I had heard that CLIA was relaxing things and is not requiring a new # to
work from home right now. Best to check on the regulatory but FFPE isn't
typically infectious. The ideal spot would in the garage and not the
kitchen though.
On Thu, Apr 16, 2020 at 3:47 PM Roxana Robinson via Histonet <
While we're on the topic of NTRK, does anyone have a positive case that
they could share? I'm working up the FISH and have probes for NTRK1, 2 &
3. Any pan-NTRK positive IHC case would be great for detecting by FISH too.
thanks!
Mark Tarango
On Wed, Sep 11, 2019 at 11:23 AM Piche, Jes
slide
that had already been pretreated and hybridized. By doing this a few times
(even up to four tries), you will likely get FISH signals that you can see
and enumerate.
Good luck,
Mark Tarango
On Tue, Jun 11, 2019 at 11:33 AM Пешков Максим via Histonet <
histonet@lists.utsouthwestern.edu>
How about passing on to the department that could fix the issue? Where's
the empowering innovation?
On Fri, Mar 1, 2019 at 10:35 AM Jordan, Kelley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> More bad press in histonet...not sure if we should pass it on to
> Marketing??
>
> @Will,
I hope everyone is using on-slide controls :-)
On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Another one!!!
> Our Her2 antibody dispenser failed, LOT #E22628
> This one "supposedly" FDA approved.
> Roche, why do you continue to lie to
Would anyone have NTRK1, NTRK2, or NTRK3 positive tissues? We are trying
to validate break-apart FISH probes for these gene translocations but don't
have any positive tissue. If you have something positive by IHC, I would
be very happy to try FISHing it.
thank you
Mark Tarango
CellNetix
Hi Gudrun,
Are you sure you have digested long enough with pepsin? If the tissue is
not well digested you will see background. We use sodium thiocyanate for
pretreatment reagent, not citric buffer. These are my first thoughts.
Mark
On Tue, Feb 19, 2019 at 9:18 AM Gudrun Lang via Histonet <
The pathologist should be responsible for pathologic staging on excisional
specimens. The surgeon (sometimes oncologist) does the clinical staging.
At least that is what happens at our local tumor board meeting.
Mark
On Mon, Nov 12, 2018 at 9:05 AM Eck, Allison via Histonet <
consideration. Limiting the production of
unstained slides to small and scant needle may make storage more practical.
Just some more things to consider.
Sincerely,
Mark Tarango
On Thu, Aug 16, 2018 at 4:48 PM, P Sicurello via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
>
y the
type of fixative used and the cold ischemia time. In addition, any
treatment that may potentially
alter immunoreactivity, such as decalcification, must be included.
Mark Tarango
On Wed, Apr 19, 2017 at 12:29 PM, Jason McGough via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
&g
My lab does not have a written policy on this yet but are discussing it.
If the patient has a high grade tumor and HER2 IHC is 1+ (or even 0+), some
of our pathologists will reflex to FISH. The oncologists will request it
sometimes too, more often when ER and PR are both negative. We have found
You could try these from Ted Pella:
http://www.tedpella.com/glasswar_html/scriber.htm
On Wed, Mar 2, 2016 at 10:19 AM, Cartun, Richard via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Years ago, I obtained a metal scribe for etching glass slides, but I can't
> remember which Histology
Hello everyone,
I am working a validation for MET amplification by FISH and am having a
hard time finding positive cases. Would anyone have any to share or
trade? I have access to all kinds of cases from both IHC and molecular for
exchange.
thanks
*Mark Tarango*
Lead Molecular Technologist
I used to work in a lab that used compressed air held upside down as freeze
spray. Worked the same for me and might be cheaper.
On Wed, Aug 19, 2015 at 6:53 PM, ian bernard via Histonet
histonet@lists.utsouthwestern.edu wrote:
Fellow Histonetters: I'm looking for a source to purchase
It is April 1st, isn't it?
On Wed, Apr 1, 2015 at 4:12 PM, Paula Sicurello pat...@gmail.com wrote:
Good Afternoon Netters,
Since we went down the path of hot dogs and Slim Jim's as positive controls
for FFPE stains, I was wondering.
Is there a source, like a hot dog or piece of steak,
Did you ever run ALK IHC on lung cancer cases or have they always used FISH?
thanks
Mark Tarango
On Fri, Oct 24, 2014 at 12:34 PM, Joelle Weaver joellewea...@hotmail.com
wrote:
Recently all the pathologists I work with prefer the ALK FISH.
Joelle Weaver MAOM, HTL (ASCP) QIHC
Date
Does anyone stain lung cancer specimens for ALK using IHC? If not, any
opinions?
thanks
Mark
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My understanding was that this is just for Medicare patients...
On Mon, Jan 6, 2014 at 12:35 PM, Johns, Jill jjoh...@cpallab.com wrote:
The following was taken from the NCCI manual, Chapter 10, effective 1/1/14:
9. The unit of service for in situ hybridization reported as CPT codes
88365,
Hi Elizabeth,
Room temp is fine for FFPE tissue for DNA extraction. We do it this way
every day!
thanks
Mark
On Tue, Jul 30, 2013 at 12:02 PM, Elizabeth Cameron
elizabeth.came...@jax.org wrote:
We are preparing paraffin slides for DNA extraction, and I am not sure if
once cut they should
Hi Kris,
The polymer backbone for this detection kit is conjugated with anti-rabbit
antibodies only (AP Polymer reagent). The specific probe is a rabbit
anti-mouse antibody in the AP probe reagent. That is why you only need to
apply the AP probe when your primary antibody is from mouse but not
Yes, if the stain can wait get some mount quick from Newcomer. There is a
good chance the tissue will fall off if you proceed with the tissue on
regular slides. I would wait until I get the mount quick.
Everyone else reading this should order a tube now for emergencies. You
will find yourself
I'd like to add that we use the Aperio system with Ventana's antibody and
it works well. It took a lot of work tweaking of the algorithm to get the
software to score accurately using the 4B5 clone but that was the stain our
pathologists were already used to.
Mark
On Tue, Apr 23, 2013 at 1:50
11, 2013 at 6:26 PM, Cristi Rigazio cls71...@gmail.com wrote:
We have a formaldehyde test kit. It's a dip stick type test.
Sent from my iPhone
On Apr 11, 2013, at 5:31 PM, Mark Tarango marktara...@gmail.com wrote:
Can I ask how you test before dumping?
Thanks
Mark
On Apr 11, 2013 6:21
We use a heat block during digestion. There is less chance of
contamination with a heat block than with a water bath.
... and no we don't use antigen retrieval solution for this! We use a
Qiagen kit too.
Mark
On Wed, Apr 3, 2013 at 9:11 AM, Helen Fedor hfe...@jhmi.edu wrote:
We have been
Hi Matthew,
We use them and they work fine. They are much cheaper too. We've had them
for about 3 years. I initially had some concerns about the DAB being
absorbed and staining the labels on some cases. Our safety person told me
that it's not a safety concern and it's safe to touch the label
The package insert for PathVysion (HER2 FISH) says to bake overnight at 56
degrees C. We never do this and our signals are clear, bright, and
punctate. Many of the slides are just air-dried. Some are baked for about
20 minutes and I don't notice any difference between them. I wonder if I
would
Hi Jim,
We run the control slide that Ventana sends (although not the best control)
but we add a section from our tissue control block before staining. The
tissue control block contains a 0+, weakly staining 1+, and a 3+. We use
the ventana/tissue control slide as our batch control. This slide
I second that!
On Tue, Feb 12, 2013 at 11:12 AM, Robert Schoonhoven
robert_schoonho...@yahoo.com wrote:
All you need to do is have them drain the chamber and place the cassettes
on a nonabsorbent surface and allow them to cool to room temp. On Monday
morning have the techs put the into the
Center
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [mailto:
histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Wednesday, February 06, 2013 2:07 PM
To: Jesus Ellin
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] High
Hi Cheryl,
We use the Aperio system for HER2 scoring. Our lab manager put a cytotech
in charge of validating the digital reading and her next project is ER and
PR. For HER2 IHC, the software is initially set for Dako's Hercept test.
We don't use Dako, we use Ventana staining platform and the
of tumor.
Mark
On Wed, Feb 6, 2013 at 7:03 AM, Bob Richmond rsrichm...@gmail.com wrote:
Mark Tarango notes:
Many pathologists, if they have any doubt about the score will just say
that it is 2+ so that its gets HER2 by FISH which is considered the best
method for determining HER2 status.
On one
If you are just staining the slides and not reading them, then you are NOT
performing high complexity testing. The person who reads the slide is
doing the high complexity part.
Mark
On Wed, Feb 6, 2013 at 11:54 AM, Sara Baldwin/mhhcc.org
sbald...@mhhcc.orgwrote:
Hi histonetters
Is ventana
Just to clarify, this is not my interpretation. This is what CAP will tell
you when you give them a call.
Mark
On Wed, Feb 6, 2013 at 1:07 PM, Jesus Ellin jel...@yumaregional.org wrote:
I would say this is high complexoty testing and the tech performing this
has to have knowledge of the
Am I the only one who thinks its funny Joe the Toe did an unsubscribe
e-mail and Cheryl Kerry copied an entire digest to reply. Oh histonet pet
peeves... haha
On Mon, Feb 4, 2013 at 1:05 PM, Cheryl tkngfl...@yahoo.com wrote:
Oh no, Joe! Say it ain't so!! Please tell us this is just to take
protocol (specially if it is based on DAKO'sprotocol)
should remain as is.
The diagnosis differences should not determine a change.
René J.
*From:* Mark Tarango marktara...@gmail.com
*To:* Wilson A wilson6...@yahoo.com
*Cc:* histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
If anyone has any documentation that says the staining of IHC slides is NOT
high complexity it would help a histonetter out there. I got an e-mail
from someone who is HT(ASCP)QIHC but does not have an AA degree. Their lab
director is threatening their job saying they aren't qualified to do IHC
The staining portion is not high complexity. The reading of the slide is.
On Tue, Jan 22, 2013 at 10:04 AM, Courtney Pierce
courtney.pie...@quintiles.com wrote:
Are IHC high complexity test.
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating the
It can't be used to just pull blocks. The slides have to be reviewed and
the best block chosen by a pathologist. If there is only one block then
the pathologist needs to look at the slides and determine if there is
enough tissue for molecular testing. It's a professional charge.
Use it on
Hi Vicki,
I don't have a protocol but it should be very similar to the HER2 dual ISH.
I'm assuming that you'll be using a probe targeted to chromosome 12 too
and not just doing the MDM2 (but maybe I'm wrong). I would start there.
I'm working on validating MDM2 FISH and it's nearly identical to
Hi Cynthia,
We do PCR for KRAS and BRAF. You cant do these by FISH anyway, the
mutations are too small.
We do FISH for ALK and HER2. For ALK, there is no antibody that can
detect all the positive cases. ALK FISH is the only method that can detect
ALK break aparts regardless of the fusion
Francie,
Conjugated antibodes CAN be detected by secondary antibodies. So the
conjugation doesn't really hide the primary antibody to the secondary.
I've done it and seen it with biotin and FITC labeled antibodies.
Something to consider..
Mark
On Wed, Nov 7, 2012 at 11:43 AM, Frances Elizabeth
Have you tried using more sections during extraction? Can you extract into
a smaller volume?
On Friday, November 2, 2012, Vanessa Orsini wrote:
Hello,
I need to extract RNA for a RT-PCR after Laser Micro
Dissection on xGal stained slides.
I tried using sections from unfixed frozen
Hi Jill,
Have you tried using cases that you've already sent out to other labs and
they've reported as ALK-positive? That is how we validated this assay in
my lab. Most cases aren't very positive (you only have to find 15 cells
out of a hundred that you count to call the case positive if a
...so even if I increase the number
of slides I'll never have a lot of material...
Inviato da iPhone
Il giorno 2 nov. 2012, alle ore 16:44, Mark Tarango
marktara...@gmail.com javascript:_e({}, 'cvml',
'marktara...@gmail.com'); ha scritto:
Have you tried using more sections during extraction
Hi Norm,
It sounds like he had something to contribute. I don't think It shouldn't
matter where he works. Someone else asked the question.
Mark
On Mon, Oct 29, 2012 at 10:00 AM, Norm Burnham norm.burn...@propath.comwrote:
I didn't know that the Histonet site is being used as a medium for
Hi Carol,
Does this person put them in a freezer for 20 mins or so after DAPI
staining? Someone once told me this helps with smearing. I'm not really
sure if its true, but couldn't hurt to try it.
Mark
On Thursday, August 30, 2012, Barone, Carol wrote:
Someone sent me a question regarding
Hi Debra,
Formaldehyde was listed as known to be a human carcinogen in the 12th
Report on Carcinogens (2011) put out by the Department of Health and Human
Services. Here is a link
http://ntp.niehs.nih.gov/ntp/roc/twelfth/profiles/Formaldehyde.pdf
This is what is probably behind any recent
Hi Donna,
We use Dako #M3635 at 1:100. Mantle cell lymphoma is our control.
Mark
On Tue, Jul 24, 2012 at 8:16 AM, Suresch, Donna L.
donna_sure...@merck.comwrote:
Hello Histonetters,
Has anyone done IHC using Cyclin D1 antibody? What vendor was used? What
control tissue was used?
Thank
Hi Cindy,
The one from Dako works well at a 1:200 dilution on the Ventana platforms
(catalog #M7001). We use HIGH GRADE ovarian serous carcinoma as a positive
control. You should get intense nuclear staining throughout the tumor with
this control.
Mark
On Thu, Jul 19, 2012 at 12:25 PM, Cindy
Hi Willem,
H. pylori needs the low pH of the stomach to survive and grow. It won't
naturally be in lung tissue. When looking for HP, it's found on the mucosa
and gastric pits of the stomach. Since you're looking for it in a specific
place, I think putting it in lung is a bad idea.
Your best
I would think that if you're billing the client and not the insurance that
you could charge per block for the technical. After all you're just
providing the stain to them. In my opinion, the client should eat this
cost. I would let the client know that you'd be billing this way before
staining
I'm trying to think this through. Like Will said, if it can be shown that
you can charge immunos per block... I thought we could only charge IHC per
specimen these days. Wouldn't each stage be a different specimen? I would
think billing per block of the same stage would be over charging. Why
Carol,
Its nice to hear this isn't a regular thing. In reading your original
question, it sounded like you were excited to be charging five times for
those immunos and you were ready to argue for it. Apparently that was not
the case, you wanted more information and thoughts on the subject.
I
FISH should be scored at high power under oil immersion (60-100x
objective). If they are scoring at 40x, it could be a problem.
good luck!
Mark Tarango
On Tuesday, June 5, 2012, Eric Hoy wrote:
We do a LOT of fluorescent microscopy in our immunology lab, so I have a
bit
of experience
I think normal breast staining of myoepithial cells is the best control for
this antibody. We use BioGenex's antibody (product # MU333-UC) at 1:200
on the Benchmark XT (CC1 mild, 8 mins primary antibody and ultraview DAB
detection).
Mark
On Fri, Mar 16, 2012 at 7:34 AM, Rene J Buesa
Is the humitity causing any problems or is the number just not what you're
used to seeing when you look at the readout?
On Tue, Aug 30, 2011 at 10:08 AM, Jill Cox jco...@yahoo.com wrote:
Hi Histonetters!
I am having humidity issues in a new lab in Long Beach Ca. It's 68%
humidity inside lab.
How are you doing your ISH? A commercial manual kit, home-made kit,
instrument?
On Fri, Aug 5, 2011 at 8:31 AM, Johnson, Nacaela
nacaela.john...@usoncology.com wrote:
Would anyone be willing to share their protocols for the Kappa and
Lambda ISH? I cannot get rid of the eosinophilic
We're sticking to our IVD CD117/c-kit rabbit poly from Dako.
Mark
On Fri, Jul 29, 2011 at 10:42 AM, Vickroy, Jim vickroy@mhsil.comwrote:
We are finding that the FDA approved protocol from Ventana does not stain
some definite gi stromal tumors that stain quite well with another vendor's
We get ours from Becton Dickinson, product #349205.
Mark
On Thu, Jul 28, 2011 at 9:23 AM, Cynthia Pyse cp...@x-celllab.com wrote:
Hello Histonetters
What company is everyone buying their Cam 5.2 antibody from? Thanks for the
information.
Cindy Pyse, CLT, HT (ASCP)
Laboratory/Histology
I should have mentioned...we use it at 1:20 dilution.
Mark
On Thu, Jul 28, 2011 at 9:27 AM, Mark Tarango marktara...@gmail.com wrote:
We get ours from Becton Dickinson, product #349205.
Mark
On Thu, Jul 28, 2011 at 9:23 AM, Cynthia Pyse cp...@x-celllab.comwrote:
Hello Histonetters
Hi Linda,
I had simliar trouble before I bought and added the extra HybReady to the
protocol (there is one that comes with the detection kit, but you need two
for good staining). It will clean up your stain and A LOT and remove those
blue splotches that show up when you don't use it.
Mark
On
Could anyone suggest suituble antibodies for the following markers in dog
and cat tissue:
Vimentin, Pankeratin, CD3 and CD20, CD18, MelanA, Factor VIII
Also could use a suggestion for C-kit in Dog tissue only.
thanks
Mark
___
Histonet mailing list
That's funny that you got cited for that. I was surprised to learn what our
safety officer setup at my lab for paraffin disposal. A commercial company
takes our paraffin and makes a product with it that is mixed with cedar
sawdust and paraffin wax for starting fires in the BBQ, fireplace, or
We do put controls on each slide in a case. Sometimes it's just one slide
that failed in a run. A batch control wouldn't tell you which slide failed
if there are no internal controls in the patient tissue. I personally
wouldn't feel comfortable doing IHC with batch controls.
Mark
On Wed, Jul
Hi Toni,
We put the control on the same slide and after it's stained and coverslipped
we draw a line to seperate the control and patient tissue with each side of
the line having either a C or P written on it.
Mark
On Tue, Jul 19, 2011 at 12:27 PM, Rathborne, Toni
Hi Anita,
We ran 40 cases and sent the same blocks to Phenopath for them to
run for comparision. There is a requistion for doing an ER/PR validation on
their website.
Mark
On Tue, Jul 19, 2011 at 1:43 PM, anita dudley azdud...@hotmail.com wrote:
I know this has been talked about before here
My lab uses the SPT24 clone for TTF-1. Let me know if you'd like any
protocol info.
Mark
On Thu, Jul 14, 2011 at 11:47 AM, Inman, Anna anna.in...@stmarygj.orgwrote:
Is anyone using the SPT24 clone for TTF-1 using the Ultraview platform
on the Benchmark XT?
Our pathologists have read this
We run the H. pylori IHC stain on all stomach specimens to improve turn
around time; however, when an inflammatory background is not appreciated by
the pathologist the charge is removed before sign-out and we eat the cost of
producing the slide.
Mark
On Wed, Jul 13, 2011 at 11:37 AM, Richard
The only problem here is that the Cell Marque antibody is currently on
backorder until at least the end of August from what I've heard.
Mark
On Thu, Jul 7, 2011 at 12:36 PM, Angela Bitting akbitt...@geisinger.eduwrote:
CC1 standard, 32 min incubation with heat disabled. I use Cellmarques
protocol. Then again, even deparaffinzing the slide using xylene
is off label, since it was approved using Hemo-D substitute. I imagine it
would be harder to go off label with Ventana's software. I guess you've
got to weigh the pros and cons as with everything.
Mark Tarango
On Sun, Jul 3, 2011 at 2
Hi Marcia,
Our slides for the Her2 CAP survey stained just fine. We're using a Ventana
XT to stain for Her2 by IHC.
Mark
On Wed, May 4, 2011 at 9:54 AM, Marcia Funk fu...@mercyhealth.com wrote:
CAP validation Her/2 slides not staining as clear as before. Is anyone
else having any issues ?
Hi Dana,
Invitrogen sells a rabbit polyclonal anti-pax-2 antibody that works well on
FFPE tissues (Cat # 180483). We use a multi-tissue control block for a
control. It has normal kidney to show the endothelial cells around
glomeruli and renal tubules staining, clear cell renal cell Ca to show
If anyone has the catalog numbers for the probes, that would help. I
couldn't get this info from Ventana when I asked. They said we have to ask
another lab, even for catalog numbers!
Mark
On Tue, Apr 19, 2011 at 7:59 AM, Sheila Fonner sfon...@labpath.com wrote:
SAME HERE!!! I would love
Well I wouldn't try and use a Ph.D. in religous studies to qualify for high
complexity testing...
On Wed, Mar 16, 2011 at 4:36 PM, Mark Turner mtur...@csilaboratories.comwrote:
Regarding CAP checklist, question ANP.23041. The operation of the imaging
system is performed by high-complexity
Hi Jim,
If it's a new clone then a new valiation should be performed. I know of
many instances where one clone stains something that another doesn't.
If this is regarding Ventana not selling certains clones, those clones are
still available from Cell Marque directly.
Mark
On Mon, Mar 14, 2011
We do use the Ventana antibody for gastric cases. There is some difference
in reading the slide for the pathologist. I'll send you an article on it in
a seperate e-mail (so I can attach it).
Mark
On Fri, Mar 11, 2011 at 8:54 AM, Coppin, Margaret copp...@aruplab.comwrote:
Hello,
I am
So cruel!
On Fri, Feb 25, 2011 at 8:13 AM, sgoe...@mirnarx.com wrote:
It's sunshine and 75 here in Texas...nanny nanny...I do love my state's
weather!! We had shorts on yesterday =)
Sarah Goebel, BA, HT(ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin,
Hi Milton,
We use Biocare's Universal Negative Control Serum in place of the antibodies
in the negative control staining protocol. We use normal prostate tissue to
show lack of p504s staining (to make sure it isn't overstaining normal
glands). The prostate cancer in the tissue control shows
Its true, that test is too easy if you ask me. Dako handbook is all you'll
need.
Mark
On Thu, Feb 10, 2011 at 5:46 AM, Houston, Ronald
ronald.hous...@nationwidechildrens.org wrote:
Read and digest the Dako book and you will sail through the exam. For a
specialist type qualification it
Hi Mike,
Did you wash the bottles and rinse them and then pump the water through the
instrument before adding reagents and purging all again? We had a problem
like this about 2 weeks ago. I think someone loaded the wrong bulk reagent
onto the instrument. Cleaning it, purging with water and
Hi Paula,
You mentioned the staining in a liver specimen using the cell marque
antibody against TTF-1. The clone named 8G7G3/1 that cell marque sells is
known to stain liver cells and liver cancer. Your pathologist might be
interested in knowing that. Here is a reference you can give him:
...@wvhcs.orgwrote:
Pax-2 (rabbit poly) is available from Cell Marque but in our experience it
also has some cytoplasmic background staining.
Mike Dessoye
---
Message: 21
Date: Tue, 4 Jan 2011 16:06:56 -0800
From: Mark Tarango marktara...@gmail.com
Subject: [Histonet] Pax-2
To: histonet
background
staining that I've seen?
I'd appreciate any info at all.
Thanks
Mark Tarango
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Hi Gloria,
Are you still looking for some good HP controls? I could send you a block
of one of our controls if you're intersted. I have blocks from
several different specimens. Just let me know your address and fedex
number.
Thanks,
Mark
On Sun, Nov 21, 2010 at 12:30 PM, Gloria Cole
Hi Justin,
Do you run the p53 on a cell block or a cytospin or something different?
Mark
On Mon, Nov 8, 2010 at 12:36 PM, Justin Peters
jpet...@bostwicklaboratories.com wrote:
We receive requests from our pathologists for p53 IHC on urine but I am
unsure as to what controls to use. We
Hi Melissa,
We use it on bone marrow biopsies all the time on the Ventana XT. We made
sure to include BMBXs in the validation. We do get strange staining from
time to time, so it's particularily important to look at the negative
control for these.
Mark Tarango
On Tue, Oct 19, 2010 at 7:49 AM
Hi Vikki,
It's best to run it all together and run a negative control for each
detection kit.
Mark
On Fri, Oct 15, 2010 at 7:25 AM, Victoria Baker bakevicto...@gmail.comwrote:
Hi
I have a hypothetical question to those who run IHC on Ventana instruments.
Are you running your negatives with
Hi Sarah,
It's better to have the control on the same slide. There are slides that
work and slides that don't. You know which ones are good and which ones are
bad because you have that control on each slide. It's not always a complete
run that fails. Yes, you CAN have a single batch control
* *I think the chatter just died down since many of us are at the NSH
symposium.
Mark
On Wed, Sep 29, 2010 at 8:49 AM, Feher, Stephen sfe...@cmc-nh.org wrote:
I am having some issues with receiving email from the listserv. I was
successfully receiving email and it suddenly stopped. If I
Hi Jessica,
Our control for this antibody is normal lung, adenocarcinoma of the lung,
and thyroid. We use this same block for other stains too (TTF-1,
sufactant-A, EMA, etc.) but if I were going to make a control block specific
for this antibody, I would want to include a squamous cell carcinoma
Hi Nita,
I agree that buffer should be used as the negative control reagent. If you
have a seperate slide that is cut and put into buffer, just coverslip it
with the same mounting media and you have a negative control. All the
instrument is doing is putting on the antibody and then rinsing it
Here I am answering my own question. I just remembered where I used to get
this antibody. It's from Millipore/Chemicon.
Mark
On Mon, Aug 9, 2010 at 7:53 AM, Mark Tarango marktara...@gmail.com wrote:
Hi Histonet,
Does anyone use an Anti-IDO on FFPE tissues? Would you please let me know
I've been told by a Biocare Salesperson that the BC4A4 clone is the same
exact clone as 4A4. The BC in front of 4A4 just means Biocare. I don't
think that Ventana sells a concentrate of this antibody. You'd want a
concentrate for your PIN4 so you're probably better off sticking with your
Ethanol/alcohol is what will process the specimen. If the tissue is fixed
would it really matter that the tissue came into contact with ethanol?
Mark
On Tue, Jul 27, 2010 at 11:03 AM, Hayes, Randi (HorizonNB)
randi.ha...@horizonnb.ca wrote:
At a recent conference, our PA learned of using GEWF
Hi Cathy,
I'm using their p63, but on the Ventana XT with ultraview DAB detection. We
use it at 1:200 and get a strong signal in both breast and prostate
tissues.
If you haven't tried staining various cases of prostate, I'd suggest it.
Sometimes I'll get a prostate that stains the basal cells
Biocare has a rabbit polyclonal anti-HP ab and a mouse monoclonal. Which
one do you use?
Mark
On Wed, Jun 30, 2010 at 8:05 AM, McMahon, Loralee A
loralee_mcma...@urmc.rochester.edu wrote:
Use the RTU from Biocare. It is clean and easy. You can use it with the
Dako Flex Kit.
Loralee
You'll have to use prep kit stickers and duplicate protocols to do it, but
it is possible. You just need to copy the protocol and save it as another
number, then change the primary antibody to a prep kit sticker save again
and then put that sticker on the dispenser. Then you need to print
Hi Phebe,
I can't be sure about this since you didn't post your protocol, but
alpha-SMA is an antibody that does not require antigen retrieval. If you're
doing some kind of retrieval, I'd suggest trying it without.
Thanks
Mark
On Wed, May 26, 2010 at 6:56 PM, Phebe Verbrugghe
I just realized the question came from Belgium. I have no idea how they do
things there.
Mark
On Thu, May 20, 2010 at 2:14 PM, Mark Tarango marktara...@gmail.com wrote:
Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if
you do HER2 the maximum is still 48 hours. I'm
Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if
you do HER2 the maximum is still 48 hours. I'm assuming you want HER2 as
well, so your best option would probably be to hold the tissues in 70%
alcochol on the processer until Sunday night.
Mark Tarango
On Thu, May 20
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