Diana,
I have used the HM355S at two different labs and liked it. Make sure to
demo it or purchase it with the “E” type disposable blade carrier. No one
liked the “ER” type blade carrier. You can see the difference between them
in the operator manual -?just do a browser search for the manual. I
: Thursday, February 29, 2024 3:50 AM
To: histonet@lists.utsouthwestern.edu; Diana Martinez-Longoria
Subject: Re: [Histonet] Question Regarding HM355S Automatic Microtome
Caution: This email came from outside Kaiser Permanente. Do not open
attachments or click on links if you do not recognize
Hi Diana,
We are not currently using the HM355S in our lab, but we did demo it and were
not fans. I would see if you can demo one to see if you like it. We had the
Thermo Shandon Finesse and Finesse Me models and we loved them but the HM355S
didn't appear to be designed the same way and we
Well, I would worry much less about over-fixation than I would under-fixed
tissues. Do you not have the option to have a weekend processing schedule that
runs RT formalin?
Is your volume sufficient you could do an analysis of weekend vs non-weekend
ER, PR, HER2 rates and see if they are
Howdy Lisa and Histonet List Members,
It is permitted. I think the membership benefits from knowing what
opportunities exist in the field.
Job postings via recruitment services, as well as those from individual
hospitals, commercial, or academic labs are welcome.
Logistics on how to
Hi Richard!
That is the duplicate of what we do in our lab. We were putting both the
core and the clot on the same slide for in house testing, but den outs we
sometimes have to split the specimen and only on a slide.
I have a question for you about ISH Kappa/Lambda. What decal protocol do
you
Hi Martha,
We have been using Beaker for over 2 years now. We created another accession
that identifies cases that are being sent to us as a consult/referral (both
Cyto and Histo). It works pretty well for us.
Scott
Scott A. Lindrud, MLS(ASCP)CT | Histopathology Technical
Martha, we give it the same number sequence as any other surgical or cytology
case, but we can identify our cases by "Specimen Class" so consults get a
specimen class according to the type of consult - cyto, cytogyn, surgical,
etc. The specimen class is printed on the labels for all
We identify our outside surgical consults as OC, for example OC20-00010
Les Raff
On Thu, Dec 10, 2020 at 12:45 PM Martha Ward-Pathology via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> We are in the process of switching to AP Beaker and in the midst of the
> build.My question
Hi
I have done several yrs of work on Eisenia fetida worms with a PhD student.
I decided to do a std Pwax processing schedule.
The student dissected the appropriate area then placed in 10% Formalin in PBS
pH7.4 for 24hrs on a rocker ( gentle agitation)
I then processed to Pwax using a std
: Re: [Histonet] Question concerning H. pylori staining
Hi!
I found this instruction for a Hp- stain, that sounds similiar to yours.
They want the slides to be airdried after water-rinsing and before xylen.
But the result should be blue bacteria, not purple.
I would try to let the slides air-dry
with
Alcian yellow, to give a more contrasted result.
Gudrun Lang
-Ursprüngliche Nachricht-
Von: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Donnerstag, 9. April 2020 20:05
An: Histonet@lists.utsouthwestern.edu
Betreff: Re: [Histonet] Question concerning H
Michelle (where) asksi: >>We have a question about staining for H-Pylori
Using Quick Stain (Periodic acid 1%, Alician Yellow, Sodium Metabisulfate,
Toluidine Blue stock, Sodium Hydroxide) we notice what clearly looks like
the H-Pylori purple stained clusters, but after dehydration in 100% alcohol
Thank you Victoria
Regards
Ranna
Sent from my iPhone
> On Apr 8, 2020, at 4:39 AM, Victoria Baker wrote:
>
>
> Ranna,
>
> I took the course and it was well worth the cost for me. As to how it is
> assisting my career right now it isn't as my facility stopped using imaging
> about a
Ranna,
I took the course and it was well worth the cost for me. As to how it is
assisting my career right now it isn't as my facility stopped using imaging
about a year and a half ago. With the pandemic they may be rethinking
this, but it won't be immediate.
This type of skill will be more
Gelatin embedding is easy. You infiltrate the specimen and then fix it again in
formaldehyde to cross-link the gelatin molecules and make the whole mass
isoluble in water. You can than cut frozen sections of any kind: cryostat, or
with an old-fashioned freezing microtome collecting thawed
Hi Dr. Cartun,
It has been many years since I worked in EM but I my recollection is that
tissues could remain in 2% Glut indefinitely without detriment (for EM
purposes). However, Osmium tetroxide had to have a limited exposure.
Greg
--
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE C0A
RBS is the same as Methylene Blue, I believe.
_
Jessica Riggleman | Research Associate
Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:
Hi Tim,
In the hospitals I have worked, we used the PAD simply to communicate gross
pathologic diagnoses, so we would issue a gross description of the brain (like
the brain weight compared to normal, edema, atrophy, herniation, hemorrhage,
etc), "pending microscopic examination", and then
With regards to the HistoOrientator, it does removes wrinkles from the sections
fairly effectively. That being said, I would not use it on any sections
intended for immunohistochemistry. The hot plate on this device gets very hot
and would no doubt damage or affect the antigenicity of such
You can dewax absolutely safely using a 2% dishawasher soap solution at 90ºC
(twice) as washing in water.You can "dehydrate" stained stains by placing the
slides in an oven at 60ºC, also absolutely safely for the stained section.Under
separate cover I am sending articles on this subject.René
All that matters here is the final concentration of the reagent - it doesn't
matter what stock you start with if you calculate the dilution.
Adding 1.25 ml to 1000 ml is diluted by a factor of .00125 so 10N x .00125 =
.0125 N final concentration.
If using a 1 N stock solution, just add 10X the
Huh?
Take a solution more dilute than you want it and dilute it more?
Geoff
On 5/1/2015 10:41 AM, Goins, Tresa wrote:
All that matters here is the final concentration of the reagent - it doesn't
matter what stock you start with if you calculate the dilution.
Adding 1.25 ml to 1000 ml is
The final concentration of 1.25 ml 10N NaOH into 1000 ml water is the same as:
12.5 ml 1N NaOH into 987.5 ml water.
-Original Message-
From: Geoff [mailto:mcaul...@rwjms.rutgers.edu]
Sent: Friday, May 01, 2015 9:16 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Question
Hi Debra,
My experience differs from Elizabeth and others. Indeed have made thousands of
mouse bone preparations with formic acid decaled sections. Here is an article
that didn't copy paste so well:
RANK is the intrinsic hematopoietic cell surface
receptor that controls
Cyto
Sent from my Verizon Wireless 4G LTE smartphone
Original message
From: Pratt, Caroline caroline.pr...@uphs.upenn.edu
Date:02/19/2015 4:59 PM (GMT-05:00)
To: 'histonet' histonet@lists.utsouthwestern.edu
Subject: [Histonet] Question about POC testing
Does anyone
Hi Ryan,
thanks a lot for your thoughts. These blocks were processed elsewhere
and sent to us for the cutting and staining. Tissues were dehydrated in
five consecutive baths of ethanol 100%, 5 hours each (manual
processing). Then, they went to xilene (three baths, 4 hours each) and
paraffin
] On Behalf Of Julio Benavides
Sent: Friday, February 13, 2015 10:19 AM
To: histonet@lists.utsouthwestern.edu
Subject: [EXTERNAL] RE: [Histonet] Question regarding dehydration
Hi Ryan,
thanks a lot for your thoughts. These blocks were processed elsewhere and sent
to us for the cutting and staining
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Julio Benavides
Sent: Friday, February 13, 2015 8:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [EXTERNAL] RE: [Histonet] Question regarding dehydration
Thanks Ryan. Tissu sections where thin, something
NO, because the license in histotechnology INCLUDES high complexity testing.
On the other hand there are some tests like FISH with the VYSIS system that
requires a special training with its own certification.
René J.
On Tuesday, April 29, 2014 10:15 PM, Delia, Catherine deli...@uhnj.org
wrote:
We used to do TEM at our lab. The work volume was not high and one of
histotechnologists was trained to do ALL tasks (fixation → printing the photos
of the areas selected by the pathologist).
When not doing TEM tasks, he took care of any of any of all HTL tasks for which
he was also trained.
It's the same for me here. But then again, we're pure research.
Kathleen
Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
We used to do TEM
Hi Peggy,
In my opinion, it depends upon the objective of the study. Cell autolysis
begins shortly after death as a result of lack of oxygen supply as well as
nutrients. The membranes of the lysosomes break down, and the acid hydrolases
begin to degrade the cellular
If you're merely looking
Surely I will find those that disagree with this post, however what I was
classically trained about fixation categories generally falls within the
information below...which I took the liberty of reposting here since it is
pretty clear straightforward from Leica.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Grantham,
Andrea L - (algranth)
Sent: Thursday, August 01, 2013 4:52 PM
Cc: HISTONET
Subject: Re: [Histonet] Question - OR specimens to Pathology
OR - you can get
Richard
MOPEC has an item called pathport it's about the size of a tool box that what
we used to use to carry frozen sections from the surgery to pathology.
http://www.mopec.com/product/1829/pathport/
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder,
OR - you can get a rubbermaid took box and slap a few biohazard stickers on it
and put some formalin pads inside and you have a transport box for surgical
specimens.
I happen to know that that is how this box was invented.
Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of
: Grantham, Andrea L - (algranth) algra...@email.arizona.edu
To:
Cc: HISTONET histonet@lists.utsouthwestern.edu
Sent: Thursday, August 1, 2013 3:51 PM
Subject: Re: [Histonet] Question - OR specimens to Pathology
OR - you can get a rubbermaid took box and slap a few biohazard stickers
out, vaccum and
rinse steps and air dry steps needed to decontaminate some ( usually older)
cryostats.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: abri...@brightinstruments.com
Date: Wed, 20 Mar 2013 21:37:45 +
To: marilyn.a.we...@kp.org
Subject: Re: [Histonet] question
CC
...@brightinstruments.com
Subject: Re: [Histonet] question
Date: Thu, 21 Mar 2013 10:25:41 +
To: joellewea...@hotmail.com
I do not see that as UV will not decontaminate areas and tissue that are not in
the UV light path or thick tissue.Alan Bright
Sent from my iPhone
On 20 Mar 2013, at 23:38, joelle
Strange about the gloves when vacuuming out fresh tissue trimmings from
cryostats and exhausting the air back into the lab is done.
Alan Bright
On 20 Mar 2013, at 19:07, marilyn.a.we...@kp.org marilyn.a.we...@kp.org
wrote:
Thank you for your response about the squames and we are all wearing
To: marilyn.a.we...@kp.org
Subject: Re: [Histonet] question
CC: histonet@lists.utsouthwestern.edu
Strange about the gloves when vacuuming out fresh tissue trimmings from
cryostats and exhausting the air back into the lab is done.
Alan Bright
On 20 Mar 2013, at 19:07, marilyn.a.we...@kp.org
sorry for the misspelling in my previous post.
Joelle Weaver MAOM, HTL (ASCP) QIHC
From: joellewea...@hotmail.com
To: abri...@brightinstruments.com; marilyn.a.we...@kp.org
Date: Wed, 20 Mar 2013 23:38:04 +
Subject: RE: [Histonet] question
CC: histonet@lists.utsouthwestern.edu
I'm new to the Histonet website, but not new to histotechnology.
I'm trying to salvage some irreplaceable Zucker Diabetic Fatty rat tissue
samples that were embedded in paraffin in May 2009. They have sat on my bench
since they were embedded as we thought the entire experiment was a huge
Natalia:
I just cannot understand the concept of gravity convection oven because if
you say gravity by definition that will mean that the heat will work by
gravity and any heated air goes, also by definition, against gravity because
any heat air will go UP and not down, hence the hot air
; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Question about slide drying/ convection ovens
Natalia:
I just cannot understand the concept of gravity convection oven because if
you say gravity by definition that will mean that the heat will work by
gravity and any heated air goes, also
Yes!
René J.
From: Courtney Pierce courtney.pie...@quintiles.com
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu
Sent: Tuesday, January 22, 2013 1:04 PM
Subject: [Histonet] Question
Are IHC high complexity test.
Courtney Pierce
IHC Specialist
Quintiles
Translational RD
The staining portion is not high complexity. The reading of the slide is.
On Tue, Jan 22, 2013 at 10:04 AM, Courtney Pierce
courtney.pie...@quintiles.com wrote:
Are IHC high complexity test.
Courtney Pierce
IHC Specialist
Quintiles
Translational RD - Oncology
Innovation
Navigating the
As in any e-mail, once you press send, you cannot withdraw/remove the message.
You may try to write to the master of the site.
René J.
From: Chris Winans ch...@bakopathology.com
To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu
Sent: Monday, January 21, 2013 7:59 AM
The sectioning protocols/sequences are determined by the pathologists and what
they think is required to make diagnoses. We also stored the slides with extras
sections until the case is signed and get an additional OK from the
signing pathologist before disposing of the unused sections.
I would
Formalin fixed tissue is not adequate for frozen sectioning unless you place it
in sucrose first before going into OCT and frozen sectioning. Spleen is
particularly difficult for its high blood contents. Ideally you should try to
obtain unfixed tissue.
René J.
What you describe (NBF fixation → band saw slicing → decal with formic acid →
end point with 5% ammonium oxalate) seems a good protocol BUT you have to pay
special attention to:
1- Did you fix the femurs in toto? If you did that, fixation is extremely
difficult. You first should select a
I have never re-accessioned a case for this. I know that I have went
back at least two years and billed a case for additional studies.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard
Cartun
Sent:
Rich, From 30 days after signout we require a new accession number or addendum
(pathologist decide which to do depending on circumstances. Generally we prefer
to make an addendum to the original report). For addendums, since we can't add
new tests to the old accession number, the work is done
If I understand correctly(and this is how we do it)
A case is considered archived after 30 days. Within that 30 days, you bill
the same account, which adds late charges.
IF the case is pulled for a review to perform MOLECULAR testing, you can charge
an 88363 for that review at any time for
I think your radiology dept suppose to do the radiological exam on the core
breast biopsies after the specimen is taken from the patient, radiology bills
for this service, they only send the x ray film to the histology lab for
pathologists to identify the calcified area of the breast.
Tunde
On Fri, Mar 9, 2012 at 10:14 AM, Tunde Ajibade tajib...@echd.org wrote:
I think your radiology dept suppose to do the radiological exam on the
core breast biopsies after the specimen is taken from the patient,
radiology bills for this service, they only send the x ray film to the
histology
Ward's Natural Science.
On Mon, Mar 5, 2012 at 7:43 PM, Komal Gada kjg...@gmail.com wrote:
Hello Histo-netters,
I'm looking for a source which sells slides for a Tissue Identification
class. Does anyone have any leads on where to go for something like this?
Thanks,
Komal
responded so far :)
From: tony.henw...@health.nsw.gov.au
To: karabo...@hotmail.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Question regarding staining of ligament tissue using
HE. The nuclei in our ligament tissue is not staining consistently.
Date: Mon, 23 Jan 2012 22:38:28
Have a look at the dewaxing part of the protocol.
Is the xylene removing all the wax?
If wax is incompletely removed from the sections then nuclei will be poorly
stained whereas, interestingly the eosin counterstain will seem to be
unaffected (though with a diligent look you will see poorer
The Known Error Test' comes for a company:
http://www.knowerror.com
They offer a product that utilizes a biopsy collection system with a swab,
utilizing chain of custody protocols. They claim that they can prevent patient
identification errors because of specimen provenance complications.
Have you tried gall bladder? And some times livers that are diseased have it.
They always look green.
Just a thought.
Sent from my iPhone
On Dec 20, 2011, at 3:45 PM, Thurby, Christina christina.thu...@bms.com
wrote:
Hello All,
I have a few questions about Bile Stain control tissue:
Sorry. There is not.
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
[rcar...@harthosp.org]
Sent: Monday, December 05, 2011 12:17 PM
To: Histonet
Subject: [Histonet] Question - CPT
Your waterbath is not hot enough!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia
Spencer
Sent: Tuesday, November 29, 2011 10:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]
When you rotate the tissue basket holder to the 1st formalin, it prevents
someone from forgetting to do that when they start the processor later. If it
doesn't get rotated, the unprocessed tissues will drop right down into
paraffin. It just must be rotated either at the end of a run (which is
After repeating so many times the same sequence anybody can get confused,
either you or your supervisor.
Forget about verbal/personal instructions and follow the manual. It was
developed to obtain a perfect sequence always.
René J.
--- On Wed, 11/23/11, Jenny Vega histotech...@gmail.com wrote:
Hi,
I use labels and ribbons from this company. They do not wash off or fade.
http://www.barcode-labels.com/solutions/laboratory-barcode-systems
Labels part# 92169 and ribbon #T84252074
Paula K. Pierce, HTL(ASCP)HT
President
Excalibur Pathology, Inc.
8901 S. Santa Fe, Suite G
Oklahoma City,
We do either IHC or a Modified Steiner, depending on the Pathologist's
preference. We switched to Newcomer Supply's Steiner-Chapman Modified
Silver Stain Kit about a year ago, because it eliminated the use of
Uranyl Nitrate. We have been delighted with the consistency of the
stain.
Sandy
-requ...@lists.utsouthwestern.edu
Subject: RE: [Histonet] question on H pylori
We do either IHC or a Modified Steiner, depending on the Pathologist's
preference. We switched to Newcomer Supply's Steiner-Chapman Modified
Silver Stain Kit about a year ago, because it eliminated the use of Uranyl
Nitrate
We use IHC on cases that show the appropriate inflammatory background. Please
note that histochemical stains may not identify intracellular H. pylori.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic
IHC here.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
From: Setlak, Lisa lset...@childrensmemorial.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu;
histonet-requ...@lists.utsouthwestern.edu
...@lists.utsouthwestern.edu
Subject: Re: [Histonet] question on H pylori
IHC here.
Sheila Haas
Laboratory Supervisor
MicroPath Laboratories, Inc.
From: Setlak, Lisa lset...@childrensmemorial.org
To: histonet@lists.utsouthwestern.edu histonet
Modified Steiner.
René J.
--- On Tue, 6/7/11, Setlak, Lisa lset...@childrensmemorial.org wrote:
From: Setlak, Lisa lset...@childrensmemorial.org
Subject: [Histonet] question on H pylori
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,
IHC here.
On Tue, Jun 7, 2011 at 11:12 AM, Setlak, Lisa
lset...@childrensmemorial.org wrote:
I was just curious what everyone is using for standard of care regarding H =
Pylori..is everyone doing IHC or are you doing a Giemsa?
Thanks,
Lisa
Lisa M. Van Valkenberg, B.S., HT-
In MS, it's a problem of not having anyone to fill the vacant positions. And
once you find someone, that person can pretty much say the hours she/he wants
and the pay b/c you have no other candidates to choose from.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
The easiest way to remember is to count the midnights between starting and
ending - therefore today will be 0, tomorrow 1etc
On Mon, Apr 18, 2011 at 6:24 PM, Jenny Vega histotech...@gmail.com wrote:
Yes, that is what makes sense to me. Tomorrow morning after removing the
specimens from the
She is right.
Day 0 is today (the same day you start the run) -day 1
Day 1 is tomorrow start the run the next day,- day 2
Day 2 is the day after tomorrow - day 3
Day 3 is the following day -day 4 and so on
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] Question about delayed start in Leica Tissue Processor
She is right.
Day 0 is today (the same day you start the run) -day 1 Day 1 is tomorrow start
the run the next day,- day 2 Day 2 is the day after tomorrow - day 3 Day 3 is
the following day -day 4 and so on
-Original
Yes, that is what makes sense to me. Tomorrow morning after removing the
specimens from the tissue basket I will check how my supervisor set the
clock last Friday. If she chose 3-18:20, then by logic I will have to chose
4-18:20, since I will be away for the holidays for 4 days straight and I
Yes. After the final report is signed out, any changes, additions, or
corrections have to be put through as an addendum. This becomes part of the
patient's permanent record.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
While not a cell membrane marker, you can often use differential
interference contrast (DIC) microscopy. The microscope I use has a special
setting that lets you add DIC without any additional staining. In the mouse
bone marrow paraffin sections, it lets you see most edges of most cells.
Adam
On
Hi, Teri,
How about this? It also gives a brief blurb in the description about
commonly used alternatives, if this isn't your thing. I've never tried it,
so I can't vouch for it myself, but Invitrogen does make some pretty darn
good stuff...
http://probes.invitrogen.com/media/pis/mp10045.pdf
We have had the ultras for over a year now and that is a new one. Was the rack
of antibodies not seated properly? Just a thought.
Stacey
Sent via BlackBerry from T-Mobile
-Original Message-
From: Gudrun Lang gu.l...@gmx.at
Sender: histonet-boun...@lists.utsouthwestern.edu
: Friday, November 12, 2010 11:22 AM
To: gu.l...@gmx.at; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] question to ventana benchmark XT and ultra users
We have had the ultras for over a year now and that is a new one. Was the rack
of antibodies not seated properly? Just a thought
Just use Mayo (not fat free, of course!) and smear it on the slide like you
would a blood smear. It stains beautifully.
Drew
On Wed, Oct 6, 2010 at 09:15, Komal Gada kjg...@gmail.com wrote:
Hello Histonetters,
I am trying to find a procedure for using butter and egg yolks as controls
for
Komal,
I don't know what kind of lab you are in, I'm in a core facility and I
do histology on research projects. When I get an ORO this is what I do
for a control:
I get a piece of tissue like mouse kidney with some fat attached or
maybe some muscle with fat and have it snap frozen. I
@lists.utsouthwestern.edu
Subject: Re: [Histonet] Question about Oil Red O controls
Komal,
I don't know what kind of lab you are in, I'm in a core facility and I do
histology on research projects. When I get an ORO this is what I do for a
control:
I get a piece of tissue like mouse kidney with some fat
The volume of a mouse is about 25ml, of which perhaps 2ml is blood. 5ml of
saline (or PBS) is enough to wash it all out. Some people precede this with a
small bolus (about 0.2ml) of 1% aqueous sodium nitrite to dilate the blood
vessels. After washing out the blood, perfuse with about 10 ml of
Hi Li,
Try 1-2 ml/min perfusion rate. Yes 10ml/min is way too fast. I know it's
tricky; I've done it by hand and by pump.
Perfuse with PBS or Saline (same thing) first, then 4% PFA til mouse is
stiff, then after taking the brain out immerse it in increasing grades of
sucrose
Go to a library and see if the book has the information you need.
Decide whether to photocopy a few pages (for a specific job) or
ask your lab to buy a copy of the book as a resource for all who
work there. Every lab needs several books, and provision of time
for the workers to read them.
If you process the control in any different way than the case slides, it is no
longer an acceptable control for that test.
Remember that the control is used to determine if the reaction took place when
used simultaneously and in an identical way as the test slides.
René J.
--- On Fri, 1/8/10,
We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE
HISTOPATHOLOGY LABORATORY, by Dapson Dapson, fourth edition, pg 184.
1. Prepare the following aqeous stock solutions:
a. 0.2M potassium permanganate (3.16% or 31.6g KMnO4/liter)
b. 2.0M sulfuric acid (11.2% or 112 mL
] On Behalf Of Lynette Pavelich
Sent: Wednesday, May 13, 2009 12:10 PM
To: histonet@lists.utsouthwestern.edu; jcampb...@vdxpathology.com
Subject: Re: [Histonet] Question about DAB waste
We detoxify our DAB using this method from: HAZARDOUS MATERIALS IN THE
HISTOPATHOLOGY LABORATORY, by Dapson Dapson
Alyssa,
As Director of a large AP lab here in Maryland, to answer your question to the
best ability, one would need additional information.
Currently under accreditation guidelines a Cytotechnologist is allowed to read
100 non imaged slides per 24 hour period, or 200 {negative} imaged
Our cytotechs screen 40 to 60 a day.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Alyssa
Peterson
Sent: Thursday, April 02, 2009 3:49 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet]
Hi Jenee: You need to check the histonet archieves. For the last two weeks
there has been an on going conversation in regard to disposal of microscopic
slides. Since I am in a COR lab most of my slides are sent to the PI's that
order the slides therefore I do not have to deal with this as of
,
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Subject RE: [Histonet] question regarding rules for disposal of
slides = cassettes
Hi Jenee: You need to check the histo= net archieves. For the last
two weeks there has been
We also don't keep melted paraffin in the holding chamber of the embedding
center. We keep the chamber just a little warmer than the melting point of the
paraffin, been doing it this way for as long as I have been working here at the
Veterinary diagnostic lab at KSU ( around 20 yrs). I also
I've worked in several places and only one actually kept the tissues in hot
paraffin at the embedding station and usually had alot of complaints on the
brittleness of the tissues. I agree that there is no need for extra
cooking time in melted paraffin, but I have learned a nice trick from a
I was starting to feel all alone in the world, Rene'!
First, let me say that animal tissue always seems a littler drier to me and I
typically do work with human (neuro) tissue these days. I've never had a
problem leaving cassettes in molten wax, as long as the processing was adequate
and the
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