Arthur Bolles Lee's The Microtomist's Vade-Mecum, 7th edition (1913)
is available online on Google Books, along with some other editions:
http://books.google.com/books?id=4m5MMAAJprintsec=frontcoverdq=arthur+bolles+leeei=BpJhS96xKJTIzASV9vnNBQcd=1#v=onepageq=f=false
This marvelous old
Delphine,
I think John is right about identifying the cells by shape. I do LCM, and I
perform a rapid hematoxylin stain just for orientation purposes. The total
time from fixation to air drying is 7 minutes. Any more time than that, and
the RNA is too degraded to get good results from
I would like to thank everyone that responded to my questions about these
scanners. These answers have been very valuable to my boss and I and will be
taken into account when we decide on which system to purchase!
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
Dear Colleagues,
At the risk of beating a dead horse, I would like to thank Bryan Llewellyn for
so eloquently and succinctly echoing my own thoughts on the matter that Dr.
Richmond commented on which has drawn criticism. For those who may have missed
it, I'll include it below my own meager
Hi - someone emailed me about my methodology, but I cant find the email, so
here it is:
I tried it @ 1:10, 1:25 and 1:50. All seem viable dilutions for the FFPE
xenografts. I used BIocare DIVA (EDTA) HIER in decloaking chamber, primary ab
for 2 hours @ RT, Biocare Rat-on-Mouse HRP polymer
Good morning fellow Histotechs,
Does anyone know of a supplier for the KP Maker Plus slide pens? Any
information on this would be very helpful thanks as I can not seem to locate a
vendor.
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Mercedes Medical
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of brent hart
Sent: Thursday, January 28, 2010 10:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Kp Marker Plus Slide Pen
- Original Message -
From: Christopher Pin c...@uwo.ca
Date: Thursday, January 28, 2010 9:22
Subject: Re: Stains for Macrophages for Laser Capture Microdissection purpose
To: Martin Sandig martin.san...@schulich.uwo.ca, John Kiernan
jkier...@uwo.ca, Paul Walton pwal...@uwo.ca
Hi all,
Yeah, that's it - that's where we got ours from. Plus the Mercedes Medical
rep called to remind me after seeing my post, so he does monitor the
Histonet... ;-)
--On Thursday, January 28, 2010 10:39 AM -0500 Blazek, Linda
lbla...@digestivespecialists.com wrote:
Mercedes Medical
Hi Nick,
If you can, grow the cells on thermanox cover slips (NUNC). Then process
them as normal for embedding in Eponate 12. I use acetonitrile in place of
the propylene oxide to avoid damaging the coverslip. When doing the final
embedding and polymerization, invert the coverslip over a drop
They last so long! That's why it's easy to forget where they came from!
-Original Message-
From: Maria Katleba [mailto:maria.katl...@stjoe.org]
Sent: Thursday, January 28, 2010 11:56 AM
To: Merced M Leiker; Blazek, Linda; 'brent hart';
histonet@lists.utsouthwestern.edu
Subject: RE:
These are the BEST pens I have ever bought for Histology! They last way longer
plus the writing is very black and the colour stays... even when you reprocess
a block
Yes... I mark my kids school supplies with them too!
Mercedes Medical 1-800-331-2716 www.MercedesMedical.com
These are
Kaye Ryan
North Florida Dermatology Associates
Histology Lab Supervisor
(904) 398-0547 Ext. 2004
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Thank you Kim, that does sound very promising, I still have not gotten
around to trying my sample of this, your work will be very helpful when I
do.
Best regards,
Patsy
Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 215
Aurora, CO 80010
Hi All,
I wanted to pass this link from the hotel on to you guys who will be attending
the Georgia Society for Histotechnology meeting March 26-28 this year. This
goes straight to the hotel and already has our code entered for you to reserve
your rooms at the Evergreen Marriott Conference
Dear Nick,
When I was growing and harvesting 3D cell cultures (spheroids grown in
matrigel) we would remove the culture medium, draw up the gel in a needle-less
syringe and place in to OCT embedding medium (Tissue Tek) before freezing in
Liquid Nitrogen. I would then cut 5-10um sections and
A researcher wants to measure the length of the intestinal crypts and asked for
a suggestion on what type of stain to use. I am thinking of using a
phloxine/tartrazine stain and do a double stain with a GMS to show the basement
membrane. What is your opinion on this?
Do you have other
Use mucicarmin stain. The crypt mucus will be evident and their sizes.
René J.
--- On Thu, 1/28/10, Perry, Margaret margaret.pe...@sdstate.edu wrote:
From: Perry, Margaret margaret.pe...@sdstate.edu
Subject: [Histonet] need help with stain
To: histonet@lists.utsouthwestern.edu
Samuri.
The website www.scienceheritagelimited.com has available 8 books that are
reprints of historically important history of microscopy and histotechnology
books included is the 1885 edition of Arthur Bolles Lee's The Microtomist's
Vade-Mecum. It's amazing how little (relatively)
I will be out of the office starting 01/28/2010 and will not return until
02/02/2010.
Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.
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Please don't take it personally.
If we are trying our best to meet our own high expectations then we do
not have to worry.
I believe that most of us do what Robert expects. This is supported by
my experiences at the recent NSH meeting in Birmingham Alabama. I was
privileged to meet some of the
Dr Richmond,
Thanks so much for that link to Vade Mecum. I've never before seen the book,
but it has brought me back to my childhood, when I found a similar
histotechnique book, Michael Guyer's Animal Micrology, in an antique shop my
mom was browsing, some 44 years ago. This book was
http://www.nesle2005.republika.pl/T6vJjAE12x.html
_
Hotmail: Trusted email with powerful SPAM protection.
http://clk.atdmt.com/GBL/go/196390707/direct/01/
A colleague from a neighboring university is trying to find both fine and
coarse honing compound for his knife sharpener. Does anyone have a good
source?
Thank you,
Jennifer MacDonald
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