Hello Everyone,
We need to bring in EDTA as a decalcifier for bone biopsy specimens with
metastatic cancer that would receive molecular testing.
Does anyone have advice they could share on a validation?
Thank you,
Stacy
Stacy McLaughlin, HT(ASCP) QLScm
Histology Supervisor
Cooley Dickinson
We didn't compare EDTA with formic acid. But heard that formic acid also
can do many IHC and ISH, if not all the antibodies and all probes. I always
have problem of bone falling off, no matter what kind of slides. Thinking
both PFA and EDTA affect on this issue. So trying frozen tape unfixed,
6:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] EDTA decalcification tissue issues
This email is from outside HHC. BE CAREFUL when opening attachments or links
from unknown senders.
Good Morning,
Recently, our lab has been working on validating an EDTA method of
decalcificatio
Good Morning,
Recently, our lab has been working on validating an EDTA method of
decalcification. When we ran the IHC's on the decalcified bone block, the
majority of the tissue lifted off the slide. We use the Leica Bonds for our
IHC staining. Does anyone else have a hard time getting EDTA
I am asking this question for our Histology Lab. They are being asked about
using EDTA as a decal solution for bone and wondered if anyone else is using
this?Is this available as a ready to use? Or do you have to make it up?
What vendors are you using and could you provide a procedure
Hello,
We make our own formic acid EDTA for a slow decal solution. Is this what you
are looking for?
Thanks
Histologistics
Hans B Snyder
508.308.7800
h...@histologistics.com
On Dec 1, 2014, at 14:20, Martha Ward-Pathology mw...@wakehealth.edu wrote:
I am asking this question for our
antibodies.
Gudrun
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Teri
Johnson
Gesendet: Freitag, 16. August 2013 19:54
An: 'cfors...@umn.edu'
Cc: histonet@lists.utsouthwestern.edu
Betreff: [Histonet
...@umn.edu'
Cc: histonet@lists.utsouthwestern.edu
Subject: [Histonet] EDTA decal
Hi Colleen,
I would say it's unusual, but not completely impossible that EDTA has
interfered with your IHC. We had that problem with demonstrating
B-galactosidase in mouse bones. If we decalcified it in EDTA after whole
Hi Colleen,
I would say it's unusual, but not completely impossible that EDTA has
interfered with your IHC. We had that problem with demonstrating
B-galactosidase in mouse bones. If we decalcified it in EDTA after whole mount
staining in X-gal, the blue staining was removed. But if we
Dear List members
Greetings
I would like to ask you some questions about the use of EDTA as demineratizing
agent.
I have to do histology and histochemistry (including metallothioneins) in fish
larvae preserved in Bouins solution.
1) First of all I would like to know what is the best formula
Subject: [Histonet] EDTA
Dear List members
Greetings
I would like to ask you some questions about the use of EDTA as
demineratizing agent.
I have to do histology and histochemistry (including metallothioneins) in
fish larvae preserved in Bouins solution.
How long do you fix in Bouins
Why don't you prepare it in your lab? By doing so you will cut costs and assure
a consistent decal solution.
René J.
--- On Fri, 7/22/11, pam plumlee paw...@yahoo.com wrote:
From: pam plumlee paw...@yahoo.com
Subject: [Histonet] EDTA 14%
To: histonet@lists.utsouthwestern.edu histonet
Dear Histonetters,
We would like to decalcify some mouse bones in EDTA pH 7 which have been
received by our lab already fixed directly in 70% ethanol rather than in
formalin or paraformaldehyde.
How would you recommend preparing these bones for decalcification e.g.
whether to post-fix in
...@sheffield.ac.uk
Subject: [Histonet] EDTA decalcification of bones which have been fixed in 70%
ethanol
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, June 22, 2011, 7:55 AM
Dear Histonetters,
We would like to decalcify some mouse bones in EDTA pH 7 which have been
received by our lab
, this appears
to be the case for some antibodies. Unfortunately as with most things in
histology, there is always exceptions to rules of thumb!
--- On Fri, 2/19/10, Rene J Buesa rjbu...@yahoo.com wrote:
From: Rene J Buesa rjbu...@yahoo.com
Subject: Re: [Histonet] EDTA
To: 'histonet
I am looking into the various decals on the market and have found one that in
addition to formic acid, has EDTA in the mix. I have never worked with EDTA so
would appre ciate any help in your comments on the use of EDTA in
decalcification methods for bone marrow and routine specimens.
Thank
decalcification that will be
completely suitable for IHC studies.
René J.
--- On Fri, 2/19/10, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote:
From: Webb, Dorothy L dorothy.l.w...@healthpartners.com
Subject: [Histonet] EDTA
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Hi Patti,
Years ago, I used a EDTA Decal Solution which was recommended by Dr. Jaffee, a
Hematopathologist at NIH. This formula was particularly great for BM Core
BX's. It is much gentler on the tissue, and is less likely to harm the
cellular integrity. Dr. Todd Barry also prefers the use of
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