Re: [Histonet] toluidin blue

IMHO, Borax ain't that dangerous, my Granny put it in her laundry. However... Toluidine Blue is basic, it stains acidic tissue components. It will stain differently at different pHs. It gives increased metachromatic staining at pH 4. What pH buffer system are you looking for? Sodium phosphate,

Re: [Histonet] Kimwipes

NO!! We used to use phonebook pages cut in half, worked better than kimwipes, but phonebooks are hard to find these days. I've worked at labs where they used tri-fold paper towels. Wasteful, but they work. Check Grainger? Or Statlab? I know there are ot

Re: [Histonet] Tissue repository

Sorry Igor, but if you can't get informed consent, it's not legal or ethical to sell patients' surgical specimens. There are laws in all 50 states. The body or parts thereof have to be explicitly donated. If you have informed consent, you can sell them for a large profit, as lots of non-transplan

Re: [Histonet] Paraffin on floors

Forget the floor damage. You don't use xylene because it's a suspected carcinogen and it isomerizes to free benzene, a known carcinogen, when it evaporates. Xylene vapors are heavier than air and stay in your reserve lung volume and do The Bad Thing. NEVER use xylene for cleaning surfaces. Not

Re: [Histonet] Wax in hallways

Fill an industrial mop bucket with xylene and go to town! JK ;) Scrape it, but try doubling up your sticky mat placement. Not just doorways, but around your embedding center, a perimeter around your microtomy area, etc. Sticky mats are cheap, tech time is expensive. Make sure your histotechs ar

Re: [Histonet] NBF usage in the world

I would say that's a true statement. There are some labs that use formalin substitutes, but they are rare. I would guess 95% of labs use 10% NBF. Xylene substitute use is more common. Nobody uses B-5 anymore, as far as I know. Don't think you can still even buy the stuff. You could make your o

Re: [Histonet] Cancel subscription please

That's not how you unsubscribe. You have to stage a daring commando raid on Histonet headquarters and hack the mainframe. Good luck with the lasers. On Fri, Jul 12, 2024 at 11:10 AM Robyn L Vazquez via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello, > Please cancel my subscription

Re: [Histonet] HTL Certification

Make flashcards, all your esoteric fixatives with recipes, and all your special stains with reagents and what they stain for. Work them both sides till you can get 100% consistently. For example, so you can answer the questions "What is the oxidation step in a GMS?" as well as "Which of these s

Re: [Histonet] LDT's Regulatiions by the FDA

Seems to me they're going after immunos. To my recollection, there are only a very few kits that have FDA approval. I would think it's going to be more a manufacturer problem than a lab problem. I doubt they would go after the labs when they can go after Roche and Agilent (Ventana and Dako). Bu

Re: [Histonet] LDT's Regulatiions by the FDA

Can I ask you to provide an example of a laboratory developed test? So we're all on the same page. A test that you are not buying from a vendor, but just a totally in house protocol, reagents, etc? Using a vendor's test kit off label? Would a simple special stain like a PAS qualify as a LDT if you

Re: [Histonet] Agarose embedded tissue arrays embedded in paraffin block

Where did the agarose come from? On Fri, May 17, 2024 at 12:09 PM Otto, Christopher M via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > > Hello everyone! > > I'm having trouble sectioning tissue array blocks where the array is in > agarose embedded into a paraffin block. I've chilled

Re: [Histonet] De-mummification procedure?

The Mummy's major weakness is fire, a common weakness among the undead. Since mummies tend to be dry and coated with various oils and resins, the revenant tends to burn very well. Thus fire is the only way to destroy the Mummy forever (aka de-mummification). Sincerely, Jay A. Lundgren, M.S., HTL

Re: [Histonet] Inquiry on Tissue Softening for Microtomy

Pleasant is not a mission parameter. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Inquiry on Tissue Softening for Microtomy

I wouldn't say softening, I would say hydrating. Ammonia water accelerates hydration of FFPE blocks. Nobody knows how it works, it's a mystery. Or at least I've never heard a scientific explanation. Only pseudo-scientific mumbo jumbo like "facilitates the removal of paraffin" which is false. G

Re: [Histonet] Alcohol

Call StatLab, 1-800-442-3573 On Mon, Apr 29, 2024 at 6:49 PM Naira Margaryan via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello, > > What type of ALCOHOL ETHYL you’re using in your histology lab.? > > We are thinking to get 20-30-gal drum. > > Could you please help me with that? >

Re: [Histonet] Premaking slides

I mean, you're not going to hand the pathologist an unlabelled slide, obviously. This leaves you two options, label the slide before the tissue is on it, or afterward. Labelling the slide before is easier and quicker. I'm gonna label my slides *immediately* before cutting the frozen, or squishin

Re: [Histonet] Premaking slides

You mean for frozens? On Thu, Apr 25, 2024 at 12:44 PM Karen Schieberl CA-San Francisco via Histonet wrote: > Good Morning, > I am not sure if there is a CAP or Joint commission Policy on pre making > slides for intraoperative consultation. Can someone let me know if there > is one? I have lo

Re: [Histonet] Embedding

My lab uses trained lemurs, so I certainly hope not. Virus-free.www.avast.com

Re: [Histonet] Microcredentials for Histology

What Jennifer said. Just what kind of jumped-up osteopath college are you people running up there? Shocking. You think they're looking for "microcredentials" at M.D. Anderson or Brigham and Women's? You're not going to help the shortage or the field by lowering standards. Just kill a bunch of p

Re: [Histonet] Temp agency not paying what they owe me, help?

d Search Partners > AN MRINETWORK MEMBER > www.alliedsearchpartners.com > Direct (Call) Line: 386 339 0839 > > > > -Original Message- > From: Jay Lundgren via Histonet > Sent: Tuesday, October 3, 2023 7:24 PM > To: histonet > Subject: [Histonet] Temp agency

[Histonet] Temp agency not paying what they owe me, help?

I've recently had the experience of a temp agency not wanting to pay my travel pay, after the assignment is over. First time this has ever happened. Does anyone have any advice? Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mail

Re: [Histonet] tissue in alcohol too long now brittle

Soak in Oil of Cedar. Sincerely, A histotech from 1901 On Thu, Jun 29, 2023 at 9:00 AM Paula via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello, > > This is a question for a colleague of mine. Her routine tissue samples were > left in 95% alcohol for 2 hours before she had a chanc

Re: [Histonet] (no subject)

IIRC, yes, I seem to remember at AFIP our NCOIC cursing and reprogramming Roberta every Friday, or for a three day weekend. Roberta was the VIP's name. That lovely science-fictiony tan and burnt orange color scheme. I wonder what happened to her? Maybe you bought her? They never would have let

Re: [Histonet] VWR(R) Q path(R) Safesolv, Solvent Substitute

Ay perdón, no conocía que ya existe el medio de montaje Q Path® Safemount 00647520 compatible con el sustituto. Perfecto. Atentamente, Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Jun 15, 2023 at 12:07 PM Jay Lundgren wrote: > I've used all of them and they work fine. There's two flavors, o

Re: [Histonet] VWR(R) Q path(R) Safesolv, Solvent Substitute

I've used all of them and they work fine. There's two flavors, one that smells like bug spray (aliphatic hydrocarbons based) and the citrus kind. If you have one of those people in your lab that freaks out about fragrances (which is an actual, recognized psychosis, btw, it's called hyperosmia and

Re: [Histonet] Coffee at the desk

Next thing you're going to be telling me it's not OK to embed with a 1 foot tall bunsen burner open flame and a Coke on the cold tray! On Fri, Jun 2, 2023 at 7:46 AM Piche, Jessica via Histonet < histonet@lists.utsouthwestern.edu> wrote: > It also doesn't set a good impression for everyone else i

Re: [Histonet] Get the Short Term or Long Term Lab Staffing Coverage You Need

As far as I know, Histonet doesn't really allow advertising for individuals or agencies *looking* for jobs. There are a very few, selective, agencies that are allowed to post on Histonet tastefully and infrequently, but they are buying, not selling. Or histotechs who let others know about open p

Re: [Histonet] What they don't want you to know.

I came out of the US military and you NEVER turn anything off. Well, the cold plates on the embedding centers, and you empty and clean your water baths, but other than that, you don't even turn the lights off. If, one time, a tech comes from the OR with a specimen at 3 AM, and knocks the 5 headed

Re: [Histonet] Sections sticking to tweezers

Forceps have to be cool and dry. You can cool them in the ice bath, but you have to dry them off. If the tiniest bit wet, they will stick to your ribbon.. Not just forceps, everything has to be cool and dry. Your blade, your microtome stage/ blade holder, your forceps, your slides, if any of t

Re: [Histonet] Blade holder assitance

Good thinking. On Fri, Apr 7, 2023 at 12:20 PM Ken M via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Charles: > > Is there a spacer under the blade to convert hi to lo profile? Is there a > spot for one? You may be missing, or have the wrong spacer. > > Ken >

[Histonet] Who sells that block deparaffinizer dohickey that everyone has in their lab.

Who sells that block deparaffinizer doohickey that everyone has in their lab. What brand is it? Leica? I don't have one in front of me right now. Thanks, Jay A. Lundgren, M.S., HTL (ASCP) Histotechs are the foot soldiers in the battle against Cancer.

Re: [Histonet] Clarapath SectionStar

If it does come out, it'll probably cost $9,000,000.00 anyway. A lot of places won't be able to afford it. Anyway, research scientists are making incredible advances in diagnosing cancer with circulating cancer dna blood testing. They can even tell if the tumor is primary or metastatic through a

Re: [Histonet] Clarapath SectionStar

>From their website, hilarious: "Long overdue for a change, the current process is entirely manual, non-scalable, and is dependent on a shrinking workforce. The COVID-19 pandemic has exacerbated labor shortages, creating unacceptable delays in the delivery of timely biopsy results and driving up h

Re: [Histonet] Molds- cold vs warm

Whoever is telling you to use cold molds needs to go back to clown college. That is totally, 100%, absolutely, wrong. There is some debate as to embed "wet" (cassettes submerged in paraffin bath) or "dry", and I will accept either, as mostly a matter of personal preference. BUT, in both of these

Re: [Histonet] best paraffin

Paraplast plus is my personal favorite. On March 10, 2022, at 11:04 AM, John Garratt via Histonet wrote: The paraffins available from lab suppliers (not candle makers) is all of excellent quality these days. There is some variability, but choice of paraffin usually comes down to personal pref

Re: [Histonet] Tiny folds in colon bx

If you're not, use actual ice to cool and hydrate, works better than cold plate, etc. Cool and soak the heck out of your bxs, like, rough cut them and go to breakfast. Seriously, like 30 minutes on ice, and they'll cut like butter. Try raising the temperature of your water bath 2-3 degrees. Als

Re: [Histonet] paraffin on floors

These work pretty well to keep wax from being spread around too much. https://www.grainger.com/product/PLASTICOVER-Floor-Protection-Mats-45MT85 Place them strategically in thresholds and high traffic areas. Unlike the blue semi-permanent ones you mentioned, which break down, when these get dirt

Re: [Histonet] 5 Yr old Leica ST5020 is down

> > Never buy an instrument without an extended service contract. > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ___ Histonet ma

Re: [Histonet] Mohs Tech Wage?

Depends on the qualifications you're looking for. If it's just a B.S., or less, then you're competing with Wal-Mart and Burger King and everyone else. The market sets the rate at about $15-$20 bucks an hour, as far as I know, outside of top 10 cities and West coast, right now. Just because you'r

Re: [Histonet] release of body parts

It's all fun and games until someone finds a uterus in a landfill. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Prussian Blue Reaction

Supposed to be insoluble. Try potassium permanganate followed by oxalaic acid. But book says insoluble. On Monday, June 7, 2021, Mac Donald, Jennifer via Histonet < histonet@lists.utsouthwestern.edu> wrote: > The instrument malfunction and it was overstained. > > From: John Kiernan > Sent: Mon

Re: [Histonet] Job Opportunities USA Jobs

Just curious, do you have to have a Florida license to work for the VA in Florida? Do you know the GS rating of the job? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Filed slides sticking together

I've seen labs that do this, like 30C for 24 hours, but if you use xylene, make sure to put the oven under a hood. It's a lot easier just to leave them out, in a well ventilated place, for 48hours. Buy more slide folders/trays. I worked one place where the slides were held for 24 hours before be

Re: [Histonet] Is there a way to orient FFPE sections with an indicator that remains visible post staining?

Those cassettes are for an auto-embedder and they are awful. Melt plain agar from micro in microwave, dye different colors with surgical ink, cool, cut cubes, embed with tissue. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.uts

Re: [Histonet] Fee based grossing histotech

I think it's more fair to pay them a share of the professional fee, based on the CPT code of the tissue being grossed. That's basically how a PA working for a group gets paid (although most are salaried), and you are asking a histotech to do a PA's job.

Re: [Histonet] Can't keep a good histo tech down

If you want to be a temp, be a temp. There are literally dozens of agencies you can join. Histonet is not a job board. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Is OKTo use blue cheese for fungus controls,

If it's stupid but it works, it ain't stupid. Sounds like a great idea. We used to use Slim Jims as a BH control at AFIP. So does that turn the blue cheese into processed cheese? :) On Fri, Nov 22, 2019 at 7:51 AM Akemi via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > Good morning

Re: [Histonet] Trouble shooting issues

"Poor staining quality" is a little vague. Could you please describe what you are seeing on the slide? On Tue, Nov 5, 2019 at 12:33 PM Charles Riley via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Can anyone let me know what possible causes for the poor staining quality > might be? >

Re: [Histonet] Histonet Digest, Vol 188, Glass plate for AO knife sharpener

How old is that thing? Reagan administration? They don't make 'em like that anymore. On Thu, Jul 11, 2019 at 3:13 PM Steve McClain via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Does anyone know where to find the glass plate for an ancient AO sharpener > for microtome? > > Steve > S

Re: [Histonet] Productivity Tracking

As I pointed out, management can incentivize this any way they want. You could give raises, or you could say a tech is not eligible for a raise until they can cut at a certain rate. The idea is to bring the slower cutters up to speed, not get rid of them. Based on my experience, if you isolate an

Re: [Histonet] Productivity Tracking

By the way, the standard for graduation from AFIP was 30 blocks/hr. Don't count blocks, count *slides*. If you have people slacking, you really need to start action to get rid of them. One rotten apple ruins the whole bunch. Google the new research on toxic personalities in the workplace. But

Re: [Histonet] Frozen Gel Pack with paraffin block for shipping?

That's awesome because the block is already chilled for you when you get it, so it saves time! JK, but I think it is necessary when you send a block to Texas in August. If you're going to have a protocol, it's best to just leave it that way all the time, even if you're sending a block to Buffal

Re: [Histonet] H&E Staining question

Obviously, you're going to get a much different result using a polychromatic stain, than by doing a standard H&E. But if the pathologists like it... I'm not even sure if they could call that stain an H&E in their dictation. Seems like that would open them up to liability if, God forbid, they ever

Re: [Histonet] Histotechs abroad?

I understand there is a shortage of histotechs in Syria, also eastern Ukraine. Have fun! On Fri, Oct 26, 2018 at 1:25 PM Wellen, Terry :LLS Lab via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Does anyone know if it is possible to work overseas? (esp. Spain) > What is the drill? > > Te

histonet@lists.utsouthwestern.edu

Just a guess, but maybe increased time in hematoxylin? :) On Thu, Sep 20, 2018 at 10:58 AM Charles Riley via Histonet < histonet@lists.utsouthwestern.edu> wrote: > My pathologists are complaining that our cell blocks appear light on > hematoxylin. > > Does anyone use a different protocol for the

Re: [Histonet] Ethanol in tissue processing

Most places use "reagent alcohol" which, from most suppliers, is a mongrel blend of whatever is cheapest, and not even listed on the label. It's usually made up of a blend of ethanol, methanol, and isopropyl. Good luck finding what the percentages are. I've called one vendor before, and even they

Re: [Histonet] Special stain questions

Any of them? If you mean what stain looks pretty, I would say Trichrome on umbilical, and uvula, hmm, I dunno. On Tue, May 8, 2018 at 1:05 PM, Charles Riley via Histonet < histonet@lists.utsouthwestern.edu> wrote: > What special stains can be done on normal uvular tissue and umbilical cord? > >

Re: [Histonet] Leica

Sad, the last Leica microtome I bought was packed in German newspapers. So much for the hundreds of years of tradition of precision German instrumentation and optics. On Tue, May 1, 2018 at 12:24 PM, Colleen Forster via Histonet < histonet@lists.utsouthwestern.edu> wrote: > 😕 > > On Tue, May 1,

Re: [Histonet] Suggested Requirements for new uncertified HTs

This is why we used to have a practical on our exam. Sigh... On Tue, Jan 9, 2018 at 5:19 AM, Erin McCarthy via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi All, > > I work at a Start-Up and I was our first hire. I have been practicing since > 2011 when I got my certification. Howeve

Re: [Histonet] Metal embedding molds-large

mold release Virus-free. www.avg.com <#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9F

Re: [Histonet] QIHC Study Materials

The free one from Dako. Virus-free. www.avg.com <#DAB4FAD8-2DD7-40BB-A1B

Re: [Histonet] Lab assistants

The most important role of the lab assistant in the Histology laboratory is to be the target of middle management ire. Sincerely, Jay A. Lundgren, M.S., HTL(ASCP) On Wed, Oct 11, 2017 at 4:00 AM, Hannen, Valerie via Histonet <

Re: [Histonet] Temp employees

Any of the temp agencies can provide a Mohs tech. On Tue, Sep 12, 2017 at 3:06 PM, Isabel Soto via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello... you can call Haleigh Sutherland at 1-801-930-3446 that is > CompHealth. Haleigh will take care of you. > Regards,Isabel Soto > > Sent

Re: [Histonet] Need help identifying a product!!!

It was a statlab thingy, I 'member that much. On Fri, Sep 1, 2017 at 1:41 PM, Paula Keene Pierce via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Have you tried MarketLab? They have things like that. Paula Keene Pierce, > BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake

Re: [Histonet] comparison between microtomes leica RM 2125RTS y thermo HM325

I prefer the Leica, because it has that nifty rough cut button, but that's just my personal choice. The Thermo has a better waste tray. I think you'd be happy with either one. The best thing for you to do would be to get your sales reps to bring them both to your lab to demo and pick your favori

Re: [Histonet] overseas jobs

It's not a matter of whether there is a shortage or not. Most western, developed countries, unlike the US, have strict laws forbidding non-citizens from working, which they zealously enforce. It is virtually impossible for a foreigner to work (legally) in the EU, for example. Canada has different

Re: [Histonet] Digital Pathology & Coverslipping

Well, film is the other choice. http://www.sakura.eu/Our-products/item/10/-Coverslipping/82/Tissue-Tek-Film-Coverslipper Agronomy Road, huh, gotta love the Aggies. Hullaballoo Caneck Caneck! Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Aug 11, 2017 at 2:42 PM, Alexis Templeton vi

Re: [Histonet] ClearRite

I've worked in labs using both Clearite and d-limonene, and both are fine for IHC and molecular studies. Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Jun 12, 2017 at 6:41 AM, Dessoye, Michael via Histonet < histonet@lists.utsouthwestern.edu> wrote: > We've used

Re: [Histonet] Looking for inverted microscope

Have you tried obtaining one from an Australian pathology lab? On Wed, Apr 12, 2017 at 3:03 PM, Cynthia Hutchinson via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Good afternoon, > I am looking for an inverted microscope (used, refurbs acceptable) for a > new investigator with limited

Re: [Histonet] I have a job

As far as I know, looking for a job is frowned upon, posting jobs is OK, once in awhile. This isn't Indeed.com On Mon, Mar 27, 2017 at 5:52 PM, Eileen Akemi Allison via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I thought we weren’t allowed to post jobs on histonet! I tried and it wa

Re: [Histonet] Recycled reagents in VIP processor

Yes, it was my suspicion that if you add in tech time , there is not a savings. The histotech is, after all, the most expensive piece of equipment you have in the lab. In my personal experience, most labs that start recycling don't keep it up, when they discover it doesn't really save them money.

Re: [Histonet] Recycled reagents in VIP processor

When people say they are saving money by recycling reagents I always wonder if they are including tech time (to run the still) in their calculations. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Feb 22, 2017 at 2:32 PM, Ma

Re: [Histonet] TGIF - Help me with my mental block! The "PA" dilemma...

pathologists' On Fri, Oct 21, 2016 at 6:56 AM, Pam Barker via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi Histonetters! > This might seem like a dumb question but every time I get a position for a > PA it seems that I can never remember if it should be pathologists' > assistant or p

Re: [Histonet] Microm HM 355S Issues

With most microtomes, 1 complete turn of the wheel equals one advance of four microns, or whatever you have the micrometer set on. What people don't realize with the HM355 is that is doesn't take a complete turn of the fine advance wheel to advance the specimen. If I recall correctly, the advance

Re: [Histonet] HT Certification Exam

Get a good night's sleep and eat a good, high protein breakfast! There's always a question about which fixative to use or not use with uric acid crystals/gout, so I just gave you a free one! Good Luck! Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Sep 14, 2016 at 7:56 AM, Van

[Histonet] Genomic Health?

Anyone from Genomic Health in Redwood City, Ca on here? Thanks, Jay A. Lundgren, M.S., HTL (ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://l

Re: [Histonet] On the hunt for new microtomes!

Leica On Mon, Aug 1, 2016 at 2:53 PM, Cooper, Brian via Histonet < histonet@lists.utsouthwestern.edu> wrote: > We've used Microm microtomes for years and they have always been 100% > trouble free. > > -Original Message- > From: Jennifer MacDonald via Histonet [histonet@lists.utsouthwester

Re: [Histonet] block scrapers

Buy cheap paring knives at the dollar store and blunt the edges? On Thu, Jul 21, 2016 at 1:46 PM, Lauren Sweeney via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi all, > > My lab is in need of some tools to scrap the paraffin off the edges of the > blocks after embedding. Does anyone

Re: [Histonet] HT Certification

Maybe someone can quote CLIA chapter and verse, but, in my understanding, only registered HTs or HTLs are supposed to be doing embedding, cutting, special stains and IHC unsupervised. I think the regulation says non-registered personnel (trainees) can perform these duties only under direct super

Re: [Histonet] Staining stomach cells

On H&E, parietal cells are eosinophillic , due to abundant mitochondria. Chief cells have basophillic cytoplasm on H&E, due to abundant rough endoplasmic reticulum. Other than that, I'd think you'd want to use IHC. Is here an antibody for intrinsic factor? Parietal cells secrete intrinsic factor,

Re: [Histonet] Tissue Controls (Deceptive)

Get out the pitchforks and torches! On Wed, Jun 15, 2016 at 11:26 AM, Sally Price via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Not cool Rachel. I took the survey assuming that it was intended and > might help a colleage, but it's nothing but a marketing ploy, which are > strongly d

Re: [Histonet] Static electricity Tip

They sell industrial anti-static sprays, you spray the equipment, the bottom of your shoes, and the floor. They work. http://www.amazon.com/ACL-Staticide-General-Purpose-Anti-Stat/dp/B00BR55KQ6/ref=zg_bs_3310249011_1 Also, you can ground your microtome to a pipe with a ground strap. On Wed, Jun

Re: [Histonet] floor vibration

Wouldn't the anti-vibration measures work better if placed under the laundry equipment? They sell super expensive air bearing tables for EM applications, but I doubt routine histology would merit such expense. Good luck, try to enjoy the good vibrations. On Mon, May 16, 2016 at 8:29 AM, Paula Ke

Re: [Histonet] Melanin Bleach

Cool trick Jeffrey! On Tue, May 10, 2016 at 12:14 PM, Maxim Peshkov via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Karen, > The bleaching reagents will not compatible before IHC anyway. > Some tips: > 1. Do IHC-test as usual and counterstain nuclei with methylene blue > Loeffler at 3-

Re: [Histonet] picric acid

https://www.youtube.com/watch?v=IqTZNn0UHdA Jay A. Lundgren, M.S., HTL (ASCP) On Thu, May 5, 2016 at 12:29 PM, STEVEN PINHEIRO via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Well you have the most important part correct- Keep it wet. But that > doesn't address how to dispose of it. Y

Re: [Histonet] Wrist Pain

I worked at a lab in Oregon that only let a tech embed for an hour at a time, before having someone else take over. They were very concerned about carpal tunnel prevention, obviously. They just did it informally, no schedule or anything. After an hour, you would stand up and say, "Who wants to e

Re: [Histonet] congo red - decolor

Decolorize Congo Red by soaking in a 1% alcoholic solution of Sodium Hydroxide. Heat and stirring might help, be careful not to soak your sections off the slides, NaOH is a strong base. Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Mar 17, 2016 at 1:13 PM, Nancy Schmitt wrote: > Hi

Re: [Histonet] congo red - decolor

Are you sure you don't mean Oil Red O stain for fat? Just checking. Congo Red is for amyloid. Destain Congo Red by soaking in 100% Propylene Glycol, warmth and stirring would speed it up. Jay A. Lundgren, M.S., HTL (ASCP) On Thu, Mar 17, 2016 at 12:41 PM, Nancy S

Re: [Histonet] Suggestions for making glutaraldehyde penetrate in between little setae of insects for microscopy

A little alcohol will lower the surface tension of aqueous solutions. Then there's always DMSO. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Fri, Feb 26, 2016 at 8:15 AM, Jorge A. Santiago-Blay via Histonet < histonet@lists.utsouthwestern.edu> wrot

Re: [Histonet] Davidson's fixative and IHC question

Today I learned what Davidson's fixative is! Thanks! Anyway, so it's a strong alcoholic formalin. The answer to your question is; yes. You need to be careful of overfixation, thus masking the epitope in question. From Dako: " Of the many pre-analytical variables which affect IHC and ISH

Re: [Histonet] formalin and shrinkage

I was taught at AFIP to expect shrinkage of 10%, in each dimension. So I guess that's 30% shrinkage overall? Shrinkage is partially caused by formalin crosslinking the proteins in fixation, and partially by dehydration. Maybe a little shrinkage in xylene too? From removal of fat in adipose tiss

[Histonet] Job in Chicago?

Anyone know whose lab is looking for a temp in Chicago? I've been contacted by a couple of temp agencies about an assignment in Chicago, but I've been enjoying working as an independent contractor for a while now. So if anyone is in Chicago, and your lab is the one looking for a temp, please fee

Re: [Histonet] unsolved proplem

What kind of processor are you using? On Tue, Jan 26, 2016 at 1:36 PM, Jennifer MacDonald via Histonet < histonet@lists.utsouthwestern.edu> wrote: > There are a couple of things that it might be. > 1. Uneven deparaffinization before staining. > 2. Water in your last reagents/paraffin on the pr

[Histonet] Running a histo lab without a histotech?

Hello histonetters! I am on assignment in a small hospital lab in the Heartland and they are staffed by only two people. One is a registered (ASCP) HT with 10 years of experience, and the other is a former phlebotomist being trained on the job from scratch , who has yet to take her accreditation

Re: [Histonet] what to do with a dead processor

IIRC, don't those paraffin reservoirs slide out? Maybe you could warm them up with a propane torch to get them out? Then put them in an oven or just use brute force and patience to chip the paraffin out? As to who would want it, I don't know? Is a replacement motherboard available? Seems like

Re: [Histonet] Freeze Spray Not Sold as Case

There's this cool thing called the Interwebz now? http://www.amazon.com/Multi-Purpose-Freeze-Spray-1-Ea/dp/B0017UIB30#Ask Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Aug 19, 2015 at 8:53 PM, ian bernard via Hist

Re: [Histonet] microtome blade

NEVER leave a blade in a microtome when not sectioning! Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Aug 19, 2015 at 9:12 AM, Zerfas, Patricia (NIH/OD/ORS) [E] via Histonet wrote: > Dear Histologists, > A