[Histonet] Tissue Adherence Issue

one hour) and carefully handled. Superfrost Plus slides are used. Especially gentle heat for heat induced antigen retrieval. Try to use enzyme antigen retrieval as possible. Best luck. Dorothy Hu > Message: 2 > Date: Wed, 17 Jul 2019 09:21:49 -0500 > From: "Knutson, Deanne" > To: &quo

Re: [Histonet] [EXTERNAL] Deplastifying MMA

Hi Lori, Would you please tell me which company and catalog # of tetrahydrofuran you used before? For how many minutes you did for deplastifying in tetrahydrofuran? Thanks in advance. Dorothy Message: 3 Date: Wed, 14 Feb 2018 20:05:16 + From: "Garcia, Lori, M.Sc."

[Histonet] Deplastifying MMA

> > Hi Reuel > We had the same thing as you. It was caused by bad MMA, I tested. Write down the lot # and ask refund. Test new lot # 1st of MMA in the future. It was very frustratingI know. Best luck. Dorothy > Message: 2 > Date: Mon, 12 Feb 2018 21:07:15 + > From: Reuel Cornelia

[Histonet] EDTA decalcification tissue issues

, undecalcified bone now. If anyone has research paper regarding this, please share. Thanks. Dorothy Hu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cut FFPE Slides for ISH

? Fresh cut section will yield more signal for sure. I think as long as you have a few copies of genes in your tissue, RNAscope should be able to detect. Dorothy Hu HSDM > Message: 2 > Date: Mon, 28 Aug 2017 15:30:08 + > From: "Jennifer Phinney" <jh...@vet.k-stat

[Histonet] KOH softener to "decalcify" bone?

I know someone used KOH with EDTA together to decalcify bone. It is worthwhile to try KOH only to soft bone and kept dynamic labeling. If anyone had tried successfully, please let us know. Thanks. > > Today's Topics: > >1. Fabric softener to "decalcify" bone? (Angela Lamberth) >2. Re:

Re: [Histonet] Histonet Digest, Vol 157, Issue 13 Kawamoto's film method (Jamie McGinness)

Please check this web for the answer of your question. http://section-lab.jp/English/Referece%20E.htm Dorothy Hu > > > I am looking into trying the Kawamoto's film method. ?I have the order sheet > for the cryofilm sheets and other supplies. ?I did have a few questi

Re: [Histonet] cryosectioning undecalcified samples (Caroline Miller)

Hi, I compared cryojane with Kawamoto's method. I prefer the later. Please see the following paper. Arch Histol Cytol. 2003 May;66(2):123-43. Use of a new adhesive film for the preparation of multi-purpose fresh-frozen sections from hard tissues,

Re: [Histonet] Histonet Digest, Vol 147, Issue 21"............Double stain IHC question

I wonder if streptavidin Alexa (495 or 488) can be use to bind to biotinlyted primary. So no worry about species issue? May need to do biotin blocking first to get ride of endogenic biotin I guess. Dorothy Hu Sent from my iPad > On Feb 20, 2016, at 1:00 PM, histonet-r

[Histonet] Decalcification with formic acid sodium citrate

There was a paper http://www.genedetect.com/Merchant2/ExampleRefs/Decalcifying_protocols.pdf Talking about formic acid (Morse solution) can get as good result as EDTA in ISH. FYI. Dorothy Hu Today's Topics: 1. Re. Decalcification with formic acid sodium citrate (Gayle Callis

[Histonet] TRAP Stain Help

media. You have to use water based mounting media. I don't use Sigma kit (tried once), but my protocol is more sensitive. We do trap on both paraffin and MMA sections. If you need I can forward you the paper. Dorothy Hu HSDM Message: 2 Date: Mon, 11 May 2015 20:26:14 + From: Wait, Trevor

[Histonet] Bubble in MMA blocks

to close the cap and leave the vial in water bath and space out to vent the heat generated during polymerization. But how can I say the vacuum dessiccator. Thanks everyone to explain. Dorothy Hu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Decalcification using EDTA

It is optional to decal in 4oC, but it is much better for preserve antigenicity and get good result from enzymatic staining. Similar way as you do fixation, you can choose RT or 4oC. Dorothy Hu Hello Histonetters Is it imperative OR optional to maintain samples at below 4 degrees celcius when

[Histonet] mice tibia histology

is because 1. temperature too high?; 2. too long in xylene? hope this help. Dorothy Hu Hi does any one doing mice tibia histology, we use to fix in formalin for 24 hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 24 hours long protocol in automatic processor. But I am

[Histonet] formic acid treatment and alpha-synuclein staining (Tyrone Genade)

.hul.harvard.edu/pubmed/?term=Hansen%20L%5BAuthor%5Dcauthor=truecauthor_uid=10663973 , Masliah E http://www-ncbi-nlm-nih-gov.ezp-prod1.hul.harvard.edu/pubmed/?term=Masliah%20E%5BAuthor%5Dcauthor=truecauthor_uid=10663973 . Hope this wil help. Dorothy Hu HSDM Message: 3 Date: Fri, 10 Apr 2015 15:00:55

[Histonet] Kawamoto methods

Try this http://www.section-lab.jp/. Just email them and ask how to order. Dorothy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] cutting MMA cross section of mouse femur

especially from hard tissue committee, please help. I'm very much appreciated. Dorothy Hu MGH endocrine ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: Histonet Digest, Vol 131, Issue 26

*Regarding processors* *I also need to help on determine which processor for our core. Our majority tissue is bone. * *Right now, we like VIP serials because of vacuum/pressure plus agitation. Please help me* *on this VIP processor, I never used this kind one. We are very small volume* *core,

[Histonet] Protocol of MMA Plastic section TRAP staining

(Sigma, F3381) with above solution and filter. Stain slides @37oC for at least one hour. The solution can be use for one month when stored in 4oC. Continue to do counterstain and mount slides. Thank you. Dorothy Hu MGH Endocrine histocore Email: d...@partners.org

[Histonet] Re: Histonet Digest, Vol 130, Issue 16

Hi Dear Histoneters, Can you tell me which kind of Brady labeler you are using? I want to get one. Do you have catalog number? Many thanks. Dorothy MGH Endocrine histocore I use the Brady system and I like it a lot. I work in a small animal diagnostic lab and will have 60-80 blocks on most

[Histonet] Where do you order MMA for undecalcified plastic sectioning mouse bone?

which company do you ordered MMA? Sigma has back order, so I try to find reliable resource here. Thanks, Dorothy abt...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Help on get PDF file paper from J. Histotechnology

Hi Histonetters, Would you please let me know why I can not get PDF file from J Histotechnology any more? I renewed my membership after new year and changed my password since i don't remember my old one. I can get in Maney Online for only Abstract, but not whole article. The paper I tried to

Re: [Histonet] Help on get PDF file paper from J. Histotechnology

[mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dorothy Hu Sent: Friday, January 17, 2014 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help on get PDF file paper from J. Histotechnology Hi Histonetters, Would you please let me know why I can not get PDF file

[Histonet] Re: Do I have to destabilize MMA?

Thanks Jack's answer. I though I shouldn't to destabilization step as well. But my boss wants to do it. Is there a chemical theory behind this? Why previous protocol has this step (it must be reason)? And why the step is skipped now? I wish I know before approaching my boss. Additionally, there

[Histonet] Calcein labeled plastic section mounting procedure

color fading? Much gratitude. Dorothy Hu abt...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet