Hi Claudia,
We use Dako anti-Human Tau (rabbit polyclonal) at a concentration of 1:8000. We
do not use a pretreatment (HIER or protease). Staining is done on a Ventana
stainer.
Good luck,
Willem
Van: histonet-boun...@lists.utsouthwestern.edu
I am not sure if you are talking about the Beta Amyloid antibody, but if you
are: the best way to do the antigen retrieval is 3 minutes in formic acid.
Willem
Van: histonet-boun...@lists.utsouthwestern.edu namens Pirici Daniel
Verzonden: di 18-2-2014 23:59
Hi,
SMAD4 is very difficult on the Ventana platform. The only clone that would work
on the Ventana to my knowledge(clone EP618Y, immunologic) has recently
'collapsed' (i hope that's the right word?) It means that it is not available
anymore. Maybe there is some vendor that has a few vials
is: http://www.histosearch.com/rene.html
From: Rene J Buesa rjbu...@yahoo.com
To: Hoekert, W.E.J. w.e.j.hoek...@olvg.nl; vtol...@cox.net
vtol...@cox.net; histonet@lists.utsouthwestern.edu
[]
Willem, yes, there are. Look through the attached references which include
some for breast fixation
I was wondering,is there any literature on this subject? i.e. the minimal
required fixation time of breast tissue in order to get reliable immuno
staining (Oestro, Prog and Her2neu and ISH). We immunologists are alway trying
to convince our pathologists about the importance of good fixation,
Hi Kenia
I guess you will need at least two blocks because they are never 'positive' all
four of them in the same tumour. You might search your archive for young people
with colon cancer. There is a good chance that you will find positive material.
Willem Hoekert
OLVG
The Netherlands
Hello Eva,
We are not using pronase anymore, but when we did, we were doing it at room
temperature. (15 mg in 250 ml PBS, sigma P-5147). It worked fine.
Our Trypsin antigen retrieval, we were doing it at 37C as follows: the evening
before the retrieval, we put some PBS in the 37C stove, the
Hi Histonetters,
Does anybody has experience in making your own HP control tissue? I am tired of
using positive biopsies of patients since they are always almost finished. I
have heard of a procedure on making HP controls but I am not exactly sure how
it is done.
I have tried the
Hi Histonetters,
I am having a very hard time in setting up the MDM-2 antibody on our Ventana
Benchmark XT's. I am using clone SMP14 from Becton Dickinson, which was working
fine on the Labvision. I have tried everything (even incubating overnight at
4ºC) and I am out of idea's. Maybe I should
Which stainer are you using? Because some clones will not work on a Venatana
stainer.
Willem
Van: histonet-boun...@lists.utsouthwestern.edu namens Chakib Boussahmain
Verzonden: ma 23-4-2012 4:20
Aan: histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet]
We are not using negative controls. We only add positive controls at the bottom
of our patient slides. Sometimes there are 3 different types of control tissue
(a TMA), so that you can use the same control block for several antibodies. You
could add a negative control in the same block as the
For the immunologic pAB cocktail we are using Protease 1 (4 min) and ab
incubation of 32 min. The only problem is that mast cells are also staining, it
is a side effect of the protease 1.
(AB concentration 1:400)
Willem Hoekert
OLVG Amsterdam
The Netherlands
Hi Sandra,
I am using Neomarkers (Glut 1 Ab-1, polyclonal) on the Benchmark XT.
30' cc1
32' ab incubation
1:50
It works fine.
Willem Hoekert
OLVG Amsterdam
The Netherlands
Van: histonet-boun...@lists.utsouthwestern.edu namens Diaz, Sandra
slides.
So from now on, if we have a case that washes off, we will be using the Dako
slides.
Thanks for your help,
Willem Hoekert
OLVG AMsterdam
The Netherlands
Van: histonet-boun...@lists.utsouthwestern.edu namens Hoekert, W.E.J.
Verzonden: do 20-10-2011
Hi Histonetters,
Every once in a while, we have mamma tissue that washes off the slides
completely. The reason for this is that the tissue is still too fat and/or not
sufficiently fixed. There is no problem with the HE stain, but the immuno will
wash off. What do you do in such cases?
I have
Hi Phyllis,
We are doing a double stain (Mib-1 - P16) on cervix biopsies in which the P16
antibody is coloured with AP. It works fine, our pathologists are quite fond of
it. Almost every day we have a few slides.
Willem Hoekert
OLVG, Amsterdam
The Netherlands
We are currently running the Ventana IHC stainers with the UltraView Detection.
Just like us!
MLH-1: Cell Marque (ref 760-4264). 30' cc1, 32' ab incubation.
MSH-2: Cell Marque (ref 760-4265). 60' cc1, 60' ab inc.
PMS2: Becton Dickinson, clone A16-4, cat nr 556415. 30' cc1, 32' ab inc.
Thomas Jasper
Verzonden: do 17-3-2011 18:32
Aan: Hoekert, W.E.J.
CC: histonet@lists.utsouthwestern.edu
Onderwerp: RE: [Histonet] RE: Procedure for making Gram Control
Some sort of small, snack sausage of questionable quality. I'm not familiar
with anything like that in Europe, but maybe you could
What would be the equivalent of a Slim Jim in Europe? The Netherlands to be
more precise?
Van: histonet-boun...@lists.utsouthwestern.edu namens Walter Benton
Verzonden: wo 16-3-2011 15:07
Aan: Hayes, Randi (HorizonNB); histonet@lists.utsouthwestern.edu
Yes, we see the same. We also see staining in follicles in appendix and colon.
Van: histonet-boun...@lists.utsouthwestern.edu namens Andrea
Verzonden: do 9-12-2010 15:05
Aan: Histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet] ER staining in Tonsil.
Good
Not here in The Netherlands.
Van: histonet-boun...@lists.utsouthwestern.edu namens Laurie Colbert
Verzonden: vr 12/11/2010 15:42
Aan: godsgal...@aol.com; Histonet@lists.utsouthwestern.edu
Onderwerp: RE: [Histonet] Formalin
Not us - in Pasadena CA.
We used the same antibody but then at 1:1200, retrieved with PH9 (PT module
buffer 4, thermo) on a labvision stainer. We had beautiful results.
Now, we switched to Ventana Benchmark XT (1:50, 30' cc1 + amplification) and
our results are quite poor for PMS2: many cytoplasmatic staining.
So
Hi Histonetters,
We are thinking of taking the Ventana Benchmark XT, and I am testing it right
now. So far, it is impossible to get the Smad4 up and running. I have tried
everything:
-Pretreatment with cc1 and cc2 for up to 90 minutes
-Incubation of primairy AB for up to 90 minutes
Dear histonetters,
I was wondering if it is possible to do an EBER ISH on a cell-smear. What
pretreatment should I use?
1: Fix the cells in formalin (let's say overnight, or else one or two hours),
and than use the proteinase K digestion step.
2: Or maybe it is enough to fix the cells in
Are you sure that you don't introduce air bubbles when you put your slides into
the coverplates? The antibody will not touch the tissue if there is an air
bubble.
Willem Hoekert
Van: histonet-boun...@lists.utsouthwestern.edu namens Rene J Buesa
Verzonden: do
We are using Spot Light her2 Cish from invitrogen (manual, FDA approved).
Willem Hoekert
Pathology
OLVG, Netherlands
Van: histonet-boun...@lists.utsouthwestern.edu namens Taylor, Jean
Verzonden: di 5-1-2010 22:02
Aan: 'histonet@lists.utsouthwestern.edu';
* Cyclin D1 : NeoMarkers, SP4
* CD56 : NeoMarkers, 123C3.D5
* CD138 : Dako, M115
* Kappa : Dako, A0191 (Polyclonal)
* Lambda : Dako, A0193 (Polyclonal)
* MUM-1 : Dako, MUM1p
Willem Hoekert
Pathology, OLVG
The Netherlands
Disclaimer:
Dit e-mail bericht is
contains tween already. And yes, we do get good support, but still the problems
are not solved.
Thanks again,
Willem Hoekert
Pathology Lab, OLVG
Amsterdam
The Netherlands
Van: histonet-boun...@lists.utsouthwestern.edu namens Hoekert, W.E.J.
Verzonden: vr 9
Dear Histonetters,
A few months ago, we bought 2 Labvision Autostainers 720 from Thermo
(immunostainers). We have some bad experiences with them.
They seem to skip some slides every now and than (it happens maybe once every
3-4 weeks, we stain about 150 - 200 slides a day). If so, our
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