Re: [ccp4bb] Plasmids for Fluorescent proteins

Hi Prerna, Addgene is the first & best place to look for such commonly desired plasmids or proteins! Best, Reza Sent from my iPhone > On Jul 13, 2024, at 11:53 PM, P G wrote: > >  > Hello everyone, > We are looking for some fluorescent proteins for undergraduate research (GFP, > BFP, DsRed

Re: [ccp4bb] Multi-lattice crystal data processing strategy for structure solution

Hi Devbrat,Have you tried indexing using XDS multi-lattice processing? It is a very useful indexing process for multi-lattice and/or 2nd lattice interference with the major one. Best,Rezaul Karim, Ph.D.Structural Biology ScientistNurix Therapeutics Inc.Sent from my iPhoneOn Nov 15, 2023, at 6:22

Re: [ccp4bb] High Temperature Factors with TLS

If  the correct atoms are in place, resetting the b factors of those atoms/residues to a constant e.g. 5.00 and refining in phenix with individual B-factors ON should take care of these.Best,RezaSent from my iPhoneOn Aug 2, 2023, at 7:42 AM, Thomas, Leonard M. wrote: Hello All, A general

Re: [ccp4bb] missing covalent bond between asparagine and N-

yset)Refine that pdb in Phenix without hydrogen.Then check it in coot & pymol. Even coot shows dotted lines, pymol will show a solid bond!Hope it helps! Best,Reza Md Rezaul Karim, Ph.D. Postdoctoral Research Fellow Department of Drug Discovery H. Lee Moffitt Cance

Re: [ccp4bb] To solve the problem of an extremely asymmetric

of these could affect separation efficiency and/or peak characteristics. Regards,Reza “The rule for web content is to keep it short. The rule for email content is to keep it ultra-short.” — Jakob Nielsen, ‘king of usability’ ________ Md Rezaul Karim, Ph.D. Postdoctoral R

Re: [ccp4bb] Best protocols to advance a low resolution twin

data (~95).  Thanks,Reza Md Rezaul Karim PhD candidate PhD Program in Integrated Biomedical Sciences Dept. of Molecular Medicine, Morsani College of Medicine, USF,Tampa Schonbrunn lab, Moffitt Cancer Center, Tampa Email: rez...@health.usf.edu, md.ka...@moffitt.org Phone: (813) 745 4673 ext. 5462

Re: [ccp4bb] Best protocols to advance a low resolution twin

. Thanks,Reza Md Rezaul Karim PhD candidate PhD Program in Integrated Biomedical Sciences Dept. of Molecular Medicine, Morsani College of Medicine, USF,Tampa Schonbrunn lab, Moffitt Cancer Center, Tampa Email: rez...@health.usf.edu, md.ka...@moffitt.org Phone: (813) 745 4673 ext. 5462 On Mon

Re: [ccp4bb] Best protocols to advance a low resolution twin

ein band on SDS at right MW. Data from 20+ crystals from various condition & additive screens came out all the same.Resorted to changing the construct to make variable N/C-term for future. Thanks,Reza Md Rezaul Karim Postdoctoral Research FellowH. Lee Moffitt Cancer Center and R

Re: [ccp4bb] Vote for cryoEM

. don't believe in climate change or it's a hoax) but don't listen to scientists- then twitter could be blamed, but science fails. Thanks,Reza Md. Rezaul Karim (Reza) Ph.D. candidate, PhD Program in Integrated Biomedical SciencesDepartment of Molecular MedicineMorsani College of MedicineUniversity

Re: [ccp4bb] aimless outputs mean intensities for friedel pairs

some clue whether this is the case. Another alternative is xdsconv. Any advantage of using pointless and aimless instead of xdsconv for your purpose? Thanks,Reza Md. Rezaul Karim (Reza) Ph.D. candidate, PhD Program in Integrated Biomedical SciencesDepartment of Molecular MedicineMorsa

Re: [ccp4bb] ITC Stoichiometry

Hi Angshu,Very interesting experiment. Is the B is in hexameric form in solution by itself or it requires A for hexamer formation? Thanks,Reza Md Rezaul Karim PhD candidate PhD Program in Integrated Biomedical Sciences Dept. of Molecular Medicine, Morsani College of Medicine, USF,Tampa Schonbrunn

Re: [ccp4bb] determining the point group and the space group

On Thu, Apr 19, 2018 at 11:32 AM, Rezaul Karim<reza...@yahoo.com> wrote: I think Graeme's point is right. This is the work expert crystallographer. By the way, DIALS could be used for XFEL data, as the program page & recent publication indicates. NB: I have no experien