I haven't done so; usually manually process samples. But, lately, I have a lot
of specimens to process for TEM. Would appreciate you sharing your findings
with the list. I might like to think about automation at this stage.
Thanks!
Peggy
Peggy Sherwood
Research Specialist, Photopathology
To all:
I realize that this question has come up before, but what microwave do most
labs recommend for special staining? We have never had the need for one, but
now do. We would like an inexpensive one.
Thanks!
Peggy
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for
We recently purchased the Primera slide printer from Creative Waste Sotn's and
really like it.
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
I also agree. We've done this when the investigator requests being there to
help orient tissue. We just remove the cassette and let drain on paper towel.
Have never had a problem. (I wouldn't leave in liquid, hot paraffin, though).
Peggy Sherwood
Research Specialist, Photopathology
To all:
Would people please share their method for doing Congo Red staining? (We are
not interested in making up the reagents from powder form).
Thanks!
Peggy
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50
We don't clean them after each use, but we do put them in the oven (in a filter
over a container) so the wax melts. Every so often, not sure of the frequency,
we clean in CitriSolv (our solvent instead of Xylene).
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for
Just one minor change in the below mentioned protocol. Do you really do
water washes? I have never heard of that. We do buffer rinses. (I
personally used Cacodylate buffer, but people also use Phosphate buffer).
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for
We, of course, keep them separate from bases. We don't have large amounts, so
we keep them in cabinet under the hood. Do not keep in flammable cabinets.
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom
We have to have any container labelled (waste or reagent). We only date the
container when it is full and ready for pick up.
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
I love it! How true. I get frustrated as well. Enjoy your retirement!
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
I've never heard of it. Who sells this product? I tried googling, but could
not find the site.
Thanks!
Peggy
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839
We have a refurbished CV5030 and Autostainer XL and have had no issues with
them, or if so, minor ones that did not require contacting anyone.
Peggy
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
We are a research lab and usually the restrictions apply to flammables outside
the cabinet. I believe they don't want more than 100-150ml of any one
flammable. I don't think there is a restriction to what's stored inside
one--probably depends upon the size of the cabinet.
Peggy
Peggy
Best wishes, Sally. I hope you do fun things in retirement. I know you will
wake up and smell the coffee, just not at that ungodly hour of 3:45am!
Peggy
Peggy Sherwood
Research Specialist, Photopathology
Wellman Center for Photomedicine-EDR 214
50 Blossom Street
Boston, MA 02114
617-724-4839
I can't compete with the WalMart griddle(!), but EMS (Electron Microscopy
Sciences) sells the same para-trimmer for considerably less than Fisher ($827)
or Mercedes Medical ($724.50). EMS's price $350.00 (Cat. # 62850-10, 115VAC).
We have one and it works just fine.
Peggy
-Original
The one from EMS is called Wax Trimmer.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours
Sent: Thursday, March 29, 2012 12:56 PM
To: Stella Mireles; histonet@lists.utsouthwestern.edu
Subject:
Thanks, Tresa. I stand corrected. I did order ours from Ted Pella @ a year
ago for $289.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Goins, Tresa
Sent: Thursday, March 29, 2012 2:25 PM
To: 'Stella
) [mailto:tony.henw...@health.nsw.gov.au]
Sent: Tuesday, March 20, 2012 6:36 PM
To: Sherwood, Margaret; 'Troyer, Dean A.'; histonet@lists.utsouthwestern.edu
Subject: RE: MSDS regarding waste alcohol, xylene, etc
Good, though I can see a conflict of interest between the water authorities and
fire safety
I thought I would add my 2 cents to this discussion. Our hazardous waste is
regulated by the State and the MWRA (Mass Water Resource Association).
Basically, we cannot throw anything down the drains. Therefore, we collect our
alcohols, etc., label with hazardous waste labels, and store in
I concur. The problem is the dried picric acid. We used it so infrequently and
had a large bottle, that I had Safety dispose of it.
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA
To all:
We have been using the same slides for paraffins and frozens. We have no
trouble with paraffin-embedded tissue adhering to the slides through the whole
staining procedure. But our frozens (even 5um sections)have been falling off
during manual staining. The slides are (+) charged. We
To: histonet; Sherwood, Margaret
Subject: Re: [Histonet] slides for frozens
There is a known saying that goes: You get what you pay for.
René J.
--- On Thu, 2/2/12, Sherwood, Margaret msherw...@partners.org wrote:
From: Sherwood, Margaret msherw...@partners.org
Subject: Re: [Histonet
One of our investigators wants to take his frozen tissue (embedded in OCT) and
now fix in formalin for paraffin embedding. I assume he needs to wash out the
OCT (?phosphate buffer that formalin is prepared in) and then fix in 10%
formalin.
I would appreciate feedback from the listers.
Thanks!
We have the Leica Autostainer XL and companion coverslipper CV5030 (glass
coverslips) and like it a lot. We use the systems separately (space issue). We
have had no problems with either.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts
I would like to add an addendum to my prior email. We are a research lab and
don't have the volume of a clinical histology lab (run roughly 6 racks or 30
slides each/day).
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50
To all:
I know this has come up before, but where do most people buy their (+) control
slides for special stains?
Thanks!
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
(lab)
617-726-1206 (fax)
msherw...@partners.org
From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Wednesday, December 21, 2011 12:05 PM
To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret
Subject: Re: [Histonet] Control Slides
I never bought
Please share the link with all. I have the reference you mentioned.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
We are a research lab, but we use K2 (Karnovsky's fix) as well. Since
paraformaldehyde is hazardous (and a bit involved to make up), we simply buy the
glutaraldehyde and paraformaldehyde in sealed ampules and make it up in a 0.1M
cacodylate buffer. We also check the pH (7.4), not the osmolality.
I love Wait, Wait, Don't Tell Me! Interesting bit of trivia.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
To all who want to be removed from the Histonet List--you have to do it
yourself! Go to the website and follow the directions.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
I don't think you want to leave it in the processor for 2 hours. Even at the
embedding center, if someone can't come to help orient the tissue, we then take
the cassettes out of the paraffin station and leave on a paper towel. Have done
that for sometimes 2-3 days.
Peggy
Peggy Sherwood
Lab
That is precisely why we take the cassettes out of the molten paraffin
(embedding center) if they cannot be embedded right away. I felt they were being
cooked as well. We have had not problems sectioning cassettes that have
hardened and re-melted.
Peggy
Peggy Sherwood
Lab Associate,
We are a research lab and don't do a big volume of special stains. We just
received a project for 35 slides that need Von Kossa Stain.
I acid-washed some small glass staining dishes-no rack required (in the past, we
only had a few requests).
Can I run slides in plastic slide holders (24-place)
Thought of another question! What other vendors do people order special stain
kits? We have been using Poly Scientific, which is very good, but costly.
Thanks!
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50
We are a research path lab, but we follow the same guidelines. We keep 2 years
in the lab and 8 years in off-site storage.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
Ditto on the Leica CV5030.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org
-Original
We are a research group, but had paraffin blocks stored at room temperature for
20+ years. We finally made the decision to just keep 2 years in the lab and
store 5 years off-site. We trashed the rest.
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR
I don't know your specific protocol and what stains you are using (vendor), but
we switched a long time ago to CitriSolv, another xylene substitute and have had
not problems in our Leica Autostainer XL. We don't change the CitriSolv every
time and sometimes filter it.
Peggy Sherwood
Lab
Ditto. Sakura's Tissue-Tek
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherw...@partners.org
-Original
How is this site listed? I searched for Histo-Ville, but nothing came up.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206
We went the route of refurbished equipment. Got our stainer/coverslipper for
less than the price it would have been for one new.
Contact me off-line for specifics.
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
We use the marking pencils, exlusively, for our cassettes and StatLab marker
pens for our slides.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983
I would like to give input on this problem. We had a similar situation. The
investigators couldn't flush the fecal material before fixing-destroyed the
treated area of interest. After fixing, you couldn't flush the material out.
We ended up manually picking out the material before embedding
They sell there own supplies. We were just introduced to them by an independent
salesperson.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice mail)
617-726-6983
Ditto for Poly Scientific. We are a research pathology lab and have used Poly
Scientific exclusively for years. Their reagents are of excellent quality,
their tech support great and turnaround from ordering to delivery is timely.
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for
We have to have our processor paraffin picked up as hazardous waste, since it
has traces of CitriSolv in it.
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDR 214)
Massachusetts General Hospital
50 Blossom Street
Boston, MA 02114-2696
617-724-4839 (voice
Amanda,
We have a Microm HM550 cryostat and have used Brian J. Hurley (New England
Biomedical Services). He is an independent service engineer. He's very good.
In fact ThermoFisher uses him if a service visit is needed.
781-331-8642
617-774-7368 (cell)
Peggy
Peggy Sherwood
Lab Associate,
First, I would check with a Leica rep and see if he can help you. We have
ordered many replacement parts for our Autostainer XL (refurbished).
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of taylor alan
Linda,
I addressed this issue before. The smallest gauge needle that Leica supplies
for the CV5030 is a 21 gauge needle which we found to be too big (too much
mounting media came out and made a mess of the slides). We found a company on
the internet that sells re-usable metal needles in all
I don't believe that anyone responded to your inquiry. I used to make up a
Karnovsky's fixative (for TEM) (paraformaldehyde/glutaraldehyde) using the
powder form of paraformaldehyde. It is highly toxic. No one that I know of
does this anymore (or shouldn't). Now I buy the 16% solution of
I failed to mention that I make up my Karnovsky's and freeze aliquots. I have
never had a problem.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Suhyoung Jeong
Sent: Monday, June 06, 2011 8:39 AM
To:
We do the same.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
wanda.sm...@hcahealthcare.com
Sent: Monday, June 06, 2011 2:19 PM
To: jackie.flem...@allina.com; histonet@lists.utsouthwestern.edu
Subject:
Gayle,
I would be interested in the articles as well. I had a similar experience:
nerve samples from rats were being shipped to me in K2 (which I had previously
sent to them). I specifically said not to freeze the samples; they shipped them
on dry ice! I was just doing one micron sectioning,
Does anyone fix their frozen tissue first in formalin before embedding and
sectioning? I know one investigator in our lab did that previously, but was not
sure of the strength of formalin (I don't believe it was 10%). Another
investigator inquired about doing that.
I would appreciate hearing
Shirley,
I'm not sure if this is what you want, but we just ordered Hema-Diff Rapid
Differential Stain(a modified Giemsa/Romanowsky) from StatLabs. Consists of 3
solutions: Soltn 1: Fixative, Soltn 2: Xanthene Dye and Soltn 3: Thiazine Dye.
They sell it in gallon size and pint size. The gallon
Patsy,
I would appreciate receiving the flyers as well.
Peggy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Saturday, April 09, 2011 12:04 PM
To: 'Margaret Blount'; 'Pam Barker';
I'm curious what Giemsa stain method people are using for frozen sections? I
have a simple Wright-Giemsa stain that I use for blood smears (using a 1 min.
methanol fix, 30 second stain with Wright-Giemsa, 5 minute in Phosphate buffer
and then rinse in dH20). The investigator sent me a method
Actually, Bob, the method the investigator sent me was the Romanovsky Giemsa
that uses HEPES buffer. But it requires preparing all the reagents from scratch
and the HEPES alone is several steps. I would like to buy prepared reagents.
Can you buy a Giemsa kit for frozens?
Peggy
-Original
I stopped making up paraformaldehyde from the powder; too toxic. Another
colleague told me she buys it in a 16% solution, EM grade from EMS (10 x 10ml
vials). I started using that, along with the glutaraldehyde to prepare my
working solution.
Peggy
-Original Message-
From:
Allison,
We have a refurbished Leica CV5030 and do like it. However, I agree with
Beatrice, it took many hours of adjusting the drop size, and the ratio of
mounting media with sovlent, to solve the mounting media problem. We did end up
buying a smaller needle to dispense the mounting media and
The needles we ordered for our Leica CV5030:
The company is Intellispense-www.dispensinglink.com
They sell all sizes of re-usable (metal) luer-lok needles. We ordered the
following:
Cat. #9991258-523 gauge x 1/2 (pk of 12) - can't recall the price, very
cheap.
Peggy
Peggy Sherwood
My question is directed specifically to veterinary histologists or histologists
who also do a fair amount of animal processing. We are having a terrible time
processing pig fat. We had problems previously, but thought we had solved them.
This latest project (pig skin with a lot of fat attached)
The specimens can be large, but we trim so the thickness is less than 5mm.
-Original Message-
From: Marcum, Pamela A [mailto:pamar...@uams.edu]
Sent: Thursday, March 17, 2011 4:02 PM
To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu
Subject: RE: Re: [Histonet] Processing animal
We buy a cubitainer of 10% Buffered formalin and keep on the benchtop for
dispensing.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Chen, Shu-Cheng
Sent: Thursday, March 17, 2011 4:19 PM
To:
Just regular forceps.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett,
Jeanine (CDC/OID/NCEZID)
Sent: Tuesday, March 08, 2011 8:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]
We have a refurbished CV5030 coverslipper and we ordered directly from Leica.
If someone knows of anyone else who sells the parts/supplies, I would also be
interested.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
I would do a trial run on extra slides or non-valuable tissue?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mia Woodruff
Sent: Tuesday, February 22, 2011 10:33 PM
To: histonet@lists.utsouthwestern.edu
I routinely freeze Karnovsky's fixative (paraformaldehyde and glutaraldehyde
mix) in small aliquots. When you thaw, just vigorously shake to make sure all
is in solution. Have had no problems with fixation.
Peggy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
You do it yourself by simply unsubscribing. Follow the instructions on
Histonet.org. Then when you return, you subscribe again.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brian Branton
Sent:
We have small arteries that were FFPE and sectioned that should have been
decacified before processing. (The investigator did not indicate as such when
submitting them). Cutting was extremely difficult and the sections, as you can
imagine, had terrible knife marks and chatter. Of course, they
Could you explain in more detail the process, Liz?
Thanks!
Peggy
-Original Message-
From: Liz Chlipala [mailto:l...@premierlab.com]
Sent: Wednesday, December 08, 2010 1:47 PM
To: Sherwood, Margaret ; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Decalcification
Try
I want to thank the many people who responded to my inquiry re: decalcification
of embedded tissues. The responses were most helpful, especially the tips
people gave for dealing with the blocks.
Our pathology department has RDO and I will use that. I will let you know how
the sections come
We have this model as well and like it. We got the vacuum attachment. It's not
perfect, but does facilitate cleaning up OCT and tissue scraps.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell,
We have the Leica XL autostainer and CV 5030 coverslipper, both refurbished
units, and are very happy with them. We saved a lot of money by doing so. We
purchased both from Medical Equipment Source. We also purchased a Leica RM2255
automatic microtome as well.
Peggy
-Original
We also use tap water, but have a filter unit attached.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Avalos
Sent: Monday, November 15, 2010 12:51 PM
To: histonet@lists.utsouthwestern.edu
Ditto in Massachusetts. I work at MGH and we are striclty regulated by the MWRA
(Mass Water Resources Association) which monitors closely anything that goes
down the drain. They are especially concerned with mercury and the ppm are
very minute. Anything going down the drain can affect Boston
We always stored our unstained paraffin slides in slides boxes at -80 degrees.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Pop Elena
Sent: Wednesday, November 03, 2010 4:22 PM
To:
We have a (refurbished) Lecia Autostainer XL and really like it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kendall Neely
Sent: Friday, October 22, 2010 10:32 AM
To: histonet@lists.utsouthwestern.edu
We also love our Leica Autostainer XL (refurbished). Kevin, that is the way to
go if money is tight.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Wednesday, October 20, 2010 8:45 AM
The marking pencils work fine and we just ordered some marking pens from StatLab
which work great.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nita Searcy
Sent: Tuesday, October 19, 2010 2:22 PM
To:
Nita,
I would say you have quite a choice! For the longest time, we had so much
trouble with so many pens, we went to the pencil for the cassettes which are the
best. But we still needed pens for the slides; that's when we switched to
StatLab. We still use the pencil for the cassettes.
Peggy
Victor,
We are a research lab and don't generate near the amount of specimens that a
clinical lab would. We were happy to get an automatic stainer and coverslipper!
Peggy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I find it hard to believe as well. We have a refurbished unit and had to order
a number of accessories (wash pots as well)within the past year. I will contact
our sales rep and see what he says.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We use the low-profile microtome blades and love them. We actually found
Thermo-Fisher's brand of the low-profile to work just as well and they are less
expensive. We had problems with other manufacturers' low -profile blades that
had the oil on them. However we have several sample boxes and I
We clean our stainless steel base molds by soaking in solvent (i.e. citrisolv,
etc.) then wash, by hand, with soap and water.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L
Sent:
Masson Trichrome stains collagen and muscle.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret
Sent: Tuesday, August 17, 2010 5:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]
of Veterinary and Biomedical services
Box 2175
South Dakota State University
Brookings SD 57007
605-688-5638
Message: 20
Date: Thu, 12 Aug 2010 11:51:37 -0400
From: Sherwood, Margaret msherw...@partners.org
Subject: Re: [Histonet] metal base molds for paraffin embedding
To: histonet
To all:
We need to order metal base molds for paraffin embedding. We need a size
@45mm(L) X 32mm(W) X 11mm(D). (We have some, but have no idea where and when we
ordered them). I have looked in several catalogs but can only find a depth of
5mm. The depth is the important part.
If someone
I want to thank everyone who answered my inquiry into the stability of special
stains. And a special thanks to Rena Fail, for the link to the University of
Rochester's Special Stains manual. It was very helpful. You have answered my
questions.
Peggy
Peggy Sherwood
Lab Associate,
To All:
We have been experiencing problems sectioning mouse brain. The sectioning is
fine, but we have problems in the water bath. At 45 degrees C (peel-a-way
paraffin-Polysciences), the sections break apart but we don't get wrinkles.
With paraplast extra, we use 36 degrees C, but the sections
We used the metal trays as well for years. Switched to the styrofoam boxes and
they stay frozen longer. Just a thought.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Thursday,
We have a refurbished Leica Autostainer XL and are very happy with it. We are
a research lab, so our volume is not as much as a hospital histology lab, but we
get big projects every now and then and it has paid for itself.
If you are interested in purchasing refurbished equipment, please contact
We actually use a styrofoam box (used for shipping) and it stays cold longer
with very little melting. We keep a couple in the -20 freezer.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jay Lundgren
We have been using them (24x50) and have had no problems with broken glass
pieces. I do agree that they seem dirty.
Never contacted them about it.
Peggy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
We too save our containers; on several occasions we had the investigator
question the coding (usually their fault)! We keep ours for @a month
(overkill), and then empty the formalin into waste container for pickup and
discard the bottles.
-Original Message-
From:
By folders, I assume you mean the cardboard ones. Why not store the slides in
something else, i.e. plastic boxes?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer
Anderson
Sent: Tuesday, June
We leave ours on all the time; we do shut off the cryo center each night (it
frosts up otherwise).
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Tuesday, May 18, 2010 2:41 PM
To:
We are a research lab and occasionally do a few special stains (Masson
Trichrome, PAS, Picro-Sirius Red, Safranin O).
We usually purchase our stains ready made (or at least the components).
However, some of these stains are very expensive, namely the reagents for Masson
Trichrome. We just
I ditto Teri's suggestion. In a recent email to you, I mentioned the dangers of
using powdered paraformaldehyde. I have since stopped using it.
Peggy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
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