[Histonet] IHC control blocks, DAB kit

Kristy, this might be of some help too: https://www.fixationonhistology.com/post/control-tissue-faqs [https://static.wixstatic.com/media/128854_2fda1d8f8d5c48cdad7701b3e866d885~mv2.jpg/v1/fit/w_1000,h_728,al_c,q_80/file.png] Control

Re: [Histonet] Reprocessing tissue

org> From: Perl , Alison Sent: Tuesday, September 3, 2019 12:42 PM To: Cassie P. Davis Cc: 'Cassie P. Davis via Histonet' Subject: [External] RE: Reprocessing tissue Warning: This email originated from the Internet! DO NOT CLICK links if the sender is unknown, and

[Histonet] Reprocessing tissue

Has anyone had success with just melting down unfaced, underprocessed blocks for reprocessing and sticking them in NBF for processing without taking them through the clean cycle? The senior tech is doing this and I am concerned about our processor getting paraffin in the NBF and other things.

[Histonet] Two part Special Stain wash

Hi Histonet folks, If you still have the NEXES special stainer do you use the two part wash cat#860-040 or the 10x concentrate cat#860-015? Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: cda...@che-east.org Confidentiality Notice:

[Histonet] Special Stain Wash

Happy Friday Histoland, I realize we've had quite a bit of discussion over the past couple of years about the Special Stain Wash not lasting as long and could possibly be why sometimes the reason the silvers don't turn out as nice as they used to on the NEXES special stainer. My

[Histonet] Retic staining

Hi Charles, in addition to the drop size clean glassware makes a big difference. I used to use new urine cups to ensure the silver was being made in a clean vessel. I hope this helps Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email:

[Histonet] Ventana p16 antibody

Dr. Cartun, your catalog # is one digit off, it may be from when they got FDA approval and changed the numbers. The catalog number for the "new" FDA approved p16 now labeled p16 HISTO BIOMKR (from mt printout) is 705-4793 Cassandra Davis Histology Technician AP Laboratory 302-575-8095

[Histonet] FYI- Roche Ventana users

Hi Histoland, I am biting my tongue HARD and just letting you know so it doesn't happend to you. I just got off the phone with Roche here is the heads-up. If one of their anitbody dispensers fails DO NOT put the antibody in one of their prep kits, as soon as you do they consider it off

[Histonet] possible answer

Reguarding: Hello fellow Histonetters I would like to ask you a question about IHC staining and derm cases. I am seeing a peculiar issue going on, where the melanocytes in the middle of the tissues are staining pretty well but when you get to the ends of the tissues either shaves or ellipses,

[Histonet] Eosin on processor for biopsies

Gareth, reguarding the eosin biopsy post on the histo net. Things you might want to considered looking at, I cannot say for sure because I don't know your processing solutions/schedule: Most Eosins are alcohol soluable, by putting the eosin in your formalin most of it will wash out during

Re: [Histonet] Input Request please

Good Morning Histonet, I am told our contract with Roche for the Ventana instruments will be up in March. Like many of you we have been having significant issues with our special strainers especially the GMS and are being told the same things you are as well as it's because

[Histonet] Input Request please

Good morning Histonet, I am told our contract with Roche for the Ventana instruments will be up in March. Like many of you we have been having significant issues with our special strainers especially the GMS and are being told the same things you are as well as it's because our

[Histonet] CAP checklist for temperature and humidity

Good morning folks, I need some help. You folks were wonderful helping with the optimal ranges earlier this year and I tried to find it before I asked but we are not "finding a specific CAP requirement telling us to monitor the humidity as well as temperature in the histology lab

[Histonet] AP LIS

We had CoPath and loved it, so easy to get stats, cases for controls tissue and nice looking reports Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: cda...@che-east.org Confidentiality Notice: This e-mail, including any attachments is

[Histonet] H for H. pylori

In the beginning of my last FT job we had a excellent female pathologist who could see the H. pylori on SurgiPath's H stain. I learned so much from her and dearly missed her toward the end. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email:

[Histonet] cellblocks

All our tissue is stained with the same H protocol. The difference maybe because cellblock are processed different to turn a fluid into a cellblock rather sampling a section of tissue. Most places fix tissue in NBF. Are using ThinPrep processing? If I recall correctly, the specimens are 1st

Re: [Histonet] histology lab temperature and humidity

in some our labs on mild foggy days, mainly in older buildings. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet@lists.utsouthwestern.ed

[Histonet] histology lab temperature and humidity

At one of my jobs we were discussing the way excess humidity can cause tissue to fall off slides even when they are charged or treated and may need to be in the slide dryer longer. Isn't there a CAP requirement requiring monitoring of the humidity as well as temperature in the actual lab not

[Histonet] slide dryer temperature monitoring

Hello Histonet Folks, does anyone know of a way to monitor the actual operating temperature of the slide dryer on automated stainers? Our area is very humid in the small state and were are/were having trouble with the top sections (1st level) on our slides falling off. We are

[Histonet] trying to track down a technique

Good morning Histonet Histology Folks, A few years ago a vendor was doing an onsite workflow analysis and showed us a video of a site that saved their unstained sections on paper rather than glass slides and if they needed the tissue later they were able to float the section off the

[Histonet] Special stains and hoods

25+ years in Histology, half the places I worked had vents on the wall near the countertop where we did specials, otherwise it was on the counterclosest to the sink. The only times we were time doing anything under a hood was for frozen section staining because of the fresh tissue, mixing

[Histonet] TMA for MMR panel validation

Happy Monday Histonet, I need your help with finding an appropriate TMA to use to validate the MMR panel. The stains are optimized, we just need an good TMA to validate it with. Thank you in advance for any help in this matter! Cassandra Davis Histology Technician AP Laboratory

[Histonet] ADH 5

Hi Histoland folks, Are/were any of you fine folks using the ADH5 from Biocare on the Ventana instrument? What are you doing now that the have replaced the panel with different antibodies which I already get from Ventana? My doctors really liked the panel, I have a couple of ideas

[Histonet] Iron counterstains

I enjoy this forum so much, I get to use brain cells I haven't used in years! When I saw your question about other counterstains for Iron Stain I kept thinking quick dip in Eosin (from you H) because I thought I remember having to do that in the 90's when we ran our of NFR. Histologist can be so

[Histonet] USA to Canada

Hello Histofolks, A friend asked if our HT/HTL are transferable to Canada I said no but that got me to wondering...what does a HT/HTL have to do to work in histology in Canada? Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email:

[Histonet] who cut the frozen

Allison, Here and the other three labs I worked at full-time, if the frozen arrived during "scheduled hisology hours", the histology techs cut them as long as it wouldn't accrue OT, after the techs left if the PA was still there, the PA would cut them , if the histology techs and PA left,

[Histonet] sample contamination

Response to NGS contamination: Hi Erin, Each point in Histology has a risk area and points of contamination prevention: Grossing- wash gloves, forceps and specimen sampling area between specimens Embedding- Wipe forceps, fingers or gloves if you wear them while embedding and embedding

[Histonet] tissue banks

Good afternoon Histology Folks, 10 or more years ago there used to be a couple of companies that would buy body fluids and tissues from labs that were ready to be disposed of. Do they still exist and would you please share their contact information? thanks, Cassandra Davis

[Histonet] Histology lab flooring

Hi Jennifer, I've worked in five labs all with different flooring and the floor that is in my current lab seems to fit your needs. It is a battleship gray deck paint (with sand or grit in it) that was applied when the lab was built 1990. The gray hides the dirty paraffin, lets you see

[Histonet] Billing question help please

Good morning Histoland, our supervisor came in asking about an regulation she was told about this morning that says no more than 9 IHC can be billed on a patient per day. Can some one help me find a resource for this? Cassandra Davis Histology Technician AP Laboratory 302-575-8095

[Histonet] Histology Shortage?

I am not sure about overseas but the New Castle County does not have a shortage. There is a school in Delaware that gradutes 5-6 students a year. Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: cda...@che-east.org Confidentiality Notice:

[Histonet] Roche-Ventana Special Stainer

Good morning All, My supervisor asked me to get opinions on the newer stand alone automated special stainer from Roche-Ventana is now offering so I am asking. We currently have the 2009 countertop model. Thank you in advance for taking you time to respond. Cassandra Davis Histology

[Histonet] Ventana p16

The Ventana p16 isn't really being discontinued. They just got FDA approval for it, this approval is using the Optiview DAB kit only. They are changing the catalogue # for the lots since the approval . Your sales rep. should provide information if your are getting it from them. Cassandra

[Histonet] Questions RE: "what I have found about p16" and cellblocks for controls

Is there anyone in Histonet land who is using both the Optiview and Ultraview on the XT and ULTRA at the same time? I'd like to ask a few questions, please email me. We have a cytology cellblock case that came out stunningly positive for Her2 and one of our pathologist thought it would

[Histonet] what I have found about p16

Hi Histonet folks, Thank you for all the help, for those who are following what I have found: (1) Sigma-Aldrich does have p16 (it is also know as INKa): Anti-INKa(p16) antibody, clone 13H4.1 cat # MABE1328 or Anti-p16 Antibody, clone D25 cat#MAB4133 (2) Stacy was

[Histonet] p16 replacements?

Hi Histonet Folks, our current vendor will be discontinuing carrying the antibody for p16. Does anybody have suggestions for replacing in it? We currently use it to stain for CIN. Many thanks in advance for sharing. Cassandra Davis Histology Technician AP Laboratory 302-575-8095

[Histonet] THANK YOU for responses CLIA and Histology

Thank you to all who messaged me about this! Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: cda...@che-east.org Confidentiality Notice: This e-mail, including any attachments is the property of Trinity Health and is intended for the sole

[Histonet] CLIA classifications

Hi Histofolks, We are having a friendly discussion as to the CLIA classification of job titles & I could use some help clarifying: Histology microtomy & H/E- I'm say it would be moderate complexity others are arguing otherwise... Cassandra Davis Histology Technician AP Laboratory

[Histonet] clip-on magnifiers

RE: clip-on magnifiers Try Ted Pella Cassandra Davis Histology Technician AP Laboratory 302-575-8095 Email: cda...@che-east.org From: histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Tuesday, October 18,

[Histonet] Plastic embedding and Microtomy

Hi Daniel, I haven't seen plastics embedding and microtomy since the mid 90's. When I was at CCHS in Newark, DE we used to do our bone marrow biopsies in them. At that time there were molds big enough to embed a penny in. Yes, you need a different microtome to section these

[Histonet] Eosin to help see tissue better

Hello Rebecca, Reguarding the following message: "I had a biopsy today that was nearly impossible to see on the sponges during embedding or in the block. I've heard mention of marking these with eosin to make them easier to see. Has anyone done this? Or do you use some other type of

[Histonet] Histology Medical Waste

In May, Curt sent this out to Histoland: "Message: 2 Date: Wed, 11 May 2016 20:18:52 + From: Curt To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] embedding and microtomy "medical waste" Message-ID:

[Histonet] Static electricity Tip

I cannot be the only histology tech that had a problem with static electricity so I am sharing my recent find. In the past I had tried extra fabric softener, dryer sheets at microtomy, wool balls in my dryer with my uniforms, any suggestions, I tried with little to no success. The recent try

[Histonet] Clinical Lab vs. Antomical Pathology

Karen, I agree with Lester, you need support. Unfortunately, histology often gets "lumped in" with clinical laboratory medicine (in my experience) and our leadership is often a med tech. I have nothing but sincere respect for medical techs, anatomical pathology is just a different

Re: [Histonet] H & E Troubleshooting

with bleached containers, but when that happened everything look light. Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare 603-524-3211 ext: 3220 -Original Message----- From: Cassie P. Davis via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, February 23, 2

[Histonet] H & E Troubleshooting

Hi Histo folks, We are having a fun time troubleshooting our H because the problem in not consistent... Yesterday we poured fresh xylene, Hematoxylin , Eosin & bumped our alcohol since our ordered had not arrived yet. One of our doctor said our hematoxylin & eosin was readable but