Dear Charles,
Can you provide more details about your needs? We might have gentler
decalcifying solutions than the usual ones. Do you need histology services for
your mouse skulls? We can handle a wide range of routine and special
histological needs. Could you tell us where on the skull you
Hello all IPS/IPS users,
we used both printer, IPS and IPC just with the original printer driver and a
database-application which offers us much more options than the application
that comes with the printers (NiceLabel - as far as I know). So I think it
should be possible to reinstall the
Hello Merissa,
You can use a diamond band saw, as they are produced by EXAKT. This special
technique will cut soft tissue quite smooth without destroying the tissues. So
you can cut slices with a few centimeter thickness which can be fixed in
formaline after sectioning.
What kind of
Dear Kate,
very interesting question.
The both substances you mention are a bit difficult in this respect. In a
general crystal structure phosphortungstic acid has 44 water molecules and
phosphormolybdic acid 28 molecules of crystal water. This would mean, that for
a an exact 5% solution you
Dear Kimberly,
we have two systems here in our lab, both with microscopes from ZEISS. The
first one is a TissueFaxs-System which is for scanning of maximum 8 Slides and
special optical requirements (polarization, phase contrast, etc.). This system
is quite good and flexible and you can use
The sirus-red stain is a stain to differentiate collagene I against collagene
III in polarized llight, so in bright field all fibres will be red, just if you
change to polarised light, you can differentiate both kinds of fibres by their
birefrigence which is orange to yellow for collagene 1 and
There is also a Kit and the ready to use solution available from MORPHISTO
Germany.
Just contact the sales office: i...@morphisto.de
Cheers
Michael
> Am 27.07.2019 um 23:53 schrieb Tony Henwood (SCHN) via Histonet
> :
>
> Methods found here:
>
>
Hello Jennifer,
I do not have experiences with elephant tissues, but with other tissues that
have thick connective fibres. Sodium hydroxide may help, however, I would
recommend resin embedding, too. For this purpose Technovit 7100 would be a good
and easy to use resin. Its easy to infiltrate
Hello Histonetters,
since the summer in Germany is recently quite warm I want to ask about your
experiences with specimen cooling during and before sectioning via microtome?
Does anyone use Leica CoolClamp or similar cooling units? What do you think
about this? Our experience is, that cooling
Hello Jessica,
Hello Natalia,
we have discussed the question of bone staining methods in our lab and we would
recommend the following stainings, which will work on resin embedded material:
(1) For an overview and differentiation of various types of bone the
traditional MASSON-GOLDNER or AZAN
Hello Jessica,
we have a special „bone stain“ staining solution in our portfolio which is
exactly for the purpose you described. Osteoid will be stained green or red to
dark red, incomplete mineralized bone bone light red or orange yellow and the
demarcation zone light green. Its also possible
Dear Jessica,
the best way to embed screws or any other implant material or bones or teeth or
any compounds is to Technovit 7200. It a single component Acrylate that hardens
in blue and white light within a few hours.
We are the exclusive distributor for all Histology Technovits from Kulzer,
Dear Jessica,
what kind of MMA have you used? If it is Technovit 9100 we have an established
procedure here in our lab. How many slides do you have? We can make you an
offer to stain them here in our lab for you. There are several trichrome or
polychrome stains possible.
Best regards
Michael
Dear Tasha,
well, I think you should modify your protocol:
(1) don’t use microwave
(2) wash in ethanol after Bouin and not in water (70 - 80 %)
(3) tap water as long as Weigert working solution
(4) Bieberich Scarlet is not the correct stain for Masson, you should use Acid
fuchsine + Ponceau 2R
Dear Jessica,
fixation should be at least 48 hours, however it is not problematic if the
tissues stay longer in these solutions. Important point is, to remove the
picric acid, which is a component of the Bouin solution, with several steps of
70 - 80 % of ethanol, so after fixation go directly
Dear Jessica,
we have done embedding in paraffine and the results are quite good. The
critical point is fixation. Due to the high proportion of water in the tissue,
the best fixation is possible with Bouin-solution or - if you want to do IHC
stainings, Zamboni-fixation (which is a variant of
Well, in Germany, and as far as I know in Europe, most labs use ethanol at 96
and 99 percent as the last two steps in tissue processing. These ethanols are
are denatured with MEK (= Butanon, Methylethylketon). Methanol or isopropyl is
not allowed as denaturation agent in Europe, otherwise you
Hello Nancy,
we have done some histology on sponges and tried paraffin and resin embedding.
Due to the spicules and also biofilms and sand etc. the best results could be
reached with resin embedding in technovit 7100 or better 9100 or 7200 with
cutting grinding techniques. Sectioning of 9100
Hello Sandra,
if you need very high quality pathology saws we recommend the machines from
EXAKT. We have three of them in our lab and use them for pathology sectioning
as well as for cutting grinding techniques. They work with a diamond band and
there are several kinds of diamond bands
Hi Dorothy,
Hi Reuel,
MMA embedding and deplastination is of course a very difficult process and many
protocols are discussed, but only a few work well. So in general: real
deplastination cannot be performed by xylene or acetone alone, these solutions
are just a pretreatment. The best way for
Dear Mama,
there are several elastic stains which are as brilliant as Verhoeff.
For a very quick and easy stain you can try:
Van Gieson with Resorcinfuchsin or
Resorcinfuchsin with Thiazin-picric-acid
Also very interesting results can be generated with:
Aldehydfuchsin-Tripple Stain
and
Hello Histonetters,
I am not sure if this question reaches the right persons here, but I try to
place my question here:
We have purchased a complete TissueFaxs-Akquisition and Analysis-System from
TissueGnostics a few years ago for a research project. However, the project is
finished for
Hello Histonetters,
I am not sure if this question reaches the right persons here, but I try to
place my question here:
We have purchased a complete TissueFaxs-Akquisition and Analysis-System from
TissueGnostics a few years ago for a research project. However, the project is
finished for
Hello,
we process heart and other biopsies of muscles in Bouin oder Bouin-Hollande
fixative which both also work fine with IHC and give very brilliant results
with any kind of trichrome stainings.
With best regards
Michael
> Am 06.09.2017 um 18:22 schrieb Blanca Lopez via Histonet
>
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