Happy Friday Histoland,
I realize we've had quite a bit of discussion over the past couple of
years about the Special Stain Wash not lasting as long and could possibly be
why sometimes the reason the silvers don't turn out as nice as they used to on
the NEXES special stainer. My que
If you are doing manual special stains, do you have to validate each new
reagent lot number before testing on patient tissue?
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Can anyone using a Dako Artisan give me an idea (ballpark is fine) of what your
cost per slide is running? Thank you in advance!
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Any of them?
If you mean what stain looks pretty, I would say Trichrome on umbilical,
and uvula, hmm, I dunno.
On Tue, May 8, 2018 at 1:05 PM, Charles Riley via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> What special stains can be done on normal uvular tissue and umbilical cord?
>
>
What special stains can be done on normal uvular tissue and umbilical cord?
Trying to put together a small colorful histo project to get people
educated about our field
--
Charles Riley BS HT, HTL(ASCP)CM
Histopathology Coordinator/ Mohs
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Is a retic stain a Group 1 stain or a Group 2 stain? I thought Group 1 was for
micro-organisms and silver stains, but I just read that a retic may be a Group
2 stain.
Laurie Redmond
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Hi everyone,
I am interested if anyone has used histology special stain/IHC/IF images as
artwork for hallways. How large did you print the images, what resolution were
they scanned at, etc. Years ago I came across an article about someone who
created hall panels of special stain artwork. They
Good morning everyone,
I was wondering if anyone can help us in regards to special stain
contamination. We will like to know if contamination can happen as a result of
reagents touching the writing of a marking pen on a slide? For example we are
manually running a slide that has the typical ma
This is an SOP question. I have several older kits that I have made up
in-house solutions to replenish certain stains that have been used up
without having to order a whole new kit. (eg. decolorizer) How do word
the Stain SOP to be put into practice? How often will I have to revise
it officiall
. Cell 410-940-8844
michael.lafrini...@ccplab.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Thursday, May 08, 2014 1:06 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special
Help!
Looking for CAP regs on validating a new platform for special stains. All I
can find is IHC.
Thank you in advance.
Cheryl
Cheryl Kerry, HT(ASCP)
Full Staff Inc.
Staffing the AP Lab by helping one GREAT Tech at a time.
281.852.9457 Office
800.756.3309 Phone & Fax
ad...@fullstaff.o
Hello,
We validate our control blocks prior to patient use. We label the slide with
the stain, date, and results. We keep the slides in a file for reference. Our
recent CAP inspection cited us on this because it was not documented on paper.
I am considering challenging this, as I feel what
If your stains contains a guideline about how many slides can be stained with
them, just follow those guidelines and I am sure you will be fine.
René J.
From: Sophia Lin
To: histonet@lists.utsouthwestern.edu
Sent: Sunday, April 14, 2013 9:47 PM
Subject: [Histonet] special stain reagents change
Coming from a large reference laboratory, it was crucial to change out
reagents for special stains every week since our workload was so heavy. Now
at a smaller GI lab, I am wondering if it is absolutely necessary to change
out the reagents so frequently? We have changed about 2-3 weeks depending
on
I would appreciate any info anyone has about Dako vs Ventana special stain
systems as well as IHC. Thanks so much.
Gloria Tharp, BA, HTL(ASCP)
Director of Operations
PCA Southeast
gth...@pcasoutheast.com
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yce K.
Sent: Monday, August 13, 2012 5:06 PM
To: 'lau...@blufrogpath.com'; Histonet post
Subject: RE: [Histonet] Special Stain documentation
Our techs document that the controls work. My dream would be that the
pathologist would document in the report with a phrase something like "Cont
Laurie-
Our Histotechs document on a stain log that is printed, and the pathologists
comment "controls stained appropriately" within the report.
Nancy
-
Message: 2
Date: Mon, 13 Aug 2012 14:35:22 -0700
From:
Subject: [Histonet] Special Stain documentation
To: "Histonet po
as a
nightmare.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Monday, August 13, 2012 6:06 PM
To: 'lau...@blufrogpath.com'; Histonet post
Subject: RE: [Histonet] Special
tonet-boun...@lists.utsouthwestern.edu] On Behalf Of
lau...@blufrogpath.com
Sent: Monday, August 13, 2012 5:35 PM
To: Histonet post
Subject: [Histonet] Special Stain documentation
How do others document the results of routine special stain controls to be
acceptable before reporting patient results (CAP checklist item
How do others document the results of routine special stain controls to
be acceptable before reporting patient results (CAP checklist item
ANP.21395)? Do the histotechs document the results or do the
pathologists - or both?? If your pathologists document the results, how
do they document them?
wrote:
From: Chiriboga, Luis
Subject: [Histonet] Special Stain query
To: "'histonet@lists.utsouthwestern.edu'"
Date: Friday, June 1, 2012, 2:17 PM
Does anyone have a method/protocol for Pinkus’acid orcein-giemsa stain?
Thanks and good weekend to all
Luis Chiriboga
Ex
Does anyone have a method/protocol for Pinkus’acid orcein-giemsa stain?
Thanks and good weekend to all
Luis Chiriboga
Experimental Pathology Core Laboratory
New York University School of Medicine
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Last October, there was a discussion about being able to bill specials
and Immunos (CPT codes 8312, 88313, 88342, 88360, 88361) per block and
not per specimen. There was a paragraph explaining the change and the
source cited was "CMS /NCCI Updated October 1, 2009." My manager and I
would like to
Just wondering how everyone is formally documenting validation of manual
special stains. If you have a template/form to share that would be
great. Thanks!
Jeffrey C. Birkner, CT(ASCP)
Manager, Pathology Laboratory Section
Collaborative Laboratory Services, L.L.C.
1005 Pennsylvania Ave, Sui
I can provide you with all the H. pylori you could ever want if you have some
good fungus blocks. Let me know if you'd like to trade!
Drew
Sent from my iPhone
On Apr 29, 2011, at 6:20 PM, wrote:
> Hi,
>
>
>
> I am in need of control blocks for special stains, H. Pylori, pneumocystis
> and
Hi,
I am in need of control blocks for special stains, H. Pylori, pneumocystis
and AFB. I am attempting to "grow" our own AFB, but am having difficulty
getting the other control blocks. Commercial vendors charge a LOT of money
for these controls! I was hoping to find a way to swap controls,
Richmond
Sent: Wednesday, December 01, 2010 10:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special Stain Automation
We are in the market for a new stainer to do special stains. We
currently are using the Nexus and have been unhappy with it,
particularly with the silver stains for
Does anyone have experience with the Gemini ES from Thermo Shandon?
-
Avera is a health ministry rooted in the Gospel. Our mission is to make a
positive impact in the lives and health of persons and communities by providing
quality services guided by Chr
-6707
sfe...@cmc-nh.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara
Richmond
Sent: Wednesday, December 01, 2010 10:56 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special Stain
We are in the market for a new stainer to do special stains. We
currently are using the Nexus and have been unhappy with it,
particularly with the silver stains for which we do a lot of. I'm
wondering what techs out there recommend for us to check into.
Good morning everyone,
Does anyone know of a special stain that is specifically for H. billis. I
don't
know much about bacteria, so I am not even sure which bacterial stain would
work
on this.
Any comments would be appreciated.
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
The critical issue that your CLIA, or perhaps CAP, inspector is going to
be asking is: is it necessary to storage stain reagent X in a
refrigerator with a temperature range of Y. That answer lies in your
procedure. If it says refrigerate (perhaps with a range) reagent X,
then you must document t
] On Behalf Of kristen arvidson
Sent: Monday, July 26, 2010 12:08 PM
To: histonet
Subject: [Histonet] Special Stain Storage
Hello All,
We were recently inspected by CLIA and our inspector noticed that we didn't
have reagent storage temperatures written in our Special Stains procedures. We
d
Hello All,
We were recently inspected by CLIA and our inspector noticed that we didn't
have reagent storage temperatures written in our Special Stains procedures. We
do our stains by hand so we do have some stored in the refrigerator and others
stored at room temp. I went through some of the H
Hello my Histonet friends,
Is there any special stain that you know of to demonstrate neutrophils and/or
macrophages in tissue sections?
Best,
Cathy
Cathy M. MathisHistology Core Technician
Wake Forest Institute for Regenerative Medicine
Wake Forest University Health Sciences
Medical Center Blvd.
western.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, June 09, 2010 8:02 AM
To: histonet@lists.utsouthwestern.edu; thisis...@aol.com
Subject: Re: [Histonet] Special Stain Procedures
Neither procedure is reliable. Use Tim's method (sulfur dioxide).
René J.
--- On Tue, 6/8/10, thisis...@
Neither procedure is reliable. Use Tim's method (sulfur dioxide).
René J.
--- On Tue, 6/8/10, thisis...@aol.com wrote:
From: thisis...@aol.com
Subject: [Histonet] Special Stain Procedures
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, June 8, 2010, 4:43 PM
Does anyone hav
the web at:www.archildrens.org
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
thisis...@aol.com
Sent: Tuesday, June 08, 2010 3:43 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special Stain
Does anyone have the following procedures they can share with me:
- Rhodamine (for copper)
- Victoria Blue
Thank you,
Ann
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From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of DeGuzman,
Maria
Sent: Friday, May 07, 2010 6:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Special stain to demonstrate Silver
Hello Histoneters,
Is there a special stai
7232
615-343-9134
Message: 15
Date: Fri, 7 May 2010 07:00:25 -0500
From: "DeGuzman, Maria"
Subject: [Histonet] Special stain to demonstrate Silver
To: "histonet@lists.utsouthwestern.edu"
Message-ID:
<5476245379016b4d8212e8bcd11ecffbb77a5...@amwpvex01.k
Hello Histoneters,
Is there a special stain to detect silver in tissue? If there is please share
the procedure.
Thanks,
Fanny
Maria V. De Guzman| Histology Technician I
Main 908.947.1100 Fax 908.947.1085
Direct 908.947.1482 Email mdeguz...@lifecell.co
Milligan's Trichrome is reputed to be excellent. I have not used it but
colleagues have.
Milligan, M. "A trichrome stain for formalin fixed tissue". Am J. Clin.
Path., Technical Section 10:184-185, 1946.
If you have a copy of Humason's /Animal Tissue Techniques/ the method is
there.
Geoff
She
on control.
René J.
--- On Wed, 2/17/10, Sherwood, Margaret wrote:
From: Sherwood, Margaret
Subject: Re: [Histonet] Special stain for Smooth Muscle Cells
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, February 17, 2010, 10:56 AM
Thanks to all who responded, but I was not specific. I am
Thanks to all who responded, but I was not specific. I am sorry for the
confusion. What special stain would you use (i.e. Masson Trichrome, PTAH, etc.)
(not IHC)
Thanks, again.
Peggy
Peggy Sherwood
Lab Associate, Photopathology
Wellman Center for Photomedicine (EDW 214)
Massachusetts General
Hi everyone,
I'm a lab manager for a small private lab in Arizona. I wish not to use my
work email, since I do not want vendors to contact me. I only wish to
receive some information from users.
Which special stain system do you use and are you happy with it? Why or
why not? Could you p
Hello again fellow histonetters, I have an old artisan special stain/ihc
stain stainer that I'm looking to replace with a newer model, but before
I commit to it I was wondering if anyone else uses any other special
stain instrument that I can look into and compare Thanks for your
help
A
thwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of TIMOTHY MALLOY
Sent: Friday, 12 December 2008 8:52 PM
To: HISTONET ...; TIM MALLOY
Subject: [Histonet] SPECIAL STAIN PNUEMOCYSTSIS
We currently use a stain called grocotts silver methenimine solution for
staining pnuemocystis.
laire
From: histonet-boun...@lists.utsouthwestern.edu on behalf of Rene J Buesa
Sent: Fri 12/12/2008 3:09 PM
To: HISTONET ...; TIM MALLOY; TIMOTHY MALLOY
Subject: Re: [Histonet] SPECIAL STAIN PNUEMOCYSTSIS
I used the MW oven to heat-boost the slutions but the actual staining was done
in a
I used the MW oven to heat-boost the slutions but the actual staining was done
in a water bath. Why don't you try a water bath at 60ºC?
rené J.
--- On Fri, 12/12/08, TIMOTHY MALLOY wrote:
From: TIMOTHY MALLOY
Subject: [Histonet] SPECIAL STAIN PNUEMOCYSTSIS
To: "HISTONET ..."
We currently use a stain called grocotts silver methenimine solution for
staining pnuemocystis. We would like to use a hot plate for the procedure
instead of a unvented microwave. Does anybody have any formula's the would like
to share with the hot plate?
Timothy G. Malloy, HT ( ASCP ) A.A.S.
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