Re: [ccp4bb] Rigaku CrystalTrak Web not working

I posted this offline to Pavel, but maybe there are more people out there who might find it useful? Hi Pavel, We have built and use See3, which is a replacement for CTweb that doesn't use flash. It still uses the CM (Crystal) database. If you are interested you can check it out (it would be a

Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

Hi all, Interesting discussion, and personally, I think Rasmus has hit the nail on the head. Here is a challenge to the CCP4bb - can we make a list 10 female developers of crystallographic software who would be appropriate invitees as instructors to this course? (I can only think of 4) Cheers,

Re: [ccp4bb] Protein concentration for the initial crystallisation trials

Hi, Another way of estimating a starting protein concentration is to watch your concentration process – if your protein is in a spin concentrator (with an appropriate membrane cutoff size say ~ [MW protein]/3) and is losing volume really quickly then keep going. As soon as the concentration st

Re: [ccp4bb] how to get protein crystal

Hi, To add to this thread, there are a few more easy things to try – Try doing matrix microseeding and try doing limited proteolysis. Even though the following link describes how we do this in our laboratory, (the C3 Facility in Melbourne) the pages give a quick overview to both matrix microsee

Re: [ccp4bb] Crystal screen for DNA binding proteins

And if you log in to C6 (c6.csiro.au - one can use the guest account: click on "Or click here to try guest account") - navigate to "screens list"=>"commercial screens" => KERAfast => and if you click on the screen then it will come up with a description of what is in every well, and (most) of t

Re: [ccp4bb] [OT] Structure-related pun needed urgently

"I think my behaviour is in the allowed region, but others think I'm a right out liar" Janet Newman Principal Scientist / Director, Collaborative Crystallisation Centre (C3) CSIRO Material Science and Engineering 343 Royal Parade Parkville. VIC. 3052 Australia Tel +613 9662 7326 Email janet.new.

Re: [ccp4bb] Importance of temperature during initial crystallization screening

Interesting topic, Certainly the two papers suggested by Georg are relevant, and I fully agree with the comments from Daniel that it is hard to predict the behaviour of any given protein from a statistical analysis of proteins in general. I find it interesting that even with the use of incubato

Re: [ccp4bb] challenges in structural biology

There are a bunch of people doing this – in the small molecule world. And a lot of work has been done on some very robust protein systems too. Can you guess which ones? The real issue (at the moment) is that all the pre-work needed to predict if or how a protein might crystallise takes more wor

Re: [ccp4bb] challenges in structural biology

Throwing my hat in with Artem's here - Not only is there limited funding for (biological) crystallisation, there is perhaps even less interest. Some of the reason is that what we do now works much of the time, so why actually think about things when another screen (or another construct) might d

Re: [ccp4bb] Re-using 96-well crystallization plates

Hi Nemanja, I have tried doing this, and it has never really worked for me, even with careful rinsing with MilliQ water after washing, I could never get well-shaped drops on a recycled plate. They are also a real pain to wash out, and it’s hard to get the last traces of protein out of the subwe

Re: [ccp4bb] Interesting pattern on a crystallization drop

I'm guessing quite big drops on a somewhat hydrophobic surface? Also guessing that the protein foams quite a bit, and that there were 6 (or four) more or less equal size bubbles that took up almost the whole drop when it was set up. Bubbles tend to pack quite efficiently (https://physicsworld.c