so does that mean they are all exempt? Please help me answer this question! Thank you in advance! Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Another TGIF Friday...
This is by far my favorite! Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Weems, Joyce K. [joyce.we...@emoryhealthcare.org] Sent: Friday, October 10, 2014 11:35 AM To: 'sarah.dys...@stdavids.com'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Another TGIF Friday... LAB HIERARCHY Chief Pathologist Leaps tall building in a single bound Is more powerful than a locomotive Is faster than a speeding bullet Walks on water, Gives policy to God Associate Pathologist Leaps short buildings in single bound Is more powerful than a switch engine Is just as fast as a speeding bullet Walks on water if sea is calm Talks with God The Department Head Leaps short buildings with a running start and favorable winds Is almost as powerful as a switch engine Is faster than a speeding bullet Walks on water in an indoor pool Talks with God if special request is approved Director of Laboratories Rarely clears a Quonset hut Loses tug of war with locomotive Can fire a speeding bullet Swims well Is occasionally addressed by God Associate Director of Laboratories Makes high marks on the walls when trying to leap tall buildings Is run over by locomotives Can sometimes handle a gun without inflicting self-injury Dog paddles Talks to animals Supervisor Runs into building Recognizes locomotives two out of three times Is not issued ammunition Can stay afloat with a life jacket Talks to walls Chief Technologist Falls over doorstep when trying to enter building Says look at the choo-choo Wets themselves with a water pistol Plays in mud puddles Mumbles to Themselves Histotechnologist Lifts tall buildings and walks under them Kicks locomotives off the tracks Catches speeding bullets in their teeth and eats them Freezes water with a single glance They are God Anonymous Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Friday, October 10, 2014 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Another TGIF Friday... Does anyone have that thing handy that says what different positions in the histology lab do? It's like Superman, leaping buildings and such... Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Negative Control for Validation
Jamal, We use the same patient cases throughout the validation procedure. So each block gets stained with the new antibody (optimized protocol already signed off by IHC director or designee), vimentin (+ reagent control) and negative control serum (- reagent control). As stated earlier, the negative tissue control is any non-staining negative tissue elements, expected to be negative, within the block. If you're interested I can send you our Validation Protocol Proposal form under separate cover. Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: Jamal [mailto:j.rowa...@alborglaboratories.com] Sent: Tuesday, September 30, 2014 5:15 AM To: Sebree Linda A; 'Arrington, Karla A'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Negative Control for Validation Hi Linda can you give us more details about the controls selection and processing procedures. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Tuesday, September 30, 2014 12:43 AM To: 'Arrington, Karla A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Negative Control for Validation Hey Karla, We use Vimentin as a positive reagent control, our negative control serum for the negative reagent control and for the negative tissue control, the pathologist reviews the antibody stained slide and OK's the internal negative tissue elements as appropriately negative. Hope that helps, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A Sent: Monday, September 29, 2014 1:25 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Negative Control for Validation We are beginning the process of validating new antibodies using the BenchMark XT but have some questions for validation. Question: For a positive reagent control that stains appropriately positive, is a known control block used? If not, what is used? Question: For a negative reagent control that stains appropriately negative, what is ran? Question: Is a negative tissue slide ran for all of our 10 cases per antibody, or per case that will be used for validation? Thanks All! Karla Karla Arrington, HT(ASCP), HIT(AHIMA) Lead Histology Technician ANMC Pathology 907-729-1810 kaarring...@anthc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Light/Heavy chain Myosin to differentiate Type 1 vs Type 2 muscle fibers
Good morning Histonet, We are looking for a reference lab to send FFPE slides to for the above antibody(ies). We sent out for this test several years ago but of course at that time our records were very rudimentary, i.e. log books, so difficult to go back and search through. Hope someone knows something to help us. Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: FDA Disclaimer
Well put Tim. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, September 24, 2014 1:24 PM To: 'Arrington, Karla A'; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: FDA Disclaimer Karla, the thing is, they may be called IVD under FDA nomenclature, but they are not all FDA approved. Class I exempt antibodies (the vast majority) don't need FDA approval because they are ancillary tests used in conjunction with other tests to arrive at a Dx. Only stand-alone tests like ER, Pr, Her2 and EGFR are in Class II and require FDA approval. An FDA certified company can list any antibody (except the stand alones) as IVD Class I simply by submitting a list to FDA. The company must have FDA approved quality controls in place and follow a slew of regulations to do so, but it is simply a paperwork exercise after that. So, the disclaimer you mention says that the antibody in question is not FDA approved, but is not required to be FDA approved. The CAP and ASCP suggested this disclaimer so that customers would be made to understand that although the tests are not under FDA approval it does not mean they are not valid tests. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Arrington, Karla A Sent: Wednesday, September 24, 2014 10:44 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] FDA Disclaimer To All: We are starting up IHC at the lab and I have a question about disclaimer. If we are using all FDA approved antibodies, do we need the FDA Disclaimer on our pathology reports? And if so, what should it say? Karla Arrington =) Karla Arrington, HT(ASCP), HIT(AHIMA) Lead Histology Technician ANMC Pathology 907-729-1810 kaarring...@anthc.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Automated Special Stainer...
We've had VMS instruments from day 1 and currently have 4 Ultras. The random access capability is awesome and we can even get out EBER ISH slides the same day if put on in the morning. Our HER2 dual ISH stains run overnight as they are 14 hour protocols. Reproducibility among the 4 instruments and concurrent/consecutive stains on the same instrument are a given. Technical advice and service are outstanding. And, no, I do not have a stake in the company just am really sold by their products. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Wednesday, September 17, 2014 12:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Brian wrote: ... one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it. We are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow. We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, September 17, 2014 10:33 AM To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Dako Artisan all the way! I've used both systems (the Artisan in my old institution). We currently have a few of the new Benchmarks on demo right now. Thus far, we're not that impressed. Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). Oh yeah, one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. You'll need two Benchmarks to do what one Artisan does, TATs being equal. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, September 17, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special Stainer... Opinions on Ventana versus Dako...Go! Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] RE: Automated Special Stainer...
Sorry, I thought we were talking IHC/ISH not SS. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Cooper, Brian [bcoo...@chla.usc.edu] Sent: Wednesday, September 17, 2014 2:36 PM To: Morken, Timothy; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... I guess TAT's being equal is a relative term, depending upon your workload. For our purposes, it's not slide capacity that poses a problem--it's reagent capacity. We've never loaded 50 special stains at any one time--we typically don't even have half that amount in one day! So here's a scenario common to the workload at our institution. For our liver panels, we run Iron, Trichrome, Retic, PAS with and without Digestion. That pretty much maxes out the reagent capacity on the Benchmark. Sure, we can load up to 20 of these onto the Benchmark. But as is so frequently the case, we also have just 1 GMS that also needs to be stained! This is where the second Benchmark comes into play, or we'll have to stain by hand. One Artisan can handle this at the same time, and if memory serves (disclosure--it's been about 2 years, and the model I used didn't have online deparaffinization), it didn't add all that much time to the process. It was certainly faster than waiting for the machine to complete, and start another run. For our institution, it's either going to be 2 Artisans or 2 Benchmarks. The Artisans will give us some greater flexibility due to the greater reagent capacity. Brian -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, September 17, 2014 10:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Brian wrote: ... one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. Are the TAT's equal? When we loaded up our old artisan with slides (50) and full of reagents it took over 5 hours to finish - way past our deadline. So nobody wanted to use it that way so the techs resisted and wanted to do manual to get the slide out. We ended up putting only a few stains on it. We are looking at doing specials the way we do immunos - on demand all day rather than in one batch in the AM. It seemed to me that the 20-slide units from Ventana allow small batches and run in parallel or allow staggered use. Buying a 50-slide capacity instrument and then putting only 20 slides at a time on it seems odd somehow. We looking at each of these as well and not sure yet which would serve us better. We do about 60-80 specials slides per day, and 13 stains give us 90% of our volume. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cooper, Brian Sent: Wednesday, September 17, 2014 10:33 AM To: sarah.dys...@stdavids.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Automated Special Stainer... Dako Artisan all the way! I've used both systems (the Artisan in my old institution). We currently have a few of the new Benchmarks on demo right now. Thus far, we're not that impressed. Frequently, there's variability on the silver stains (even in the same run,) and adjusting timing is nowhere near as flexible as on the Artisan. Even if you take away the half hour for online deparaffinization, the stains take significantly longer on the Benchmarks (than the old Nexes we are still running in our lab). Oh yeah, one more thing. The reagent tray capacity on the Artisan is about twice what it is on a Benchmark. You'll need two Benchmarks to do what one Artisan does, TATs being equal. Brian D. Cooper, HT (ASCP)CM | Histology Supervisor Department of Pathology and Laboratory Medicine Children's Hospital Los Angeles 4650 Sunset Blvd MS#43- Los Angeles, CA 90027 bcoo...@chla.usc.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, September 17, 2014 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated Special
RE: [Histonet] C-Myc
We do. I'll email Tuesday when I'm back at work. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Rae Staskiewicz [raest...@grics.net] Sent: Friday, September 12, 2014 3:33 PM To: Histonet Subject: [Histonet] C-Myc Would anyone have a protocol for C-Myc using the Ventana Benchmark Ultra or XT? Thanks in advance for any information you can provide. Rae Ann Staskiewicz UnityPoint Health Methodist Peoria, IL 309-672-5994 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC Validation:
I think your last question says it all; it ultimately is up to the director although she/he better be able to explain to a CAP inspector why it was done the way it was done. We generally follow the CAP guidelines with 10 - 20 +/10-20 - cases. We've also used the internal negative elements as being negative as expected in our validation documentation. In some cases, when + cases are rare we've used less than the suggested number just as CAP says is acceptable but we've usually been able to come up with enough. TMAs are a great way to go if you have them or can make them; cuts down on labor/reagent cost. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Jb [craiga...@gmail.com] Sent: Friday, August 29, 2014 12:29 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Validation: How do most people validate IHC? I want to create a tissue microarray. I wanted to use an average of 6-8 positive tissues. Is it necessary to validate using negative tissues when there is always an internal negative control in all tissue sections. Now with new polymer detection systems there is not background, etc. Is IHC validation ultimately up to the discretion of the laboratory director? Please advise. Thx- Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC Validation:
Well said Joelle; we do pretty much the same. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Joelle Weaver [joellewea...@hotmail.com] Sent: Friday, August 29, 2014 4:09 PM To: Jb; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC Validation: Yes, ultimately up to lab director/medical director/pathologist as to determination of specificity, selectivity, and if you have enough examples, and the staining reactivity conforms to the intended clinical use during their assessment and hopefully approval of your protocol. I have used some TMAs with success, especially if you make with your own in-house processed tissues. I try to strongly favor using several expression levels of normal and diseased tissue whenever possible that reflect what it will be used for in the patient test tissues. If single sections, I try use both expected or known negative and positive tissue both normal and diseased , when practical for the first validation set when I get past optimization. For small adjustments I may need only a few more confirming positives- up to MD in my situation. I also have polymer detection, but I still like some negatives for me. Some people may not feel this is necessary, and the pathologist may not need the negatives ( using internal controls), but this helps me, so I do it to feel more confident in my results as I present the slides for review. I don't see why you couldn't use internal negatives, if you clarify what tissue element acts as the internal negative in the tissue type in the validation summary and SOP. Basically, for amount or # to stain, I follow the CAP guidelines ( newer ones), for well characterized. For markers with specific guidelines for validation and correlation, I follow the CAP guidelines exactly. Setting up the process/SOP s, I used the CLSI guidebook on validation of IHC assays. Both resources (CAP CLSI) have been very helpful for me. That is what has been working for me, I hope this helps. Joelle Weaver MAOM, HTL (ASCP) QIHC From: craiga...@gmail.com Date: Fri, 29 Aug 2014 10:29:15 -0700 To: Histonet@lists.utsouthwestern.edu CC: Subject: [Histonet] IHC Validation: How do most people validate IHC? I want to create a tissue microarray. I wanted to use an average of 6-8 positive tissues. Is it necessary to validate using negative tissues when there is always an internal negative control in all tissue sections. Now with new polymer detection systems there is not background, etc. Is IHC validation ultimately up to the discretion of the laboratory director? Please advise. Thx- Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC QC forms:
Brandy, We are completely paperless now so we created a code called RPTIHC that had no billing charge associated with it. The pathologist then puts what antibody/antibodies need repeating in the instruction box of the repeat code. They also indicate the reason for the repeat(s). It populates nicely to the worklist that gets printed; works well for us. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Thursday, August 21, 2014 12:11 PM To: Morken, Timothy; 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: Thanks for your input. We are having the same issues with our IHC QA/QC and monthly repeat count. The pathologists place the IHC orders through PowerPath, so I'm hoping that we can create a Repeat code in PowerPath to keep track of them. Do you know how it was set up in your ordering system? Thanks again, Brandy Burnett, HTL Cape Cod Hospital From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Thursday, August 07, 2014 2:26 PM To: 'Jb'; Histonet Subject: RE: [Histonet] IHC QC forms: We only check and document control slide quality and need for repeat due to control failure. The pathologist reviewing the slides will send a QC form to us if there is a problem with case slides. Plus thye can enter a repeat for a poor stain as a repeat and we can track the number of repeats in the system. It is actually one of our QA monitors and review of repeats is documented in our quarterly QA review meeting. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Thursday, August 07, 2014 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC QC forms: Currently we have a IHC QC form for every case that we run. It lists dr, antibody, date, comments, quality results. The HT and pathologist sign off on the quality. Is this necessary for every case or can we do a daily log for all cases? This process is a little time consuming. Does anyone have a good process in place that they can share? Thank you. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] On the lighter side...
38 years and looking forward to retirement in a few years. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Jamal [j.rowa...@alborglaboratories.com] Sent: Saturday, August 09, 2014 4:47 AM To: 'Vincent Rivera'; 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... Similar as me So strong, proud with high confidence Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | www.alborglaboratories.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vincent Rivera Sent: Thursday, August 07, 2014 11:58 PM To: 'Douglas Porter'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] On the lighter side... 15 Years and still going strong :) Vincent Rivera, HT (ASCP), QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vriv...@westderm.com 714-924-7240 (Lab) 714-390-0906 (Cell) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas Porter Sent: Thursday, August 07, 2014 11:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On the lighter side... How long have you been a registered histotech? 36 years here. You??? Douglas A. Porter, HT (ASCP) Grossing Technician IT Coordinator Cancer Registrar CAP-Lab, PLC 2508 South Cedar Street Lansing, MI 48910-3138 517-372-5520 (phone) 517-372-5540 (fax) mailto:doug.por...@caplab.org doug.por...@caplab.org http://www.caplab.org/ www.caplab.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Storage of HER2 neu control slides
Good morning, I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? Thanks for any and all replies, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Storage of HER2 neu control slides
Thanks Joelle. I'm surprised that Oracle is suggesting overnight baking as I noticed a huge difference when I dried some test tissue controls for 30 @ 60 degrees vs 10 @ 60 degrees. I was testing some blocks that I had 3 cores of varying degrees of intensity. All showed much decreased reactivity at 30 vs 10. In fact, the 2 weaker cores were virtually negative. I can only assume you're using a different clone than we are and that there is different epitope stability between clones. Thanks for your response, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From: Joelle Weaver [mailto:joellewea...@hotmail.com] Sent: Monday, July 14, 2014 9:25 AM To: Cartun, Richard; Sebree Linda A; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Storage of HER2 neu control slides I do the complete drain dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. Joelle Weaver MAOM, HTL (ASCP) QIHC From: richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org To: lseb...@uwhealth.orgmailto:lseb...@uwhealth.org; Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu Date: Mon, 14 Jul 2014 14:22:43 + CC: Subject: [Histonet] RE: Storage of HER2 neu control slides We keep all of our positive control slides at RT. The breast predictive marker slides often sit for a few weeks (after cutting) before being used. I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Monday, July 14, 2014 8:44 AM To: Histonet (Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu) Subject: [Histonet] Storage of HER2 neu control slides Good morning, I'd like to get some opinions on the storage of positive control slides for HER2 neu. We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind. What has peoples' experiences been with the storage of these control slides? Thanks for any and all replies, Linda Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC proficiency testing
Hi Kari, I'd suggest taking advantage of CAP proficiency testing surveys so as to compare your lab's results to others. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Delray Beach Pathology Kari Simeone [ksime...@leavittmgt.com] Sent: Monday, June 23, 2014 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC proficiency testing Can anyone share their CLIA proficiency protocols/programs for automated IHC staining? Other than the obvious antibody validation, positive and negative controls, QA and QC performed, should we enroll in an actual program for our IHC? We currently use two Leica Bonds and about 70 antibodies. No Her2 or ER/PR or FISH. Thanks in advance! K. Simeone ksime...@leavittmgt.commailto:ksime...@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Negative Controls
Tasha, We use the negative elements within our patient sample as our negative tissue control. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Campbell, Tasha M. Sent: Wednesday, April 30, 2014 6:48 AM To: Terri Braud; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Negative Controls So this has confused me more. So before you would run a negative control for each block you were testing and you would use the negative mouse or rabbit reagent. Now you don't have to do that but you still need a negative tissue control. So what exactly does this mean? Does it mean for every antibody that you are running you need to have a negative tissue control for it? So instead of using the negative mouse serum you would run a known negative tissue control with the antibody, say CD3 or whatever it is? So are most people doing a control slide with a negative tissue and a positive tissue on it? Tasha Campbell, B.S.,HTL(ASCP) Frederick Gastroenterology Associates 310 W. 9th St. Frederick, MD 21701 301-695-6800 ext. 144 (w) 304-685-9307 (c) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, April 29, 2014 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Negative Controls On Message 7 - Negative Controls While it is true that if you run polymers, you no longer have to run a negative reagent control, HOWEVER, you still must have a negative tissue control, which to quote CAP: must show no staining of tissues known to lack the antigen Any of the following can serve as a negative tissue control: 1. Multi tissue blocks. These can provide simultaneous positive and negative tissue controls and are considered best practice... The type of negative tissue control used (i.e. separate sections, internal controls, or multitissue blocks) must be specified in the laboratory manual. Thus sayeth CAP, the almighty. Please see ANP.22570 Our lab has defined our negative controls as a piece of Uterus as the negative tissue in a multitissue block as a negative tissue control for most of our antibodies, though for a few that might be too reactive in uterus, we use a piece of skin. I hope this helps. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 Message: 7 From: Beth Brinegar bbrinegar...@gmail.com Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Negative controls
We still run negative controls. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Brinegar Sent: Monday, April 28, 2014 4:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative controls Hello fellow histonetters, What is are other labs doing to satisfy the ANP.22570 QC - Antibodies Appropriate negative controls are used. I apologize, I know this question has been asked before. I'm trying to satisfy these requirements in one procedure. Thank you all for your assistance!! Beth Brinegar HTL(ASCP) Anatomic Pathology Supervisor Mercy Medical Center Cedar Rapids, IA 52403 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: MUM-1
This is our protocol for the Ultra; maybe it will help. 64 CC1, 32 incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4. This is with UltraView DAB detection. We use tonsil as well however we validated with HD, LN, GI, tonsil, etc. Good luck! Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie Sent: Thursday, March 13, 2014 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MUM-1 Good morning Histonet Folks, I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the usual adjustments and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this. Cassandra Davis cda...@che-east.org 302-575-8095 Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Mailing Freebie
I was told they're in the mail. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa Sent: Friday, March 07, 2014 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mailing Freebie OK - I may be missing something, but what is that blue adhesive pocket that was sent out with histology professionals' day literature? Just curious . . . . . Happy Friday. Tresa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Negative Controls for IHC
Hi Tanya, We have made the decision to continue running negative reagent control slides. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, February 11, 2014 12:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Negative Controls for IHC I just read in Advance Dec 2013 that there is a possibility of laboratories utilizing fewer QC controls to cut costs? Has anyone stopped running negative controls for IHC? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Lysol I.C.
That's what we use Anita and we purchase it from Cardinal as well. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita Sent: Thursday, January 23, 2014 10:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Lysol I.C. We are using Lysol I.C. for disinfecting the ventana benchmark. I thought I purchased it from Cardinal but I'm not sure now where we got it. Are others using this for their machine and where are you purchasing it from? Thanks so much. Anita Dudley Providence Hosp. Mobile, Al. 36695 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Beta-Amyloid antibody, clone 6E10 availability
So Histonet land, our usual vendor for this antibody can no longer get this clone. My question is: does anyone know who might hold the patent on this clone or at least know any company selling the 6E10 clone? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ventana Labels
We tried another vendor for ebar labels, FloPro Tek, easternlabsvc.com, but they didn't hold up in xylene. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Christopher Jacobs Sent: Thursday, December 26, 2013 12:39 PM To: 'histonet@lists.utsouthwestern.edu' Cc: Irene G. Campbell, HT (ASCP) Subject: [Histonet] Ventana Labels Histonetters, Like everywhere else, my lab is trying to find ways to save money. One thing we are looking at are the labels/ribbon we use for our Ventana EBAR printers and our Ventana Vantage Zebra printers. Currently, we order labels/printer ribbons for both types of printers directly from Ventana. As one can imagine, we are paying a premium price. Has any out there had any luck finding labels/ribbon cheaper from other sources? Thank you everyone! -CJ CONFIDENTIALITY NOTICE: This message and any attachments are for the sole use of the intended recipient(s). This message is confidential and may also be privileged. If you are not the intended recipient, please contact the sender immediately, delete the contents of this message and do not use it for any purpose. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: On-slide IHC control workflow?
This is what we've done when we recently moved to control tissue on every slide for IHC as well as Special Stains. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, December 04, 2013 11:46 AM To: Histonet Subject: [Histonet] On-slide IHC control workflow? We are planning our move to using on-slide controls for IHC and I'm wondering how other labs handle the workflow and logistics of matching controls to stain orders. We plan to use a TMA for 80% of our orders. So far we have one TMA that covers most Ab's but the number will probably will be expanded (neuropath-specific, Hempath specific, etc). For those who do this now, how do you handle these tasks: * Do you use TMA or single-tissue controls? Or a mix? We use single tissues for most and have decreased the number of different tissues being used as controls to a more generic tissue that will cover a wider range of antibodies. * Cutting all the controls - one or more techs? Part of normal work? Outsource? (especially TMA cutting - inhouse or outsource?) Cutting controls is a component of several of the histologists' rotations so every week at least 2 people have this task as part of their rotation. * How do you store the controls? (We plan to cut nearly just-in-time, maybe two days from use). Most are stored at RT; some are stored at - 20 degrees C for those antigens that may lose reactivity at RT * How do you distribute to the cutters? The IHC lab prints control slides and pulls the corresponding blocks to have at the ready for those people on the rotations that include cutting controls...they come pick the slides blocks up when they're ready to cut * How do you Indicate to the cutting techs which control slide to use for a particular stain? (we use over 200 antibodies so need to make as easy as possible without memorization) We have a master list of our antibodies posted where IHC slides are organized, that indicates which control tissue to use for each antibody. * How do you prevent the wrong control slide from being used? People learnif a mistake is made, that person is written up so the importance of paying attention to detail is reinforced. Anything else we should consider? We cut large amounts, i.e. 200 tonsil controls, at a time rather than a couple days before the need for control slides. These are kept in slide files in the area of IHC slide organization where the barcode labels are printed for use on our IHC stainers. The control slides are then labeled along with blank slides for the negative controls and taken to microtomy work stations ; works well for us. Thanks for any help!! Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.353.1266 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: CAP survey Question
We also have Ultras Jim. We don't print out the run logs. IHC personnel review the controls before they go to a pathologist in order to catch any problems. The pathologists are supposed to review the controls associated with each case they sign out. Our pathology report has a statement included that the negative and positive controls have stained appropriately. By signing off on each case, the pathologist is attesting to the fact that he has indeed reviewed the controls...we do not police them. So far, this works for us. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Wednesday, December 04, 2013 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP survey Question For a long time I have had our IHC techs print out the run logs from each IHC run on the Benchmark Ultras. The techs then check the slides to make sure the positive and negative controls have worked properly before the slides are sent to the individual pathologists. The pathologists are also supposed to check the controls before looking at the patient slides. Lately in the interest of reducing turnaround time I have been asked why we run the log reports and have a tech look over the controls before they send them to the pathologists since the pathologist will also evaluate the controls. I have been doing this because I wanted the documentation that someone reviewed the controls each time an IHC stain was done. I believe if the pathologists would document someplace that the control slides were reviewed before the patient slides were viewed then I could eliminate the techs looking over the controls also. Problem is how are others documenting that the controls are reviewed? Is this done by the techs, the pathologists, or both? We of course have also used this data for quality assurance of our stains. Thanks for your help. Jim James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: AA Amyloidosis
We sent part of what we had to ProPath so if you need a reference lab to send to, they offer this IHC test. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Obregon, Cecilia Sent: Monday, November 18, 2013 3:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] AA Amyloidosis Histoneters, Does anyone have a positive block (or two) for AA Amyloidosis? I'm willing to trade for other hard to find controls. Let me know. Thank you, Cecilia M Obregon Memorial Regional Hospital 3501 Johnsonn Street Hollywood, FL 33021 CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER. This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Validating on new Benchmark
Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar Sent: Friday, October 11, 2013 6:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Validating on new Benchmark Hi everyone, I have a few more questions that I was hoping I could get some help on. 1. How did everyone go about obtaining the positive and negative controls-(did you choose patient cases or go with generic controls such as extra tonsil, appendix, colon,etc...) We used both. 2. For the negative controls do you have to find separate negatives or can you use the internal negative controls if the tissue has it. Our in-house QA people determined we need to have separate negative cases. 3. Do you have to do a minimum of 10 positive and negatives or can we do 5 (other than the ER/PR and Her2) CAP is vague on this saying that for hard-to-find positive cases, less than 5 +/- will suffice. Thank you Fawn - This electronic message may contain information that is confidential or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute, or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Negative Reagent Controls in Diagnostic IHC....
Richard, I wish we could eliminate them but not only do our negatives displaying some non-specific staining, seems to be tissue dependent, but our in-house QA people say we need to continue using them per the data sheets accompanying many of our antibodies. We use Ventana UltraView DAB detection, a multimer kit. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Wednesday, October 09, 2013 3:59 PM To: Histonet Subject: [Histonet] Negative Reagent Controls in Diagnostic IHC My colleagues and I presented a poster at the NSH annual meeting in Providence recently titled, Negative Reagent Controls in Diagnostic Immunohistochemistry: Do we need them?. I have received a few requests for the actual poster (PowerPoint slide). I will be happy to e-mail it to anyone who is interested. Oh, by the way, we have determined that they are not needed in our laboratory and by eliminating them we have saved our laboratory over $100,000 a year! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] C3d by IHC on FFPE human specimens
Does anyone in Histoland know of a reference lab that offers this? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC trun-around-time
Hey Tom, Although we have a higher workload than you, ~ 1500 slides/month, we offer same day TAT with a cut-off of noon. There are a couple exceptions, i.e. triple stains and HER2 dual ISH but otherwise, utilizing 4 Ventana Ultras, we have it all out by 4 pm or slightly later. Our hours are 5 am to 4 pm with 2.25 FTEs. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Tuesday, October 01, 2013 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC trun-around-time Hello all, I am wondering how many places offer same-day staining of IHC. We are a small hospital based lab that averages about 70 IHC slides per week. We operate 5 days/week, 0600-1600. We give same day staining for IHC requests that are received by 10am and are using the Ventana Benchmark XT. Due to some issues that have developed, we may have to change the way we do things. With our current 10am cutoff plus possible pre-analytical changes the instrument will finish too late in the afternoon. The pathologists really like the same day service on IHC but I'm thinking that I may have to make the cutoff time earlier and anything after that will just have to wait. So I am wondering if anyone else of similar size, instrumentation, and workload, offers same-day staining for IHC. I appreciate your input. Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.orgmailto:tmcne...@lmhealth.org www.LMHealth.orgfile:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Unregistered HT
Well said Jean. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mitchell Jean A Sent: Wednesday, September 11, 2013 9:26 AM To: 'Horn, Hazel V'; 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Even if a facility does not require that techs be HT registered - the technicians themselves need go that extra step, take the initiative themselves and become HT certified. If a portion of histologists don't recognize or care about the merit of certification how can we progress our profession to the status it deserves? You have to believe in yourself in order for others to follow suit. With the exclusion of the front office; NSH is a volunteer organization that fights for our profession and it can be difficult to take on the big dogs of ASCP/CAP/CLIA. I like to see this passion for our profession and if we keep this up eventually we will see results and the professional status we deserve. Jean Mitchell, BS HT (ASCP) University of Wisconsin Hospital Clinics Neuromuscular Laboratory 600 Highland Avenue Madison, WI 53792-5132 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Wednesday, September 11, 2013 8:30 AM To: 'Weems, Joyce K.'; 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT Histology does not get the respect or the recognition because histologists do not report results. All of the complex testing we do is overlooked because the pathologists report the results. CLIA standards are based on result reporting. The CAP has looked the other way for years because pathologists would hire unregistered techs. If pathologists would demand only registered techs half our battle would be won. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Wednesday, September 11, 2013 8:08 AM To: 'Jennifer MacDonald'; Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT And the reason so many have been fighting for this for years. If a lab were looking for a Medical Technologist there would be no question. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Tuesday, September 10, 2013 7:32 PM To: Marcum, Pamela A Cc: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] Unregistered HT As long as we do not need certification, licensure and minium education requirements we will not be recognized as Laboratory Professionals. From: Marcum, Pamela A pamar...@uams.edu To: 'joelle weaver' joellewea...@hotmail.com, 'Emily Sours' talulahg...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 09/10/2013 01:12 PM Subject:RE: [Histonet] Unregistered HT Sent by:histonet-boun...@lists.utsouthwestern.edu I agree we have huge gray areas and not all histology schools are as good as they could be for what we are facing in Histology. I keep harping on the fact that until we are recognized as Laboratory Professionals we will stay in this limbo. The rules determining complex testing should be revisited to what is done in Histology Laboratories today and not what we did 30 or more years ago. The Clinical Laboratory is now so automated it is hard to find anyone in most areas who can even remember doing any manual testing. The Micro lab is the closest to being as manual as areas of
[Histonet] RE: Processors and IHC Stainers
We like our 4 Ventana Ultras for IHC, ISH and CISH. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Wednesday, September 04, 2013 12:33 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Processors and IHC Stainers We are looking/hoping for some opinions on some equipment that we are considering for our lab. In the tissue processor line up we have: Leica's ASP6025; the VIP 6; Thermo's STP 420ES and Milestone's Logos. The IHC Stainer contenders are: Ventana's Ultra; Leica's Bond III or an updated version of what we currently use - the Labvision Autostainer. It's especially important to us to hear the opinions of techs who work with these instruments, as the selections here are being made primarily by those who do not. We are a community hospital with about 5800 cases/year. We average 25-30 IHC slides/day. Thanks in advance! Sharon Regional Health System is the area's largest hospital and provider of health care services. Visit us online at http://www.sharonregional.com for a complete listing of our services, primary care physicians and specialists, and satellite locations. Confidentiality Note: This message is intended for use only by the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: IHC Tissue
Hi Colleen, We are just wrestling with this very issue and have decided to use: Normal colon, i.e. staining present with all four antibodies Colon carcinoma without the mutation, i.e. staining present with all four antibodies Colon carcinoma(s) with the mutation for each antibody, i.e. no staining present for each of the four antibodies, probably employing two different specimens I'll be interested in your other responses since our plan is not yet set in stone. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Herring, Colleen Sent: Wednesday, August 28, 2013 6:14 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Tissue Good morning, I would like to get some input on the type of tissue everyone is using for their IHC MMR protocol. I would like to use just one block for all four protocols if possible. Any suggestions. The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone, and permanently delete the original e-mail, attachment(s), and any copies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Resulting Ventana HER2 dual ISH
Good morning everyone, For those if you that are using Ventana's Inform HER2 dual ISH DNA Probe Cocktail Assay; how are you reporting out results and do you put a disclaimer of any sort in your reports? Thanks for your responses, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Positive tissue controls for MSI antibody panel
Question everyone: What are you all using for your positive tissue control when running the MSI antibody panels, i.e. MLH-1, MSH-2, MSH-6, PMS-2? We're contemplating using normal colon that all four antibodies should stain but do we also need to show that colon having the mutation does not stain for each antibody as well to show there are no false positive results? The former would be the easy way out but I'm willing to construct a multi-tissue block containing normal colon and colon with the mutation(s). Thanks for all your responses, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p63 vs p40
Our docs here want to switch to TTF-1 and p40 for lung bxs rather than the TTF-1 and p63 we're doing now. We will retain our p63 for other applications. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Susan Foreman Sent: Thursday, August 15, 2013 8:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p63 vs p40 Interested in hearing feedback regarding p63 vs p40 antibodies for IHC. Our rep has provided me with some literature, but I wanted to hear some real-world feedback. J Many Thanks, Susan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: immunohistochemistry help please
To test your deparaffinization Rebecca, I'd try staining a slide with something cheaper and quicker, like just your counterstain to see if that's working. Then go from there. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tarantelli, Rebecca Anne Sent: Thursday, August 01, 2013 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] immunohistochemistry help please Hi all, I am new to this forum, but a friend recommended I send my issue out to you for help. Four weeks ago I completed a new protocol for Immunohistochemistry (IHC) using protinase K epitope retrieval, and monoclonal antibody 3F6. It worked perfectly. Three days later, after 13 beautiful stains, it just stopped working. The slides will not even take up the counter stain - Hematoxylin. I have exchanged my xylene, and tried again, and it still is not working. I am guessing since my counter stain isn't working, this is an issue in the deparaffinization stage, but I'm just not sure. Please let me know if you have any suggestions! Thank you Rebecca Rebecca Tarantelli ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: immunohistochemistry help please
OK, so I would be very suspect of your xylene. Has anything changed, i.e. vendor? It sounds like it's not dissolving your paraffin. Are you using the same paraffin you were when you were successful? Do you dry/melt your slides in an oven prior to depar? If not, you could try that at a temperature just above the melting point of your paraffin for 10 - 30 minutes. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: Tarantelli, Rebecca Anne [mailto:ra...@pitt.edu] Sent: Thursday, August 01, 2013 2:40 PM To: Sebree Linda A; histonet@lists.utsouthwestern.edu Subject: RE: immunohistochemistry help please Hi Linda, I'm sorry - I should have been a little more clear. Once I realized it isn't working, I did stop using the antibody. I have been trying just deparaffinization, and then counter staining with hematoxylin and skipping everything in between. The tissue isn't taking up the hematoxylin. With your experience, would you have any suggestions as to what part of the deparaffinization stage would cause the hematoxylin not to stain? I use: Xylene 3 minutes x 3, 100% ethanol 3 minutes x 2, 90 % ethanol 3 minutes x 1, 75% ethanol 3 minutes x1, distilled water 2 minutes x 1, then Dako wash buffer 3 minutes x 1. Next, I have been just going right to 3 minutes in my hematoxylin. I'm sorry if these are beginner questions, this is my first attempt at IHC, and it worked so well for a week and now nothing. Thank you! Rebecca Tarantelli -Original Message- From: Sebree Linda A [mailto:lseb...@uwhealth.org] Sent: Thursday, August 01, 2013 3:36 PM To: Tarantelli, Rebecca Anne; histonet@lists.utsouthwestern.edu Subject: RE: immunohistochemistry help please To test your deparaffinization Rebecca, I'd try staining a slide with something cheaper and quicker, like just your counterstain to see if that's working. Then go from there. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tarantelli, Rebecca Anne Sent: Thursday, August 01, 2013 2:20 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] immunohistochemistry help please Hi all, I am new to this forum, but a friend recommended I send my issue out to you for help. Four weeks ago I completed a new protocol for Immunohistochemistry (IHC) using protinase K epitope retrieval, and monoclonal antibody 3F6. It worked perfectly. Three days later, after 13 beautiful stains, it just stopped working. The slides will not even take up the counter stain - Hematoxylin. I have exchanged my xylene, and tried again, and it still is not working. I am guessing since my counter stain isn't working, this is an issue in the deparaffinization stage, but I'm just not sure. Please let me know if you have any suggestions! Thank you Rebecca Rebecca Tarantelli ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Hepatocyte Nuclear Factor (HNF-1B)
Good morning all, One of our pathologists has requested this antibody and we don't have it in our inventory. So I'm asking if there are any reference labs offering HNF-1B for FFPE human tissue? Thanks for any/all responses. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Sub-optimal Results
We are required to keep all QA forms for 2 years. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie Sent: Tuesday, July 16, 2013 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sub-optimal Results Do you guys keep the QA form when a specimen is rejected and sent back to the source for correction? ANP. 11475 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] MSI on Ventana
Hey Tony, We are using VMS antibodies (2 are manufactured by CellMarque) on our Ultras. We've had to go with the Optiview detection to get satisfactory results. Let me know if you'd like our protocols. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tony Reilly Sent: Monday, July 08, 2013 9:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MSI on Ventana Hello We have previously been sending our MSI Abs to another lab where they are being performed on the Bond. We have now been informed that we are to do our own and one of my IHC staff with previous experience in another lab informs me that they do not work as well on the Ventana platforms that we are currently running. Is ther anybody out there successfully staining MSI on the Ventana. If so what brand/clone of Abs are you using? We are trying to avoid using the Ventana RTU Abs due to cost. regards Tony Tony Reilly B.App.Sc. , M.Sc. Chief Scientist, Anatomical Pathology Pathology Queensland-PA Laboratory Health Services Support Agency | Department of Health Level 1, Building 15,Princess Alexandra Hospital Ipswich Road,WOOLLOONGABBA Qld4102 Ph: 07 3176 2412 Mob: 0402 139411 Fax: 07 3176 2930 Email: tony_rei...@health.qld.gov.au Web: www.health.qld.gov.au/qhcss/ This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. ** ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Immunohistochemistry TAT
Pat, Right off the bat I can see 2 things that could speed things up for you. We have Ultras and only dry our slides for 10 minutes at 60 d C. If tissues don't stay on well, try some Stay On in your water bath; we're experimenting with that now. Also, we spend way too much time ourselves searching for blocks. See if there is a way to have them organized immediately after sectioning the HEs so they are quicker to find...we are struggling with this. My 2 cents; good luck. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Karlisch, Patricia Sent: Monday, July 01, 2013 4:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Immunohistochemistry TAT Histonetters, I am looking for some help in getting IHC slides out in an 8 hour period of time. Here is some information. * We have a busy Immunohistochemistry lab and the volume of requests keep increasing. We offer approximately 150 different IHC stains ( including ISH and Dual ISH for Her 2). Generally, there are request for 150- 200 IHC tests twice a day, not including negative and positive (same slide) controls. * The techs pull an AM log at 6am and another at 1pm. The 1pm log will be handled while waiting for the IHC's to be completed from the 6am run and these will always be run overnight. * The 6am log requires that one tech start cutting each block for multiple IHC stains. (A search for blocks and the necessary control slide occurs by the same person). The slides are kept in a 60 degree oven for about 60 minutes. Most of these slides will be ready by 2-3:30pm. Here is my question. If we allowed requests for IHC stains to wait until 9AM how could we still get slides out by 3:30pm-4pm? In other words we would not have access to an LIS log until 9am so that the pathologists could order from the morning biopsies. What is the best way to do this with 3 Ventana Ultra's and one Benchmark XT. The blocks ( usually 40-50) need to be cut; sit in an oven and then get labeled and placed on the Ventana's. All longer stains such as ISH would be run overnight. The following tasks makes the 3:30- 4pm deadline difficult: * Searching for the blocks * Cutting the blocks (40-50) onto control slides + negative control * One hour in the oven to dry (Can we use 30minutes?) * Attaching labels to 150 slides and setting up instruments * One microtome * 2 techs/ some of the time Can you share your workflow with me if you do a similar volume of slides within an 8 hours period. How would you staff the two IHC techs to gain the most efficiency. As you know the fuller the instrument the longer the staining process. Thank you, Pat Karlisch, Supervisor pkarli...@hmc.psu.edumailto:pkarli...@hmc.psu.edu Tel 717-531-6072 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Elastin stain
Do any of you do this? Do any referral labs offer this? Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC antibody for Major Basic Protein for eosinophils
Are there any reference labs offering this on FFPE specimens? Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Lysol IC for Ventana equipmetn cleaner
The dilution is listed on the bottle...I'm not at work but its something like 1:252. Linda A. Sebree From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Amber McKenzie [amber.mcken...@gastrodocs.net] Sent: Tuesday, May 14, 2013 11:33 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells Lysol IC in its place and I was wondering how you guys make it up...water to cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl. Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] H pylori and Ventana instruments
Rae, We use Ventana's HP antibody with no problems. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rae Staskiewicz Sent: Tuesday, May 07, 2013 12:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H pylori and Ventana instruments Hi all, Those of you using Ventana instrumentation, could you tell me what H pylori antibody you are using, and whether or not you are experiencing non-specific staining, or staining that looks specific but with a stippled effect. Thanks Rae Staskiewicz UnityPoint Health-Methodist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Monitoring of IHC staining trends
Ashley, Every new lot of antibody we get in is run with some of our control tissues and compared to the current/previous lot staining. So we should be able to detect a change. Our policy is that minor changes to protocols are OK but anything major would require revalidation...we try not to go there. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Troutman, Kenneth A Sent: Tuesday, April 30, 2013 12:28 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Monitoring of IHC staining trends Hello Histonet, I have a question for the group at large. How are labs monitoring drift in IHC staining over time? Here's the scenario: You do lot to lot testing and everything looks fine until one day your pathologists are telling you that the CAM5.2 is too dark. Now, you've been looking at these slides every day for the last year and, sure enough, when you pull out a slide from last year's lot, it is significantly lighter. So what do we do about it? Do we revalidate the stain? Does anyone have a mechanism to monitor this better? What is the threshold for revalidation? Feedback from techs as well as any pathologists would be greatly appreciated. Regards, Ashley Troutman BS, HT(ASCP) QIHC Immunohistochemistry Supervisor Vanderbilt University Histopathology 1301 Medical Center Drive TVC 4531 Nashville, TN 37232 ashley.trout...@vanderbilt.edumailto:ashley.trout...@vanderbilt.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: ventana counterstaining
Kimmie, We use VMS' Hematoxylin II with no changes from how it has always stained. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimmie Rabe Sent: Thursday, March 21, 2013 11:28 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] ventana counterstaining Has anyone else had problems with the counterstain on the ventana being pink instead of that lovely pale blue color? Ventana says it's because we use Sta-On, but we don't always use Sta-On and the pink vs. blue staining doesn't correlate with whether Sta-On has been used or not. Don't get me wrong, I have nothing against pink. But if there is some problem with the hematoxylin we purchase from ventana or the dispensing or mixing thereof--we would like to know. Thanks! Kimmie E. Rabe, MD North Central Pathology, PA 3701 12th Street North, Suite 201 St. Cloud, MN 56303 Phone: 320-253-6554 Fax: 320-253-1218 k...@ncpath.commailto:k...@ncpath.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Glutamine Synthetase and HSP 70 IHC test availability
Hello Histonetters, One of our pathologists is requesting Glutamine Synthetase and Heat Shock Protein (HSP) 70 immunostains on a liver specimen. Our usual go to reference labs do not perform these. Are there labs out there that have these tests available? Even non-reference labs??? Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Ubiquitin and alpha synuclein
Jennifer, We use p62/SQSTM1 from MBL (Medical Biological Laboratories, Co., LTD), code M162-3, as our Ubiquitin and alpha Synuclein from Invitrogen, #18-0215. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harvey, Jennifer Lynn Sent: Thursday, December 13, 2012 11:03 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Ubiquitin and alpha synuclein What is histoland using to stain Lewy bodies these days? I am not happy with the ubiquitin or the alpha synuclein that we have. I would like to know what manufactures others are using. Thanks Jennifer Harvey, HT(ASCP) QIHC Vanderbilt University Medical Center Neuropathology Lab Supervisor C-2309 Medical Center North Nashville, TN 37232-2561 Phone: 615-343-0083 Fax: 615-343-7089 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Any reference labs offering CD52 (CAMPATH) on human FFPE specimens?
Looking for a reference lab offering the above. Thanks, Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Background staining on H pylori
Deloris, We recently had what we think was a contaminated dispenser of HP from VMS. It had brown particulate matter scattered over the slide which the docs said was not bugs. Received a new dispenser from VMS and have had no problem since. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Monday, November 05, 2012 5:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Background staining on H pylori Hi, I'm getting a lot of background staining on my HP's. We use a Ventana BenchmarkXT with ultraView DAB. The problem seems to be escalating of late, and I'm not sure why. We use Hollande's on our GI biopsies, and run them on a shorter run. Nothing has changed in the processing of the specimens, or the IHC procedure. The antibody dispenser is a newer lot, but not brand new, as we get the larger size due to the high volume of HP's we run. Any ideas? Deloris Carter HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How to fix frozen section?
It depends upon what marker you're staining for. We fix our renal biopsies for C4d in acetone for 10. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Tuesday, November 06, 2012 1:41 PM To: histonet Subject: [Histonet] How to fix frozen section? Hello, I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me? Thanks, Amy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC negative controls
I really doesn't matter what I think of course Melissa. When I presented the facts to the faculty they overwhelmingly agreed not to drop the negative controls and they were definitely not going to be in direct conflict with CLIA reg's. It's really up to your lab's manager/director whether an extensive validation is adequate. Good luck with your decision, Linda -Original Message- From: Kuhnla, Melissa [mailto:melissa.kuh...@chsli.org] Sent: Monday, October 29, 2012 5:23 AM To: Sebree Linda A; Glen Dawson; histonet Subject: RE: [Histonet] IHC negative controls Very good point Linda. The only testing we currently report as an FDA approved test are our breast markers. It may be easy enough to maintain the negative in that scenario. For other scenarios, we are considering dropping the negative as long as we perform and document an extensive validation. It still may be well worth it. What do you think? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, October 26, 2012 10:24 AM To: 'Glen Dawson'; histonet Subject: RE: [Histonet] IHC negative controls Hey Glen, Don't know the answer to your specific question but on a related issue, our lab has decided to maintain running negative controls due to the fact that the manufacturer's data sheets for our IHC detection kits and a majority of our antibodies require using +/- controls. The company in question has told us they do not intend to change their guidelines as its part of the FDA approval for their products. Performing IHC staining not in compliance with the manufacturers' guidelines is against CLIA reg's. Something to consider. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Friday, October 26, 2012 8:45 AM To: histonet Subject: [Histonet] IHC negative controls All, Can anyone tell me if JHACO CLIA are deferring to CAP's judgement that a negative control is not needed when utilizing a polymer detection? I assume that this is the case, but I'd like to be sure. Thank-you in advance, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Janesville, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Dako vs Ventana IHC systems
We sure like it Joe. -Original Message- From: Joe W. Walker, Jr. [mailto:joewal...@rrmc.org] Sent: Monday, October 29, 2012 9:38 AM To: Terri Brown; Sebree Linda A; Burton, Lynn; Gloria Tharp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako vs Ventana IHC systems Which Dako and Ventana machines are you all using? We are in the market for a new IHC stainer and the new Ventana BenckMark Ultra machine looks interesting. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewal...@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Terri Brown Sent: Monday, October 29, 2012 10:26 AM To: Sebree Linda A; Burton, Lynn; Gloria Tharp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako vs Ventana IHC systems We had Ventana and changed to Dako. The Dako phone tech support is excellent and we never wait long for in house service. Our pathologists love the turnaround time with the open system and the stain quality. Terri H. Brown, HT (ASCP) Pathology Laboratory Manager Northside Hospital Atlanta terri.br...@northside.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Friday, October 26, 2012 4:52 PM To: 'Burton, Lynn'; Gloria Tharp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako vs Ventana IHC systems I concur with Lynn; we've been dealing with Ventana 19 years. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Friday, October 26, 2012 3:47 PM To: Gloria Tharp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako vs Ventana IHC systems We have been dealing with Ventana for at least 10 years with very good service and reliability. Their phone tech support is excellent and we have never waited long for in house service. Lynn Burton Lab Associate Animal Disease Lab Galesburg, Il -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp Sent: Friday, October 26, 2012 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako vs Ventana IHC systems Does anyone have info about Dako vs Ventana IHC systems as to consistency and reliability as far as turn around time on tech repair on site. Thanks. Gloria Tharp, BA, HTL(ASCP) Director of Operations PCA Southeast gth...@pcasoutheast.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu
RE: [Histonet] Dako vs Ventana IHC systems
Joe, You can still use your Dako antibodies (we have several) with the Ultra; or anyone else's antibodies for that matter. I don't work for VMS, in case you're wondering, we've just had a very reliable and good relationship with them for a long time and we've always been happy with their immunostainers. Linda Sebree -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Monday, October 29, 2012 2:55 PM To: CHRISTIE GOWAN; terri.br...@northside.com; Sebree Linda A; lynn.bur...@illinois.gov; gth...@pcasoutheast.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako vs Ventana IHC systems Thank you for this information, Christie. We are a much smaller lab and would be using the new system as our primary IHC stainer. Workflow considerations are a primary concern for us in addition to staining consistency and ease of use. From what was demonstrated to us, the Ultra did not appear to be that closed and provided lots of flexibility for running just about anything we wanted but it was a sales pitch. We are still in the researching our options stage. We have experienced less than desirable service from Dako but they currently have a presence in our lab. We have been pretty happy with the antibodies from Dako. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewal...@rrmc.orgmailto:joewal...@rrmc.org www.rrmc.orghttp://www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence From: CHRISTIE GOWAN [mailto:christiego...@msn.com] Sent: Monday, October 29, 2012 12:15 PM To: Joe W. Walker, Jr.; terri.br...@northside.com; lseb...@uwhealth.org; lynn.bur...@illinois.gov; gth...@pcasoutheast.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako vs Ventana IHC systems Hi Joe, When searching for an automated IHC stainer, you need to shop based on your facility and it's needs. We recently went through the whole shopping around experience and because we are such a large facility, we have the Ventana Ultra as well as an XT and the New Dako Link. We use the Ventana Ultra for single piece flow and it really changed the dynamic of our lab. We also use the Ventana for all our automated probes including the dual Her2/Chr17 CISH. The Dako's (we have 2) we use for all those things that we do not run on the Ultra or Benchmark XT. About 50/50 on each system. We also do a lot of research for the UAB campus and it is nice to have the more open DAKO systems. We have 3 very experienced IHC techs working in the lab which lends a lot of consistency to the staining. I say this because if you rotate people through the lab or your staff is less experienced then you want something that is easy and consistent. We will probably always have the Ventana systems in our lab so not much shopping around for a more or less closed system (there is always debate over the open/closed thing) However, shopping around for a more open system can be a little harder. We demo'ed the bond, Biocare, Dako and Labvision. We narrowed it down to Biocare and Dako based on ease of use, speed and capacity and reagents/antibodies. Once we had narrowed it down to the 2 companies, then we started looking at packages. Dako delivered the best package and because Dako already had a presence in our Histology lab (Artisan Special Stainer) and we knew their service was always prompt (we always struggled with this with Leica) We chose Dako and replaced our 2 older Labvisions with Autostainer Links. If you have any questions you can let me know via email and I will give you our contact info. Best of luck to you. Christie Gowan UAB Hospital Birmingham, AL From: joewal...@rrmc.orgmailto:joewal...@rrmc.org To: terri.br...@northside.commailto:terri.br...@northside.com; lseb...@uwhealth.orgmailto:lseb...@uwhealth.org; lynn.bur...@illinois.govmailto:lynn.bur...@illinois.gov; gth...@pcasoutheast.commailto:gth...@pcasoutheast.com; histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern .edu Date: Mon, 29 Oct 2012 14:38:20 + Subject: RE: [Histonet] Dako vs Ventana IHC systems CC: Which Dako and Ventana machines are you all using? We are in the market for a new IHC stainer and the new Ventana BenckMark Ultra machine looks interesting. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewal...@rrmc.orgmailto:joewal...@rrmc.org www.rrmc.orghttp://www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional
RE: [Histonet] IHC negative controls
Hey Glen, Don't know the answer to your specific question but on a related issue, our lab has decided to maintain running negative controls due to the fact that the manufacturer's data sheets for our IHC detection kits and a majority of our antibodies require using +/- controls. The company in question has told us they do not intend to change their guidelines as its part of the FDA approval for their products. Performing IHC staining not in compliance with the manufacturers' guidelines is against CLIA reg's. Something to consider. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Glen Dawson Sent: Friday, October 26, 2012 8:45 AM To: histonet Subject: [Histonet] IHC negative controls All, Can anyone tell me if JHACO CLIA are deferring to CAP's judgement that a negative control is not needed when utilizing a polymer detection? I assume that this is the case, but I'd like to be sure. Thank-you in advance, Glen Dawson BS, HT(ASCP), QIHC Histology Technical Specialist Janesville, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Dako vs Ventana IHC systems
I concur with Lynn; we've been dealing with Ventana 19 years. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn Sent: Friday, October 26, 2012 3:47 PM To: Gloria Tharp; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako vs Ventana IHC systems We have been dealing with Ventana for at least 10 years with very good service and reliability. Their phone tech support is excellent and we have never waited long for in house service. Lynn Burton Lab Associate Animal Disease Lab Galesburg, Il -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp Sent: Friday, October 26, 2012 2:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako vs Ventana IHC systems Does anyone have info about Dako vs Ventana IHC systems as to consistency and reliability as far as turn around time on tech repair on site. Thanks. Gloria Tharp, BA, HTL(ASCP) Director of Operations PCA Southeast gth...@pcasoutheast.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] validation runs
In our opinion, yes. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Melissa Whitaker Sent: Tuesday, October 23, 2012 9:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] validation runs Hi everyone in IHC, are controls needed when performing antibody validation runs? Thanks all! -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Stain for HP
H. Pylori by IHC; automated. I believe they're pretty much routinely ordered; sure seems like it anyway! Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, October 17, 2012 10:04 AM To: Histonet Subject: [Histonet] Stain for HP What stain are you using for HP?--Giemsa. Warthin Starry or IHC. Do you do them routinely or only when requested? Are they done on an autostainer or with a kit? Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] duel stains
We've always just billed for one stain so no difference for us; we'll continue doing it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of llhartma...@comcast.net Sent: Monday, October 01, 2012 6:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] duel stains Hi Netters, Just curioushas anyone stopped doing dual IHC (ie. PIN4) du e to the charging changes? We are going to start doing the three stains separately. Any opiniions? Linda Hartman HT(ASCP) MNMC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC new reagent lots
We run a control(s) first before using it on patient samples. We compare that slide to the same/similar control(s) run with the current lot. Our director reviews both slide(s) and if comparable or better, signs off on the new lot. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter Sent: Monday, October 01, 2012 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC new reagent lots I'm in a new position and have to get our IHC paperwork CAP ready. I'm not sure about something. When you put a new antibody dispenser (with a different lot # than previously used) on the IHC stainer, what QC needs to be done? Can the control run with a patient test serve as your QC, or does it need to be a separate slide done before you run any patient tests with that new lot #? Also, I know that CAP is no longer requiring negative controls to be run when using a biotin free detection system. Can we just stop running the negative controls, and reference the CAP change in our protocol, or do we have to perform some sort of validation? I appreciate any help. Deloris ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Changing from Ventana IView Detection Kit toVentana Ultraview kit
Joe, We are abiding by the CAP 10/10 guidelines when at all possible and then we compare our results with another method or same method but other lab, i.e. reference or another clinical lab willing to trade slides with us. The comparison part is where we are having issues as we, not me personally, don't want to pay a reference lab for the comparison work so we rely on others in our Histonet family willing to run our slides. And of course, we're squeezing as many cases on a single slide as possible. As to your question about 5/5 or more, CAP leaves it up to each lab as to whether its feasible and possible to obtain their recommended quota. Interesting thread as my days are spent in the middle of this exercise. Linda Sebree -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, September 25, 2012 5:35 PM To: 'Vanessa Perez'; 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection Kit toVentana Ultraview kit We are having a lively discussion about having 10 known positives and 10 known negatives to validate new antibodies. Many years ago we set up 5 and 5 even before CAP thought of the idea. This year's checklist added the 10 and 10 part, but it is up to the medical director. What is everyone else doing out there? We are using the Ventana UltraView detection kits. Everyone who uses these kits know how expensive they are. Is 5 and 5 sufficient or should go by CAP recommendations? Joe Nocito -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Tuesday, September 25, 2012 2:37 PM To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit As far as lot to lot validation that's all we do. Use same control and compare both. Now validating a new detection kit is a whole different story. Here I just made a checklist of all the antibodies we do and had the doc sign off on each stain with the new kit. If you want you can do a slide of each with same control one with the iview and one with the ultraview. All depends on how your doc wants to validate it. Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 25, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit We are trying to decide how to validate our stains when we switch from Ventana's IView kit to their Ultraview Kit. I have reviewed the CAP question on this and find the following wording: The performance of new lots of antibody and detection system reagents are compared with old lots before or concurrently with being placed into service. Note: Parallel staining is required to control for variables such as disparity in the lots of detection reagents or instrument function. New lots of primary and detection reagents must be compared to the previous lot using an appropriate panel of control tissues. This comparison must be made on slides cut from the same control block. Evidence: Written procedure and records of verification of new reagent lots. For new lots of antibodies we have been running the new lot and comparing with the previous lot by reviewing the control slide from the old lot to the new lot. Is this sufficient? Wording that bothers me is appropriate panel of tissues Thanks for your input. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Changing from Ventana IView Detection KittoVentana Ultraview kit
We made our detection kit change when we purchased new Ultra immunostainers so of course validation covered many bases. If we were just changing detections, we'd run a couple positive controls for each antibody with the new detection and compare them to the same controls run with our current detection. If the results are comparable OR BETTER, our director would OK them. If not, we'd tweak that antibody for the new detection until it passed. A similar procedure is followed for new lots of detection but we limit it to one control and one antibody: we've never had discrepant comparison results. Linda -Original Message- From: Kuhnla, Melissa [mailto:melissa.kuh...@chsli.org] Sent: Wednesday, September 26, 2012 9:15 AM To: Sebree Linda A; Joe Nocito; Vanessa Perez; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection KittoVentana Ultraview kit Yes I completely agree that this is up to each medical director. This detection kit change is considered a major change but keep in mind they are similar products from the same vendor. Changing your detection is a very universal step. Think of your validation in a more universal way. 10/10 is extremely time consuming and expensive. For some antibodies, it will take years to accumulate ten positive cases. You could run some common panels of antibodies you see often. You could select some stains that are for cytoplasm, nuclear, membrane staining. You should pay more attention to any prognostic marker (ER/PR, CD20, ckit). Just some ideas. Best of luck. Melissa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, September 26, 2012 8:43 AM To: Joe Nocito; Vanessa Perez; Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection KittoVentana Ultraview kit Joe, We are abiding by the CAP 10/10 guidelines when at all possible and then we compare our results with another method or same method but other lab, i.e. reference or another clinical lab willing to trade slides with us. The comparison part is where we are having issues as we, not me personally, don't want to pay a reference lab for the comparison work so we rely on others in our Histonet family willing to run our slides. And of course, we're squeezing as many cases on a single slide as possible. As to your question about 5/5 or more, CAP leaves it up to each lab as to whether its feasible and possible to obtain their recommended quota. Interesting thread as my days are spent in the middle of this exercise. Linda Sebree -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, September 25, 2012 5:35 PM To: 'Vanessa Perez'; 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection Kit toVentana Ultraview kit We are having a lively discussion about having 10 known positives and 10 known negatives to validate new antibodies. Many years ago we set up 5 and 5 even before CAP thought of the idea. This year's checklist added the 10 and 10 part, but it is up to the medical director. What is everyone else doing out there? We are using the Ventana UltraView detection kits. Everyone who uses these kits know how expensive they are. Is 5 and 5 sufficient or should go by CAP recommendations? Joe Nocito -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Tuesday, September 25, 2012 2:37 PM To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit As far as lot to lot validation that's all we do. Use same control and compare both. Now validating a new detection kit is a whole different story. Here I just made a checklist of all the antibodies we do and had the doc sign off on each stain with the new kit. If you want you can do a slide of each with same control one with the iview and one with the ultraview. All depends on how your doc wants to validate it. Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 25, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit We are trying to decide how to validate our stains when we switch from Ventana's IView kit to their Ultraview Kit. I have reviewed the CAP question on this and find the following wording: The performance of new lots of antibody and detection system reagents are compared with old lots before or concurrently with being placed into service
RE: [Histonet] negative controls
I asked my VMS rep that question and she forwarded it to the higher ups as they had not heard about this yet. Waiting for an answer but even if VMS says we can omit them, our negative aren't all that clean always so don't know if we will or not. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of anita dudley Sent: Wednesday, August 01, 2012 4:13 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative controls just wondering if we can start leaving the negative controls off of our immuno stains now as long as our procedure says we can stain without them? we use ventanas multimer detection systems. thanks so much, anita dudley providence hospital mobile, alabama ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] A poem
I LOVE this Bernice! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Thursday, June 28, 2012 11:15 AM To: Fellow HistoNetters Subject: [Histonet] A poem I've had this for years- don't know where I got it but thought you all might be amused. LAB HIERARCHY Chief Pathologist Leaps tall building in a single bound Is more powerful than a locomotive Is faster than a speeding bullet Walks on water, Gives policy to God Associate Pathologist Leaps short buildings in single bound Is more powerful than a switch engine Is just as fast as a speeding bullet Walks on water if sea is calm Talks with God The Department Head Leaps short buildings with a running start and favorable winds Is almost as powerful as a switch engine Is faster than a speeding bullet Walks on water in an indoor pool Talks with God if special request is approved Director of Laboratories Rarely clears a Quonset hut Loses tug of war with locomotive Can fire a speeding bullet Swims well Is occasionally addressed by God Associate Director of Laboratories Makes high marks on the walls when trying to leap tall buildings Is run over by locomotives Can sometimes handle a gun without inflicting self-injury Dog paddles Talks to animals Supervisor Runs into building Recognizes locomotives two out of three times Is not issued ammunition Can stay afloat with a life jacket Talks to walls Chief Technologist Falls over doorstep when trying to enter building Says look at the choo-choo Wets themselves with a water pistol Plays in mud puddles Mumbles to Themselves Histotechnologist Lifts tall buildings and walks under them Kicks locomotives off the tracks Catches speeding bullets in their teeth and eats them Freezes water with a single glance They are God - Anonymous Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility ECOGPCO-RL Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] looking p16 - where is mtm labs?
As far as I know you may only purchase MTM p16 through Ventana now...FDA approved. BD sells p16 buts it's RUO. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Settembre, Dana Sent: Tuesday, June 12, 2012 11:06 AM To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: [Histonet] looking p16 - where is mtm labs? Looking to get current pricing and availability from mtm labs on p16 and no one answers their phone - 800# nor their direct# Seems Roche has taken them over. Need to reach them. Any help would be appreciated Dana Settembre Immunohistochemistry University Hospital - UMDNJ Newark, NJ USA Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ANTIBODY VALIDATION PROCEDURE
Follow the CAP guidelines for antibody validation; that's what we do. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wilson A Sent: Wednesday, May 16, 2012 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ANTIBODY VALIDATION PROCEDURE Hi, How are you guys doing? I hope you are doing great. Please I will appreciate it, if you guys have a procedure on the ANTIBODY VALIDATION and would like to share it with me. Thanks Wilson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Immunofluorescence in the clinical laboratory
Yes as well as the tissue usually run with these antibodies. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Monday, May 14, 2012 10:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Immunofluorescence in the clinical laboratory To those doing DIF in the clinical lab, are you using frozen tonsils or other tissue as control material when you do these? Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fwd: Plants
I've always had at least one; makes the day more tolerable. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz Sohrab Sent: Friday, May 11, 2012 12:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fwd: Plants I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? Thank you, Behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Fwd: Plants
Yeah but they used to use canaries in mines to warn of toxic levels of gases; having one in a histo lab might be a VERY good idea! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William Sent: Friday, May 11, 2012 12:33 PM To: Behnaz Sohrab Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Fwd: Plants I have had plants in a number of labs. Could be against the rules, but I never saw it. I even had a canary in one lab - pretty sure that is against the rules. Will Chappell Sent from my iPhone On May 11, 2012, at 1:29 PM, Behnaz Sohrab sohra...@ah.org wrote: I was told by infectious control person that plants are not allowed in the lab?? IS this true? any experience with this? Thank you, Behnaz ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IMF antibody validation
Hello everyone, I'm branching out into IMF antibodies and as such am starting to validate these antibodies. Previously no comparison studies were done that I know of. Now we're running frozen tissue slides manually with our current set of antibodies simultaneously with new vendors' antibodies being run automated. Since IMF is not permanent, my question to all of you is, are you photographing the slides for a permanent record or just keeping a written record of the comparison results? Any and all responses are welcome. Thanks, Linda Sebree ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reference lab needed for Alzheimer antibodies
Good morning Histonetters, For purposes of validation, I'm looking for a reference lab that performs IHC on human FFPE samples for several Alzheimer antibodies. Specifically these antibodies are: PHF-1, CP13, anti-TDP-43 and LB 509 10. I'd also be interested in any private/hospital/research labs performing these. Thanks, Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] New Reagent Lot Verification
We're saving both for at least 2 years. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Thursday, April 12, 2012 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] New Reagent Lot Verification ANP.22760 refers to new lot verification for antibodies and detection. The Evidence of Compliance says that there should be Records of verification of new reagent lots. What is everyone's interpretation of records? Are you saving slides in addition to paper documentation? CONFIDENTIALITY NOTICE This message and any included attachments are from Somerset Medical Center and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or trade secret information entitled to protection and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call Somerset Medical Center's computer Help Desk at 908-685-2200, ext. 4050. Be sure to visit Somerset Medical Center's Web site - www.somersetmedicalcenter.com - for the most up-to-date news, event listings, health information and more. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Slippery Floor due to paraffin
Peel off film here...works well. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Wednesday, April 04, 2012 11:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slippery Floor due to paraffin Hello to all in histoland. What are histology labs doing to combat the slipperiness of the floor due to paraffin. Are you using rugs, peel away films ? Any help would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reference lab doing West Nile Virus and HHV6 IHC on human FFPE specimens
Yup, that's what I'm looking for Histonetters. Any info. is appreciated. Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana XT/Ultra
Ventana can give you these numbers based on your contract pricing for bulk fluids. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Wednesday, March 28, 2012 10:43 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Ventana XT/Ultra Has anyone ever worked up cost/slide for either the XT and/or Ultra as far as the bulk fluids are concerned? Does anyone know how much bulk fluid (LCS, Reaction buffer, CC1) is used per slide during a typical run? thanks! Clare Clare J. Thornton, HTL(ASCP), QIHC Assistant Histology Supervisor Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Reference lab doing GLG-1 (MG-160)?
Does anyone know of a reference lab offering this antibody for aggressive pituitary tumors in FFPE human tissue? Thanks, Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC + control question
Vimentin -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chiriboga, Luis Sent: Tuesday, March 06, 2012 8:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC + control question Posting for a colleague: Which of the following choices Cytokeratin (NOS) Vimentin s-100 Cd45 Desmin Would be the best to use as an internal positive control for fixation and processing? Any supporting literature/references/docuemntation would be very helpful... Thanks Luis ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] new antibody validation
We have been picking (or trying to) 10 positive cases that have negative tissue elements included. Obviously for some hard-to-find positive cases we need to make due with what's available. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Bryant Sent: Thursday, March 01, 2012 9:56 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] new antibody validation ANP.22750 The laboratory has documented validation of new antibodies, prior to use in patient diagnosis. In the CAP's note it states that a panel of 10 positive and 10 negative neoplasms would be sufficient for a well-characterized antibody. How extensive a panel of tissues are laboratories using to meet this checklist requirement? Is it necessary to do 10 positive and 10 negatives or can it be at the discretion of the laboratory director? Carol Bryant, CT (ASCP) Cytology/Histology Manager Lexington Clinic Phone (859) 258-4082 Fax (859) 258-4081 cb...@lexclin.com NOTICE OF CONFIDENTIALITY This message, including any attachments, is intended only for the sole use of the addressee and may contain confidential or privileged information that is protected by the State of Kentucky and/or Federal regulations. If you are not the intended recipient, do not read, copy, retain or disseminate this message or any attachment. If you have received this message in error, please call the sender immediately at (859)258-4000 and delete all copies of this message and any attachment. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. Neither the transmission of this message or any attachment, nor any error in transmission or misdelivery shall constitute waiver of any applicable legal privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Ventana xt
Side note: only the Ventana Ultra allows adding slides as you go, not the XT. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Friday, February 17, 2012 7:52 AM To: Christie Gowan; dphill...@vetmed.lsu.edu; histonet Subject: RE: [Histonet] Ventana xt I think the Ventana XT is the best instrument for adding slides as you go which makes it perfect for a LEAN lab. This will reduce your TAT.The instrument is so easy to use it is virtually impossible to mess up with it's bar coding and visual control. Very user friendly. Jan Mahoney,Omaha, NE From: christiego...@msn.com To: dphill...@vetmed.lsu.edu; histonet@lists.utsouthwestern.edu Date: Thu, 16 Feb 2012 19:46:35 + Subject: RE: [Histonet] Ventana xt CC: Pros: Lends itself well to labs that do basic IHC staining Antibodies are pre-diluted so no guess work on dilutions Good for batching runs Consistent quality of slides 24 hour technical support Can run molecular probes protocols are easy to adjust Cons: Closed system Must use Ventana antibodies or purchase special dispensers if using non Ventana products Pre-dilute antibodies are pricey Stand alone instrument so must have space for it I'm sure there is more but you just really need to see what your needs are. We have the XT and Ultra as well as open systems. We love the Ventana's but we also will always have open platforms because we do a lot of research. The work flow is another thing you need to look at. How many slides do you turn out in one day? What is your turn around time? The XT is a great instrument but it depends on your lab. Hope this helps. Christie Gowan UAB Hospital From: dphill...@vetmed.lsu.edu To: histonet@lists.utsouthwestern.edu Date: Thu, 16 Feb 2012 12:29:37 -0600 Subject: [Histonet] Ventana xt Looking for any pros and cons on the Ventana XT. Thanks Del ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] slide file storage to dry slides
Good morning all, We've recently switched from film coverslipping back to glass and therefore need to thoroughly dry our slides before permanent filing. I recall, in my first histology job30 + years ago, that we used metal stacking slide files that you could put an insert into the drawers that looked like a non-stretchy spring. The wires of this spring held the slides apart to dry, then they could be filed without the spring when they were completely dry. Anyone know if that product still exists? Or does anyone have a better solution for drying slides while still keeping them in order? Thanks for the assist, Linda ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] H. Pylori - IHC
We use Ventana's, manufactured by Cell Marque. Most, if not all, of out pathologists have opted for this instead of histochemical staining for HP. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of beth@hcahealthcare.com Sent: Wednesday, November 16, 2011 4:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H. Pylori - IHC Does anyone have recommendations for a good vendor for H.Pylori for IHC? We have tried many, but our pathologists complain that they are not specific for H.Pylori and that all gram negative bacteria are staining. Currently we are not running this but our pathologists are interested in it. Thanks! Beth A. Fye, CT (ASCP) Pathology Technical Manager HCA Richmond Hospital Laboratories office: (804)228-6564 fax: (804)323-8638 mailto:beth@hcahealthcare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
