Re: [ccp4bb] Rigaku CrystalTrak Web not working

I posted this offline to Pavel, but maybe there are more people out there who might find it useful? Hi Pavel, We have built and use See3, which is a replacement for CTweb that doesn't use flash. It still uses the CM (Crystal) database. If you are interested you can check it out (it would be

Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

Hi all, Interesting discussion, and personally, I think Rasmus has hit the nail on the head. Here is a challenge to the CCP4bb - can we make a list 10 female developers of crystallographic software who would be appropriate invitees as instructors to this course? (I can only think of 4)

Re: [ccp4bb] Protein concentration for the initial crystallisation trials

Hi, Another way of estimating a starting protein concentration is to watch your concentration process – if your protein is in a spin concentrator (with an appropriate membrane cutoff size say ~ [MW protein]/3) and is losing volume really quickly then keep going. As soon as the concentration

Re: [ccp4bb] how to get protein crystal

Hi, To add to this thread, there are a few more easy things to try – Try doing matrix microseeding and try doing limited proteolysis. Even though the following link describes how we do this in our laboratory, (the C3 Facility in Melbourne) the pages give a quick overview to both matrix

Re: [ccp4bb] Crystal screen for DNA binding proteins

And if you log in to C6 (c6.csiro.au - one can use the guest account: click on "Or click here to try guest account") - navigate to "screens list"=>"commercial screens" => KERAfast => and if you click on the screen then it will come up with a description of what is in every well, and (most) of

Re: [ccp4bb] [OT] Structure-related pun needed urgently

"I think my behaviour is in the allowed region, but others think I'm a right out liar" Janet Newman Principal Scientist / Director, Collaborative Crystallisation Centre (C3) CSIRO Material Science and Engineering 343 Royal Parade Parkville. VIC. 3052 Australia Tel +613 9662 7326 Email

Re: [ccp4bb] Importance of temperature during initial crystallization screening

Interesting topic, Certainly the two papers suggested by Georg are relevant, and I fully agree with the comments from Daniel that it is hard to predict the behaviour of any given protein from a statistical analysis of proteins in general. I find it interesting that even with the use of

Re: [ccp4bb] challenges in structural biology

There are a bunch of people doing this – in the small molecule world. And a lot of work has been done on some very robust protein systems too. Can you guess which ones? The real issue (at the moment) is that all the pre-work needed to predict if or how a protein might crystallise takes more

Re: [ccp4bb] Re-using 96-well crystallization plates

Hi Nemanja, I have tried doing this, and it has never really worked for me, even with careful rinsing with MilliQ water after washing, I could never get well-shaped drops on a recycled plate. They are also a real pain to wash out, and it’s hard to get the last traces of protein out of the

Re: [ccp4bb] Interesting pattern on a crystallization drop

I'm guessing quite big drops on a somewhat hydrophobic surface? Also guessing that the protein foams quite a bit, and that there were 6 (or four) more or less equal size bubbles that took up almost the whole drop when it was set up. Bubbles tend to pack quite efficiently