of Sam Arnosti
[meisam.nosr...@gmail.com]
Sent: Monday, November 07, 2011 7:19 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystalization in low PH
Hi everyone
I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
I want to crystallize it in the low PH and compare
Tendamistat (1OK0) was crystallized at pH 1.3 and diffracted to 0.93A.
George
On Mon, Nov 07, 2011 at 05:19:29AM +, Sam Arnosti wrote:
Hi everyone
I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
I want to crystallize it in the low PH and compare the differences
I'm not convinced that you need a conventional buffer at pH 2 or 3. At pH 2,
the hydrogen ion concentration is 10 mM. If you want to use something else,
the second pKa for sulfuric acid is around 2. The first pKa for phosphoric
acid is slightly higher than 2. Lactic acid has a pKa close to
I have crystallized in PEG with citrate at pH 3. If you want to go lower
I would suggest maleate:
effective pH range pKa 25°Cbuffer
1.2-2.6 1.97 maleate (pK1)
2.2-6.53.13 citrate (pK1)
Enrico.
On Mon, 07
I remembered that people had crystallize a series of
streptavidin-2-iminobiotin structures at a low pH. If it might help, check
the following PDBIDs:
2RTD
2RTE
2RTI
2RTK
2RTL
Hi everyone
I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
I want to crystallize it in the
On Mon, 2011-11-07 at 05:19 +, Sam Arnosti wrote:
Hi everyone
I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
I want to crystallize it in the low PH and compare the differences between
the crystals in regular PH and low PH.
I was wondering how people set up
Glutaraldehyde works best at low pH
On Mon, Nov 7, 2011 at 8:40 AM, Ed Pozharski epozh...@umaryland.edu wrote:
On Mon, 2011-11-07 at 05:19 +, Sam Arnosti wrote:
Hi everyone
I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
I want to crystallize it in the low PH and
Hi everyone
I have a protein that is extraordinarily stable at PH=3.0 or even 2.0.
I want to crystallize it in the low PH and compare the differences between the
crystals in regular PH and low PH.
I was wondering how people set up the boxes in low PH, as usual buffers are
mostly less acidic.