Hello all,
I was wondering if anyone has any experience with doing a trichrome and leaving
it to mordant in Bouin's for longer than 24 hours. Does this overstain and ruin
the stain?
Thanks,
L
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Histonet@lists.utsouthwestern.e
Please clarify if you are referring to the Aniline blue, or the Hematoxylin
blue. I, also, have never heard of using Gills in association with any
Trichrome stain, but as a long time user and educator of using a one-step
(Gomori's) trichrome stain (which is the Leica blue collagen stain), if yo
ipala [mailto:l...@premierlab.com]
Sent: Tuesday, June 30, 2015 3:30 PM
To: Suzanne Martin; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Trichrome troubleshooting
Suzanne
How many times have you used the kit and reagents, I did look up how the kit
works but the trichrome stain can be t
yle Callis
-Original Message-
From: Elizabeth Chlipala [mailto:l...@premierlab.com]
Sent: Tuesday, June 30, 2015 2:30 PM
To: Suzanne Martin; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Trichrome troubleshooting
Suzanne
How many times have you used the kit and reagents, I did l
is Histotechnology Professionals Day
>
>Ship to Address:
>
>Premier Laboratory, LLC
>1567 Skyway Drive, Unit E
>Longmont, CO 80504
>
>
>-Original Message-
>From: Suzanne Martin [mailto:smar...@lcpath.com]
>Sent: Tuesday, June 30, 2015 12:37 PM
>To: histonet@lis
30, 2015 12:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Trichrome troubleshooting
Hi all,
We are having trouble troubleshooting our trichrome. It is too blue. We are
using Leica's kit with the Weigerts iron with Gills. Most of the small bowel
controls have seen improvement b
fessionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-Original Message-
From: Suzanne Martin [mailto:smar...@lcpath.com]
Sent: Tuesday, June 30, 2015 12:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Trichrome troublesho
Hi all,
We are having trouble troubleshooting our trichrome. It is too blue. We are
using Leica's kit with the Weigerts iron with Gills. Most of the small bowel
controls have seen improvement but patient tissue is not... strange.
We have tried lessening the time in Gills, adding time for the
-Ursprüngliche Nachricht-
> Von: Johnson, Carole [mailto:cjohn...@nmda.nmsu.edu]
>
> Gesendet: Freitag, 06. März 2015 15:10
> An: gu.l...@gmx.at
> Betreff: RE: [Histonet] Trichrome & Fixation
>
> Boiuns solution acts as a mordant in trichrome stains
>
>
ition of the term mordant.
Gudrun
-Ursprüngliche Nachricht-
Von: Johnson, Carole [mailto:cjohn...@nmda.nmsu.edu]
Gesendet: Freitag, 06. März 2015 15:10
An: gu.l...@gmx.at
Betreff: RE: [Histonet] Trichrome & Fixation
Boiuns solution acts as a mordant in trichrome stains
-Origina
von Amos Brooks
Gesendet: Donnerstag, 05. März 2015 22:01
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] Trichrome & Fixation
Hi,
It is interesting that you should mention the importance of fixation on
the Trichrome stain. I have an image of two murine hearts processed, cut and
s
Hi,
It is interesting that you should mention the importance of fixation
on the Trichrome stain. I have an image of two murine hearts processed, cut
and stained side by side. The only difference between the two is that they
were harvested at different times, so one sat in formalin long enough
Is anyone having Trichrome Kit Failures on the Ventana Benchmark Special
Stainers. We are running into Kits that present with 10 stains left but we are
finding the mordant has actually run out and the stains will be all blue.
There is no way of knowing this will happen ahead of time. The mach
d
I'm talking about staining by hand.
Best Regards,
Massimo Tosi
"A humble Chemical Engineer who loves Histology"
Da: Cheryl
A: histonet@lists.utsouthwestern.edu
Inviato: Martedì 4 Dicembre 2012 14:18
Oggetto: [Histonet] Trichrome
Hi- need hel
. One final test could be to make up fresh solutions and try again,
although I would suspect you are likely to get more of the same.
All the best,
Amos Brooks
On Tue, Dec 4, 2012 at 1:00 PM,
wrote:
> Message: 3
> Date: Tue, 4 Dec 2012 05:18:35 -0800 (PST)
> From: Cheryl
> Subjec
ber 04, 2012 1:26 PM
Subject: Re: [Histonet] Trichrome
IF you are not successful when the slides are stained on the Nexus OR
manually, while the controls stain well, you can eliminate the instrument as
the cause.
It is most likely a processing artifact: either incomplete fixation, or
overdehydr
instead to your instrument.
René J.
From: Cheryl
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, December 4, 2012 8:18 AM
Subject: [Histonet] Trichrome
Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is
inconsistent. Taking it off the stainer and doing it by hand
: Tom McNemar; 'Cheryl'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Trichrome
Sounds like you may have a cracked vortex mixer and/or silver in your reagent
dispensing line. If the later is the case new tubing will fix this issue and
new vortex mixer(s) will fix the issue w
Sent: Tuesday, December 04, 2012 8:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Trichrome
Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is
inconsistent. Taking it off the stainer and doing it by hand with a kit has
similar results.
The tissue is
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
[tmcne...@lmhealth.org]
Sent: Tuesday, December 04, 2012 12:53 PM
To: 'Cheryl'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Trichrome
Same problem here. Using the Nexus stainer and have also experienced this
Sent: Tuesday, December 04, 2012 8:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Trichrome
Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is
inconsistent. Taking it off the stainer and doing it by hand with a kit has
similar results.
The tissue is
Hi- need help troubleshooting a trichrome. The stain on the Nexus stainers is
inconsistent. Taking it off the stainer and doing it by hand with a kit has
similar results.
The tissue is NBF fixed needle biopsies on kidney. There is a little
mechanical damage on the edges of the tissue but th
I had major problems with frozen trichrome years ago and Jamie Watson from GNF,
La Jolla said to fix in the Bouins and not fix in formalin and then mordant in
Bouins. (duh) I am not sure why I never tried that, but when I fixed in Bouins
for 10 minutes I got consistent trichrome on frozen skin s
Hi,
I have some frozen house hearts that were perfused with 4% paraformaldehyde. I
found protocols for FF, but not fixed frozen. I tried staining them with my
usual Gomori I use on my paraffin blocks but the doc thinks they picked up too
much red, not enough green.
Any suggerstions..I need to g
Hi Drew,
I don't know precisely what causes this, but I have seen it happen when
using a freshly made batch of the dye solution. Also, a very fine
whitish precipitate often collects in the bottom of the bottle in which
the dye solution is stored, requiring filtration every day before using
the so
I'm trying to troubleshoot a problem we had today with a Trichrome stain and
I was wondering if anyone out there could help. We do Gomori Blue Collagen
Trichrome Stain... 10 minutes in Weigert's Hematoxylin and then 15 min in
the Gomori Blue Collagen stain followed by a quick change through acetic
Randy, Our researchers like the modified Goldner's Trichrome. I used a
paraffin method and modified it to work for plastic. A Toluidine Blue works
well on stents, plus the other stains discussed earlier. Vicki
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You can do H&E and VVG quite nicely on this type of MMA embedded
tissue. Maybe you could also try a Sanderson's (methylene blue) with
the Van Gieson (acid fuchsin w/ picric acid) counterstain???
On Dec 21, 2009, at 11:51 PM, Randall Carpenter
wrote:
Dear Histonet,
I was wondering wh
Dear Histonet,
I was wondering what the best Trichrome stain might be for sawn and
ground sections of large stent/artery in methylmethacrylate. I am
also looking to stain elastic fibers. Any suggestions? Thanks.
Randy Carpenter
___
Histonet m
I am having problems with both the Goldners and Massons Trichrome on my MMA
sections. They are ground to between 20 and 30 microns, but the staining
is nonspecific. It stains some of the bone the correct color, but not the
rest of it. Any and all help would be greatly appreciated.
Thanks,
Ric
From: "simoskev...@osteotech.com"
To: histonet@lists.utsouthwestern.edu
Sent: Friday, May 29, 2009 12:58:39 PM
Subject: [Histonet] Trichrome stain in GMA
Does anyone have a good protocol for a Trichrome stain in glycol
methacrylate bone sections. I keep getting no
Does anyone have a good protocol for a Trichrome stain in glycol
methacrylate bone sections. I keep getting non specific staining for
demineralized bone sections. I am using a microwave technique that stains
my top section one way and my bottom section another. I have cut at
different thicknesse
36.
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: [EMAIL PROTECTED]
Date: Monday, November 10, 2008 20:31
Subject: Re: [Histonet] Trichrome Question
To: [EMAIL PROTECTED]
> The contents of this kit is as follows with the timing attached.
>
> Bouins f
ernanAnatomy, UWOLon= don, Canada= =
=- Original Message-From:
[EMAIL PROTECTED]Date: Saturday, November 8= , 2008
10:03Subject: Re: [Histonet] Trichrome Questi onTo:
[EMAIL PROTECTED]>
&g=t;>
> = = >
e, may be added to stain red blood cells. Instructions for
trichrome methods can be found in all textbooks of microtechnique and
histotechnology.
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: [EMAIL PROTECTED]
Date: Friday, November 7, 2008 20:17
Subje
Performed a trichrome stain on a piece of colon which had been in formalin for?
weeks.? Muscle stained blue instead of red.?What happened?? Does prolonged
fixation react this way?
Hope someone can shed some light on this.
Regards,
Angela
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Histonet m
n.edu
Subject: [Histonet] Trichrome Stain
We are going to discontinue the use of the Ventana Trichrome kit because the
kit expires so quickly and we do not perform enough Trichrome stains to use all
of the reagents. We have not performed a manual Trichrome stain in such a long
time that
We are going to discontinue the use of the Ventana Trichrome kit because the
kit expires so quickly and we do not perform enough Trichrome stains to use all
of the reagents. We have not performed a manual Trichrome stain in such a long
time that I was wondering what Trichrome protocol is most la
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