Re: [Histonet] Red chroamgen/LeicaBond andBondMax

Yes; we run IHC tests everyday on our Bonds with Leica's "Red" detection kit. You do need to be careful when dehydrating the slides prior to coverslipping since the chromogenic product is not as stable as DAB. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD

Re: [Histonet] Histonet Digest, Vol 220, Issue 8

Well stated Tony, and thank for all this useful information. I am hopeful that we can get expiration dates overturned here in the United States. In the past, I have recommended a "Stability Guarantee" date to replace the "Expiration Date". After that date passes it would be the

[Histonet] Pneumocystis Control Tissue

I apologize someone contacted me several weeks ago about the availability of Pneumocystis control tissue, and I misplaced his name and contact information. Please contact me again if you are still in need of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology & The

Re: [Histonet] Dr Freida Carson

I'm very sad to learn of her passing. She was a remarkable person. I didn't know her well, but I always enjoyed speaking with her at meetings and learning from her. Many years ago I bought her entire collection of bound "Histotechnology" journals. Richard Richard W. Cartun, MS, PhD

Re: [Histonet] Microwave slide drying

I have never used microwave drying for IHC slides. I worry that if there is water trapped underneath the tissue section it might cause destruction of the tissue section if it is rapidly heated and "pops". You could run a "side-by-side" comparison and see if you notice a difference. Also, how

Re: [Histonet] release of body parts

We no longer release any tissue to a patient that comes to our Pathology Laboratory in formalin, and our Legal Department supports this decision. I know that our "Labor & Delivery" Unit has released placentas to patients; however, if the specimen doesn't come to Pathology, we don't get

Re: [Histonet] HSV1 and HSV2

Hi Martha: We are using type-specific monoclonals from BioSB (Santa Barbara, CA). They also sell a cocktail of the two antibodies which is what we use for screening. Rarely, do we get requests for type-specific identification. Richard Richard W. Cartun, MS, PhD Director, Histology & The

[Histonet] Question - Bone marrow core biopy specimens

Hello everyone: I'm wonder what your protocol is for cutting formalin-fixed, paraffin-embedded bone marrow core biopsy specimens? Do you cut a limited number of slides upfront and then go back to the block if the pathologist requests histochemical stains or immunohistochemical tests? We are

Re: [Histonet] Antibody diluent

Interesting . I will definitely check this out. I know I'm "Old School", but I have never "personalized" the diluent for preparing antibody dilutions. We have always used just one diluent for all the antibodies that we have run over my 43-year career. Richard Richard W. Cartun, MS, PhD

Re: [Histonet] Controls

I would recommend waiting at least 2 weeks after the case is signed-out in case additional testing needs to be performed, especially if the specimen contains cancer. And, I would always make sure that you leave representative tissue in the block meaning don't consume the entire specimen when

[Histonet] Formalin fixation for COVID-19 positive tissues .....

How long are you fixing surgical tissue specimens from COVID-19 positive patient's before tissue processing? I know that the CDC is recommending "72" hours for autopsy tissues, but, to me, that seems excessive for surgical pathology specimens. Any information that you can share on this

Re: [Histonet] BRAF testing

This is a very difficult target to detect. I was told recently by a vendor that one needs to use prolonged, very aggressive antigen retrieval (pressure cooker with high pH buffer) to get consistent results. Also, the type of cancer may influence your success (colon vs. thyroid vs. melanoma).

Re: [Histonet] IHC troubleshooting

Are you using some form of antigen retrieval? In addition to what has been discussed previously, antigen retrieval using heat (HIER) can cause tissue loss especially collagen. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic

[Histonet] Cellient for cell block preparation

Question - What has been your experience with Hologic's Cellient instrument for preparing cell block specimens? I understand that it is very good for pauci-cellular specimens. However, I am concerned about using these preparations for immunohistochemical and DNA/RNA testing. Thank you!

Re: [Histonet] PLAG1

Looking at an article in Histopathology (January 2018) on PLAG1 IHC testing, the authors used clone "3B7" (Novus Biologicals in Littleton, CO) on the Bond Max IHC platform form. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic

Re: [Histonet] Cell block processing

I agree with Joe. We used to use ETOH for cell blocks, but stopped using it when we started doing IHC biomarker testing on these specimens. Alcohol is good for some proteomic targets, but can be a disaster for others. We also fix all of our cell block specimens that are collected in saline

Re: [Histonet] Chromogenic ACD RNA ISH probes

We are currently running RNAScope for HPV and Albumin on our Leica Bond IIIs. It's a wonderful technology! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant

[Histonet] Tissue Contamination

What are people doing to ensure that there is no tissue carry-over on instruments between cases when grossing? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection

[Histonet] Major skin resections - CPT coding

I'm curious; how do you code major (complex) skin resections that also include soft tissue? These cases often have 10-30 paraffin blocks and lots of H slides. I'm thinking that they could be coded 88307 (simple resection) or 88309 (complex resection). I found out that our Dermatopathologist

Re: [Histonet] [External] Re: SDHB IVD antibody

ting, Angela K. [mailto:akbitt...@geisinger.edu] Sent: Tuesday, June 25, 2019 1:03 PM To: Cartun, Richard Subject: RE: [External] Re: [Histonet] SDHB IVD antibody EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders. Would you share your procedure? -Orig

Re: [Histonet] SDHB IVD antibody

Cell Marque offers an excellent SDHB rabbit monoclonal (clone EP288) that we use in our laboratory. However, it is not an "IVD". Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection

[Histonet] H Artifact

We had several cases last Friday that showed "optically clear nuclei" (primarily lymphocytes) on H Have you seen this artifact and, if so, what is it due do? I will be happy to e-mail a photo to anyone interested. Thank you as always for your help. Richard Richard W. Cartun, MS, PhD

Re: [Histonet] ER/PR controls

We use internal control blocks that we prepare from extra tumor tissue that we process after the case has been signed out. However, this is getting more and more difficult for us since most breast tumors are smaller now when detected (and that's a good thing!) which results in the entire tumor

[Histonet] Ventana p16 antibody

We use the Roche/Ventana p16 antibody (clone E6H4(tm)) on the Leica Bond Max. We are having a difficult time trying to order the antibody. Can someone verify the catalog number (705-4713) for me? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD

[Histonet] Question for Leica Bond IHC Users

We are preparing to install a Leica "Bond Stain Interface" which will allow us to move away from having to print slide labels manually for our IHC slides. I am not an expert on this so I am looking for help. We have "5" Bond IHC instruments that will be hooked-up to the controller; however,

Re: [Histonet] Derm IHC question

Doesn't sound like a fixation issue to me. Could the tissue be drying out before it's placed in formalin? Also, are these specimens inked for assessment of margins? I've seen ink interfere with immunoreactivity. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman,

Re: [Histonet] ER/PR question

If the tumor is ER/PR negative the first thing I do is to look for an internal positive control (immunoreactive benign breast epithelium). In my experience, the majority of these cases have internal positive controls to validate the negative ER/PR results. When I don't see internal positive

[Histonet] IHC Billing - 88342 vs. 88341

For those of you doing clinical IHC testing, have you been able to automate the billing of IHC (88342 vs. 88341) or do you still manual bill? Our IT staff wants to build-in a "88342" and a ""88341" for every antibody that we run and then have the pathologist select the appropriate one for

[Histonet] GD2

Hello everyone. Is anyone doing IHC testing for "GD2" on FFPE tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic

[Histonet] IDH1 Antibody

If you are doing IHC testing for IDH1 here in the United States, do you get the antibody (clone HO9) directly from Dianova in Germany or is there a US distributor? I did try HistoBiotech, but they no longer carry Dianova antibodies. Thank you. Richard Richard W. Cartun, MS, PhD Director,

[Histonet] Lyme Disease

Is anyone doing PCR testing for Borrelia burgdorferi (Lyme Disease) from formalin-fixed, paraffin-embedded tissue? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection

[Histonet] IDH1

For those labs that are performing IDH1 IHC testing on FFPE tissue, which clone are you using and where do you get it? Thank you. Happy Holidays to everyone. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory

[Histonet] Question - EM

How long can tissue remain in glutaraldehyde before EM testing is performed? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic

[Histonet] Alizarin Red Stain

Does anyone do the Alizarin Red histochemical stain for calcium in clinical samples? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director,

Re: [Histonet] Unstained slides

It appears that the presence of water, both endogenously and exogenously, plays a central role in the loss of antigenicity in stored unstained slides (see reference below). Labs that are experiencing significant loss of immunoreactivity in their unstained slides should check their tissue

[Histonet] Decalcification for Bone Marrow Biopsies

Help! For years we have used Decal-Stat for decalcifying our bone marrow biopsies with good results. For the past month we have been having problems with tissue loss and morphological damage with these specimens following decalcification. Unfortunately, this was just brought to my attention.

Re: [Histonet] LEF1 - antibody

We use a RTU (clone EP310) from Cell Marque on the Leica Bond. Antigen retrieval is "high pH" HIER for 20 minutes. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs

[Histonet] COnsult Reporting

I know I asked this question before, but I can't find my notes. If you provide a diagnostic consultation on pathology slides, do you need a separate report with a new "Date-of-Service" (the date that the consult is requested) for reporting and billing purposes? Thank you. Richard Richard W.

Re: [Histonet] Cold Ischemic interval

We are using the "ASCO/CAP" guidelines for all cancer resection specimens. Richard Richard W. Cartun, MS, PhD Director, Histology and the Martin M. Berman, MD Immunopathology and Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology

Re: [Histonet] Extra unstained paraffin embedded tissue slides

We file our unstains separately and then discard after one year. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford

Re: [Histonet] Beta-Amyloid antibody for IHC

No, I would like to use “6E10” if it’s still available. Thank you! Richard From: Colleen Forster [mailto:cfors...@umn.edu] Sent: Wednesday, December 27, 2017 4:23 PM To: Cartun, Richard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Beta-Amyloid antibody for IHC CAUTION

[Histonet] Beta-Amyloid antibody for IHC

We are looking for a good antibody to detect Beta-Amyloid in formalin-fixed, paraffin-embedded human brain tissue. Any recommendations? We have been using the clone "6E10" which we purchased from Signet Laboratories back in 2001. Thank you! Richard Richard W. Cartun, MS, PhD Director,

Re: [Histonet] ROS1

Yes, we've had excellent results using their clone "D4D6". We use it at 1:50 dilution on the Leica Bond Max following high-pH retrieval. Please contact me directly if you require additional information. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD

[Histonet] I am trying to contact ....

Diane Cadorette-Hall. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102

Re: [Histonet] EDTA decalcification tissue issues

We switched from acid decal to EDTA recently; primarily for molecular testing. We are starting to see some excellent results. At first, we were not decaling long enough (EDTA decalcification is a very slow process). We also use the Leica Bond Max and we have not had any problems with tissue

[Histonet] Announcing the 12th annual retreat of applied immunohistochemistry and molecular pathology at the beautiful Marriott, Key Largo, FL

I am forwarding this exciting meeting announcement to the Histonet Community. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology

[Histonet] Mass Spectrometry

Anyone working in Anatomic Pathology and using mass spectrometry for protein analysis on cancer cells? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs

Re: [Histonet] CD35

We are now using Epitomics' clone EPR6602 with great results. I don't know if it's offered as a "RTU", but I would check with Cell Marque or abcam; they carry Epitomics' clones Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic

[Histonet] ClearRite

Anyone using ClearRite (xylene replacement) for tissue processing? If so, what's been your experience? Any effect on IHC or molecular testing? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory

[Histonet] Question

We are thinking of changing the way we report consult testing (IHC, Flow Cytometry, and Molecular) for our system hospitals. Instead of accessioning the consult case here, the requesting hospital would create a "Procedure/Addendum" in Sunquest CoPath for their specimen and then send us the

[Histonet] IHC testing for Parvovirus (Human)

If you do this test, where do you get your antibody? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford

Re: [Histonet] Send-Outs

Do you have a Pathology Residency Program? If so, good luck with a policy that states that requests will be sent out in 48 hours. Our policy is 3-5 business days once we receive the request. Having said that, most requests are processed in 2-3 days. Richard Richard W. Cartun, MS, PhD

[Histonet] NGS Testing

I know this question is somewhat out of our domain, but are any of you engaged in "Next Generation Sequencing" (NGS) testing on patient FFPE cancer specimens? If so, what's your experience with insurance pre-certification and reimbursement? Thank you as always. Richard Richard W. Cartun,

[Histonet] Pathology Slides Send-Outs

I'm wondering if any of you take a patient's credit card to pay for the FedEx shipping of pathology slides when they request that their slides be sent to another hospital or medical institution? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD

[Histonet] ICD-10 Codes

What do you do when you receive a pathology consult and it is missing an ICD-10 code? I am being told that we cannot accession it until we get a code from the submitting doctor's office, and that we cannot add one. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The

[Histonet] IHC for GAB1 and YAP1

Anyone out there doing IHC testing for GAB1 and YAP1 to subtype medulloblastoma? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director,

Re: [Histonet] Return of Gross Only Specimens for Patient Use

I strongly discourage patients from taking their tissue out of the hospital. Once the specimen in placed in formalin it becomes a bio-hazard. We will release certain specimens for burial; however, the patient (or family) must contact a funeral home or mortuary to pick-up the specimen, and

Re: [Histonet] Immunoperoxidase Protocol on Cytospin

aryan, Naira [mailto:nmargar...@luriechildrens.org] Sent: Tuesday, March 14, 2017 3:24 PM To: Cartun, Richard Subject: RE: Immunoperoxidase Protocol on Cytospin cytoplasmic staining for Nodal protein. thanks ____________ From: Cartun, Richard [richard.car...@hhchealth.org] Sent:

[Histonet] Serotonin

Can anyone recommend a good anti-Serotonin antibody for diagnostic morphologic proteomics testing (aka, Immunohistochemistry)? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen

[Histonet] BRG1 IHC Testing

Is anyone doing clinical IHC testing for BRG1/SMARCA4? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford

[Histonet] IHC for ALDH1

Is anyone doing IHC testing for Aldehyde Dehydrogenase 1 (ALDH1)? Evidently, it is a prognostic maker used for breast CA. Happy Holidays everyone! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director,

[Histonet] Aluminum Testing

Has anyone ever heard of testing a tissue or body fluid sample for "accrued aluminum"? We have a child who has had multiple immunizations and the parents wanted the specimen tested. Unfortunately, none of this was communicated to us prior to the procedure being performed. Thank you and

[Histonet] Hard copy pathology reports

Since we are now using EPIC we will no longer be providing (mailing) hard-copy pathology reports to our clients effective January 1, 2017. Our healthcare providers now have access to all lab results and pathology reports in EPIC. Physicians can also be set-up for auto-faxing or can call

Re: [Histonet] ER/PR Uneven Staining

Can you provide more details about the uneven immunoreactivity? Are the negative cells located at the periphery of the specimen or are the negative and positive tumor cells mixed together throughout the specimen? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman,

Re: [Histonet] processing cell blocks in formalin

Yes, I will send you the procedure that we use. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour

Re: [Histonet] Billing Questions

I can only speak to IHC billing. My staff (now 2 people) in our IHC Lab does all the technical IHC billing manually. We average about 3,500 billable IHC tests per month now. The volume used to be higher, but our healthcare system sold most of our outreach business. Richard Richard W.

[Histonet] Formalin in Physicians' Offices

Do physicians' offices need to have procedures/policies "on-site" regarding formalin if they put patient biopsy specimens into the small formalin containers? What about formalin spill kits? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD

Re: [Histonet] Vacuolated and torn?

Microtomy is so important, but often over-looked. You might try giving the paraffin block(s) to different people and have them all cut H, and then have your pathologist compare them. If everyone's sections are suboptimal, then the problem is with fixation and/or processing. Richard Richard

Re: [Histonet] Kappa and Lambda ISH

Yes, the CAP now has a PT survey for HPV, EBV, and Kappa/Lambda in situ hybridization (ISH). Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director,

Re: [Histonet] CAP ANP.22970 Query

Do you participate in the CAP's PT program for ER/PR and HER2 IHC testing? If so, you can have all your pathologists who interpret ER/PR/HER2 IHC testing score the PT TMAs, complete the worksheets, and then you can establish their interobserver variability. If not, pull 20 cases where

[Histonet] Low temperature freezer

Our ancient "SoLow" -80 degree C. freezer died over the weekend. What do you recommend as a replacement? Do you prefer a chest vs. stand-up? We use it primarily for tissue storage. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology

[Histonet] C4d IHC in myocardial biopsy

Are any of you an expert with C4d IHC in myocardial biopsy tissue? We had a specimen this week that showed a pattern of immunoreactivity that I am not familiar with and I need to find out if this represents a specific disease process. Thank you. Richard Richard W. Cartun, MS, PhD Director,

[Histonet] Testicular biopsy for infertility

What are people fixing testicular biopsies in to evaluate infertility? In the past, I believe fixatives such as Zenker's and Bouin's were used for this purpose since they enhance nuclear detail. Obviously, those fixatives can no longer be used. Thank you. Richard Richard W. Cartun, MS, PhD

Re: [Histonet] P16

We purchase the antibody from Ventana and run it on our Bond Max platforms. During re-optimization, we found we could dilute it 1:10 (15 minutes) following "H2" (high pH) retrieval and still get excellent results. Richard Richard W. Cartun, MS, PhD Director, Histology and the Martin M.

[Histonet] FW: Question - CoPath

Make that "Sunquest" CoPath .. Richard -Original Message----- From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, May 24, 2016 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question - CoPath We will be switching f

[Histonet] Question - CoPath

We will be switching from Cerner CoPath to Sunrise CoPath in the near future (part of our Epic conversion). Is there anyone using Sunrise CoPath that uses a prostate diagram in their report to demonstrate the absence or presence of cancer in the different quadrants? Thank you. Richard

[Histonet] Date-of-Service for Consults

I have always maintained that the "Date-of-Service" for a pathology consult is the date that the pathology consult is initiated or requested. Is that correct? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory

Re: [Histonet] GLOMERULI ADEQUACY

We provide on-site assessment of the renal tissue obtained in Ultrasound, both at our main hospital and at our Children's hospital across the street. I handle probably 95% of the biopsies; I have two pathology colleagues (MDs) that handle the other 5% when I am not here. Occasionally, a

Re: [Histonet] Validating IHC on decalcified specimens

I have a validation file for ER, PR, and HER2 IHC testing on decalcified bone specimens with metastatic breast cancer. I compared the results with other specimens from the same patient (usually the primary breast cancer) that were not decalcified. Richard Richard W. Cartun, MS, PhD Director,

Re: [Histonet] tests on old cases

If the request is received more than "30" days from the original date-of-service, we re-accession the specimen, and bill and report the test result(s) under the new accession number. The requesting MD now becomes the submitting MD. Richard Richard W. Cartun, MS, PhD Director, Histology & The

[Histonet] Slide scribe

Years ago, I obtained a metal scribe for etching glass slides, but I can't remember which Histology company I got it from. Any suggestions? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory

[Histonet] IgG4

I am curious to hear which clone people are using to detect IgG4-positive plasma cells in FFPE tissue. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs

[Histonet] IgG4

Hi everyone. Which antibody (clone) are you using for the immunohistochemical detection of IgG4 in FFPE tissue? I just found out that the clone that we were using is no longer available. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD

[Histonet] Sunquest CoPath

Dear Histology Colleagues: I need your help once again. Our healthcare system is in the process of switching over to Sunquest CoPath; we currently have Cerner CoPath now. I have been told that when using their bar code and tracking system one cannot generate unstained slides (for possible

[Histonet] Shipping paraffin blocks overseas

It's been a long time since I have sent a paraffin tissue block out of the country. Can someone bring me up-to-date on requirements for shipping paraffin tissue blocks overseas (specifically Germany). I believe FedEx will be used for the shipping. Thank you! Richard Richard W. Cartun, MS,

[Histonet] H. pylori control tissue

Nancy Schmitt from Dubuque, IA asked about control tissue for H. pylori. I have tried e-mailing her, but my e-mail was returned as "Undeliverable". Nancy, please contact me. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic

[Histonet] Patient identifiers

Thanks to all who responded to my question on patient identifiers for prostate biopsies. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic

[Histonet] Patient identifiers

What are people accepting for the "two" identifiers on the small formalin bottles that are used for prostate biopsy specimens? Can you accept the patient's initials in place of the full name? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD

Re: [Histonet] Histology Workload

Dear Amy: Some of the companies doing molecular testing will pay you a fee for "specimen procurement/handling" since they realize the burden that these requests put on us. I suggest calling the company(s) that you deal with and see if you can work something out with them. And, you're

[Histonet] IHC Weekend Coverage

How many of you working in hospital-based pathology laboratories run IHC on weekends? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street

Re: [Histonet] Breast fixation

There are several studies (one listed below) that have addressed the issue of fixation past the recommended 72 hour maximum. In my opinion, the science behind these fixation recommendations is weak at best. In my own experience, I have seen no problem whatsoever going well past 72 hours of

Re: [Histonet] CMV antibody clone

We use DAKO's (an Agilent Technologies Company) product number M0854 (clones DDG9 CCH2). Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT

Re: [Histonet] Ventana antibody - H pylori

Is the Ventana antibody polyclonal or monoclonal? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax

Re: [Histonet] IHC turnaround time

I direct a busy and very efficient (I think) IHC lab staffed with 2.875 FTEs who spend most of their time doing IHC, but they also help Histology embed and cut paraffin blocks when needed (which is most days). For 2015 we are averaging 4,022 IHC patient test slides and 1,650 IHC control slides

[Histonet] FW: IHC turnaround time

I apologize; I meant to preface my remarks with, We have separate Histology and IHC Labs. My data may prove more useful to IHC only labs. Richard -Original Message- From: Cartun, Richard [mailto:richard.car...@hhchealth.org] Sent: Friday, June 19, 2015 11:21 AM To: Olszewski, Dawn

[Histonet] Specimen Accessioning .....

We have always accessioned specimens with multiple parts as 1, 2, 3, etc. and the corresponding paraffin blocks as A, B, C, etc. We are in the process of changing this to letters for the different specimen parts and numbers for the blocks. For those of you who do it this way, have you

[Histonet] RSV+ tissue

We recently performed an autopsy on a patient who died of complications related to Respiratory Syncytial Virus (RSV). We were able to label RSV in the formalin-fixed lung tissue using immunohistochemistry (or what I now call Morphologic Proteomics). I can provide unstained slides (maybe even

Re: [Histonet] Friday Trivia Question: Most specimen on a single case

The answer to this question will depend on what type of environment you work in. I work at a large tertiary care hospital with a major emphasis on cancer surgery and, as a result, we frequently get major cancer resections (with multiple parts) that generate well over 100 paraffin blocks per

[Histonet] Chlamydia control tissue

I have asked this in the past without any success, but I need to ask again, Does anyone have a control tissue for Chlamydia that they would be willing to share? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs

[Histonet] Pathology software

Can anyone recommend an inexpensive pathology software package for a small Dermatopathology laboratory? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street

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