Thank you for your help. This is encouraging.
Helen
-Original Message-
From: Glover, Kimberly [mailto:kimberly.glo...@polysciences.com]
Sent: Tuesday, March 22, 2016 11:38 AM
To: Helen Fedor <hfe...@jhmi.edu>
Cc: histonet <histonet@lists.utsouthwestern.edu>
Subject:
Hello, I did try and search the archives to see if there was any advice on this
but didn't find an answer.
Do you know of a good way to fix H slides that had plastic coverslips where
the coverslip is lifting from the slide and in some cases coming completely
off? In all cases the tissue is on
Hello Bernice, we have tried out several and found that enercell works.
CR2450 their number is 23-808.
WE might have found them on amazon.
Cannot remember.
Helen L. Fedor
Oncology Tissue Services, Manager
Johns Hopkins University
411 N. Caroline St
Room 310 Basement| Bond St Annex Building
Hello, We have the Pathology Devices Semi automated units. We are happy with
them. Our decision to keep with the less automated units are largely due to our
work flow. We make TMA's for customers. But the customer has to do all of the
work in the design and data entry into out TMAJ Database. I
Hello all, I would be interested in pros and cons of using one of the Rapid
vacuum processing units for processing of biopsy tissues. Is this technology
improving the processing? Any specific problems that may be occurring.
Thanks in advance.
Helen L. Fedor
Oncology Tissue Services,
Hello, I believe that the products for the CryoJane tape transfer are still
available from Fisher.
Helen L. Fedor
Oncology Tissue Services, Manager
Johns Hopkins University
411 N. Caroline St
Room 310 Basement| Bond St Annex Building
Baltimore, MD | 21231
410-614-1660
Hello, we recently purchased the Primera unit from Creative waste solutions.
http://www.cwsincorp.com/
we like it. I would be happy to discuss it further if you like.
Helen L. Fedor
Oncology Tissue Services, Manager
Johns Hopkins University
411 N. Caroline St
Room 310 Basement| Bond St Annex
We had the PTL, which is a little more cumbersome. We are now using the BBP33,
and are very happy with it.
Helen L. Fedor
Prostate Tissue Bank, Manager
Oncology Tissue Services, Manager
Johns Hopkins University
600 N. Wolfe St, | Marburg Room 406
Baltimore, MD | 21287-7065
410-614-1660
We are also using the Brady labeler. It is versatile, easy to use, they have
great tech support. We are not using the cassette label attaching machine. For
the cassette printer we are using the Primera. The Tech support there is also
great.
Helen
-Original Message-
From:
Hi, I think that it is not necessary to actually get them to roll. We just
collect all of the sections and put them into the tube. Scrunched, not rolled.
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
Hello, We are in search of a temporary histo-tech or histologist to work in our
OTS Core lab. Primarily we do research work to support the efforts of the
Cancer Center Investigators. A majority of the work is on animal tissues.
Gross, Process, Embed, section and do recuts, i.e., lining up
Making cell tissue blocks
Cells:
. Harvest and fix in formalin* for at least 3hrs or overnight (10 fold
more formalin)
. HAVE A NICE VISIBLE SIZE PELLET (1 confluent T75 minimum or 1 T175)
. Remove formalin* and wash 2x's in cold 1x PBS
. Cover cells with some 1x PBS and
You can tell it is Friday.
:)
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cristi Rigazio
Sent: Friday, September 20, 2013 1:40 PM
To: Davis, Cassie
Cc: histonet@lists.utsouthwestern.edu
Hello, I may not have read every e-mail on this discussion but would like to
chime in here. I was just having this discussion with someone yesterday. At
large research hospitals that have various goals in our mission statements, we
are concerned about continuously improving the process to
Hello, we are using the labels from the Brady label maker with good success.
They are extra sticky and you can find some that will stick to paraffin. We use
ones that qualify for freezing since they need to be safe on the ice tray.
http://www.bradycorp.com/
Helen
-Original Message-
Hello, We are storing our unstained slides at -20 in Ziploc bags. Our TMA
blocks are being stored at 4 degrees.
Helen L. Fedor
Oncology Tissue Services, Manager
Johns Hopkins University
600 N. Wolfe St, | Marburg Room 406
Baltimore, MD | 21287-7065
410.614.1660
http://tmalab.jhmi.edu/
We have been using store bought gallons of distilled water in our water baths.
This water has been boiled so enzyme activity should be absent.
Helen
410.614.1660
http://tmalab.jhmi.edu/
http://prostatebiorepository.org/
Helen
-Original Message-
From:
You will also need to validate all of the IHC stains and special stains that
you do on these tissues. You can take samples from each tissue type and run
them in parallel on both machines. Then test all of your special stains and
antibodies that you have in your inventory.
:)
Helen
Agreed!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Monday, November 19, 2012 11:16 AM
To: Rob Day; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Need better results
Hello, We have a Bliss imaging system that we are no longer using. I has a
great Zeiss axiophot microscope with high quality lenses. I believe that
Olympus is now the company that has taken over the Bacus Labs Imaging system.
Please contact me if you have a desire to acquire this system from us
http://www.pathologydevices.com/TMArrayer.htm
You can contact Ron Gebing at Pathology Devices.
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice
Frederick
Sent: Wednesday, July 11, 2012
That is so true.!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Pierce
Sent: Tuesday, July 03, 2012 1:36 PM
To: Jay Lundgren; Victor A. Tobias
Cc: HISTONET
Subject: Re: [Histonet] Billing 88342
Hello Colleen, We use standard size molds (2.5cm X 3.5cm) that are a litte
deeper (7mm) in our core to make the recipient blocks. 2.5cm X 3.5cm. and our
TMA with the 1.0mm punch have 13 rows and 16 columns = 208 cores. You can
contact me if you want more information.
Helen L. Fedor
Tissue
Hello, We have a position available for a casual temp employee. This is a
reference lab that primarily focuses on animal specimens. We will consider
someone who has 2 years or more cutting experience. Please forward your resume
to me.
thanks
Helen
410.614.1660
http://tmalab.jhmi.edu/
://tmalab.jhmi.edu/
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Helen Fedor
Sent: Monday, January 09, 2012 1:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Casual Temp Position
Hello Terry,
These are very difficult. The colder the better. (-27). keep the slides in the
cryostat. Cut the section. Place section on a cold slide, and keep the slide in
the croystat during the following procedure.
Hold slide in left hand and brush in right. Place finger under one edge of
When the blocks are not croyprotected you can cut them at warmer temperatures.
But when they are cryoprotected with the 30% sucrose, they are soft at -18,
that is why you need the colder temperature. they will compress at the warmer
temperatures and give you a lot of wrinkles.
Helen
Hello, Do 30 minutes at 37 degrees on a glass slide. take from oven and apply
gentle pressure to center of block and slide complex, cool to RT and repeat 1
or two times.
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
handling the slides .(including
the process of labeling slides). I usually lay the slides out on pape rtowels
on the lab bench while labeling them.
Helen Fedor
Johns Hopkins University
TMA and Prostate Spore Lab Manager
JHU SOM Uro/Pathology
600 N. Wolfe Street, Marburg Room 406
Baltimore MD
Hello, We have been storing our slides in small zip loc bags at -20 and have
data suggesting that slides stored in this fashion are reasonably well
preserved after 3- 5 years. This was done with TMA slides. Was not done for
special stain slides but for IHC.
Helen
-Original Message-
Hello, There is a company that I just ran a few months ago.
http://www.pathologydevices.com/TMArrayer.htm they have a manual arrayer that
is running about 30,000.00. A little more than the Beecher Arrayer but it does
offer some improvements. They are also able to sell the Needles that will fit
-7065
410.614.1660
From: Mark Tarango [mailto:marktara...@gmail.com]
Sent: Friday, March 04, 2011 4:20 PM
To: Helen Fedor
Cc: Barone, Carol; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Beecher Instruments
There is also the Sakuro Quick-Ray System. I don't know if you've
Since automation is becoming more and more a part of all Histology labs the
demands of placement of the tissue on the slides varies for different
instruments. Stainers, coverslippers and now with slide scanning as well. So I
do not believe that there is a silver bullet answer.
Helen L. Fedor
Hi, A question has come up regarding the different methods used to put a charge
on the slide. We recently ordered some plus slides and the boxes they are
packed in say silanized slides, but they say plus on the slide . We don't
want to use these for our clients if they are not going to be
[mailto:irena.kir...@hotmail.com]
Sent: Wednesday, December 22, 2010 1:54 AM
To: Helen Fedor; sette...@umdnj.edu; ahut...@dh.org;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Edge affect on TMA?
how to prevent spots coming off during sectioning TM's? we do sometimes facing
problems sectioning
Hello, We do not usually see that. If we do its very rare because I can't think
of any examples off hand. The usual problems are the spots came off or
shifted/smushed, not an edge effect.
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
http://www.amazon.com/Surgical-Pathology-Dissection-Illustrated-ebook/dp/B000PY3QPM
here is the one that we use.
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tracz...@aol.com
Sent: Wednesday,
The study that we did showed that the staining on freshly cut slides from a
block stored at room temperature was in fact not as good as slides that were
sectioned 5 years earlier and stored at -20. Therefore the blocks should be
stored in the cold as well. The tissue is degrading in the block
Hello, We have not published the paper yet . It is in progress and hope to get
it out of the pile soon.
Helen
From: dusko trajkovic [mailto:dunat...@sbcglobal.net]
Sent: Thursday, November 04, 2010 1:45 PM
To: Morken, Tim; Helen Fedor
Cc: histonet@lists.utsouthwestern.edu
Subject: Re
Hello, We have been storing our slides in very small Ziploc bags at -20 and
find that this method works fairly well. We have done a study(Berez et.al in
process) and slides stored in this fashion for 5 years stain better than
freshly cut slides from the same blocks that have been stored at room
Hello, Our institution does release blocks, the requirement is that a patient
consent is signed and we have an MTA (Material Transfer Agreement) For the
study and appropriate IRB's in place for the study. It is a lot to set up, but
after this is done for one study any following studies are
Hello, We have trouble with the larger size punches as well. In order to get
the cores to stick to the size try warming the block in a oven at 37 degrees
for 15 minutes, put the block face down on a clean slide. (providing the
melting temperature of the paraffin is about 57 degrees). After 15
Hello, We have trouble with the larger size punches as well. In order to get
the cores to stick to the size try warming the block in a oven at 37 degrees
for 15 minutes, put the block face down on a clean slide. (providing the
melting temperature of the paraffin is about 57 degrees). After 15
You can try disecting trays. I just googled discting tray, looks like listing
price is 17.00. they are the perfect size and shape for freezing. and keep
blocks organized.
Helen Fedor
Johns Hopkins University
TMA and Prostate Spore Lab Manager
JHU SOM Uro/Pathology
600 N. Wolfe Street, Marburg
Hello, We have done a study and found that storage in small zip-loc bags at -20
is an inexpensive and convenient way to store slides. In fact the slides stored
for 5 years at -20 stain better than freshly cut sections from the same blocks.
This was also published recently by others. Dr Rimm at
Hello, Our lab does IHC staining in house and have had zero problems with our
Fisher brand cover slips until recently. We've tried both Fisher and Corning
cover slips and what appears to be dust or imperfections in the glass are found
on both brands. This is creating false positives for us,
I also receive one of the scam emails.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Thursday, November 12, 2009 9:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]
To subscribe or unsubscribe to the list or change your subscription to the
daily digest mode etc. you need to go to:
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or email:
histonet-reque...@lists.utsouthwestern.edu with the word help (without
quotation marks) in the subject line
Hello, We have a project that has worked on this topic using TMA's and have
found that storing unstained, and unbaked slides at -20 enhances the
preservation of antigenicity of the samples. It is not yet published but is
getting written up. TMA slides that were stored for 5 years at -20 in very
I believe that Brain Research Labs has large + slides.
Helen
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth
Sent: Thursday, March 05, 2009 10:07 AM
To: histonet@lists.utsouthwestern.edu
Hello, Another question, Is anyone using the Pathos system to process animal
tissues? I would really appreciate any comments good/ and or bad on this topic.
Best regards,
Helen
From: Helen Fedor
Sent: Wednesday, February 18, 2009 5:33 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Pathos
Hello, Our Surgical Pathology Department has been using the Pathos processor. I
work in research and we have begun to have issues with the tissue falling off
of the slide after pretreatment for IHC. Has anyone else noticed this
happening? The tissue is much harder in the block than the standard
Hello, I had previously sent this but only to the person who asked the
question, I think it this works beautifully so decided to resend to the list.
we have a great way to remove the specimens for the chucks. We have a 500cc
plastic container with a lid that we have cut an X into the center.
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