[Histonet] (no subject)

[Rhonda McCormick via Histonet]

Hi there, 
Any recommendations for a ductless, tabletop, fume hood (roughly 27 wide x 22 
deep x 13 high)? I have done searches online, but would appreciate any 
recommendations.
Thank you!Rhonda McCormick
Medical laboratory supervisor

CVM Histology Diagnostic Laboratory,

Texas A University

College station-TX-77843, TAMU -4467


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[Histonet] (no subject)

[Lauri Zaker via Histonet]

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Re: [Histonet] (no subject)

[Tony Reilly via Histonet]

Hello Rhonda
I have extensive experience with the Brown Hopps stain.

I would advise against only staining for gram negative as both the gram 
positive stain and the gram negative stain will stain both gram positive and 
gram negative organisms.  The differentiation is only achieved by the 
destaining steps.

If you only used the gram negative steps you would still be staining both gram 
positive and gram negative organisms negatively.

If you you want more information let me know .

Regards
Tony Reilly
Histologist
Australia



Sent from my iPhone

> On 4 Jul 2023, at 5:30 am, Mac Donald, Jennifer via Histonet 
>  wrote:
> We have good success with the Twort.
> 
> -Original Message-
> From: Rhonda McCormick via Histonet 
> Sent: Monday, July 3, 2023 11:22 AM
> To: Histonet 
> Subject: [Histonet] (no subject)
> 
>  EXTERNAL SENDER - Exercise caution with requests, links, and attachments.
> 
> Hello, I'm wondering if anyone does the Brown-Hopps Gram stain for gram 
> negative bacteria. If so, would you mind to send your protocol please?
> Any recommendations for a gram negative (only) stain - specifically E. coli? 
> One of our residents is asking.
> Thank you!
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Re: [Histonet] (no subject)

[Mac Donald, Jennifer via Histonet]

We have good success with the Twort.

-Original Message-
From: Rhonda McCormick via Histonet 
Sent: Monday, July 3, 2023 11:22 AM
To: Histonet 
Subject: [Histonet] (no subject)

  EXTERNAL SENDER - Exercise caution with requests, links, and attachments.

Hello, I'm wondering if anyone does the Brown-Hopps Gram stain for gram 
negative bacteria. If so, would you mind to send your protocol please?
Any recommendations for a gram negative (only) stain - specifically E. coli? 
One of our residents is asking.
Thank you!
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[Histonet] (no subject)

[Rhonda McCormick via Histonet]

Hello, I'm wondering if anyone does the Brown-Hopps Gram stain for gram 
negative bacteria. If so, would you mind to send your protocol please?
Any recommendations for a gram negative (only) stain - specifically E. coli? 
One of our residents is asking.
Thank you!
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Re: [Histonet] (no subject)

[Jay Lundgren via Histonet]

IIRC, yes, I seem to remember at AFIP our NCOIC cursing and reprogramming
Roberta every Friday, or for a three day weekend.

Roberta was the VIP's name.  That lovely science-fictiony tan and burnt
orange color scheme.  I wonder what happened to her?  Maybe you bought her?

They never would have let a lowly Airman touch her.

She would call you on the phone though, if her process was interrupted.  No
kidding, look around and you should find a land-line (of course) phone jack
on the back. Pretty cool stuff in 1987.

Sincerely,

Jay A. Lundgren, M.S., HTL (ASCP)

On Thu, Jun 22, 2023 at 8:42 AM Kate Bummer via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Help on how to program an artifact, aka, Miles Tissue Tek VIP 1000 tissue
> processor?
>
> Hello All,
>
> My company purchased the miles tissue tek vip 1000 tissue processor at an
> auction and I have a user manual that is not very useful in figuring out
> how to set up programs.  I was hoping to set up multiple programs but it
> looks like on the automatic it really only takes  1 program and you can set
> up start and end time but cannot set up multiple programs and would have to
> key in a new program every time.  That is ok and not my preferred situation
> as I've really only worked with a Leica ASP300S which of course is much
> newer and can store several programs.
>
> Hoping someone has worked on one of these and may have a user guide that
> is more useful than the user manual I currently have (from 1986 : /  )
>
> Please drop me a line if you have any info it would be much appreciated.
>
>
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[Histonet] (no subject)

[Kate Bummer via Histonet]

Help on how to program an artifact, aka, Miles Tissue Tek VIP 1000 tissue 
processor?

Hello All,

My company purchased the miles tissue tek vip 1000 tissue processor at an 
auction and I have a user manual that is not very useful in figuring out how to 
set up programs.  I was hoping to set up multiple programs but it looks like on 
the automatic it really only takes  1 program and you can set up start and end 
time but cannot set up multiple programs and would have to key in a new program 
every time.  That is ok and not my preferred situation as I've really only 
worked with a Leica ASP300S which of course is much newer and can store several 
programs.

Hoping someone has worked on one of these and may have a user guide that is 
more useful than the user manual I currently have (from 1986 : /  )

Please drop me a line if you have any info it would be much appreciated.


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[Histonet] (no subject)

[Waitts, Celeste via Histonet]

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[Histonet] (no subject)

[Jacobs, Genie via Histonet]

MD Pathology Plano, Texas
2 HT  positions available.  9 pm to 5:30 am Sunday to Friday am and 3:30 am to 
12:00 pm Monday to Friday.
Contact geniejac...@texashealth.org or call 
972-981-3108

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prohibited from copying, distributing, or using the information. Please contact 
the sender immediately by return e-mail and delete the original message from 
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[Histonet] (no subject)

[kendra shultz via Histonet]

   Any suggestions on processing necropsy muscle. Sections are dry and
   pieces are falling off during H We process many other tissue types
   but only have problems with muscle.
   Thanks, Kendra Shultz
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[Histonet] (no subject)

[Michael Bradley via Histonet]

Sent using the mail.com mail app

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[Histonet] (no subject)

[Carnell Burlock via Histonet]

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[Histonet] (no subject)

[Елена Ивлева via Histonet]

I want to unsubscribe 
 
 
С уважением, Ивлева Елена.
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[Histonet] (no subject)

[Maryann Deathridge via Histonet]

Hello All-
We have a DRS 2000 slide stainer (new to us) in our laboratory.  We are 
staining only routine H slides.  I am not sure we have optimal programmed 
established. It seems to stain slower (even though) its double racks.
Does anyone have a routine H protocol (we use clarifier and bluing) 
established that we could use?  I'm thinking maybe we don't have the correct 
icon (exact, infinity) by the correct component.
Any thoughts, ideas appreciated.
If anyone has any experience with this unit please contact me or send me an 
email (madeathri...@pastnashville.com)

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Re: [Histonet] (no subject)

[Dr. Michael Gudo (Morphisto GmbH) via Histonet]

Dear Kate,

very interesting question.

The both substances you mention are a bit difficult in this respect. In a 
general crystal structure phosphortungstic acid has 44 water molecules and 
phosphormolybdic acid 28 molecules of crystal water. This would mean, that for 
a an exact 5% solution you would need around 72,62 g of phosphortungstic acids 
and 72,73 of phosphormolybdic acid on 1.000 ml water. The standard for a 5 % 
solution of a solid substance without crystal water would be 52,63 g per 1.000 
ml.

However, in this case, the substances do not exactly have this number of water 
molecules in the crystal structure because they loose water and they are 
hygroscopic. This means that they take more water molecules from the air and 
that they partly melt in the bottle, if leave it open to long. 
 „• x H20“ - does not mean, that the manufacturers don’t want to give this 
information, its because it cannot be known exactly.

For these substances histological practice is to ignore the crystal water and 
to use the 52,63 g substance for 1.000 ml of water to get a 5% solution (or to 
add 5 g substance to 95 g water).

This does not have an crucial effect, because the mordanting process for which 
these acids are used has to be controlled by microscopy anyway. So if you want 
to make it faster you can add more substance. The important point is only, to 
make it always the same way.

Kind regards
Michael




> Am 24.01.2020 um 16:51 schrieb Kate Davoli via Histonet 
> :
> 
> I'm all set to DYI my own phosphomolybdic/phosphotungstic acid solution for
> running a Masson Trichrome, but I see that the former reagent was
> purchased as "phosphomolybdic acid hydrate".
> 
> All the recipes I have seen call for just "phosphomolybdic acid" but that
> is not a reagent that appears to exist without the water molecules coming
> along for the ride, unless you want to invest in chromatography grade
> stuff, which I think histology folks probably don't routinely do.
> 
> The recipes all call for equal gram amounts of each of these crystals, so
> here's my question:
> 
> Do I calculate how much weight the water is taking up and add more
> phosphomolybdic acid crystals (to account for its tagalong water molecules)
> than called for in the recipe?  Or are these recipes already assuming that
> phosphomolybdic acid HYDRATE is the reagent you have on hand, and I should
> stick with equal amounts?
> 
> This question is somewhat complicated by the fact that the molecular
> formula on the bottle is listed as H3Mo12O40P.XH2O ... which I think means
> the manufacturer won't bet on exactly how many water molecules are involved.
> 
> Any advice appreciated!
> 
> Katherine Davoli, BA, HTL(ASCP)CM
> Supervisor & Lab Manager, Tissue Culture & Histology Core Module
> Ophthalmic and Visual Sciences Research Center
> Department of Ophthalmology, University of Pittsburgh
> Mail Stop Code: EEI010901
> 930 Eye & Ear Institute, 203 Lothrop Street
> Pittsburgh, PA 15213
> (412) 647-8256davol...@upmc.edu and kdav...@gmail.com
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MORPHISTO GmbH
PD Dr. phil. nat. Michael Gudo
Weismüllerstr. 45
60314 Frankfurt am Main
Telefon:069 / 400 3019 - 62
Telefax:069 / 400 3019 - 64

E-Mail: michael.g...@morphisto.de 
Internet: http://www.morphisto.de/ 

Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo

Registergericht: Amtsgericht Frankfurt
Registernummer: HRB 74954
Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199

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[Histonet] (no subject)

[Kate Davoli via Histonet]

I'm all set to DYI my own phosphomolybdic/phosphotungstic acid solution for
running a Masson Trichrome, but I see that the former reagent was
purchased as "phosphomolybdic acid hydrate".

All the recipes I have seen call for just "phosphomolybdic acid" but that
is not a reagent that appears to exist without the water molecules coming
along for the ride, unless you want to invest in chromatography grade
stuff, which I think histology folks probably don't routinely do.

The recipes all call for equal gram amounts of each of these crystals, so
here's my question:

Do I calculate how much weight the water is taking up and add more
phosphomolybdic acid crystals (to account for its tagalong water molecules)
than called for in the recipe?  Or are these recipes already assuming that
phosphomolybdic acid HYDRATE is the reagent you have on hand, and I should
stick with equal amounts?

This question is somewhat complicated by the fact that the molecular
formula on the bottle is listed as H3Mo12O40P.XH2O ... which I think means
the manufacturer won't bet on exactly how many water molecules are involved.

Any advice appreciated!

Katherine Davoli, BA, HTL(ASCP)CM
Supervisor & Lab Manager, Tissue Culture & Histology Core Module
Ophthalmic and Visual Sciences Research Center
Department of Ophthalmology, University of Pittsburgh
Mail Stop Code: EEI010901
930 Eye & Ear Institute, 203 Lothrop Street
Pittsburgh, PA 15213
(412) 647-8256davol...@upmc.edu and kdav...@gmail.com
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[Histonet] (no subject)

[Waitts, Celeste via Histonet]

Question   VIP6SHORT BIOPSY RUNUSING CLEARITE
ANYTHING

Celeste Waitts
Histology Supervisor
Centrastate Medical Center
Freehold, NJ  07728
(732-303-5071)

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Re: [Histonet] (no subject)

[May Wei via Histonet]

   Hi Rhonda,

   To get contact with the local histotech community is a good place to
   start by contacting local community colleges which offer the histotech
   certificate (see the list of the local
   schools [1]https://www.nsh.org/learn/histology-schools). They may even
   want you to teach one of their lab classes given your extensive
   experiences.

   May Wei



    Original Message 
   Subject: [Histonet] (no subject)
   From: Rhonda McCormick via Histonet
   <[2]histonet@lists.utsouthwestern.edu>
   Date: Wed, August 14, 2019 3:10 pm
   To: [3]histonet@lists.utsouthwestern.edu
   Help!
   I became HT certified in 2004 under the HS degree and lab experience
   qualification. I do have a Bachelorâs Degree in Education,, with only
   12 hours of science. I am looking to relocate to Texas and therefore am
   job hunting. The problem Iâm running into is that without an Associates
   Degree or Bachelors in a Science related field, I am not being
   considered for job opportunities even though I have 17 years of
   experience (and am a good histo tech and good employee with great
   references).
   Is anyone else running into this problem? Does the Histonet world
   recommend I go back to school - or just be patient, trusting a job will
   eventually come along?
   Thanks!
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References

   1. https://www.nsh.org/learn/histology-schools
   2. mailto:histonet@lists.utsouthwestern.edu
   3. mailto:histonet@lists.utsouthwestern.edu
   4. mailto:Histonet@lists.utsouthwestern.edu
   5. http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Re: [Histonet] (no subject)

[Morken, Timothy via Histonet]

Rhonda, that is unfortunate. It seems with the shortage of histotechs in most 
places that experience would be valued. Certainly you qualify since you were 
certified under the rules at the time. But institutions can set their own 
requirements as well which may be more strict. I wonder if your applications 
are being kicked out by automated programs that simply reject applications if 
they don't find certain terms.  Maybe the managers in the department never even 
see your application. I have had applicants in this situation and work with my 
HR department to have them clear such applicants so I can at least get them in 
the system for consideration even if they don't meet all requirements.


Maybe try to find out real people to contact at the institutions pathology 
department and deliver resumes directly to them rather than thru the online 
application system they may have. That way they can have their HR send your 
application through.


Tim Morken


From: Rhonda McCormick via Histonet 
Sent: Wednesday, August 14, 2019 3:10:02 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [Histonet] (no subject)

Help!

I became HT certified in 2004 under the HS degree and lab experience 
qualification.  I do have a Bachelor’s Degree in Education,, with only 12 hours 
of science.  I am looking to relocate to Texas and therefore am job hunting.  
The problem I’m running into is that without an Associates Degree or Bachelors 
in a Science related field, I am not being considered for job opportunities 
even though I have 17 years of experience (and am a good histo tech and good 
employee with great references).
Is anyone else running into this problem? Does the Histonet world recommend I 
go back to school - or just be patient, trusting a job will eventually come 
along?
Thanks!
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[Histonet] (no subject)

[Rhonda McCormick via Histonet]

Help!

I became HT certified in 2004 under the HS degree and lab experience 
qualification.  I do have a Bachelor’s Degree in Education,, with only 12 hours 
of science.  I am looking to relocate to Texas and therefore am job hunting.  
The problem I’m running into is that without an Associates Degree or Bachelors 
in a Science related field, I am not being considered for job opportunities 
even though I have 17 years of experience (and am a good histo tech and good 
employee with great references). 
Is anyone else running into this problem? Does the Histonet world recommend I 
go back to school - or just be patient, trusting a job will eventually come 
along?
Thanks!
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[Histonet] (no subject)

[Cindy Bird via Histonet]

Can anyone in the Dallas area tell me how they dispose of glass slides and what 
the regulations are?
Cindy Bird
Anatomical Medical Laboratories, Inc.
1600 Scripture Street
Denton, TX  76201
940-384-6210
940-384-6000
Fax 940-565-9588

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Re: [Histonet] (no subject)

[MARY ANN via Histonet]

Cindy I can message you about it


Sent from Xfinity Connect Application

-Original Message-

From: histonet@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Sent: 2018-10-02 8:31:45 AM 
Subject: [Histonet] (no subject)


Can anyone out in histo land give me feedback on the IHC instrument by StatLab 
Quantum HDX and how it compares to other automated platforms.  Thanks in 
advance!


Cindy Bird
Anatomical Medical Laboratories, Inc.
1600 Scripture Street
Denton, TX  76201
940-384-6210
940-384-6000
Fax 940-565-9588

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Re: [Histonet] (no subject)

[Bryan Llewellyn via Histonet]

All of the Picro-Mallory variants are trichrome stains. An explanation 
of how they work is here:


http://stainsfile.info/StainsFile/theory/tri_gen.htm
http://stainsfile.info/StainsFile/stain/fibrin/fibrin.htm

Follow the links as well for added information.

Bryan Llewellyn



Пешков Максим via Histonet wrote:


Dear Histonetters!
I need in your proffessional help.
Can you explain for me a the chemical mechanism of Picro-Mallory V stain by 
chemcial language?
I will appreciate references about this issue except original article of 
Lendrum A.C. et al (1962)  
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC480427/pdf/jclinpath00070-0009.pdf
Before asking I was read some histotechnological books, but can not find it. They 
are: Bancroft (from 5 to 8th ed), F. Carson A Self-instructional text 3 
ed, C.F.A. Culling (3rd ed), AFIP manuals (3-4 ed), Woods and Ellis (Histology lab: 
A complete reference), Lilli RD, 1962, Romeis 2014 (18 auflage) and some others 
book.
-- Russia,
Taganrog,
Maxim Peshkov.
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[Histonet] (no subject)

[Пешков Максим via Histonet]

Dear Histonetters!
I need in your proffessional help.
Can you explain for me a the chemical mechanism of Picro-Mallory V stain by 
chemcial language?
I will appreciate references about this issue except original article of 
Lendrum A.C. et al (1962)  
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC480427/pdf/jclinpath00070-0009.pdf 
 
Before asking I was read some histotechnological books, but can not find it. 
They are: Bancroft (from 5 to 8th ed), F. Carson A Self-instructional 
text 3 ed, C.F.A. Culling (3rd ed), AFIP manuals (3-4 ed), Woods and Ellis 
(Histology lab: A complete reference), Lilli RD, 1962, Romeis 2014 (18 auflage) 
and some others book.
-- Russia,
Taganrog,
Maxim Peshkov.
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[Histonet] (no subject)

[Cindy Bird via Histonet]

Can anyone out in histo land give me feedback on the IHC instrument by StatLab 
Quantum HDX and how it compares to other automated platforms.  Thanks in 
advance!


Cindy Bird
Anatomical Medical Laboratories, Inc.
1600 Scripture Street
Denton, TX  76201
940-384-6210
940-384-6000
Fax 940-565-9588

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[Histonet] (no subject)

[Cindy Bird via Histonet]

Hello All,

I am curious to see what chemical everyone uses to decal bone marrow biopsies?

Cindy Bird
Anatomical Medical Laboratories, Inc.
1600 Scripture Street
Denton, TX  76201
940-384-6210
940-384-6000
Fax 940-565-9588

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[Histonet] (no subject)

[Иванов Иван via Histonet]

   --
   ÐÑпÑавлено из мобилÑного пÑиложениÑ
   ЯндекÑ.ÐоÑÑÑ
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[Histonet] (no subject)

[seaduster--- via Histonet]

Hey Histonet https://goo.gl/pmPPmg   
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[Histonet] (no subject)

[Stella Mireles via Histonet]

Please unsubscribe me. I tried this on your site, but still receiving
messages.
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[Histonet] (no subject)

[Steve McClain via Histonet]

If it is any help, A dollar bill weighs 1 gram and a quarter weighs 5.67 grams. 
It is simpler to write up some calibration procedure based on knowns. 

Steve A. McClain, MD

> On Jun 8, 2017, at 13:20, "histonet-requ...@lists.utsouthwestern.edu" 
>  wrote:
> 
> Balance calibration
> Message-ID:

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[Histonet] (no subject)

[Jamal Rowaihi via Histonet]

Hi colleagues We are going for Digital Pathology What is your recommendation 
What's the best device and company (Aperio, Philips, 3DHISTECH)

Regards
Jamal RowaihiAnatomic Pathology SupervisorAl Borg Medical Laboratories Sent 
from my cell phone
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[Histonet] (no subject)

[Sam Kiousis via Histonet]

Does anyone know the histo standard antibodies for differentiating sarcoma from 
glial components in a gliasarcoma cell line Ive been growing?  Any help would 
be appreciated


Thanks


Sam Kiousis
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[Histonet] (no subject)

[Salomao Segal via Histonet]

I would appreciate if anyone could direct me to protocols for processing
large slabs of tissue such as whole human brain slabs.  I have acquired a
very large microtome and knife but no previous experience with embedding,
cutting, mounting and cover slipping such large sections.  Any
information/protocols/resources would be greatly appreciated.



*Solomon Segal, M.D.*



*Associate Professor of Anatomy Department of AnatomyKirksville College of
Osteopathic MedicineA.T. Still University*

*800 W. Jefferson St.*
*Kirksville, MO  63501-1497*
*e-mail*: s olomonse...@atsu.edu

www.atsu.edu
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Re: [Histonet] (no subject)

[Blazek, Linda via Histonet]

Try the BioCare antibody.  It's excellent.

-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, December 01, 2016 1:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Since, Cell Marque H. Pylori is not available,  can some one give a comparable 
antibody to order?  Thanks in advance


Nirmala Srishan








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[Histonet] (no subject)

[Nirmala Srishan via Histonet]

Since, Cell Marque H. Pylori is not available,  can some one give a 
comparable antibody to order?  Thanks in advance


Nirmala Srishan








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[Histonet] (no subject)

[Kuhnla, Melissa via Histonet]

Hello,
We looking to bring Kappa and Lambda by CISH on board here in my laboratory.  
We have Ventana Benchmark Ultra instruments.  Can anyone running these tests 
let me know how long your staining protocol is?  I am putting together a report 
on how this change will impact workflow and turn around times.  Thank you all.

Melissa Kuhnla
Lead Medical Technologist
for IHC and FISH testing
Regional Laboratory Services
Good Samaritan Hospital
631-609-2551

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[Histonet] (no subject)

[Cindy Bird via Histonet]

Anyone know where I can find the "old" special stain description sheets?  The 
ones we used back in the day when studying to take exam.

Cindy Bird
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Re: [Histonet] (no subject)

[Duddey, Aimee via Histonet]

We ran into this situation with TJC.  We had a very limited check off sheet.  
In effort to standardize processes across the laboratory we adopted a program 
very similar to the NY facility whose program is referenced in the TJC leading 
practice library.  It is very rigorous and time consuming but serves its 
purpose well.  Also, CAP has a competency assessment program specifically for 
histology that is a subscription based purchase regardless if you are 
accredited by them.

Aimee

-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 01, 2016 1:49 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi,

What is everyone doing about the Histology Staff competencies.  I know CLIA has 
several criteria for the med-tech competencies.  Since the Histotechs do not 
report out results, how is everyone implementing this process.  Any information 
is greatly appreciated.


Nirmala 







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Re: [Histonet] (no subject)

[Morken, Timothy via Histonet]

Nirmala, Histotechs are not required to have competency assessment under CLIA 
regulations because, as you note, they do not interpret any tests or report any 
results. However, if they do grossing then they do require high complexity 
competency assessment.

Having said that, if there is some concern in an inspection, any deemed 
accreditation agency (CLIA, CAP or JC) inspector, or state inspector,  can ask 
how you determine competency of any lab staff member to do any task. It is up 
to the Laboratory Director to determine how that is done, and how often. An 
institution may do it differently than the standard CLIA regulations outline, 
less often and less rigorous, but will need to convince an inspector that it is 
acceptable. The easiest way is to be sure there will not be any problems is to 
follow the competency assessment criteria for medium and high complexity - that 
is accepted and familiar to the inspectors so they should have no problem 
accepting it for other staff. 

Tim Morken
Pathology Site Manager, Parnassus 
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center




-Original Message-
From: Nirmala Srishan via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, September 01, 2016 10:49 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi,

What is everyone doing about the Histology Staff competencies.  I know CLIA has 
several criteria for the med-tech competencies.  Since the Histotechs do not 
report out results, how is everyone implementing this process.  Any information 
is greatly appreciated.


Nirmala 







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[Histonet] (no subject)

[Nirmala Srishan via Histonet]

Hi,

What is everyone doing about the Histology Staff competencies.  I know 
CLIA has several criteria for the med-tech competencies.  Since the 
Histotechs do not report out results, how is everyone implementing this 
process.  Any information is greatly appreciated.


Nirmala 







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[Histonet] (no subject)

[Scott, Catherine L via Histonet]

UT Path located in the medical center (Houston) is currently looking for a 
Medical Technologist that is able to work on an as needed basis, hours are 
negotiable. Duties include, but not limited to performing molecular testing 
using the Hologic Panther system, this could lead to a full time position for 
the right candidate.

In addition, to the MT position, we also have available a full time HTL 
position performing immunohistochemical staining utilizing the Dako system.

We offer completive salaries and great benefits.  Please contact me at 
713-500-5401 or Catherine.L.Scott @uth.tmc.edu.


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[Estela Martinez via Histonet]

Hello My Histo Friends,

Does anybody know if OSHA has separate giude lines for Histology?  We are 
having to wear gloves and face shields when cutting and also special shoes in 
the gross area because of knifes and blades.   The main lab has to do all this 
per OSHA and was wondering if there are separate guide lines for Histology 
Department.  Can somebody please help me!  Thanks in advance!!


Estela Martinez
Histology Supervisor
Medical Center Hospital
Odessa, TX 79761
432-640-2348
emartin...@echd.org
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[Histonet] (no subject)

[Nirmala Srishan via Histonet]

Hi All. 

If anyone is doing the ER/PGR Immnunohistochemistry TMA with CAP, what 
have you been doing when when they grade some cores as code 27 ?  If any 
one has any input regarding this, I would greatly appreciate it. 
CAP provide the slides, but one of our slides were missing some cores.

Thanks

Nirmala










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[Histonet] (no subject)

[Cindy Bird via Histonet]

Hello All,

Is there anyone in Histoland that still uses an anti-roll plate for their 
cryostats. We are issuing "hanging up "

Cindy
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[Histonet] (no subject)

[Estela Martinez via Histonet]

Hey Leslie,

This is the link I was talking about.  histonet@lists.utsouthwestern.edu

I have to warn you, you will get lots of emails from people asking questions 
and others answering but I really like seeing all the different comments people 
put.


Estela Martinez
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[Histonet] (no subject)

[Estela Martinez via Histonet]

Good Morning All,

Does anybody have a sample of CAP Quality Indicator Monitring Guidance that 
they can share an example with me.  The new form is a little more complex and I 
would like to see samples.  Thanks in advance!!


Estela Martinez
Histology Supervisor
emartin...@echd.org
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[Histonet] (no subject)

[Eck, Allison via Histonet]

How many of you have a cytology dept where your cyto techs do all their own 
prep and reports?  If you don't, do you have a cyto prep aide or does a histo 
tech help?

Also, how many of you have histo and cyto as the same department with an AP 
manager?  Does the AP manager oversee transcription as well

Thank you in advance



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[Histonet] (no subject)

[Amos, Lori (VDH) via Histonet]

I have a beyond repair tissue processor that needs to be dispose of.  It has 
not been in use for a year. All the solutions have been emptied but the 
paraffin has solidified in the chambers. We found a recycling company that is 
willing to remove it for us but they are requiring us to first prove that it is 
not  biohazardous. How does one dispose of their tissue processor?

Lori


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[Histonet] (no subject)

[ODea, Elise via Histonet]

Thanks for your input. I am thankful we do not have as many disruptions as your 
lab!   Some sort of guidelines are  necessary to evaluate in some labs for new 
histotechs to aspire to.

Elise
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[Histonet] (no subject)

[Merrill, Dawn S via Histonet]

Can you please unsubscribe me to this mailing list.
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[Angela Davis via Histonet]

Pls unsubscribe me
Thank you!
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[Histonet] (no subject)

[Teresa Harris via Histonet]

Yes
Sent from my iPhone

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[Histonet] (no subject)

[dianna uhrich via Histonet]

Hi 

 

Need some advise on tissue cassette printers.

 

What is everyone using, and what problems does everyone have
  
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[Histonet] (no subject)

[Karen Stephanson]

Our Histology laboratory currently uses Mercurochrome to mark specimens, but we 
need to discontinue using it.  Can anybody tell me of a good substitute. 
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[Histonet] (no subject)

[srishan]

Hi All,

Does anyone know where we could get the original grey antibody storage 
racks used to store antibodies in the refrigerator?  We are using ventana 
antibodies. 
Thanks in advance.

Mala









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[Histonet] (no subject)

[Bruijntjes, J.P. (Joost)]

Thanks to all who responded on my request for CD-4; CD-8a and CD68 staining on 
mouse formalin fixed, paraffin embedded tissues

Joost Bruijntjes
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[Histonet] (no subject)

[Carolyn Nelson]

Hi, I was hoping someone can help me with tissue falling off the slides. I have 
tried regular slides with and without adhesive in the water bath. Charged 
slides with and without adhesive in the water bath. I have not changed the type 
of slides I’m using. All the chemicals are fresh in the processor and the stain 
line, as well as the paraffin in the processor. It is the worst on needle bx ( 
prostate and breast ). I am SO frustrated, any help would be greatly 
appreciated! 


Carolyn






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RE: [Histonet] (no subject)

[Roy, Lisa]

How long do you bake slides for before staining, at what temperature?  Does 
your stainer use agitation?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carolyn Nelson
Sent: Thursday, April 30, 2015 11:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hi, I was hoping someone can help me with tissue falling off the slides. I have 
tried regular slides with and without adhesive in the water bath. Charged 
slides with and without adhesive in the water bath. I have not changed the type 
of slides I’m using. All the chemicals are fresh in the processor and the stain 
line, as well as the paraffin in the processor. It is the worst on needle bx ( 
prostate and breast ). I am SO frustrated, any help would be greatly 
appreciated! 


Carolyn






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Re: [Histonet] (no subject)

[Shruti Shah]

Hi does any one doing mice tibia histology, we use to fix in formalin for 24 
hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 
24 hours long protocol in automatic processor.
But I am facing problem with bone marrow shrinkage. If any one have idea for 
decalcification timing and solution can resolved this problem and keep bone 
marrow intact with bone.
Thank you in advance.

Regards,
Shruti

Sent from my iPhone

 On 21 Apr 2015, at 8:51 am, Garrey Faller garr...@gmail.com wrote:

 Here is the CAP checklist requirement:
 ANP.21450
 All  histochemical stains are of adequate quality, and daily controls are
 demonstrated on each day of use for the tissue components or organism for
 which they were designed.

 Ray...you should call the CAP and ask for guidance on this.
 My interpretation of this requirement is that it should be OK to use a
 fungus from an orange peel. An orange peel fungus should have the same
 staining characteristics as a candida or aspergillus etc.  Similarly a
 bacteria is a bacteria. If you can produce a control that has both gram
 positives and negatives, it should be OK. But, don't quote me on this.

 Call the CAP for a definitive answer. I am interested in their response.
 Garrey

 On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote:

 I asked about this in a different vein months ago.  Has anyone shown a
 strawberry or ground meat or slim jim or orange peel as a bacteria/fungus
 control used for diagnostics to an inspector inspecting the lab and was
 there any comment from the inspector either positive or negative. Never
 heard back anything.
 Ray, Lake Forest Park, WA

 - Original Message -

 From: tjfinney2...@gmail.com
 To: histonet@lists.utsouthwestern.edu
 Sent: Sunday, April 19, 2015 5:24:53 PM
 Subject: [Histonet] (no subject)

 GMS controls
 From my understanding we can't use non human controls on patients. I
 could be wrong, but you may want to look into it.

 Happy Connecting.  Sent from my Sprint Phone.

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[Histonet] (no subject)

[tjfinney2010]

GMS controls
From my understanding we can't use non human controls on patients. I could be 
wrong, but you may want to look into it. 

Happy Connecting.  Sent from my Sprint Phone.
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Re: [Histonet] (no subject)

[koellingr]

I asked about this in a different vein months ago.  Has anyone shown a 
strawberry or ground meat or slim jim or orange peel as a bacteria/fungus 
control used for diagnostics to an inspector inspecting the lab and was there 
any comment from the inspector either positive or negative. Never heard back 
anything. 
Ray, Lake Forest Park, WA 

- Original Message -

From: tjfinney2...@gmail.com 
To: histonet@lists.utsouthwestern.edu 
Sent: Sunday, April 19, 2015 5:24:53 PM 
Subject: [Histonet] (no subject) 

GMS controls 
From my understanding we can't use non human controls on patients. I could be 
wrong, but you may want to look into it. 

Happy Connecting.  Sent from my Sprint Phone. 

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[Histonet] (no subject)

[Nan Gray]

please unsubscribe Live with Love, Joy and Vibrant Health    
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[Histonet] (no subject)

[Chapman, Cherie J.]

Does anyone know for CAP accreditation if you can still use a regular microwave 
in a lab?

Thanks,

Cherie

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RE: [Histonet] (no subject)

[Kelley, Amanda]

Hello my friend, I hope you are well.  Sorry Cherie, but the CAP checklist 
requires that you use a Lab grade microwave.   I would recommend that you order 
the BP-110 laboratory grade microwave,  it must be vented.  Since you're in 
Missouri, I would recommend contacting Lab Storage systems in St. Peter's Mo. 
1-800-345-4167

Amanda Kelley HTL
Dermatopathology Center at Washington University
Cortex One Suite 212
Phone: 314-362-5759
Fax: 314-362-5701
akel...@path.wustl.edu




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie 
J.
Sent: Thursday, February 19, 2015 2:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Does anyone know for CAP accreditation if you can still use a regular microwave 
in a lab?

Thanks,

Cherie

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[Histonet] (no subject)

[dianna uhrich]

I work in a small hospital and we our having a hard time justifing the amount 
of people

that work in our area.  How do people figure this out, what do people do

for FTE's 

 

Thanks for your help

Dianna
  
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[Histonet] Sticky subject responses...Thank you

[Davis, Cassie]

To all you wonderful folks who responded, a big thanks! I've been doing histo 
since 1990 and NEVER had static like today, it's not usually that big of a 
problem in this mid-Atlantic state. When it does occur the tricks I named 
earlier usually help but I am always up for learning something new. I will try 
the low tech option of moist something in my clean-up tray tomorrow, first. I 
usually can't stand anything in my tray after facing but if given a choice I'll 
suck it up. I will keep you posted. Thanks again!

Cassandra Davis
cda...@che-east.org
302-575-8095



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Re: [Histonet] (no subject)

[Rene J Buesa]

Yes, you have to. The origin of the section (MOHS or other) is not the criteria 
to use controls, but the process is.René J.  

 On Tuesday, December 2, 2014 4:23 PM, Chapman, Cherie J. 
chapm...@health.missouri.edu wrote:
   

 Does anyone know if you have to use controls for IHC on MOHS frozen section 
procedures to satisfy CAP requirements?

Thank you,

Cherie Chapman, BS, HT, HTL (ASCP)
Associate Director of Dermatopathology Laboratory
University of Missouri Department of Dermatology
University Physicians Medical Building
Phone: (573) 884-0123
Fax: (573) 884-0834

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[Histonet] (no subject)

[Chapman, Cherie J.]

Does anyone know if you have to use controls for IHC on MOHS frozen section 
procedures to satisfy CAP requirements?

Thank you,

Cherie Chapman, BS, HT, HTL (ASCP)
Associate Director of Dermatopathology Laboratory
University of Missouri Department of Dermatology
University Physicians Medical Building
Phone: (573) 884-0123
Fax: (573) 884-0834

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RE: [Histonet] (no subject)

[Patsy Ruegg]

I would think you would have to validate IHC for MOHS the same as FFPE, once 
the validation process is done maybe controls would not be necessary.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
prueg...@hotmail.com
pru...@ihctech.net


 From: chapm...@health.missouri.edu
 To: histonet@lists.utsouthwestern.edu
 Date: Tue, 2 Dec 2014 21:22:38 +
 Subject: [Histonet] (no subject)
 
 Does anyone know if you have to use controls for IHC on MOHS frozen section 
 procedures to satisfy CAP requirements?
 
 Thank you,
 
 Cherie Chapman, BS, HT, HTL (ASCP)
 Associate Director of Dermatopathology Laboratory
 University of Missouri Department of Dermatology
 University Physicians Medical Building
 Phone: (573) 884-0123
 Fax: (573) 884-0834
 
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[Histonet] (no subject)

[Bruijntjes, J.P. (Joost)]

Hi all

Is anyone of you using a an alternative for the DAKO-ARK kit. In my opinion it 
is good, but rather expensive.

Best regards,
Joost Bruijntjes

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[Histonet] (no subject)

[Sophia Lin]

We are a small GI laboratory in Murrieta, CA looking for a part time
histotechnician. Certification suggested, but not required. Looking for
dedicated and committed person who is a team player and can work well with
others.

Please submit applications to lins0...@gmail.com

Thanks!
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[Histonet] (no subject)

[Travis Troyer]

I was wondering what facility labs are sending nerve and muscle biopsies to.


 

Thanks,

Travis Troyer

Peterson Laboratory Services

Manhattan, KS

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RE: [Histonet] (no subject)

[WILLIAM DESALVO]

University of Michigan MLabs

William DeSalvo, BS HTL(ASCP)
 
 From: ttro...@petersonlab.com
 To: histonet@lists.utsouthwestern.edu
 Date: Wed, 10 Sep 2014 12:22:55 -0500
 Subject: [Histonet] (no subject)
 
 I was wondering what facility labs are sending nerve and muscle biopsies to.
 
 
  
 
 Thanks,
 
 Travis Troyer
 
 Peterson Laboratory Services
 
 Manhattan, KS
 
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Re: [Histonet] (no subject)

[Karen]

You could send them to Beaumont Health System. 

Sent from my iPhone

 On Sep 10, 2014, at 1:31 PM, WILLIAM DESALVO wdesalvo@outlook.com wrote:
 
 University of Michigan MLabs
 
 William DeSalvo, BS HTL(ASCP)
 
 From: ttro...@petersonlab.com
 To: histonet@lists.utsouthwestern.edu
 Date: Wed, 10 Sep 2014 12:22:55 -0500
 Subject: [Histonet] (no subject)
 
 I was wondering what facility labs are sending nerve and muscle biopsies to.
 
 
 
 
 Thanks,
 
 Travis Troyer
 
 Peterson Laboratory Services
 
 Manhattan, KS
 
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[Histonet] (no subject)

[Ronda Mire]

Hello Netters,

 

Anyone happen to know what the recommended humidity level range is for
Methylmethacrylate infiltration and embedding is?  How about any
publications that covers this topic?  Thanks

 

 

Ronda Mire

Laboratory Manager/Chief Technologist

CVPath Institute

19 Firstfield Rd

Gaithersburg, MD 20878

301-208-3570 x112

rm...@cvpath.org

 

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RE: [Histonet] (no subject)

[Michael LaFriniere]

My favorite coverslipper at this time  is Leica due to needing glass cover 
slipping for imaging.

Michael
Michael R. LaFriniere, HT (ASCP) 
Executive Director
 

Capital Choice Pathology Laboratory
12041 Bournefield Way, Suite A . Silver Spring, MD 20904  
P: 240.471.3427 . F: 240.471.3401 . Cell 410-940-8844
michael.lafrini...@ccplab.com
 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Saturday, July 26, 2014 11:32 AM
To: Aneesh Dhiman; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

Your request is quite odd. Are you looking for 5 guys to recommend or are you 
looking for a good coverslipper?
This is not an issue of obtaining 5 guys with opinions; what if the 5 guys 
advise you to buy the worst coverslipper?
I think you need to know the quality of the instrument first.
You should contact manufacturers and read specifications. Later you may select 
2 and submit those 2 to find out which of the two, according with the histonet 
guys is preferable.
I prefer Sakura.
There you have it: the opinion of one guy
René J. 


On Friday, July 25, 2014 4:21 PM, Aneesh Dhiman aneeshdhi...@gmail.com wrote:
  


Hi guys,  I need to settle on a coverslip machine, I wonder which is most
reliable need 5 peoples at minimum or 10 opinions for the recommendations
please
Aneesh Dhiman
University of Alberta hospital
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RE: [Histonet] (no subject)

[Sanders, Jeanine (CDC/OID/NCEZID)]

not odd at all.does it matter if it is a glass coverslipper or film? We 
have a Sakura glass coverslipper and a Leica glass coverslipper. Both have been 
great. 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Saturday, July 26, 2014 11:32 AM
To: Aneesh Dhiman; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] (no subject)

Your request is quite odd. Are you looking for 5 guys to recommend or are you 
looking for a good coverslipper?
This is not an issue of obtaining 5 guys with opinions; what if the 5 guys 
advise you to buy the worst coverslipper?
I think you need to know the quality of the instrument first.
You should contact manufacturers and read specifications. Later you may select 
2 and submit those 2 to find out which of the two, according with the histonet 
guys is preferable.
I prefer Sakura.
There you have it: the opinion of one guy
René J. 


On Friday, July 25, 2014 4:21 PM, Aneesh Dhiman aneeshdhi...@gmail.com wrote:
  


Hi guys,  I need to settle on a coverslip machine, I wonder which is most 
reliable need 5 peoples at minimum or 10 opinions for the recommendations 
please Aneesh Dhiman University of Alberta hospital 
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RE: [Histonet] (no subject)

[Cooper, Brian]

I agree. The Sakura tape coverslippers are work horses and will not disappoint. 
Stick with Sakura's brand of tape as well. There are other brands of tape 
available, but we found them to be of terrible quality.

Brian Cooper
CHLA Histology Supervisor

-Original Message-
From: Rene J Buesa [rjbu...@yahoo.com]
Received: Saturday, 26 Jul 2014, 8:38AM
To: Aneesh Dhiman [aneeshdhi...@gmail.com]; histonet@lists.utsouthwestern.edu 
[histonet@lists.utsouthwestern.edu]
Subject: Re: [Histonet] (no subject)

Your request is quite odd. Are you looking for 5 guys to recommend or are you 
looking for a good coverslipper?
This is not an issue of obtaining 5 guys with opinions; what if the 5 guys 
advise you to buy the worst coverslipper?
I think you need to know the quality of the instrument first.
You should contact manufacturers and read specifications. Later you may select 
2 and submit those 2 to find out which of the two, according with the histonet 
guys is preferable.
I prefer Sakura.
There you have it: the opinion of one guy
René J.


On Friday, July 25, 2014 4:21 PM, Aneesh Dhiman aneeshdhi...@gmail.com wrote:



Hi guys,  I need to settle on a coverslip machine, I wonder which is most
reliable need 5 peoples at minimum or 10 opinions for the recommendations
please
Aneesh Dhiman
University of Alberta hospital
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[Histonet] (no subject)

[Aneesh Dhiman]

Hi guys,  I need to settle on a coverslip machine, I wonder which is most
reliable need 5 peoples at minimum or 10 opinions for the recommendations
please
Aneesh Dhiman
University of Alberta hospital
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[Histonet] (no subject)

[awill.imdpath...@yahoo.com]

Please remove me 

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RE: [Histonet] (no subject)

[Weems, Joyce K.]

Hello,

You will need to do that yourself at 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Best wishes!

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
awill.imdpath...@yahoo.com
Sent: Thursday, June 12, 2014 1:55 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Please remove me

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RE: [Histonet] (no subject)

[Anne Murvosh]

I just got back from the Mohs conference.  The Biocare people have the stain 
and told me they would come out to help work it up. Anne

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bethany
Sent: Thursday, May 29, 2014 1:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

The doctor I work for wants to use Mart 1 staining for Mohs on melanoma cases.
Any information on where to start? What equipment do we need? Where do we 
acquire the stains/antibodies, etc?
thx
Bethany
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[Histonet] (no subject)

[Bethany]

The doctor I work for wants to use Mart 1 staining for Mohs on melanoma cases.
Any information on where to start? What equipment do we need? Where do we
acquire the stains/antibodies, etc?
thx
Bethany
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[Histonet] (no subject)

[cynthia haynes]

My name is Cynthia James; I work in a histopathology lab in Melrose Park 
Illinois. We are told to change the solution(2-Methyl Butane) which we use in 
the Histobath to freeze tissues for frozen sections to something less 
flammable. I remember a solution was discussed that can be used instead of the 
which is less flammable than the  IsoPentane that we are currently using. 
Would someone contact at 708-681-3200 ext:2012 or 2036 and tell me more about 
this solution.I am available from 5:00 am to 1:30 pm monday-friday central 
time, if you know the company that is selling this solution please let me know.
 
Thank you in advance
Cynthia James
 
P.S I can also be reached at this email address naje1...@yahoo.com or 
cynthia_haynes-ja...@luhs.org
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[Histonet] (no subject)

[histogirl4]

Job Opportunity: Pathology Associates Of Greenville Texas is seeking a 
full-time Histotehnologist/Histotechnichian, to join out practice.

We are a free standing anatomic pathology laboratory with hospital 
affiliations, staffed by five pathologists and two full-time 
Histotechnologists/Histotechnicians. Our laboratory is fully automated. We 
preform routine surgical pathology, few non-gyn cytology, special stains 
(Histochemistry) and Immunohistchemistry (IHC).

Requirements for the successful applicant: Strong work ethics- Excellent 
Interpersonal Skills and willing to assist in Frozen sections and Maintain 
Policies and Procedures of The Histology Laboratory. Candidates are preferably 
ASCP Certified/Eligible.

Interested applicants may fax their resume at 903-454-1716

E-mail address: eb...@yahoo.com






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[Histonet] (no subject)

[histogirl4]

Job Opportunity: Pathology Associates of Greenville is seeking a full-time 
Histotechnologist/Histotechnician, to join practice.

We are a free-standing anatomic pathology laboratory with hospital 
affiliations, staffed by five pathologists and two full-time 
Histotechnologists/Histotechnicians. Our laboratory is fully automated, We 
perform routine surgical pathology, few non-gyn cytology, special stains 
(Histochemisty) and Immunhistochemistry (IHC). Requirements for the successful 
applicant-- Strong Work Ethics_ Excellent Interpersonal Skills and willinf to 
assit in Frzen Sections and maintaining policies and procedures of The 
Histology Laboratory, Canidates are preferably ASCP Certified/Eligible, 
Intereseted applicants may fax their resume to 903-454-1716.

E-mail addreass: eb...@yahoo.com






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[Histonet] (no subject)

[Leann M. Murphy]

How is everyone validating the tissue processor for new CAP ANP.23045 question 
on function and verification of equipment?



LeAnn Murphy

Aultman Hospital

Canton, Ohio






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RE: [Histonet] (no subject)

[joelle weaver]

What I did recently for two new processors, conventional type-
I  did parallel trial slides of multiple tissue types ( same types as patients) 
 for fixation, morphology , processing artifacts for 9 programs.
I grossed them in and recorded fixation times, type, thickness,  overall 
dimensions. I ran on the test programs. Then I embedded and sectioned and 
evaluated the  results by microscopic review by techs  then the medical 
director of  the H  E stained sections for each program and tissue type. 
Looking at any autolysis, nuclear detail, poor dehydration, other processing 
problems in each set. 
 
Then I just made a simple evaluation sheet for any tissue processing related 
issues, with a number rating/scale for the results. Retained records of the 
validation runs and the stained sections used for validation. Defined 
acceptable tissue types  and dimensions for the processing programs in the SOP, 
 and then I just created back up/recovery procedure and reprocessing procedure 
and ran through those for comparison. When completed, I just compiled into 
validation summary report. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: lmurp...@aultman.com
 To: Histonet@lists.utsouthwestern.edu
 Date: Thu, 3 Apr 2014 15:26:17 +
 CC: 
 Subject: [Histonet] (no subject)
 
 How is everyone validating the tissue processor for new CAP ANP.23045 
 question on function and verification of equipment?
 
 
 
 LeAnn Murphy
 
 Aultman Hospital
 
 Canton, Ohio
 
 
 
 
 
 
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[Histonet] (no subject)

[Victor Gravley]

Please remove us from the mailing list

Sent from my iPhone

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[Histonet] (no subject)

[Amos Brooks]

Hi Tresa,
 I haven't seen any responses to this question, so I figure I should take a 
crack at it.
 You are right that using a polymer detection system is so clean that you 
hardly have much to worry about background/non-specific staining when not using 
a buffer for wash steps. There is however a good reason to use them anyway.
 To explain this it might help to think of two slinkey springs laying 
parallel to each other. You need to get them to slide together as perfectly as 
possible. If they are moving around like slinkeys always do, this will be a 
difficult thing to accomplish. You will need to get them to sit still so that 
when you push them together they will just slide nicely into place.
 Taking this analogy to the realm of IHC that we are discussing, the two 
slinkeys are obviously the antibody and antigen. The wiggling and jiggling that 
they are doing is due to Van der Walls forces (I probably misspelled that). Now 
we usually have a few strategies to make these jiggley antibodies behave. 
Having a good antibody diluent helps a lot, as does having an antibody that has 
a high affinity and avidity to the antigen. It also helps to have the keep the 
reaction at a constant pH and tonicity. That is where the wash buffer comes in. 
It can keep the non-specific staining out when using a avidin-biotin reaction, 
but it also makes the antigen-antibody reaction more comfortable.
 So, yes you will surely see some decent results with distilled water 
washes especially with antibodies that are really attracted to each other, but 
for the ones that need some more coaxing or the ones that you are *really* 
depending on specificity for quantitation (her2, ER, PR etc), you will really 
want to make sure you have given these two love bird slinkeys as much chance to 
hook up a  possible.
 I hate trying to explain something like this without citing a good source 
for where I got this from. In looking around there isn't much out there 
explaining the Why of many IHC procedures. So much of modern medicine is 
voodoo just follow the procedure, dont ask questions! This is why I always 
recommend the Dako IHC manuals. They give such explanations without being so 
verbose you want to put your eyes out. So for more discussion on this subject 
check out  the Dako IHC manual 5th edition pg 122 or thereabouts.
 I hope this helps explain why I feel the buffer steps are essential and 
really shouldn't be replaced by water rinses. It is a really good question 
though since we are all trying to save a little money and with how much 
companies are charging for these buffers, it's a good place to look. IHCworld 
has some great recipes for making them up yourself and they will save you a 
fortune.

Have a great weekend,
Amos


Message: 1 Date: Thu, 13 Mar 2014 17:16:52 + From: Goins, Tresa 
tgo...@mt.gov Subject: [Histonet] IHC without wash buffer To: 
histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Message-ID: fa81e534f40def44a70f0fbbcd49d2e425d47...@doaisd5235.state.mt.ads 
Content-Type: text/plain; charset=us-ascii

In a continuing effort to limit the volume of reagent used in each step of a 
manual IHC, I have tried TBS and TBST on slides with barriers (expensive) and 
without barriers. The results were not stellar with the barrier slides - the 
reagent still escapes. We dewax with hot detergent (may be a contributing 
factor) and the Tween 20 in the TBST definitely alters the hydrophobicity of 
the barrier, an effect that is not reversed with a water wash.

Consequently, I have resorted to omitting the buffer wash steps and using 
distilled water only. The slide surface remains water repellent and the added 
IHC reagents form a pool over the tissue sections. The detection method is a 
polymer-HRP and there is no increase in background staining in the tissue or on 
the slide surface.

I am assuming that the IHC reagents are prepared in the optimum suspension 
liquid and a buffer is not required. So, I am interested in hearing from anyone 
why this is a bad idea.

Thanks,

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[Histonet] (no subject)

[Travis Troyer]

We are needing to dispose of patient slides and blocks that are beyond the 
years that we need to keep them.What have people found is the safest and 
most economical way to do this?

Thanks,
Travis Troyer
Histology Supervisor
Peterson Laboratory Services
Manhattan, KS
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[Histonet] (no subject)

[Richard Yeo]

   Hey Histonet,
   Can  any  one  out  there on histonet tell me the salary r= ange for a
   grossing histologist?
   And also the regions that these s= alries are in?
   Thanks
   Richard Yeo ryeo@foxmail.wchos= p.org
   Histology Manager
   Wooster Community Hospital
   1761 Bea= ll Ave.
   Wooster Ohio 44691
   330 263 8563
   [1]r...@foxmail.wshosp.org

References

   1. 3Dmailto:ryeo___
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[Histonet] (no subject)

[Renee H. Workman]

Sorry everyone, how long due most labs retain unstained glass slides?  I have 
the CAP requirement but due they have requirements for unstained glass slides.
Renee H. Workman
Histology Supervisor
Virginia Urology
9105 Stony Point Drive
Richmond, VA  23235
W: 804-527-1316 | F: 804-270-0917
rhwork...@uro.commailto:rhwork...@uro.com | www.uro.comhttp://www.uro.com





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Re: [Histonet] (no subject)

[Cristi Stephenson]

I second Toni's suggestion.  Definitely sounds like the paraffin is still
present on the lower sections of the slide.

On Wed, Dec 4, 2013 at 10:17 AM, Rathborne, Toni 
trathbo...@somerset-healthcare.com wrote:

 Could it be the heating/deparaffinization process? If the upper sections
 are staining more evenly, then maybe they are free from residual paraffin.
 Try extending the time in the ovens and/or xylene.

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie J.
 Sent: Wednesday, December 04, 2013 1:12 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] (no subject)

 Hello all,

 I am looking for suggestions on issues with our HE stain.

 I supervised a Veterinary Diagnostic lab for over 27 years and produced
 top quality sections, HE's, special stains and IHC  on a variety of
 different species in our lab.
  I am currently working in a  Dermatopathology Lab and I am  finding
 inconsistent staining with our HE's. Working with just skin is a challenge
 all on its own.  We have made changes to our staining protocol and just not
 happy with the end product.
 What we are observing is inconsistent  staining on levels on the same
 slide.  The top section seems to stain more evenly than the middle and
 bottom sections.  I can actually see three different shades of color.   The
 specimens are ribboned sections so I know it is not from thick and thin
 sections.   We have looked at our processing times, microwave vs. oven
 times, staining reagents, different brands of hematoxylin and eosin,
 adjustments on staining times, tap water compared to distilled water.

 Our main processor is the Thermo Scientific STP-420 and our back up is the
  Sakura VIP V processor.I have been working with Thermo technical
 support thinking it might be a processing issue.  We have a Leica ST5020
 Multstainer/CV5030 Robotic Cover slipper we have made several changes that
 the technical teams has suggested to the reagents and staining time.  It's
 still not the quality that we are looking for.
 I have had culligan techs out several times to see if it could be
 something with  the water.

 We can run 100 slides the same day, same reagents and protocol and the HE
 color is so inconsistent.
  I would appreciate any suggestions in this matter.


 Cherie Chapman, BS, HT, HTL (ASCP)
 Associate Director of Dermatopathology Laboratory University of Missouri
 Department of Dermatology University Physicians Medical Building
 Phone: (573) 884-0123
 Fax: (573) 884-0834

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RE: [Histonet] (no subject)

[Truscott, Tom]

Hi Cherie, Since the top section is consistently better, then the possibilities 
lie in what happens after they are on the slide. As Toni recommends slides that 
are heated or deparaffinized vertically may have left over paraffin on the 
lower regions.Maybe you could also try depar with them up on their long edges. 
Staining slides vertically might also give this appearance if reagent times and 
rinsing are too short. Staining slides flat would point to slides not level or 
reagents not evenly dispersed on the slide. I would not rule out thick-thin on 
ribbons but that is probably not the case since the good section is always at 
the top. Good luck, Tom T

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni
Sent: Wednesday, December 04, 2013 10:17 AM
To: 'Chapman, Cherie J.'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)

Could it be the heating/deparaffinization process? If the upper sections are 
staining more evenly, then maybe they are free from residual paraffin. Try 
extending the time in the ovens and/or xylene.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chapman, Cherie 
J.
Sent: Wednesday, December 04, 2013 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

Hello all,

I am looking for suggestions on issues with our HE stain.

I supervised a Veterinary Diagnostic lab for over 27 years and produced top 
quality sections, HE's, special stains and IHC  on a variety of different 
species in our lab.
 I am currently working in a  Dermatopathology Lab and I am  finding 
inconsistent staining with our HE's. Working with just skin is a challenge all 
on its own.  We have made changes to our staining protocol and just not happy 
with the end product.
What we are observing is inconsistent  staining on levels on the same slide.  
The top section seems to stain more evenly than the middle and bottom sections. 
 I can actually see three different shades of color.   The specimens are 
ribboned sections so I know it is not from thick and thin sections.   We have 
looked at our processing times, microwave vs. oven times, staining reagents, 
different brands of hematoxylin and eosin, adjustments on staining times, tap 
water compared to distilled water.

Our main processor is the Thermo Scientific STP-420 and our back up is the  
Sakura VIP V processor.I have been working with Thermo technical support 
thinking it might be a processing issue.  We have a Leica ST5020 
Multstainer/CV5030 Robotic Cover slipper we have made several changes that the 
technical teams has suggested to the reagents and staining time.  It's still 
not the quality that we are looking for.
I have had culligan techs out several times to see if it could be something 
with  the water.

We can run 100 slides the same day, same reagents and protocol and the HE 
color is so inconsistent.
 I would appreciate any suggestions in this matter.


Cherie Chapman, BS, HT, HTL (ASCP)
Associate Director of Dermatopathology Laboratory University of Missouri 
Department of Dermatology University Physicians Medical Building
Phone: (573) 884-0123
Fax: (573) 884-0834

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[Histonet] (no subject)

[Chapman, Cherie J.]

Thank you for all the suggestions.  Nothing more frustrating when you can't 
point your finger to the exact problem.

Thanks again,

Cherie Chapman, BS, HT, HTL (ASCP)
Associate Director of Dermatopathology Laboratory
University of Missouri Department of Dermatology
University Physicians Medical Building
Phone: (573) 884-0123
Fax: (573) 884-0834

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[Histonet] (no subject)

[Amos Brooks]

Hi,
There are a few ways of doing this. Perhaps the easiest  is scraping the 
cells off while it is still in the media. Transfer it to a centrifuge tube and 
spin it down. Pour off the supernatant add formalism vortex it and centrifuge 
it again and pour off the supernatant again and add Histogel or agar. Then 
process as usual. This is about as close as you are going to get to treating it 
like normal tissue.

Amos


Message: 4 Date: Mon, 2 Dec 2013 17:06:24 + From: Mike Tighe 
mti...@trudeauinstitute.org Subject: [Histonet] Embedding cells in Paraffin 
To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu) 
histonet@lists.utsouthwestern.edu Message-ID: 
46d5589df0864870848089e6780fb...@by2pr07mb469.namprd07.prod.outlook.com

Content-Type: text/plain; charset=us-ascii

Has anyone tried to embed cells grown in tissue culture? I am trying to put 
some tissue culture cells through same stress as tissue would go through. 
Fixation, dehydration, and heat. Any ideas? I could re-suspend in OCT and then 
fix for extended time with NBF but that doesn't quite seem fair.

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[Histonet] (no subject)

[Leann M. Murphy]

I was just wondering if anyone was having difficulty cutting biopsies?  We have 
been using the same blades for years and now it is so difficult to get a 
ribbon.  There are three other area hospitals having the same problem. I have 
been trying samples from various vendors and the problem is still the same.  
Maybe the company that makes these microtome blades have cut costs and is now 
using a lower grade of metal for the blade.  I don't  know what it is, but it 
is driving everyone crazy.  Also, we are spending more money on blades because 
they do not last as long and of course this does not make our Manager happy.  I 
am just very frustrated.  Any suggestions?

LeAnn Murph, HT (ASCP)
Aultman Hosptial
Canton, Ohio
Technical Specialist
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RE: [Histonet] (no subject)

[Rathborne, Toni]

I wouldn't be too quick to blame the blades. Have you changed paraffin 
brands/temperatures or changed any of your processing schedules? Is the 
fixative/fixation process the same? Is the collecting location doing anything 
different/have they had a change in staff? 
Also, what type of blades are you using? It would be easier for people to 
respond if they had this information.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy
Sent: Thursday, November 14, 2013 11:04 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)

I was just wondering if anyone was having difficulty cutting biopsies?  We have 
been using the same blades for years and now it is so difficult to get a 
ribbon.  There are three other area hospitals having the same problem. I have 
been trying samples from various vendors and the problem is still the same.  
Maybe the company that makes these microtome blades have cut costs and is now 
using a lower grade of metal for the blade.  I don't  know what it is, but it 
is driving everyone crazy.  Also, we are spending more money on blades because 
they do not last as long and of course this does not make our Manager happy.  I 
am just very frustrated.  Any suggestions?

LeAnn Murph, HT (ASCP)
Aultman Hosptial
Canton, Ohio
Technical Specialist
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[Histonet] (no subject)

[Richard Yeo]

   I  have  an  abundance  of fungus controls. I need afb and h.pylori. I
   would be= more than happy to trade. If you need some other control let
   me know an= d I'll see if I can help
   Thanks
   Richard Yeo ryeo@foxma= il.wchosp.org
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