Dear Careina,
Unfortunately there is no direct connection between nice looking crystals and good diffraction. Sometimes ugly crystals can diffract well while nice ones don’t. You may try crystal dehydration ; it allows to remove excess of solvent and change crystal packing which can lead to better crystal order and better diffraction. Here are some examples described in the literature https://www.ncbi.nlm.nih.gov/pubmed/24311593 https://www.ncbi.nlm.nih.gov/pubmed/22232185 https://www.ncbi.nlm.nih.gov/pubmed/12575933 Bests, Zaineb Le mardi 14 août 2018 à 12:11:23 UTC+2, Hughes, Jon <jon.hug...@bot3.bio.uni-giessen.de> a écrit : #yiv5746868635 #yiv5746868635 -- _filtered #yiv5746868635 {font-family:Helvetica;panose-1:2 11 6 4 2 2 2 2 2 4;} _filtered #yiv5746868635 {panose-1:2 4 5 3 5 4 6 3 2 4;} _filtered #yiv5746868635 {font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;}#yiv5746868635 #yiv5746868635 p.yiv5746868635MsoNormal, #yiv5746868635 li.yiv5746868635MsoNormal, #yiv5746868635 div.yiv5746868635MsoNormal {margin:0cm;margin-bottom:.0001pt;font-size:12.0pt;font-family:New serif;}#yiv5746868635 a:link, #yiv5746868635 span.yiv5746868635MsoHyperlink {color:blue;text-decoration:underline;}#yiv5746868635 a:visited, #yiv5746868635 span.yiv5746868635MsoHyperlinkFollowed {color:purple;text-decoration:underline;}#yiv5746868635 p {margin-right:0cm;margin-left:0cm;font-size:12.0pt;font-family:New serif;}#yiv5746868635 p.yiv5746868635msonormal0, #yiv5746868635 li.yiv5746868635msonormal0, #yiv5746868635 div.yiv5746868635msonormal0 {margin-right:0cm;margin-left:0cm;font-size:12.0pt;font-family:New serif;}#yiv5746868635 span.yiv5746868635E-MailFormatvorlage19 {font-family:sans-serif;color:#2E74B5;}#yiv5746868635 .yiv5746868635MsoChpDefault {font-size:10.0pt;} _filtered #yiv5746868635 {margin:70.85pt 70.85pt 2.0cm 70.85pt;}#yiv5746868635 div.yiv5746868635WordSection1 {}#yiv5746868635 maybe it's the cryobuffer that's the problem (you didn't mention it). you could try to fish the crystals with minimal liquid attached by mounting them in oil rather than a cryobuffer. or you could test the native diffraction "in situ" (at room temperature in the drop): quite a few beamlines offer this possibility these days. best jon Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK]Im Auftrag von Careina Edgooms Gesendet: Dienstag, 14. August 2018 11:59 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] crystals that dont diffract :( :( I got the most beautiful crystals I have ever seen and they don't diffract at all. Not poor diffraction, NO diffraction. Anyone know why this could be and how I can go about fixing it? I had three beautiful crystals and not one diffracted. I did leave them in the drop for about 3 weeks before harvesting and in liquid nitrogen for about a month before diffracting. Could that be a factor? If I regrew more beautiful crystals and diffracted straight away could that help? Careina To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
