29 7077
From: CCP4 bulletin board on behalf of Paul Emsley
Sent: 27 March 2020 21:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Covalent Cysteine Aduct
On 27/03/2020 21:06, Cowan, Richard H. (Dr.) wrote:
although [BME] seems unlikely, since the crystallized protein is a Fab.
I don'
niversity of LeicesterLancaster
RoadLeicester, LE1 9HN, U.K. Phone +44 (0) 116 229 7077
From: CCP4 bulletin board on behalf of Paul Emsley
Sent: 27 March 2020 21:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Covalent Cysteine Aduct
On 27/03/2020 21:06, Cowan, Richard H. (Dr.) wrote:
although
ailto:pems...@mrc-lmb.cam.ac.uk>>
Sent: 27 March 2020 21:33
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
mailto:CCP4BB@JISCMAIL.AC.UK>>
Subject: Re: [ccp4bb] Covalent Cysteine Aduct
On 27/03/2020 21:06, Cowan, Richard H. (Dr.) wrote:
although [BME] seems unlikely, sinc
5
>Department of Biochemistry
>University of Leicester
>Lancaster Road
>Leicester, LE1 9HN, U.K.
>
>Phone +44 (0) 116 229 7077
>
>
>
>
>
>
>________
>From: Mark J van Raaij
>Sent: 27 March 2020 21:46
>To: Cowan, Ri
>
> Sent: 27 March 2020 21:33
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Covalent Cysteine Aduct
>
>
>
>> On 27/03/2020 21:06, Cowan, Richard H. (Dr.) wrote:
>>
>>
>> although [BME] seems unlikely, since the crystallized
ilto:CCP4BB@JISCMAIL.AC.UK>>
on behalf of Paul Emsley
mailto:pems...@mrc-lmb.cam.ac.uk>>
Sent: 27 March 2020 21:33
To: CCP4BB@JISCMAIL.AC.UK<mailto:CCP4BB@JISCMAIL.AC.UK>
mailto:CCP4BB@JISCMAIL.AC.UK>>
Subject: Re: [ccp4bb] Covalent Cysteine Aduct
On 27/03/2020 21:06, Cowan, Ric
S-hydroxycysteine can cartainly show up under nonreducing storage or
crystallization conditions, as an artifact.
Cheers,
Roger Rowlett
On Fri, Mar 27, 2020, 5:45 PM Chris Fage wrote:
> Hi Richard,
>
> I recently observed the sulfenic acid derivative of a cysteine residue
> (S-hydroxy-Cys, liga
I see. Might there be some discrepancy between best practice and what
actually happened? ?
On 27/03/2020 21:38, Cowan, Richard H. (Dr.) wrote:
The Fab constructs have a c-terminal cysteines on both the heavy and
light chains, which should form a disulphide. Adding reducing agent to
the
Hi Richard,
I recently observed the sulfenic acid derivative of a cysteine residue
(S-hydroxy-Cys, ligand ID CSO) in one of my high-resolution maps. Could it
be possibly be this, H-bonded to a nearby water molecule?
Best wishes,
Chris
On Fri, Mar 27, 2020 at 21:33 Paul Emsley wrote:
>
> On 27
2020 21:33
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Covalent Cysteine Aduct
On 27/03/2020 21:06, Cowan, Richard H. (Dr.) wrote:
although [BME] seems unlikely, since the crystallized protein is a Fab.
I don't follow.
To unsubscribe from
On 27/03/2020 21:06, Cowan, Richard H. (Dr.) wrote:
although [BME] seems unlikely, since the crystallized protein is a Fab.
I don't follow.
To unsubscribe from the CCP4BB list, click the following link:
https://www
owan, Richard H. (Dr.)
Subject: re: [ccp4bb] Covalent Cysteine Aduct
Also, are you sure of protein sequence. Density looked like a long side chain
(Lys or Arg) so always worth considering.
Best,
Andrew
--
Andrew M. Gulick,
Ph.D.<https://eur03.safelinks.protection.outlook.c
Road
Leicester, LE1 9HN, U.K.
Phone +44 (0) 116 229 7077
From: Petri Kursula
Sent: 27 March 2020 21:13
To: Cowan, Richard H. (Dr.)
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4bb] Covalent Cysteine Aduct
Looks like DTT adduct to me; but then again, if you did not have i
Dr Richard Cowan
> Research Associate
> <>
> HWLSB 1/05
> Department of Biochemistry
> University of Leicester
> Lancaster Road
> Leicester, LE1 <> 9HN <>, U.K.
>
> Phone +44 (0) 116 229 7077
>
> From: robbie_joos...@hotmail.com
> Sent: 27 March
otmail.com
Sent: 27 March 2020 20:57
To: Cowan, Richard H. (Dr.)
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Covalent Cysteine Aduct
Are you absolutely sure about the conditions? The blob next to the sulfur looks
pretty fat so I would guess BME.
Cheers,
Robbie
On 27 Mar 2020 21:32, "C
Are you absolutely sure about the conditions? The blob next to the sulfur looks pretty fat so I would guess BME.Cheers,RobbieOn 27 Mar 2020 21:32, "Cowan, Richard H. (Dr.)" wrote:
Hi All,
During the current enforced shutdown, I've been going back over some older data and spotted this in one o
It looks like the density suggests an S-S bond. Perhaps try CME?
https://www.rcsb.org/ligand/CME
John J. Tanner
Professor of Biochemistry and Chemistry
Associate Chair of Biochemistry
University of Missouri
117 Schweitzer Hall
503 S. College Ave.
Columbia, MO 65211
Phone: 573-884-1280
Fax: 573-
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