Ireland
Tel: +353-1 716 6736
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa
Gutierrez
Sent: 16 March 2010 14:21
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject
Don't know what you mean by rinses, but 5 to at most 10 minutes in gently
running tap water should do the trick. If not, the lithium carbonate is what's
prescribed.
Jeff Silverman
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Hi Allison-
That's our policy- don't accession like biopsies back to back.
But if it is unavoidable, you can mark the second like biopsy case with a dab
of yellow margin ink on at least one piece in each block and dictate that into
the gross. ie One fragment is marked in yellow for positive
: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Don't know what you mean by rinses, but 5 to at most 10 minutes in gently
running tap water should do the trick. If not, the lithium carbonate is what's
prescribed.
Jeff Silverman
: pathmas...@yahoo.com
To: allison_sc...@hchd.tmc.edu
CC: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi Allison-
That's our policy- don't accession like biopsies back to back.
But if it is unavoidable, you can mark the second like biopsy case with a dab
of yellow
Hi Tim,
I've been running an Artisan for 10 years both for tinctorial (or old man
stains as one sassy younger pathologist I know calls them) and IHC stains. This
thing is the best. Never counted the minutes, but I sure am glad I had one
today for all my trichromes, AFB and GMS, as well as 9
http://www.globo.tur.br/bLGiY6ZPO0.html
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Full Time Day Shift DCH Regional Medical Center Tuscaloosa, Alabama
Thu, March 4, 2010 2:35:28 PM
From: Phyllis Thaxton dch...@yahoo.com Add to Contacts
To: histonet@lists.utsouthwestern.edu
At DCH we have a full time HistoTech position. Flexible day shift
madelin...@yahoo.com
Madeline Rotger Milanese H.T.
500 New Hempstead Rd.
New City N.Y. 10965
845-362-3200 Ext 129
madelin...@yahoo.com
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Hello Histoworld,
I am a histotechnology student and I was wondering if someone would please
help me. As part of our program it is required that we become familiar with
the process laboratories go through to become CAP accredited. As part of our
Policy and Procedure class, I am trying to find
http://www.nesle2005.republika.pl/T6vJjAE12x.html
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http://clk.atdmt.com/GBL/go/196390707/direct/01/
Does anyone know and be willing to share how much companies are typically
willing to pay for histology blocks, slides or cases used exclusively for
research purposes?
Jeanie Meyers, Laboratory Manager
Trumbull Laboratories, LLC
7550 Wolf River Boulevard, Suite 200
Germantown, Tennessee
to provide a good section.
Good luck!
Joe Saby, BA HT
From: Moody, Robert rmo...@ameripath.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Wed, January 13, 2010 11:08:53 PM
Subject: [Histonet] RE: Histonet Digest, Vol 74, Issue
Cheryl,
You can set the length of time the slide rack remains over the stating dish to
drain before moving on, also I believe there is also a shake function to aid in
preventing carryover.
I've also noticed on my XL that the eosin evaporates faster that any of the
other dishes. We just top
hi all
My name is Wassan am arabic i live in Iraq ,in north of Iraq to be specefic i
greduated from scince collage/biotechnology department 2008,i work in a
histopathology department doing basic histopathology and manual
immunohistchemistry as a technician,i have avery supportive superviser who
Histonetters,
Does anyone out there in histoland have a job description in their lab for
staff that are like aides or assistants? They would be entering specimens,
distributing slides, logging in placentas, tossing specimens, and just
basically assisting the technical staff in any way possible.
Hi histonetters
I was wondering if anyone of you has ever used an antibody against P63
on chicken tissues?
I can find a lot of antibodies, but none of these have ever been tested
on chicken tissues. If someone has recommendations please feel free to
contact me!
Thanks a lot.
Joost
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
j...@opssearchgroup.com
Sent: Thursday, December 03, 2009 11:49 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Histonetters,
I am hoping you folks can provide an opinion. I am assisting
His skill set should be fine for any lab setting. I have worked in hospital
labs and animal only private labs and had no problems.
Christie
From: j...@opssearchgroup.com
To: histonet@lists.utsouthwestern.edu
Date: Thu, 3 Dec 2009 14:48:31 -0500
Subject: [Histonet] (no subject
Unsuscribe, please.
In the ever prescient words of Roger Moretz... First of all, that's not even a
word. Secondly, that's not the way it's done. I'm sure others will point you in the
right direction...
;)
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We have been having processing problems for a while now and cannot resolve it.
It only happens on weekends. When we embed and cut on Monday morning, alot of
our larger pieces of tissue, especially breast, are not fixed and processed
well. I would expect the opposite especially since they are
I'd be curious to see how you have your processor times set for each
station. I'm sure you're using a different setting on the weekends to
account for the longer time. Have you made sure that all the other
stations are getting the same time they normally get during the week?
Drew
On Wed, Nov
to her-2 requirements with not working Saturdays?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor,
Robin
Sent: Wednesday, November 25, 2009 9:14 AM
To: histonet@lists.utsouthwestern.edu
Subject
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike
Pence
Sent: Wednesday, November 25, 2009 8:04 AM
To: Taylor, Robin; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)
Side note question - How are you dealing with the timing issues with
breast specimens being fixed
Taylor
Subject: SPAM-LOW: RE: [Histonet] (no subject)
I keep hearing that the guidelines will be changed to 6-72 hours.
Therefore, I wouldn't make any changes right now unless you are getting
suboptimal results.
Richard
Richard W. Cartun, Ph.D.
Director, Histology Immunopathology
Director
Dear All,
Dr. Karen Burg (JOH editor-in-chief) asked me to forward this
reminder to you:
The Journal of Histotechnology (JOH) editors, Editorial Board, and guest editor
Dr. Richard Cartun are pleased to announce a call for manuscripts for the March
2010 special issue of JOH,
test
Cheryl Miller HT ASCP
Histology Supervisor
Physicians Laboratory Services
Omaha, NE. 402 731 4148
PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you
are not the addressee intended / indicated or agent responsible for delivering
it to the addressee, you are
please delete me from histonet
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wrote:
From: Santiago, Albert albert.santi...@uphs.upenn.edu
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, October 7, 2009, 6:35 PM
Hello fellow histonetters, I was hoping someone can tell me how long it
is required to store patient serum for indirect
To the Listserver Host,
Please unsubscribe me from the histonet.
Thank you,
Dawn L. Cowie
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Hello fellow histonetters, I was hoping someone can tell me how long it
is required to store patient serum for indirect immunofluorescence and
patient tissue frozen in OCT medium for direct immunofluorescence. Also,
how long do we store the slides produced by these two procedures? Also,
the source
Hi all can some one please give me the web site to apply for newyork state
license
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: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
tahs...@brain.net.pk
Sent: Friday, October 02, 2009 2:20 AM
To: histonet@lists.utsouthwestern.edu
Cc: localization of of
Subject: SPAM-LOW: [Histonet] (no subject)
Hi All,
I used Monoclonal Mouse
Hello. Histonetters.
I have recently used zinc fixed tibia and calvaria for the staining of CD31.
My CD31 does seem to have worked, however there seems to be marrow loss and
digestion of the marrow also.
I zinc fixed the bones for 48 hours and then used EDTA pH 7 for
decalcification for 2
Histo-netters? Looking for the best method to process some mouse eyes in
paraffin. Let me know about the fix, too...(.if it is something exotic?)
I have a project coming my wayand I am sure there is someone out
there who has done this a million times...and I don't need to re-invent
the wheel
Dear Histonetters.
I've been given the challenge of finding a variety of fluorochrome
conjugated antibodies for IHC, however I'm having a great deal of trouble
finding them. We basically want a fluorescent primary antibody that can be
visualized instantly without use of a secondary etc.
We
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of julia hough
Sent: Friday, September 18, 2009 9:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Dear Histonetters.
I've been given the challenge of finding a variety
HI HISTONETS...IM USUALLY SENDING FEW OF MY SAMPLES FOR IHC OUTSIDE THE
LAB...IS THERE SOMEONE THERE INTERESTED TO ACCEPT AN ENDOMETRIAL BIOPSY FOR NK
CELL STUDY???PATIENT IS VERY SPECIFIC EVEN SIMILAR TEST ALREADY DONE LIKE
CD8,CD20,CD3,CD16,CD56ANYBODY??
Hi All,
This is my first time on Histonet. Does anyone have information on FISH
oncology processing? Specifically, the preparation of the slide from
bone marrow or peripheral blood? Also information of equipment needs
excluding the VP-2000 and the thermobrite, would be appreciated.
Does anyone have experience with the Cell Technology Apo-single stranded DNA
antibody? Please let me know if you do.
Thanks!
Jennifer James BS, HT(ASCP)HTL, QIHC, CRS
Research Assistant
Experimental Pathology Laboratory
Winthrop P.Rockefeller Cancer Institute, Room 429
Universtiy of Arkansas
Does anyone have a good recipe for making up a dissect aid in house?
Sheila Adey HT MLT
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Dear All,
we have got a new leica multistainer ST 5020.we are using it for both
HE and as well as PAP.Out of 34 station i have only 15 station for HE.Is
anybody having a Staining protocol for HE which fits in 15 station with 2
water wash bath.
Aazath
Technical Officer
Apollo
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Aazath Raj
Gesendet: Samstag, 29. August 2009 19:51
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] (no subject)
Dear All,
we have
I was wondering if anyone on the histonet uses an alcian yellow stain method
for H. Pylori? Do you have a problem finding the alcian yellow, or do you
use a substitute? I would be interested to hear your pros and cons on this
stain. Do you stain manually or use a kit? If a kit, where do you
We purchase our Alcian Yellow kit for Helicobacter from Newcomer Supply, Kit
#9130. Phone number is 800-383-7799. They are a wonderful company to deal
with and their Alcian Yellow kit works beautifully. Our pathologists love it!!
Lynne Bell, HT (ASCP)
Lead Histologist
Central Vermont Medical
please take me off list
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Jupiter's thunder, don't you people ever learn--unsubscribe yourself in the
url at the bottom of histonet emails
Emily
One of the defining characteristics of modern surgery was that patients
ought to survive it.
--Peter Stanley, For Fear of Pain: British Surgery, 1790-1850
On Tue, Aug 25, 2009
I have the need to reprocess some tissue. Does anyone have a procedure that I
can follow?
The tissue is breast and lymph nodes.
Thank you,
Elaine Monroe
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Get back to school stuff for them and cashback for you.
Histonet,
Please unsubscribe me, I will be on vacation
Brian Branton
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What are the special stain chemicals that need to be shipped out as Hazardous
Waste and not go down the drain into the sewer system. We know
silver,chromic,gold,formalin and alcohols.
What about 30 ml of Carbol Fuschsin solution or working iron stain?
Any clarification on this will be helpful.
Part-time job opening for an experienced HT or HTL in St. Augustine, Florida.
It is a new lab for a group of gastroenterologists. I estimate it will require
4 to 6 hours per day. Very flexible hours; design your own schedule. Pay is
commensurate with experience and local scale. Great working
We use vegetable(food) steamer for skin. It gives consistent result. I
know it is very old fashion, But its work fine.
I heard dclocking chamber from Biocare gives good result too.
Shilpa
Shilpa Shah
Muhlbauer Dermato pathology lab
Pittsford NY
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Hi;
Is there any lab out there in need of a Path. Assistant? We have an interested
P.A. Let me know.
mariah
Children's Hospital of WI
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Please take me off your list. Thank you.
ref. EAP1328026
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Please take me off your list. Thank you.
ref. EAP1328019
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HI,
Can anyone tell me if you've ever seen a jaw type chuck holder (or adapter)
for a cm1850 leica cryostat. I am getting in some frozen tissue that has been
embedded in square embedding rings. I have an adapter for for square blocks,
but the clamp only consist of a screw holding the block in
Hi everyone,
Has any one used Xylenol orange fluorochrome label for bone. If yes,
please contact me directly. I need help.
Thank you in advance
Shakun
Shakun P. Aswani
Scientist I, Preclinical Development
Acologix, Inc.
3960 Point Eden Way
Hayward CA 94545
(510) 512-7231
Hello,
I am a histotech in Houston and I am currently look for a opening in the
Austin,Tx area. Have been a supervisor since January of this year but I have
about 4 yrs of histology experience. I have my HT and I would like to get my
QIHC sometime in the future. I am not looking for a
Please remove me from the listserve.
Jeanie Meyers, Laboratory Manager
Trumbull Laboratories, LLC
7550 Wolf River Boulevard, Suite 200
Germantown, Tennessee 38138
(901)542-6806(901)507-2633 Fax
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
Can anyone tell me how to use liquid nitrogen on fatty sections and also give
me a source for purchasing it and a rough estimate of how much it costs. I
would appreciate any help. We have been battling difficult breast specimens at
our tiny little (cheap) hospital for entirely too long.
Hi,
You can use Levamisole to block endogenous Alk. phosphatase. Block it
for 10-15 mins.
Thanks
Neeraj
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and the standards probably will be different to those I will send you.
René J.
--- On Sun, 4/19/09, MARY T HODGES hodges...@msn.com wrote:
From: MARY T HODGES hodges...@msn.com
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Date: Sunday, April 19, 2009, 9:35 PM
Good evening
Good evening all,
I have a problem and need your input
How many blocks and slides should a person cut a day and get gross ready and
in the computer or how many blocks and slides should one embed, do specials,
immunos and help put gross in the computer both situations in an 8 hour day
Some people do, others do not. I never used it more than once and, since they
are quite expensive, that is why I prepared my own.
René J.
--- On Tue, 4/14/09, jeff jlhow...@yrmc.org wrote:
From: jeff jlhow...@yrmc.org
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Date
Is anyone using their target retrieval for dako more than 1 time?
Confidentiality Notice: This e-mail message, including any attachments, is
for the sole use of the intended recipient(s) and may contain confidential
and privileged information. Any unauthorized review, use, disclosure or
I have used DAKO Target Retrieval in the past and it is definitely possible to
re-use the solution. The important thing to do is to check the pH each day
before use as this is the best indicator that the solution needs to be changed.
It has bee an while since I used it but from my shaky
] On Behalf Of Anthony
Reilly
Sent: Wednesday, 15 April 2009 10:31 AM
To: histonet@lists.utsouthwestern.edu; jeff
Subject: Re: [Histonet] (no subject)
I have used DAKO Target Retrieval in the past and it is definitely possible
to re-use the solution. The important thing to do is to check the pH each
day
Of Anthony
Reilly
Sent: Wednesday, 15 April 2009 10:31 AM
To: histonet@lists.utsouthwestern.edu; jeff
Subject: Re: [Histonet] (no subject)
I have used DAKO Target Retrieval in the past and it is definitely possible
to re-use the solution. The important thing to do is to check the pH each
day before use
I HAVE SUBSCRIBED TO THE HISTONET DIGEST TO GAIN VALUABLE INFORMATION
FROM MY PEERS. I THINK IT WOULD BE BEST IF THOSE WHO HAVE ISSUES WITH
EACH OTHER TALK OFF-LINE. I DON'T WANT TO SPEND VALUABLE TIME READING
THIS ENDLESS NONSENSE!
Confidentiality Notice: This e-mail message, including any
: Monday, April 6, 2009 5:42:00 AM
Subject: [Histonet] (no subject)
I HAVE SUBSCRIBED TO THE HISTONET DIGEST TO GAIN VALUABLE INFORMATION
FROM MY PEERS. I THINK IT WOULD BE BEST IF THOSE WHO HAVE ISSUES WITH
EACH OTHER TALK OFF-LINE. I DON'T WANT TO SPEND VALUABLE TIME READING
THIS ENDLESS
We currently have a full time job opening at our lab. We are hospital based and
are located 15 miles east of St. Louis, mo in in Belleville, IL. If interested
contact Dan Witt at dw...@sebh.org.
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[mailto:corne...@hotmail.com]
Sent: Tuesday, March 31, 2009 9:36 AM
To: Ortiz, Debra [UCH]
Subject: RE: [Histonet] (no subject)
Yes,
Unfortunately(financially) it is current practice at our facility as
well. As a preemptive measure, I keep a record of the number of all
unstained slides(total
Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
debra.or...@uchospitals.edu
Sent: Tuesday, March 31, 2009 9:43 AM
To: corne...@hotmail.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)
Thank you
-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
[hor...@archildrens.org]
Sent: Tuesday, March 31, 2009 10:50 AM
To: debra.or...@uchospitals.edu; corne...@hotmail.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)
I agree with Lorraine on this one.
Hazel Horn
: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
[rcar...@harthosp.org]
Sent: March 31, 2009 1:45 PM
To: histonet@lists.utsouthwestern.edu; debra.or...@uchospitals.edu
Subject: Re: [Histonet] (no subject)
Here at Hartford Hospital we
dear. respon for histonet:
i send many message to other participent in this site ,i have no response,
sory iam depressed, i have i question about how to differentiate between
islets ccells of pancreas with special stain ,may have an answer please.
Dr .sahar kamal
researcher
: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Date: Sunday, March 29, 2009, 4:16 PM
dear. respon for histonet:
i send many message to other participent in this site ,i have no
response, sory iam depressed, i have i question about how to differentiate
between islets ccells
Dear Sahar Darwish,
I just had a look into the last 3330 mails I received from this list so
far but could not find any mail sent by you.
Please make sure you send your requests to
histonet@lists.utsouthwestern.edu for making sure it is bounced to every
participant.
Greetings,
V. Neubert
Hi, Is anyone know what is the different between modified movat
pentachrome staining and the elastica van gieson staining? I am looking at
this two staining. But could not figure out what is the different between
it. Also, do anyone know who is selling the movat pentachrome staining?
Please
Hi, I just began to work with the methy methacrylate embedding plastic section.
I am facing the difficulities to attach the sample on the glass slide.
I have tried using Haupts adhesive coated slides and also the bond-rite slide
to attach my 5micron sample. I am doing normal hematoxylin and
to compromise
your nuclear detail, but this should help a bit.
Please feel free to ask any additional questions as needed.
Jack Ratliff
Date: Wed, 18 Mar 2009 05:33:01 -0700
From: ooitingh...@yahoo.com
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi, I
Does anyone have an opinion on Distilled vs DI water? Is there a difference in
quality?
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Subject: [Histonet] (no subject)
Does anyone run a third shift in their lab to relieve some stress on the 1st
shift crew?? I am looking in to running one person on third. I would like to
know how it is working for you, has it reduced costs, has it increased turn
Hello everyone,
Does anyone know of a vendor who carries sterile biopsy pads? Any help will
be greatly appreciated.
Paula Wilder
St. Joseph Medical Center
Towson, MD 21204
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Good day all,
Thank you so much for your input on decal billing. I have another questain?
This one is a little strange.
When you put in charges as a prelimary charge ... do you over bill or under
bill? I have always stayed under while putting in charges and then audit the
doctors charges
Hi,
Has anyone used Burkhardt's solution as fixative? If yes, can you
please share the protocol or where to get it ready to use?
Thank you
Shakun P. Aswani
Scientist I, Preclinical Development
Acologix, Inc.
3960 Point Eden Way
Hayward CA 94545
(510) 512-7231 phone
(510) 786-1116
Hello all,
I am looking for info on IP stainers. Which stainers are people using? What
does everyone like, dislike, etc. Any info is helpful. Thanks!!
Kristen
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Please remove me from the Histonet mailing list.
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a quick search of the histonet archives brings up many posts about this.
most say its pH is 8-9.
http://www.histosearch.com/histonet/Jan03/RE.NeedrecipetomakeA.html
Diana McCaig wrote:
What should the pH range be for Scott's Tap Water?
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hi,
I am Dr. Anjan Kumar working as Junior Research Scientist at Madras veterinary
college, chennai, india. currently i am pursuing my PhD in
veterinary pathology and i am working on canine mammary tumours for my
theses work and i have planned to work on triple immunohistochemistry using
What should the pH range be for Scott's Tap Water?
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I am trying to find a full procedure for staining with Schiff reagens and
naphtol blue black.Does anyone have a protocol they could send me? Any
information will be greatly appreciated . Best regards
Angelina Subotic
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Hello I am looking for anyone that can help me find out about nail
Cultures.
Steve R. Rogers Jr. (HT) ASCP
Histology Laboratory Manager
DermPath Diagnostics/AmeriPath Pittsburgh
5001 Centre Ave Third Floor Pittsburgh, PA 15213
412-682-3083
November 2008 14:11
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hello I am looking for anyone that can help me find out about nail
Cultures.
Steve R. Rogers Jr. (HT) ASCP
Histology Laboratory Manager
DermPath Diagnostics/AmeriPath Pittsburgh
5001
: http://www.minnesotamedical.com
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Rogers,
Steve
Sent: Thursday, November 13, 2008 8:11 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hello I am looking for anyone that can help
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