HI
I am Research assistant at Physiology department of Michigan state university.
I am using the (ß-Catenin Antibody (Carboxy-terminal Antigen) #9587 for IHC on
mice femur sections. I have used this antigen unmasking method: For Citrate:
Bring slides to a boil in 10 mM sodium citrate buffer pH
Dear Tony and others,
Thanks you very much and to others for the very helpful suggestions. I
suppose there are many different ways to skin a cat!
I will take on board all the valuable suggestions and use them to optimise
my protocol.
With thanks and best wishes
Nick
-Original Message-
F
Nick,
Well after 30 years of doing ICCs, the worst results are nearly always
with tissues that have not been adequately fixed in formalin. This is
based on using over 300 different antibodies in both adult and pediatric
settings.
The following paper shows an example of what can go wrong with
unde
Hi Nicole,
Why bother dehydrating in ETOH then? Just rinse then air dry the
sections. I had the same problem recently and this is what I do now to avoid
the whole destaining problem. Once the slides are air dried, you can put
them directly in xylene.
Message: 12
Date: Thu, 4 Jun 2009 09:54:58
I answered off line however, we use Innovex Kits for animal tissue and it is
the best we have found. Using it with Background Buster for routine work
and the Fc Blocker for CNS tissue have given us reliable results we can
depend on.
Pam Marcum
UPENN Sch of Vet Med
New Bolton Center
-Original
Diana,
Robert S. Richmond (in an old Histonet post) remarks that "leprosy
requires control tissue containing the etiologic agent of the disease,
Mycobacterium leprae, which has never been cultured. It is an acid-fast
organism, but it does not have the same staining characteristics as
Mycobacterium
We have also had great results with it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly
Tuttle
Sent: Thursday, June 04, 2009 2:29 PM
To: histonet
Subject: [Histonet] Stat-Q Staining Kit
Does anyone
Yes, it absolutely does and more!! I have used it myself for many years in
my vet histology and am completely pleased with it's performance!!
Best Regards,
Loralei Dewe
On Thu, Jun 4, 2009 at 12:29 PM, Kimberly Tuttle wrote:
> Does anyone use the Stat-Q Staining Kit by Innovex for multispecies
Does anyone use the Stat-Q Staining Kit by Innovex for multispecies IHC? Does
it work as advertised?
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University of Maryland
Room NBW58, UMMC
22 S. Greene St
Baltimore, MD 21201
(410) 328-5524
(410) 328-5508 fax
This e-
FYI:
Leptospira is a spirochete that causes leptospirosis in animals and can
be transmitted to humans.
Leprosy is caused by the organism Mycobacterium leprae, an acid fast
organism best displayed by the Fite stain.
Thanks to all for your replies.
Diana Goodwin
-Original Message-
From:
Hello Netters
We have just changed over to the new DAKO stainer and we have several
anti-bodies RTU for the autostainer that we will not be using. All of the have
a 2010 experation date. If you are interested in them please contact me off line
AE1-3 2010-07 11ml
BCL22010-06 11ml
CD152
Greetings! Does anyone know of a source for M. leprae control slides?
Tried both HCS and AMT - both are out of stock with no re-stocking date.
Thanks!
Diana Goodwin
Supervisor, Anatomic Pathology
Pennsylvania Hospital
Philadelphia, PA
e-mail goodwind@ pahosp.com
The information contained i
Just a few ideas:
Do not cryoprotect unfixed tissue, it will just degrade.
Do not immerse the brain directly in isopentane. Remove brain from pup,
mount with a dab of OCT on chuck, put stem of chuck in -150 isopentane
cooled with liquid N2, brain will freeze in 30-60 seconds.If your chucks
are
Thank everyone for all the great info and assistance in solving my problem.
Paul, I am keeping you on my LIST for future repair needs.
The problem turned out to be a 'fixable' one. One of our grad students had
used the tome last week and adjusted the 'spring screws' behind the blade
holder? T
Dear Histoneters,
I am looking for help regarding flash freezing of rat pup brains
(Postnatal day 8). We need the tissue to be fresh (unfixed) for
cutting at the cryostat.
So far the technique has been:
1- to scoop the brains straight into cold (-50 C) Isopentane for
about 10 seconds,
2
Hello histonet this is a call for any advice.
I am a purely lab-taught histotech based in the UK and have been
studying part-time to enable suitable accreditation and for my final
year project am intending to use the large back catalogue of blocks in
the forensic lab I work in to study cardiac cha
There is no problem with that. The whole idea of washing with ethanol is to
differentiate and the section will be differentiated when no more color washes
out.
I don't know what artifacts you have but after sectioning there is nothing you
can do. You can prevent the artifacts while sectioning an
Hi Histonet... Quick question!!
I'm doing Luxol Fast Blue staining on frozen human brain sections (1% LFB in
95% EtOH with Hydroquinone/Sodium Sulfite Differentiator) and trying to
counterstain with Cresyl Violet. Following Cresyl Violet incubation (0.1% for
5') my protocol calls for me to dehy
I read somewhere that you are not to pH 0.175 M sodium acetate solution when
using it to prepare a nickel-DAB chromogen solution. The problem is that
whenever I make up 0.175 M sodium acetate (14.35 g of sodium acetate in 1 L
of dH2O), the pH of my solution is generally around 8.0 to 8.1. From what
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