Re: [Histonet] IHC validations

2020-06-02 Thread Ingles Claire via Histonet
Paula: Usually CLIA follows CAP's lead or has less strict guidelines. Following the CAP guidelines should be good for both. Claire From: Paula via Histonet Sent: Tuesday, June 2, 2020 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC validati

Re: [Histonet] UV Light in Fume Hood

2020-05-14 Thread Ingles Claire via Histonet
UV-C wavelength is the best for the virus killing. Claire From: Paula via Histonet Sent: Thursday, May 14, 2020 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] UV Light in Fume Hood WARNING: This email appears to have originated outside of the U

Re: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel

2019-12-12 Thread Ingles Claire via Histonet
OMG!!! really?!? OSHA, Joint Commission, CAP, ANY regulatory agency. Give them the lounge microwave and get a new one for your break room immediately!! Fumes, contamination on the outside of whatever it's in, etc. Not even mentioning if it gets spilled. But then again, our docs like to store Bot

Re: [Histonet] Flammable Sprays in Cryostats

2019-09-25 Thread Ingles Claire via Histonet
Liquid Nitrogen guns. Claire From: Knutson, Deanne via Histonet Sent: Wednesday, September 25, 2019 7:50 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Flammable Sprays in Cryostats WARNING: This email appears to have originated outside of the UW

Re: [Histonet] Xylene substitutes for clearing agents

2019-08-28 Thread Ingles Claire via Histonet
Chris: Propar from Anatech works great for us. I believe it is still advisable to use xylene in the cleaning cycle on the processors though. We had to go back the other way a bit when our Doc wanted a tape coverslipper. Now he gripes about the xylene smell. Hmmm. Claire

Re: [Histonet] Softening Calf Hooves

2019-03-21 Thread Ingles Claire via Histonet
Sandra: We here at the Dermatopathology department of UW Mad have found: -10% Potassium Hydroxide works best for us on our nails. -We usually only need to have it in solution for a few hours. As you are trying to soften hoof, I might play with both concentration and time in solution. - I don't b

[Histonet] ST4020 Stainer

2019-02-15 Thread Ingles Claire via Histonet
Netters! A question on behalf of a co-worker. We are in the process of validating the ST4020 stainer from Leica. We are a Mohs clinic that currently uses Carazzi's double strength Hematoxylin. We are looking for a better staining protocol. We are planning on moving to a Harris formula when s

Re: [Histonet] Processing cystic tissue

2017-04-04 Thread Ingles Claire via Histonet
Charles: I usually get out as much of the cystic material as I can without damaging the cyst wall itself. That is all the docs are looking at anyway. I am assuming you are referring to skin cysts, not ovaries, etc. Claire From: Charles Riley via Histonet

Re: [Histonet] Reference for this Recipe and Use

2017-03-27 Thread Ingles Claire via Histonet
Sadly, it is much safer than the old Chloroform clearing method. Claire From: Tony Henwood (SCHN) via Histonet [histonet@lists.utsouthwestern.edu] Sent: Sunday, March 26, 2017 6:22 PM To: ian bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histo

Re: [Histonet] Performing PAS with Diastase by Hand

2016-10-05 Thread Ingles Claire via Histonet
We get our Diastase powder from American Mastertech. Claire Dermatopathology lab University of Wisconsin Hospital and Clinics From: Vickroy, James via Histonet [histonet@lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 3:48 PM To: histonet@lists

Re: [Histonet] Histonet Digest, Vol 151, Issue 1

2016-06-01 Thread Ingles Claire via Histonet
Not to mention that if you inhale too many fumes you will pass out. One of the original anesthetics. :) Claire From: Douglas Gregg via Histonet [histonet@lists.utsouthwestern.edu] Sent: Wednesday, June 01, 2016 1:04 PM To: histonet@lists.utsouthwestern.edu

Re: [Histonet] PAS Stain

2016-05-05 Thread Ingles Claire via Histonet
I don't know, I believe Dr. Salk did the same with the polio vaccine. He even involved his family! Dedicated doctors... Claire From: Tony Henwood (SCHN) via Histonet [histonet@lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 6:33 PM To: Anne Murvosh

Re: [Histonet] PAS Stain

2016-05-05 Thread Ingles Claire via Histonet
The level of amylase in your saliva also depends on when you ate last. If you're spitting on slides after you just ate, the reaction will be weaker as the amylase will have been used up on lunch digestion. (also, you need to be careful about having your lunch salad contaminate the slide... :) Cl

Re: [Histonet] Running a histo lab without a histotech?

2015-10-23 Thread Ingles Claire via Histonet
Not to my knowledge. We have 3 derm/Mohs labs here in our system. There are only two of us who have our certifications. Heaven knows the world (or the lab for that matter) doesn't stop revolving when WE go on vacation. The third lab is a one person show and she isn't certified. Just so all the C

Re: [Histonet] are you desperate enough to hire a B.Sc. graduate?

2015-08-10 Thread Ingles Claire via Histonet
We have had this problem for years. We are more than willing to train someone. We are a small lab so a bigger concern is if they will fit in with the rest of us personality wise. Not that we just hire anyone willy-nilly, but many people with BS's have some lab experience/background anyway. We ju

Re: [Histonet] Manual Cassette Labelling

2015-08-06 Thread Ingles Claire via Histonet
We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # is 14-905-30 for black, 14-905-35 for red. We have never had a problem with them smearing or fading on the cassettes no matter what we subject them to. Claire From: Joanne Cla

Re: [Histonet] Marciafava-Bignami Disease

2015-08-04 Thread Ingles Claire via Histonet
I know I watch my exposure to Formalin and Xylene especially. I was diagnosed with NASH in 2007 (who knows how long before then, since I am asymptomatic) when I had a Pituitary Adenoma that had hemorraged (Boy talk about a headache!) Anyway, I have always wondered about the cause. I thought it w

[Histonet] RE: Paraffin-Plastic Stratification/Congo red problem

2015-01-23 Thread Ingles Claire
I believe amyloid does fade with age on cut slides. Frieda sited Bancroft and Cook that intensity decreases with age and "long-standing" deposits stain less intensely than smaller "Newly formed" deposits. She also lists a Crystal Violet method in the 3rd edition, but that it is more of a rapid s

[Histonet] Sakura slide racks

2015-01-21 Thread Ingles Claire
Histonetters!! I am looking for some replacement racks that hold 20 slides and that fit in the Sakura Tissue-Tek film coverslipper. For some reason our ordering department doesn't want us to/let us order directly from Sakura. Thanks Claire ___ Histo

[Histonet] Heme "boogers"

2015-01-15 Thread Ingles Claire
Hello All! We have been having an interesting problem with our Hematoxylin. It seems there are globules that form on the bottom of our hematoxylin wells in our automatic stainer (Leica XL). These globs are acellular, so it is not a fungus or something. We use a progressive Mayer's Hematoxylin

RE: [Histonet] Re: Flames at embedding centers

2014-11-19 Thread Ingles Claire
Old Histotechs never die. They're just well fixed... :). From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Mark Turner [mtur...@csilaboratories.com] Sent: Wednesday, November 19, 2014 1:53 PM To: Sand

RE: [Histonet] On the lighter side...

2014-08-11 Thread Ingles Claire
Old histologists never die, they are just well fixed... Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Michael Ann Jones [mjo...@metropath.com] Sent: Monday, August 11, 2014 9:07 AM To: Edwa

RE: [Histonet] On the lighter side...

2014-08-08 Thread Ingles Claire
Just a foot note to all this. I still can't get my certification listed on my work badge because they've never heard of it. I'll just have to give them a tutorial...*sigh* I think this type of stuff is what gets me burned out the easiest. The thing that saves me are my co-workers. Tim is right

RE: [Histonet] On the lighter side...

2014-08-07 Thread Ingles Claire
15 years and over 10 certified HTL. Claire Ingles From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Richard A. Carter [rcar...@cancer-test.com] Sent: Thursday, August 07, 2014 4:36 PM To: 'Jennifer M

RE: [Histonet] Basement Lab

2014-05-05 Thread Ingles Claire
Our main Histology lab at UW Madison Hospital is in the basement of the VA (They're) attached. We have to go UP a floor to get to the morgue. Although why they put the morgue on the main level is beyond me... Oh, wait. government administration... I am at one of the outlying clinics and at least

[Histonet] RE: Req Storage

2014-03-25 Thread Ingles Claire
I'm guessing the procedure you discribe does, as we have been doing it that way for years. We keep our hard copies on site for a week before sending them to be scanned. Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsout

[Histonet] OT: RE: wax on the floors

2013-12-11 Thread Ingles Claire
When you're finished with him, send Bruce my way!!! Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Blazek, Linda [lbla...@digestivespecialists.com] Sent: Wednesday, December 11, 2013 2:32 PM

[Histonet] RE: Yahoo link

2013-12-03 Thread Ingles Claire
Old Histologists never die, they're just well fixed... Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Morken, Timothy [timothy.mor...@ucsfmedctr.org] Sent: Tuesday, December 03, 2013 11:22 A

RE: [Histonet] slide and block discards

2013-02-18 Thread Ingles Claire
I'd be interested in it too. Claire From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Cheryl [tkngfl...@yahoo.com] Sent: Monday, February 18, 2013 12:25 PM To: histonet@lists.utsouthwestern.edu Subje

[Histonet] RE: Something different!

2013-02-11 Thread Ingles Claire
Seriously cool! Will have to try to read the whole thing in my spare time. The first few pages look intriguing already. Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Basis for Quality Work in a Histotech

2012-12-17 Thread Ingles Claire
You're right Tim. I don't know how much time I have saved by listening to my inner voice and doublechecking things when the case number and name on a recut/stain request. One of our docs is famous for putting down wrong case #'s. Even when everything looks OK, sometimes the docs accidentally mar

RE: [Histonet] RE: back up cryostat

2012-09-21 Thread Ingles Claire
I think one of the most important things to have a back-up for is a processor. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Manfre, Philip Sent: Fri 9/21/2012 7:15 AM To: barbara.cr...@lpnt.net; histonet@lists.utsouthwestern.edu Subject: [Hi

RE: [Histonet] RE: mouse testis in Bouins/Picric acid hazzards

2012-09-19 Thread Ingles Claire
Yes, but why take the chance. There are also other chemicals in the lab the picric acid can interact with to make it even more volitile than it was to begin with. Dynamite other explosives have the same problem. The older it gets the more degraded and unstable it becomes. One never knows if or w

RE: [Histonet] Unsubscribe

2012-07-24 Thread Ingles Claire
I vote for Raspberry! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of nmhi...@comcast.net Sent: Tue 7/24/2012 11:36 AM To: Stacey Crenshaw Cc: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Unsubscribe OH NO! They're BACK! Sain

RE: [Histonet] (no subject)

2012-07-23 Thread Ingles Claire
I'd love to know too. We also use a xylene substitute. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Brendal Finlay Sent: Mon 7/23/2012 12:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hello all! We are bud

RE: [Histonet] ER, Clone SP1

2012-07-16 Thread Ingles Claire
Don't give anyone any ideas!! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jonathan Cremer Sent: Fri 7/13/2012 1:45 AM To: Morken, Timothy; Kim Donadio; Patsy Ruegg Cc: Subject: RE: [Histonet] ER, Clone SP1 That drives me up the wall as

RE: [Histonet] Humidity Check

2012-07-03 Thread Ingles Claire
I think it is actually a requirement that temp and humidity are monitiored daily. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of lau...@blufrogpath.com Sent: Mon 7/2/2012 3:19 PM To: Histonet post Subject: [Histonet] Humidity Check D

RE: [Histonet] Mohs

2012-07-03 Thread Ingles Claire
I would love to know this too. I am getting about $23/hr but I have pretty good benefits. I'm at UWisc Madison Hospital. From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rebecca a. Johnson Sent: Mon 7/2/2012 5:37 PM To: histonet Subject: [Histonet] Mo

RE: [Histonet] Frozen Sections Falling Off Slides

2012-06-18 Thread Ingles Claire
Just remember - No formalin, no need for retrieval. In my experience that is the hardest step on tissue sections. It also shortens the procedure a bunch too. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Emily Sours Sent: Sun 6/17/2012 2:3

RE: [Histonet] Billing IHC on MOHS

2012-06-14 Thread Ingles Claire
Is this per "Specimen" or per surgical site. Mohs specimens are usually cut into smaller pieces and inked after the excision is removed. I would think this constitutes x# of blocks from the same specimen. Same as an excision that is breadloafed into separate sections. I don't think it matters wh

RE: [Histonet] ***News Flash*** OT

2012-06-12 Thread Ingles Claire
Not to TRY to offend anyone who actually has Leprosy, but "Wierd Al" Yankovic has a song titled "Party at the Leper Colony". The puns are really groaners. Almost makes me wish I had the disease so my ears would fall off... :) I'll find my copy (yes I am a big "Wierd Al" fan) if anyone is actuall

RE: [Histonet] Can use some help with processing references

2012-05-14 Thread Ingles Claire
We have also removed the blocks and let them harden until we are ready to embed them, then put back in the hot well on the embedder to warm back up and go from there. As they are already processed and infiltrated, I don't think there would be a problem leaving them at room temperature for a few

RE: [Histonet] RE: Qualifications for grossing

2012-04-25 Thread Ingles Claire
You can reprocess, recut, and restain, but never re-gross. I for one also gross, but only skin. And yes, I DO know how to gross an alopecia specimen. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Wed 4/25/2012 11:55 AM To:

[Histonet] While on the topic of harmful chemicals...

2012-02-23 Thread Ingles Claire
I have been having some health issues lately (understatement) Does anyone know the best formalin resistant disposable gloves? I gross (skin only) under a fume hood but the gloves still come in contact with formalin naturally. I am also (OK, mostly) interested in the health effects involving form

[Histonet] (no subject)

2012-02-14 Thread Ingles Claire
Hey histo gurus: Just a little twist for you. We are planning on doing GMS and PASD on frozen skin sections. Is there any change in staining protocol for paraffin vs frozen. We plan on fixing in ETOH. I have a co-worker saying that we don't need to use diastase on the PASD because there are alre

RE: [Histonet] anti static spray?

2012-02-10 Thread Ingles Claire
Are you sure it isn't just your electric personality? :) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kim Donadio Sent: Fri 2/10/2012 2:15 PM To: Elizabeth Chlipala Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] anti static

RE: [Histonet] interview cutting-OT-disarmingly long fordeletiondisinterested

2012-01-31 Thread Ingles Claire
Perhaps an issue that hasn't been discussed yet as to why there are so may "posers" out there for histology jobs is that dispite the fact that in relation to other lab areas we get paid less, in these economic times a job in a hospital - anywhere is great pay and often good BENEFITS i.e. health

RE: [Histonet] Stain and component shelf life

2011-12-13 Thread Ingles Claire
Can you share this info with me too? We just got grief about this from our JC inspector about a month ago. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/13/2011 8:04 AM To: histonet@lists.utsouthwestern.edu Subject

RE: [Histonet] Toluidine blue

2011-12-09 Thread Ingles Claire
Anyone know Dutch? From: histonet-boun...@lists.utsouthwestern.edu on behalf of Bruijntjes, J.P. (Joost) Sent: Fri 12/9/2011 4:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Henk Al weer even geleden: weet je al iets over de to

RE: [Histonet] Fibulin 3

2011-11-22 Thread Ingles Claire
Does this antibody detect little lies? Sorry, short week and am anticipating the turkey! Claire Subject: [Histonet] Fibulin 3 Does anyone have any experience with Fibulin 3 (EFEMP1) IHC in human tissues? Interested in opinions/observations on staining patterns and sub-cellular localizati

RE: [Histonet] RE: 15 years of Histonet

2011-11-15 Thread Ingles Claire
I didn't think space had a "side" anyway.?. You're not an egomaniac, You are just really proud of the work you do! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Mon 11/14/2011 4:36 PM To: Morken, Timothy; Histonet@lists.u

RE: [Histonet] Luciferase IHC

2011-11-02 Thread Ingles Claire
And it's not even Friday! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Kim Donadio Sent: Tue 11/1/2011 7:00 PM To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Luciferase IHC You have to soak it in h

RE: [Histonet] RE: Leica Bond verses the Ventana Ultra

2011-09-16 Thread Ingles Claire
James Bond? Sean Connery was always my favorite. :) Happy Friday! I'm off for a week and a half to the northwoods. Those fish better look out, and I don't mean zebrafish. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rathborne, Toni Sent: F

RE: [Histonet] Biocare decloaker

2011-07-27 Thread Ingles Claire
I swear I blow a gasket at least once a week! Oh wait that's me, not the decloker. :) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Weems, Joyce Sent: Mon 7/25/2011 3:42 PM To: William; Mike Pence Cc: ; Diana McCaig Subject: RE: [Histonet]

RE: [Histonet] How many tissues an histo tech is suppose to cut per

2011-06-28 Thread Ingles Claire
I frankly would like to be able to not worry so much about TAT so the larger tissues can fix better! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Grantham, Andrea L - (algranth) Sent: Tue 6/28/2011 10:05 AM To: undisclosed-recipients Cc

[Histonet] CE certification

2011-06-23 Thread Ingles Claire
Question to all you ASCP rule gurus - I got my certification before the stipulation involving having so many CE's in order to get recertified every 3 years. In order to be "current" with my certification, do I still need to get recertified every 3 years or am I grandfathered in to the old rules

RE: [Histonet] Retirement

2011-06-17 Thread Ingles Claire
But what about wildfires? :) Otherwise send me an application. I have been to New Mexico (mostly Abiquiu area) a few times and loved it. Not too sure about Alberquerque though. Too many episodes of Cops based there. Taos is nice enough. ;) Claire From: histone

RE: [Histonet] RE: Retirement

2011-06-15 Thread Ingles Claire
Try about 30 (years)! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Blazek, Linda Sent: Tue 6/14/2011 2:25 PM To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Retirement 7 years compared to 8 months is giganti

RE: [Histonet] IHC pos. & neg. control question

2011-05-20 Thread Ingles Claire
And the block in question has already been proven positive using THAT procedure and antibody during validation. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Thomas Jasper Sent: Thu 5/19/2011 2:39 PM To: pete.peder...@healthonecares.com; h

RE: [Histonet] RE: OT: April Fool's Nonsense

2011-03-29 Thread Ingles Claire
I get a bit creative with my joke slides and actually use old tissue to cut out shapes . Process, embedd, etc. and put them on slides and give to the doc. in the middle of his other cases. I have a pumpkin face on one with the slide labeled "O'Lantern, Jack" etc. I have also been known to use th

RE: [Histonet] expired abs

2011-03-10 Thread Ingles Claire
I think my "abs" expired long ago! Happy Friday Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Patsy Ruegg Sent: Thu 3/10/2011 8:28 PM To: 'ihcrg Group (E-mail)'; histonet@lists.utsouthwestern.edu Subject: [Histonet] expired abs Just wanted

RE: [Histonet] Wage question for Wisconsin

2011-02-15 Thread Ingles Claire
The answer to that question may have to wait a week or two. At least here at the UW. Our wonderful governor is trying to get rid of the state workers unions and collective bargaining. He is trying to push it through (it was just introduced this last Friday), and the vote is slated for later th

RE: [Histonet] Re: saliva for glycogen hydrolysis

2011-02-15 Thread Ingles Claire
Wouldn't GUM be a more palatable option? Who knows where some of those rubber bands have been! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Robert Richmond Sent: Mon 2/14/2011 11:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histone

RE: [Histonet] Help with OCT problem

2011-02-10 Thread Ingles Claire
Same with ETOH. We wipe our surfaces down with it to clean them so no cross contamination as we freeze our skin here on the metal side areas of our cryostats. If the alcohol hasn't been wiped dry or evaporated we have that problem too. Claire From: histonet-bou

RE: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI

2011-02-09 Thread Ingles Claire
ed and > would be happy to give you more details. > > Regards, > > Therese > Slone Partners > > On Feb 8, 2011, at 6:35 PM, Ingles Claire wrote: > >> Sorry Therese, but - >> Only consider this position if you like to be Really busy. As I > respect all on th

RE: [Histonet] Seeking a Surgical Pathology Supervisor in Madison, WI

2011-02-08 Thread Ingles Claire
Sorry Therese, but - Only consider this position if you like to be Really busy. As I respect all on this list. I will issue a warning that this position is much more involved than the 'ad' says. You would be overseeing the Histo lab, IHC lab, Renal biopsy lab, Grossing area, Dermatopathology la

RE: [Histonet] Re: Storing acetic acid ***

2011-01-25 Thread Ingles Claire
GEE, and all us young, wet behind the ears techs thank goodness you are here to keep us on the straight and narrow.(OK, maybe not the second part...) I learn so much from you guys. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of John Kiern

RE: [Histonet] History of Histology

2011-01-05 Thread Ingles Claire
This book, and a couple of others by Mitchell, are availible for free if you have a Nook (Barnes & Noble e-reader). You won't believe all the older (circa late 1800's, early 1900's) histo & pathology books. Nothing by Brian Bracegirdle though. There are plenty of interesting reads if time permit

RE: [Histonet] Hellerstrom & Hellman anyone?

2010-12-01 Thread Ingles Claire
Did you acid clean your glassware before hand? Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Amos Brooks Sent: Wed 12/1/2010 12:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hellerstrom & Hellman anyone? Hi, I jus

RE: [Histonet] High temp/paraffin oven

2010-12-01 Thread Ingles Claire
Not as much room/air flow for vents to dissipate extra heat? Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of histopa...@hotmail.com Sent: Wed 12/1/2010 4:19 PM To: Histonet Subject: [Histonet] High temp/paraffin oven Hello to all, Any

RE: [Histonet] Un related to actual histology

2010-10-18 Thread Ingles Claire
Ah---A woman after my own heart. I always thought of going as Bunson Honeydew or (especially) Beaker from the muppet show. I work in a clinic setting and have always thought of coming in as the nurse/ Daryl Hannah character from Kill Bill would be interesting, but I don't want to give the patie

RE: [Histonet] RE: Cutting, Processing, etc

2010-09-16 Thread Ingles Claire
DON'T get me started... And I have only been practicing 9 years! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Nails, Felton Sent: Thu 9/16/2010 11:06 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Cutting, Processing, etc

RE: [Histonet] Will a Thermo Scientific Sales Representative pleasecontact me

2010-09-08 Thread Ingles Claire
Maybe they're all pestering me. :) I believe Leica has Thermo now. I know they have closed at least one factory in my region, maybe more. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Blazek, Linda Sent: Wed 9/8/2010 3:40 PM To: 'Horn, Hazel

RE: [Histonet] Silver

2010-09-07 Thread Ingles Claire
I thought Histotechs were supposed to have purple thumbs. :) (You know, gardeners have green thumbs...) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of O'Donnell, Bill Sent: Tue 9/7/2010 4:15 PM To: histonet@lists.utsouthwestern.edu Subject: [

RE: [Histonet] caris and genoptix experience

2010-09-07 Thread Ingles Claire
Gee... It's not like WE have work to do or anything. Thanks for the heads up about these companies. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of kim.dona...@bhcpns.org Sent: Sat 9/4/2010 10:55 AM To: histot...@imagesbyhopper.com Cc: histo

RE: [Histonet] Corrective Action Form

2010-08-23 Thread Ingles Claire
Laurie: We just have a little form made out that we keep by the QA sheets for the stainer that lists date, problem encountered, and what the resolution was. That is for H&E's though. Immunos may be a bit more complicated. Claire From: histonet-boun...@lists.utso

RE: [Histonet] I Have been scammed

2010-08-18 Thread Ingles Claire
Glad you're not stuck, Jerry. This is why I act like an old codger and refuse to get a facebook,other social networking sites, etc. account. What's the deal with farmville anyway? :) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Jerry Santi

RE: [Histonet] Retaining empty jars

2010-06-23 Thread Ingles Claire
Ditto. Claire re: CAP retention guidelines say that wet tissues are to be retained for 2 weeks after final signout. We keep all containers, empty or not, for the 2 weeks then strain and discard them in biohazard trash. ___ Histonet mailing list

RE: [Histonet] Antibody Validation

2010-06-15 Thread Ingles Claire
Funny, I'd think the vendors would want you to do tons of your own validation, as it uses up antibody faster, ergo they get to sell you more antibody! :) In a feisty mood today. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa Sen

[Histonet] Sodium borate?

2010-05-20 Thread Ingles Claire
Help! Does anyone know a substitute for sodium borate in the GMS stain? Ours got unknowingly thrown out by our out-date police and he didn't tell anyone, and now we need it ASAP. And we don't have half of the stuff for the Warthin-Starry either. (Derm lab. We do this stuff oh so often, but now o

[Histonet] Paraffin

2010-05-18 Thread Ingles Claire
A question has come up recently about whether or not to completely turn off our embedding center every night or not. I was under the understanding that the constant solidifying and re-melting in the paraffin pot damages the structure of the paraffin and makes it more brittle, etc. Is my memory c

[Histonet] CD 133

2010-05-05 Thread Ingles Claire
Thought I'd try again... Anyone know where I can send some slides for a CD133? Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] CD133

2010-05-03 Thread Ingles Claire
Happy Monday! (grumble grumble) I need to know if there are any reference labs out there that do CD133 for stem cells. Our doc wants this done, but we can't find anyone who does it. Thanks Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.e

RE: [Histonet] ascp exam

2010-04-21 Thread Ingles Claire
Are you kidding!?! I have an AA in Histotechnology and still didn't pass my (HTL) written on the first round. I did pass my slide review though, thank you very much. :) I passed on the second try, which included 3 years of OJT and tons of studying after graduating school. (BTW, I also have a BS

RE: [Histonet] Toluidine blue

2010-04-21 Thread Ingles Claire
Newcomer Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of zodia...@comcast.net Sent: Wed 4/21/2010 5:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Toluidine blue Hello to all, I was wondering if anyone knows where to pu

RE: [Histonet] PA baby sitter

2010-03-26 Thread Ingles Claire
That's OK, our poor PA has to babysit the residents, so she needs all the help she can get!! Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Tue 3/23/2010 5:24 PM To: rick.garnh...@memorialhealthsystem.com; Jeffrey Silverman

RE: [Histonet] Re: bone marrow fixative

2010-03-03 Thread Ingles Claire
Ah, in a perfect world! (which would be down right boring, thanks) Claire Neutral buffered formalin requires time for fixation - clots need to be cut up as soon as possible after they're received, and biopsy specimens really ought to fix overnight before decalcification and processing. Communicat

RE: [Histonet] Alcohol Source?

2010-03-03 Thread Ingles Claire
I vote for the scotch! Claire depends. A fine Scotch whiskey may be aged in old oak casks, while a delicate Tequila may not be ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.e

[Histonet] NSH teleconference

2010-02-24 Thread Ingles Claire
Would the person responsible for sending out NSH teleconference material please contact me. I have not recieved the materials for today. Claire Ingles UW Hospital and Clinics Madison WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:

[Histonet] Mohs FYI

2010-02-09 Thread Ingles Claire
Hey gang: Here with some good news for once. I found out today that we WON our appeal to JCAHO over our tissue retaining time. They are giving Mohs labs an OK not to have to retain our tissue!! I guess it is 'processed' tissue instead of 'gross' tissue. Retaining it doesn't does not help patient

RE: [Histonet] cleaning cryostat

2010-02-04 Thread Ingles Claire
I work in a Mohs Lab. (high output frozen skin) We wipe out the cryostat with 95% ETOH and clear out the shavings every day we use our machines. We normally tear down and completely defrost our cryostats every other month or so, depending on humidity and frost build up. (and available time to ha

RE: [Histonet] Research Associate job opportunity (Danvers, MA)

2010-02-02 Thread Ingles Claire
Ian: Love doesn't pay the bills these days. ;) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Ian Montgomery Sent: Tue 2/2/2010 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Research Associate job opportunity (Danvers,

[Histonet] tissue retention...again

2010-01-27 Thread Ingles Claire
Hey there: I would like to take a quick poll of all techs doing Mohs sections out there. I would like to know what you do with your tissue after being frozen sectioned, how long you keep it, how you store it, etc. Thanks Claire ___ Histonet mailing lis

[Histonet] tissue retention times

2010-01-21 Thread Ingles Claire
OK, everybody crack open your JCAHO requirement manuals... We are a MOHS lab and were surveyed by Joint Commission about 2 months ago. During the survey, we were cited because we only retain our physical tissue specimens for 24 hours, as they remain fresh and are not fixed. We throw the tissue t

[Histonet] FW: FW: Follow Up: Joint Commission Lab Survey CorrectiveAction Plan West MOHS

2009-12-16 Thread Ingles Claire
OK Guys, here's another JCAHO/CLIA controversy. Has anyone (Mostly MOHS labs) been sited for this? Currently we only save tissue specimens 24 hours in case the Dr. decides to send some to path after the fact. (don't get me going on morphology quality). Does anyone have a clear definition of "gro

RE: [Histonet] In need of light bulbs

2009-11-25 Thread Ingles Claire
Yes! Heaven forbid we don't need any dim bulbs in the lab. :) Sorry couldn't resist. Happy Turkey day everyone. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of alan taylor Sent: Wed 11/25/2009 8:37 AM To: jstaruk; histonet@lists.utsouthweste

RE: [Histonet] OSHA shoe requirements for histology

2009-11-18 Thread Ingles Claire
No open toed shoes for one. Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of richard wells Sent: Wed 11/18/2009 10:36 AM To: histonet Subject: [Histonet] OSHA shoe requirements for histology It is my understanding that OSHA requires some sort

[Histonet] storage temps

2009-11-04 Thread Ingles Claire
Hey gang: Just a simple quiz. What are the temp. ranges for long term slide and (more importantly) paraffin block storage? Claire ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Laboratory Licensing

2009-11-04 Thread Ingles Claire
Haven't heard of that one yet. Just so the Dr. is licensed to read the slides. Wouldn't it essentially (geographically) be the same as a Dr. reading consult slides from another hospital? Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Pratt,

RE: [Histonet] Plants & windows in lab

2009-10-28 Thread Ingles Claire
I didn't think they had sun in Seattle. :) Claire From: histonet-boun...@lists.utsouthwestern.edu on behalf of Blazek, Linda Sent: Fri 10/23/2009 10:39 AM To: histonet Subject: RE: [Histonet] Plants & windows in lab Don't go to the web site. It has a picture

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