Good morning Histonetters,
Well, the time has come to say farewell to Histonet and all the people it
represents. I've appreciated the invaluable information, humor and
encouragement that has been available here. I hope I've been able to help out
people now and then as well. I've never regret
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.e
We're looking for a reference lab that performs the above immunohistochemical
stain.
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
Sorry, I meant ARID-1a.
From: Sebree Linda A
Sent: Friday, October 23, 2015 9:17 AM
To: Histonet (Histonet@lists.utsouthwestern.edu)
Cc: Weisman Paul
Subject: Reference lab offering ARIDIA on FFPE tissue?
Hello Histonetters,
Anyone know of a reference lab offering this antibody?
Thanks
Hello Histonetters,
Anyone know of a reference lab offering this antibody?
Thanks,
Linda
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
__
Good afternoon,
Wondering if there are any reference labs offering this stain to perform on
FFPE human tissue.
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
_
Good afternoon,
Our lab is looking for a reference lab to send FFPE slides for this antibody.
The last place we sent them to was unsatisfactory.
Thank you in advance,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 537
Rabbit monoclonal, Richard.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: Cartun, Richard [mailto:richard.car...@hhchealth.org]
Sent: Thurs
We have excellent and very clean results with the VMS H. Pylori on our Ultras.
I can shoot you our protocol if you're interested.
Linda
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608
Brandy [mailto:bburn...@capecodhealth.org]
Sent: Thursday, July 02, 2015 12:07 PM
To: Sebree Linda A
Subject: RE: Ventana LCS (Liquid Cover Slip)
For IHC..We are rinsing them with dawn before running them down.
Brandy Burnett
Histotechnologist, QIHC(ASCP)
CCH Pathology/Histology
508-862-5267
bburn
We have 2 FTEs in IHC; myself as one of two people that started the lab 20+
years ago and 2 histotechs that rotate through on a weekly basis. Our monthly
totals currently run between 2400 and 3300. Our TAT policy is "in by noon, out
by 5pm" except for dual and triple stains (need to be on an i
I agree Jay. We have the same TAT policy and it's "kick ass" every single day
to achieve this with never enough time to cut controls, check out new lots,
bring on new antibodies, etc. Our staffing situation will need to change
pretty soon, especially as I look towards retirement :).
Linda A.
That's the reference I have always gone by since I first started doing IHC back
in 19..
Linda A. Sebree
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of
richardb...@charter.net [richardb...@char
Hi Craig,
As technologists, we of course QC all slides before they get to the
pathologist(s) but that is not documented unless we put a repeat order in the
case. Then we add a note saying why a test needs repeating, i.e. positive
control did not stain, patient tissue loss, etc. But this is al
Cleveland Clinic does it by IHC and I think also by IF.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern
Good morning,
We have a pathologist that has a case he wants sent out for HMF-1β (hepatocyte
necrosing factor-1β) . Are there any reference labs out there that offer this?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Mad
We use one from BD, Veronique, with good results.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Yes, 3 different antibody expressions per block.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-b
Good morning,
Does anyone know of a reference lab offering this IHC stain for human FFPE
tissue?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
___
Karla,
We try to run 1/2 of the required cases with known negative cases and the other
1/2 with known positive cases. In addition, all cases are run with a negative
reagent control in place of the antibody using the optimized protocol for the
antibody. These are your negative reagent controls
Does anyone know of a reference lab doing this antibody on human FFPE slides?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
___
Histonet mail
I fought this very same battle having been salaried for many years and
gradually working more and more OT. I worked solely with hourly employees and
was not a manager or supervisor. Everyone I worked with accrued OT pay but I
did not and I was usually working the most OT. I contacted our stat
This is by far my favorite!
Linda A. Sebree
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Weems, Joyce K.
[joyce.we...@emoryhealthcare.org]
Sent: Friday, October 10, 2014 11:35 AM
To: 'sarah.dys..
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: Jamal [mailto:j.rowa...@alborglaboratories.com]
Sent: Tuesday, September 30, 2014 5:15 AM
To: Sebree Linda A; 'Arrington, Karla A'; histonet@lists.utsouthwest
Hey Karla,
We use Vimentin as a positive reagent control, our negative control serum for
the negative reagent control and for the negative tissue control, the
pathologist reviews the antibody stained slide and OK's the internal negative
tissue elements as appropriately negative.
Hope that help
Well put Tim.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] O
Good morning Histonet,
We are looking for a reference lab to send FFPE slides to for the above
antibody(ies). We sent out for this test several years ago but of course at
that time our records were very rudimentary, i.e. log books, so difficult to
go back and search through. Hope someone kn
Sorry, I thought we were talking IHC/ISH not SS.
Linda A. Sebree
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Cooper, Brian
[bcoo...@chla.usc.edu]
Sent: Wednesday, September 17, 2014 2:36 PM
To:
We've had VMS instruments from day 1 and currently have 4 Ultras. The random
access capability is awesome and we can even get out EBER ISH slides the same
day if put on in the morning. Our HER2 dual ISH stains run overnight as they
are 14 hour protocols. Reproducibility among the 4 instrument
We do. I'll email Tuesday when I'm back at work.
Linda A. Sebree
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Rae Staskiewicz
[raest...@grics.net]
Sent: Friday, September 12, 2014 3:33 PM
To: His
Well said Joelle; we do pretty much the same.
Linda A. Sebree
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Joelle Weaver
[joellewea...@hotmail.com]
Sent: Friday, August 29, 2014 4:09 PM
To: Jb; h
I think your last question says it all; it ultimately is up to the director
although she/he better be able to explain to a CAP inspector why it was done
the way it was done. We generally follow the CAP guidelines with 10 - 20
+/10-20 - cases. We've also used the internal negative elements as b
Brandy,
We are completely paperless now so we created a code called "RPTIHC" that had
no billing charge associated with it. The pathologist then puts what
antibody/antibodies need repeating in the instruction box of the repeat code.
They also indicate the reason for the repeat(s). It populat
38 years and looking forward to retirement in a few years.
Linda A. Sebree
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Jamal
[j.rowa...@alborglaboratories.com]
Sent: Saturday, August 09, 2014 4:
f Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
From: Joelle Weaver [mailto:joellewea...@hotmail.com]
Sent: Monday, July 14, 2014 9:25 AM
To: Cartun, Richard; Sebree Linda A; histonet@lists.utsouthwestern.edu
Subject: RE:
Good morning,
I'd like to get some opinions on the storage of positive control slides for
HER2 neu. We currently keep a number of positive control slides in a -20
degree freezer as some antigens tend to lose reactivity at room temperature; ER
and PR come to mind. What has peoples' experiences
Hi Kari,
I'd suggest taking advantage of CAP proficiency testing surveys so as to
compare your lab's results to others.
Linda A. Sebree
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Delray Beach
Tasha,
We use the negative elements within our patient sample as our "negative tissue
control".
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-bou
We still run negative controls.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utso
This is our protocol for the Ultra; maybe it will help.
64" CC1, 32" incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4". This is
with UltraView DAB detection. We use tonsil as well however we validated with
HD, LN, GI, tonsil, etc.
Good luck!
Linda A. Sebree
University of Wisconsin Hospit
I was told "they're in the mail".
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.ut
Hi Tanya,
We have made the decision to continue running negative reagent control slides.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lis
That's what we use Anita and we purchase it from Cardinal as well.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
So Histonet land, our usual vendor for this antibody can no longer get this
clone. My question is: does anyone know who might hold the patent on this
clone or at least know any company selling the 6E10 clone?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laborato
We tried another vendor for ebar labels, FloPro Tek, easternlabsvc.com, but
they didn't hold up in xylene.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: hi
Sue,
I believe we used Amphyll at one point when we couldn't get Lysol IC. But that
could have been before we had our Ultras so used it on XT and Benchmark.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:
We also have Ultras Jim. We don't print out the run logs. IHC personnel
review the controls before they go to a pathologist in order to catch any
problems. The pathologists are supposed to review the controls associated with
each case they sign out. Our pathology report has a statement inclu
This is what we've done when we recently moved to control tissue on every slide
for IHC as well as Special Stains.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
We sent part of what we had to ProPath so if you need a reference lab to send
to, they offer this IHC test.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From:
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fa
Richard,
I wish we could eliminate them but not only do our negatives displaying some
non-specific staining, seems to be tissue dependent, but our in-house QA people
say we need to continue using them per the data sheets accompanying many of our
antibodies. We use Ventana UltraView DAB detecti
Elizabeth,
We get a rabbit monoclonal from Cell Marque, cat. # 117R, clone YR145 that we
are happy with.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: hi
Does anyone in Histoland know of a reference lab that offers this?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
___
Histonet mailing list
Hi
Hey Tom,
Although we have a higher workload than you, ~ 1500 slides/month, we offer same
day TAT with a cut-off of noon. There are a couple exceptions, i.e. triple
stains and HER2 dual ISH but otherwise, utilizing 4 Ventana Ultras, we have it
all out by 4 pm or slightly later. Our hours are 5
Well said Jean.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
We like our 4 Ventana Ultras for IHC, ISH and CISH.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histon
Hi Colleen,
We are just wrestling with this very issue and have decided to use:
Normal colon, i.e. staining present with all four antibodies
Colon carcinoma without the mutation, i.e. staining present with all
four antibodies
Colon carcinoma(s) with the mutation for each
Good morning everyone,
For those if you that are using Ventana's Inform HER2 dual ISH DNA Probe
Cocktail Assay; how are you reporting out results and do you put a disclaimer
of any sort in your reports?
Thanks for your responses,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/
Question everyone:
What are you all using for your positive tissue control when running the MSI
antibody panels, i.e. MLH-1, MSH-2, MSH-6, PMS-2? We're contemplating using
normal colon that all four antibodies should stain but do we also need to show
that colon having the mutation does not sta
Our docs here want to switch to TTF-1 and p40 for lung bxs rather than the
TTF-1 and p63 we're doing now. We will retain our p63 for other applications.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (6
ecca Anne [mailto:ra...@pitt.edu]
Sent: Thursday, August 01, 2013 2:40 PM
To: Sebree Linda A; histonet@lists.utsouthwestern.edu
Subject: RE: immunohistochemistry help please
Hi Linda,
I'm sorry - I should have been a little more clear. Once I realized it isn't
working, I did stop using the an
To test your deparaffinization Rebecca, I'd try staining a slide with something
cheaper and quicker, like just your counterstain to see if that's working.
Then go from there.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(
Good morning all,
One of our pathologists has requested this antibody and we don't have it in our
inventory. So I'm asking if there are any reference labs offering HNF-1B for
FFPE human tissue?
Thanks for any/all responses.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH L
We are required to keep all QA forms for 2 years.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...
Hey Tony,
We are using VMS antibodies (2 are manufactured by CellMarque) on our Ultras.
We've had to go with the Optiview detection to get satisfactory results. Let
me know if you'd like our protocols.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highlan
Do any of you do this? Do any referral labs offer this?
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
___
Histonet mailing list
Histonet@lis
Pat,
Right off the bat I can see 2 things that could speed things up for you. We
have Ultras and only dry our slides for 10 minutes at 60 d C. If tissues don't
stay on well, try some "Stay On" in your water bath; we're experimenting with
that now. Also, we spend way too much time ourselves s
Are there any reference labs offering this on FFPE specimens?
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
___
Histonet mailing list
Histonet@lists.
The dilution is listed on the bottle...I'm not at work but its something like
1:252.
Linda A. Sebree
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Amber McKenzie
[amber.mcken...@gastrodocs.net]
S
Rae,
We use Ventana's HP antibody with no problems.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-bou
Ashley,
Every new lot of antibody we get in is run with some of our control tissues and
compared to the current/previous lot staining. So we should be able to detect
a change. Our policy is that minor changes to protocols are OK but anything
major would require revalidation...we try not to go
Kimmie,
We use VMS' Hematoxylin II with no changes from how it has always stained.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet-boun...@lists.utsout
Hello Histonetters,
One of our pathologists is requesting Glutamine Synthetase and Heat Shock
Protein (HSP) 70 immunostains on a liver specimen. Our usual "go to" reference
labs do not perform these. Are there labs out there that have these tests
available? Even non-reference labs???
Linda
Jennifer,
We use p62/SQSTM1 from MBL (Medical & Biological Laboratories, Co., LTD), code
M162-3, as our Ubiquitin and alpha Synuclein from Invitrogen, #18-0215.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:
Looking for a reference lab offering the above.
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
___
Histonet mailing list
Histonet@lists.utsou
It depends upon what marker you're staining for. We fix our renal biopsies for
C4d in acetone for 10".
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-Original Message-
From: histonet
Deloris,
We recently had what we think was a contaminated dispenser of HP from VMS. It
had brown particulate matter scattered over the slide which the docs said was
not bugs. Received a new dispenser from VMS and have had no problem since.
Linda A. Sebree
University of Wisconsin Hospital &
ays been happy with their immunostainers.
Linda Sebree
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker,
Jr.
Sent: Monday, October 29, 2012 2:55 PM
To: CHRISTIE GOWAN; terri.br...@northside.com;
We sure like it Joe.
-Original Message-
From: Joe W. Walker, Jr. [mailto:joewal...@rrmc.org]
Sent: Monday, October 29, 2012 9:38 AM
To: Terri Brown; Sebree Linda A; Burton, Lynn; Gloria Tharp;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Dako vs Ventana IHC systems
Which
/director whether an extensive
validation is adequate.
Good luck with your decision,
Linda
-Original Message-
From: Kuhnla, Melissa [mailto:melissa.kuh...@chsli.org]
Sent: Monday, October 29, 2012 5:23 AM
To: Sebree Linda A; Glen Dawson; histonet
Subject: RE: [Histonet] IHC negative
I concur with Lynn; we've been dealing with Ventana 19 years.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burton, Lynn
Sent: Friday, October 26, 2012 3:47 PM
To: Gloria Tharp; histonet@lists.utsouthw
Hey Glen,
Don't know the answer to your specific question but on a related issue, our lab
has decided to maintain running negative controls due to the fact that the
manufacturer's data sheets for our IHC detection kits and a majority of our
antibodies require using +/- controls. The company in
In our opinion, yes.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Melissa Whitaker
Sent: Tuesday, October 23, 2012 9:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] validation runs
Hi e
H. Pylori by IHC; automated. I believe they're pretty much routinely ordered;
sure seems like it anyway!
Linda
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Wednesday, October 17, 2
We've always just billed for one stain so no difference for us; we'll continue
doing it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
llhartma...@comcast.net
Sent: Monday, October 01, 2012 6:25 PM
To:
We run a control(s) first before using it on patient samples. We
compare that slide to the same/similar control(s) run with the current
lot. Our director reviews both slide(s) and if comparable or better,
signs off on the new lot.
-Original Message-
From: histonet-boun...@lists.utsouthw
had discrepant comparison results.
Linda
-Original Message-
From: Kuhnla, Melissa [mailto:melissa.kuh...@chsli.org]
Sent: Wednesday, September 26, 2012 9:15 AM
To: Sebree Linda A; Joe Nocito; Vanessa Perez; Vickroy, Jim;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Changing fro
Joe,
We are abiding by the CAP 10/10 guidelines when at all possible and then
we compare our results with another method or same method but other lab,
i.e. reference or another clinical lab willing to trade slides with us.
The comparison part is where we are having issues as we, not me
personally,
I asked my VMS rep that question and she forwarded it to the higher ups
as they had not heard about this yet. Waiting for an answer but even if
VMS says we can omit them, our negative aren't all that clean always so
don't know if we will or not.
-Original Message-
From: histonet-boun...@l
I LOVE this Bernice!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice
Frederick
Sent: Thursday, June 28, 2012 11:15 AM
To: Fellow HistoNetters
Subject: [Histonet] A poem
I've had this for years- don
As far as I know you may only purchase MTM p16 through Ventana now...FDA
approved. BD sells p16 buts it's RUO.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Settembre, Dana
Sent: Tuesday, June 12, 2012 1
Follow the CAP guidelines for antibody validation; that's what we do.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wilson A
Sent: Wednesday, May 16, 2012 12:00 PM
To: histonet@lists.utsouthwestern.edu
S
Yes as well as the tissue usually run with these antibodies.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri
Johnson
Sent: Monday, May 14, 2012 10:15 AM
To: histonet@lists.utsouthwestern.edu
Subject: [
Yeah but they used to use canaries in mines to warn of toxic levels of
gases; having one in a histo lab might be a VERY good idea!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of William
Sent: Friday, May 1
I've always had at least one; makes the day more tolerable.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Behnaz
Sohrab
Sent: Friday, May 11, 2012 12:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [
Hello everyone,
I'm branching out into IMF antibodies and as such am starting to
validate these antibodies. Previously no comparison studies were done
that I know of. Now we're running frozen tissue slides manually with
our current set of antibodies simultaneously with new vendors'
antibodies be
Good morning Histonetters,
For purposes of validation, I'm looking for a reference lab that
performs IHC on human FFPE samples for several Alzheimer antibodies.
Specifically these antibodies are: PHF-1, CP13, anti-TDP-43 and LB 509
10.
I'd also be interested in any private/hospital/research labs
We're saving both for at least 2 years.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Rathborne, Toni
Sent: Thursday, April 12, 2012 9:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] New R
Peel off film here...works well.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Wednesday, April 04, 2012 11:50 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Slippery
Yup, that's what I'm looking for Histonetters.
Any info. is appreciated.
Linda
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