Re: [ccp4bb] Femtosecond Electron Beam

2011-04-15 Thread Petr Leiman
: Re: [ccp4bb] Femtosecond Electron Beam Colin, We know that with a dose of 20-30 electrons per A^2, a lot of image processing, and insane amount of luck, one can reconstruct cryoEM images to 3 A resolution or better. A typical protein molecule is say 100 A in diameter, which is ~8000 A^2

[ccp4bb] Femtosecond Electron Beam

2011-04-14 Thread Jacob Keller
Dear Crystallographers, is there any reason why we are not considering using super-intense femtosecond electron bursts, instead of photons? Since the scattering of electrons is much more efficient, and because they can be focussed to solve the phase problem, it seems that it might be worthwhile

Re: [ccp4bb] Femtosecond Electron Beam

2011-04-14 Thread Colin Nave
the details. Regards Colin -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob Keller Sent: 14 April 2011 14:39 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Femtosecond Electron Beam Dear Crystallographers, is there any reason why we

Re: [ccp4bb] Femtosecond Electron Beam

2011-04-14 Thread Petr Leiman
: Thursday, April 14, 2011 16:50 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Femtosecond Electron Beam Jacob Very good question. People are considering this sort of thing. See for example http://www-spires.slac.stanford.edu/cgi-wrap/getdoc/slac-pub-12162.pdf Due to coulomb explosion one normally

Re: [ccp4bb] Femtosecond Electron Beam

2011-04-14 Thread Jrh
Dear Jacob, Ahmed Zewail's papers are worth consulting on this, although not protein/bio. See also the book by Zewail and Thomas, recently published, easily findable on amazon etc, as a handy overview. Best wishes, John Prof John R Helliwell DSc On 14 Apr 2011, at 14:38, Jacob Keller

Re: [ccp4bb] Femtosecond Electron Beam

2011-04-14 Thread Colin Nave
: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Petr Leiman Sent: 14 April 2011 16:23 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Femtosecond Electron Beam People are looking into how to fit the old retired MeV microscopes with pulsed electron guns (problem

Re: [ccp4bb] Femtosecond Electron Beam

2011-04-14 Thread Petr Leiman
] On Behalf Of Petr Leiman Sent: 14 April 2011 16:23 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Femtosecond Electron Beam People are looking into how to fit the old retired MeV microscopes with pulsed electron guns (problem is there are very few of those beasts left). If this works

Re: [ccp4bb] Femtosecond Electron Beam

2011-04-14 Thread Colin Nave
@JISCMAIL.AC.UK] On Behalf Of Petr Leiman Sent: 14 April 2011 21:07 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Femtosecond Electron Beam Dear Colin and all interested in the FEL development. Please look at the figures in the first link I mentioned. Jom Luiten et al. are able to record

Re: [ccp4bb] Femtosecond Electron Beam

2011-04-14 Thread Petr Leiman
Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Petr Leiman Sent: 14 April 2011 21:07 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Femtosecond Electron Beam Dear Colin and all interested in the FEL development. Please look at the figures in the first

Re: [ccp4bb] Femtosecond Electron Beam

2011-04-14 Thread Colin Nave
: [ccp4bb] Femtosecond Electron Beam Colin, We know that with a dose of 20-30 electrons per A^2, a lot of image processing, and insane amount of luck, one can reconstruct cryoEM images to 3 A resolution or better. A typical protein molecule is say 100 A in diameter, which is ~8000 A^2

Re: [ccp4bb] Femtosecond Electron Beam

2011-04-14 Thread Jacob Keller
-Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Petr Leiman Sent: 14 April 2011 22:59 To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Femtosecond Electron Beam Colin, We know that with a dose of 20-30 electrons per A^2, a lot of image processing