[Histonet] FISH testing

Does anyone use an automated staining platform to perform FISH testing? If so which one and are all the steps automated or do you have to perform any steps manually/ on another platform for any reason? ___ Histonet mailing list Histonet@lists.utsouthwe

Re: [Histonet] FISH for breast core (Her2)

Hello Taganrog, If you are having issues with high autoflorescence due to prolonged formalin fixation (7 days), it might help to do a few minutes extra protease digestion (5+ minutes) during tissue pretreatment. Alternately or in conjunction, I would suggest re-applying HER2 probe to the same sl

Re: [Histonet] FISH for breast core (Her2)

Maxim, this is a link to the cIQc assessment page http://cpqa.ca/main/?page_id=24 If you look at the HER2 ISH assessments you will see numerous protocols used by laboratories. To my knowledge the protocols work on both biopsy and resection tissue. John www.ciqc.ca ‐‐‐ Original Message ‐‐

[Histonet] FISH for breast core (Her2)

Dear colleagues! Can anyone help? We are very appreciatated any suggestions, tips and hacks for Her2 FISH onto breast trepine fixed in 10% NBF. It was here for more than 7 days, then processed as usual into wax. IHC for Her2 has value as 2+. Sincerely, Maxim Peshkov, Russia, Taganrog. _

[Histonet] FISH on BondMax

Dear all! I would appreciate input on the FISH-function of the BondMax from Leica. Is this feature reliable and better/as good as manual staining? Would anyone recommend this instrument for FISH staining? Thank you Lang Gudrun ___ Histonet mail

Re: [Histonet] FISH question

richt- Von: Whitaker, Bonnie [mailto:bonnie.whita...@osumc.edu] Gesendet: Dienstag, 19. Februar 2019 18:54 An: 'Mark Tarango'; Gudrun Lang Betreff: RE: [Histonet] FISH question I know a few years ago, we ran into the same issue, and the problem actually was with the slides. We wer

Re: [Histonet] FISH question

Hi Gudrun, Are you sure you have digested long enough with pepsin? If the tissue is not well digested you will see background. We use sodium thiocyanate for pretreatment reagent, not citric buffer. These are my first thoughts. Mark On Tue, Feb 19, 2019 at 9:18 AM Gudrun Lang via Histonet < hi

[Histonet] FISH question

Dear histonetters! I have difficulties with my FISH preparation on FFPET. I struggle with massive background. It looks like a thick fluorescent film. The signals can't be seen because of the background. Even the nuclei are hard to see. The background is within the tissue but also surrounds it. T

[Histonet] FISH on paraffin in pediatrics?

Hello Histo world, Is there anyone out there who is doing FISH on FFPE tissue in pediatric facilities? Seems most of the research is going in the direction of adults and I would like to incorporate some of these new testing procedures in our lab. Cytogenetics is getting busier and busier, His

[Histonet] Fish Skin Histology

Hello Histonet, I am looking for advice on the best method to prepare fish skin and eyes for sectioning. If you have had experience with this in the past, I'd love to hear what worked for you. Thanks in advance! Anna Anna Coffey, MS, HTL(ASCP)CM Histotechnologist Center for Advanced Preclinica

RE: [Histonet] fish eggs: processing and fixation

Sadly we don't have the capacity to do frozen sections :( Christy -Original Message- From: Diane G. Miller [mailto:dgmil...@coho.net] Sent: October 23, 2014 11:56 AM To: Thompson, Christy Subject: Re: [Histonet] fish eggs: processing and fixation Have you tried doing frozen sec

Re: [Histonet] fish eggs: processing and fixation

Usually fish eggs are only processed by lightly salting and pressing. I've never tried sectioning them. Just serve with toast points and lemon wedges, maybe a little minced onion, and of course dry champagne! Sincerely?

[Histonet] fish eggs: processing and fixation

Hi all, I'm currently working on some unfertilized fish eggs. They were fixed in 10% NBF and processed in a vacuum processor with paraffin. The eggs are extremely hard to section and most of the yolk goes AWOL on the way to the water bath. I did do a search here for some help and a few things were

[Histonet] FISH Applications Specialist

Good morning! We are searching for an Applications Specialist to join a world leading cancer diagnostics company. The position will focus on FISH and cytogenetics and will be based in the IL area. The position will provide internal support as well as provide customer support for the West Coast.

[Histonet] FiSH

Does anyone use PersonalizeDx for FiSH specimens? We recently started using them and I have some questions. Juliana Montero, HTL Lead Histotechnologist USMD Diagnostics Services, LLC Pathology Lab 900 Airport Fwy, Hurst, TX 76054 Office: (817) 576-0590 Fax: (

[Histonet] FISH technique and NBF as fixative

I need to do FISH with the universal bacterial probe EUB338. I am not too familiar with FISH. Can we fix the sample with NBF or is 4% paraformaldehyde better? The samples are acellular dermal matrix. A quick lit search shows people using 4% paraformaldehyde, but the histologist that will be em

RE: [Histonet] FISH enumeration

...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Saturday, September 07, 2013 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FISH enumeration For any laboratories out there who perform in house FISH procedures, if

AW: [Histonet] FISH enumeration

...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von joelle weaver Gesendet: Samstag, 07. September 2013 16:22 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH enumeration For any laboratories out there who perform in house FISH procedures, if

[Histonet] FISH enumeration

For any laboratories out there who perform in house FISH procedures, if you could share what personnel are responsible for doing the signal enumeration & scoring? It would be helpful if you could describe the personnel's training and certification, as well as an approximation of FTE's needed w

[Histonet] FISH signals

Hi, how the FISH signals in FFPE tissue can be strenghten? by standard procedure only weak signals for EGFR can be obtained, so far I tried different time in Vysis protease 1 (45, 60, 70 min), for other probes 70 min works quite well on our FFPE, but not for EGFR? what other modifications can

Re: [Histonet] FISH baking and dewaxing

> An: histonet@lists.utsouthwestern.edu > Betreff: [Histonet] FISH baking and dewaxing > > Can someone please explain why paraffin-embedded slides for FISH need to be > baked for so long and have extensive dewaxing in xylenes and subsequent > alcohol pretreatment? > Our Molecular group b

AW: [Histonet] FISH baking and dewaxing

Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Houston, Ronald Gesendet: Donnerstag, 14. Februar 2013 20:08 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH baking and dewaxing Can someone please explain

Re: [Histonet] FISH baking and dewaxing

Hi Ronnie, FISH pretreatment can be harsh and that might explain the long baking (don't want the tissue to fall off). On the other hand, I've put a slide in the oven for 5 minutes and had the tissue stay on in a rush. I think the dewaxing can be shortened without affecting the FISH signals. Our

[Histonet] FISH baking and dewaxing

Can someone please explain why paraffin-embedded slides for FISH need to be baked for so long and have extensive dewaxing in xylenes and subsequent alcohol pretreatment? Our Molecular group bake for >1 hr at 65C, and then treat sections in 3 changes of xylene 15 min each, and 100% ethanol 15 min

AW: [Histonet] FISH

-Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von wassan alkadhumi Gesendet: Montag, 23. Mai 2011 21:24 An: h n Betreff: [Histonet] FISH Dear members   We just started doing FISH using HER2 FISH pharma Dx Dako kit, Code

[Histonet] FISH

Dear members   We just started doing FISH using HER2 FISH pharma Dx Dako kit, Code number K5331 on malignant breast tissues.  I was wondering what is the success ratio or percentage for say a one run?? my hospital is the first in our region to start FISH   and I am the first to run it. I have no

[Histonet] FISH

Hi everyone, I was wondering if anyone would like to share their protocol for FISH on PFA fixed tissues? Any help would be hugely appreciated. Thanks so much. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu

AW: [Histonet] FISH procedures

tonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Mimmo Pozzuoli Gesendet: Samstag, 25. September 2010 17:05 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH procedures A question for people working in urology and other types of hist

[Histonet] FISH procedures

A question for people working in urology and other types of histology labs... Do histotechnicians perform FISH procedures in your lab or is it required to have an MT on board to do such tests? I have heard of ISH being done in histology settings and imagine that the automation of them has lent a p

[Histonet] FISH Validation

Hello All in Histoland,   I am once again requesting your expertise in the Molecular FISH world of validation.  I am treading on unfamiliar territory here.    I am very familiar with validation for histology and IHC, but not FISH.  I was requested to gather information regarding how many cases

Re: [Histonet] FISH PROBES

IDLabs works great for us. Cambio is referenced a lot in the literature, but their Y-chromosome porcine probes didn't work for us. --On Wednesday, February 24, 2010 2:42 PM -0500 dcoj...@tampabay.rr.com wrote: Does anyone know

[Histonet] FISH PROBES

Does anyone know of a reliable company to purchase FISH probes from (other than Abbott)? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Fish Gills

Cammi - Our lab processes thousands of fish specimens yearly. Fish of all ages. If the fish is initially fixed in Davidson's there should be no need for decalcification. Older, larger fish, just like mammals, may take further softening. If you have specimens already processed that don't cu

AW: [Histonet] FISH Qualifications/Training

-boun...@lists.utsouthwestern.edu] Im Auftrag von Remy Lopez Gesendet: Freitag, 22. Jänner 2010 17:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH Qualifications/Training Good Morning! Does anyone know what are the requirements or qualifications for people to be able to read HER 2

Re: [Histonet] FISH Qualifications/Training

Usually counting is done by a trained histotechnologist. Some companies, like Abbott, have training/certification programs. A pathologist always has to interprete the results, and sign the case. René J.     --- On Fri, 1/22/10, Remy Lopez wrote: From: Remy Lopez Subject: [Histonet] FISH

[Histonet] FISH Qualifications/Training

Good Morning! Does anyone know what are the requirements or qualifications for people to be able to read HER 2 FISH slides? Does it have to be a pathologist? Are there any courses/training available for this? Thanks Remy ___ Histonet maili

RE: [Histonet] fish scales and horse hooves (softening)

tonet-boun...@lists.utsouthwestern.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] fish scales Fish scales are calcified. Depending on the type of fish, they may contain dentin, bone or both. My sources: Two zoology textbooks and also a Wikipedia entry, Scales (zoology) that reads easily. Eve

Re: [Histonet] fish scales

, Canada = = = - Original Message - From: gayle.cal...@bresnan.net Date: Thursday, December 10, 2009 15:50 Subject: Re: [Histonet] fish scales To: "Perry, Margaret" , histonet-boun...@lists.utsouthwestern.edu, "histonet@lists.utsouthwestern.edu" > Are fish scale

RE: [Histonet] fish scales

- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Josie Britton Sent: Friday, December 11, 2009 10:31 AM To: Perry, Margaret; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] fish scales Nair hair remover works great on

RE: [Histonet] fish scales

@lists.utsouthwestern.edu Subject: [Histonet] fish scales We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other

Re: [Histonet] fish scales

also in HistoLogic, author is Jane Chladny. Gayle Callis Sent from my Verizon Wireless BlackBerry -Original Message- From: "Perry, Margaret" Date: Thu, 10 Dec 2009 12:32:09 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fish scales We are trying to cut fish s

[Histonet] fish scales

We are trying to cut fish scales that have been decalcified. They are chunking out when we try to cut them. I think we need to soften the keratin and I looked in the archives for the right dilution of ammonium hydroxide to use. One post said 5% the other said straight. What dilution do you r

AW: [Histonet] FISH on B.M.

, 01. November 2009 10:50 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH on B.M. Helo all, We do FISH assay on FFPE tissue with "spot light tissue pretreatment" of invitrogen. It do works on most of the tissues, but not on Bone Marrow tissue. Do you have any idea?

[Histonet] FISH on B.M.

Helo all, We do FISH assay on FFPE tissue with "spot light tissue pretreatment" of invitrogen. It do works on most of the tissues, but not on Bone Marrow tissue. Do you have any idea? Thanks Dr. Miriam Birnbaum Pathology Asaf Hrofeh Medical Center Israel

RE: [Histonet] FISH probe labeling

du Subject: [Histonet] FISH probe labeling Hi, All We are going to do FISH on mouse tissue. But we have to fluorescent label cDNA probe(~2kb in length) by ourselves because there is no commercial probe is available. Does anyone give me some suggestions? Like which company's product is suitab

[Histonet] FISH probe labeling

Hi, All We are going to do FISH on mouse tissue. But we have to fluorescent label cDNA probe(~2kb in length) by ourselves because there is no commercial probe is available. Does anyone give me some suggestions? Like which company's product is suitable for labeling DNA probe? Which labeling

AW: [Histonet] FISH together with IF

stern.edu] Im Auftrag von Vlazhs Sokols Gesendet: Freitag, 01. Mai 2009 20:21 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH together with IF Dear Histonetters, In the context of Graft-versus-Host-Disease I’m trying to couple immunfluorescence with FISH. I agree, it is a bit dar

AW: [Histonet] FISH together with IF

u] Im Auftrag von Vlazhs Sokols Gesendet: Freitag, 01. Mai 2009 20:21 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] FISH together with IF Dear Histonetters, In the context of Graft-versus-Host-Disease I’m trying to couple immunfluorescence with FISH. I agree, it is a bit daring to do FIS

[Histonet] FISH together with IF

Dear Histonetters, In the context of Graft-versus-Host-Disease I’m trying to couple immunfluorescence with FISH. I agree, it is a bit daring to do FISH and IF at once. The problem is that IF is suffering from the pre-treatment during FISH. After 2 failed stainings I was able to state that primary

[Histonet] FISH-troubleshooting

Hi dear listmembers! What troubleshooting would you suggest for this problem: Her2neu FISH with Vysis-standard protocol (30 min 80°C pretreatment solution, 10 min 37°C 0,05% pepsin) The staining result is clear CEP-staining in green but no LSI-staining in red. With the same run, I had an am