Re: [Histonet] Inquiry on Tissue Softening for Microtomy

My preference is cold water or Mollifex on faced blocks. Ammonia is definitely obnoxious (reminds me of wet nappies ) Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow,

Re: [Histonet] Histonet Digest, Vol 245, Issue 20

A few years ago there was an article on the Block - "What is Denatured Alcohol and What are the Implications For Histopathology?" that might be useful. The URL is

Re: [Histonet] Modified Davidson solution

I agree. A great book. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: John

Re: [Histonet] Modified Davidson

There are several formulations (some are probably typos), but this seems to be one commonly cited. Modified Davidson's Fixative: (Latendresse, J. R., Warbrittion, A. R., Jonassen, H., & Creasy, D. M. (2002). Fixation of testes and eyes using a modified Davidson's fluid: comparison with

Re: [Histonet] Processing artifact - delayed start

I would check the level of the formalin after it has been pumped into the retort. I will wait till the pics are posted Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s Hospital at Westmead (Retired) Adjunct Fellow, School of

Re: [Histonet] Faded H tissue section

I thought he may have reported this in Histologic. This trick certainly works. You can notice the nuclear clarity on GIT and to a lesser extent skin biopsies stained with PAS. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal Scientist, the Children’s

Re: [Histonet] Faded H tissue section

You could treat the decoverslipped section in 1% periodic acid (same as used in the PAS technique) for 30 minutes prior to H staining. This might improve the H staining. I believe Lee Luna suggested this but for the life of me, I can’t find the reference! Regards, Tony Henwood MSc, BAppSc,

Re: [Histonet] Formalin pH

Hew-Shue (1991) has described a useful pH indicator for working neutral buffered formalin solutions. Bromocresol purple, when added to formalin solutions, serves as an indicator of pH as well as, from a safety aspect, labelling the solution as formalin making redundant the dangerous “smell”

Re: [Histonet] shelf life of working antibody solutions in IHC

In Australia, we have evolved from Prescriptive accreditation (following the standards) to Risk-based accreditation where it is more important to assess the risk of a laboratory process rather than only meeting a list of rules, since following a list of rules is often not in the patient’s nor

Re: [Histonet] shelf life of working antibody solutions in IHC

Hi Gudrun, This should be useful ( I will send a copy separately) Henwood, A. F. (2023). Validation of nominally expired antibodies for immunohistochemistry. Biotechnic & Histochemistry, 98(2), 86-93. Regards, Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired) Principal

Re: [Histonet] Get the Short Term or Long Term Lab Staffing Coverage You Need

I agree Regards, Tony Henwood Sydney, Australia From: John Kiernan via Histonet Sent: Saturday, 3 June 2023 11:09 AM To: Melissa Owens; Jay Lundgren Cc: Tom

Re: [Histonet] Sudanblack B on FFPET

I would also let the saturated solution stand for a few days. Like Oil Red O, it probably needs time to “mature”. I would also use a frozen section of skin as a control. Regards, Tony Henwood Sydney, Australia From: Bob Richmond via Histonet Sent: