Re: [Histonet] Tissue falling off slides

Hi Annette! Do you put anything else in your water bath besides deionized water? Richard CartunMorphologic Proteomics, LLC(860) 490-7633 On Tuesday, January 30, 2024 at 03:14:50 PM EST, Foshey, Annette via Histonet wrote: I work with whole mount prostate blocks that I cut and stain

Re: [Histonet] Red chroamgen/LeicaBond andBondMax

Yes; we run IHC tests everyday on our Bonds with Leica's "Red" detection kit. You do need to be careful when dehydrating the slides prior to coverslipping since the chromogenic product is not as stable as DAB. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin

Re: [Histonet] Histonet Digest, Vol 220, Issue 8

ts, especially those that are not being produced any longer. Also, with the current staffing crisis in Histology/IHC laboratories and supply-chain issues we need to simplify our work environment. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morpholog

[Histonet] Pneumocystis Control Tissue

I apologize someone contacted me several weeks ago about the availability of Pneumocystis control tissue, and I misplaced his name and contact information. Please contact me again if you are still in need of control tissue. Richard Richard W. Cartun, MS, PhD Director, Histology

Re: [Histonet] Dr Freida Carson

I'm very sad to learn of her passing. She was a remarkable person. I didn't know her well, but I always enjoyed speaking with her at meetings and learning from her. Many years ago I bought her entire collection of bound "Histotechnology" journals. Richard Richard W. Cartu

Re: [Histonet] Microwave slide drying

fference. Also, how would you monitor the temperature of the slide? However, my philosophy has always been if it makes the process more efficient and/or less costly without changing the outcome, "do it". Happy Thanksgiving to all who celebrate it. Richard Richard W. Cartu

Re: [Histonet] release of body parts

(funeral home/mortuary) must sign the release form. Please keep in mind that each state may have statues on the release of human tissue to patients or their families. I also know that some towns here in CT have ordinances preventing residents from burying human tissue on their property. Rich

Re: [Histonet] HSV1 and HSV2

Hi Martha: We are using type-specific monoclonals from BioSB (Santa Barbara, CA). They also sell a cocktail of the two antibodies which is what we use for screening. Rarely, do we get requests for type-specific identification. Richard Richard W. Cartun, MS, PhD Director, Histology

[Histonet] Question - Bone marrow core biopy specimens

are implementing a protocol today where we will cut "15" slides upfront; 3 H at different levels and 12 unstains for potential histochemical stains and/or IHC tests. Are we crazy? Thank you, and I hope everyone is doing well. Richard Richard W. Cartun, MS, PhD Director, Histology &

Re: [Histonet] Antibody diluent

Interesting . I will definitely check this out. I know I'm "Old School", but I have never "personalized" the diluent for preparing antibody dilutions. We have always used just one diluent for all the antibodies that we have run over my 43-year career. Richard Richar

Re: [Histonet] Controls

you cut your control sections unless the case has multiple blocks showing the same pathology. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anat

[Histonet] Formalin fixation for COVID-19 positive tissues .....

can share on this subject would be appreciated. Thank you and stay safe. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospi

Re: [Histonet] BRAF testing

). I would be interested in knowing what others' experiences are. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hosp

Re: [Histonet] IHC troubleshooting

Are you using some form of antigen retrieval? In addition to what has been discussed previously, antigen retrieval using heat (HIER) can cause tissue loss especially collagen. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphol

[Histonet] Cellient for cell block preparation

! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office)

Re: [Histonet] PLAG1

Looking at an article in Histopathology (January 2018) on PLAG1 IHC testing, the authors used clone "3B7" (Novus Biologicals in Littleton, CO) on the Bond Max IHC platform form. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopatholo

Re: [Histonet] Cell block processing

or RPMI in formalin. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102

Re: [Histonet] Chromogenic ACD RNA ISH probes

We are currently running RNAScope for HPV and Albumin on our Leica Bond IIIs. It's a wonderful technology! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assis

[Histonet] Tissue Contamination

What are people doing to ensure that there is no tissue carry-over on instruments between cases when grossing? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collec

[Histonet] Major skin resections - CPT coding

was coding these as "88305" and I don't think that is appropriate. Thank you in advance for your comments. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Prog

Re: [Histonet] [External] Re: SDHB IVD antibody

We use the antibody at a dilution of 1:50 following low-pH HIER (20 minutes) on the Leica Bond Max IHC platform. Our primary antibody incubation is 15 minutes. Detection is Bond Refine HRP with DAB. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman

Re: [Histonet] SDHB IVD antibody

Cell Marque offers an excellent SDHB rabbit monoclonal (clone EP288) that we use in our laboratory. However, it is not an "IVD". Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospeci

[Histonet] H Artifact

We had several cases last Friday that showed "optically clear nuclei" (primarily lymphocytes) on H Have you seen this artifact and, if so, what is it due do? I will be happy to e-mail a photo to anyone interested. Thank you as always for your help. Richard Richard W. Cartu

Re: [Histonet] ER/PR controls

itive control! When I look at my positive controls on a daily basis, I am looking for reproducibility in the immunoreactivity in the tumor and the benign epithelium. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics La

[Histonet] Ventana p16 antibody

We use the Roche/Ventana p16 antibody (clone E6H4(tm)) on the Leica Bond Max. We are having a difficult time trying to order the antibody. Can someone verify the catalog number (705-4713) for me? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman

[Histonet] Question for Leica Bond IHC Users

controller; however, we have been told that we cannot connect our 6th instrument unless we get a second controller (and that's not going to happen). Has anyone else been in a similar predicament? And, if so, how have you resolved it? We need to use all "6" instruments. Thank you.

Re: [Histonet] Derm IHC question

Doesn't sound like a fixation issue to me. Could the tissue be drying out before it's placed in formalin? Also, are these specimens inked for assessment of margins? I've seen ink interfere with immunoreactivity. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Be

Re: [Histonet] ER/PR question

controls, and the HER2 is negative, I recommend that the testing be repeated on excisional tumor for confirmation. This is why it's important to submit adjacent benign breast tissue along with the tumor tissue when grossing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Mart

[Histonet] IHC Billing - 88342 vs. 88341

elect the appropriate one for billing. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT

[Histonet] GD2

Hello everyone. Is anyone doing IHC testing for "GD2" on FFPE tissue? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Dire

[Histonet] IDH1 Antibody

If you are doing IHC testing for IDH1 here in the United States, do you get the antibody (clone HO9) directly from Dianova in Germany or is there a US distributor? I did try HistoBiotech, but they no longer carry Dianova antibodies. Thank you. Richard Richard W. Cartun, MS, PhD Director

[Histonet] Lyme Disease

Is anyone doing PCR testing for Borrelia burgdorferi (Lyme Disease) from formalin-fixed, paraffin-embedded tissue? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Laboratory Director, Biospecimen Collec

[Histonet] IDH1

For those labs that are performing IDH1 IHC testing on FFPE tissue, which clone are you using and where do you get it? Thank you. Happy Holidays to everyone. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology/Morphologic Proteomics Labora

[Histonet] Question - EM

How long can tissue remain in glutaraldehyde before EM testing is performed? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director,

[Histonet] Alizarin Red Stain

Does anyone do the Alizarin Red histochemical stain for calcium in clinical samples? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant

Re: [Histonet] Unstained slides

processing. Xie R, Chung J-Y, Ylaya K, et al.: Factors influencing the degradation of archival formalin-fixed, paraffin-embedded tissue sections. J of Histochem Cytochem 2011; 59:356-365. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopatho

[Histonet] Decalcification for Bone Marrow Biopsies

. Someone told me this morning that the company producing this product was sold and the formulation may have changed. Is that true? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Bio

Re: [Histonet] LEF1 - antibody

We use a RTU (clone EP310) from Cell Marque on the Leica Bond. Antigen retrieval is "high pH" HIER for 20 minutes. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen C

[Histonet] COnsult Reporting

I know I asked this question before, but I can't find my notes. If you provide a diagnostic consultation on pathology slides, do you need a separate report with a new "Date-of-Service" (the date that the consult is requested) for reporting and billing purposes? Thank you. Richard

Re: [Histonet] Cold Ischemic interval

We are using the "ASCO/CAP" guidelines for all cancer resection specimens. Richard Richard W. Cartun, MS, PhD Director, Histology and the Martin M. Berman, MD Immunopathology and Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic

Re: [Histonet] Extra unstained paraffin embedded tissue slides

We file our unstains separately and then discard after one year. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology

Re: [Histonet] Beta-Amyloid antibody for IHC

No, I would like to use “6E10” if it’s still available. Thank you! Richard From: Colleen Forster [mailto:cfors...@umn.edu] Sent: Wednesday, December 27, 2017 4:23 PM To: Cartun, Richard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Beta-Amyloid antibody for IHC CAUTION

[Histonet] Beta-Amyloid antibody for IHC

We are looking for a good antibody to detect Beta-Amyloid in formalin-fixed, paraffin-embedded human brain tissue. Any recommendations? We have been using the clone "6E10" which we purchased from Signet Laboratories back in 2001. Thank you! Richard Richard W. Cartun, MS, Ph

Re: [Histonet] ROS1

Yes, we've had excellent results using their clone "D4D6". We use it at 1:50 dilution on the Leica Bond Max following high-pH retrieval. Please contact me directly if you require additional information. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin

[Histonet] I am trying to contact ....

Diane Cadorette-Hall. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, C

Re: [Histonet] EDTA decalcification tissue issues

loss. We are only using EDTA for small biopsies. Please not that these specimens must be fixed adequately before the decal process is started. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory

[Histonet] Announcing the 12th annual retreat of applied immunohistochemistry and molecular pathology at the beautiful Marriott, Key Largo, FL

I am forwarding this exciting meeting announcement to the Histonet Community. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic P

[Histonet] Mass Spectrometry

Anyone working in Anatomic Pathology and using mass spectrometry for protein analysis on cancer cells? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection

[Histonet] autostainer slide stainer IHC repair manual

Hi dear Histoneters, I am looking for a copy of autostainer slide stainer IHC repair manual. (my request is about where could be a sensor in the arm of the Y position (back to front) ?) Thanks R W ___ Histonet mailing list

Re: [Histonet] CD35

We are now using Epitomics' clone EPR6602 with great results. I don't know if it's offered as a "RTU", but I would check with Cell Marque or abcam; they carry Epitomics' clones Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology

[Histonet] ClearRite

Anyone using ClearRite (xylene replacement) for tissue processing? If so, what's been your experience? Any effect on IHC or molecular testing? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics La

[Histonet] Question

. We would not have a consult accession number (or report) here at our hospital for this specimen. Does this make sense? Is anyone else in a multiple hospital system already doing this? I welcome all comments. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology &

[Histonet] IHC testing for Parvovirus (Human)

If you do this test, where do you get your antibody? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology

Re: [Histonet] Send-Outs

Do you have a Pathology Residency Program? If so, good luck with a policy that states that requests will be sent out in 48 hours. Our policy is 3-5 business days once we receive the request. Having said that, most requests are processed in 2-3 days. Richard Richard W. Cartun, MS, PhD

[Histonet] NGS Testing

I know this question is somewhat out of our domain, but are any of you engaged in "Next Generation Sequencing" (NGS) testing on patient FFPE cancer specimens? If so, what's your experience with insurance pre-certification and reimbursement? Thank you as always. Richard Richard W.

[Histonet] Pathology Slides Send-Outs

I'm wondering if any of you take a patient's credit card to pay for the FedEx shipping of pathology slides when they request that their slides be sent to another hospital or medical institution? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman

[Histonet] ICD-10 Codes

What do you do when you receive a pathology consult and it is missing an ICD-10 code? I am being told that we cannot accession it until we get a code from the submitting doctor's office, and that we cannot add one. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology

[Histonet] IHC for GAB1 and YAP1

Anyone out there doing IHC testing for GAB1 and YAP1 to subtype medulloblastoma? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant

Re: [Histonet] Return of Gross Only Specimens for Patient Use

, and they must sign a release. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, C

Re: [Histonet] Immunoperoxidase Protocol on Cytospin

r cytospin slide. If you don't get any immunoreactivity it could be a "False Negative" due to pre-analytical variables. I think it can be interesting to try experiments like this, but it's not the way to do IHC testing. Richard Richard W. Cartun, MS, PhD Director, Histology &

[Histonet] Serotonin

Can anyone recommend a good anti-Serotonin antibody for diagnostic morphologic proteomics testing (aka, Immunohistochemistry)? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Bio

[Histonet] BRG1 IHC Testing

Is anyone doing clinical IHC testing for BRG1/SMARCA4? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology

[Histonet] IHC for ALDH1

Is anyone doing IHC testing for Aldehyde Dehydrogenase 1 (ALDH1)? Evidently, it is a prognostic maker used for breast CA. Happy Holidays everyone! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory

[Histonet] Aluminum Testing

Thank you and "Happy Holidays" to everyone. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymou

[Histonet] Hard copy pathology reports

e you done to make sure all care-givers get their pathology reports? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic

Re: [Histonet] ER/PR Uneven Staining

Can you provide more details about the uneven immunoreactivity? Are the negative cells located at the periphery of the specimen or are the negative and positive tumor cells mixed together throughout the specimen? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Be

Re: [Histonet] processing cell blocks in formalin

Yes, I will send you the procedure that we use. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80

Re: [Histonet] Billing Questions

I can only speak to IHC billing. My staff (now 2 people) in our IHC Lab does all the technical IHC billing manually. We average about 3,500 billable IHC tests per month now. The volume used to be higher, but our healthcare system sold most of our outreach business. Richard Richard W

[Histonet] Formalin in Physicians' Offices

Do physicians' offices need to have procedures/policies "on-site" regarding formalin if they put patient biopsy specimens into the small formalin containers? What about formalin spill kits? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin

Re: [Histonet] Vacuolated and torn?

Microtomy is so important, but often over-looked. You might try giving the paraffin block(s) to different people and have them all cut H, and then have your pathologist compare them. If everyone's sections are suboptimal, then the problem is with fixation and/or processing. Richard Richard

Re: [Histonet] Kappa and Lambda ISH

Yes, the CAP now has a PT survey for HPV, EBV, and Kappa/Lambda in situ hybridization (ISH). Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant

Re: [Histonet] CAP ANP.22970 Query

patient demographics, and the antibody clones and IHC detection used. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology

[Histonet] Low temperature freezer

Our ancient "SoLow" -80 degree C. freezer died over the weekend. What do you recommend as a replacement? Do you prefer a chest vs. stand-up? We use it primarily for tissue storage. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD I

[Histonet] C4d IHC in myocardial biopsy

Are any of you an expert with C4d IHC in myocardial biopsy tissue? We had a specimen this week that showed a pattern of immunoreactivity that I am not familiar with and I need to find out if this represents a specific disease process. Thank you. Richard Richard W. Cartun, MS, PhD Director

[Histonet] Testicular biopsy for infertility

What are people fixing testicular biopsies in to evaluate infertility? In the past, I believe fixatives such as Zenker's and Bouin's were used for this purpose since they enhance nuclear detail. Obviously, those fixatives can no longer be used. Thank you. Richard Richard W. Cartun, MS, PhD

Re: [Histonet] P16

We purchase the antibody from Ventana and run it on our Bond Max platforms. During re-optimization, we found we could dilute it 1:10 (15 minutes) following "H2" (high pH) retrieval and still get excellent results. Richard Richard W. Cartun, MS, PhD Director, Histology and the Martin

[Histonet] FW: Question - CoPath

Make that "Sunquest" CoPath .. Richard -Original Message- From: Cartun, Richard via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, May 24, 2016 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question - CoPath We will be switching f

[Histonet] Question - CoPath

We will be switching from Cerner CoPath to Sunrise CoPath in the near future (part of our Epic conversion). Is there anyone using Sunrise CoPath that uses a prostate diagram in their report to demonstrate the absence or presence of cancer in the different quadrants? Thank you. Richard

[Histonet] Date-of-Service for Consults

I have always maintained that the "Date-of-Service" for a pathology consult is the date that the pathology consult is initiated or requested. Is that correct? Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Pro

Re: [Histonet] GLOMERULI ADEQUACY

. However, I don't recommend obtaining cores this long. We have not had to repeat a biopsy over the last 25 years . Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection

Re: [Histonet] Validating IHC on decalcified specimens

I have a validation file for ER, PR, and HER2 IHC testing on decalcified bone specimens with metastatic breast cancer. I compared the results with other specimens from the same patient (usually the primary breast cancer) that were not decalcified. Richard Richard W. Cartun, MS, PhD Director

Re: [Histonet] tests on old cases

If the request is received more than "30" days from the original date-of-service, we re-accession the specimen, and bill and report the test result(s) under the new accession number. The requesting MD now becomes the submitting MD. Richard Richard W. Cartun, MS, PhD Director,

[Histonet] Slide scribe

Years ago, I obtained a metal scribe for etching glass slides, but I can't remember which Histology company I got it from. Any suggestions? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics La

[Histonet] IgG4

I am curious to hear which clone people are using to detect IgG4-positive plasma cells in FFPE tissue. Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection

[Histonet] IgG4

Hi everyone. Which antibody (clone) are you using for the immunohistochemical detection of IgG4 in FFPE tissue? I just found out that the clone that we were using is no longer available. Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman

[Histonet] Sunquest CoPath

to me in a conference call earlier this week. As always, thank you for sharing your knowledge and experiences. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs A

[Histonet] Shipping paraffin blocks overseas

It's been a long time since I have sent a paraffin tissue block out of the country. Can someone bring me up-to-date on requirements for shipping paraffin tissue blocks overseas (specifically Germany). I believe FedEx will be used for the shipping. Thank you! Richard Richard W. Cartun, MS

[Histonet] H. pylori control tissue

Nancy Schmitt from Dubuque, IA asked about control tissue for H. pylori. I have tried e-mailing her, but my e-mail was returned as "Undeliverable". Nancy, please contact me. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology

[Histonet] Patient identifiers

Thanks to all who responded to my question on patient identifiers for prostate biopsies. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director,

[Histonet] Patient identifiers

What are people accepting for the "two" identifiers on the small formalin bottles that are used for prostate biopsy specimens? Can you accept the patient's initials in place of the full name? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin

Re: [Histonet] Histology Workload

from a lab assistant that could help with accessioning, billing, and send-outs. Richard Richard W. Cartun, MS, PhD Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic Proteomics Laboratory Director, Biospecimen Collection Programs Assistant Director, Anatomic

[Histonet] IHC Weekend Coverage

How many of you working in hospital-based pathology laboratories run IHC on weekends? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford

[Histonet] microscope stage controller spectra-tech

Hi Could someone help about a spectra-tech stage controller (model 0042-445 or else) : It works fine - I can manage the joystick (but with a little range problem) But I dont know how to use other functions. Any manual ? Any advice ? Best Richard

[Histonet] spectra-tech stage controller

Hi Could someone help about a spectra-tech stage controller (model 0042-445 or else) : It works fine - I can manage the joystick (biut with a little range problem) But I dont know how to use other functions. Any manual ? Any advice ? Best Richard

Re: [Histonet] Breast fixation

, and HER2 IHC results is inadequate fixation. Reference: Tong LC, Nelson N, Tsourigiannis J, et al.: The effect of prolonged fixation on the IHC evaluation of ER, PR, and HER2 expression in invasive breast cancer: A prospective study. Am J Surg Pathol 2011;35:545-552. Richard Richard W

[Histonet] foot pedal / microm hm355s microtome

connector, so may be linking some connectors with a simple wire could be ok ? Does anyone know about that connector ?) Other solution: does anyone have a spare Microm foot pedal ? Best regards Richard ___ Histonet mailing list Histonet

Re: [Histonet] CMV antibody clone

We use DAKO's (an Agilent Technologies Company) product number M0854 (clones DDG9 CCH2). Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT

Re: [Histonet] Ventana antibody - H pylori

Is the Ventana antibody polyclonal or monoclonal? Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax

Re: [Histonet] Histonet Digest, Vol 140, Issue 6 Specimen loss

Steve, I totally agree that 7% tissue loss is extremely high. Saying that when you have an experienced pathologist that looks at a specimen and only sees mucoid material he knows that it is most likely that the reagents in the processor are going to dissolve it. We have a very low rate of loss,

Re: [Histonet] IHC turnaround time

per month. Our IHC lab is staffed from 4:30 AM to 6 PM (or later depending on workload). We have 5 Leica Bond Max platforms; however, we still do some IHCs on the bench. We usually do 2-3 runs per day and an overnight run (except on Fridays) as well. Richard Richard W. Cartun, MS, PhD

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