Hi Christopher, just a remark: for phenix.refine it does not matter where the flags come from and what is the "test"/"work" value since it automatically scores the values in the flags array and guesses the right one. Still one can imagine corner case, so it's good to be careful -:)
Pavel On Fri, Dec 9, 2011 at 2:08 AM, Christopher Browning < christopher.brown...@epfl.ch> wrote: > Hi Everybody, > > First off, thanks for the replies. They definitely fixed my problem. It > was indeed as Garib Murshudov said. The flags got swapped, and therefore > the percentage of Rfree reflections were 95%. > > So, if Rfree is created from CCP4.....use Rfree flags with a value of 0 > and a value of 1 if the Rfree was created with PHENIX. > > Both maps now look the same, and it indeed looks like the protein in the > crystal is different from the sequence. > > Cheers, > > Chris > > > > > -- > Dr. Christopher Browning > Post-Doctor to Prof. Petr Leiman > EPFL > BSP-416 > 1015 Lausanne > Switzerland > Tel: 0041 (0) 02 16 93 04 40 >
