Re: [Histonet] [EXTERNAL]TEST

[Piche, Jessica via Histonet]

Hi Terri,

I see your message. 

Have a good day.

Jessica


Jessica Piché, HT(ASCP)

Histology Team Leader, Laboratory

Waterbury Health
64 Robbins Street

Waterbury, Connecticut, 06708

Phone: 203-573-7167
FAX: 203-573-7242

Email:jessica.pi...@wtbyhosp.org

Waterbury HEALTH


From: Terri Braud via Histonet 
Sent: Monday, July 15, 2024 10:13 AM
To: histonet@lists.utsouthwestern.edu 
Subject: Re: [Histonet] [EXTERNAL]TEST

[EXTERNAL MSG]

This is a test to see if I can post to the list again.

Terri L. Braud, HT(ASCP)
HNL Laboratories for
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-2021
  Honesty
AccouNtability
AgiLity
CoLlaboration
  CoMpassion


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Re: [Histonet] [EXTERNAL]TEST

[Terri Braud via Histonet]

This is a test to see if I can post to the list again.

Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-2021
  Honesty
AccouNtability
    AgiLity
    CoLlaboration
  CoMpassion


***Please Note: Redeemer Health has now changed its email domain from 
@holyredeemer . com to @redeemerhealth . org. Please alert your 
IT/cybersecurity team to ensure our new email domain is safe-listed.***

This email, and any files transmitted with it, are confidential and intended 
solely for the use of the individual or entity to whom they are addressed. The 
information contained in this transmission may contain privileged and 
confidential information, including patient information protected by federal 
and state privacy laws. If you are not the intended recipient, please telephone 
or email the sender and delete this message and any attachment from your 
system; you must not copy or disclose the contents of this message or any 
attachment to any other person. Any views expressed in this message are those 
of the individual sender, except where the sender specifies and with authority, 
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Re: [Histonet] [External] Re: Agarose embedded tissue arrays embedded in paraffin block

[Otto, Christopher M via Histonet]

Hi Colleen,

they are blocks of spheroids so they are pretty small.  I don't know how they 
were processed or how the agarose was prepared.

The samples you are talking about, that you ran a short run on, how did that 
behave when you tried to section on the microtome?

Thanks
-Chris

From: Colleen Forster 
Sent: Friday, May 17, 2024 3:06 PM
To: Jay Lundgren 
Cc: Otto, Christopher M ; 
histonet@lists.utsouthwestern.edu 
Subject: [External] Re: [Histonet] Agarose embedded tissue arrays embedded in 
paraffin block

You don't often get email from cfors...@umn.edu. Learn why this is 
important
Chistopher,

Were these small pieces of tissue? If they are in an agar (such as histogel) 
they would need to have been processed overnight to ensure the agar is 
completely dehydrated during processing. The sample, no matter how small, is 
protected and processes perfectly. IF you ran a short run with the agar it will 
not have [processed properly. I was never able to salvage those samples. It 
took me a couple times to figure out the solution.

Just a thought.

Colleen Forster HT(ASCP)QIHC

On Fri, May 17, 2024 at 2:32 PM Jay Lundgren via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:
Where did the agarose come from?

On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet <
histonet@lists.utsouthwestern.edu> 
wrote:

>
>
> Hello everyone!
>
>  I'm having trouble sectioning tissue array blocks where the array is in
> agarose embedded into a paraffin block.  I've chilled the blocks and I'm
> sectioning on a rotary microtome, at 5 microns, with a high profile
> Accuedge blade. The paraffin surrounding the agarose sections normally, but
> the agarose portion of the block causes the blade to "skip" across it
> slightly and even chip out as if my blade isn't snug in the blade holder
> (it is).  If I do get a tiny portion of agarose on my section to float out
> on the waterbath it flies away (like adding ETOH to a waterbath with
> sections already on it.)   Everyone I have asked about this says the
> agarose should section normally with the paraffin like any other FFPE
> blocks. Any ideas on why this agarose is behaving this way for me?  Thank
> you in advance!
>
>
>
> ___
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--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930






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Re: [Histonet] [External] Re: Agarose embedded tissue arrays embedded in paraffin block

[Otto, Christopher M via Histonet]

I didn't take part in the preparation of the agarose or the processing. Was 
pretty much just handed the blocks and asked to make slides.

Get Outlook for Android

From: Jay Lundgren 
Sent: Friday, May 17, 2024 2:31:44 PM
To: Otto, Christopher M 
Cc: histonet@lists.utsouthwestern.edu 
Subject: [External] Re: [Histonet] Agarose embedded tissue arrays embedded in 
paraffin block

You don't often get email from jaylundg...@gmail.com. Learn why this is 
important
Where did the agarose come from?

On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:


Hello everyone!

 I'm having trouble sectioning tissue array blocks where the array is in 
agarose embedded into a paraffin block.  I've chilled the blocks and I'm 
sectioning on a rotary microtome, at 5 microns, with a high profile Accuedge 
blade. The paraffin surrounding the agarose sections normally, but the agarose 
portion of the block causes the blade to "skip" across it slightly and even 
chip out as if my blade isn't snug in the blade holder (it is).  If I do get a 
tiny portion of agarose on my section to float out on the waterbath it flies 
away (like adding ETOH to a waterbath with sections already on it.)   Everyone 
I have asked about this says the agarose should section normally with the 
paraffin like any other FFPE blocks. Any ideas on why this agarose is behaving 
this way for me?  Thank you in advance!



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Re: [Histonet] [EXTERNAL] Histonet Digest, Vol 246, Issue 7

[Mayer,Toysha N via Histonet]

Jay,

I would think that the alkaline properties of the ammonia slow the swelling of 
the tissues unlike plain water.

Sincerely,

Toysha N. Mayer, DHSc, MBA, HT (ASCP)
Asst Professor/ Assoc Program Director
HTL Program
UTMDACC
tnma...@mdanderson.edu
off cell: 832-710-1837
off: 713-563-3481

* My workday may look different from your workday. Please do not feel obligated 
to respond outside of your normal working hours.




Today's Topics:

   1. Re: Inquiry on Tissue Softening for Microtomy (Jay Lundgren)
   2. Re: Inquiry on Tissue Softening for Microtomy (John Kiernan)


--

Message: 1
Date: Sat, 11 May 2024 15:40:41 -0500
From: Jay Lundgren 
To: John Kiernan 
Cc: "histonet@lists.utsouthwestern.edu"
,  IGNACIO GONZ?LEZ MASSONI

Subject: Re: [Histonet] Inquiry on Tissue Softening for Microtomy
Message-ID:

Content-Type: text/plain; charset="UTF-8"

I wouldn't say softening, I would say hydrating.  Ammonia water accelerates 
hydration of FFPE blocks.  Nobody knows how it works, it's a mystery.  Or at 
least I've never heard a scientific explanation.  Only pseudo-scientific mumbo 
jumbo like "facilitates the removal of paraffin" which is false.  Go soak a 
solid block of paraffin in pure NH4OH for 24 hours, it won't do anything.

I was told as a student at AFIP, "It opens the pores of the tissue so water can 
get in."  In other words, pseudo-scientific mumbo jumbo.

 It works though.  Somebody needs to get a $150,000,000 NIH grant and do a 
research project on how ammonia water hydrates tissue.  I worked with some 
lovely Hmong people in California that called it "crying water".

Using it can cause a big interpersonal problem with certain people in the lab 
though.  Interestingly, hypersensitivity to smells is one of the symptoms of 
autism.  Hypersensitivity to smells is also highly correlated with bipolar 
disorder and heightened emotional reactivity.  Sooo...

Jay A. Lundgren, M.S., HTL (ASCP)

On Fri, May 10, 2024 at 11:14?PM John Kiernan via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

>If you apply the ammonia to the cut surface of the paraffin block,
> I suspect that it softens the tissue in the same way as applying
> water: by entering interstices of the tissue that are not occupied by
> paraffin molecules.
>I never tried ammonia for this purpose but in the 1960s to early
> '70s I occasionally used a proprietary product called Mollifex, which
> I see is still sold. In 1972 or '73 an elderly technician told me that
> water was just as good, and I soon found out that he was right.
> Indeed, water had the advantage of working in 15-30 minutes rather than 
> taking several hours.
> John Kiernan
> Emeritus, UWO, London, Canada
> https://urldefense.com/v3/__https://publish.uwo.ca/*jkiernan/__;fg!!Pf
> beBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1f7YbeM8x7BKiM3NXEejTAY
> jEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeH0EH0M$
> = = =
> 
> From: IGNACIO GONZ?LEZ MASSONI via Histonet <
> histonet@lists.utsouthwestern.edu>
> Sent: May 10, 2024 8:53 PM
> To: histonet@lists.utsouthwestern.edu
> 
> Subject: [Histonet] Inquiry on Tissue Softening for Microtomy
>
> Dear friends at Histonet,
>
> I hope this message finds you well. I am reaching out to seek your
> expertise on a matter that has piqued my interest in the field of histology.
>
> I am currently delving into the process of preparing FFPE
> (formalin-fixed,
> paraffin-embedded) tissues for microtomy. Specifically, I am curious
> about the role of ammonia in softening these tissues before
> sectioning. From my understanding, ammonia serves as an alkaline agent
> that helps neutralize formalin's effects and facilitates the removal
> of paraffin, thus aiding in the softening of the tissue.
>
> However, I would greatly appreciate if you could provide a more
> detailed explanation of the chemical interactions at play here. How
> exactly does ammonia interact with the tissue components to achieve
> the desired softening effect? Moreover, are there any best practices
> or safety precautions that one should be aware of when using ammonia in this 
> context?
>
> Your insights on this topic would be invaluable to me and would
> greatly enhance my understanding of the intricacies involved in
> histological preparations.
>
> Thank you for your time and assistance.
>
> Warm regards from Santiago of Chile
>
> Ignacio, Medical Technologist
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> stinfo/histonet__;!!PfbeBCCAmug!juk_i8Jg3ONUBsR51Umi3igC1SLDdMxRkkmmp1
> f7YbeM8x7BKiM3NXEejTAYjEhu2b6T7wVQbTbUwRXPM0rOXYult8zZEn_W5G2JeTLNjoU$
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> 

Re: [Histonet] [EXTERNAL] Epredia xylene substitute

[Ann Specian via Histonet]

 Thank you!
On Sunday, April 28, 2024 at 02:19:24 AM EDT, Gudrun Lang  
wrote:  
 
 Hi Ann,
we have used ShellSol for years in our VIPs until our supplier stopped it. 
That's also a kind of naphta with aliphatic hydrogencarbons.
After that wie changed to the Sakura-substitute with the same protocol. For 
standard processing we had/have two stations with 60 min each at 40 °C. For fat 
processing the time is doubled.
That works for our specimens well enough.
Gudrun

-Ursprüngliche Nachricht-
Von: Ann Specian via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Freitag, 26. April 2024 02:07
An: Will Cavett
Cc: Histonet
Betreff: Re: [Histonet] [EXTERNAL] Epredia xylene substitute

Hi Will, great to hear from you!Yes those are all the reasons why we are 
considering it, but we would like to start validating tissue and rather than 
reinvent the wheel, We are looking for Processing protocols that have already 
been tried and true. If you have any,  if you could share them, it would be 
greatly appreciated.


Sent from the all new AOL app for iOS


On Thursday, April 25, 2024, 6:39 PM, Will Cavett  wrote:

I hope you are doing well, Ann? We considered Epredia's because it seems to 
offer a safer and more convenient option than xylene. Also, the evaporation 
rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring 
consistent performance in tissue processing .And, unlike xylene, which has a 
strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its 
airborne exposure limit is three times safer than xylene. Hope this helps.

Will Cavett, II

-Original Message-
From: Ann Specian via Histonet 
Sent: Wednesday, April 24, 2024 2:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL][Histonet] Epredia xylene substitute

We are thinking of changing from xylene to this substitute. Does anybody have 
any Processing protocols using Epredia Xylene substitute that they could share?


Sent from the all new AOL app for iOS
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Re: [Histonet] [EXTERNAL] Epredia xylene substitute

[Gudrun Lang via Histonet]

Hi Ann,
we have used ShellSol for years in our VIPs until our supplier stopped it. 
That's also a kind of naphta with aliphatic hydrogencarbons.
After that wie changed to the Sakura-substitute with the same protocol. For 
standard processing we had/have two stations with 60 min each at 40 °C. For fat 
processing the time is doubled.
That works for our specimens well enough.
Gudrun

-Ursprüngliche Nachricht-
Von: Ann Specian via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Gesendet: Freitag, 26. April 2024 02:07
An: Will Cavett
Cc: Histonet
Betreff: Re: [Histonet] [EXTERNAL] Epredia xylene substitute

Hi Will, great to hear from you!Yes those are all the reasons why we are 
considering it, but we would like to start validating tissue and rather than 
reinvent the wheel, We are looking for Processing protocols that have already 
been tried and true. If you have any,  if you could share them, it would be 
greatly appreciated.


Sent from the all new AOL app for iOS


On Thursday, April 25, 2024, 6:39 PM, Will Cavett  wrote:

I hope you are doing well, Ann? We considered Epredia's because it seems to 
offer a safer and more convenient option than xylene. Also, the evaporation 
rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring 
consistent performance in tissue processing .And, unlike xylene, which has a 
strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its 
airborne exposure limit is three times safer than xylene. Hope this helps.

Will Cavett, II

-Original Message-
From: Ann Specian via Histonet 
Sent: Wednesday, April 24, 2024 2:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL][Histonet] Epredia xylene substitute

We are thinking of changing from xylene to this substitute. Does anybody have 
any Processing protocols using Epredia Xylene substitute that they could share?


Sent from the all new AOL app for iOS
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Re: [Histonet] [EXTERNAL] Epredia xylene substitute

[Ann Specian via Histonet]

Hi Will, great to hear from you!Yes those are all the reasons why we are 
considering it, but we would like to start validating tissue and rather than 
reinvent the wheel, We are looking for Processing protocols that have already 
been tried and true. If you have any,  if you could share them, it would be 
greatly appreciated.


Sent from the all new AOL app for iOS


On Thursday, April 25, 2024, 6:39 PM, Will Cavett  wrote:

I hope you are doing well, Ann? We considered Epredia's because it seems to 
offer a safer and more convenient option than xylene. Also, the evaporation 
rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring 
consistent performance in tissue processing .And, unlike xylene, which has a 
strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its 
airborne exposure limit is three times safer than xylene. Hope this helps.

Will Cavett, II

-Original Message-
From: Ann Specian via Histonet 
Sent: Wednesday, April 24, 2024 2:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL][Histonet] Epredia xylene substitute

We are thinking of changing from xylene to this substitute. Does anybody have 
any Processing protocols using Epredia Xylene substitute that they could share?


Sent from the all new AOL app for iOS
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Re: [Histonet] [EXTERNAL] Epredia xylene substitute

[Will Cavett via Histonet]

I hope you are doing well, Ann? We considered Epredia's because it seems to 
offer a safer and more convenient option than xylene. Also, the evaporation 
rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring 
consistent performance in tissue processing .And, unlike xylene, which has a 
strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its 
airborne exposure limit is three times safer than xylene. Hope this helps.

Will Cavett, II

-Original Message-
From: Ann Specian via Histonet 
Sent: Wednesday, April 24, 2024 2:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL][Histonet] Epredia xylene substitute

We are thinking of changing from xylene to this substitute. Does anybody have 
any Processing protocols using Epredia Xylene substitute that they could share?


Sent from the all new AOL app for iOS
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Re: [Histonet] [EXTERNAL]Re: Premaking slides

[Will Cavett via Histonet]

Hello Karen:

At my institution, which is a teaching facility and a level one trauma center, 
these are scheduled. The pathologists felt this was best for patient safety. 
The slides are labeled per procedure and not labeled before so we don’t have a 
lot of slides labeled. We have trained the staff collecting to inform us of 
location change when they come out with the sample. This helps our staff 
tremendously. I hope this helps.

Will Cavett, II


-Original Message-
From: Jay Lundgren via Histonet 
Sent: Thursday, April 25, 2024 4:16 PM
To: Karen Schieberl CA-San Francisco 
Cc: Histonet 
Subject: [EXTERNAL]Re: [Histonet] Premaking slides

I mean, you're not going to hand the pathologist an unlabelled slide, obviously.

This leaves you two options, label the slide before the tissue is on it, or 
afterward.  Labelling the slide before is easier and quicker.

I'm gonna label my slides *immediately* before cutting the frozen, or squishing 
the tissue.  Patient name at the very least, if it hasn't been accessioned yet.

But you don't want to leave any labeled blank slides laying around, that's 
asking for trouble.  If you label too many slides, for instance, discard them 
immediately.

If you are doing multiple cases at once, you are doing it wrong.  I know a lot 
of places operate like this, but frozens are supposed to be scheduled for a 
reason.  I mean, you might get an occasional emergency frozen for an ectopic 
pregnancy, but that's rare.

There is a scheduler in surgery, they should be doing their job.  Or maybe the 
head of Pathology needs to talk to the head of Surgery and explain how risky it 
is to be dealing with multiple frozens at once.  Especially if it's the same 
tissue type.

I'll probably get a lot of flak for this viewpoint, "Oh that's impossible at my 
institution!".  But it's not.  I've dealt with this as manager at several 
institutions and it can be fixed.  It's not going to make you any
friends in Surgery, but it's ultimately about patient safety.   It's just
that most hospitals are terrified of surgeons and will let them do whatever 
they want.

How about the surgeon that leaves the building before the pathologist calls the 
results back?  That's my favorite.


Jay A. Lundgren, M.S., HTL (ASCP)


On Thu, Apr 25, 2024 at 3:31 PM Karen Schieberl CA-San Francisco < 
karen.schieb...@commonspirit.org> wrote:

> This does apply to Frozen and Touch preps.
> Karen Schieberl HT ASCP
>
> Lead Histology Technician
>
> St. Mary's Medical Center
>
> 450 Stanyan St.
>
> San Francisco, Ca. 94117
>
> 415-750-5751
>
> karen.schieberl@ commonspirit.org
>
>
>
>
> Caution:  This email message, including all content and attachments,
> is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.
> The information contained in this email message is intended only for
> the use of the recipient(s) named above. If the reader of this message
> is not the intended recipient or an agent responsible for delivering
> it to the intended recipient, you have received this document in
> error.  Any further review, dissemination, distribution, or copying of
> this message is strictly prohibited.  If you have received this
> communication in error, please notify us  immediately by reply email.
> Thank you
>
>
> On Thu, Apr 25, 2024 at 1:20 PM Jay Lundgren 
> wrote:
>
>> *USE CAUTION - EXTERNAL EMAIL*
>> --
>> You mean for frozens?
>>
>> On Thu, Apr 25, 2024 at 12:44 PM Karen Schieberl CA-San Francisco via
>> Histonet  wrote:
>>
>>> Good Morning,
>>> I am not sure if there is a CAP or Joint commission Policy on pre
>>> making slides for intraoperative consultation.  Can someone let me know if 
>>> there
>>> is one?   I have looked but was unable to find one.  It just seems like
>>> errors can be made especially if you have multiple patients or for
>>> some reason the information on the slide does not match the patient.
>>> Any help would be greatly appreciated.
>>>
>>> Thanks,
>>> Karen Schieberl HT ASCP
>>>
>>> Lead Histology Technician
>>>
>>> St. Mary's Medical Center
>>>
>>> 450 Stanyan St.
>>>
>>> San Francisco, Ca. 94117
>>>
>>> 415-750-5751
>>>
>>> karen.schieberl@ commonspirit.org
>>>
>>>
>>>
>>>
>>> Caution:  This email message, including all content and attachments,
>>> is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.
>>> The information contained in this email message is intended only for
>>> the use of the recipient(s) named above. If the reader of this
>>> message is not the intended recipient or an agent responsible for
>>> delivering it to the intended recipient, you have received this
>>> document in error.  Any further review, dissemination, distribution,
>>> or copying of this message is strictly prohibited.  If you have
>>> received this communication in error, please notify us  immediately
>>> by reply email.  Thank you
>>>
>>> Caution: This email is both proprietary and confidential, and not
>>> intended for transmission to (or receipt by) 

Re: [Histonet] [EXTERNAL] Re: [EXT] Reagent alcohols and tissue processors

[Rinker,Jeff via Histonet]

First, I would like to say the Paloris is the best processor out there. This 
answers the second question too, I think. The standard that I have always used 
is 70% but with machines coming out now that are so good at monitoring the 
concertation's of the alcohols it is interesting to watch.

From: Colleen Forster via Histonet 
Sent: Thursday, March 28, 2024 11:55 AM
To: Paula Sicurello 
Cc: HistoNet 
Subject: [EXTERNAL] Re: [Histonet] [EXT] Reagent alcohols and tissue processors

Paula,

I start my processor at 80%. I am in research and often the samples have
been fixed and are put into 70-80% ethanol for holding or transport.
Starting with 80% steps right in line with the samples. I rarely see a
schedule that starts at 50%. However, I do have a couple labs that ask me
for that. I am fortunate to have a second processor I can use for special
programs and not hold up my main runs.

Colleen Forster HT(ASCP)QIHC
University of Minnesota
BLS Histology and IHC Laboratory

On Thu, Mar 28, 2024 at 9:15 AM Paula Sicurello via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Kara,
> I do like the VIPs.   Besides getting rid of the NBF, the alcohols are
> getting rid of the water as well.
> I learned EM first, so my processing thoughts are probably biased by that.
> Paula
>
> Yahoo Mail: Search, Organize, Conquer
>
>   On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip
> wrote:   So for your first one I love the Sakura Tissue-Tek VIP. Easy to
> use, easy maintenance, and very customizable for programed runs.Every lab I
> have been in has always started at 70. It saves the techs extra time mixing
> up dilutions because you can always find RTU 70%. It also helps I have
> never had any issues with the tissues starting at 70. I get the idea of
> starting lower but you are also then going to need to increase your
> processing time which can delay turnaround times.Plus correct me if I am
> wrong but isn't the whole point of the alcohols after NBF to get the NBF
> out of the tissue and ready for xylene and wax?
>
> Phillip Kara, HTL | Senior Research Associate
>
> University of North Texas Health Science Center
>
> Division of Research and Innovation
>
> a: 3500 Camp Bowie Blvd, Fort Worth, TX 76107
>
> p: 918-281-9060
>
> w:http://www.unthsc.edu/corelabs
>
> From: Paula Sicurello via Histonet 
> Sent: Thursday, March 28, 2024 8:04 AM
> To: HistoNet 
> Subject: [EXT] [Histonet] Reagent alcohols and tissue processors Good
> Morning,
> My question two part:
> Best tissue processor ever and why?
> What is percentage is your first alcohol step?
> I see that lots of places start at 70%  but I favor a lower %.  One
> because it's gentler on the tissue, and two because 10% NBF precipitates
> out if you start at 70%.
> Thanks for your insight,
> Paula
>
> Yahoo Mail: Search, Organize, Conquer
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>
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>


--
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930
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Re: [Histonet] [EXTERNAL] Histonet Digest, Vol 238, Issue 13

[Terri Braud via Histonet]

We have something that is a bit of a hybrid that we call a "dummy" requisition. 
 
A person "orders" a "test" for pathology or cytology in the hospital 
information system.  The form they fill out has all of the information that 
would appear on a paper requisition.  When completed, it prints to a printer in 
Pathology and is matched to the specimens in the department (this could print 
to where the specimens are collected).  The printed copy becomes the 
requisition. The pathology dummy test is autoresulted as "see pathology 
report".  The actual accessioning of the specimen into the LIS creates the true 
pathology test with result(report) pending.  
Advantages:  One can make required fields (such as clinical history) so that 
they must be filled out, which captures information required by CAP.
Cons: A pain when something needs to be corrected.  We usually have them cancel 
the order and submit another order.

Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-2021
-Original Message-

Today's Topics:

   1. Electronic requisitions (Jacque Sagasser)
From: Jacque Sagasser 
Subject: [Histonet] Electronic requisitions
Hello
Does anyone use electronic requisitions? If so, would you mind describing your 
system for staying compliant with CLIA? Is a paper requisition sent in addition 
to the electronic requisition? If not, how does that work with accessioning and 
matching the specimens and patient information?  We currently use a paper 
system and the requisitions are filed and kept for 10 years. We would like to 
move to an electronic system, but I am not sure how to implement it, while 
staying compliant and not complicating the process. We do not have a bar code 
system set up. I could scan the paper requestions into the patient file, but 
that would be more work than just continuing to file them. I am also running 
out of room to file the requisitions though, so I am trying to explore my 
options. Thank you in advance for your input.

Jacque R. Sagasser, HT (ASCP)cm
Gandhi GI Pathology, LLC
999 Brubaker Drive
Suite 1
Kettering OH 45429
jsagas...@gandhigi.com
937-795-1099 phone
937-519-1321 fax


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Re: [Histonet] [External] Expiration date after opeing a reagent

[Rachel Gonzalez via Histonet]

Hi

I agree the inspector is wrong.

I work in the manufacture of pathology reagents such as secondary polymers, 
diluents, buffers, antibodies, IF conjugates. We constantly test in real time 
to make sure that the product is as good on its expiration date as it is on day 
one. We are mostly for RUO,  I am sure that the larger companies such as 
Ventana Dako and Leica are doing the same. 

To be fair to the inspector maybe he is confusing antibodies that should be 
kept at -20C and are stored at 4C. Those antibodies will have a shorter 
half-life. We state this on our inserts with these antibodies.  

Rachel
-Original Message-
From: Thomas Podawiltz via Histonet  
Sent: Friday, July 21, 2023 5:30 PM
To: Bitting, Angela K. ; Paula ; 
histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] [External] Expiration date after opeing a reagent

I think that inspector has their head up their……….


Sent from Yahoo Mail for iPad


On Friday, July 21, 2023, 4:45 PM, Bitting, Angela K. via Histonet 
 wrote:

This sounds absurd to me.

-Original Message-
From: Paula via Histonet 
Sent: Friday, July 21, 2023 4:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Expiration date after opeing a reagent

External: Be careful with links and files. If suspicious, alert the Information 
Security Office. Questions? Call 570-271-8092


Hello,



We just got CLIA inspected and had no deficiencies. The inspector did say he 
wants to see us write down on any reagent containers when it was opened, and 
write down the new expiration date.



The containers have the expiration date on them from the manufacturer, but the 
inspector said when the container is opened, the expiration will change and we 
need to write the date on the container under the opened date.



I've never heard of this requirement and I would like to ask if you have.
He said if the vendor does not give us any written documentation of when a 
reagent would expire after it's opened, then we should seek an alternate vendor 
who can.



Any comments, etc. are greatly appreciated.



Paula Lucas

Bio-Path Medical Group



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Re: [Histonet] [External] Expiration date after opeing a reagent

[Thomas Podawiltz via Histonet]

I think that inspector has their head up their……….


Sent from Yahoo Mail for iPad


On Friday, July 21, 2023, 4:45 PM, Bitting, Angela K. via Histonet 
 wrote:

This sounds absurd to me.

-Original Message-
From: Paula via Histonet 
Sent: Friday, July 21, 2023 4:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Expiration date after opeing a reagent

External: Be careful with links and files. If suspicious, alert the Information 
Security Office. Questions? Call 570-271-8092


Hello,



We just got CLIA inspected and had no deficiencies. The inspector did say he 
wants to see us write down on any reagent containers when it was opened, and 
write down the new expiration date.



The containers have the expiration date on them from the manufacturer, but the 
inspector said when the container is opened, the expiration will change and we 
need to write the date on the container under the opened date.



I've never heard of this requirement and I would like to ask if you have.
He said if the vendor does not give us any written documentation of when a 
reagent would expire after it's opened, then we should seek an alternate vendor 
who can.



Any comments, etc. are greatly appreciated.



Paula Lucas

Bio-Path Medical Group



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prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
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Re: [Histonet] [External] Expiration date after opeing a reagent

[Bitting, Angela K. via Histonet]

This sounds absurd to me.

-Original Message-
From: Paula via Histonet 
Sent: Friday, July 21, 2023 4:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Expiration date after opeing a reagent

External: Be careful with links and files. If suspicious, alert the Information 
Security Office. Questions? Call 570-271-8092


Hello,



We just got CLIA inspected and had no deficiencies. The inspector did say he 
wants to see us write down on any reagent containers when it was opened, and 
write down the new expiration date.



The containers have the expiration date on them from the manufacturer, but the 
inspector said when the container is opened, the expiration will change and we 
need to write the date on the container under the opened date.



I've never heard of this requirement and I would like to ask if you have.
He said if the vendor does not give us any written documentation of when a 
reagent would expire after it's opened, then we should seek an alternate vendor 
who can.



Any comments, etc. are greatly appreciated.



Paula Lucas

Bio-Path Medical Group



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prohibited and may be unlawful. If you have received this message in error, 
please delete all electronic copies of this message (and the documents attached 
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encrypted, the recipient will receive an e-mail instructing them to sign on to 
the Geisinger Health System Secure E-mail Message Center to retrieve the 
encrypted
  e-mail.

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Re: [Histonet] [External] unprocessed tissue and Excelsior AS issue

[Alonso Martínez Canabal via Histonet]

Good morning,
I want to perform immunohistochemistry in mouse postnatal brains of
0,5,10 days. I have seen that many people, including Paxinos, make the
sections including the skull. Do you think that I should follow this using
a protocol for decalcification of the skull?
Thank you!

El vie, 30 jun 2023 a las 13:21, Dessasau, Evan D. via Histonet (<
histonet@lists.utsouthwestern.edu>) escribió:

> Hi Mr. Horvath, I work at a Biomedical research facility with non-human
> primates as the main lab animal.  If you could use wet tissue from
> non-human primates, you could submit a Specimen Request for access to
> tissue for your project.  If interested, please email Mrs. Summerville
> cc'ed here.
> E-van
>
>
>
> -Original Message-
> From: Andrew Horvath via Histonet 
> Sent: Friday, June 30, 2023 2:41 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [External] [Histonet] unprocessed tissue and Excelsior AS issue
>
> Hello all,
>
>
>
> I have two things to address in this email.
>
>
>
> First, I am trying to locate tissue that has not been processed so we can
> use it for testing of our tissue processors.  Optimally it would be colon
> but also esophagus as we are a GI lab.  Does anyone know where I can find
> such tissue (in the US preferably)?
>
>
>
> Second, we use the Epredia Excelsior AS processor and have run into issues
> with the processing of tissue where portions of small biopsy specimens will
> have sections that appear to not be processed.  It does not happen on every
> specimen but is consistent enough we are trying to find solutions to this
> problem.  Hence the non-processed tissue I am trying to locate.
>
>
>
> Any help is appreciated!
>
>
>
>
>
>
>
> Thanks,
>
>
>
> Andrew
>
>
>
>
>
> Andrew Horvath, MBA, MA
>
> Business Operations Manager
>
> 303-220-4369
>
>
>
> Centennial Pathologists, PC
>
> 7346 South Alton Way, Suite 10-D
>
> Centennial, CO 80112
>
>
>
>
>
> Confidentiality Notice:
>
> This message (including any attachments) is intended only for the use of
> the individual or entity to which it is addressed and may contain
> information that is non-public, proprietary, privileged, or confidential.
> If you are not the intended recipient, you are hereby notified that any
> use, dissemination, distribution, or copying of this communication is
> strictly prohibited.  If you have received this communication in error,
> notify us immediately by telephone and (i) destroy this message if a
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-- 
Dr. Alonso Martínez Canabal PhD
Profesor Asociado "C"
Departamento de Biología Celular, Facultad de Ciencias, UNAM
Investigador Nacional "I"
56224833
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Re: [Histonet] [External] unprocessed tissue and Excelsior AS issue

[Dessasau, Evan D. via Histonet]

Hi Mr. Horvath, I work at a Biomedical research facility with non-human 
primates as the main lab animal.  If you could use wet tissue from non-human 
primates, you could submit a Specimen Request for access to tissue for your 
project.  If interested, please email Mrs. Summerville cc'ed here.
E-van



-Original Message-
From: Andrew Horvath via Histonet 
Sent: Friday, June 30, 2023 2:41 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] unprocessed tissue and Excelsior AS issue

Hello all,



I have two things to address in this email.



First, I am trying to locate tissue that has not been processed so we can use 
it for testing of our tissue processors.  Optimally it would be colon but also 
esophagus as we are a GI lab.  Does anyone know where I can find such tissue 
(in the US preferably)?



Second, we use the Epredia Excelsior AS processor and have run into issues with 
the processing of tissue where portions of small biopsy specimens will have 
sections that appear to not be processed.  It does not happen on every specimen 
but is consistent enough we are trying to find solutions to this problem.  
Hence the non-processed tissue I am trying to locate.



Any help is appreciated!







Thanks,



Andrew





Andrew Horvath, MBA, MA

Business Operations Manager

303-220-4369



Centennial Pathologists, PC

7346 South Alton Way, Suite 10-D

Centennial, CO 80112





Confidentiality Notice:

This message (including any attachments) is intended only for the use of the 
individual or entity to which it is addressed and may contain information that 
is non-public, proprietary, privileged, or confidential.  If you are not the 
intended recipient, you are hereby notified that any use, dissemination, 
distribution, or copying of this communication is strictly prohibited.  If you 
have received this communication in error, notify us immediately by telephone 
and (i) destroy this message if a facsimile or (ii) delete this message 
immediately if this is an electronic communication



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Re: [Histonet] [External] Re: Coffee at the desk

[Akemi Allison via Histonet]

  15:4-Observing Standard Precautions

   Occupational Safety and Health Administration (OSHA)

   Can a health care worker drink coffee in a laboratory where blood/body
   fluids/tissue tests are performed? Why or Why not?

   No, federal law prohibits eating and drinking in any area that can be
   potentially contaminated by blood or other body fluids.

   Regards,

   Akemi Allison-Tacha, BS, HT/HTL ASCP

   President/Consultant

   Phoenix Laboratory Consulting

   Sent from my iPhone

 On Jun 2, 2023, at 12:37 PM, Bitting, Angela K. via Histonet
  wrote:

   Oh, for the days when one could tuck a 32 oz. Slurpy inconspicuously
   behind the VIP without fear of admonishment.
   -Original Message-
   From: Jay Lundgren via Histonet 
   Sent: Friday, June 2, 2023 3:34 PM
   To: Piche, Jessica 
   Cc: Histonet@lists.utsouthwestern.edu
   Subject: [External] Re: [Histonet] Coffee at the desk
   External: Be careful with links and files. If suspicious, alert the
   Information Security Office. Questions? Call 570-271-8092
   Next thing you're going to be telling me it's not OK to embed with a 1
   foot tall bunsen burner open flame and a Coke on the cold tray!
   On Fri, Jun 2, 2023 at 7:46 AM Piche, Jessica via Histonet <
   histonet@lists.utsouthwestern.edu> wrote:

 It also doesn't set a good impression for everyone else in the lab

 since they can't have a coffee at their stations. If it could be
 done,

 I wouldn't want everyone else's coffees on my desk either,  but I'd

 have to because I'd want to make it fair to everyone. If only it was
 safe for all of us

 Have a great weekend everyone!

 Jessica

 Jessica Piche, HT(ASCP)

 Waterbury Hospital Histology Laboratory Histology Team Leader

 203-573-7167

 

 From: McLaughlin, Stacy L. via Histonet

 ;

 Histonet@lists.utsouthwestern.edu
 

 Subject: Re: [Histonet] Coffee at the desk

 [EXTERNAL MSG]

 It is not allowed.  The area you want food/drink has to be
 completely

 separate- floor to ceiling walls and a door.

 Stacy McLaughlin, HT(ASCP) QLScm

 Histology Supervisor

 Cooley Dickinson Hospital

 30 Locust Street

 Northampton, MA 01060

 Office:  (413)582-2019

 Lab:  (413)582-2179

 smclaughl...@cooleydickinson.org

 -Original Message-

 From: Hannen, Valerie via Histonet
 

 Sent: Thursday, June 1, 2023 3:40 PM

 To: Histonet@lists.utsouthwestern.edu

 Subject: [Histonet] Coffee at the desk

External Email - Use Caution

 Is there any regulation/ reason why a Histology supervisor whose
 desk

 is in the department and the desk area is taped off and designated
 as

 a "clean area (  meaning no chemicals or specimens cross the taped
 off

 area) can't have a closed coffee cup at their desk??  We are having
 a

 debate in our Lab and I wanted to get a consensus.

 Thanks in Advance,

 Valerie

 Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief
 Parrish

 Medical Center

 951 N. Washington Avenue

 Titusville, Florida 32796

 P: 321-268-6333  Ext. 7506

 F: 321-268-6149

 valerie.han...@parrishmed.com

 http://secur/

 e-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr
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 Tc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__w
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 bG

 Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn
 0%

Re: [Histonet] [External] Re: Coffee at the desk

[Bitting, Angela K. via Histonet]

Oh, for the days when one could tuck a 32 oz. Slurpy inconspicuously behind the 
VIP without fear of admonishment.

-Original Message-
From: Jay Lundgren via Histonet 
Sent: Friday, June 2, 2023 3:34 PM
To: Piche, Jessica 
Cc: Histonet@lists.utsouthwestern.edu
Subject: [External] Re: [Histonet] Coffee at the desk

External: Be careful with links and files. If suspicious, alert the Information 
Security Office. Questions? Call 570-271-8092


Next thing you're going to be telling me it's not OK to embed with a 1 foot 
tall bunsen burner open flame and a Coke on the cold tray!

On Fri, Jun 2, 2023 at 7:46 AM Piche, Jessica via Histonet < 
histonet@lists.utsouthwestern.edu> wrote:

> It also doesn't set a good impression for everyone else in the lab
> since they can't have a coffee at their stations. If it could be done,
> I wouldn't want everyone else's coffees on my desk either,  but I'd
> have to because I'd want to make it fair to everyone. If only it was safe for 
> all of us
>
> Have a great weekend everyone!
>
> Jessica
>
> Jessica Piche, HT(ASCP)
> Waterbury Hospital Histology Laboratory Histology Team Leader
> 203-573-7167
> 
> From: McLaughlin, Stacy L. via Histonet
>  >
> Sent: Friday, June 2, 2023 6:09 AM
> To: Hannen, Valerie ;
> Histonet@lists.utsouthwestern.edu 
> Subject: Re: [Histonet] Coffee at the desk
>
> [EXTERNAL MSG]
>
> It is not allowed.  The area you want food/drink has to be completely
> separate- floor to ceiling walls and a door.
>
> Stacy McLaughlin, HT(ASCP) QLScm
> Histology Supervisor
> Cooley Dickinson Hospital
> 30 Locust Street
> Northampton, MA 01060
> Office:  (413)582-2019
> Lab:  (413)582-2179
> smclaughl...@cooleydickinson.org
>
>
>
>
> -Original Message-
> From: Hannen, Valerie via Histonet 
> Sent: Thursday, June 1, 2023 3:40 PM
> To: Histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Coffee at the desk
>
> External Email - Use Caution
>
> Is there any regulation/ reason why a Histology supervisor whose desk
> is in the department and the desk area is taped off and designated as
> a "clean area (  meaning no chemicals or specimens cross the taped off
> area) can't have a closed coffee cup at their desk??  We are having a
> debate in our Lab and I wanted to get a consensus.
>
> Thanks in Advance,
>
> Valerie
>
> Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish
> Medical Center
> 951 N. Washington Avenue
> Titusville, Florida 32796
> P: 321-268-6333  Ext. 7506
> F: 321-268-6149
> valerie.han...@parrishmed.com
>
> http://secur/
> e-web.cisco.com%2F1MKofFBWCX-k1t-x5UFUeTPKR9-7S0h6KjRij8josPZ2RnVifr3g
> Tc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKY
> CLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5X
> UvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZ
> IGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq8
> 6VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com=05%7C01%7Cakb
> itting%40geisinger.edu%7C4f92290a707e4df1d87108db63a05f02%7C37d46c567c
> 664402a16055c2313b910d%7C0%7C0%7C638213312668777202%7CUnknown%7CTWFpbG
> Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%
> 3D%7C3000%7C%7C%7C=%2BQknLpXaFEZ0ilOK2JVWdUzUwrSmCapdNh1SOTmkkn0
> %3D=0
> <
> http://secur/
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> Tc1saxu3dyVWo2u1TBp27RL4TG4m00fPK-aMvSGt1KMvksd0uMNwzg0YgrcASbw4a__wKY
> CLUeJQ4KshW7gatdcNBK5bvqLtpR_YEgRYm_x9TFyIaO4LaRoJnbMB_8cll_HBv6xDv_5X
> UvRtZZIlXJ5hDvnqL91RH582gKyn4mrFltbkGkwjSa8xPkxsqUqo3Y6g_kP8N6wge5a9IZ
> IGelovNZ4x9adKUmrxEK23PZpgTWXsDq7IVchU4WumWhv-jZw3G2wkA_0sbXwArMtcuYq8
> 6VfPp5oSQ8Q%2Fhttp%253A%252F%252Fwww.parrishmed.com=05%7C01%7Cakb
> itting%40geisinger.edu%7C4f92290a707e4df1d87108db63a05f02%7C37d46c567c
> 664402a16055c2313b910d%7C0%7C0%7C638213312668777202%7CUnknown%7CTWFpbG
> Zsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%
> 3D%7C3000%7C%7C%7C=%2BQknLpXaFEZ0ilOK2JVWdUzUwrSmCapdNh1SOTmkkn0
> %3D=0
> >
>
> ___
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> 

Re: [Histonet] [External] Clarification about Immunohistochemistry

[Bitting, Angela K. via Histonet]

We use an Azure B counterstain. Melanin has a greenish tinge after 
counterstaining.

-Original Message-
From: jayalakshmy p.s via Histonet  
Sent: Monday, April 11, 2022 11:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Clarification about Immunohistochemistry

External: Be careful with links and files. If suspicious, alert the Information 
Security Office. Questions? Call 570-271-8092


Hai all Histonetters
 Please anybody clarify my this doubt if possible.
When doing Immunohistochemistry for confirmation of Melanoma with DAB 
chromogen(brown color)the interpretation is not possible because of obscuring 
by the dense melanin pigment. We dont have any other color chromogen. I tried 
ihc after bleaching but the section gets detached even from charged slides. Is 
there any other effective way to do this?
Thanks in advance
Regards
Dr. P S Jayalakshmy
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Re: [Histonet] [EXTERNAL] Re: contents of Histonet digest

[Maimone-Schoen, Michele via Histonet]

Who is your vendor?  Thanks for this information.

From: Cooper, Brian 
Sent: Friday, March 4, 2022 10:42 AM
To: Maimone-Schoen, Michele ; histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] contents of Histonet digest

Our formalin vendor is sending us certificates of analysis with every shipment 
now. We review these forms to ensure the lot is acceptable and file them in a 
binder.

Thanks,

Brian Cooper
Histology Supervisor
Children's Hospital Los Angeles
Sent from my mobile

On Mar 4, 2022 7:18 AM, "Maimone-Schoen, Michele via Histonet" 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:
CAUTION: BE CAREFUL WITH THIS MESSAGE*
This email came from outside CHLA. Do not open attachments, click on links, or 
respond unless you expected this message and recognize the email address: 
histonet-boun...@lists.utsouthwestern.edu.

How are labs addressing the new CAP standard ANP.10041: Quality of Formalin 
Monitoring?   Thank you.


Michele Maimone-Schoen, MS
Manager, Anatomic Pathology
New York-Presbyterian Hospital
525 East 68th Street, Starr 1003
New York, NY  10065
212-746-2633 (Office)
212-746-5007 (Fax)
646-856-1738 (Cell)

Be Amazing!

--
This electronic message is intended to be for the use only of the named 
recipient, and may contain information that is confidential or privileged.  If 
you are not the intended recipient, you are hereby notified that any 
disclosure, copying, distribution or use of the contents of this message is 
strictly prohibited.  If you have received this message in error or are not the 
named recipient, please notify us immediately by contacting the sender at the 
electronic mail address noted above, and delete and destroy all copies of this 
message.  Thank you.
code:d34y
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Re: [Histonet] [EXTERNAL] Histonet Digest, Vol 219, Issue 3

[Michael Dessoye via Histonet]

Based on my experience with this stainer, you may want to think about
replacing.  The water valve assembly/manifold (and associated parts) are
notorious for having expensive parts fail, especially the very expensive
manifold.  I'd look elsewhere for a replacement.

-- Forwarded message --
From: "Cathy M. Mathis/Comparative Medicine" 
To: "histonet@lists.utsouthwestern.edu" 
Cc:
Bcc:
Date: Thu, 3 Feb 2022 21:15:35 +
Subject: [Histonet] 5 Yr old Leica ST5020 is down
Hello Histology friends,
We are a research lab with a 5 year old Leica ST5020 autostainer that looks
like new.  We run less than 5000 slides a year on it.  We noticed that (of
course just after the warranty ended) that one of the water rinse stations
stayed full of water all the time, even when the instrument was off.  It
may have been like this from the beginning and we just did not notice it.
Leica quoted a $6000 repair but said we could run it as is.  So, we skipped
the repair and went on using it.
Now the instrument is completely down and the repair is a new "slave
controller board" for $17,600, stepper motor for arm, etc. with total quote
for over $23,800.  Has anyone else had major issues with this instrument
over time.  We are trying to decide whether to fix it or go for a new
stainer.  I'm kinda wondering if we got a lemon.
Thank you for your time,
Cathy

Cathy M. Mathis
Lab Technician IV
Dept. of Pathology \  Comparative Medicine
Wake Forest School of Medicine
p 336.716.1538  \  f 336.716.1515
cmmat...@wakehealth.edu
www.wakehealth.edu

On Fri, Feb 4, 2022 at 1:00 PM 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> Today's Topics:
>
>1. 5 Yr old Leica ST5020 is down
>   (Cathy M. Mathis/Comparative Medicine)
>2. For those of you interested in travel work! (HistoTrek)
>
>
>
> -- Forwarded message --
> From: "Cathy M. Mathis/Comparative Medicine" 
> To: "histonet@lists.utsouthwestern.edu"  >
> Cc:
> Bcc:
> Date: Thu, 3 Feb 2022 21:15:35 +
> Subject: [Histonet] 5 Yr old Leica ST5020 is down
> Hello Histology friends,
> We are a research lab with a 5 year old Leica ST5020 autostainer that
> looks like new.  We run less than 5000 slides a year on it.  We noticed
> that (of course just after the warranty ended) that one of the water rinse
> stations stayed full of water all the time, even when the instrument was
> off.  It may have been like this from the beginning and we just did not
> notice it.  Leica quoted a $6000 repair but said we could run it as is.
> So, we skipped the repair and went on using it.
> Now the instrument is completely down and the repair is a new "slave
> controller board" for $17,600, stepper motor for arm, etc. with total quote
> for over $23,800.  Has anyone else had major issues with this instrument
> over time.  We are trying to decide whether to fix it or go for a new
> stainer.  I'm kinda wondering if we got a lemon.
> Thank you for your time,
> Cathy
>
> Cathy M. Mathis
> Lab Technician IV
> Dept. of Pathology \  Comparative Medicine
> Wake Forest School of Medicine
> p 336.716.1538  \  f 336.716.1515
> cmmat...@wakehealth.edu
> www.wakehealth.edu
>
>
>
>
>
>
>
> -- Forwarded message --
> From: HistoTrek 
> To: histonet@lists.utsouthwestern.edu
> Cc:
> Bcc:
> Date: Thu, 3 Feb 2022 20:20:33 -0500
> Subject: [Histonet] For those of you interested in travel work!
> Hi histology friends!
>
> With all the staffing shortages, overtime, and pandemic nonsense, it's
> very likely that you’ve heard about travel work a handful of times.
>
> I am a travel histotechnologist who started traveling after COVID19 hit
> and the dynamic in my hospital lab changed A LOT. I was ready for
> something new!
>
> I have been working on a *Getting Started with Travel Work* series on my
> blog and would like to invite everyone interested in travel work to
> check it out!
>
> I publish every Wednesday, and the getting started series is nearly
> complete.
>
> I’d love to get some feedback from the community about what is important
> to you, what you’d like to read about, and how I can help make the
> transition into travel work a little easier.
>
> Please check out the website at https://www.histotrek.com, the travel
> series at https://histotrek.com/start-here/, and let me know your
> feedback! I am on all social media platforms at HistoTrek, or you can
> 

Re: [Histonet] [EXTERNAL] Histonet Digest, Vol 213, Issue 19

[Maimone-Schoen, Michele via Histonet]

Open Positions - New York Presbyterian Hospital/Weill Cornell Division
Histotechnologist - Nights 12 a - 8 a
Histology Supervisor -  2 p - 10 p
Thanks

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Thursday, August 26, 2021 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Histonet Digest, Vol 213, Issue 19

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit

https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!Aut6IJkzM0Y!5RMzGQXqeFtJfNoFy_jB2s-G_nIbNbRqBmhHkvS9QPX3Epf8FqXfg85O1CsvqCa6$
or, via email, send a message with subject or body 'help' to
histonet-requ...@lists.utsouthwestern.edu

You can reach the person managing the list at
histonet-ow...@lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: 
Contents of Histonet digest..."


Today's Topics:

   1. Copy of an old EM resin paper? (Morken, Timothy)
   2. Histopeeps!! RELIA Histology Careers Newsletter - Check   out
  the Spotlight Positions. (Pam Barker)
   3. Cutting protocol for gender dysphoria (Spoon, Victoria)


--

Message: 1
Date: Wed, 25 Aug 2021 17:01:00 +
From: "Morken, Timothy" 
To: Histonet 
Subject: [Histonet] Copy of an old EM resin paper?
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Can anyone supply me a copy of this paper on epoxy resins? I can't find it 
anywhere on line and our library does not have that journal.

Glauert AM: Epoxy Resins: An update on their selection and use.  Microscopy and 
Analysis 15-20 Sept 1991.




Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center



--

Message: 2
Date: Wed, 25 Aug 2021 13:48:34 -0400
From: "Pam Barker" 
To: "Histopeeps Histonet" 
Subject: [Histonet] Histopeeps!! RELIA Histology Careers Newsletter -
Check   out the Spotlight Positions.
Message-ID: <000701d799d9$7aa0f200$6fe2d600$@earthlink.net>
Content-Type: text/plain;   charset="iso-8859-1"

Hello Histopeeps,

I hope you are having a great week! It?s the end of the summer.  Can you 
believe it?

Next week is Labor Day weekend!

Any thoughts on making a move in the near future?

I am already talking to people who are looking at travel contracts ending and 
others who are planning for moves after Christmas.

 

I have great opportunities with some of the cream of the crop of employers 
Nationwide

I have opportunities in:

v Colorado, Illinois, Florida, California, Texas, Virginia, Tennessee and 
Maryland.

My clients are looking for:

v Managers, Supervisors, Quality Specialists, IHC Specialists, Histotechnicians 
and Histotechnologists.

I am Sooo excited about the opportunities I am working on and here?s why:

1.  My clients are excited to meet you and ready to make a decision
right away.
2.  Most of these positions offer up to 10K in bonuses and relocation
assistance!!
3.  If you have plans made for time off you don?t have to cancel them!!
4.  If you are traveling my clients will wait until your contract ENDS!
5.  These are some of the top employers in their areas and I have techs
that have been working in some of these places for YEARS!
6.  The Florida license is easier than EVER to get.  Here?s the link:
https://urldefense.com/v3/__http://www.floridasclinicallabs.gov__;!!Aut6IJkzM0Y!5RMzGQXqeFtJfNoFy_jB2s-G_nIbNbRqBmhHkvS9QPX3Epf8FqXfg85O1BeRAyHe$
  

 

Here Are My Spotlight Opportunities:

v Quality Specialist   Mission Viejo, CA

v IHC Specialist  Chicago Suburbs ? BRAND NEW LAB!!

v AP Supervisor  San Diego, CA

v Histology Supervisor   Sarasota, FL

v Histology Supervisor  Ft. Myers, FL

v Histotechs  Aurora, CO

 

 

Histopeeps!! I also have some amazing job opportunities in:

Florida

?  Panama City   Dermpath/Days

?  Ft. Myers  Dermpath/Days

?  Ft. Myers  Night Shift Supervisor

?  Ft. Myers  IHC specialist/Days

?  Ft. Myers  Histotech/ all shifts Full time part time per diem

?  Ft. Myers  Histotech /All Shifts New Grads welcome

California

?  Aliso ViejoHistotech/ all shifts Full time part time per diem

?  Aliso ViejoHistotech /All Shifts New Grads welcome

?  San Diego  Histotech/ all shifts Full time part time per diem

?  San Diego  Histology Supervisor ? Brand New Lab!!

?  Modesto Histotech ? nights

?  Modesto IHC Specialist

 

And New Opportunities coming in on a daily basis!!

 

All of these clients are offering full time permanent positions with excellent 
compensation packages including VERY competitive pay rates, fantastic benefits, 
relocation and/or sign on bonuses (Up 

Re: [Histonet] [External]: Cell Line Tracking

[Rosa, Taylor via Histonet]

Hello Igor,

I used to work in a lab that tracked several hundred cell lines and I can tell 
you about the tracking system they used if you'd like. It was rudimentary but 
quite effective and the principles behind it could probably be 
updated/streamlined. Please feel free to send me an email at 
taylor.r...@crl.com for more information.

Thanks,

Taylor C. Rosa, MS
Associate Research Scientist
Pathology Services | Charles River 
4025 Stirrup Creek Drive, Suite 150, Durham, NC 27703
P: 919.206.7041 | F: 919.206.7001
taylor.r...@crl.com | www.criver.com
LinkedIn | Twitter | Facebook | Eureka

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: 09-Jun-2021 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External]: Histonet Digest, Vol 211, Issue 9

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit

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or, via email, send a message with subject or body 'help' to
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You can reach the person managing the list at
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When replying, please edit your Subject line so it is more specific than "Re: 
Contents of Histonet digest..."


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Re: [Histonet] [EXTERNAL] Re: Job Opportunities USA Jobs

[Newman-Bodden, Cheryl L. via Histonet]

GS 9 and no, you do not need a FL license for the VA.
You do however, have to have an ASCP (American Society for Clinical Pathology) 
HTL (Histotechnologist) license.

From: Jay Lundgren 
Sent: Friday, April 23, 2021 12:01 PM
To: Newman-Bodden, Cheryl L. 
Cc: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Job Opportunities USA Jobs

Just curious, do you have to have a Florida license to work for the VA in 
Florida?

Do you know the GS rating of the job?
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Re: [Histonet] [EXTERNAL] Histonet Digest, Vol 204, Issue 2

[Maimone-Schoen, Michele via Histonet]

Did you advertise on the ASC site?

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Wednesday, November 4, 2020 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Histonet Digest, Vol 204, Issue 2

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Today's Topics:

   1. Cytotechs needed!  Histopeeps! Can you help? (Pam Barker)


--

Message: 1
Date: Wed, 4 Nov 2020 10:25:53 -0500
From: "Pam Barker" 
To: "Histopeeps Histonet" 
Subject: [Histonet] Cytotechs needed!  Histopeeps! Can you help?
Message-ID: <042101d6b2be$c8d8cc70$5a8a6550$@earthlink.net>
Content-Type: text/plain;   charset="us-ascii"

Hi Histopeeps!
I hope you are having a great week.  I was hoping someone out here on histonet 
can help me.  Recently, several of my clients have asked for assistance in 
recruiting cytotechs.
I would love to help them but don't have a lot of connections in that field.

Here is the help I need:
1.  Do you know any cytotechs that might be interested?
2.  Do you know any cytotechs that might know anyone who might be
interested
3.  Do you know of any groups or forums I should join to reach
cytotechs.
Here is the deal you help me I help YOU!
My normal referral fee is 250.00
For this search I am sweetening the pot:
If you refer a cytotech that I place you will earn 500.00 If you refer a 
cytotech who refers someone that I place you will each earn
250.00
Thanks for taking the time to read my post and Thanks in Advance if you can 
help me!

Thanks-Pam

Right Time, Right Place, Right Move with RELIA!
*15 Years!*
Celebrating 15 years of service exclusively to the Histology Community!

Thank You!
 Pam M. Barker
Pam Barker
President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in 
Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net   
https://urldefense.com/v3/__https://www.facebook.com/RELIASolutionsforhistologyprofessionals__;!!Aut6IJkzM0Y!-qdbkcnFhy4sZAqVtrb8BPW5K2_3ZTDixdB8hZFOXzurxzQgaO0BmAqURVzKDb_A$
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check out our latest opportunities at:
https://urldefense.com/v3/__http://www.jobvertise.com/members/relia1__;!!Aut6IJkzM0Y!-qdbkcnFhy4sZAqVtrb8BPW5K2_3ZTDixdB8hZFOXzurxzQgaO0BmAqURaahSz1y$
#jobs4myhistopeeps
#ilovemyhistopeeps
#histopeeps
Follow my hashtags and make your day great and your career greater!!





--

Subject: Digest Footer

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End of Histonet Digest, Vol 204, Issue 2


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Re: [Histonet] [External] Re: hexamethyleneimine

[Dessasau, Evan D. via Histonet]

Hi LeRoy, we use it in the GMS stain.  It is the Methenamine solution.

Thank you,
E-van

Yerkes National Primate Research Center
Emory University
954 Gatewood Road
Atlanta, GA  30329, USA
 (404) 727-7744 (on campus 7-7744) Lab
edde...@emory.edu



-Original Message-
From: LEROY H BROWN via Histonet 
Sent: Sunday, October 11, 2020 6:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] Re: [Histonet] hexamethyleneimine

What do you use hexamethyleneimine for in your lab.   I found an old bottle
of this reagent and cannot recall why I have it.

It must be used in making up a stain but I am not remembering what stain.

Anyone know?

Thanks

LeRoy Brown HT(ASCP)HTL



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Re: [Histonet] [External] Tissue processor advice

[Tony Auge via Histonet]

I went through 2 ASP300, The first model not the S and they are terrible! I
had a sister lab that got a new/used ASP300S and the hard drive died within
a couple months. I would really recommend against them. The only Leica
certified repair people in the Phoenix area would not work on my ASP300
because they break so often and they didn't want to be responsible for it.

On Wed, Sep 16, 2020 at 11:52 AM Daniel Pesino via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Colleen -
>
> I've used both and I enjoyed both machines. The Leica instrument feels a
> bit more modern and the reagent management system is useful for reagent
> changing schedules. The changing of reagents and wax is pretty easy with
> the drainage hose. I've rarely had any issues with them, but have had
> plenty of issues with the Peloris instruments (made by Leica).
>
> The VIP is a workhorse. Very rarely have we had problems with the
> instrument. It's very reliable, but not quite as "flashy" as the Leica
> instrument.
>
> I'm not sure about parts for the VIP, but if you don't have a service plan
> for the ASP300S, be prepared to pay out the nose for parts. I was quoted
> over $1,000 for a wax drain hose that was literally a piece of plastic
> piping with a simple connector on it.
>
> Best,
>
> Dan
>
> Daniel Pesino, HTL(ASCP)QIHCCM
> Senior Histotechnologist
> Trinity Health Of New England
> daniel.pes...@trinityhealthofne.org
> W  860-714-4675
>
> 114 Woodland Street
> Hartford, CT 06105
> TrinityHealthOfNE.org | Facebook | Twitter | Instagram
>
>
>
>
>
> -Original Message-
> From: Colleen Forster via Histonet 
> Sent: Wednesday, September 16, 2020 1:17 PM
> To: histonet-request 
> Subject: [External] [Histonet] Tissue processor advice
>
> Warning:  This email originated from the Internet!
> DO NOT CLICK links if the sender is unknown, and NEVER provide your
> password.
>
> HEllo Histoneters,
>
> I am looking at replacing my VIP2000, porr girl finally quit on me.
>
> The two processors I am looking at:
>
> Leica ASP300S
> Sakura VIP5
>
> Any of you out there who have used either of these or both, can you give
> me pros , cons, yes, noI am just looking for experiences those who have
> used them can share.
>
> Thank you in advance.
>
> Colleen Forster HT(ASCP)QIHC
> BLS Histology and IHC Laboratory
> Jackson Hall, Room 2-155
> 612-626-1930
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>
>
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-- 

Tony Auge HTL (ASCP)CM QIHC
Cell: (651) 373-4768
Email: tony.a...@gmail.com
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Re: [Histonet] [External] Tissue processor advice

[Daniel Pesino via Histonet]

Hi Colleen -

I've used both and I enjoyed both machines. The Leica instrument feels a bit 
more modern and the reagent management system is useful for reagent changing 
schedules. The changing of reagents and wax is pretty easy with the drainage 
hose. I've rarely had any issues with them, but have had plenty of issues with 
the Peloris instruments (made by Leica).

The VIP is a workhorse. Very rarely have we had problems with the instrument. 
It's very reliable, but not quite as "flashy" as the Leica instrument.

I'm not sure about parts for the VIP, but if you don't have a service plan for 
the ASP300S, be prepared to pay out the nose for parts. I was quoted over 
$1,000 for a wax drain hose that was literally a piece of plastic piping with a 
simple connector on it.

Best,

Dan

Daniel Pesino, HTL(ASCP)QIHCCM
Senior Histotechnologist
Trinity Health Of New England
daniel.pes...@trinityhealthofne.org
W  860-714-4675

114 Woodland Street
Hartford, CT 06105
TrinityHealthOfNE.org | Facebook | Twitter | Instagram





-Original Message-
From: Colleen Forster via Histonet  
Sent: Wednesday, September 16, 2020 1:17 PM
To: histonet-request 
Subject: [External] [Histonet] Tissue processor advice

Warning:  This email originated from the Internet!
DO NOT CLICK links if the sender is unknown, and NEVER provide your password.

HEllo Histoneters,

I am looking at replacing my VIP2000, porr girl finally quit on me.

The two processors I am looking at:

Leica ASP300S
Sakura VIP5

Any of you out there who have used either of these or both, can you give me 
pros , cons, yes, noI am just looking for experiences those who have used 
them can share.

Thank you in advance.

Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
612-626-1930
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Re: [Histonet] [External] QIHC Prep

[Daniel Pesino via Histonet]

Hi Ariel -

Congratulations on this big step in your career! I used the QIHC Prep Course 
program by Joe Myers. The course is excellent and comes with a very helpful 
resource guide. You can find it on the NSH website.

Best of luck!

Daniel Pesino, HTL(ASCP)CM QIHC CM
Senior Histotechnologist
Trinity Health Of New England
daniel.pes...@trinityhealthofne.org
W  860-714-4675

114 Woodland Street
Hartford, CT 06105
TrinityHealthOfNE.org | Facebook | Twitter | Instagram

-Original Message-
From: Ariel Liberda via Histonet  
Sent: Monday, September 14, 2020 10:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] QIHC Prep

Warning:  This email originated from the Internet!
DO NOT CLICK links if the sender is unknown, and NEVER provide your password.

Hello all!
I hope everyone is staying safe!

I'm looking to get my QIHC and I was wondering what prep materials everyone 
found most useful? I've looked at the histonet archives and everything I found 
was mostly out of date. I'm also looking for info about the test itself. How 
difficult did you find it? Someone mentioned that they believed it was a test 
taken with notes available but I haven't been able to find confirmation of 
this. Any info will be helpful! I've worked with IHC's for a few years now so 
I'm looking forward to getting the official accreditation!

Thank you all in advance! Histonet is such a wonderful wealth of knowledge!
-Ari

--

Ariel Liberda, HT(ASCP)
Lead Histotech

CTALab
c. 
[503.906.7300](https://urldefense.proofpoint.com/v2/url?u=https-3A__webmail.networksolutionsemail.com_edgedesk_cgi-2Dbin_tel-3A503.906.7300=DQMFAg=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM=HKyZElUZ9A6Yyf6DYydB4h3JuSpSmPINBXYcEI2kzIM=kk9btPrCg75YkgEZBePfoDnCBgWXID9HnQfCIK51c80=Sd5gQGqV6e2f-nZgaLPMCVPVBCSZEDZQCN-XstC2-74=)
 | f. 
[503.245.8219](https://urldefense.proofpoint.com/v2/url?u=https-3A__webmail.networksolutionsemail.com_edgedesk_cgi-2Dbin_tel-3A503.245.8219=DQMFAg=euGZstcaTDllvimEN8b7jXrwqOf-v5A_CdpgnVfiiMM=HKyZElUZ9A6Yyf6DYydB4h3JuSpSmPINBXYcEI2kzIM=kk9btPrCg75YkgEZBePfoDnCBgWXID9HnQfCIK51c80=Ba4mH8xxABXuh3rvnefIcx6NSVFWm_tOxUI1yrS533k=)
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Re: [Histonet] [External] Leica Bond, ASP6025 Processor parts

[Daniel Pesino via Histonet]

You should see how much they want for a wax drain hose for the Peloris 
processors... A tube and a nozzle for +$1,000. I ended up building my own with 
parts from Home Depot for about $30.

We use Perkins Biomedical for our preventative maintenance and parts. They do 
repairs throughout the northeast, but should be able to send you parts. They 
may have a spare retort lid that they can quote you. You will probably need 
someone else to install it in California.

You can check them out here:

https://perkinsbiomed.com/

Or you can reach them at 800-975-7456. They are super professional and 
knowledgeable.

Daniel Pesino, HTL(ASCP)CMQIHCCM
Senior Histotechnologist
Trinity Health Of New England
daniel.pes...@trinityhealthofne.org
W  860-714-4675

114 Woodland Street
Hartford, CT 06105
TrinityHealthOfNE.org | Facebook | Twitter | Instagram

-Original Message-
From: Paula via Histonet  
Sent: Friday, August 21, 2020 2:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Leica Bond, ASP6025 Processor parts

Warning:  This email originated from the Internet!
DO NOT CLICK links if the sender is unknown, and NEVER provide your password.

Hi all,



Does anyone know of a Biomed company that makes and/or sells parts for the Bond 
III and the ASP6025 processor?



It's amazing how much Leica wants for their parts. For example, the waste 
bottle used to collect the buffer waste is $674 and the seal for the retort's 
lid is $597.



Hope someone can help..thanks in advance.



Paula

Bio-Path Medical Group

Fountain Valley, CA

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Re: [Histonet] [EXTERNAL] Brain and spinal cord

[Stedman, Nancy via Histonet]

Hi – I use Excell Plus (alcohol based) for small brains (dog size and smaller). 
 I book them after 24 hours and then let them sit another 24 hours or more 
depending on the size, and changing the Excell.  It’s definitely not good as 
formalin, but my cases are veterinary necropsies and usually already have some 
autolysis already when they are submitted.  IHC seems to work just fine.  Have 
not tried ISH or nucleic acid extraction but the company that makes Excell Plus 
says it is suitable for these applications.  I have tried other alcohol based 
fixatives for small brains and none have been as good as Excell Plus.

-Nancy Stedman


-Original Message-
From: Yahoo via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, May 15, 2020 4:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] Brain and spinal cord

Hi All! 
I’m looking for some suggestions please on fixation for brain tissue and spinal 
cord submissions from necropsies. We are currently using 10% NBF and ask our 
pathologists to leave the samples overnight (but that doesn’t always happen!!). 
Does anyone use alcohol-based fixatives? And if so, how long? Does it affect 
IHC or any other staining? Do you still process with other routine biopsies (14 
hour program)?
Thanks!

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Re: [Histonet] [External] IHC DAB

[Daniel Pesino via Histonet]

Hi Kristy,

What platform and detection kit are you using? Would you mind sharing your DAB 
protocol?

Thank you,


Daniel Pesino, HTL(ASCP)CM QIHC CM
Senior Histotechnologist
Trinity Health Of New England
daniel.pes...@trinityhealthofne.org
W  860-714-4675

114 Woodland Street
Hartford, CT 06105
TrinityHealthOfNE.org | Facebook | Twitter | Instagram

-Original Message-
From: Kristy Castillo via Histonet  
Sent: Friday, May 1, 2020 11:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] IHC DAB

Warning:  This email originated from the Internet!
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Hi Histonetters,

We are starting our IHC (fun times), we are having trouble with the DAB 
lighting up.  Processed for 5 thru 10 minutes and still nothing.  Our permanent 
red is working just fine.  Any ideas.

Thank you!

Kristy
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error in a separate email.


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Re: [Histonet] [EXTERNAL Sender] Pap slides

[Hannen, Valerie via Histonet]

Yes, they can be left overnight in 95% alcohol before staining.  We do at times 
and have not had any report of altered quality.

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 13, 2020 12:52 PM
To: Histo List
Subject: [EXTERNAL Sender] [Histonet] Pap slides


This message came from an external source. Please do not click links or open 
attachments if unexpected or unusual.

Begin Original Message:

--
Can pap slides be left in 95% alcohol overnight to be stained the next
morning without affecting the quality of the slides? Or should they be
moved to xylene then run back prior to staining?

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] [EXTERNAL] Pap slides

[Perl, Alison via Histonet]

We routinely leave our ThinPrep Paps in 95 overnight and stain the next day. If 
I remember our Hologic training correctly, they said the slides can be left up 
to 5 days - best to check with an FAS before trying it, though

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager 
CareMount Medical
(914) 302-8424
ap...@caremount.com


-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 13, 2020 12:52 PM
To: Histo List
Subject: [EXTERNAL] [Histonet] Pap slides

Can pap slides be left in 95% alcohol overnight to be stained the next
morning without affecting the quality of the slides? Or should they be
moved to xylene then run back prior to staining?

-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] [EXTERNAL] Re: Incomplete cross sections of all tissue in blocks

[Perl, Alison via Histonet]

Each of our techs is responsible for fully facing their blocks and getting a 
representative section. If the docs see something is amiss (epidermis missing, 
incomplete cross section, etc), they can order a Technical Recut rather than a 
routine Recut. Unfortunately, it sounds like you (or someone else of 
authority), has to give some feedback/coaching that the sections are 
insufficient, particularly if it's a recurring problem. Is it one tech that 
needs a 1-on-1 convo, or a meeting with the whole staff to address widespread 
issues?

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager 
CareMount Medical
(914) 302-8424
ap...@caremount.com


-Original Message-
From: John Garratt via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, March 3, 2020 12:02 PM
To: Amy Self; histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Incomplete cross sections of all tissue in 
blocks

I suggest that each histotech is responsible for the blocks they cut and they 
cut the deepers on the their own blocks when they are requested. With feedback 
on the reason for the deeper from the pathologists they (the techs) will become 
more confident and learn how deep to cut.

John

On Tue, Mar 3, 2020 at 7:31 AM, Amy Self via Histonet 
 wrote:

> Good Morning HistoNetters,
>
> I am reaching out to the histonet in hopes to get some suggestions from you 
> on how to handle incomplete cross-sections of tissue in blocks. We are a 
> small lab so this has not been an issue in the past but now that we are 
> growing and our staff has increased I am getting feed-back from pathologist 
> that the sections of tissue are not complete. They are asking for too many 
> deepers that possibly could be avoided if it was cut deep enough to begin 
> with. I have been given some managerial type duties – which I don’t like 
> cause I know nothing about managing people and I need to approach this but I 
> need to approach this issue correctly. Do you have the histotech compare 
> his/her cut slides to the block to make sure that a complete cross-section is 
> obtained and is this documented somehow? Any and all suggestions I need.
>
> Thanks in advance for your help and as always you all rock.. 
>
> Amy Self
> Histology Lab Senior Tech
> Lab
> Tidelands Georgetown Memorial Hospital
> 606 Black River Road
> Georgetown, SC 29440
> (843) 520-8711
> as...@tidelandshealth.org
> Our mission: We help people live better lives through better health.
>
> NOTE:
> The information contained in this message may be privileged, confidential and 
> protected from disclosure. If the reader of this message is not the intended 
> recipient, or an employee or agent responsible for delivering this message to 
> the intended recipient, you are hereby notified that any dissemination, 
> distribution or copying of this communication is strictly prohibited. If you 
> have received this communication in error, please notify us immediately by 
> replying to this message and deleting it from your computer.
> Thank you.
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Re: [Histonet] External alarm for Tissue-Tek VIP E300

[John Garratt via Histonet]

There are wiring ports on the E3000 for an external alarm. You have to decide 
where the alarm can be monitored 24/7 at your site. If you mean a wireless 
monitoring system, and these are systems, take a look at Thermo. 
https://www.thermofisher.com/ca/en/home/life-science/lab-equipment/wireless-monitoring-systems.html

John


www.cpqa.ca

‐‐‐ Original Message ‐‐‐
On Thursday, February 27, 2020 12:21 PM, Katie via Histonet 
 wrote:

> I am looking to get an external alarm for our tissue processor. Any
> suggestions? Thanks so much!
>
> Katie Riley-Hamilton
> Technical Supervisor of Dermatopathology
>
> Puyallup Dermatology Clinic
>
> ka...@puyallupderm.com
>
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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[Histonet] External alarm for Tissue-Tek VIP E300

[Katie via Histonet]

I am looking to get an external alarm for our tissue processor. Any
suggestions? Thanks so much!

Katie Riley-Hamilton
Technical Supervisor of Dermatopathology

Puyallup Dermatology Clinic

ka...@puyallupderm.com
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Re: [Histonet] [EXTERNAL Sender] histologists assisting with transplants

[Rena Fail via Histonet]

when I worked, I did a lot of histology on both native and donor livers.  I
would not have used the term assisting with a liver transplant but on rare
occasions, I had requests for a rapid Oil red O for fat on the donor  liver
In order for the pathologist to better determine the percentage of fat in
the donor liver. Too much fat and it might not be considered for a
transplant.
Maybe it is just a matter of terminology. After all the pathologist and
surgeon called her in at 3am and may have thanked her for her assistance.
In 30 years I may have done 3 rapid ORO's on donor livers

Rena Fail
retired


On Thu, Feb 13, 2020 at 11:44 AM Hannen, Valerie via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

>
>
> -Original Message-
> From: Hannen, Valerie
> Sent: Thursday, February 13, 2020 10:50 AM
> To: 'Eileen Akemi Allison'
> Subject: RE: [EXTERNAL Sender] [Histonet] histologists assisting with
> transplants
>
> The only way that I have been involved with anything to do with any kind
> of transplant was to do a FS on the donor tissue, to ensure that it was a
> good transplantable organ.
>
>
>
> Valerie Hannen,MLT(ASCP),HTL,SU (FL)
> Section Chief, Histology
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville,Florida 32796
> T: (321)268-6333 ext. 7506
> F: (321) 268-6149
> valerie.han...@parrishmed.com
> www.parrishmed.com
>
>
>
> -Original Message-
> From: Eileen Akemi Allison via Histonet [mailto:
> histonet@lists.utsouthwestern.edu]
> Sent: Thursday, February 13, 2020 7:42 AM
> To: Histonet
> Subject: [EXTERNAL Sender] [Histonet] histologists assisting with
> transplants
>
>
> This message came from an external source. Please do not click links or
> open attachments if unexpected or unusual.
>
> Begin Original Message:
>
> --
> Good morning histoland!
> Just curious, this is definitely a 1st for me.  Received a call this
> morning from my histotech who is on call this week that she was called in
> at 3:30 AM to assist one our pathologists with a liver transplant.  In all
> my years working in this field, histologists have never had anything to do
> with transplants.  Have any of you out there had anything to do with
> transplants?
>
> Akemi Allison, BS, HTL
> Histology Supervisor
> UMC El Paso, TX
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Re: [Histonet] [EXTERNAL Sender] histologists assisting with transplants

[Hannen, Valerie via Histonet]

-Original Message-
From: Hannen, Valerie 
Sent: Thursday, February 13, 2020 10:50 AM
To: 'Eileen Akemi Allison'
Subject: RE: [EXTERNAL Sender] [Histonet] histologists assisting with 
transplants

The only way that I have been involved with anything to do with any kind of 
transplant was to do a FS on the donor tissue, to ensure that it was a  good 
transplantable organ.



Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-Original Message-
From: Eileen Akemi Allison via Histonet 
[mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, February 13, 2020 7:42 AM
To: Histonet
Subject: [EXTERNAL Sender] [Histonet] histologists assisting with transplants


This message came from an external source. Please do not click links or open 
attachments if unexpected or unusual.

Begin Original Message:

--
Good morning histoland!
Just curious, this is definitely a 1st for me.  Received a call this morning 
from my histotech who is on call this week that she was called in at 3:30 AM to 
assist one our pathologists with a liver transplant.  In all my years working 
in this field, histologists have never had anything to do with transplants.  
Have any of you out there had anything to do with transplants?

Akemi Allison, BS, HTL
Histology Supervisor
UMC El Paso, TX
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Re: [Histonet] [EXTERNAL] grossing manuals

[Porter, Douglas via Histonet]

Here are three "grossing manuals" I've found helpful:


http://pathology.ucla.edu/gross-manual


https://www.pathology.med.umich.edu/cutting


https://voices.uchicago.edu/grosspathology/


Douglas A. Porter, HT (ASCP)
Pathologist Assistant
Anatomic Pathology IT Coordinator

Sparrow Center for Laboratory Medicine
Department of Pathology
3392 Patient Care Drive
Lansing, MI 48911

517-371-9481 (phone)
517-371-9540 (fax)

douglas.por...@sparrow.org




-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, January 22, 2020 11:19 AM
To: Histonet 
Subject: [EXTERNAL] [Histonet] grossing manuals

 Warning: This email originated from outside of Sparrow. Do not click links or 
open attachments unless you recognize the sender  and are expecting the message.

--
Posted for a friend...







To my knowledge the best institutional manual is the University of Michigan's 
Cutting Manual.



-Izak Dimenstein 





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Re: [Histonet] [External] Superfrost adhesion problems?

[Cynthia Robinson via Histonet]

We have seen surface tension issues with staining on slides. We had several 
lots and were able to remove the bad lots from our stock and that has helped.


Cindi Robinson HT(ASCP)

Histology Lead

MercyOne Siouxland Medical Center

Dunes Medical Laboratories

101 Tower Road, Suite 220

Dakota Dunes SD 57049


From: Fulton Regan via Histonet 
Sent: Tuesday, December 3, 2019 4:39 PM
To: histonet@lists.utsouthwestern.edu 
Subject: [External] [Histonet] Superfrost adhesion problems?

Warning:  This email originated from the Internet!
DO NOT CLICK links if the sender is unknown, and NEVER provide your password.

Histonetters,

We have heard some anecdotal reports of a recent decrease in the adhesive 
performance of Superfrost slides, with tissue falling off during IHC staining 
on automated instruments.  We use a lot of these slides and we may have 
experienced this problem.  Has anyone else encountered this problem recently?

Thanks.


Regan Fulton, M.D., Ph.D.

Array Science, LLC
475 Gate 5 Road, #102
Sausalito, CA 94965
www.arrayscience.com




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Re: [Histonet] [EXTERNAL Sender] Re: Extremity carrier

[Hannen, Valerie via Histonet]

We have a "Pathport" container ( kind of large) that we use, we place it on a 
(designated) cart. When we are done with the extremity, we place it in a large 
(designated)  biohazard (sharps) container which we store in our walk-in 
refrigerator until time for disposal. 
We return the "Pathport" container and cart  to our Sterile processing 
department, they decontaminate the "Pathport" and it and the cart are returned 
to the O.R. area.  


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-Original Message-
From: KD Bow via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, October 21, 2019 5:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL Sender] Re: [Histonet] Extremity carrier


This message came from an external source. Please do not click links or open 
attachments if unexpected or unusual.

Begin Original Message:

--
I work in a research lab so this may not go over well.  How about using 
a cart and a child size body bag? You would not be able to see through 
the bag and there shouldn't be any smell and you most likely have a cart 
around the place.

-- 

*/-- You are what you do - not what you say --/*

*Karen Bowden
Staff Research Associate II
University of CA, San Diego*

*Department of Medicine*

*9500 Gilman Dr. 0682*

*La Jolla, CA 92093-0682*

*858-534-0575 voice
858-534-2005 fax
**/

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On 10/21/19 1:19 PM, Olszewski, Dawn via Histonet wrote:
> Good afternoon,
>
> I have been tasked to find a better solution to transport extremities from 
> the OR to histology.  We have tried cardboard boxes (no longer allowed) and 
> plastic totes on rollers (too big to store and absorb odors from the legs).  
> The histology lab is located quite a ways from the OR and passes through some 
> main areas of the hospital.  I was wondering what everybody else uses for 
> this issue.  All suggestions are appreciated.  Thank you in advance for any 
> ideas.
>
> Sincerely,
>
> Dawn Olszewski,  HTL(ASCP)QIHC
> Pathology Manager
> South Georgia Medical Center
> 229-259-4830
> dawn.olszew...@sgmc.org
>
>
>
> Dawn Olszewski
> Pathology Manager - Histotechnologist
> Laboratory
>
> [cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png]
>
> South Georgia Medical Center
> 2501 N. Patterson St.
> Valdosta, GA 31602
> 229-259-4830 (O) | 229-560-6191 (M)
> dawn.olszew...@sgmc.org | 
> sgmc.org  >
>
> [cid:facebook_fb_32x32_0a6d9065-09e0-48f7-88c4-b2d459a5e547.png]  >  [cid:twitter_32x32_5a3a5eeb-77c7-4a3c-aa09-b69b41f32bd2.png] 
>   >   [cid:linkedin_ln_32x32_bf1e5dc9-b886-480a-8c8b-ef8a1ae87168.png] 
>   >   [cid:youtube_play_32x32_572cd3ed-390b-48e0-8845-ba1f81953de3.png] 
>   >
>
>
>
>
>
>
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Re: [Histonet] [External] Jeepers Creepers, Coverslipping Peepers!

[Dessasau III, Evan via Histonet]

Hi Sandra, Have you tried Brain Research Laboratories?  We use the 35x50mm 
no.1, 3oz. pack.  We buy them through Mark at PathSupply.  The phone number on 
the box is 617 965 5544.  PathSupply's number is 800 631 3556.
Thank you,

E-van

-Original Message-
From: Sandra Cheasty via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, July 23, 2019 3:49 PM
To: Histonet (histonet@lists.utsouthwestern.edu) 

Subject: [External] [Histonet] Jeepers Creepers, Coverslipping Peepers!

Hello all,
Does anyone have a source for oversized coverglass? (35x50, 
35x60, 45x50, 45x60) We section a lot of globes from the ophthalmology lab, 
some of which are from horses, cows, etc. that are put on the larger slides. 
Fisher used to carry their own brand, but it's apparently discontinued them, 
and their "alternate" product is $50/oz!
Thanks!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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Re: [Histonet] [EXTERNAL Sender] Re: Histonet Digest, Vol 188, Glass plate for AO knife sharpener

[Hannen, Valerie via Histonet]

Unfortunately, I tried to find some as well and they no longer are available.  
Also, a heads up, I no longer can find fine abrasive either, all of the vendors 
that I contacted said they no longer make it. So, as soon as the  fine abrasive 
I have is gone I will be switching over to disposable blades.



Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com


-Original Message-
From: Steve McClain via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, July 11, 2019 4:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL Sender] Re: [Histonet] Histonet Digest, Vol 188, Glass plate 
for AO knife sharpener


This message came from an external source. Please do not click links or open 
attachments if unexpected or unusual.

Begin Original Message:

--
Does anyone know where to find the glass plate for an ancient AO sharpener for 
microtome?



Steve

Steve A. McClain, MD

631-361-4000  Cell 631-926-3655

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Re: [Histonet] [External] Re: SDHB IVD antibody

[Cartun, Richard via Histonet]

We use the antibody at a dilution of 1:50 following low-pH HIER (20 minutes) on 
the Leica Bond Max IHC platform.  Our primary antibody incubation is 15 
minutes.  Detection is Bond Refine HRP with DAB. 

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org

-Original Message-
From: Bitting, Angela K. [mailto:akbitt...@geisinger.edu] 
Sent: Tuesday, June 25, 2019 1:03 PM
To: Cartun, Richard
Subject: RE: [External] Re: [Histonet] SDHB IVD antibody

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Would you share your procedure?


-Original Message-
From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 25, 2019 12:31 PM
To: Emmanuel Geoffrey S Gonzaga
Cc: histonet@lists.utsouthwestern.edu
Subject: [External] Re: [Histonet] SDHB IVD antibody

Cell Marque offers an excellent SDHB rabbit monoclonal (clone EP288) that we 
use in our laboratory.  However, it is not an "IVD".

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 (Office)
(860) 545-2204 (Fax)
richard.car...@hhchealth.org


-Original Message-
From: Emmanuel Geoffrey S Gonzaga via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 25, 2019 11:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] SDHB IVD antibody

EXTERNAL email from Outside HHC! Do NOT open attachments or click links from 
unknown senders.

Hello Histonet!



Does anyone know of a vendor that offers SDHB IVD antibody? It looks like Zeta 
corp. use to offer it, but has since discontinued production.



Thanks



Emmanuel Gonzaga

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Re: [Histonet] [EXTERNAL] Retic staining

[Perl , Alison via Histonet]

Hi Charles
I've had very good luck in the past with Poly Scientific's Gomori's retic kit, 
which is ready-to-use (no ammoniacal preparation!) and very reliable. I haven't 
used AMT's. I wonder if anything changed in the transition to StatLab 
ownership? Making the ammoniacal generally seems to be the trickiest part...

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager 
(914) 302-8424
ap...@cmmedical.com

-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, April 22, 2019 2:57 PM
To: Histo List
Subject: [EXTERNAL] [Histonet] Retic staining

I am having trouble getting my retic stain to come out strong. It is
working but Pathologists are saying they shouldn't have to go to high power
just to see it (and I agree).

I am using a brand new American MasterTech Chandler's Precision kit. I have
followed all the steps correctly (one difficulty is getting the Ammonium
hydroxide to go drop by drop so this may be the issue).

I have tried staining longer in ammoniacal silver. more time in 20%
formalin,  more and less time in gold chloride and sodium thiosulfate.

If anyone has any suggestions or uses this kit with time modifications
please let me know. Willing to try anything to get it working.


-- 

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] [External Sender] Lab Week

[Hannen, Valerie via Histonet]

In addition to the fun ideas shared by others.. we also do cooking contests 
with prizes!!  We do "cake walks" for our staff with the Section Chiefs/ 
supervisors providing the goody for the cake walk... the staff love it, they 
say it helps cheer up their day... thinking maybe they will be the one to win 
the sweet treat!!  We (supervisors) also make up little gift bags for our staff 
to show our appreciation for all of their hard work!!



Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com


-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Thursday, April 18, 2019 8:41 AM
To: Histo List
Subject: [External Sender] [Histonet] Lab Week

WARNING: This message came from an external source. Please do not click links 
or open attachments if unexpected or unusual.

Anyone have any ideas on things I can do in our lab to celebrate lab week?

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] [EXTERNAL] Histonet Digest, Vol 184, Issue 7

[Michele Maimone-Schoen via Histonet]

There are openings at New York Presbyterian Hospital/Weill Cornell Division, 
68th Street, NYC:
  Histotech (Monday-Friday 12pm-8pm)-Must have New York Clinical Laboratory 
License in histology or clinical laboratory technology
  Histotech (Tuesday-Saturday, day flex)-Must have New York Clinical Laboratory 
License in histology or clinical laboratory technology
Please contact me at 212-746-2633 for more details.  Thanks

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Friday, March 8, 2019 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Histonet Digest, Vol 184, Issue 7

Send Histonet mailing list submissions to
histonet@lists.utsouthwestern.edu

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When replying, please edit your Subject line so it is more specific than "Re: 
Contents of Histonet digest..."


Today's Topics:

   1. Really Small acid cabinet? (Morken, Timothy)
   2. Re: Really Small acid cabinet? (William Ahlfeld)
   3. NY-Anatomic Pathology Job Openings (Melissa Owens)
   4. Typo-NY Anatomic Pathology Jobs (Melissa Owens)


--

Message: 1
Date: Fri, 8 Mar 2019 00:11:36 +
From: "Morken, Timothy" 
To: Histonet 
Subject: [Histonet] Really Small acid cabinet?
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Does anyone know of an acid cabinet for just a few 500ml bottles? All I can 
find fit 6 1-liter bottles, no smaller.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of 
Pathology UC San Francisco Medical Center



--

Message: 2
Date: Thu, 7 Mar 2019 19:28:34 -0500
From: William Ahlfeld 
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Really Small acid cabinet?
Message-ID: 
Content-Type: text/plain; charset=us-ascii

Check out Uline part #H-4174

Sent from my iPad



--

Message: 3
Date: Fri, 8 Mar 2019 16:59:45 +
From: Melissa Owens 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] NY-Anatomic Pathology Job Openings
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Happy Friday,

Just spreading the word on some new openings I have in New York. Please contact 
me directly for more details/job description.

Long Island Area (New Hyde Park)


  1.  Grossing Histotech/Pathologist Assistant (Tuesday-Saturday 
10am-6:30pm)-New York License Eligibility Bronx Area


  1.  Pathologist Assistant/Grossing Histotech (Monday-Friday 3pm-11:30pm)- 
Must have New York Clinical Laboratory License or PA license


  1.  Histotech (Monday-Friday 2pm-10:30pm)-Must have New York Clinical 
Laboratory License in histology or clinical laboratory technology


Melissa Owens, CHP (ASA)
President, Laboratory Staffing
Allied Search Partners
T: 888.388.7571 ext. 102
Direct Line:  407.413.9117





--

Message: 4
Date: Fri, 8 Mar 2019 17:10:54 +
From: Melissa Owens 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Typo-NY Anatomic Pathology Jobs
Message-ID:



Content-Type: text/plain; charset="us-ascii"

Sorry there was a typo on location of the last email. Please see correct 
version of my last email.


Happy Friday,



Just spreading the word on some new openings I have in New York. Please contact 
me directly for more details/job description.



Long Island Area (New Hyde Park)



Grossing Histotech/Pathologist Assistant (Tuesday-Saturday 10am-6:30pm)-New 
York License Eligibility



Bronx Area



Pathologist Assistant/Grossing Histotech (Monday-Friday 3pm-11:30pm)- Must have 
New York Clinical Laboratory License or PA license



Histotech (Monday-Friday 2pm-10:30pm)-Must have New York Clinical Laboratory 
License in histology or clinical laboratory technology

Melissa Owens, CHP (ASA)
President, Laboratory Staffing
Allied Search Partners
T: 888.388.7571 ext. 102
Direct Line:  407.413.9117
F: 888.388.7572




--

Subject: Digest Footer

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Re: [Histonet] [External] Re: Another Dispenser Failure

[Bitting, Angela K. via Histonet]

Is there any other way! :-O

Sent from my iPhone

> On Feb 28, 2019, at 3:30 PM, Mark Tarango via Histonet 
>  wrote:
> 
> I hope everyone is using on-slide controls :-)
> 
> On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> 
>> Another one!!!
>> Our Her2 antibody dispenser failed, LOT #E22628
>> This one "supposedly" FDA approved.
>> Roche, why do you continue to lie to consumers of your product?  You claim
>> you've "fixed" the problem but your Ventana dispensers DON'T WORK!
>> This is patient care!  Why don't you care about the customers and patients
>> you are supposed to be serving
>> Shame on you.
>> 
>> Terri L. Braud, HT(ASCP)
>> Anatomic Pathology Supervisor
>> Laboratory
>> Holy Redeemer Hospital
>> 1648 Huntingdon Pike
>> Meadowbrook, PA 19046
>> ph: 215-938-3689
>> fax: 215-938-3874
>> Care, Comfort, and Heal
>> 
>> -Original Message-
>> From: histonet-requ...@lists.utsouthwestern.edu [mailto:
>> histonet-requ...@lists.utsouthwestern.edu]
>> Sent: Thursday, February 28, 2019 1:00 PM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: Histonet Digest, Vol 183, Issue 23
>> 
>> 
>> Today's Topics:
>>   1. H Staining question (Charles Riley)
>>   2. Re: H Staining question (Jay Lundgren)
>>   3. FYI- Roche Ventana users (Cassie P. Davis)
>> Message: 3
>> Date: Thu, 28 Feb 2019 15:23:16 +
>> From: "Cassie P. Davis" 
>> To: histonet 
>> Subject: [Histonet] FYI- Roche Ventana users
>> Message-ID: 
>> Content-Type: text/plain; charset="iso-8859-1"
>> 
>> Hi Histoland,
>>I am biting my tongue HARD and just letting you know so it doesn't
>> happend to you. I just got off the phone with Roche here is the heads-up.
>> If one of their anitbody dispensers fails DO NOT put the antibody in one
>> of their prep kits, as soon as you do they consider it off label use.
>> Call customer service immediately and have them overnight a replacement!
>> Cassandra Davis
>> Histology Technician
>> AP Laboratory
>> 302-575-8095
>> Email:  cda...@che-east.org
>> 
>> 
>> 
>> ___
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>> 
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Re: [Histonet] [External] Re: New York Qualifications for Histology Supervisor

[P Sicurello via Histonet]

I need to clarify my comment about California.  What Histotechs (certified
or not, but not licensed by CA) cannot do is supervise or manage those who
have licenses through the state (Cyotechs, CLS', Phlebotomists, etc.).

Unlicensed personnel are not allowed to supervise/manage licensed personnel.

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

La Jolla, CA 92037
(P): 858-249-5610



*Confidentiality Notice*: The information transmitted in this e-mail is
intended only for the person or entity to which it is addressed and may
contain confidential and/or privileged material.  Any review,
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intended recipient is prohibited.  If you received this e-mail in error,
please contact the sender and delete the material from any computer.


On Fri, Feb 22, 2019 at 5:30 AM Fortin, Joyce 
wrote:

> omg!!
>
> Joyce Fortin
> Histology Supervisor
> Palmdale Regional Medical Center
> 38600 Medical Center Drive
> Palmdale, California  93551
> Phone 661-382-5723
> Fax   661-382-5747
> email:  joyce.for...@uhsinc.com
>
>
> 
> From: Patpxs via Histonet 
> Sent: Thursday, February 21, 2019 9:18 AM
> To: Colleen Forster
> Cc: histonet@lists.utsouthwestern.edu
> Subject: [External] Re: [Histonet] New York Qualifications for Histology
> Supervisor
>
> WARNING: This email is from an external source.
> DO NOT CLICK links or attachments unless you recognize the sender and know
> the content is safe.
> REPORT suspicious emails to reports...@uhsinc.com ASAP.
>
> This is an issue that continues to pop up.   It all starts with the CLIA
> regulations that do not recognize histology professionals as equal to other
> laboratory professionals.  Until the feds change CLIA we will  keep having
> this problem.
>
> I do believe that NSH is petitioning on our behalf to change the CLIA
> regulations.
>
> In California the state does not recognize Histotechs, certified or not,
> so we can’t get licensed and we can’t become lab managers as a result.  All
> thanks to CLIA.
>
> That’s my rant for the day.
>
> Paula
>
> Sent from my iPhone
>
> > On Feb 21, 2019, at 8:58 AM, Colleen Forster via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
> >
> > Wow...this is just crazy~ The regulations leave histology in a real tough
> > spot...
> >
> > Feeling for all of you and yes, for the future histology techs coming up~
> >
> > Colleen Forster
> > U of MN
> >
> > On Thu, Feb 21, 2019 at 10:52 AM Perl , Alison via Histonet <
> > histonet@lists.utsouthwestern.edu> wrote:
> >
> >> Hi Melissa
> >> This is true, and a source of my neverending frustration with NYS Office
> >> of Professions. Stephanie Shulman (stephanie.shul...@health.ny.gov) is
> a
> >> good person at NYS to get clarification, but she will reiterate the
> same.
> >>
> >> NYS has explained to me that "histotechs don't exercise judgment" and
> thus
> >> are not qualified to take on the role of supervisor, even for a
> >> histology-only laboratory. The supervisors here in my lab and myself all
> >> have Clinical Lab Technologist licenses, from when they were
> grandfathered
> >> back in 2007. I have techs who went to school specifically for
> Histology,
> >> but can never become supervisors. I don't know what anyone will do for
> the
> >> next generation of supervisors - your situation is a real fear.
> >>
> >> Anyone in NYS who would like to raise this issue with your
> >> representatives, I encourage you to do so!!
> >>
> >> Alison Perl, HTL(ASCP)CM
> >> Anatomic Pathology Manager
> >> CareMount Medical
> >> (914) 302-8424
> >> ap...@cmmedical.com
> >>
> >>
> >> -Original Message-
> >> From: Melissa Owens via Histonet [mailto:
> histonet@lists.utsouthwestern.edu]
> >>
> >> Sent: Thursday, February 21, 2019 11:21 AM
> >> To: histonet@lists.utsouthwestern.edu
> >> Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology
> >> Supervisor
> >>
> >> Hello,
> >>
> >> I have a question about the requirements to be a supervisor in New York.
> >> New York dept. of Ed states qualifications for a Medical
> >> Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology
> >> Supervisor but no where does it mention qualifications for a
> >> Histototech/Histology Supervisor. So therefore, I have heard that in New
> >> York, a Histology Supervisor must qualify under the Medical
> >> Technologist/Laboratory Supervisor qualifications statues. This seems
> >> impossible to me as this would have to be an individual who becomes a
> >> Medical Technologist and by random transfers into a histology role then
> >> becomes a supervisor. Does anyone out there in New York legally qualify
> >> your histology supervisors who have a bachelors degree but were not
> former
> >> Medical Technologists? Thank you for any help as I have intensely scoped
> 

Re: [Histonet] [EXTERNAL] Re: New York Qualifications for Histology Supervisor

[Colleen Forster via Histonet]

YEs, it isI have done it and it is a heavy load...and in this case
completely unnecessaryshe is qualified without having to be a Med
Tech...goodness

C

On Thu, Feb 21, 2019 at 11:07 AM Perl , Alison  wrote:

> Fortunately our company has tuition reimbursement, so one of my techs is
> going to school for Med Tech part time (despite her degree in Histology) so
> that she has a future in this industry. The others are not so ambitious at
> the moment – it’s a lot to work FT and got to school 2-3 nights a week!
>
>
>
> *Alison Perl, HTL(ASCP)CM*
> * Anatomic Pathology Manager *
>
> (914) 302-8424
> *ap...@cmmedical.com* 
>
>
>
> *From:* Colleen Forster [mailto:cfors...@umn.edu]
> *Sent:* Thursday, February 21, 2019 11:59 AM
> *To:* Perl , Alison
> *Cc:* Melissa Owens; histonet@lists.utsouthwestern.edu
> *Subject:* [EXTERNAL] Re: [Histonet] New York Qualifications for
> Histology Supervisor
>
>
>
> Wow...this is just crazy~ The regulations leave histology in a real tough
> spot...
>
>
>
> Feeling for all of you and yes, for the future histology techs coming up~
>
>
>
> Colleen Forster
>
> U of MN
>
>
>
> On Thu, Feb 21, 2019 at 10:52 AM Perl , Alison via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> Hi Melissa
> This is true, and a source of my neverending frustration with NYS Office
> of Professions. Stephanie Shulman (stephanie.shul...@health.ny.gov) is a
> good person at NYS to get clarification, but she will reiterate the same.
>
> NYS has explained to me that "histotechs don't exercise judgment" and thus
> are not qualified to take on the role of supervisor, even for a
> histology-only laboratory. The supervisors here in my lab and myself all
> have Clinical Lab Technologist licenses, from when they were grandfathered
> back in 2007. I have techs who went to school specifically for Histology,
> but can never become supervisors. I don't know what anyone will do for the
> next generation of supervisors - your situation is a real fear.
>
> Anyone in NYS who would like to raise this issue with your
> representatives, I encourage you to do so!!
>
> Alison Perl, HTL(ASCP)CM
> Anatomic Pathology Manager
> CareMount Medical
> (914) 302-8424
> ap...@cmmedical.com
>
>
> -Original Message-
> From: Melissa Owens via Histonet [mailto:histonet@lists.utsouthwestern.edu]
>
> Sent: Thursday, February 21, 2019 11:21 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology
> Supervisor
>
> Hello,
>
> I have a question about the requirements to be a supervisor in New York.
> New York dept. of Ed states qualifications for a Medical
> Technologist/Laboratory Supervisor and a Cytotechnologist/Cytology
> Supervisor but no where does it mention qualifications for a
> Histototech/Histology Supervisor. So therefore, I have heard that in New
> York, a Histology Supervisor must qualify under the Medical
> Technologist/Laboratory Supervisor qualifications statues. This seems
> impossible to me as this would have to be an individual who becomes a
> Medical Technologist and by random transfers into a histology role then
> becomes a supervisor. Does anyone out there in New York legally qualify
> your histology supervisors who have a bachelors degree but were not former
> Medical Technologists? Thank you for any help as I have intensely scoped
> the New York department of Education website in my frustrations.
>
> By the way, I am asking this because I have a Day Shift 7:30am-4pm
> Monday-Friday Histology Supervisor position in the New York Metropolitan
> area with these requirements and I don't know if there is a way around
> them. Thank you!
>
> Melissa Owens, CHP (ASA)
> President, Laboratory Staffing
> Allied Search Partners
>
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> This email and its attachments, if any, may contain confidential or
> proprietary information and are intended solely for authorized use by the
> intended recipient(s) only. Any other use of this email is prohibited. If
> you have received this email in error, you are hereby notified that any
> retention, disclosure, copying, forwarding, distribution (in whole or in
> part and whether electronically, written and/or orally) and/or taking of
> any action in reliance on this email, its contents and/or any attachments
> thereto is strictly prohibited. If you received this email in error, please
> notify the sender by replying to this message and permanently delete this
> email, and any attachments thereto, from your system immediately.
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> --
>
> Colleen Forster HT(ASCP)QIHC
>
> BLS Histology and IHC Laboratory
>
> B173 PWB  612-626-1930
>
>
>
> *If submitting histology request 

Re: [Histonet] [EXTERNAL] Re: New York Qualifications for Histology Supervisor

[Perl , Alison via Histonet]

Fortunately our company has tuition reimbursement, so one of my techs is going 
to school for Med Tech part time (despite her degree in Histology) so that she 
has a future in this industry. The others are not so ambitious at the moment – 
it’s a lot to work FT and got to school 2-3 nights a week!

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
(914) 302-8424
ap...@cmmedical.com

From: Colleen Forster [mailto:cfors...@umn.edu]
Sent: Thursday, February 21, 2019 11:59 AM
To: Perl , Alison
Cc: Melissa Owens; histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] New York Qualifications for Histology 
Supervisor

Wow...this is just crazy~ The regulations leave histology in a real tough 
spot...

Feeling for all of you and yes, for the future histology techs coming up~

Colleen Forster
U of MN

On Thu, Feb 21, 2019 at 10:52 AM Perl , Alison via Histonet 
mailto:histonet@lists.utsouthwestern.edu>> 
wrote:
Hi Melissa
This is true, and a source of my neverending frustration with NYS Office of 
Professions. Stephanie Shulman 
(stephanie.shul...@health.ny.gov) is a 
good person at NYS to get clarification, but she will reiterate the same.

NYS has explained to me that "histotechs don't exercise judgment" and thus are 
not qualified to take on the role of supervisor, even for a histology-only 
laboratory. The supervisors here in my lab and myself all have Clinical Lab 
Technologist licenses, from when they were grandfathered back in 2007. I have 
techs who went to school specifically for Histology, but can never become 
supervisors. I don't know what anyone will do for the next generation of 
supervisors - your situation is a real fear.

Anyone in NYS who would like to raise this issue with your representatives, I 
encourage you to do so!!

Alison Perl, HTL(ASCP)CM
Anatomic Pathology Manager
CareMount Medical
(914) 302-8424
ap...@cmmedical.com


-Original Message-
From: Melissa Owens via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, February 21, 2019 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] New York Qualifications for Histology Supervisor

Hello,

I have a question about the requirements to be a supervisor in New York. New 
York dept. of Ed states qualifications for a Medical Technologist/Laboratory 
Supervisor and a Cytotechnologist/Cytology Supervisor but no where does it 
mention qualifications for a Histototech/Histology Supervisor. So therefore, I 
have heard that in New York, a Histology Supervisor must qualify under the 
Medical Technologist/Laboratory Supervisor qualifications statues. This seems 
impossible to me as this would have to be an individual who becomes a Medical 
Technologist and by random transfers into a histology role then becomes a 
supervisor. Does anyone out there in New York legally qualify your histology 
supervisors who have a bachelors degree but were not former Medical 
Technologists? Thank you for any help as I have intensely scoped the New York 
department of Education website in my frustrations.

By the way, I am asking this because I have a Day Shift 7:30am-4pm 
Monday-Friday Histology Supervisor position in the New York Metropolitan area 
with these requirements and I don't know if there is a way around them. Thank 
you!

Melissa Owens, CHP (ASA)
President, Laboratory Staffing
Allied Search Partners



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If submitting histology request please also forward to Lori Holm at 
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Re: [Histonet] [External Sender] Re: Closed doors in Histology

[Hannen, Valerie via Histonet]

We have kept our doors closed at all times for years due to the negative 
pressure issue.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com




-Original Message-
From: Morken, Timothy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, November 16, 2018 11:59 AM
To: Histonet
Subject: [External Sender] Re: [Histonet] Closed doors in Histology

WARNING: This message came from an external source. Please do not click links 
or open attachments if unexpected or unusual.

That'll be tough for our histo lab - there are no doors. It is open to two 
hallways and adjacent clin lab. That said, we have so many hoods in there that 
we probably make the entire floor negative pressure! And there is no odor from 
the lab.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


-Original Message-
From: Mike Pence via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Friday, November 16, 2018 8:23 AM
To: histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwestern.edu
Subject: [Histonet] Closed doors in Histology

OK, How many of you keep your doors to your Histology Dept. closed all the 
time? We were told by a CIHQ inspector that Histology Dept must be under 
negative air flow ALL THE TIME. This was a new one for me. The standard is from 
the ASHRAE 170 table 7.1
This is just an FYI for you all that these are the kinds of things CMS is 
looking at now days.

Michael S. Pence | Supervisor of Laboratory Services
Great River Health Systems
1221 S. Gear Ave. | West Burlington, IA 52655
Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
mpe...@grhs.net | 
www.greatrivermedical.org.
www.Facebook.com/GreatRiverHealthSystems
 | www.Twitter/GreatRiverMed


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Re: [Histonet] [EXTERNAL] Histonet Digest, Vol 180, Issue 1

[Bummer, Katherine via Histonet]

Hello Allan,

I have been seeing the same thing but with HistoClearII.  I have been using 
alkaline phosphatase substrates on some double chromogenic staining that 
requires me to either dehydrate and clear with a non-Xylene solvent or just 
mount with aqueous PVP mounting media.  I had thought that it was perhaps some 
H2O carrying over into my dehydration and clearing but never did resolve fully. 
 Any info that comes of this I would like to be included on as well.

Thank you.

Kate

-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu 
 
Sent: Thursday, November 1, 2018 10:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Histonet Digest, Vol 180, Issue 1

Send Histonet mailing list submissions to
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Re: [Histonet] [EXTERNAL] Re: Pinning Gross Specimens

[Porter, Douglas via Histonet]

Bryan's advice is spot on.  We do the same thing and cut the paraffin into 
sizes we need.
With some specimens, you can pin them down and return them to the containers 
they arrived in.
Provided they arrive in containers appropriate for the size of the 
specimen...wink, wink!!

Douglas A. Porter, HT (ASCP)
Pathologist Assistant
Anatomic Pathology IT Coordinator

Sparrow Center for Laboratory Medicine
Department of Pathology
3392 Patient Care Drive
Lansing, MI 48911

517-371-9481 (phone)
517-371-9540 (fax)

douglas.por...@sparrow.org



-Original Message-
From: Bryan Llewellyn via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, November 01, 2018 1:41 PM
To: White, Lisa M.; histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Pinning Gross Specimens

 Warning: This email originated from outside of Sparrow. Do not click links or 
open attachments unless you recognize the sender  and are expecting the message.

--
If you can't find suitable pin out boards, they can be made easily
enough. Obtain some suitably sized plastic containers with lids and fill
them an inch deep with used, filtered, paraffin wax that would otherwise
be discarded. After using, just remelt, filter if needed, then harden again.

Bryan Llewellyn


White, Lisa M. via Histonet wrote:
> Hello all,
>
> Does anyone know of a container with lid that has cork in the bottom?  This 
> would be utilized to pin out gross specimens for fixation.  If so can you 
> send me vendor info?
>
> Lisa White, HT(ASCP)
> James H. Quillen VAMC
>
> ___
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Re: [Histonet] [External Sender] Cell Block

[Hannen, Valerie via Histonet]

We use equal amounts of drops.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-Original Message-
From: White, Lisa M. via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, April 25, 2018 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External Sender] [Histonet] Cell Block

WARNING: This message came from an external source. Please do not click links 
or open attachments if unexpected or unusual.

Does anyone have the recipe for making cell blocks with Thrombin and Plasma?
We made them at my first Histo job, oh say over 20 years ago but I do not 
remember the ratio.

Thanks bunches,
Lisa

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Re: [Histonet] [EXTERNAL] Decal stat

[Samantha Golden via Histonet]

For bone marrow specimens you want to use immunocal, a formic acid based 
product. This will help preserve tissue antigenicity when performing IHC and 
ISH staining.

Get Outlook for iOS

From: Randa Nasr via Histonet 
Sent: Friday, April 20, 2018 2:54:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] Decal stat

Hi,

We are currently using Decal from Richard Allan for BM calcification for
about an hour, we are considering switching to Decal stat from StatLab
which is supposed to work in 10 minutes, does anyone currently uses the
Decal stat and have any feedback on it?


Regards,


*Randa Jannoun-Nasr, MD*

Histology & Molecular Lab Technical Director

[image: siP - www.siparadigm.com] 

*d*
*m*
*f*
+1 888-599-5227 x4041
+1 732-991-8230
+1 201-599-9066
25 Riverside Dr, Ste 2
Pine Brook, NJ 07058
United States
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Re: [Histonet] [External]: Re: Slide labels falling off- Slidemate AS

[Harris, Stefanie via Histonet]

Hi Laurie,

   Thanks for you response. I am talking about the print from the slidemate, 
not paper labels.


We have tried different lots and it seems to be a bunch of them.  There was one 
lot that was really bad in December and we thought that would be the end of the 
problem. We contacted the vendor and they said they hadn't heard of any other 
tissues and have made no changes. We can see the variability in some of the 
white frosted area so there are clearly differences in the slide.  When we 
contacted the 2nd time about the ongoing issue, they said that the slides were 
not tested for use on that printer but they would look into it.


I have a couple different brands (Fisher & Statlab) that I am hoping to test 
out but I am open other suggestions as well.

Stefanie Harris
Research Assistant, Histology
Research Animal Diagnostic Services | Charles River
261 Ballardvale St., Wilmington, MA 01887
P: 781.222.6592 | F: 978.658.7698
stefanie.har...@crl.com | www.criver.com
LinkedIn | 
Twitter | 
Facebook | 
Eureka

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received this email in error, please notify the sender immediately by email or 
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From: Laurie Colbert 
Sent: Wednesday, March 28, 2018 12:01:36 PM
To: Harris, Stefanie; histonet@lists.utsouthwestern.edu
Subject: [External]: Re: [Histonet] Slide labels falling off- Slidemate AS

Stefanie,

When you say "labels" are  you talking about paper labels or the print on the 
slides from the Slidemate??

I will assume that you mean the print, and yes, I have had the problem in the 
past where the print came off during staining and/or you could literally wipe 
the print off with a kimwipe or your finger.  In our case, it was the slides - 
even though it was the same slide we had been using for years.  This was 
happening with a certain lot number.  Is this happening with just one lot 
number?  Maybe you can try and get a different lot number or try a totally 
different slide (different manufacturer) to narrow down the issue.
Good luck!
Laurie



-Original Message-
From: Harris, Stefanie via Histonet 
To: histonet 
Sent: Wed, Mar 28, 2018 8:51 am
Subject: [Histonet] Slide labels falling off- Slidemate AS

Hello all,

I am hoping someone out there can help. We have been having issues with some of 
the labels coming off during staining and after. It seems to be random so I 
can't tell if there is a problem with the slides or the Slidemate AS. There are 
days that it will be half the slides but other days where it will be none. 
Sometimes the labels peel off and float away during staining and other times 
labels rub off when handling but only after staining.


We are using Globe Scientific Slides, and have been for over 2 years with no 
issues until December.

I have changed out the ribbon on the slidemate and the problem is still 
sporadic.

Oven time drying time does not seem to be a factor.

We have not made any reagent changes for the staining process to explain the 
issue either.


Has anyone else seen anything like this? Any slide suggestions to try out?

Stefanie Harris
Research Assistant, Histology
Research Animal Diagnostic Services | Charles River
261 Ballardvale St., Wilmington, MA 01887
P: 781.222.6592 | F: 978.658.7698
stefanie.har...@crl.com | 
www.criver.com>
LinkedIn>
 | 

Re: [Histonet] [EXTERNAL] Deplastifying MMA

[Dorothy Hu via Histonet]

Hi Lori,

Would you please tell me which company and catalog # of  tetrahydrofuran
you used before?
For how many minutes you did for deplastifying in tetrahydrofuran?
Thanks in advance.

Dorothy

Message: 3
Date: Wed, 14 Feb 2018 20:05:16 +
From: "Garcia, Lori, M.Sc." 
To: "reuel.corne...@tsrh.org" 
Cc: "histonet@lists.utsouthwestern.edu"

Subject:
Message-ID:


Content-Type: text/plain; charset="us-ascii"

Hi Reuel,

One thing you could try is THF (tetrahydrofuran). We have used it in the
past to rapidly dissolve the MMA on slides.

Good luck,
Lori
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Re: [Histonet] [EXTERNAL] Deplastifying MMA

[Garcia, Lori, M.Sc. via Histonet]

Hi Reuel,

One thing you could try is THF (tetrahydrofuran). We have used it in the past 
to rapidly dissolve the MMA on slides.

Good luck,
Lori

-Original Message-
From: Dorothy Hu via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 14, 2018 11:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] [Histonet] Deplastifying MMA

>
> Hi  Reuel
>

We had the same thing as you. It was caused by bad MMA, I tested. Write down 
the lot # and ask refund. Test new lot # 1st of MMA in the future. It was very 
frustratingI know.
Best luck.

Dorothy





> Message: 2
> Date: Mon, 12 Feb 2018 21:07:15 +
> From: Reuel Cornelia 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] Deplastifying MMA
> Message-ID:
>  namprd17.prod.outlook.com>
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Hello histonetters,
>
> I have a problem with my MMA plastic section on my slide. I could not
> remove the plastic MMA even if I leave the section slides in Xylene
> for several days with heat incubation at 60 degrees. This is the first
> time that happened to my section for several years of doing the same thing 
> over.
> I was thinking that it was my Xylene lot number so I tried to change
> it but still does not remove the plastic, and then Itried different
> solvent Acetone, and Ethylene glycol Monoethyl ether but still it does not 
> work.
> Can I ask for help if anyone knows what was going on and what would be
> the best way to remove this plastic from my section?
>
> Just to give you my embedding solution was MMA -94% , dibutly
> phthalate- 5% and perkadox 16- 0.5%. This have been my solution for
> years and I do not have any problem with the removal of plastic
> section until now. I was thinking that my MMA (M55909) different lot
> number from Sigma Aldrich may have cause this because even I tried to
> use the MMA to dissolve my plastic it does not work.
>
>
> Thank you for and any opinions or protocols are greatly appreciated.
>
>
> Reuel Cornelia
>
> TSRH
>
> 214-559-7766
>
>
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[Histonet] External Quality Assurance Programs

[Tom Wells via Histonet]

Hi,
Some time ago I discovered an incredibly useful teaching resource. I believe it 
was part of an external quality assurance program for Histopathology. It had a 
large collection of images from materials that they had received for 
evaluation. These images were from all areas such as Immunohistochemistry, 
special stains, H, etc. They included ratings that they had given them as 
feedback. Including all mistakes that they had observed. Unfortunately, I 
didn't keep track of the site and I can no longer find it. If anyone can recall 
seeing this site I would appreciate it since it would take a lifetime to 
deliberately recreate all of the mistakes. Thanks. Tom

Tom Wells BSc, MEd, MLT, ART
Faculty
Medical Laboratory Science
School of Health Sciences
SW03-3088
(604) 412-7594
BCIT

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Re: [Histonet] [External Sender] Joint Commission

[Hannen, Valerie via Histonet]

Our grossing area is in a negative pressure room due to the formalin, alcohol 
and xylene fumes.


Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com



-Original Message-
From: raestask via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, February 07, 2018 11:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [External Sender] [Histonet] Joint Commission

WARNING: This message came from an external source. Please do not click links 
or open attachments if unexpected or unusual.

Has anyone heard of a regulation by the Joint Commission to require grossing be 
done in a negative pressure room?
Rae StaskiewiczUnity point Health MethodistPeoria, Il

Sent from my Verizon, Samsung Galaxy smartphone 
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Re: [Histonet] [External Sender] Grossing qualifications

[Hannen, Valerie via Histonet]

No. 

Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.han...@parrishmed.com
www.parrishmed.com




-Original Message-
From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, January 22, 2018 8:24 AM
To: Histo List
Subject: [External Sender] [Histonet] Grossing qualifications

WARNING: This message came from an external source. Please do not click links 
or open attachments if unexpected or unusual.

Do histotechs and or histotechnicians qualify to do high complexity grossing 
without a pathologist or PA assistance?

An example would be a large breast ressection.

--

Charles Riley BS  HT, HTL(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] [External] Histonet Digest, Vol 170, Issue 13

[Arrington, Karla A via Histonet]

Histology opening in Anchorage, Alaska


ATTENTION HISTOLOGISTS! Do you know of anyone who is looking for a job? We have 
an opening at Alaska Native Medical Center, pathology department located in 
Anchorage, Alaska.  It is a great opportunity for someone who is HT certified.

Duties to include (but not limited to): Accessioning, data entry into our 
SoftPath system, embedding, microtomy, manual special stains, IHC, preparations 
of Non-Gyn specimens on ThinPrep unit, general histology and histology related 
clerical duties. The right candidate is reliable, self-motivated and dependable.

Full Time Days, Monday through Friday with NO WEEKENDS!  We offer a benefits 
package including medical, dental and vision insurance, paid holidays, 
vacation, sick accrual and a profit sharing 401K plan.  Salary is negotiable 
depending on experience. If you are interested, or if you know of someone, 
contact me and I will give you more details.



Karla Arrington

Karla Arrington, HT (ASCP) HIT (AHIMA) BS
Supervisor of Pathology
Alaska Native Medical Center
Tele: 907-729-1810
Fax: 907-729-1226
 kaarring...@anthc.org

From: histonet-requ...@lists.utsouthwestern.edu 

Sent: Sunday, January 14, 2018 9:00:02 AM
To: histonet@lists.utsouthwestern.edu
Subject: [External] Histonet Digest, Vol 170, Issue 13

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Today's Topics:

   1. Re: Histology Hacks (Hobbs, Carl)
   2. Re: Cross Contamination CAP policy (Bob Richmond)
   3. Histology Opening in Beautiful Monterey, CA (Eileen Akemi Allison)
   4. Histology Opening in Beautiful Monterey, CA (Eileen Akemi Allison)


--

Message: 1
Date: Sat, 13 Jan 2018 19:09:33 +
From: "Hobbs, Carl" 
To: histonet 
Subject: Re: [Histonet] Histology Hacks
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

I thought Histonet was a primary source of "hacks"?
Obviously...not.
I have many hacks, as many others do, too...
Have to admit that Histonet is rather Conservative/lazy

Eg: In Imagesthere's a potential MASSIVE catalogue so, why do you not add 
your images to the Histonet archive???
Histonet image archive should be better/bigger than ANY other Dye/IHC/IF 
archive?

It should be, as is HPA/Gensat/Allen , a REFERENCE
Sigh, it is not.
yet.
?Or , I am wrong?
Please tell me
Carl









--

Message: 2
Date: Sat, 13 Jan 2018 14:32:45 -0500
From: Bob Richmond 
To: "Histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Cross Contamination CAP policy
Message-ID:

Content-Type: text/plain; charset="UTF-8"

Terri L. Braud, HT(ASCP), Anatomic Pathology Supervisor at Holy Redeemer
Hospital in Meadowbrook PA notes:

>>Our policy calls for wiping of forceps with gauze between cases at gross
and at embedding. At gross, we use a disposable absorbent lined pad on the
cutting board for each larger case, and just a fresh c-fold paper towel
between small biopsy cases. We do not allow double-dipping of swabs into
ink, but instead, pour out small amounts into a large plastic weigh boat
which is also discarded after the case. We use disposable safety scalpels,
with a 70 blade (love 'em) for each case. For excessively bloody/fatty
cases, we put the dirty forceps into an enzyme pre-soak (Aseptizyme) to
remove all tissue debris. Then they are scrubbed with a brush, then rinsed
in a disinfectant before being re-used.<<

I've never seen a pathology service (and I've worked in 80 of them) do any
of these things, all of them good ideas. I'm glad to see the CAP taking the
issue up. Carry-overs from case to case are common, particularly when the
grosser is overworked and working too fast. I've never seen a serious error
made as a result of such contamination, but I've seen a few close calls.

Terri Braud, could you copy us the actual text in the CAP inspection form?

Bob Richmond
Samurai Pathologist

Re: [Histonet] [EXTERNAL] ICD-10 Codes

[Lester Raff MD via Histonet]

Interesting--we always require and ICD from our submitting providers even 
though we are all part of the same group.

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203


-Original Message-
From: Megan Dishop via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, April 25, 2017 9:28 AM
To: Richard Cartun; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] [EXTERNAL] ICD-10 Codes

I don't think that is necessary.  Pathology services are coded by the 
pathologic diagnosis, unlike laboratory testing that requires an ICD10 code or 
reason for test request upfront.  The blog entry below mentions this 
distinction

http://www.mckesson.com/bps/blog/preparing-for-icd-10-by-addressing-incomplete-diagnosis-information-received-errors/


Megan K. Dishop MD
Medical Director, Pediatric Anatomic Pathology Children's Hospitals and Clinics 
of Minnesota Laboratories
2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA
Phone: 612-813-6521Fax: 612-813-7721  Email: megan.dis...@childrensmn.org
Adjoint Professor of Pediatrics, University of Colorado School of Medicine




>>> "Cartun, Richard via Histonet" <histonet@lists.utsouthwestern.edu> 
>>> 4/25/2017 9:02 AM >>>
What do you do when you receive a pathology consult and it is missing an ICD-10 
code?  I am being told that we cannot accession it until we get a code from the 
submitting doctor's office, and that we cannot add one.  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory Director, Biospecimen Collection Programs Assistant 
Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] [EXTERNAL] ICD-10 Codes

[Megan Dishop via Histonet]

I don't think that is necessary.  Pathology services are coded by the 
pathologic diagnosis, unlike laboratory testing that requires an ICD10 code or 
reason for test request upfront.  The blog entry below mentions this 
distinction

http://www.mckesson.com/bps/blog/preparing-for-icd-10-by-addressing-incomplete-diagnosis-information-received-errors/


Megan K. Dishop MD
Medical Director, Pediatric Anatomic Pathology
Children's Hospitals and Clinics of Minnesota Laboratories
2525 Chicago Ave S. MS32-B600, Minneapolis, MN 55404 USA
Phone: 612-813-6521Fax: 612-813-7721  Email: megan.dis...@childrensmn.org
Adjoint Professor of Pediatrics, University of Colorado School of Medicine




>>> "Cartun, Richard via Histonet"  
>>> 4/25/2017 9:02 AM >>>
What do you do when you receive a pathology consult and it is missing an ICD-10 
code?  I am being told that we cannot accession it until we get a code from the 
submitting doctor's office, and that we cannot add one.  Thank you.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax


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Re: [Histonet] [EXTERNAL] Re: Tissue fixation

[Elizabeth Chlipala via Histonet]

I'm going to give my two cents here.  I think you need to look closely at how 
you handle these samples.  First of all leeps are not small samples therefore 
just because the leep has been placed in fixative upon removal and then sits in 
fixative until its processed.  Unless the sample is grossed  into smaller 
pieces only then it will adequately fix.   Electro cautery  instruments 
function to do two things, they cut and then cauterize - there is always a 
thermal defect associated with the process or thermal spread, this is clearly 
evident on the H slides.   The process is essentially coagulating the tissue 
proteins.  The thermal spread and char that is associated with this process 
varies dependent upon the instrument and setting that is being used.  This 
process may decrease fixative penetration so it's important that these samples 
are grossed in as soon as possible.  Trimming the tissue will allow for better 
penetration of the fixative and good fixation requires at least 4 to 6 hour
 s in formalin AFTER the tissue has been trimmed in.The artifact that the 
pathologist is seeing might not be related to the amount of time the sample 
sits in formalin it might be due to the amount of time the samples sits  in 
formalin prior to being grossed in.

If I had this issue I would start tracking the different times associated with 
these samples and possibly the instrument and setting used (if you are able to 
get that info).   

1.  Time to fixative - cold ischemic time
2.  Time to when the sample is grossed in 
3.  Time in formalin once the sample is grossed in

Good Luck I hope this helps.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Stedman, Nancy via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 31, 2017 12:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] [EXTERNAL] Re: Tissue fixation

I would bet there are quite a few pathologists on this mailing list (like me) 
who are here because we respect histo techs and know you have valuable 
information to offer!

I agree, cautery artifact is pretty easy to tell from poor fixation, so it 
should be easy to determine if that is the problem.  Sometimes, specimens that 
are submitted smashed in a cassette may have poor fixation of the tissue in 
contact with the cassette.  Or if they are submitted sponged and floating, some 
tissue may not be fully immersed in formalin.  Also, crush artifact may 
resemble poor fixation, so maybe the clinical team is not handling the tissue 
gently enough?  

I read veterinary specimens; we don't do LEEPs so I don't know if any of these 
scenarios are possible, but they are things I see frequently.  

-Nancy Stedman


-Original Message-
From: Mayer,Toysha N via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 31, 2017 2:15 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL] Re: [Histonet] Tissue fixation

I agree with Rene.  To discredit the pathologist theory  show if all of the 
specimens from the run are not fixed properly.  Then show if it is just the 
LEEPs.  Then show if it is that particular clients specimens?  Then onto that 
client's LEEPs.
That should prove your problem lies with the client handling and not the 
fixation on your end.  Cauterization can cause burning of the specimen, but not 
look unfixed. If it was left out of formalin, autolysis can set in.
The situation shows the god-complex some physicians have and the lack of 
respect they have for their techs.  
This is why we should all be certified and keep up with continuing education, 
so that these pathologists will respect us.
Ok, I'm going to get off my soapbox now, before I say something ugly.

Toysha Mayer






--

Message: 3
Date: Fri, 31 Mar 2017 12:50:37 +
From: T H <thiggin...@msn.com>
To: "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Subject: [Histonet] Tissue Fixation
Message-ID:

<sn1pr19mb060735d7b896c67b0223e098d8...@sn1pr19mb0607.namprd19.prod.outlook.com>

Content-Type: text/plain; charset="iso-8859-1"

Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our cl

Re: [Histonet] [EXTERNAL] Re: Tissue fixation

[Stedman, Nancy via Histonet]

I would bet there are quite a few pathologists on this mailing list (like me) 
who are here because we respect histo techs and know you have valuable 
information to offer!

I agree, cautery artifact is pretty easy to tell from poor fixation, so it 
should be easy to determine if that is the problem.  Sometimes, specimens that 
are submitted smashed in a cassette may have poor fixation of the tissue in 
contact with the cassette.  Or if they are submitted sponged and floating, some 
tissue may not be fully immersed in formalin.  Also, crush artifact may 
resemble poor fixation, so maybe the clinical team is not handling the tissue 
gently enough?  

I read veterinary specimens; we don't do LEEPs so I don't know if any of these 
scenarios are possible, but they are things I see frequently.  

-Nancy Stedman


-Original Message-
From: Mayer,Toysha N via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, March 31, 2017 2:15 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [EXTERNAL] Re: [Histonet] Tissue fixation

I agree with Rene.  To discredit the pathologist theory  show if all of the 
specimens from the run are not fixed properly.  Then show if it is just the 
LEEPs.  Then show if it is that particular clients specimens?  Then onto that 
client's LEEPs.
That should prove your problem lies with the client handling and not the 
fixation on your end.  Cauterization can cause burning of the specimen, but not 
look unfixed. If it was left out of formalin, autolysis can set in.
The situation shows the god-complex some physicians have and the lack of 
respect they have for their techs.  
This is why we should all be certified and keep up with continuing education, 
so that these pathologists will respect us.
Ok, I'm going to get off my soapbox now, before I say something ugly.

Toysha Mayer






--

Message: 3
Date: Fri, 31 Mar 2017 12:50:37 +
From: T H 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Tissue Fixation
Message-ID:



Content-Type: text/plain; charset="iso-8859-1"

Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.  She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).  She claims we must be diluting our formalin to cause this 
issue or "something".  We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some back ground on how our process works.  Our clients send us 
all our specimens to us via Overnight FedEx or UPS in 10% formalin they will 
then they sit in 10% formalin in-house until the processors are started around 
3pm and sits an additional 5 hours in 10% formalin on the processor before the 
processor actually starts.  That being said the fixation process has had a 
pretty good start before we ever even touch it.


My question is, and I thought I had read this in the past is, when a specimen 
is left out prior to fixation and lying on a absorbent surface such as a paper 
towel, won't the area of the tissue touching the absorbent surface begin the 
disintegration processes faster in that exact area then the rest of the 
specimen?  Or if you have any other suggestion on what might be happening to 
only "certain" specimens would be great as well.


Thanks for your help!


Tim



--

Message: 4
Date: Fri, 31 Mar 2017 13:32:35 + (UTC)
From: Rene J Buesa 
To: T H ,   "histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] Tissue Fixation
Message-ID: <1730848134.8065268.1490967155...@mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8

What you describe as a possible scenario is absolutely possible.If your PT does 
not "want to hear" about it, suggest she gets a "hearing aid" or to study 
something about histotechnology or even better yet, pay attention to what a 
professional on the subject (you) has to say about it. You would never dare to 
question her diagnosis, why would she question yours on this subject?Ren? 

On Friday, March 31, 2017 9:11 AM, T H via Histonet 
 wrote:
 

 Good Morning,


I have a pathologist that is not happy with the fixation on some of our LEEP 
specimens.? She swears its histology doing something to the specimen to cause 
the tissue to look unfixed on only "part" of the LEEP specimens (all the same 
client specimens).? She claims we must be diluting our formalin to cause this 
issue or "something".? We mentioned maybe it was on the clients end not placing 
them in 10% formalin right away, she wouldn't hear of it.


Let me give you some 

[Histonet] External alarm system for VIP 6

[Fortune, James A. via Histonet]

Hello all,

We are looking to purchase an external alarm system for our 2 vip 6 processors. 
 I called Sakura  to see if they sold them or if they had any recommendations 
as far as which ones are compatible with the VIP and got nowhere.  So I am 
asking all of the histonet world for any recommendations on external alarms you 
have used or purchased.  Thanks in advance!

James (Andy) Fortune
Histology Tech Specialist
Meriter Health Services
Madison, WI
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Re: [Histonet] [External] Re: Automated Slide Stainers

[Bitting, Angela K. via Histonet]

There are slide basket inserts to accommodate that sized slide for Sakura 
stainers. The DRS2000 for example. Check out this web page.

http://www.tedpella.com/histo_html/slides-large.htm


-Original Message-
From: Atoska Gentry via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Friday, August 05, 2016 11:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [External] Re: [Histonet] Automated Slide Stainers

Hello, one of my department's researchers is in need of an automated slide 
stainer which accommodates 3"x2" microscope slides. If you have experience with 
such will you please contact me at your earliest convenience? Regards, Atoska 
Gentry ___
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Re: [Histonet] [External] Crystals in PAS from Ventana

[Bitting, Angela K. via Histonet]

Hi Mark,
 We still have this issue after two years of complaining to Roche.  We've had 
multiple TAS's come in and we've sent slides to Tucson. They've gone over the 
instruments, etc. Long story short, their fix was adding something to their 
package insert that says "Schiff's Reagent is known to contain some needle-like 
precipitate. At expected levels, this precipitate should not affect assay 
performance". Good luck.

-Original Message-
From: Mark, Danielle via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Wednesday, July 20, 2016 12:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Crystals in PAS from Ventana

I am wondering if anyone else has been having issues with the PAS stain.  The 
crystals are not rinsing off of the slide using the benchmark special stainer.  
We are on our 3rd lot number, no change.  We have had our tubing changed and 
our instrument checked to make sure the rinse is appropriate.  This problem is 
causing our lab great frustration.  Any feedback would be appreciated.


Danielle Mark, HT(ASCP), Technical Specialist ACL Laboratories
8901 W Lincoln Ave. | West Allis, WI 53227
O: 414.328.6235 | F: 414.328.6237 |
danielle.m...@aurora.org

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Re: [Histonet] [EXTERNAL]

[Roy, Ryan via Histonet]

Hello,

I wish you had contacted me a few months back. Management moved a tech over 
internally here.

I will put you in contact with White River Junction VAMC . They may have a 
postion open. Also, I would check University of Vermont Med center;, 
Brattleboro Memorial hospital, Dartmouth Medical Center, Catholic Medical 
Center, Manchester.

I know people at all these locations, often times a latent position (everyone 
knows about it ) won't post until a certain period so you wouldn' t know its 
there without asking. That said, Ill ask around.


Ryan Roy HTL (ASCP)
Histology Lab
Manchester VA
718 Symth Rd
Manchester NH 03104
(603) 624-4366 ex 6640


Disclosure: The content of this email does not reflect the policies, views or 
opinions of the VA.



From: Kaylie Grenier [mailto:kgreni...@student.goodwin.edu]
Sent: Tuesday, May 17, 2016 11:42 AM
To: Roy, Ryan
Subject: [EXTERNAL]

Good morning,

My name is Kaylie Grenier, I am a histology student at Goodwin College. Kelli 
Goodkowsky gave me your email address so I can get in contact with you. I am in 
my final semester of the program and I am hoping to relocate to New Hampshire 
at the end of August and am planning on taking my HT certification as soon as I 
am eligible. I was just wondering if you knew of any potential positions up in 
NH or if you knew of anyone I could get in contact with. I will be moving to 
the New London, NH area. Thanks!

-Kaylie Grenier

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Re: [Histonet] [EXTERNAL] Re: Staples and Sutures

[Boyd, Debbie M via Histonet]

Definitely the paths/PA's responsibility.  Putting it in the histotech's court 
is like saying the car is responsible for hitting the pedestrian (not the 
driver). Crazy. We sometimes can see them when embedding but by then the tissue 
is not as pliable for easy removal without disturbing the tissue architecture.  
Margins are destroyed by the time we get the suture out.  Ninety-nine percent 
of the time you can not find the knot to cut for removal.  
Staples can be embedded so deeply that they don't "shine" in reflective light.  
Only the blade finds them. If there is a whole line of them, the tissue is 
practically destroyed by pulling them out. 

Debbie M. Boyd HT (ASCP) | Chief Histologist  | Southside Regional Medical 
Center | 200 Medical Park Blvd.  |  Petersburg, Va.  23805 | PH 804-765-5025 | 
FAX 804-765-6058


From: Bob Richmond via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, September 29, 2015 8:47 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] Staples and Sutures

Kenneth G Metzger HTL(ASCP), Histology Supervisor, ARUP Labs, Salt Lake
City, Utah asks:

>>Recently we have had a marked increase in staples and sutures in our
tissue that arrives for processing. I have tried to relay to our
pathologists and PA's the difficulty in cutting sections and the tissue
destruction in getting staples and sutures out once the tissue is processed
to paraffin. I was told by some of them that in their experience histology
should dealing with this issue. Though my own opinion is this is position
is not valid, I've been asked to collect opinions from other histology
departments if there are protocols that the grossing individual should be
removing the foreign object. Please give me any feedback...Thanks<<

What la-la land are your pathologists and PAs off in? I've caught plenty of
hell in my life in pathology since 1964 - particularly back before the days
of disposable blades - for not getting staples out of tissue before
submitting it. This is definitely the responsibility of whoever's doing the
grossing.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: [Histonet] [EXTERNAL] Re: competency assessment for Anatomic Pathologist

[Boyd, Debbie M]

Our pathologist are evaluated using the OPPE ( Ongoing Physician Performance 
Evaluation) method.  In that method we monitor TAT for Surg. Path, Cyto, Autop. 
Frozen Sections, Bone Marrows.  Cyto to Histo correlations, frozen to permanent 
correlations, Post OP to Final correlations.  Peer Review cases agreements vs. 
disagreements.
The below information is formated on an Excel spreadsheet.  This information is 
reported quarterly to our QA department.  They take care of the rest for 
re-credentialing.


Cases Reviewed
Frozen Section/Histology Correlation
Cytology/Histology Correlation
Autopsies 
Preliminary Autopsy Report completed within 72 hrs
Final Autopsy Report completed within 60 Days
Bone Marrow Aspiration Reviews 
INTERNAL REVIEWS 
  
  External Consultations
1-No difference in interpretations
2-Difference in interpretationw w/no potential impact
3-Difference in interpretation w/minimal potential impact
4-Difference in interpretation w/significant potential for altering plan of care

Surgical Turnaround Study- #Cases Sampled
24 hours
48 hours
72 hours
Decals, BM, Send Outs  96 hours
Percentage Completed Within 48 hours

CytologyTurnaround Study- #Cases Sampled
24 hours
48 hours
72 hours
96 hours
Percentage Completed Within 48 hours



Debbie M. Boyd HT (ASCP) | Chief Histologist  | Southside Regional Medical 
Center | 200 Medical Park Blvd.  |  Petersburg, Va.  23805 | PH 804-765-5025 | 
FAX 804-765-6058


From: S hay [susanha...@gmail.com]
Sent: Saturday, June 20, 2015 11:10 PM
To: Jamal
Cc: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] competency assessment for Anatomic 
Pathologist

Please post here. I am interested as well.
On Jun 20, 2015 9:21 AM, Jamal j.rowa...@alborglaboratories.com wrote:

 Dear colleagues

 I hope if any one share me the form of:

 competency assessment for Anatomic Pathologist





 Best Regards,





 Jamal M. Al Rowaihi  Anatomic Pathology Supervisor   | Al Borg
 Medical Laboratories |  Mobile +966 503629832|
 mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com

 Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA|
 Phone: +966 12 670 0099   | Fax: +966 12 676 4984 |
 http://www.alborglaboratories.com/ www.alborglaboratories.com



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Re: [Histonet] [EXTERNAL] Re: plants in the lab

[Tony Henwood (SCHN)]

I agree with Paula.

The way you have explained the regs implies that even I would have problems 
entering the labs.
Maybe ban people altogether and then we meet the regs.
Lets keep it real and not fantasy.

Tony

From: Boyd, Debbie M [dkb...@chs.net]
Sent: Thursday, 4 June 2015 9:22 PM
To: Paula Pierce; Goins, Tresa; Histonet
Subject: Re: [Histonet] [EXTERNAL] Re:  plants in the lab

There is a plethora of Joint Commission regulations concerning the below 
mentioned points of entry for microbacterial spores. If you are Joint 
Commissioned inspected, plants are not allowed in the lab.  We are JC inspected 
and are not even allow to keep an exterior shipping package.  If the box has a 
shipping label it has to be emptied and placed in plastic tubs.


Debbie M. Boyd HT (ASCP) | Chief Histologist  | Southside Regional Medical 
Center | 200 Medical Park Blvd.  |  Petersburg, Va.  23805 | PH 804-765-5025 | 
FAX 804-765-6058


From: Paula Pierce [cont...@excaliburpathology.com]
Sent: Wednesday, June 03, 2015 11:42 AM
To: Goins, Tresa; Histonet
Subject: [EXTERNAL] Re: [Histonet] plants in the lab

Ugh. TOO MANY REGULATIONS!
What about plants and flowers taken to patient's rooms as get well wishes!?!
Soil on shoes? Incoming air every time the front doors open an infinite number 
of times a day?
Boxes supplies come in? Have you ever seen inside a semi truck trailer? The 
multiple holding docks boxes sit on awaiting transport?
We cannot live in a bubble.

 Paula Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue 
Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX 
www.excaliburpathology.com
  From: Goins, Tresa tgo...@mt.gov
 To: Tony Henwood (SCHN) tony.henw...@health.nsw.gov.au; Michelle Lamphere 
michelle.lamph...@childrens.com; 'histonet@lists.utsouthwestern.edu' 
histonet@lists.utsouthwestern.edu
 Sent: Wednesday, June 3, 2015 9:18 AM
 Subject: Re: [Histonet] plants in the lab

Patients do not have to go to the fungal spores, the spores will go to the 
patient.
Depending on spore size, the spores may stay airborne for months - the spores 
sediment to a surface in still air.
A condition not likely to occur in a hospital environment - they scurry around 
until finding a lung or mucous membrane to adhere to.
It doesn't take long for a single miss-handled Aspergillus culture plate to 
contaminate an entire multi-story research lab.



-Original Message-
From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au]
Sent: Tuesday, June 02, 2015 3:20 PM
To: Michelle Lamphere; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] plants in the lab

Hi Michelle,
Why would patients be in a histo lab anyway?


From: Michelle Lamphere [michelle.lamph...@childrens.com]
Sent: Sunday, 31 May 2015 10:36 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] plants in the lab

Our hospital Safety and Infection Control departments have policies in place 
prohibiting any potted plants from being in the hospital, anywhere.  We can 
have them if they are only in water, but the soil presents an infection control 
issue for patients because of potential mildew, mold, spores, etc.


Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
O: 214.456.2318 | Fax: 214.456.0779
E: michelle.lamph...@childrens.com
1935 Medical District Drive | B1.06  | Dallas, Texas  75235



Message: 2
Date: Fri, 29 May 2015 14:23:00 -0400
From: Blazek, Linda lbla...@digestivespecialists.com
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: [Histonet] plants in the lab
Message-ID:

5a2bd13465e061429d6455c8d6b40e391742126...@ibmb7exchange.digestivespecialists.com

Content-Type: text/plain; charset=us-ascii

Happy Friday all!

Does anyone have documentation of the benefit of having plants in the lab?  I 
know this was discusses quite a while ago but I can't find references for it.  
Any help would be appreciated.

Thanks,
Linda


Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with 
it contains information that is confidential and privileged. This information 
is intended only for the use of the
individual(s) and entity(ies) to whom it is addressed. If you are the intended 
recipient, further disclosures are prohibited without proper authorization. If 
you are not the intended recipient, any disclosure, copying, printing, or use 
of this information is strictly prohibited and possibly a violation of federal 
or state law and regulations. If you have received this information in error, 
please notify Children's Medical Center Dallas immediately via e-mail at 
priv...@childrens.com. Children's Medical Center Dallas and its affiliates 
hereby claim all applicable privileges related to this information

Re: [Histonet] [EXTERNAL] Re: plants in the lab

[Boyd, Debbie M]

There is a plethora of Joint Commission regulations concerning the below 
mentioned points of entry for microbacterial spores. If you are Joint 
Commissioned inspected, plants are not allowed in the lab.  We are JC inspected 
and are not even allow to keep an exterior shipping package.  If the box has a 
shipping label it has to be emptied and placed in plastic tubs.
 

Debbie M. Boyd HT (ASCP) | Chief Histologist  | Southside Regional Medical 
Center | 200 Medical Park Blvd.  |  Petersburg, Va.  23805 | PH 804-765-5025 | 
FAX 804-765-6058


From: Paula Pierce [cont...@excaliburpathology.com]
Sent: Wednesday, June 03, 2015 11:42 AM
To: Goins, Tresa; Histonet
Subject: [EXTERNAL] Re: [Histonet] plants in the lab

Ugh. TOO MANY REGULATIONS!
What about plants and flowers taken to patient's rooms as get well wishes!?!
Soil on shoes? Incoming air every time the front doors open an infinite number 
of times a day?
Boxes supplies come in? Have you ever seen inside a semi truck trailer? The 
multiple holding docks boxes sit on awaiting transport?
We cannot live in a bubble.

 Paula Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue 
Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX 
www.excaliburpathology.com
  From: Goins, Tresa tgo...@mt.gov
 To: Tony Henwood (SCHN) tony.henw...@health.nsw.gov.au; Michelle Lamphere 
michelle.lamph...@childrens.com; 'histonet@lists.utsouthwestern.edu' 
histonet@lists.utsouthwestern.edu
 Sent: Wednesday, June 3, 2015 9:18 AM
 Subject: Re: [Histonet] plants in the lab

Patients do not have to go to the fungal spores, the spores will go to the 
patient.
Depending on spore size, the spores may stay airborne for months - the spores 
sediment to a surface in still air.
A condition not likely to occur in a hospital environment - they scurry around 
until finding a lung or mucous membrane to adhere to.
It doesn't take long for a single miss-handled Aspergillus culture plate to 
contaminate an entire multi-story research lab.



-Original Message-
From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au]
Sent: Tuesday, June 02, 2015 3:20 PM
To: Michelle Lamphere; 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] plants in the lab

Hi Michelle,
Why would patients be in a histo lab anyway?


From: Michelle Lamphere [michelle.lamph...@childrens.com]
Sent: Sunday, 31 May 2015 10:36 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] plants in the lab

Our hospital Safety and Infection Control departments have policies in place 
prohibiting any potted plants from being in the hospital, anywhere.  We can 
have them if they are only in water, but the soil presents an infection control 
issue for patients because of potential mildew, mold, spores, etc.


Michelle Lamphere
Senior Tech, Histology
Anatomic Pathology
O: 214.456.2318 | Fax: 214.456.0779
E: michelle.lamph...@childrens.com
1935 Medical District Drive | B1.06  | Dallas, Texas  75235



Message: 2
Date: Fri, 29 May 2015 14:23:00 -0400
From: Blazek, Linda lbla...@digestivespecialists.com
To: histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Subject: [Histonet] plants in the lab
Message-ID:

5a2bd13465e061429d6455c8d6b40e391742126...@ibmb7exchange.digestivespecialists.com

Content-Type: text/plain; charset=us-ascii

Happy Friday all!

Does anyone have documentation of the benefit of having plants in the lab?  I 
know this was discusses quite a while ago but I can't find references for it.  
Any help would be appreciated.

Thanks,
Linda


Please consider the environment before printing this e-mail

This e-mail, facsimile, or letter and any files or attachments transmitted with 
it contains information that is confidential and privileged. This information 
is intended only for the use of the
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recipient, further disclosures are prohibited without proper authorization. If 
you are not the intended recipient, any disclosure, copying, printing, or use 
of this information is strictly prohibited and possibly a violation of federal 
or state law and regulations. If you have received this information in error, 
please notify Children's Medical Center Dallas immediately via e-mail at 
priv...@childrens.com. Children's Medical Center Dallas and its affiliates 
hereby claim all applicable privileges related to this information.


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[Histonet] external alarm for Tissue-Tek VIP E300

[Juliana Montero]

I am looking to get an external alarm for our tissue processor. Any suggestions?



Juliana Montero, HTL

Lead Histotechnologist



USMD Pathology Laboratory

900 Airport FWY

Ste. 132

Hurst, TX 76054



817-576-0590 Office

817-576-0591 Fax

432-238-8981 Cell



juliana.mont...@usmd.commailto:juliana.mont...@usmd.com







[USMD]


The information contained in this e-mail message is intended exclusively for 
the recipient(s) named above. This communication may contain information that 
is confidential, proprietary or otherwise legally exempt from disclosure. If 
you are not the named addressee, you are not authorized to read, print, retain, 
copy or disseminate this message or any part of it.If you have received this 
message in error, please notify the sender immediately by e-mail and delete all 
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recipient does not waive a privileged relationship (if applicable) between the 
sender and the recipient.

USMDhttp://www.usmdinc.com/

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RE: [Histonet] external alarm for Tissue-Tek VIP E300

[Douglas Porter]

We have ours tied into our security system so that when the alarm goes off
we get a call from the security company.  Works quite well!

Douglas A. Porter, HT (ASCP) 
Grossing Technician 
IT Coordinator
Cancer Registrar 

CAP-Lab, PLC 
2508 South Cedar Street 
Lansing, MI 48910-3138 

517-372-5520 (phone) 
517-372-5540 (fax) 

doug.por...@caplab.org 

www.caplab.org  
 
 
The information contained in this message may be privileged and/or
confidential and protected from disclosure. If the reader of this message is
not the intended recipient, you are hereby notified that any dissemination,
distribution, copying, forwarding or capture of this communication is
strictly prohibited. If you have received this communication in error,
please notify me immediately by return e-mail and delete this and all
copies. Thank-you.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Juliana
Montero
Sent: Monday, November 18, 2013 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] external alarm for Tissue-Tek VIP E300

I am looking to get an external alarm for our tissue processor. Any
suggestions?



Juliana Montero, HTL

Lead Histotechnologist



USMD Pathology Laboratory

900 Airport FWY

Ste. 132

Hurst, TX 76054



817-576-0590 Office

817-576-0591 Fax

432-238-8981 Cell



juliana.mont...@usmd.commailto:juliana.mont...@usmd.com







[USMD]


The information contained in this e-mail message is intended exclusively for
the recipient(s) named above. This communication may contain information
that is confidential, proprietary or otherwise legally exempt from
disclosure. If you are not the named addressee, you are not authorized to
read, print, retain, copy or disseminate this message or any part of it.If
you have received this message in error, please notify the sender
immediately by e-mail and delete all copies of the message. Review by any
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relationship (if applicable) between the sender and the recipient.

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RE: [Histonet] external alarm for Tissue-Tek VIP E300

[Terri Brown]

We use Amega Scientific Corporation and get great service.

Terri H. Brown,, HT (ASCP)
Pathology Laboratory Manager
Northside Hospital  Atlanta
terri.br...@northside.com

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Juliana
Montero
Sent: Monday, November 18, 2013 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] external alarm for Tissue-Tek VIP E300

I am looking to get an external alarm for our tissue processor. Any
suggestions?



Juliana Montero, HTL

Lead Histotechnologist



USMD Pathology Laboratory

900 Airport FWY

Ste. 132

Hurst, TX 76054



817-576-0590 Office

817-576-0591 Fax

432-238-8981 Cell



juliana.mont...@usmd.commailto:juliana.mont...@usmd.com







[USMD]


The information contained in this e-mail message is intended exclusively
for the recipient(s) named above. This communication may contain
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the sender immediately by e-mail and delete all copies of the message.
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RE: [Histonet] external alarm for Tissue-Tek VIP E300

[joelle weaver]

Medical Equipment Source LLC
i...@medequiosourc.com 724-625-2235
 
Had some good solutions for this type of issue 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
 From: juliana.mont...@usmd.com
 To: histonet@lists.utsouthwestern.edu
 Date: Mon, 18 Nov 2013 16:03:48 +
 Subject: [Histonet] external alarm for Tissue-Tek VIP E300
 
 I am looking to get an external alarm for our tissue processor. Any 
 suggestions?
 
 
 
 Juliana Montero, HTL
 
 Lead Histotechnologist
 
 
 
 USMD Pathology Laboratory
 
 900 Airport FWY
 
 Ste. 132
 
 Hurst, TX 76054
 
 
 
 817-576-0590 Office
 
 817-576-0591 Fax
 
 432-238-8981 Cell
 
 
 
 juliana.mont...@usmd.commailto:juliana.mont...@usmd.com
 
 
 
 
 
 
 
 [USMD]
 
 
 The information contained in this e-mail message is intended exclusively for 
 the recipient(s) named above. This communication may contain information that 
 is confidential, proprietary or otherwise legally exempt from disclosure. If 
 you are not the named addressee, you are not authorized to read, print, 
 retain, copy or disseminate this message or any part of it.If you have 
 received this message in error, please notify the sender immediately by 
 e-mail and delete all copies of the message. Review by any individual other 
 than the intended recipient does not waive a privileged relationship (if 
 applicable) between the sender and the recipient.
 
 USMDhttp://www.usmdinc.com/
 
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[Histonet] External UV for a Leica 1850?

[Paula Sicurello]

Hello Fellow Netters,

Has anyone tried using some type of external UV source to decontaminate a
Leica 1850 cryostat?  I found out that it is not possible to retro fit the
1850 for UV.  I would like to be able to avoid having to defrost, breakdown
and bleach the cryostat everytime a suspected infectious tissue is cut in
it.

Suggestions kindly welcomed.

Thanks!

Paula

-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091

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Re: [Histonet] External UV for a Leica 1850?

[Lee Peggy Wenk]

If you are a CAP accredited lab, CAP says that the cryostat must be 
defrosted and disinfectant decontaminated at regular intervals with a TB 
disinfectant.


- - -
ANP.23410 Cryostat Decontamination Phase II
There is a documented procedure for the routine decontamination of the 
cryostat at defined intervals, and decontamination records are evident.
NOTE: The cryostat must be defrosted and decontaminated by wiping all 
exposed surfaces with tuberculocidal disinfectant. The cryostat should be at 
room temperature during decontamination unless otherwise specified by the 
manufacturer. This should be done at an interval appropriate for the 
institution; this must be weekly for instruments used daily. Trimmings and 
sections of tissue that accumulate inside the cryostat must be removed 
during decontamination. Although not a requirement, steel mesh gloves should 
be worn when changing knife blades.

- - - -

Even if you can use a UV light, ALL debris/contaminants must be removed from 
the cryostat chamber BEFORE using the UV light. The germicidal effect of 
radiation is only good on the areas that the UV light can hit directly. So 
any little corners, or areas under metal plates, or areas under the 
OCT/tissue shavings will not be directly illuminated by the UV light, and 
thus will not be disinfected.


There are also different types of UV lamps. I have heard that low efficiency 
UV lamps need a long period of time of being turned on to disinfect, and 
that this long exposure in a small area of the chamber of the cryostat can 
produce a high level of ozone in the chamber, so there could be an ozone 
exposure level to the tech using the cryostat.


So, UV light can be used in CONJUNCTION with wiping out, chemical 
disinfecting, and defrosting. But I don't believe it can be used IN PLACE of 
wiping out, chemical disinfecting, and defrosting.


Peggy A. Wenk, HTL(ASCP)SLS

-Original Message- 
From: Paula Sicurello

Sent: Thursday, October 10, 2013 2:36 PM
To: HistoNet
Subject: [Histonet] External UV for a Leica 1850?

Hello Fellow Netters,

Has anyone tried using some type of external UV source to decontaminate a
Leica 1850 cryostat?  I found out that it is not possible to retro fit the
1850 for UV.  I would like to be able to avoid having to defrost, breakdown
and bleach the cryostat everytime a suspected infectious tissue is cut in
it.

Suggestions kindly welcomed.

Thanks!

Paula

--
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091

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Re: [Histonet] External UV for a Leica 1850?

[Traczyk7]

Paula,
A UV insert in available from Hacker Instruments. (1-800-4-HACKER) It was  
not designed specifically for an 1850, but I don't see why it would not work 
out  for you. Give Jim Mullen a call, he should be able to give you the 
device  dimensions and power specifications.
Have a great day.
Dorothy
 
Dorothy Traczyk BS, HT(ASCP)
Histology Technician
Monmouth Medical Center
Long Branch, NJ 07740
 
 
In a message dated 10/10/2013 2:36:42 P.M. Eastern Daylight Time,  
pat...@gmail.com writes:

Hello  Fellow Netters,

Has anyone tried using some type of external UV source  to decontaminate a
Leica 1850 cryostat?  I found out that it is not  possible to retro fit the
1850 for UV.  I would like to be able to  avoid having to defrost, breakdown
and bleach the cryostat everytime a  suspected infectious tissue is cut in
it.

Suggestions kindly  welcomed.

Thanks!

Paula

-- 
Paula Sicurello, HTL  (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke  University Health System
Rm.#251M, Duke South, Green Zone
Durham, North  Carolina 27710
P:  919.684.2091

HIPAA Privacy Notification:  This message and any accompanying documents are
covered by the Electronic  Communications Privacy Act, 18 U.S.C. 2510-2521,
and contain information  intended for the specific individual (s) only. This
information is  confidential. If you are not the intended recipient or an
agent responsible  for delivering it to the intended recipient, you are
hereby notified that  you have received this document in error and that any
review,  dissemination, copying or the taking of any action based on the
contents of  this information is strictly prohibited . If you have received
this  communication in error, please notify us immediately by e-mail, and
delete  the original  message.
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