: Re: [ccp4bb] Femtosecond Electron Beam
Colin,
We know that with a dose of 20-30 electrons per A^2, a lot of image
processing, and insane amount of luck, one can reconstruct cryoEM
images to 3 A resolution or better. A typical protein molecule is
say
100 A in diameter, which is ~8000 A^2
Dear Crystallographers,
is there any reason why we are not considering using super-intense
femtosecond electron bursts, instead of photons? Since the scattering
of electrons is much more efficient, and because they can be focussed
to solve the phase problem, it seems that it might be worthwhile
the details.
Regards
Colin
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Jacob Keller
Sent: 14 April 2011 14:39
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Femtosecond Electron Beam
Dear Crystallographers,
is there any reason why we
: Thursday, April 14, 2011 16:50
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Femtosecond Electron Beam
Jacob
Very good question.
People are considering this sort of thing. See for example
http://www-spires.slac.stanford.edu/cgi-wrap/getdoc/slac-pub-12162.pdf
Due to coulomb explosion one normally
Dear Jacob,
Ahmed Zewail's papers are worth consulting on this, although not protein/bio.
See also the book by Zewail and Thomas, recently published, easily findable on
amazon etc, as a handy overview.
Best wishes,
John
Prof John R Helliwell DSc
On 14 Apr 2011, at 14:38, Jacob Keller
: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Petr Leiman
Sent: 14 April 2011 16:23
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Femtosecond Electron Beam
People are looking into how to fit the old retired MeV microscopes with
pulsed electron guns (problem
] On Behalf Of
Petr Leiman
Sent: 14 April 2011 16:23
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Femtosecond Electron Beam
People are looking into how to fit the old retired MeV microscopes with
pulsed electron guns (problem is there are very few of those beasts
left). If this works
@JISCMAIL.AC.UK] On Behalf Of
Petr Leiman
Sent: 14 April 2011 21:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Femtosecond Electron Beam
Dear Colin and all interested in the FEL development.
Please look at the figures in the first link I mentioned. Jom Luiten et
al. are able to record
Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Petr Leiman
Sent: 14 April 2011 21:07
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Femtosecond Electron Beam
Dear Colin and all interested in the FEL development.
Please look at the figures in the first
: [ccp4bb] Femtosecond Electron Beam
Colin,
We know that with a dose of 20-30 electrons per A^2, a lot of image
processing, and insane amount of luck, one can reconstruct cryoEM
images to 3 A resolution or better. A typical protein molecule is say
100 A in diameter, which is ~8000 A^2
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Petr Leiman
Sent: 14 April 2011 22:59
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Femtosecond Electron Beam
Colin,
We know that with a dose of 20-30 electrons per A^2, a lot of image
processing
11 matches
Mail list logo