I would suggest the same
Best
__
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São
Dear John
Ethanol would be my first guess,
Best wishes
__
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
It reminds me of that movie, the wishmaster, a thrash one. “Be careful about
what you desire”
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São
Hi,
It seems to my eyes that you have multiple nucleation events happening
simultaneously. I would recommend you to try crystallization under 10 or 4 ºC
Best of luck
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de
It seems to me that your drop has dried. By the pic, I would not say these are
crystals
Best
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São
I tried it yesterday and I was really shocked by how fast it is. When I was preparing to submit my second job, the first one was already finished, which made me think that I was definitely doing something wrong. Probably I was...Best wishes Rafael Marques On 13 May 2024 09:53, Harry Powell
3.2 A
structure. How such a nice map could have been calculated with such poor
parameters? I could only think of anisotropy.
Best
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciênc
concentration?
Best wishes
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A sorte acompanha uma ment
Hi all. Just wanted to add a bit of doubt to the topic. Is it possible that
they are salt crystals? I don’t know, but some spots are too intense and far
from each other to me. But maybe I just need better glasses.
Best wishes
Rafael Marques da Silva
PhD Student – Structural Biology
University
it using an in-house
system you should consider a synchrotron light source.
Best wishes
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
not diffract, I think it is
something you should try with the remaining ones. Alternatively, if you have a
source of UV light coupled to a camera, they should “light up”. I wish you the
best of luck.
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física
Hi Sam.
If you still have any of your crystals or any protein solution left in the well
you harvested your crystals, I would run a MS/MS with them. Next step would be
to run AF with your known chain A and your best Mass Spec hit (s), and use the
resulting model for MR.
Good luck
Rafael
resin (or maybe do not add both). I saw
that someone did not have EDTA and used b-mercap. instead of DTT. May I have
your comments if you guys already faced a similar situation?
Best wishes
__
Rafael Marques da Silva
PhD Student – Structural
scattering, could you please send me? I am preparing a
presentation and a single google search has been really misleading.
Best wishes
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências
The same happened to me. I had to subscribe again cause I was not receiving
ccp4 mails anymore.
Best
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal
will take a look
on the sites and in chimera.
Best wishes
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
would like to know “how much” red it is.
Best wishes
Rafael Marques da Silva
PhD Student – Structural Biology
University of Leicester
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
Problematic is low multiplicity, not high, I’d say...
Best
Rafael Marques da Silva
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A sorte acompanha uma mente bem tre
I am not a proper chemist, but I remember that during my college classes my
professor emphasized that ^x is a measured value and not really related to
stoichiometry. If someone has something to add…
Best
Rafael Marques da Silva
Mestre em Física Biomolecular
Universidade de São Paulo
Bacharel
If Paul had said that there is also a Windows version I would be sure that it
was April fools’ joke. I can’t wait to use it
Best
Rafael Marques da Silva
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55
), the following documents
for registration:
– A letter presenting your interest;
– A short CV, presenting academic background, publications, and information
that proves your scientific experience;
- Letter of recommendation.
Best Regards
Rafael Marques da Silva
Mestrando em Física Biomolecular
Universidade
oligomers in
solution. You can try native gel to find this out but firstly I would use
another SEC column, a bigger one. Superdex 200 10/300 is an analytical column
and should not be used for purification itself (although I have done this many
times). I would try maybe S200 16/60.
Best
Rafael
. Are your proteins monomers in
solution? If you are not sure, try a bigger SEC column.
Regards
Rafael Marques da Silva
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A
couldn’t get another space group till now. And yes, there is a lot of solvent
(~79%) and I believe that this is behind with this protein crystal don’t
diffract beyond the necessary.
Thank you very much again, guys
Best regards
Rafael Marques da Silva
Mestrando em Física Biomolecular
can find files for doing SAD,
MAD, MR, SIRAS and so on?
Many thanks in advance
__
Rafael Marques da Silva
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55
Thank you for pointing me this out. I don’t know why I said BL21...maybe in the
rush to say to use Rosetta for protein expression. We use Dh5a for plasmid
replication (although I have already used BL21 for plasmid purification and it
worked nicely). My bad.
Rafael Marques da Silva
Mestrando
__
Rafael Marques da Silva
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A sorte acompanha uma mente bem tre
share it with me.
Kind Regards
__
Rafael Marques da Silva
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A sorte acom
group we have got better diffracting crystals using
25mg/mL)
Good luck
Rafael Marques da Silva
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A sorte acompanha uma ment
Lol
__
Rafael Marques da Silva
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências Biológicas
Universidade Federal de São Carlos
phone: +55 16 99766-0021
"A sorte acompanha uma mente bem tre
a Thunders, a sixteen years old teenager activist , is better
known than the experts in the field. If we want to be believed we must take
back our place. To hind is not an option.
Regards
Rafael Marques da Silva
Mestrando em Física Biomolecular
Universidade de São Paulo
Bacharel em Ciências
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