[Histonet] 5 Yr old Leica ST5020 is down

oller board" for $17,600, stepper motor for arm, etc. with total quote for over $23,800. Has anyone else had major issues with this instrument over time. We are trying to decide whether to fix it or go for a new stainer. I'm kinda wondering if we got a lemon. Thank you for your time, Cath

[Histonet] TUNEL on Leica Bond

Hello Histo-friends, Is anyone doing a TUNEL stain on any of the Leica Bond instruments? I will be attempting to set this up and thought I would reach out first. Thank you, Cathy Cathy M. Mathis Laboratory Technician IV -Original Message- From: histonet-requ

Re: [Histonet] Leica Bond Max artifact

. If anyone has a solution to the problem, I would also be interested. Best to all, Cathy Cathy M. Mathis Laboratory Technician IV Department of Pathology Section on Comparative Medicine - Clarkson Campus Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.1538 \  f 336.716.1515  cmmat

[Histonet] Questions concerning Leica Bond RX

all for your time, Cathy Cathy M. Mathis Laboratory Technician IV Department of Pathology - Section on Comparative Medicine Clarkson Campus  Medical Center Boulevard  \  Winston-Salem, NC 27157 p 336.716.1538 cmmat...@wakehealth.edu -Original Message- From: histonet-requ

Re: [Histonet] CD34 for primate tissue

This is a great website for NHP info. Thank you all for all your wonderful help!! Hope everyone has a great 4th of July weekend Cathy -Original Message- From: histonet-requ...@lists.utsouthwestern.edu [mailto:histonet-requ...@lists.utsouthwestern.edu] Sent: Friday, July 01, 2016 1

Re: [Histonet] CD34 for primate tissue

are the same clone as the companies that say it does. I guess I will just break down and purchase a different clone that states it works in rhesus monkey. Cathy -Original Message- From: Eddie Martin [mailto:edmarti...@gmail.com] Sent: Wednesday, June 29, 2016 8:22 PM To: Cathy M. Mathis

Re: [Histonet] CD34 for primate tissue

protocols work, just not on my rhesus. Does anyone know of a CD34 that will work in this species? More suggestions please? Thank you all for your time. Cathy -Original Message- From: Eddie Martin [mailto:edmarti...@gmail.com] Sent: Wednesday, June 29, 2016 5:34 PM To: Cathy M. Mathis

[Histonet] CD34 for primate tissue

retrievals from Leica. Now I am try epitope retrieval offline; pronase, trypsin, pressure cooker with high and low pH solutions. Still no signal. Any help would be greatly appreciated. Cathy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] working Dako Autostainer, OR/WA area make offer

want to crate it up, but if there is someone in the Portland Oregon area who wants to come, package it, and take it off of our hands feel free to make us an offer. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292

[Histonet] RE: Histonet Digest, Vol 133, Issue 13

Are you filtering your trichrome stain just before using it? Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, December 11, 2014 1:07 PM

[Histonet] RE: Gomori Trichrome

Have you tried filtering the stain just before using it? Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, December 11, 2014 1:07 PM

[Histonet] Part time histotech needed in OR

We just posted a part time HT position at Tuality Community Hospital in Hillsboro, Oregon. Go to Tuality.org under careers to apply. Tuality has a great pathology lab that is only open M-F. Position is for 24 hours per week with benefits. Cathy Crumpton HT(ASCP), Lead Histotechnician

[Histonet] Setting up ASR antibody to meet CAP requirements

characteristics... in accordance with the Method Performance Specifications section of the All Common Checklist. Is there anyone who would be willing to share their procedures on verifying and validating ASR's for IHC purposes? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community

[Histonet] lower glands in colon not staining with IHC

as to something else that could be causing a lack of staining on the internal colon portions? We use Dako reagents and polymer kit and the skin and other tissues in the sausage block stain beautifully. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292

RE: [Histonet] bone marrow aspirations

on the quality of the slides. Cathy Kelowna, B.C. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Shea's Sent: Saturday, June 1, 2013 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone marrow

RE: [Histonet] AutoTech Embedder( Sakura)

I am also interested in information on the Sakura AutoTech Embedder. My manager has put on the capital equipment list along with the Sakura Express processor. Thanks, Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

[Histonet] PIN4 staining

We use Bond slides and have noticed that occasionally they seem to have an extra thick coating on them that will pick up DAB and turn slides brown. If the glass itself is turning brown this might be your issue. Have you tried different slide lots or brands? Cathy Crumpton HT(ASCP), Lead

[Histonet] Sakura glas coverslipper and mounting media types

after just a few days of storage. BTW the oven is at 40 degrees and isn't boiling hot to dry the slides. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] IVD P63

I was just told by my Biocare rep that today was the last day they are selling P63 antibody. I would like to purchase IVD P63 from another vendor, who else sells it? I have having a few problems finding good info just using Google. What is your lab using for diagnostic purposes? Cathy

[Histonet] IVD P63

concentrates. What a racket! Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Patient requests for tissues

everyone safe. Do you allow patients to get back their specimens after the case is signed out or not??? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: Interview Questions

who was qualified. You could tell quite a bit about their knowledge just from the written portion and people seemed less nervous when there wasn't a person hovering over them. Our hospital sees this skills test as the equivalent of a typing test for transcriptionists. Cathy Crumpton HT(ASCP

[Histonet] Population Served compentency requirements

not work with the public, just their specimens. Any ideas??? Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

[Histonet] Histology lab assistant in CA

be a real asset to your team. Cathy Crumpton HT(ASCP), Lead Histotechnician Tuality Community Hospital 503-681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] repetitive wrist injury

been any suggestions yet. Cathy Kelowna, B.C. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Part time postition for histology lab assistant

department. The hours are M-F 12-5 pm. Cathy Crumpton HT(ASCP), Histology Lead Tu= ality Community Hospital Hillsboro, OR 97123 (503)681-1292 = /FONT References 1. 3Dhttp://www.Tuality.org/ ___ Histonet mailing list Histonet

RE: [Histonet] eosin

not had a complaint about the eosin interfering with FISH from our referral lab. Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital _ From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histot

RE: [Histonet] Need opinions on HE stainers please!

but once that was corrected it has been working well since then. The only draw back that I can see with the attached coverslipper is that the slides take longer to dry than with the older model. The slides are kept horizontal rather than vertical so the xylene doesn't run off. Cathy Fitzpatrick

[Histonet] CAP question ANP.22970

the pathologists in the laboratory..= . Do you have a separate computer program or do this by hand? = DIV Cathy Crumpton HT(ASCP), Histology Lead Tuality= Community Hospital Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list

[Histonet] Re: Histonet Digest, Vol 83, Issue 19

. They j= ust sit them on the counter and they cool - just like they were being embed= ded. When we come in on Monday we just sit them in the embedding cent= er for 15 minutes and they warm up enough to start embedding. No harm= to tissues and no one has to come in. Cathy

RE: [Histonet] problems with staining IHC

with the lack of progress in solving the problem. As anyone noticed that the Plus slides don't seem to have the same adhesive properties that they used to? Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital Kelowna, B.C. -Original Message- From: histonet-boun

[Histonet] Skills testing for a histo lab assistant

there do this f= or their new hires? What and how do you do to test? nb= sp; Cathy Crumpton HT(ASCP), Histology Lead Tuality Community = Hospital Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet

[Histonet] Technical Assistant or plain Lab Assistant?

I need help from my fellow histotechs. We are creating a po= sition for a histology lab assistant. We need someone to assist the p athologist when grossing (not grossing themselves), clean machines, file, d= ata entry and accessioning, etc. We are trying to decide if

[Histonet] Attention new equipment distributors

such as this but of course time and costs have changed things. Please respond off line to camay...@gmail.com Thanks in advance, -- Cathy Mayton ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] xylene in an unventilated area

a similar set up? The= y share their area with micro and core lab and others are complaining about= fumes even though they have borrowed a mini-hood from us. Badge resu= lts are still pending. Cathy Crumpton HT(ASC= P), Histology Lead Tuality Community Hospital Hillsboro, OR 97123

[Histonet] attention equipment resellers/refurbishers

to interested parties. -- Cathy Mayton ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Biocare P63 with Dako Flex detection

). A dilution with the same protoco= l at 1:100 is way too light. Thanks, = /DIV Cathy Crumpton HT(ASCP), Histology Lead Tuality Community Hosp= ital Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet

[Histonet] Can anyone use some reagent?

off= list. Cathy Crumpton HT(ASCP), Histology LeadTuality Community Hospital Hillsboro, OR 97123 (503)681-1292 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Ventana Ultra

We are in the process of upgrading our immuno stainers to the Ventana Ultra. I am interested in how people validate a new instrument and how the Ultra was integrated or changed lab workflow. Cathy Kelowna, B.C. ___ Histonet mailing list Histonet

[Histonet] liquidating lab equipment

(model 4240) 5) Exakt grinder all of the equipment was being used right up to the last week we closed the lab -- Cathy Mayton ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Superfrost Plus Slides

and distributed to vendors under their own name. Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fahy, Claire Sent: Tuesday, March 16, 2010 4:05 AM To: Lynn Lee Cc: histonet@lists.utsouthwestern.edu Subject

RE: [Histonet] Downtime Procedure

. The downtime log sheets were very useful but I'm sure there is much more that we could be doing. Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Green JumpyOne Sent: Tuesday, January 19, 2010 8:57 AM

RE: [Histonet] maximum block cutting

Does this average include trimming into the block or just the actual cutting of the section? Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, December 10, 2009 5:55 AM

RE: [Histonet] Automated HE stainers

they are staying on the carousel until they are mostly dry. Cathy -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen L. Sent: Monday, October 12, 2009 5:42 AM To: Connolly, Brett M; histonet

[Histonet] double immunostaining with antigen retrieval and no antigen retrieval

that doing antigen retrieval for antibody that doesn't require it gives us non specific staining. I would appreciate some advice. Thank you very much. Cathy Malcontenti-Wilson ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

RE: [Histonet] immuno staining

and they were sold under multiple vendors. Good luck with tracking down your problem. Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital Kelowna, British Columbia Canada -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

[Histonet] Tissue Tek II- last one

Almost have the lab boxed up but I have 1Tissue Tek II staining tray with 12 staining dishes along with 14 more other staining dishes left. Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 ___ Histonet mailing list Histonet

[Histonet] Tissue Tek II gone

Thanks for all the inquiries but the Tissue Tek II staining tray and all the extra dishes are gone. Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman

[Histonet] hand held pH meter for sale

I have a Fisher Scientific Accumet hand held pH meter for sale. Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] wood slide boxes for sale

I have 5 nice wood slide boxes that hold 100 slides for sale Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] HI Downdraft fume extractor for sale

I have a HI downdraft fume extractor for $50 and we will provide carbon to refill the drawer. Cathy A. Mayton Wasatch Histo Consultants, Inc. 775-625-4425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

[Histonet] breaking glass jar/vials for MMA embedded specimens

shards of glass. This is a great way to vent frustration!! Cathy A. Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] small equipment for sale

Leica DME dual head microscope has 4x, 10x, 20x and 30x and 40x, used for QC of staining and final QC of slides Cathy Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 ___ Histonet mailing list Histonet

[Histonet] 5 gal MMA for sale

I have a sealed 5 gallon can of Aldrich MMA for sale for $50 plus shipping. We also have 12 or 13 gallons of Stat Lab Reagent Alcohol. Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 ___ Histonet

[Histonet] light box for sale

I have a light box for $50 plus shipping and a nice Shandon paraffin embedding center. Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] glassware + Tissue Tek II staining trays

I have glass dishes, glass staining racks, beakers, flasks and 3 Tissue Tek II staining trays. I also have lots of the slide holders for the Tissue Tek II staining trays. Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 www.wasatchhisto.com

[Histonet] paraffin slides for sale

Fisher Scientific slides @ 2 $2/box 20 boxes green 5 boxes yellow Premiere brand 20 boxes green 8 boxes white Cathy A. Mayton Wasatch Histo Consultants, Inc. 80 Youngberg Road Winnemucca, NV 89445 775-625-4425 www.wasatchhisto.com ___ Histonet

[Histonet] plastic slides for sale

white769 slides 2 x 2 x .060 clear88 slides 2 x 2 x .060 white 112 slides higher quality plastic slides 2 x 3 x .030 clear346 slides Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet

[Histonet] more plastic slides

found some more plastic slides 2 x 4 x 060 clear164 slides These we used to true the head on the Exakt grinder. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Isocut cutting fluid

Free to good home 6 bottles of 950 ml Buehler Isocut cutting fluid This was sent to us accidently by Buehler and we never used Just pay shipping and it is all yours Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet

[Histonet] cassette storage/cardboard slide holder for sale

For Sale 2 set of paraffin block drawers $25 20 or better cardboard slide holders (holds 20 slides) $1 each Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

[Histonet] For Sale small lab equipment

dessicator lots of consumables Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Sanderson's Rapid Bone Stain

ground sections in the RBS, rinsed in water and then counterstained. As Gayle mentioned, intensity of the RBS or the counterstain can be increased with addtional time. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet

[Histonet] free Tissue Tek II vacuum infiltrator

I have an old Tissue Tek II vacuum infiltrator free to a good home. Only cost will be shipping via ground..either FedEx or UPS. Cathy Mayton Wasatch Histo Consultants, Inc. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Cathy Crumpton is out of the office.

I will be out of the office starting Mon 03/02/2009 and will not return until Thu 08/27/2009. If you have any histology concerns please route them to Connie Basinski during my absence. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] uncertified histotechs

are quite pleased  with my work.   Cathy Boyd HT(ASCP) Beaufort County Medical Center Washington, NC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] using patient blocks for positive IHC controls

Does anyone know if there are  any regulations on how much tissue you can cut from a patient block?  We currently use known positive patient slides for our IHC positive control slides?  One of our pathologist questioned if we could use an entire patient block ,cutting through the block for